ライフサイエンスコーパス: trifluoroethanol

1 rimethylamine N-oxide or the cosolvent 2,2,2-trifluoroethanol.

2 and a 1:1 (v/v) mixture of CH2Cl2 and 2,2,2-trifluoroethanol.

3 creased by 1.7 kcal/mol in a protic solvent, trifluoroethanol.

4 repared at neutral pH in the presence of 20% trifluoroethanol.

5 ximal I(SC) are predominantly helical in 40% trifluoroethanol.

6 elices under conditions of high salt, or 65% trifluoroethanol.

7 les, with 12% helicity induced in 50% v/v of trifluoroethanol.

8 alpha helix in the helix-stabilizing solvent trifluoroethanol.

9 ic spectra obtained in the presence of 2,2,2-trifluoroethanol.

10 rresponding imidazolium salts in basic 2,2,2-trifluoroethanol.

11 dency to adopt alpha-helical conformation in trifluoroethanol.

12 le reactivity toward the neutral nucleophile trifluoroethanol.

13 n in the presence of the hydrophobic solvent trifluoroethanol.

14 tion and aggregation propensity triggered by trifluoroethanol.

15 , melittin, in the presence of a denaturant, trifluoroethanol.

16 cis prolines, whereas none are detectable in trifluoroethanol.

17 idic pH and a cationic surfactant but not by trifluoroethanol.

18 ed S2-HR2) in the presence of the co-solvent trifluoroethanol.

19 acetonitrile, dimethyl sulfoxide, and 2,2,2-trifluoroethanol.

20 pha-helix in solution in the presence of 30% trifluoroethanol.

21 luding a zinc-coordinated substrate analogue trifluoroethanol.

22 s equilibrium between methanol (2(MeOD)) and trifluoroethanol (2(TFE)) adducts, with methanol binding

23 st, upon the addition of moderate amounts of trifluoroethanol (30%), the peptides adopted an alpha-he

24 8), 2-propanol (28 ps, KIE = 1.4), and 2,2,2-trifluoroethanol (4.4 ps, KIE = 3.2), which indicates th

25 In the presence of trifluoroethanol (50% [w/v]), the protein acquired a 30%

26 udied the unfolded ensemble populated in 50% trifluoroethanol, a denaturant that induces a highly hel

27 in aqueous solutions containing 40-80% 2,2,2-trifluoroethanol, a lipomimetic solvent, and was maximal

28 cally nonhelical and highly flexible even in trifluoroethanol, a solvent known to promote and stabili

29 lding, was dramatically accelerated by 2,2,2-trifluoroethanol, a solvent that stabilizes alpha-helica

30 the less nucleophilic solvent mixture of 1:9 trifluoroethanol:acetonitrile, with no formation of seco

31 High concentrations (>50%, v/v) of 2,2,2-trifluoroethanol also dissociate TTR(10-19) fibrils to t

32 ide, a folding inducing organic osmolyte, or trifluoroethanol, an alpha-helix inducer, alpha-helical

33 res for secondary structure, was modified in trifluoroethanol, an organic solvent that represents a m

34 complex with NAD and the substrate analogue trifluoroethanol and a 2.6 A complex with the isosteric

35 ponding silyl enol ethers were prepared from trifluoroethanol and chlorotrialkylsilanes in the presen

36 The fluorous alcohols trifluoroethanol and hexafluoro-2-propanol efficiently p

37 Trifluoroethanol and hexafluoroisopropanol were particul

38 ular Type-II 4 + 3 cycloaddition reaction in trifluoroethanol and hexafluoropropan-2-ol solvents, gen

39 tus PufX exhibited 59 and 55% alpha-helix in trifluoroethanol and in 0.80% octylglucoside in water, r

40 lyzed by circular dichroism in aqueous 2,2,2-trifluoroethanol and in buffered aqueous solutions, both

41 structures analysed, using 1H NMR and CD, in trifluoroethanol and in dodecylphosphocholine micelles.

42 tides were predominantly (40-80%) helical in trifluoroethanol and most trifluoroethanol-water mixture

43 c channel proteins were transferred to 2,2,2-trifluoroethanol and reconstituted into vesicle membrane

44 of nociceptin in membrane-like environments (trifluoroethanol and SDS micelles) and found it to have

45 The behaviour of the same peptides in trifluoroethanol and SDS solutions is consistent with fo

46 ar levels of alpha-helicity were observed in trifluoroethanol and the peptide appeared to adopt alpha

47 lices in the lower dielectric solvents 2,2,2-trifluoroethanol, and a 1:1 (v/v) mixture of CH2Cl2 and

48 ronsted acid proton source (water, methanol, trifluoroethanol, and phenol) that is required for this

49 d in acetonitrile solutions containing 2,2,2-trifluoroethanol, and several Arrhenius functions were d

50 in in solution in the presence of liposomes, trifluoroethanol, and sodium dodecyl sulfate indicated t

51 n aqueous solutions but partially helical in trifluoroethanol/aqueous and hexafluoroisopropanol/aqueo

52 solution structure of the C5 peptide in 40% trifluoroethanol/aqueous buffer was determined by NMR sp

53 onia as the amine component, employing 2,2,2-trifluoroethanol as a non-nucleophilic solvent in order

54 Use of trifluoroethanol as a solvent allowed for significant im

55 oltammetry studies show that both phenol and trifluoroethanol as proton sources exhibit the largest p

56 igh-resolution NMR structure of GlyR TM23 in trifluoroethanol as the starting template.

57 n alpha-helix content of 100% in 100% 2, 2,2-trifluoroethanol at 0 degrees C.

58 cetone, acetonitrile, ethanol, methanol, and trifluoroethanol at 0 degrees C.

59 cyclo[D-Asp(i),Dap(i+3)] derivatives in 80% trifluoroethanol at 25 and 5 degrees C suggested differe

60 peptides from TFE/H2O mixtures (TFE = 2,2, 2-trifluoroethanol) back to water, the thermal unfolding c

61 s, lipopolysaccharide (LPS) dispersions, and trifluoroethanol buffer systems.

62 ded towards an alpha-helical conformation in trifluoroethanol buffer, indicating that these regions a

63 es 12-18 for one peptide, CPY-Pl1, formed in trifluoroethanol buffer.

64 helical structure in the presence of SDS or trifluoroethanol but are predominantly unstructured in a

65 osylated wild type enzyme is reactive toward trifluoroethanol but not anions.

66 In contrast, trifluoroethanol caused dissociation of [MAL-6-alpha2]2

67 Trifluoroethanol challenge coupled with circular dichroi

68 The NAD-trifluoroethanol complex crystallizes in the closed conf

69 and properties of the 1:2 boron trifluoride-trifluoroethanol complex have been further studied using

70 of temperature, denaturant concentration and trifluoroethanol concentration.

71 protein structure, is observed at a critical trifluoroethanol concentration.

72 CD and NMR spectroscopies of the peptide in trifluoroethanol confirmed that hFSHR-(221-252) has the

73 1 is approximately 80% in 1-butanol or 2,2,2-trifluoroethanol, consistent with a previous membrane-fo

74 Inducing folding with trifluoroethanol cosolvent allows us to determine the fo

75 In the presence of trifluoroethanol, CsTx-1 and the long C-terminal part al

76 A amphipathic helical peptide in a 50% (v/v) trifluoroethanol-d3/water mixture, a membrane-mimic envi

77 y omission of the four data points for 2,2,2-trifluoroethanol-ethanol mixtures (F-test value from 155

78 ite its unstructured nature, the addition of trifluoroethanol exhibited an intrinsic potential in thi

79 The trifluoroethanol experiments suggest that the alpha-heli

80 A hydrophobic environment (trifluoroethanol) failed to induce alpha-helix formation

81 of the UV and circular dichroism spectrum in trifluoroethanol for compound 2 suggest that the putativ

82 Two modifiers (2,2,2-trifluoroethanol, formaldehyde) that produce anions with

83 SMAP-29 was flexible in 40% trifluoroethanol, forming two sets of conformers that di

84 2,2-dichloro- > 2-chloro approximately 2,2,2-trifluoroethanol > ethanol.

85 Reactions with pyrazole and trifluoroethanol had biphasic proton release, whereas re

86 c environments such as SDS micelles or water/trifluoroethanol, however, the peptide adopts a structur

87 2,2,2-Trifluoroethanol increased response of more hydrophobic

88 cture determined by NMR in 40% perdeuterated trifluoroethanol indicated that residues 8-17 were helic

89 CD spectra showed that trifluoroethanol induced significant alpha-helical struc

90 Trifluoroethanol induces alpha-helix in both human and m

91 ount of alpha-helix and that the addition of trifluoroethanol induces alpha-helix in both murine and

92 epared from readily available B2O3 and 2,2,2-trifluoroethanol, is as an effective reagent for the dir

93 ile in the more polar hexafluoropropanol and trifluoroethanol it is 562 and 571 nm, respectively.

94 ted by laser flash photolysis (LFP) in 2,2,2-trifluoroethanol (lambda = 580 nm, tau = 690 +/- 10 ns).

95 ride (2) in aqueous methanol, ethanol, 2,2,2-trifluoroethanol, n-propyl alcohol, isopropyl alcohol, a

96 -d (26 ps), 2-propanol-OD (40 ps), and 2,2,2-trifluoroethanol-O-d (14 ps) are longer than those recor

97 rs (methanol, 2-methyl-2-propanol, and 2,2,2-trifluoroethanol) on SmI2-initiated 5-exo-trig ketyl-ole

98 nd the dendrimeric SB056 in water and in 30% trifluoroethanol, on the other hand, yielded essentially

99 +) and unreactive substrate analogues, 2,2,2-trifluoroethanol or 2,3,4,5,6-pentafluorobenzyl alcohol,

100 The addition of trifluoroethanol or high ionic strength induced signific

101 n alpha-helical structure in the presence of trifluoroethanol or lipopolysaccharide.

102 me degree of beta-strand structures in water/trifluoroethanol or phosphate-buffered solutions.

103 ha-helical configurations in the presence of trifluoroethanol or phosphatidylcholine/phosphatidylseri

104 when the peptide is dissolved in n-octanol, trifluoroethanol or sodium dodecyl sulfate micelles, agg

105 ter than in highly ionizing solvents such as trifluoroethanol or trifluoroacetic acid.

106 r in the presence of trimethylamine N-oxide, trifluoroethanol, or a cationic surfactant.

107 ed by addition of dimethyl sulfoxide (DMSO), trifluoroethanol, or ethanol.

108 cated the degree of helicity in H2O, aqueous trifluoroethanol, or micelles.

109 ronsted acids of increasing strength, water, trifluoroethanol, phenol, and acetic acid, have been sys

110 The acidity of trifluoroethanol (pKa 12.4) resembles that of tRNA (12.9

111 he concentrations of the fluorinated alcohol trifluoroethanol promotes dissociation of both alpha 4H

112 (4)](-) (Ar = aryl; R = H, CH(3); L = water, trifluoroethanol) react smoothly with benzene at approxi

113 2-diol, 80% isopropanol, 80% ethanol and 40% trifluoroethanol showed that the organic solvent molecul

114 helical under all conditions if derived from trifluoroethanol solutions, and aggregated in a beta-she

115 benzene at approximately room temperature in trifluoroethanol solvent to yield methane and the corres

116 l induction of an alpha-helical structure by trifluoroethanol suffices to accelerate productive foldi

117 In the presence of 25% (v/v) trifluoroethanol (TFE) AcP undergoes partial unfolding a

118 te, and this is facilitated by solvent 2,2,2-trifluoroethanol (TFE) acting as a proton shuttle.

119 susceptible cells, as well as the ability of trifluoroethanol (TFE) and detergent systems to induce s

120 c strength solutions, and in the presence of trifluoroethanol (TFE) and dodecylphosphocholine (DPC) m

121 that promote aggregation in 25% (v/v) 2,2,2 trifluoroethanol (TFE) are different from those that pro

122 3,3,3-hexafluoroisopropanol (HFIP) and 2,2,2-trifluoroethanol (TFE) as reaction media is described.

123 ere we show that low concentrations of 2,2,2-trifluoroethanol (TFE) convert predominately unstructure

124 te of hen lysozyme formed in 60% (v/v) 2,2,2-trifluoroethanol (TFE) has been studied using hydrogen e

125 oteins in aqueous solutions containing 2,2,2-trifluoroethanol (TFE) have shown that the formation of

126 plete lack of secondary structure, while 40% trifluoroethanol (TFE) induces >90% helicity and is unpe

127 2,2,2-Trifluoroethanol (TFE) is known to stabilize peptide hel

128 2,2,2-Trifluoroethanol (TFE) is widely used to induce helix fo

129 ly), have been measured by using CD in water/trifluoroethanol (TFE) mixtures.

130 , and molecular dynamic simulations in water/trifluoroethanol (TFE) mixtures.

131 2,2,2-Trifluoroethanol (TFE) most stabilizes the alpha-helix-l

132 We describe a novel trifluoroethanol (TFE) or hexafluoropropan-2-ol (HFP) me

133 ith significant alpha-helical content in 30% trifluoroethanol (TFE) or in dodecylphosphocholine (DPC)

134 plished with Dowex 1 x 2 (CF3CH2O-) in 2,2,2-trifluoroethanol (TFE) or lithium trifluoroethoxide/TFE.

135 C spectra of IA(3) in water and in 23% 2,2,2-trifluoroethanol (TFE) shows that the individual residue

136 rmined by circular dichroism (CD) spectra in trifluoroethanol (TFE) solution are obtained.

137 termine its conformation in both aqueous and trifluoroethanol (TFE) solutions.

138 Trifluoroethanol (TFE) stabilized a native-like beta-tur

139 Trifluoroethanol (TFE) stabilized the secondary structur

140 ge in negative ion mode by trace addition of trifluoroethanol (TFE) to aqueous samples.

141 The binding of ethanol and 1,1,1-trifluoroethanol (TFE) to both the H(mv) and H(ox) forms

142 investigated in aqueous buffer and in 2,2,2-trifluoroethanol (TFE) using CD and NMR spectroscopy.

143 8 beta-strands, induced by dissolving in 50% trifluoroethanol (TFE) were monitored at neutral and low

144 In 30 vol-% trifluoroethanol (TFE), a single continuous helix is evi

145 elical conformation in the presence of 2,2,2-trifluoroethanol (TFE), a solvent known to stabilize hyd

146 olution was compared to the structure in 30% trifluoroethanol (TFE), and clear differences were obser

147 Circular dichroism (CD) in H2O, trifluoroethanol (TFE), and SDS micelles confirmed the i

148 In trifluoroethanol (TFE), Au(OAc(F))(CH2CH2OCH2CF3)(tpy) (

149 rine nucleosides in the fluorinated alcohols trifluoroethanol (TFE), hexafluoropropan-2-ol (HFP), and

150 se can be converted in vitro, by addition of trifluoroethanol (TFE), into amyloid fibrils of the type

151 elix-stabilizing cosolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kineti

152 and propanol and moderate concentrations of trifluoroethanol (TFE), or because of the appearance of

153 Upon addition of trifluoroethanol (TFE), significant shifts are observed

154 In the presence of 25% trifluoroethanol (TFE), the helicity of the peptide is 8

155 , including hexafluoroisopropanol (HFIP) and trifluoroethanol (TFE), to activate the electron-deficie

156 r solvolyses of 28 acid chlorides in 97% w/w trifluoroethanol (TFE)-water spanning over 10 (9) in rat

157 of small amounts of the helicogenic solvent trifluoroethanol (TFE).

158 peptide RGITVNGKTYGR, in water and in water/trifluoroethanol (TFE).

159 4-14; and helix 2, residues 20-29) in 2,2,2-trifluoroethanol (TFE).

160 r and in the hydrogen bond-promoting solvent trifluoroethanol (TFE).

161 the alpha-helix-rich conformation driven by trifluoroethanol (TFE).

162 (alphaB) is disordered but inducible in 40% trifluoroethanol (TFE).

163 iposomes and are soluble in SDS micelles and trifluoroethanol (TFE).

164 or alpha-helix formation in water and in 50% trifluoroethanol (TFE).

165 k(d) from tert-butylbenzene (tBuPh) to 2,2,2-trifluoroethanol (TFE).

166 mediate (10-20% v/v) concentrations of 2,2,2-trifluoroethanol (TFE).

167 rimethylamine N-oxide (TMAO) and the solvent trifluoroethanol (TFE).

168 ions, including aqueous solutions containing trifluoroethanol (TFE).

169 ly alpha-helical secondary structures in 99% trifluoroethanol (TFE)/H(2)O.

170 re recorded in the solvent system (40% 2,2,2-Trifluoroethanol (TFE)/water), which gave the largest st

171 in the presence of the lipid mimetic solvent trifluoroethanol (TFE; 50% v/v).

172 orted for solvolyses in acetone/water, 2,2,2-trifluoroethanol(TFE)/water, and TFE/ethanol mixtures.

173 (i.e., MeOH and EtOH) and fluorinated (i.e., trifluoroethanol, TFE) alcohols on the secondary structu

174 pectively, 227 and 14 times more slowly with trifluoroethanol than the parent benzhydrylium ion (Ph)2

175 udies indicated that, in the presence of 50% trifluoroethanol, the HIV-1 peptide adopts an alpha-heli

176 f methanol, sodium dodecyl sulfate (SDS), or trifluoroethanol, the sulfoxide yield increases 3-5 time

177 site-selective substitutive oxygenation with trifluoroethanol to afford the desired trifluoromethylar

178 structure in aqueous solution and convert in trifluoroethanol to alpha-helix (PEVT, CEEEI, DispRep) a

179 ous solvents ranging from hydrophilic (e.g., trifluoroethanol) to hydrophobic (e.g., n-propanol).

180 ere assessed using a detailed CD analysis in trifluoroethanol, trifluoroethanol-water mixtures, sodiu

181 otein's guanidine hydrochloride-unfolded and trifluoroethanol-unfolded states.

182 In 50% trifluoroethanol (v/v), the peptides are 45% helical as

183 a detailed CD analysis in trifluoroethanol, trifluoroethanol-water mixtures, sodium dodecyl sulfate

184 40-80%) helical in trifluoroethanol and most trifluoroethanol-water mixtures.

185 ear magnetic resonance spectra were taken in trifluoroethanol-water mixtures.

186 s, and structural modeling suggested that in trifluoroethanol/water (1:1) helical subdomains existed

187 ing rate ratios in 40% ethanol/water and 97% trifluoroethanol/water (solvents of similar ionizing pow

188 Both the Ad12 and Ad5 peptides dissolved in trifluoroethanol/water mixtures were found to adopt regu

189 In trifluoroethanol/water mixtures, NAC and NAC-(19-35)-pep

190 is essentially disordered in DMSO, water or trifluoroethanol/water.

191 y observed when weaker nucleophiles, such as trifluoroethanol, were employed.

192 reased markedly in the presence of 50% 2,2,2-trifluoroethanol, which causes loss of tertiary structur

193 cyano stretch of 1 to a higher wavenumber in trifluoroethanol, which is consistent with 1 participati

194 phaeroides PufX exhibited 47% alpha-helix in trifluoroethanol while the core segment containing 43 am