ライフサイエンスコーパス: transposon insertion

1 c regions showing significant enrichment for transposon insertion.

2 tein (CRP) was identified as the site of the transposon insertion.

3 ed-cut palindromic target site model for DNA transposon insertion.

4 n the first selection are homozygous for the transposon insertion.

5 ency production of clones harboring a single transposon insertion.

6 ertion sites identified 7 genes activated by transposon insertions.

7 selective advantage for cells with multiple transposon insertions.

8 ns, more than two-thirds of which are due to transposon insertions.

9 ed to remove what are likely the remnants of transposon insertions.

10 le-induced locomotion that are caused by the transposon insertions.

11 marks" at intergenic sites known to tolerate transposon insertions.

12 ation of genes in lines carrying Spm or dSpm transposon insertions.

13 e as a high-throughput technique for mapping transposon insertions.

14 eas the amino terminus of VP19C can tolerate transposon insertions.

15 sion loop of the E protein was intolerant of transposon insertions.

16 on such as recO, recQ, and a cis-acting pilE transposon insertion all rescue the RecA-dependent growt

17 Here we report a unique transposon insertion allele in a small ORF located immed

18 ascicular fibers, as previously seen in tnt1 transposon insertion alleles.

19 om the gene sequence that is duplicated upon transposon insertion, allowing perfect splicing out of t

20 n in ISA1, and isa2-339, which was caused by transposon insertion and conditioned loss of ISA2.

21 genomes, we describe a new method called the Transposon Insertion and Depletion AnaLyzer (TIDAL) and

22 Thus, transposon insertion and sequence co-option may explain

23 genic region, while FT2c and FT2d obtained a transposon insertion and structural rearrangement, respe

24 Rates of Mu transposon insertions and excisions are both high in lat

25 olanaceous R genes revealed the magnitude of transposon insertions and local duplications that result

26 relation of methylation-poor regions with Mu transposon insertions and recombination, and copy number

27 We show that IM-Fusion accurately identifies transposon insertions and their true target genes.

28 vrC, uvrD, and mfd) were each disrupted by a transposon insertion, and the uvrB and uvrD mutants were

29 ater fish express more GDF6 due in part to a transposon insertion, and transgenic overexpression of G

30 pair expansions of 2-15 bp repeat units, (4) transposon insertions, and (5) INDELs containing random

31 k1 and pdk2 were inactivated individually by transposon insertions, and both genes were simultaneousl

32 of sequence upstream of pilE were devoid of transposon insertions, and some deletions in these regio

33 Thus, a natural transposon insertion appears to mediate an ecologically

34 Recurrent sites of transposon insertion are commonly identified using ligat

35 The locations of transposon insertions are highly reproducible and agree

36 These 17 transposon insertions are located in nine open reading f

37 S regions, direct transcriptional effects of transposon insertions are observed.

38 sertion lines showed that the effects of the transposon insertions are often dependent on development

39 Maize lines lacking RAF1 due to Mutator transposon insertions are Rubisco deficient and seedling

40 with pigmentation distinct from the initial transposon insertions as a consequence of germline trans

41 llection of mutant strains containing single transposon insertions associated with different genes.

42 different pools were identified, each with a transposon insertion at a different position within the

43 dating the cooperation between JAK2V617F and transposon insertion at the Erg locus in the JAK2V617F-p

44 ng, and that the loss of silencing caused by transposon insertions at plant Pc-G targets reflects imp

45 An M. marinum strain bearing a transposon-insertion between the MMAR_1663 and MMAR_1664

46 he number and fraction of rDNA units without transposon insertions, but not with total rDNA locus siz

47 We aimed to retarget transposon insertions by comparing a series of novel hyp

48 We screened mutants with non-redundant transposon insertions by fluorescence-activated cell sor

49 However, genetic changes such as transposon insertions can also lead to changes in DNA me

50 Transposon insertions can be nested and make the task of

51 ntial PA14 genes are represented by a single transposon insertion chosen from a comprehensive library

52 Recurrent transposon insertions could be mapped to genes in the JA

53 refinement in the interpretation of observed transposon insertions demonstrated that genes without in

54 ic datasets representing different levels of transposon insertion density and sequence coverage.

55 Evidence indicates that this transposon insertion, designated CRISPR3*, is a gain-of-

56 Other transposon insertions disrupted acetoin biosynthesis (th

57 We previously reported that a transposon insertion disrupting rpoE resulted in the dec

58 Statistical analysis of transposon insertion distribution revealed a set of 453

59 wed Kolmogorov-Smirnov (K-S) test to analyze transposon insertion distributions in sequence windows o

60 -oriented approaches in recovering validated transposon insertion events.

61 A transposon insertion FlhDC(-) mutant produces very low l

62 ns, and gene essentiality calls were made by transposon insertion frequency analysis (TIFA).

63 Cloning of transposon insertions from lymphomas/leukemias identifie

64 Genetic screening using random transposon insertions has been a powerful tool for uncov

65 Analysis of over 16,000 transposon insertions identified 77 candidate CRC genes,

66 tion sites in these lesions, and analysis of transposon insertions identified candidate prostate canc

67 Analysis of transposon insertions identified eight genes including B

68 In this study, we identified a transposon insertion in a novel gene, designated disA, t

69 fied a noncytotoxic M. marinum strain with a transposon insertion in a predicted N-alpha-terminal ace

70 he leech, we found one mutant, JG752, with a transposon insertion in an ascU homolog, encoding an ess

71 ular interest, since this mutant contained a transposon insertion in an intergenic region between BAs

72 One putatively hypervirulent strain with a transposon insertion in an intergenic region was identif

73 Transposon insertion in ccpA also restored proline proto

74 nonmotile mutant of C. jejuni 81-176 with a transposon insertion in Cj1026c, but verification of the

75 We previously found that a transposon insertion in Cjj81176_1038, encoding a homolo

76 We identify a transposon insertion in fhuB, a gene that encodes a ferr

77 In addition, transposon insertion in five other gammaHV68 open readin

78 tional fusion resulted in the isolation of a transposon insertion in glmS, encoding the essential enz

79 es, but no bovine isolates, showed an IS1548 transposon insertion in hylB, which encodes a hyaluronid

80 entified a multidrug-sensitive mutant with a transposon insertion in lpqB, the gene located immediate

81 A to the bacterial pole, a mutant carrying a transposon insertion in mreB displayed altered targeting

82 The phenotype of cells containing a transposon insertion in mreB was indistinguishable from

83 This cluster was identified by a polar transposon insertion in orf2 that resulted in a strain d

84 AB307.27 contained its transposon insertion in pbpG, which encodes the putative

85 contrast to the case for this femAB mutant, transposon insertion in SAV2335, herein named lyrA (lyso

86 Erg) and Ets1 were the most common sites for transposon insertion in SB-induced JAK2V617F-positive er

87 rpels (foc), an Arabidopsis mutant with a Ds transposon insertion in the 3' regulatory region of MIR1

88 xpression of the nuclear CBT1 gene, due to a transposon insertion in the 5'-untranslated region, resc

89 of A. actinomycetemcomitans that contained a transposon insertion in the Aae structural gene (aae) an

90 Here, truncation of P65 due to transposon insertion in the corresponding gene resulted

91 1 mutant (bm1-das1) that contains a 3,444-bp transposon insertion in the first intron of CAD2 gene.

92 A transposon insertion in the middle of the coding sequenc

93 of maize knotted1, Kn1-DL, which contains a transposon insertion in the promoter in addition to a ta

94 mutagenesis-defective mutants, we isolated a transposon insertion in the rpoE P2 promoter.

95 a mutant M. tuberculosis strain containing a transposon insertion in the Rv3615c gene, which is situa

96 ted expression potentially associated with a transposon insertion in the upstream intergenic region,

97 genetic variation including a Harbinger DNA transposon insertion in the upstream regulatory region o

98 of these transformants were shown to have a transposon insertion in the uspA1 gene.

99 In this study, we report that a transposon insertion in the waaL gene, encoding O-antige

100 solines, as well as the presence of a 5.7-kb transposon insertion in the wp mutant allele, have unequ

101 Here we report that a Mu transposon insertion in the Zea mays (maize) gene encodi

102 Our lab recently isolated a transposon insertion in waaL, encoding O-antigen ligase,

103 utants allowed us to monitor the behavior of transposon insertions in 758 different gene loci.

104 servation and our ability to generate random transposon insertions in A. actinomycetemcomitans, we de

105 sequencing identified more than 200 de novo transposon insertions in alphabeta neurons, including in

106 M. truncatula lines harboring transposon insertions in CCR1 exhibit drastically reduce

107 Using transposon insertions in cloned katA DNA, we found that

108 However, transposon insertions in either rpoN, truB, PA4068, PA40

109 Transposon insertions in gacA and gacS increased sliding

110 We describe phenotypes conditioned by transposon insertions in genes encoding the maize (Zea m

111 g technique whereby those mutants possessing transposon insertions in genes essential for in vivo sur

112 Three mutants, each of which harboured transposon insertions in genes for transmembrane protein

113 identified competence-defective mutants with transposon insertions in genes not previously implicated

114 ses with Drosophila strains carrying P[lacW] transposon insertions in genes without documented recomb

115 Transposon insertions in Geobacter sulfurreducens GSU150

116 Four mutants contained transposon insertions in gerHA, gerHB, gerHC, and pagA,

117 Many of the pgl mutants are produced by transposon insertions in glycosyltransferase genes.

118 characterize gliding-altered mutants having transposon insertions in MPN311, encoding the cytoskelet

119 we identified thousands of putative somatic transposon insertions in neurons from individual Drosoph

120 developed mammary tumors, most of which had transposon insertions in one of two RASGAP genes, neurof

121 to RNI resistance, we isolated mutants with transposon insertions in pafB and pafC.

122 Four of the mutants had transposon insertions in remA, which encodes a cell surf

123 ndril formation to spreading colonies, while transposon insertions in retS abolished motility.

124 acid-sensitive M. tuberculosis mutants with transposon insertions in Rv2136c, Rv2224c, ponA2, and ly

125 Transposon insertions in sprB resulted in cells that wer

126 Transposon insertions in the 269 growth-defective strain

127 Interestingly, transposon insertions in the epidermal growth factor rec

128 Transposon insertions in the only annotated Ser/Thr prot

129 Transposon insertions in the oprD gene led not only to c

130 o reactive nitrogen intermediates identified transposon insertions in the presumptive proteasomal ATP

131 ts in the H37Rv strain background containing transposon insertions in the rv0072, rv0405, and rv2958c

132 Three of these mutants had transposon insertions in the uspA2H gene, which encodes

133 l-dependent endopeptidase, acquired distinct transposon insertions in two independent mutants.

134 Mutant strains harbouring transposon insertions in two such genes, toxR (a toxin r

135 tectable in bacteria carrying an hpf::Himar1 transposon insertion, indicating that HPF is required fo

136 ct on specialized metabolite production of a transposon insertion into a Pseudomonas aeruginosa phena

137 unctiforme ATCC 29133 was obtained by random transposon insertion into open reading frame NpR1273.

138 oson mutant library, we showed that a single transposon insertion into the A. phagocytophilum dihydro

139 Rather, Tn5367 transposon insertion into the prrA promoter only decreas

140 Transposon insertions into genes encoding functions nece

141 plant genome sizes are largely explained by transposon insertions into heterochromatic regions.

142 teins are endowed with the ability to direct transposon insertions into the genome near to where they

143 The FT2c allele carrying a transposon insertion is nearly fixed in soybean landrace

144 encing approaches applied to the analyses of transposon insertion junction fragments generated in hig

145 le-cell WGA method, Linear Amplification via Transposon Insertion (LIANTI), which outperforms existin

146 genes through construction of complex random transposon insertion libraries and quantification of eac

147 Random transposon insertion libraries have proven invaluable in

148 sequencing affords an efficient analysis of transposon insertion libraries, which can be used to ide

149 hia coli, we used flow cytometry to screen a transposon insertion library and identified a wecE mutan

150 Using a highly saturated transposon insertion library combined with next-generati

151 o identify regulators of CyaB, we screened a transposon insertion library for mutants with reduced in

152 Phenotypic screening of a GBS transposon insertion library identified a mutation withi

153 However, our analyses of the high-density transposon insertion library in pESBL also revealed two

154 We constructed a 2,950-member Tn917 transposon insertion library in S. aureus strain NCTC 83

155 ly test this hypothesis, we used a bar-coded transposon insertion library in tandem with cell sorting

156 Here, we present a near-saturating transposon insertion library in Vibrio cholerae strain C

157 Screening of a transposon insertion library of A. baumannii ATCC 19606T

158 By screening a transposon insertion library of F. tularensis LVS in the

159 etter understand this process, we screened a transposon insertion library of the F. tularensis live v

160 and the genetics of this process, a mini-Tn5 transposon insertion library was constructed in strain E

161 A high-density transposon insertion library was grown under biofilm-ind

162 omparison of the genes disrupted in the PA14 transposon insertion library with an independently const

163 based on the assembly of a saturated Mariner transposon insertion library.

164 We have used a transposon insertion line to investigate the physiologic

165 Loss of function of CSE in transposon insertion lines of M. truncatula results in s

166 A large-scale mutant screen of Ds transposon insertion lines was employed to identify 130

167 isolated from a population of Arabidopsis Ds transposon insertion lines.

168 ity defects were characterized further, with transposon insertions mapping to 32 different open readi

169 The paternal disruption of imprinting by transposon insertions may reflect a requirement for sequ

170 medulloblastoma dissemination have come from transposon insertion mutagenesis studies.

171 a catarrhalis ETSU-9 was subjected to random transposon insertion mutagenesis to identify genes encod

172 In this work, a transposon insertion mutant (cpsA::Tn) of Mycobacterium

173 , we report the isolation of a P. gingivalis transposon insertion mutant altered in biofilm developme

174 tely annotate the extremely large progenitor transposon insertion mutant collections needed to achiev

175 A transposon insertion mutant has been identified in a Des

176 A chlamydial transposon insertion mutant in the Cdu1-encoding gene ex

177 In previous work, we identified a transposon insertion mutant in the FTT0107c locus that w

178 Exposing a saturating transposon insertion mutant library of S Typhimurium to

179 screening method to identify mutants from a transposon insertion mutant library which exhibited dist

180 measure the exometabolome of 86 single-gene transposon insertion mutant strains (mutants from centra

181 tularensis subsp. tularensis strain Schu S4 transposon insertion mutant with a mutation in a predict

182 study, we describe a Drosophila melanogaster transposon insertion mutant with tolerance to Vibrio cho

183 ised of approximately 31,500 Y. pestis KIM6+ transposon insertion mutants (input pool) was subjected

184 Certain in-frame transposon insertion mutants did not interact with DNA a

185 by which this regulation occurs, we screened transposon insertion mutants for those that could migrat

186 iation, we searched a library of V. fischeri transposon insertion mutants for those that failed to co

187 ed the relative fitness of 41,000 Salmonella transposon insertion mutants growing in mouse models of

188 prehensive library of 2,998 sequence-defined transposon insertion mutants in Francisella novicida str

189 Screening of approximately 3,000 transposon insertion mutants in the crystal violet-based

190 involved in K(+)-dependent colony spreading, transposon insertion mutants in wild-type strain 3610 we

191 A total of 4,330 transposon insertion mutants of an invasive G1 Nal(r) st

192 the GlpT homologs in F. novicida and tested transposon insertion mutants of glpT.

193 e cytotoxicity and biofilm formation from 93 transposon insertion mutants previously reported with in

194 Two of the other three M. catarrhalis ETSU-9 transposon insertion mutants that had greatly reduced ab

195 genetic screen to identify L. monocytogenes transposon insertion mutants that induced altered levels

196 We screened E. coli transposon insertion mutants to look for proteins that m

197 The parental library of PA14 transposon insertion mutants was generated by using MAR2

198 Transposon insertion mutants were obtained with defects

199 Enterococcus faecalis transposon insertion mutants were screened for attenuate

200 ng a colony immunoblot screen, we identified transposon insertion mutants which were deficient for ty

201 Random screening of Y. pseudotuberculosis transposon insertion mutants with a C. elegans biofilm a

202 tors involved in these processes, DC3000 Tn5 transposon insertion mutants with reduced virulence on A

203 Examining a library of transposon insertion mutants, we identified the LysR-typ

204 i was identified from a collection of random transposon insertion mutants.

205 We found that transposon-insertion mutants for 46% of the essential ge

206 A transposon insertion mutation in pgant3 or RNA interfere

207 stress defense, we isolated a mutant with a transposon insertion mutation of sitA, which encodes the

208 Transposon insertion mutations in Gemin3 are larval leth

209 ive mutants led to the identification of 174 transposon insertion mutations that mapped to 13 individ

210 To identify genes essential for A motility, transposon insertion mutations with defective A motility

211 e functions from the phenotypes arising from transposon insertion mutations.

212 Finally, we show novel transposon insertions negatively correlate with Piwi-int

213 transport-defective mutant revealed that the transposon insertion occurred in an open reading frame (

214 In a second mutant, the transposon insertion occurred in mrpB, which is part of

215 ased in a transposon mutant (NBC-28G9) where transposon insertion occurred in the 5' end of gidA gene

216 e show that we can determine the effect of a transposon insertion on its target gene(s) and prioritiz

217 Gene inactivation by transposon insertion or allelic exchange is a powerful a

218 (gTME), and gene-disruption methods such as transposon insertion or site-specific homologous recombi

219 h on the basis of the assumption that recent transposon insertions or excisions create singleton or l

220 t model of endocarditis to test strains with transposon insertions or in-frame deletions in biofilm-a

221 ction was used to explain observed miniHimar transposon insertion patterns, and gene essentiality cal

222 psis containing T-DNA insertion (pbs3-2) and transposon insertion (pbs3-3) mutations in At5g13320 con

223 To identify transposon insertion points, a unique primer directed ou

224 Because we identified all transposon insertion polymorphisms with a single method,

225 We perform transposon insertion profiling by microarray (TIP-chip)

226 insertions selectively in the human genome, transposon insertion profiling by next-generation sequen

227 Large-scale transposon insertion projects provide excellent material

228 tatistically significant number of recurrent transposon insertions, respectively.

229 n of this mutation by genetic removal of the transposon insertion restored MAb 2-1-L8 binding.

230 Here, we analyzed transposon-insertion rice lines disrupted in OsHKT2;1.

231 A transposon insertion screen implicated the yejH gene in

232 Transposon insertion sequencing (Tn-Seq) is a microbial

233 Transposon insertion sequencing (Tn-seq) is an emerging

234 Transposon insertion sequencing is a high-throughput tec

235 Here, a transposon insertion sequencing technology called INSeq

236 s for V. cholerae survival, and by combining transposon-insertion sequencing and transcriptomic data

237 We have refined analysis of transposon-insertion sequencing data by normalizing for

238 re unappreciated information inherent in all transposon-insertion sequencing data sets.

239 nhance extraction of biological meaning from transposon-insertion sequencing genomic data.

240 We used transposon-insertion sequencing to screen for genes that

241 tagenesis to high-throughput DNA sequencing (transposon-insertion sequencing) enables simultaneous an

242 Here, we used a transposon insertion site (TIS) sequencing-based strateg

243 After determining the transposon insertion site for each mutant, unique revers

244 Using PCR, we identified a transposon insertion site in the first intron of Nmnat2

245 Jak2 was identified as a common transposon insertion site in TLS-ERG-induced disease, st

246 The transposon insertion site of this mutant strain was with

247 We have designed a transposon insertion site profiling chip (TIP-chip), a m

248 tion sequencing allows affordable ultra-deep transposon insertion site recovery in high-throughput fo

249 -mediated tethering can effectively redirect transposon insertion site selection in human cells, but

250 also describe a method for semiquantitative transposon insertion site sequencing (QiSeq).

251 We used transposon insertion site sequencing (Tn-seq) to compreh

252 Here, we carried out a high-throughput, transposon insertion site sequencing analysis (TnSeq) to

253 The transposon insertion site was identified for 29 of the 1

254 Transposon insertion-site sequencing was used to analyze

255 quence reads mapped to millions of potential transposon insertion sites and a large portion of insert

256 In the analysis of these screens, transposon insertion sites are typically identified by t

257 To determine whether genes located at transposon insertion sites directly caused medulloblasto

258 g (SBCapSeq), that facilitates sequencing of transposon insertion sites from single tumor cells in a

259 Cloning transposon insertion sites from these tumours revealed t

260 Sequence analysis of transposon insertion sites from tumors and immortalized

261 Isolation of the transposon insertion sites identified genes known to be

262 Analysis of transposon insertion sites identified novel candidate ge

263 After sequencing transposon insertion sites in 9,250 random mutants, we a

264 Characterization of transposon insertion sites in the SB-induced tumors iden

265 ficient methods were established to identify transposon insertion sites in these lesions, and analysi

266 n pathways were conspicuous among the common transposon insertion sites in TLS-ERG-driven leukemia, s

267 Common transposon insertion sites occur among haplotypes from d

268 alyze approximately 2 x 10(5) unique de novo transposon insertion sites of the transposon Hermes in t

269 ion of direct, high-throughput sequencing of transposon insertion sites revealed fitness phenotypes o

270 induced astrocytoma tissue was extracted and transposon insertion sites were identified.

271 90 BACs and 165 cDNA's, as well as 69 genes, transposon insertion sites, sequence-tagged sites, micro

272 sensitive and robust system for identifying transposon insertion sites.

273 ive molecular framework for the interplay of transposon insertion, SNP/indel mutation, and epigenetic

274 We show that genetic complementation of the transposon insertion strain partially restored the trans

275 ion was attributed to a natural CACTA family transposon insertion that inactivates Bx10c in maize lin

276 divergence between maize and teosinte, and a transposon insertion that inactivates Bx12 was under str

277 Three mucoid mutants were identified with transposon insertions that caused 1) an overexpression o

278 also close to the locations of several gypsy transposon insertions that disrupt Ubx expression, leadi

279 Finally, we discovered a large set of transposon insertions that trigger premature initiation

280 modern species, such as remnants of ancient transposon insertions, that were not identified by direc

281 tion to characterize genomic regions lacking transposon insertions, this method is capable of identif

282 oughput DNA sequencing, screened genome-wide transposon insertion (Tn-seq) and transcriptome (RNA-seq

283 struct appropriate statistical tests for the transposon insertion tolerance of normal genes of intere

284 Transposon insertions typically resulted in altered expr

285 Transposon insertions upstream of csgC and lrhA or withi

286 A library of over 41,000 unique transposon insertions was analyzed before and after colo

287 P-mediated recombination between FRT-bearing transposon insertions was used to generate deletions, be

288 s of D. melanogaster, carrying single P[GT1] transposon insertions, we found 267 lines that showed si

289 llisepticum genome, the precise locations of transposon insertions were discerned.

290 Recurrent and mutually exclusive transposon insertions were identified in Myh9, Ppp1r12a,

291 nsertions (0.86%) were biofilm negative, and transposon insertions were located in 51 distinct genes/

292 Single-copy transposon insertions were transposed into the rat genom

293 in exons (8%) may deter insertion of Mutator transposon insertion, while CHG methylation at splice ac

294 A new collection of Minos transposon insertions will enhance the range and flexibi

295 One of these mutants possessed a transposon insertion within a gene, designated mrkI, enc

296 psis line containing a knock-out dissociator transposon insertion within the single copy AtPLMT gene

297 Transposon insertions within genes for sulfate assimilat

298 ered a set of genetic suppressors harbouring transposon insertions within genes of a locus encoding A

299 The maternal disruption of imprinting by transposon insertions within the Mez1 promoter suggests

300 Three novel Mez1 alleles containing Mutator transposon insertions within the promoter were identifie