ライフサイエンスコーパス: tryptic digest

1 entative of the range in peptides found in a tryptic digest.

2 with both peptide standards and a HeLa cell tryptic digest.

3 eptides detected from a Shewanellaoneidensis tryptic digest.

4 proximately 700 peptides for a S. oneidensis tryptic digest.

5 ndem mass spectrometry (MS/MS) analysis of a tryptic digest.

6 ified as serine 461 by HPLC-MS analysis of a tryptic digest.

7 s of angiotensin peptides and a cytochrome c tryptic digest.

8 liquid chromatography-mass spectrometry of a tryptic digest.

9 mino acid sequence by Edman degradation of a tryptic digest.

10 ted form, prepared in a bovine serum albumin tryptic digest.

11 opeptides can be isolated selectively from a tryptic digest.

12 cities of 130-420 for analytes from proteome tryptic digests.

13 ealed by the appearance of a new fragment in tryptic digests.

14 nalysis of both soluble and membrane protein tryptic digests.

15 by SDS-PAGE and LC-MS/MS analysis of in-gel tryptic digests.

16 pplied for the study of whole-proteome mouse tryptic digests.

17 for CE/MS of peptides, proteins, and protein tryptic digests.

18 Dissociation (HCD) spectra or spectra of non-tryptic digests.

19 s conducted with peptide solutions mimicking tryptic digests.

20 verified using standard peptides and protein tryptic digests.

21 romatography/tandem mass spectrometry of NFT tryptic digests.

22 tandem mass spectrometry (MS/MS) analysis of tryptic digests.

23 dem mass spectrometry (LC-MS/MS) analyses of tryptic digests.

24 luding neuronal tissue releasate and protein tryptic digests.

25 ve to abundant nonphosphorylated peptides in tryptic digests.

26 a 1% false-discovery rate (FDR) from a yeast tryptic digest (95% confidence, p = 0.019).

27 imeric IGPS from Escherichia coli (HisHF) by tryptic digest and FABMS.

28 or measurements of a complex fungal proteome tryptic digest and provide improved confidence or number

29 ilico identify the peptides from the E. coli tryptic digest and show the increased confidence in pept

30 c acid improved the signal-to-noise ratio of tryptic digests and gave a 3-fold increase in the number

31 ted by partial amino acid microsequencing of tryptic digests and immunologic reactivity.

32 cations were studied by LC-MS/MS analyses of tryptic digests and included DTT-reversible events, e.g.

33 approach to identification of peptides (from tryptic digests) and to separation of charge-state distr

34 was evaluated using a Shewanella oneidensis tryptic digest, and approximately 15-amol detection limi

35 ior to cleanup by immunoaffinity extraction, tryptic digest, and preconcentration by solid-phase extr

36 The photolabeled kinase was subjected to tryptic digest, and the fragments were separated by chro

37 f fluorescein-labeled model peptides and BSA tryptic digest are demonstrated using the microchip HPLC

38 n, some phosphorylated peptides generated by tryptic digest are small and hydrophilic and, thus, are

39 pmol or less of isoaspartate and works with tryptic digests as well as intact proteins.

40 On-chip tryptic digest assays were then performed on the capture

41 pray ionization mass spectrometry mapping of tryptic digests before and after liver transplantation.

42 mics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass

43 ade in terms of the capability of separating tryptic digests between the mixed-mode phase and C18 rev

44 quantities (100 ng) of a Pyrococcus furiosus tryptic digest, but with mass-limited amounts (5 ng) CE

45 Analysis of tryptic digests by MALDI-TOF MS does not provide enough

46 Employing a Saccharomyces cerevisiae tryptic digest, careful consideration of several perform

47 is by MALDI-FT-ICR-MS, de novo sequencing of tryptic digested CHH by nano-LC/ESI-Q-TOF MS and intact

48 ntiated from nonmodified peptides in complex tryptic digests created upon proteolysis of proteins aft

49 racterization of products from these partial tryptic digests demonstrated that approximately 90% of t

50 ional mapping of phosphopeptides in complete tryptic digests demonstrated that the reduced phosphoryl

51 Mass spectrometry of tryptic digests determined the sole linkage point to be

52 online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achi

53 produce missed cleavage peptides in protein tryptic digests even at prolonged digestion times.

54 lack widow spiders and performed in-solution tryptic digests followed by MS/MS analysis to identify n

55 were determined by mass spectral analyses of tryptic digest fragments of cross-linked hemoglobin, emp

56 Peptide sequence analysis of a tryptic digest from immunoaffinity-purified Pa showed 10

57 tified by mass spectrometric sequencing of a tryptic digest from the protein band on SDS-PAGE associa

58 The LC-MS chromatographic peak profiles of tryptic digests from OXY, bovine Hb, human Hb, and equin

59 erine-containing peptide was identified from tryptic digests from Sulfolobus solfataricus P1 by liqui

60 For yeast cytosolic tryptic digests > 100,000 polypeptides were detected, an

61 e separation of nucleic acids, proteins, and tryptic digests in combination with UV absorbance detect

62 Peptide sequences identified in the 70 kDa tryptic digest include iPLA(2)beta residues 7-53, sugges

63 ixtures of BSA and human serum albumin (HSA) tryptic digests indicated that ion series searches with

64 Typically, TTR or its tryptic digest is analyzed by MALDI-TOF MS, liquid chrom

65 targeting cysteine-containing peptides in a tryptic digest is described.

66 ing a 10-1000 fold-excess of enzyme, (v) the tryptic digest is directly transferred to a perfusion di

67 ngly and multiply phosphorylated peptides in tryptic digests is demonstrated at low-nanomolar protein

68 resis-tandem mass spectrometry (CE-MS/MS) of tryptic digests is described.

69 Using peptide mapping of tryptic digests, LC/MS, and amino acid sequence analysis

70 it-lamp stereophotomicroscopy, western blot, tryptic-digest/mass spectrometry electrospray ionisation

71 s spectrometry (MS/MS) analysis of an in-gel tryptic digest matched the protein sequence of thimet ol

72 kephalin and angiotensin II, spiked in a BSA tryptic digest matrix at different concentrations.

73 e containing 20 fmol of human growth hormone tryptic digest mixed with the bovine serum protein diges

74 ximately 400 for the separation of a complex tryptic digest mixture when the sample preparation inclu

75 With only 100 mug of tryptic digested, nonstimulated HeLa protein and 45 h of

76 okinetic elution and sample stacking using a tryptic digest of 16 proteins to maximize peptide identi

77 ed peptides were purified from an exhaustive tryptic digest of [32P]ANPP-labeled Na+/K(+)-ATPase.

78 ilar results were obtained when evaluating a tryptic digest of a cellular lysate, representing a more

79 ure phosphoserine-containing peptides from a tryptic digest of a complex peptide mixture in which its

80 Analysis of a tryptic digest of a covalently modified IGF-1R kinase fr

81 strated by fractionating the separation of a tryptic digest of a known protein mixture onto the micro

82 r isoelectric focusing (IEF) of a methylated tryptic digest of a mixture of alpha-S-casein and beta-c

83 ability to separate peptide mixtures, e.g., tryptic digest of a protein, is illustrated and compared

84 electrospray interface for the analysis of a tryptic digest of a sample of intermediate protein compl

85 of identified peptides from an analysis of a tryptic digest of a yeast whole cell lysate.

86 Mass spectrometry analysis of a tryptic digest of acetylated AcsA (AcsA(Ac)) identified

87 strated for a mock mixture of peptides and a tryptic digest of alphaS1-casein.

88 differentially labeled proteomic sample of a tryptic digest of an E. coli lysate.

89 In addition, the analysis of a tryptic digest of apomyoglobin by nanoLC-dual ESI-FT-ICR

90 For the Glu-C/tryptic digest of apomyoglobin, approximately 66% of the

91 The intact and the tryptic digest of AuNCs@ew were characterized by mass sp

92 a carboxyl-terminal fragments from a partial tryptic digest of beta while DAP-Q labels only the carbo

93 In the case of analyzing a tryptic digest of beta-casein, conventional MALDI MS rev

94 as performed in the presence of a background tryptic digest of bovine albumin.

95 Application of the method to a tryptic digest of bovine coagulation factor V resulted i

96 yses of complex biological samples such as a tryptic digest of bovine serum albumin and a carnitine s

97 peptides from injection of only 1 pmol of a tryptic digest of bovine serum albumin using an eluent f

98 illary LC for the analysis of substance P, a tryptic digest of bovine serum albumin, and a phosphopep

99 was initially evaluated in the analysis of a tryptic digest of bovine serum albumin.

100 t was added as a trace marker component to a tryptic digest of bovine serum proteins or to a human gr

101 LC-MS/MS analysis of a tryptic digest of BSA demonstrated that these cleavages

102 A Chromeo P503 labeled tryptic digest of BSA was used as a complex mixture to a

103 Peptide sequences of four peptides from a tryptic digest of cytochrome c (approximately 1 pmol dep

104 The approach is illustrated by examining a tryptic digest of cytochrome c and by identifying a pept

105 (reference standard), the accurate mass of a tryptic digest of cytochrome c was measured.

106 bilities of 2D FT-ICR MS are explored with a tryptic digest of cytochrome c with both ECD and IRMPD a

107 For a tryptic digest of cytochrome c, approximately 78% of the

108 ion trap mass spectrometry and compared to a tryptic digest of Deinococcus radiodurans.

109 ber of peptides and proteins identified in a tryptic digest of E. coli cell lysate increased by 13% a

110 the enzyme transglutaminase 2) or the peptic-tryptic digest of gliadin (in native and deamidated form

111 e peptide-specific hybridomas responded to a tryptic digest of HEL or to naturally processed HEL pept

112 ously uncharacterized phosphopeptides from a tryptic digest of human beta 4 integrin, isolated from c

113 igh performance liquid chromatography of the tryptic digest of inactivated enzyme yields a single rad

114 o distinguish the cross-linked peptides in a tryptic digest of IRCX-cross-linked ubiquitin.

115 wing fast protein liquid chromatography of a tryptic digest of MAP2, suggesting that multiple modific

116 o acid sequence coverage was attained from a tryptic digest of myoglobin in < 5 min from an 80% aceto

117 ety of multiply charged model peptides and a tryptic digest of myoglobin.

118 HPLC/MS analysis of a tryptic digest of NPR-ECD identified five glycosylated p

119 ctural analysis of the peptides of the total tryptic digest of oat phyA, we found that the photorecep

120 system was used to identify a peptide from a tryptic digest of ovalbumin using standard addition and

121 ution chromatogram of a fluorescently tagged tryptic digest of ovalbumin.

122 -containing peptides was demonstrated with a tryptic digest of ribonuclease A.

123 IMS-MS analysis was assessed using a complex tryptic digest of S. cerevisiae proteins.

124 The approach was tested on tryptic digest of Saccharomyces cerevisiae histones.

125 When applied to a global tryptic digest of Shewanella oneidensis proteins, an ord

126 selective enrichment of phosphopeptides from tryptic digest of standard protein (alpha-casein, beta-c

127 Mass spectrometric analysis of a tryptic digest of Stt3p showed that the peptide containi

128 LC/MS/MS analysis of a tryptic digest of the 51 kDa subunit from GS-NDH reveale

129 LC/MS/MS analysis of a tryptic digest of the 51-kDa polypeptide revealed that c

130 A tryptic digest of the bovine alpha-crystallin A chain yi

131 A tryptic digest of the ClAc-DMDDF-inactivated enzyme yiel

132 pparatus for complex mixtures, 5 microg of a tryptic digest of the cytosolic proteins of yeast was an

133 mance liquid chromatographic analysis of the tryptic digest of the HOCl-treated proteinase demonstrat

134 On the basis of complete mapping of total tryptic digest of the iodoacetamide-modified oat phytoch

135 HPLC, MS. and MSMS, and identified it in the tryptic digest of the ISC beta-actin.

136 -glycosylated peptide from an unfractionated tryptic digest of the lectin of the coral tree, Erythrin

137 Analysis of a tryptic digest of the main reversed phase-high pressure

138 strated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.

139 quencing of peptides obtained from an in-gel tryptic digest of the monomer and tetramer by tandem mas

140 Tryptic digest of the protein extracted from a sodium do

141 liquid chromatography/ESIMS analysis of the tryptic digest of the protein followed by subsequent mat

142 The MALDI MS analysis of a tryptic digest of the protein showed a number of potenti

143 demonstrated by applying 50 and 38 fmol of a tryptic digest of the proteins beta-lactoglobulin and bo

144 An in-gel tryptic digest of the purified protease was analyzed by

145 Mass spectrometry analysis of the tryptic digest of the reaction product indicated that so

146 Tandem mass spectroscopy of a tryptic digest of this 12.5 kDa protein identified it as

147 D LC-MS/MS) approach was used to analyze the tryptic digest of toxoid as a whole.

148 eparations of a model mixture of peptides, a tryptic digest of trypsinogen, and < 0.05% of an individ

149 version in TPI, which could be detected in a tryptic digest of tumor-derived TPI by mass spectrometry

150 We also subjected a tryptic digest of VV ATI to liquid chromatography electr

151 ll of reversed-phase C18 functionality) to a tryptic digest of whole Jurkat cell lysate to estimate t

152 protein digests of known concentrations, and tryptic digests of 2-DGE-separated proteins.

153 omparison of two-dimensional peptide maps of tryptic digests of 32P-labeled recombinant cPLA2 and hum

154 by high performance liquid chromatography of tryptic digests of 32P-labeled recombinant cPLA2 showed

155 Tryptic digests of 32P-phosphorylated and -immunoprecipi

156 Tryptic digests of [32P]PPK contain a predominant 32P-la

157 Tryptic digests of A1 and A2 subtypes of BoNT were analy

158 resent in the Gly(136)-Arg(147) peptide from tryptic digests of AGT reacted with DBE.

159 Comparison of tryptic digests of an HIV-1 IN derivative competent for

160 The method was applied to tryptic digests of beta-casein and alpha-casein.

161 Fluorescently labeled products from tryptic digests of beta-casein were analyzed in 13 min w

162 When analyzing tryptic digests of beta-casein, the Fe(III)-NTA-PHEMA br

163 I)-IMAC and ZrO2, was also carried out using tryptic digests of both simple and moderately complex pr

164 the abundances of phosphorylated peptides in tryptic digests of bovine beta-casein and protein kinase

165 Tryptic digests of bovine cytochrome c and beta-lactoglo

166 Analyses of tryptic digests of bovine serum albumin (BSA) by LC-MS-M

167 s pump to perform nanoflow HPLC separations; tryptic digests of bovine serum albumin (BSA), transferr

168 e signature 2-oxo-acid N-terminal peptide in tryptic digests of bronchoalveolar lavage fluid from pat

169 major phosphorylation peaks were detected in tryptic digests of cdb3 separated by reverse phase HPLC.

170 y 90% of tandem mass spectra identified from tryptic digests of complex protein mixtures.

171 In-solution tryptic digests of cooked meats were deposited onto a po

172 In CE separations performed on tryptic digests of dogfish myelin basic protein (MBP) wh

173 Tryptic digests of each band were analyzed by mass spect

174 Tryptic digests of fibrin that underwent differential cr

175 electivity of the approach are presented for tryptic digests of FP-biotinylated trypsin and FP-biotin

176 s spectrometry, a peptide (obtained from the tryptic digests of HOCl-treated cyt c) corresponding to

177 ionization mass spectrometry (ESI-LC/MS) of tryptic digests of human alphaB-crystallin in the presen

178 standard addition and to distinguish between tryptic digests of human and bovine hemoglobin.

179 Tryptic digests of human serum albumin and human lung ep

180 The SLAC strategy was applied to tryptic digests of human serum, and it was found that mo

181 uantitating, and annotating Cys34 adducts in tryptic digests of human serum/plasma.

182 ies or post-translational modifications from tryptic digests of individual proteins as well as whole

183 n sites after ablation of CypD, we subjected tryptic digests of isolated cardiac mitochondria from wi

184 Phosphopeptide analysis of tryptic digests of Lck from CD45- YAC-1 cells revealed t

185 e phosphorylation sites were detected in the tryptic digests of middle and C-terminal regions of IRS-

186 Alpha-ZrPN were applied for the analysis of tryptic digests of mouse liver and leukemia cell phospho

187 y nor two-dimensional phosphopeptide maps of tryptic digests of NHE3V.

188 e appearance of a phosphopeptide not seen in tryptic digests of p53 from untreated cells.

189 MALDI-TOF mass spectrometric analyses of tryptic digests of platelet-derived FV peptides detected

190 Tryptic digests of polyubiquitinated species contain mod

191 s was demonstrated with several peptides and tryptic digests of protein mixtures by LC-MS/MS experime

192 f LC-MALDI-TOF-MS and LC-ESI-TOF-MS data for tryptic digests of protein mixtures.

193 O2 in the enrichment of phosphopeptides from tryptic digests of protein mixtures.

194 Most peptides that are observed from tryptic digests of proteins such as cytochrome c and myo

195 The yielded tryptic digests of proteins were analysed by CZE in four

196 s of peptide mixtures such as those found in tryptic digests of proteins.

197 The device has been evaluated with the tryptic digests of proteins.

198 esorption ionization (MALDI) for analysis of tryptic digests of proteins.

199 Tryptic digests of purified recombinant mouse AChE (mACh

200 Tryptic digests of riPLA(2)beta (83 kDa) in the presence

201 The standard peptide mixtures and tryptic digests of samples of different origins were sep

202 with stable isotope coding of peptides from tryptic digests of serum.

203 f flight mass spectrometry was used to study tryptic digests of SHV-1 and S130Gbeta-lactamases (+/- i

204 Tryptic digests of the 150- and 135-kDa proteins yielded

205 LC/MS/MS analysis of tryptic digests of the 51 kDa and 75 kDa polypeptides fr

206 Mass spectrometric analysis of tryptic digests of the gamma-globins verified the amino

207 Peptide mapping of tryptic digests of the inactivated CYP2B6 using electros

208 ity and selectivity is demonstrated with the tryptic digests of the naturally phosphorylated proteins

209 et-Tyr-Trp cross-link (as probed by LC/MS on tryptic digests of the protein), exhibited any correlati

210 S) cross-linked precursors, derived from the tryptic digests of three model proteins (Human Serum Alb

211 od is demonstrated on a model peptide and on tryptic digests of three proteins.

212 echniques was applied to the analysis of the tryptic digests of three well-characterized protein mixt

213 tted the identification of 111 proteins from tryptic digests of total hair from AKR/J-hid/hid mice, w

214 k areas of the two transition fragments from tryptic digests of whey proteins in stored milk protein

215 deglycosylated and intact glycopeptides from tryptic digests of whole influenza virus, we determined

216 furosine results indicated that MRM based on tryptic digests of whole products was a feasible method

217 The method was evaluated using tryptic digests of yeast enolase and alcohol dehydrogena

218 r the gradient separation of peptides from a tryptic digest on a 27-cm-long capillary packed with 1.0

219 B was fully characterized using 100 fmol of tryptic digest on a three-dimensional ion trap mass spec

220 rithm to interpret sequence information of a tryptic digest on alpha-casein s1.

221 xtended now for the direct derivatization of tryptic digests originating from 1-5 microg of proteins

222 48 h in the presence or absence of a peptic-tryptic digest (P-T digest) of gliadin.

223 The comparison of tryptic digest patterns of free and vesicle-bound wild t

224 A 5-kDa tryptic digest peptide fragment containing six acidic re

225 to measure collision cross sections for 968 tryptic digest peptide ions obtained from digestion of c

226 illary columns for the LC-ESI-MS analysis of tryptic digest peptide mixtures.

227 ch is demonstrated by examining a mixture of tryptic digest peptides of ubiquitin.

228 was illustrated by analysis of a mixture of tryptic digest peptides using high- and low-resolution i

229 sing a mixture of cytochrome c and myoglobin tryptic digest peptides.

230 tified and quantified the abundance of 1,056 tryptic-digested peptides, representing 163 proteins in

231 Analysis of tryptic digests prepared from phosphorylated beta2-adren

232 muFFE analysis of a Chromeo P503-labeled BSA tryptic digest produced a 2D separation that made effect

233 mensional separation of bovine serum albumin tryptic digest produced a peak capacity of 4200 (110 in

234 rified using a set of commercially available tryptic digest protein standards analyzed using an ABI 4

235 In the initial stage, markers from tryptic digested protein of chilled, boiled and autoclav

236 d chromatography (LC)/mass spectrometry on a tryptic-digested protein sample.

237 o provide a simple extension of the existing tryptic digest protocols to include carbohydrate analysi

238 Peptide profiles of tryptic-digested recombinant protein and the purified ra

239 dataset, and up to 77% more spectra from non-tryptic digests, relative to a fully supervised approach

240 A partial tryptic digest revealed that, in the presence of agonist

241 ization-mass spectrometry analyses of SERCA1 tryptic digests revealed ca. 66% coverage of the protein

242 Mass spectrometric analysis of CPT-IL tryptic digests revealed the presence of three phosphope

243 MS/IMS/TOF performance for a protein mixture tryptic digest reveals high orthogonality between FAIMS

244 proteins were identified from a 50 ng in-gel tryptic digest sample combining five cuts in a single LC

245 nct S. oneidensis proteins from a 2.5-microg tryptic digest sample in a single 10-h analysis.

246 Shewanella oneidensis proteins from a 300-ng tryptic digest sample in a single 4-h LC-MS/MS analysis.

247 f the platform was evaluated using an in-gel tryptic digest sample of a gel fraction (15-40 kDa) of a

248 Mass analysis of in-gel tryptic digest samples provided 73% total sequence cover

249 en demonstrated using a bovine serum albumin tryptic digest separated by capillary LC where multiple

250 l 60 F(254S) plate and peptides from protein tryptic digests separated on a ProteoChrom HPTLC Silica

251 Mass spectrometry analysis of tryptic digests showed that Thr(1037) located within the

252 Isolation of the labeled peptide from the tryptic digest shows that Tyr(79) is the only enzymic am

253 The strategy includes periodate oxidation of tryptic digests, solid-phase enrichment of glycopeptides

254 omole amounts of crudely purified samples of tryptic digest solutions of horse cytochrome c and bovin

255 f small molecules and a bovine serum albumin tryptic digest, TASF improved the peak shape and resolut

256 Applied to a Saccharomyces cerevisiae tryptic digest, the approach provided 3 132 confident pe

257 e photolabeled PKCdelta C1B was subjected to tryptic digest, the fragments were separated by online c

258 For these two tryptic digests, the alpha-ZrPN approach is able to capt

259 When used with a tryptic digest, this gave unique information on only hal

260 anoLC-FAIMS-MS/MS of an unfractionated yeast tryptic digest using the modified FAIMS device identifie

261 xoid has been established by analysis of its tryptic digest using two-dimensional liquid chromatograp

262 Peptides were identified from tryptic digests using microsequencing by tandem mass spe

263 Immunoblotting analysis of Pgp tryptic digests using Pgp-specific NH(2)11, C219, and C4

264 A bovine serum albumin tryptic digest was also analyzed, and a sensitivity incr

265 glycosylated via an MS/MS experiment, so the tryptic digest was deglycosylated to confirm the presenc

266 e peptide 66LVNEVTEFAK75, also formed in the tryptic digest, was used as the native reference peptide

267 column that, on aminoterminal sequencing of tryptic digests, was identified as cytokeratin 1.

268 eline resolved, and peptides from an albumin tryptic digest were much better resolved than with exist

269 h-efficiency, two-dimensional separations of tryptic digests were achieved using glass microfluidic d

270 The tryptic digests were analyzed by LC-MS/MS, and tryptic p

271 9 and 12 amino acids that represent typical tryptic digests were designed, synthesized, and analyzed

272 The tryptic digests were passed over an immunoadsorbent spec

273 Tryptic digests were prepared in 50 mM formic acid and l

274 Meat tryptic digests were subjected to peptidomics analysis b

275 with trypsin in protonated solution, and the tryptic digests were then analyzed via liquid chromatogr

276 m fetuin, glycophorin A, ovalbumin and gp120 tryptic digests were used to build a spectral database o

277 t of casein phosphopeptides from a simulated tryptic digest with bovine serum albumin (BSA:casein, 10

278 ocol is based on glycopeptide selection from tryptic digests with serial lectin affinity chromatograp

279 e liquid chromatography (HPLC) analysis of a tryptic digest yielded an octapeptide within the insert