ライフサイエンスコーパス: transposon mutagenesis

1 avily mutagenized individuals (site-selected transposon mutagenesis).

2 cid UL25 open reading frame was disrupted by transposon mutagenesis.

3 rtion mutants were created for both genes by transposon mutagenesis.

4 ted from a serotype C strain 26404 by random transposon mutagenesis.

5 ream of the pilin gene pilE was targeted for transposon mutagenesis.

6 le to form biofilms in vitro and amenable to transposon mutagenesis.

7 R-A specific deletion mutants isolated using transposon mutagenesis.

8 ial protein that could not be uncovered with transposon mutagenesis.

9 tant libraries can be readily constructed by transposon mutagenesis.

10 he strategy of differential signature-tagged transposon mutagenesis.

11 rus AD169 genome by random and site-directed transposon mutagenesis.

12 n ftfL homolog identified previously through transposon mutagenesis.

13 useful for reverse genetics than for forward transposon mutagenesis.

14 entation system in Escherichia coli based on transposon mutagenesis.

15 s) were identified, and each was analyzed by transposon mutagenesis.

16 pacX was cloned by impala transposon mutagenesis.

17 iviparous 14 (vp14) was recently obtained by transposon mutagenesis.

18 ants of M. xanthus were isolated through Tn5 transposon mutagenesis.

19 sion (CCR) of the hut operon was isolated by transposon mutagenesis.

20 y osmotica were sought by a further round of transposon mutagenesis.

21 ant, viviparous14 (vp14), were identified by transposon mutagenesis.

22 ctive for macrophage killing was isolated by transposon mutagenesis.

23 re derived from wild-type strain AA100 after transposon mutagenesis.

24 cobacterium smegmatis was isolated following transposon mutagenesis.

25 F gene was identified by random miniTn10-tet transposon mutagenesis.

26 ne utilization (hut) operon were isolated by transposon mutagenesis.

27 ants of these strains derived by chemical or transposon mutagenesis.

28 tor of Y. pestis, we performed mariner-based transposon mutagenesis.

29 a forward genetic screen using gene-breaking transposon mutagenesis.

30 In global transposon mutagenesis, 173 insertion mutants out of 15,

31 Previously, we had isolated by transposon mutagenesis a Legionella pneumophila mutant t

32 Using transposon mutagenesis, a gene, designated hmpF, was ide

33 Transposon mutagenesis, allelic replacement, and electro

34 After mini-mariner transposon mutagenesis, an SDS-resistant suppressor muta

35 Transposon mutagenesis and a visual screen were used to

36 The use of a combination of transposon mutagenesis and arginine hydroxamate selectio

37 gene encoding this protein was identified by transposon mutagenesis and characterized.

38 an macrophages was generated by Tn903dIIlacZ transposon mutagenesis and classified into DNA hybridiza

39 Transposon mutagenesis and comparative sequence analysis

40 ipid A portion of the LOS, was identified by transposon mutagenesis and construction of an isogenic k

41 e we report a high-throughput approach using transposon mutagenesis and deep sequencing (Tn-seq) to i

42 ve whole-genome analysis of gene function by transposon mutagenesis and deep sequencing methodology h

43 Transposon mutagenesis and genetic studies revealed that

44 ed a deletion allele of ERG by site-selected transposon mutagenesis and have shown that seeds contain

45 motes commensalism, we used signature-tagged transposon mutagenesis and identified 29 mutants represe

46 We used genome-wide transposon mutagenesis and insertion-site sequencing, RN

47 ction" technology that combines high-density transposon mutagenesis and massively parallel sequencing

48 construction, screening approaches combining transposon mutagenesis and microarray technology, and th

49 operon deletion mutant was subjected to Tn5 transposon mutagenesis and new mutants selected using a

50 tifying individually deletable regions using transposon mutagenesis and progressively clustering dele

51 a forward-genetic procedure involving random transposon mutagenesis and rapid phenotypic screening, w

52 We performed a transposon mutagenesis and screened for mutants with alt

53 Transposon mutagenesis and screening for increased beta-

54 n, we subjected a dnaA(cos) mutant strain to transposon mutagenesis and selected mutant derivatives t

55 Using near-saturation transposon mutagenesis and testing of transposon-tagged

56 Subcloning, transposon mutagenesis, and DNA sequence analysis reveal

57 nsposon insertions, stringent suppression of transposon mutagenesis, and few polar effects.

58 nt alleles of the DIF1 gene were isolated by transposon mutagenesis, and the mutants show complete ma

59 The irrE locus of R1 was also inactivated by transposon mutagenesis, and this strain was sensitive to

60 of-principle for the utility of the low-copy transposon mutagenesis approach for identifying cancer-d

61 Using a Tn3-based transposon mutagenesis approach, we have generated a poo

62 Using a transposon mutagenesis approach, we have identified a mu

63 In this study, using a powerful transposon mutagenesis approach, we have identified in S

64 Finally, using a transposon mutagenesis approach, we identified a new pos

65 as previously generated by using a Tn3-based transposon mutagenesis approach.

66 alovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach.

67 alovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach.

68 lr4311, and all4388, identified initially by transposon mutagenesis, are such genes by complementing

69 The ymoBA locus was identified by transposon mutagenesis as a repressor of inv expression

70 Mini-Tn10kan transposon mutagenesis at different sites within the ope

71 Using transposon mutagenesis, chromosomal walking steps, and d

72 Whole genome genetic fitness analysis using transposon mutagenesis combined with next-generation hig

73 One locus identified by transposon mutagenesis conferred protection against heme

74 charomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequen

75 Transposon mutagenesis, coupled with a novel reporter sy

76 e of molecular biology combined with limited transposon mutagenesis data, failed to produce a viable

77 d be identified in the same parent strain by transposon mutagenesis, despite extensive searches.

78 A pilot-scale transposon mutagenesis experiment using a modified auton

79 ompared with data obtained from recent dense-transposon mutagenesis experiments.

80 o screen isogenic mutants generated by Tn916 transposon mutagenesis for defective biofilm formation.

81 Transposon mutagenesis has thus provided a better unders

82 Transposon mutagenesis identified a LysR-like regulator,

83 T-POP transposon mutagenesis identified marT as a positive reg

84 Random transposon mutagenesis identified null mutations and ove

85 Transposon mutagenesis identified several gene products

86 Transposon mutagenesis identified two monooxygenases, Ab

87 Transposon mutagenesis implicated lipopolysaccharide (LP

88 Transposon mutagenesis in bacteria generally requires ef

89 ghly transcribed operons were insulated from transposon mutagenesis in both organisms.

90 Here, using random transposon mutagenesis in conjunction with in vitro mode

91 al galactose-sensitive adhesin, a system for transposon mutagenesis in fusobacteria was created.

92 scribe an efficient system for site-selected transposon mutagenesis in maize.

93 Using transposon mutagenesis in mice many laboratories have co

94 odules to rapidly generate tagged mutants by transposon mutagenesis in the metal-reducing bacterium S

95 ates, and is therefore a valuable vector for transposon mutagenesis in vertebrate models and for huma

96 a number of useful derivatives suitable for transposon mutagenesis in vivo or in vitro.

97 Transposon mutagenesis, in combination with parallel seq

98 Results from subcloning and transposon mutagenesis indicated that the complete 6.7-k

99 Transposon mutagenesis indicated that the two complement

100 A second drl2 allele, produced by transposon mutagenesis, interacted synergistically with

101 mbination of high-throughput sequencing with transposon mutagenesis is a powerful approach for the id

102 High-copy transposon mutagenesis is an effective tool for creating

103 Mitomycin C-sensitive clones from a transposon mutagenesis library were screened.

104 Using transposon mutagenesis, lrs mutants were found in 11 dif

105 ejuni have been limited due to the lack of a transposon mutagenesis method.

106 inally, we directly compared T-DNA and Ac/Ds transposon mutagenesis methods in Arabidopsis on a genom

107 Improved transposon mutagenesis methods revealed a class of quasi

108 2), we have employed high-throughput shuttle transposon mutagenesis of a cosmid library prepared in E

109 Transposon mutagenesis of a Deltahha esp::lac strain exp

110 We present here a method for in vivo transposon mutagenesis of a methanogenic archaeon, Metha

111 Transposon mutagenesis of a strain carrying a deletion s

112 ate the genetic controls governing swarming, transposon mutagenesis of a TR (DeltaopaR1) strain was p

113 We performed transposon mutagenesis of a two-color fluorescent report

114 Transposon mutagenesis of A. oris yielded a mutant defec

115 Transposon mutagenesis of Agrobacterium tumefaciens iden

116 Transposon mutagenesis of an encapsulated, virulent stra

117 e high-throughput process relies on in vitro transposon mutagenesis of an ordered cosmid library; mut

118 In this study, transposon mutagenesis of an unencapsulated strain yield

119 Transposon mutagenesis of Bordetella pertussis was used

120 nstrated, for the first time, random in vivo transposon mutagenesis of C. jejuni.

121 By conducting transposon mutagenesis of exoK mutants of R. meliloti an

122 Through transposon mutagenesis of F. tularensis subsp. holarctic

123 e results of this study indicate that random transposon mutagenesis of infectious B. burgdorferi is f

124 We combined high-coverage transposon mutagenesis of influenza virus with a rapid h

125 ata demonstrate the utility of mariner-based transposon mutagenesis of M. marinum and that M. marinum

126 Transposon mutagenesis of Mycobacterium tuberculosis has

127 rol of AlgB which affect algD transcription, transposon mutagenesis of nonmucoid algB derivatives of

128 Furthermore, transposon mutagenesis of oxa-23 or interactors of Oxa-2

129 Transposon mutagenesis of P. aeruginosa 388 was used to

130 ine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1.

131 Here, we have used random transposon mutagenesis of R. rickettsii to generate a sm

132 Signature-tagged transposon mutagenesis of Salmonella with differential r

133 Transposon mutagenesis of SBW25DeltafleQ (SBW25Q) produc

134 volved in swarming, we carried out extensive transposon mutagenesis of serovar Typhimurium, screening

135 In this study we have undertaken transposon mutagenesis of SH1000 to identify components

136 Transposon mutagenesis of strain AW1-PC (phcA1) generate

137 Transposon mutagenesis of Synechococcus elongatus PCC 79

138 for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4.

139 . marinum model, we have developed efficient transposon mutagenesis of the organism by using a Drosop

140 Transposon mutagenesis of the rugose variant led to the

141 Transposon mutagenesis of this plasmid identified the po

142 tone carboxylase subunits were identified by transposon mutagenesis of X. autotrophicus and sequence

143 Transposon mutagenesis of X. autotrophicus revealed a re

144 Here, we report the isolation, by transposon mutagenesis, of tat mutant strains that have

145 , we describe a simple system for performing transposon mutagenesis on naturally transformable organi

146 tants were identified from a second round of transposon mutagenesis performed in an mtr efflux pump-d

147 ) mutants was generated by using a Tn3-based transposon mutagenesis procedure.

148 ess as a generic tool supporting genome-wide transposon mutagenesis programs, these data provide insi

149 Transposon mutagenesis provides a direct selection for m

150 Transposon mutagenesis provides a useful tool for ricket

151 ompletion of the trypanosome genome project, transposon mutagenesis provides an important addition to

152 Whole genome transposon mutagenesis revealed a 19-gene locus, involve

153 Subcloning and transposon mutagenesis revealed a 5.6-kb region, designa

154 Random transposon mutagenesis revealed that disruption of rsmA

155 A transposon mutagenesis screen for additional regulators

156 Our previous transposon mutagenesis screen identified 95 lung infecti

157 Finally, a magellan-4 transposon mutagenesis screen identified glcK, a putativ

158 driving HCC, we performed a near-saturating transposon mutagenesis screen in a mouse HBV model of HC

159 genetic forces driving TNBC, we performed a transposon mutagenesis screen in a phosphatase and tensi

160 er genes, we carried out a low-copy piggyBac transposon mutagenesis screen in mice.

161 s hypothesis by performing a Sleeping Beauty transposon mutagenesis screen in which common insertion

162 The Sleeping Beauty (SB) transposon mutagenesis screen is a powerful tool to faci

163 Following a global transposon mutagenesis screen of EAEC 042, the transcrip

164 Transposon mutagenesis screen of meningitis-causing E. c

165 A transposon mutagenesis screen revealed additional compon

166 reporter system to facilitate a large-scale transposon mutagenesis screen to identify genes required

167 We used it to perform a Sleeping Beauty transposon mutagenesis screen to identify genes that coo

168 We have conducted a transposon mutagenesis screen to identify mutants defici

169 We used a genome-wide transposon mutagenesis screen to identify where mutation

170 Here, we describe a genome-wide transposon mutagenesis screen to isolate recipient mutan

171 siological signals affecting this pathway, a transposon mutagenesis screen was carried out to find fa

172 A transposon mutagenesis screen was performed with the rep

173 In a transposon mutagenesis screen, three transfer-defective

174 Through an extensive transposon mutagenesis screen, we have identified severa

175 During a transposon mutagenesis screen, we identified a novel gen

176 pyogenes mutant, SalY, was identified from a transposon mutagenesis screen, with over 200-fold attenu

177 terium extorquens AM1 were identified from a transposon mutagenesis screen.

178 ncogene in nonmelanoma skin cancer through a transposon mutagenesis screen.

179 s required for transfer, we have performed a transposon mutagenesis screen; we report here that LpqM,

180 Signature-tag transposon mutagenesis screening of a 1520-member librar

181 In this study, using a transposon mutagenesis screening, we identified a cell s

182 ogression, we performed Sleeping Beauty (SB) transposon mutagenesis screens in mice carrying sensitiz

183 nally organized, we carried out whole-genome transposon mutagenesis screens in Mtb strains deleted fo

184 critical for Yop delivery, we initiated two transposon mutagenesis screens using the mariner transpo

185 We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomo

186 ains were derived by three distinct methods: transposon mutagenesis, serial passage in the presence o

187 Transposon mutagenesis showed that a P. aeruginosa mutan

188 ms in this system proved to be refractory to transposon mutagenesis, so an M. catarrhalis strain was

189 In this study, we use signature-tagged transposon mutagenesis (STM) to conduct a screen for ran

190 ative selection strategy of signature-tagged transposon mutagenesis (STM) to screen mutants for loss

191 In this study, signature-tagged transposon mutagenesis (STM) was used to identify genes

192 In this study, we used a transposon mutagenesis strategy based on a two-step Slee

193 Using a unique transposon mutagenesis strategy that mutagenizes NSCs in

194 A high-density transposon mutagenesis strategy was applied to the Haemo

195 ts were created in E. coli using an in vitro transposon mutagenesis strategy.

196 Transposon mutagenesis studies strongly suggest that BAP

197 Previous transposon mutagenesis studies with this organism were b

198 sional gel electrophoresis, and whole-genome transposon mutagenesis studies.

199 n regulation of this two-component system, a transposon mutagenesis study was designed to identify su

200 Transposon mutagenesis suggested three possible targets:

201 ut transient targeted mutagenesis and random transposon mutagenesis suggests that stable targeted kno

202 pping techniques, including fragmentation by transposon mutagenesis, synthetic peptides, phage-displa

203 Here we describe an in vitro transposon mutagenesis system based on the Staphylococcu

204 We have developed an IS903-based transposon mutagenesis system for diverse gram-negative

205 Here we report on the development of a transposon mutagenesis system for use with marine Synech

206 Using the candystripe1 transposon mutagenesis system in sorghum, we have isolat

207 The Sleeping Beauty (SB) transposon mutagenesis system is a powerful tool that fa

208 ard that end, we have developed an efficient transposon mutagenesis system that makes use of a Himar1

209 liding, we developed a plasmid-based mariner transposon mutagenesis system that works effectively in

210 A novel transposon mutagenesis system was established and used t

211 A random in vivo transposon mutagenesis system was recently developed for

212 The in vitro transposon mutagenesis technique was shown to be a power

213 saSBase(LsL), that allows the restriction of transposon mutagenesis to a specific tissue of interest.

214 nvestigate ZIKV genetic flexibility, we used transposon mutagenesis to add 15-nucleotide insertions t

215 ode hypothetical proteins were determined by transposon mutagenesis to be required for optimal photoa

216 ated from an ongoing study using large-scale transposon mutagenesis to characterize gene function in

217 A new study uses Sleeping Beauty transposon mutagenesis to drive osteosarcomagenesis in t

218 comprehensive collection of mutants, we used transposon mutagenesis to generate a large collection of

219 We have used transposon mutagenesis to generate a library of PLD1 all

220 f a supercoiling-sensitive promoter and used transposon mutagenesis to generate a wide range of mutan

221 The coupling of high-density transposon mutagenesis to high-throughput DNA sequencing

222 In this study, we used random transposon mutagenesis to identify a 3-deoxy-D-manno-oct

223 We used Sleeping Beauty (SB) transposon mutagenesis to identify events that cooperate

224 Using Tn5-transposon mutagenesis to identify factors that upend le

225 We used transposon mutagenesis to identify genes responsible for

226 We used random transposon mutagenesis to identify genes that are requir

227 study, we examined the feasibility of using transposon mutagenesis to identify infectivity-related f

228 , we developed a genetic selection that uses transposon mutagenesis to identify loci affecting the ae

229 se findings demonstrate the utility of using transposon mutagenesis to identify low-frequency and coo

230 Herein, we used random transposon mutagenesis to identify LVS genes that affect

231 We used random transposon mutagenesis to identify new genes involved in

232 We used transposon mutagenesis to identify S. Enteritidis virule

233 We report the use of transposon mutagenesis to identify two genes required fo

234 We used transposon mutagenesis to investigate whether other gene

235 we take advantage of haploinsufficiency and transposon mutagenesis to perform large-scale loss-of-fu

236 We performed an ISS1 transposon mutagenesis to screen for acid-sensitive muta

237 We developed a strategy for high-efficiency transposon mutagenesis to screen for genes in B. hensela

238 Using signature-tagged transposon mutagenesis to screen for novel virulence fac

239 targeted cre-dependent Sleeping Beauty (SB) transposon mutagenesis to the blood-forming system using

240 As a result of our recent development of a transposon-mutagenesis tool that overcomes the barrier t

241 Using transposon mutagenesis, two mutant strains were identifi

242 nvestigations of hemA regulation, we applied transposon mutagenesis under aerobic conditions, followe

243 ve developed an efficient system of in vitro transposon mutagenesis using a mariner-based transposon

244 re, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon

245 bank consisting of 11,354 mutants by mariner transposon mutagenesis using Brucella melitensis rough m

246 Transposon mutagenesis was conducted to identify inserti

247 rganisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants

248 Random T-POP transposon mutagenesis was employed to screen for insert

249 SB transposon mutagenesis was performed in either a wild-ty

250 Saturating transposon mutagenesis was performed in wild type and a

251 s regulation of motility by type 1 fimbriae, transposon mutagenesis was performed on a phase-locked t

252 fy genes necessary for bile resistance, MudJ transposon mutagenesis was performed on a strain contain

253 tify factors that regulate toxin expression, transposon mutagenesis was performed under non-inducing

254 sign functions of the various ORFs, in vitro transposon mutagenesis was performed using the cosmid DN

255 A benR mutant isolated by random transposon mutagenesis was unable to grow on benzoate.

256 of CDT in the pathogenesis of H. hepaticus, transposon mutagenesis was used to generate a series of

257 Random transposon mutagenesis was used to generate A. baumannii

258 role of O antigen in virulence and survival, transposon mutagenesis was used to generate B. abortus r

259 Transposon mutagenesis was used to generate mutants of S

260 Transposon mutagenesis was used to identify an open read

261 Transposon mutagenesis was used to identify loci which n

262 Transposon mutagenesis was used to identify new genes th

263 Global transposon mutagenesis was used to identify nonessential

264 Random MudJ transposon mutagenesis was used to identify PmrA-PmrB-re

265 In this study, transposon mutagenesis was used to identify possible com

266 Transposon mutagenesis was used to identify regulators o

267 Transposon mutagenesis was used to identify sprE, which

268 Saturating transposon mutagenesis was used to identify the loci res

269 In the present study, TnphoA transposon mutagenesis was used to identify the TNF-alph

270 Transposon mutagenesis was used to identify transcriptio

271 In this study, signature-tagged transposon mutagenesis was used to identify Yersinia ent

272 Transposon mutagenesis was used to locate genes involved

273 Transposon mutagenesis was used to obtain mutations affe

274 ophs of Mycobacterium bovis BCG, obtained by transposon mutagenesis, was used to select methionine au

275 Using transposon mutagenesis we have identified a genetic locu

276 Using random transposon mutagenesis, we determined that the phenotype

277 -mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of th

278 Using transposon mutagenesis, we have identified several regul

279 Using transposon mutagenesis, we identified 14 novel dynamic a

280 Using transposon mutagenesis, we identified a stand-alone poly

281 Using transposon mutagenesis, we identified factors that contr

282 Using transposon mutagenesis, we identified surface cell antig

283 iniBAC induction in Mycobacterium marinum By transposon mutagenesis, we identified that the operon is

284 protein depletion system in combination with transposon mutagenesis, we identify mutants of P. aerugi

285 Using transposon mutagenesis, we isolated a noncytotoxic strai

286 Using global transposon mutagenesis, we isolated and characterized ge

287 ve in algI or algJ, obtained by chemical and transposon mutagenesis, were also defective in their abi

288 sed whole-genome fitness analysis, combining transposon mutagenesis with high-throughput sequencing,

289 seq) is an emerging technology that combines transposon mutagenesis with next-generation sequencing t

290 Here we report the use of transposon mutagenesis with the deep-sea bacterium Photo

291 ions in M. xanthus is extraordinarily broad, transposon mutagenesis with this eukaryotic genetic elem

292 Using transposon mutagenesis with Tn3HoHo1 and a binary transf

293 By using transposon mutagenesis with Tn3HoHo1, we localized the t

294 ae cytadherence was previously identified by transposon mutagenesis with Tn4001.

295 to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used.

296 We hypothesized that transposon mutagenesis would complement targeted knockou