ライフサイエンスコーパス: trophoblast

1 found entrapped in the cytoplasm of the GDM-trophoblast.

2 ss of cell adhesion in methylation-deficient trophoblast.

3 rentiated along multiple lineages, including trophoblast.

4 ognize the fetal HLA-C molecules on invading trophoblast.

5 pression in the ICM and its silencing in the trophoblast.

6 that recognize allogeneic HLA-C molecules on trophoblast.

7 xpression of HLA-G, a marker of extravillous trophoblast.

8 mediating the down-regulation of SERT in GDM trophoblast.

9 DLX5 is expressed in human but not in murine trophoblast.

10 n of imprinting gene expression in the molar trophoblast.

11 y which CDX2 maintains repression of OCT4 in trophoblast.

12 to HCMV-infected primary fetal extravillous trophoblasts.

13 n primary human placental organ cultures and trophoblasts.

14 ory signature and a disturbed crosstalk with trophoblasts.

15 to bile acid decreased PRDX3 level in human trophoblasts.

16 ches the terminal fate of these hESC-derived trophoblasts.

17 trol or Dex-treated cultured third-trimester trophoblasts.

18 and decreased proliferation of labyrinthine trophoblasts.

19 ar results were obtained with HUVEC and HTR8 trophoblasts.

20 ly target InsRs in fetally derived placental trophoblasts.

21 nderstanding how dNK responses to allogeneic trophoblast affect the outcome of pregnancy.

22 Viral antigen localizes in trophoblast and endothelial cells in the placenta, and e

23 a paracrine and autocrine manner to protect trophoblast and non-trophoblast cells from ZIKV infectio

24 ese include proteins known to be involved in trophoblast and placenta physiology.

25 lethal phenotypes in mice, cause defects in trophoblast and placental development, and/or affect con

26 ERT is encoded by the same gene expressed in trophoblast and platelets.

27 ional silencing of the CYP27A1 gene in human trophoblast and rat adrenocortical cells reduced the exp

28 eam elements were significantly lower in GDM-trophoblast and showed no response to the insulin stimul

29 Similarly, the 5-HT uptake rates of GDM-trophoblast and the SERT expression on their surface wer

30 Proliferation and apoptosis of trophoblasts and fusion of the mononucleated state to th

31 It plays a role in differentiation of trophoblasts and helps in normal placentation and format

32 fection induced autophagic activity in human trophoblasts and pharmacological inhibition limited ZIKV

33 sence of both FGF2 and BMP4 by conversion to trophoblast, and especially syncytiotrophoblast, whereas

34 y tube formation involving HUVEC and/or HTR8 trophoblasts, and aortic ring endothelial cell outgrowth

35 and BeWo cells and in primary human villous trophoblasts, and this induction was abrogated by CH2231

36 ry effect of MSU crystals was accompanied by trophoblast apoptosis and decreased syncytialization.

37 hways that govern the terminal fate of these trophoblasts are not understood.

38 gnancy disorders characterized by failure of trophoblast arterial transformation.

39 During pregnancy, placental trophoblasts at the feto-maternal interface produce a br

40 o secretions from the placenta or from model trophoblast barriers that had been exposed to altered ox

41 trophoblast cells, including basal chorionic trophoblast (BCT) cells located at the chorioallantoic i

42 xtracellular vesicles (EVs), which influence trophoblast behaviour during the implantation process.

43 , which has been instrumental in elucidating trophoblast biology.

44 rved Grhl2-coordinated gene network controls trophoblast branching morphogenesis, thereby facilitatin

45 nists and can induce BCRP in human placental trophoblasts by activating AhR.

46 Trophoblasts can terminally differentiate to either extr

47 owever, the regulatory mechanisms that guide trophoblast cell fate decisions during placenta developm

48 olecular mechanism by which folate regulates trophoblast cell function.

49 ression of a broad set of genes required for trophoblast cell fusion and hormonogenesis.

50 human trophoblast cells and an extravillous trophoblast cell line (HTR8), from first and second trim

51 Moreover, silencing of PRDX3 in trophoblast cell line HTR8/SVneo induced growth arrest a

52 n, and decreased cellular proliferation in a trophoblast cell line.

53 nate the specific regulation of PGF in human trophoblast cell lines.

54 regnant women serum, and is known to promote trophoblast cell proliferation and adhesion.

55 This protection was due to a placental trophoblast cell-autonomous effect of autophagic activit

56 ntinue to provide a model for studying early trophoblast cells (TB), but many questions have been rai

57 PRDX3 protected trophoblast cells against mitochondrial dysfunction and

58 In this study, we show that primary human trophoblast cells and an extravillous trophoblast cell l

59 the cell-cell interactome of fetal placental trophoblast cells and maternal endometrial stromal cells

60 d production of progesterone 2-fold in human trophoblast cells and of corticosterone by 90% in rat ad

61 ICP placentas as well as bile acids-treated trophoblast cells and villous explant in vitro.

62 we demonstrate that HCV-RNA sensing by human trophoblast cells elicits a strong antiviral response th

63 Invading placental trophoblast cells express human leukocyte antigen class

64 Isolation and in vitro culture of Sca-1(+) trophoblast cells from both differentiated TS cell cultu

65 ocrine manner to protect trophoblast and non-trophoblast cells from ZIKV infection.

66 isplayed co-regulation and were expressed in trophoblast cells in a similar domain as in mouse placen

67 ith placebo and n-3 and in vitro in isolated trophoblast cells in response to DHA and EPA treatment.

68 chanisms by which LDA influences PE-affected trophoblast cells in vitro are by modulating cytokine se

69 We found that HCV-RNA sensing by human trophoblast cells induces robust upregulation of type I/

70 During human pregnancy, fetal trophoblast cells invade the decidua and remodel materna

71 We propose that mTOR folate sensing in trophoblast cells matches placental nutrient transport,

72 Inflammasome activation in trophoblast cells of women with preeclampsia corroborate

73 Invading trophoblast cells promote blood flow to the conceptus by

74 from pregnant women or conditioned medium of trophoblast cells promoted endometrium receptivity in vi

75 How trophoblast cells regulate their differentiation into a

76 vitro inhibition of 20alpha-HSD activity in trophoblast cells reversed PI-based cART-induced decreas

77 ver, Elf5 is also present in differentiating trophoblast cells that have ceased to express other TSC

78 uterus and interact with invading placental trophoblast cells that transform the maternal arteries t

79 platelets and inflammasome activation within trophoblast cells through purinergic signaling.

80 We used cultured primary human trophoblast cells to test the hypothesis that mechanisti

81 Inflammasome activation in trophoblast cells triggers a PE-like phenotype, characte

82 However, the role of PRDX3 in placental trophoblast cells under ICP is not fully understood.

83 ing implantation defects, disorganization of trophoblast cells, fewer uterine natural killer (uNK) ce

84 ion factor, is highly expressed in chorionic trophoblast cells, including basal chorionic trophoblast

85 human fetal placenta itself, mainly through trophoblast cells, is able to induce homeostatic M2 macr

86 ts and purinergic inflammasome activation in trophoblast cells, uncovering a novel thromboinflammator

87 helial component of the placenta consists of trophoblast cells, which possess the capacity for multil

88 nal-fetal interface through interaction with trophoblast cells.

89 lization have their counterpart expressed in trophoblast cells.

90 tive stress and mitochondrial dysfunction in trophoblast cells.

91 transition from progenitor to differentiated trophoblast cells.

92 cted to trophoblast giant cells and invasive trophoblast cells.

93 cterized the FOSL1 regulatory pathway in rat trophoblast cells.

94 ed both endocrine and invasive properties of trophoblast cells.

95 , to a lesser extent, JUN in differentiating trophoblast cells.

96 FOSL) 1, and FOSL2) proteins in extravillous trophoblast cells.

97 thylated ESCs that are prone to acquire some trophoblast characteristics, Plet1 is required to confer

98 chemotactic protein-1, when cocultured with trophoblasts compared with control macrophages.

99 tablish important patterning cues within the trophoblast compartment by promoting differentiation tow

100 anscription factor with pivotal roles in the trophoblast compartment, where it reinforces a trophobla

101 n patterns that were, in part, indicative of trophoblast competence.

102 ression restricted to placental extravillous trophoblasts contributes to maternal tolerance to the se

103 rophoblast differentiation by modulating the trophoblast cytoskeleton.

104 tation and is tightly regulated and involves trophoblast-decidual cell interaction.

105 based on high throughput analysis of primary trophoblasts, derived from term human placenta and cultu

106 cts of heme on the viability and fusion of a trophoblast-derived cell line (BeWo).

107 n specific domains of DLX3 and GCM1 in human trophoblast-derived cells by performing immunoprecipitat

108 al compartment, it must evade restriction by trophoblast-derived IFNlambda1 and other trophoblast-spe

109 Trophoblast-derived pregnancy-associated plasma protein

110 However, mechanisms controlling human trophoblast development are largely unknown.

111 methylation plays a major role in regulating trophoblast development but that imprinting of the key p

112 We dissected the role of DNA methylation in trophoblast development by performing mRNA and DNA methy

113 ulators in luminal epithelium proliferation, trophoblast development, and uNK maturation during pregn

114 ed, but less is known about its roles during trophoblast development, the extraembryonic lineage that

115 some remodeling at the Nanog enhancer during trophoblast development.

116 lf-renewing alternative state permissive for trophoblast development.

117 rophoblast progenitor cell fate during human trophoblast development.

118 ses intrauterine lethality due to defects in trophoblast development.

119 from placental villi at term and colonies of trophoblast differentiated from embryonic stem cells (ES

120 Defects in remodelling and trophoblast differentiation are associated with severe g

121 nase 1 (LIMK1) expression regulates invasive trophoblast differentiation by modulating the trophoblas

122 -treated cells, and preventing the premature trophoblast differentiation commonly observed in PE.

123 Feto-placental macrophages regulate villous trophoblast differentiation during placental development

124 HIF is known to play a role in trophoblast differentiation in rodents; however, its rol

125 ation in rodents; however, its role in human trophoblast differentiation is poorly understood.

126 methylation-regulated genes associated with trophoblast differentiation that are involved in cell ad

127 trophoblast lineage identity and modulating trophoblast differentiation.

128 , as Elf5 overexpression triggers precocious trophoblast differentiation.

129 as robustly induced following stimulation of trophoblast differentiation.

130 of the human-specific regulatory network of trophoblast differentiation.

131 us, miR-515-5p may serve a key role in human trophoblast differentiation; its aberrant up-regulation

132 ation, amniotic and yolk sac cavitation, and trophoblast diversification.

133 Trophoblast elongation occurred on day 14 in wild-type (

134 sia, is the failure of invading extravillous trophoblast (EVT) cells to remodel the maternal uterine

135 n leukocyte antigen-G+ (HLA-G+) extravillous trophoblasts (EVT) are rare cells that are believed to p

136 natural killer cells (dNK) and extravillous trophoblasts (EVT) at the maternal-fetal interface was s

137 pregnancy, semiallogeneic fetal extravillous trophoblasts (EVT) invade the uterine mucosa without bei

138 in floating chorionic villi or extravillous trophoblasts (EVTs) at the anchoring villi.

139 modeling before colonization by extravillous trophoblasts (EVTs); however, the trigger for their infi

140 d maternal vascular remodeling (extravillous trophoblasts, EVTs).

141 , we demonstrate that cultured primary human trophoblasts express ACKR2 far more strongly than genes

142 being permissive for ZIKV infection, primary trophoblasts expressed multiple putative ZIKV cell entry

143 es to orchestrate stem versus differentiated trophoblast fate.

144 age and regulates gene expression to promote trophoblast fate.

145 study, we show that primary human placental trophoblasts from non-exposed donors (n = 20) can be inf

146 beta3 (from platelets) and alphaVbeta3 (from trophoblasts) from HPA-1a(+) donors was demonstrated by

147 Perturbed trophoblast function and impaired placental development

148 e hypothesized that LDA influences placental trophoblast function and reverses PE-associated abnormal

149 in regulation of innate immune responses and trophoblast function during pregnancy.

150 mechanisms of action of LDA, particularly on trophoblast function, are unclear.

151 is impaired placentation resulting from poor trophoblast function, which reduces blood flow to the fe

152 In the presence of forskolin, which triggers trophoblast fusion, heme inhibits BeWo cell fusion throu

153 imp1-dependent regulatory networks governing trophoblast gene expression.

154 t1 levels favour differentiation towards the trophoblast giant cell lineage, whereas lack of Plet1 pr

155 selective regions of the genome in parietal trophoblast giant cells (p-TGCs) of the mouse placenta.

156 essential for specification of spiral artery trophoblast giant cells (SpA-TGCs) that invade and remod

157 ulted in differentiation of blastomeres into trophoblast giant cells (TGCs), suggesting that geminin

158 FOSL1 expression is restricted to trophoblast giant cells and invasive trophoblast cells.

159 ed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac.

160 ut expressed on the cell surface of TSCs and trophoblast giant cells.

161 d a reduced DNA content in endoreduplicating trophoblast giant cells.

162 r before a protective zone of mature villous trophoblast has been established.

163 Also, the ability of hESCs to form bona fide trophoblasts has been intensely debated.

164 sion of miR-515-5p in cultured primary human trophoblasts impaired SynT differentiation and specifica

165 nsporter, SERT to the plasma membrane of the trophoblast in placenta.

166 We have examined hESC-derived trophoblasts in the light of stringent criteria that wer

167 smic reticulum stress-response activation in trophoblasts in vitro.

168 ofiles of term intravillous and extravillous trophoblasts, including the transcriptome of the multinu

169 These data suggest that trophoblast infection may be a mechanism of transplacent

170 l macrophages, leading to altered macrophage-trophoblast interaction, is involved in preeclampsia.

171 such critical regulator, programming primary trophoblasts into progenitors of the invasive differenti

172 fetus, progenitors develop into extravillous trophoblasts invading the maternal uterus and its spiral

173 Dysregulation of human trophoblast invasion and differentiation can result in p

174 with placental architecture displaying poor trophoblast invasion and spiral artery development in th

175 mentary approaches, including HUVEC-mediated trophoblast invasion in nude mice, in vitro three-dimens

176 been demonstrated to facilitate extravillous trophoblast invasion into maternal decidua during the fi

177 ng to HLA-C is a generic mechanism promoting trophoblast invasion into the decidua.

178 Human trophoblast invasion of decidualized endometrium is esse

179 blishment of the correct cellular milieu and trophoblast invasion, all of which involve the action of

180 Chemokines CXCL12 and CXCL16, important for trophoblast invasion, were analysed further and expressi

181 dhesion and migration, potentially affecting trophoblast invasion.

182 other chemokines, known to promote placental trophoblast invasion.

183 Trophoblast is the primary epithelial cell type in the p

184 human placenta and its different epithelial trophoblasts is crucial for a successful pregnancy.

185 owth factor (PlGF), abundantly produced from trophoblasts is involved in placental angiogenesis.

186 We showed previously that trophoblasts isolated from full-term placentas resist in

187 xpression in JEG3 cells and in primary human trophoblasts isolated from placenta.

188 BP51 and decidual cell (DC) and interstitial trophoblast (IT) markers, vimentin and cytokeratin, resp

189 on between the inner cell mass (ICM) and the trophoblast layer of the blastocyst is known to occur, b

190 to the host mouse and include small areas of trophoblast-like cells.

191 selectively expressed in the extraembryonic trophoblast lineage and regulates gene expression to pro

192 1 (Plet1), for its function in establishing trophoblast lineage identity and modulating trophoblast

193 velopment of progenitors of the extravillous trophoblast lineage in the human placenta.

194 y detected in precursors of the extravillous trophoblast lineage, forming cell columns anchored to th

195 n the placenta, expression is limited to the trophoblast lineage, where it remains highly expressed u

196 BRG1-HDAC1 interactions were enriched in the trophoblast lineage.

197 BRG1 is necessary for Nanog silencing in the trophoblast lineage.

198 specific functional activities unique to the trophoblast lineage.

199 generation of the inner cell mass (ICM) and trophoblast lineages comprises upregulation of Nanog exp

200 nodal signaling to obtain cells that express trophoblast markers.

201 alling kinases, JNK and FAK, and stimulating trophoblast migration.

202 blished an in vitro invasion-differentiation trophoblast model.

203 Manipulation of Notch1 in primary trophoblast models demonstrated that the receptor promot

204 f the defect on insulin receptor (IR) in the trophoblast of the gestational diabetes mellitus (GDM)-a

205 We identified ZIKV within trophoblasts of the maternal and fetal placenta, consist

206 minally differentiate to either extravillous trophoblasts or syncytiotrophoblasts.

207 mmunostaining was undetected in interstitial trophoblasts (P < 0.05).

208 ifically regulate the endocrine and invasive trophoblast phenotypes.

209 unctions as a folate sensor in primary human trophoblast (PHT) cells.

210 unctions as a folate sensor in primary human trophoblast (PHT) cells.

211 investigate ZIKV infection of primary human trophoblasts (PHTs), which are the barrier cells of the

212 al mTORC1 and mTORC2 signaling and decreased trophoblast plasma membrane System A and L amino acid tr

213 t decreased PRDX3 by excessive bile acids in trophoblasts plays a critical role in the pathogenesis a

214 By contrast, the ESC-derived trophoblasts possess a wide range of attachment factors

215 increase in 1,25(OH)2D3] and the evidence of trophoblast production and secretion of vitamin D metabo

216 mportant function of OVOL1 as a regulator of trophoblast progenitor cell fate during human trophoblas

217 -290 cluster gene (Mirc5) results in reduced trophoblast progenitor cell proliferation and a reduced

218 arlier stages involving development of human trophoblast progenitor cells (TBPCs), which give rise to

219 Here we report that HCMV replicates in trophoblast progenitor cells (TBPCs)-precursors of the m

220 MATERIALS AND Human trophoblast progenitor cells were isolated at 7-14 wk of

221 ic redundancy of Gata3 with paralog Gata2 in trophoblast progenitors ensures the successful progressi

222 onic villi and amniotic epithelial cells and trophoblast progenitors in amniochorionic membranes-that

223 In trophoblast progenitors, GATA factors directly regulate

224 K4me3, H3K27me3, and CpG methylation maps of trophoblast progenitors, purified using the surface mark

225 s proliferation and survival of extravillous trophoblast progenitors.

226 ide reverse transcriptase inhibitors reduced trophoblast progesterone production in vitro.

227 PI effects on trophoblast progesterone production were assessed in vit

228 Overexpression of TPalpha enhanced trophoblast proliferation and syncytialisation.

229 fetal growth by inhibiting WNT signaling and trophoblast proliferation.

230 bed imprinting, the upregulated DLX5 affects trophoblast proliferation.

231 he HLA-C allotype HLA-C2, expressed by fetal trophoblasts, reduces the risk of developing pregnancy c

232 2, temporally increases during the period of trophoblast remodeling during elongation.

233 eginning three weeks after conception, using trophoblast retrieval and isolation from the cervix (TRI

234 Stage-specific gene deletion in trophoblasts reveals that loss of both GATA genes, but n

235 tiation and thus may be pivotal in balancing trophoblast self-renewal and differentiation.

236 Notably, in vitro experiments with trophoblasts showed increased production and secretion o

237 vels than minor allele homozygotes; decidual trophoblasts showed strong CXCR3 immunoreactivity.

238 by trophoblast-derived IFNlambda1 and other trophoblast-specific antiviral factors and/or use altern

239 Specifically, trophoblast-specific DNA methylation is linked to the si

240 provides genetic evidence that impairment of trophoblast-specific GATA2/GATA3 function could lead to

241 However, trophoblast-specific GATA3 function is dispensable for e

242 aracteristics, Plet1 is required to confer a trophoblast-specific gene expression pattern, including

243 d TFAP2A indicated that they directly couple trophoblast-specific gene induction with suppression of

244 a transgenic animal by exploiting placental trophoblast-specific gene manipulation using lentiviral

245 ring were evaluated for effects of placental trophoblast-specific InsR deficiency on stress sensitivi

246 Male, but not female, mice with placental trophoblast-specific InsR deficiency showed a significan

247 Complementary in vivo experiments using trophoblast-specific lentiviral delivery of FOSL1 short

248 Knockdown of placental sFlt1 with a trophoblast-specific transgene caused placental vascular

249 ls (TGCs), suggesting that geminin regulates trophoblast specification and differentiation.

250 Trophoblast stem (TS) cells in the mouse derive from the

251 Here we show that in trophoblast stem (TS) cells, Esrrb is a downstream targe

252 CDX2 directly, we performed CDX2 ChIP-Seq on trophoblast stem (TS) cells.

253 Through embryonic stem (ES) to trophoblast stem (TS)-like cell reprogramming by introdu

254 ely hampered by the lack of a representative trophoblast stem cell (TSC) model with the capacity for

255 ophoblast compartment, where it reinforces a trophoblast stem cell (TSC)-specific transcriptional cir

256 ogeny, from which we derived the first human trophoblast stem cell line.

257 Bona fide trophoblast stem cell lines and extra-embryonic endoderm

258 Trophoblast stem cells (TSCs) are derived from the early

259 ropriate self-renewal and differentiation of trophoblast stem cells (TSCs) are key factors for proper

260 Trophoblast stem cells (TSCs) arise from the first cell

261 Trophoblast stem cells (TSCs) give rise to specialized c

262 bryonic stem cells (ESCs) and extraembryonic trophoblast stem cells (TSCs) in a three-dimensional sca

263 Trophoblast stem cells (TSCs) retain the capacity to sel

264 elete the core PRC2 protein EED in F1 hybrid trophoblast stem cells (TSCs), which undergo imprinted i

265 mutant placenta and in vitro differentiated trophoblast stem cells allow us to identify Blimp1-depen

266 ited decreased decay rates in differentiated trophoblast stem cells derived from KO blastocysts.

267 cience that 3D co-cultures of mouse ESCs and trophoblast stem cells self-organize into embryo-like st

268 We recently demonstrated in mouse trophoblast stem cells that hypoxia-inducible factor-2 (

269 cells, facilitated transdifferentiation into trophoblast stem cells, and impaired differentiation of

270 region of the postimplantation embryo and in trophoblast stem cells.

271 blasts induces stable, transgene-independent trophoblast stem-like cells (iTSCs).

272 Trophoblasts stimulated by these factors express more CY

273 and establish a new mechanism through which trophoblast sublineages are specified.

274 Different trophoblast subtypes are responsible for gas/nutrient ex

275 arkers of both junctional zone and labyrinth trophoblast subtypes in a manner comparable to establish

276 bour the potential to differentiate into all trophoblast subtypes of the placenta.

277 he lack of FKBP51 expression in interstitial trophoblasts suggests unopposed constitutive GR action.

278 Protein expression of the trophoblast survival factor insulin-like growth factor-2

279 We report that trophoblasts that possess these properties can indeed be

280 d layers of protection and relies heavily on trophoblasts, the fetal-derived cells that comprise the

281 pitulate the antiviral properties of primary trophoblasts through the constitutive release of type II

282 s to study susceptibility of human placental trophoblast to ZIKV: cytotrophoblast and syncytiotrophob

283 ong-range chromatin looping mediated by core trophoblast transcription factors as the mechanism contr

284 act with integrins along the surfaces of the trophoblasts, triggering the activation of two signallin

285 c (ESCs) but hypomethylated and expressed in trophoblast (TSCs) stem cells are very rare and may have

286 dditionally expressed in clusters of villous trophoblasts underlying the syncytium, suggesting that t

287 ation-related and physiological processes in trophoblast via both imprinting-dependent and -independe

288 ighly purified HLA-G+ EVT and HLA-G- villous trophoblasts (VT) were isolated.

289 expression in the three germ layers and the trophoblast was abnormal in the EBs of tetraploid ESCs c

290 orted populations of primary first-trimester trophoblasts, we evaluated the first stage of EVT differ

291 yndecan-2, as well as macrophage adhesion to trophoblasts were diminished.

292 In addition, cultured human trophoblasts were incubated with (13)C-cholecalciferol t

293 n = 6 for each group), and the platelets and trophoblasts were isolated to determine the IR activity,

294 signaling leads to formation of extravillous trophoblast, whereas loss of activin/nodal inhibition le

295 ly, MR766 is highly trophic toward primitive trophoblast, which may put the early conceptus of an inf

296 s are more permissive to ZIKV infection than trophoblasts, which may be refractory to infection.

297 nd that it was also expressed in a subset of trophoblast within the chorion and labyrinth layer of th

298 mechanism by which ES cells communicate with trophoblasts within the blastocyst to increase their abi

299 ntly decreased FA esterification in isolated trophoblasts without affecting FA oxidation.

300 -FLR strain can replicate in human placental trophoblasts without host cell destruction, thereby serv