ライフサイエンスコーパス: tryptic digestion

1 ion of intact protein ions (i.e. without any tryptic digestion).

2 ydrodynamic radius measurements, and limited tryptic digestion.

3 t mass spectrometry (MALDI-TOF MS) following tryptic digestion.

4 ponding to p125 was excised and subjected to tryptic digestion.

5 borating this, tRNA(Glu) protected MnmA from tryptic digestion.

6 ated by the resistance of the heterodimer to tryptic digestion.

7 nd Anchym2 zymogens are activated by partial tryptic digestion.

8 ix individual spots were subjected to in-gel tryptic digestion.

9 turn structure and the stereoselectivity of tryptic digestion.

10 in this single structural domain protein by tryptic digestion.

11 aining the expected shortened fragment after tryptic digestion.

12 with the anticipated results of a simulated tryptic digestion.

13 hat can be robustly detected following rapid tryptic digestion.

14 BSA peptides were generated by tryptic digestion.

15 njunction with endoglycosidase treatment and tryptic digestion.

16 in comparison to the commonly used one-step tryptic digestion.

17 als from MTs and renders them susceptible to tryptic digestion.

18 uction products, which were stable following tryptic digestion.

19 of the complex peptide mixtures afforded by tryptic digestion.

20 nd CTP protect a 67 kDa fragment of Lon from tryptic digestion.

21 gel electrophoresis and subjected to in-gel tryptic digestion.

22 digestion or treated directly by in-solution tryptic digestion.

23 posed of neutral phospholipids, protect from tryptic digestion a small portion of the segment that co

24 cular species derived from trastuzumab after tryptic digestion: a stable signature peptide (FTISADTSK

25 ylamide gel electrophoresis and subjected to tryptic digestion and amino acid sequence analysis.

26 s extracted from gel slices and subjected to tryptic digestion and analysis by mass spectrometry.

27 erwise, the reverse reaction occurred during tryptic digestion and analysis.

28 onto a polymer surface, followed by in situ tryptic digestion and comparative analysis using DESI-MS

29 such as Cdc42 and histone II-AS, followed by tryptic digestion and comparative two-dimensional phosph

30 Here we describe simultaneous tryptic digestion and gradual unfolding of native protei

31 Tryptic digestion and liquid chromatography electrospray

32 in serum using affinity techniques prior to tryptic digestion and liquid chromatography-tandem mass

33 Subsequent tryptic digestion and liquid chromatography/tandem mass

34 apped protein adducts were then subjected to tryptic digestion and MS analyses.

35 on blocks lysine side chains, and subsequent tryptic digestion and N-terminal peptide derivatization

36 acetamide (IE-TFA) followed by an exhaustive tryptic digestion and on-line HPLC-UV-electrospray MS an

37 ]diisopropylfluorophosphate and subjected to tryptic digestion and partial amino acid sequencing.

38 cal cross-linking of the complex followed by tryptic digestion and peptide identification through mat

39 m the acetylation of Ser516 was confirmed by tryptic digestion and peptide mapping of COX-2 labeled w

40 Tryptic digestion and peptide mapping of COX-2 reacted w

41 rotein subunit was purified and subjected to tryptic digestion and peptide sequence analyses.

42 d according to a shotgun protocol, and after tryptic digestion and prefractionation using strong cati

43 e site at Asn 317 was obtained after limited tryptic digestion and RP-HPLC.

44 Tryptic digestion and subsequent protein sequencing of t

45 arching, selected proteins were subjected to tryptic digestion and the resulting mixtures were analyz

46 mal membranes, and are readily removed after tryptic digestion and therefore may be membrane inserted

47 autophosphorylation determined after partial tryptic digestion and two-dimensional peptide analysis w

48 ethods, including total amino acid analysis, tryptic digestion, and collision-induced dissociation-el

49 ltistep sample cleanup at the protein level, tryptic digestion, and isotope dilution mass spectrometr

50 atography of protein on cobalt-loaded beads, tryptic digestion, and MALDI MS analysis are performed i

51 ragments were isolated from cell surfaces by tryptic digestion, and partial amino-terminal amino acid

52 s include isolation of cross-linked species, tryptic digestion, and subsequent peptide sequencing.

53 n reactions, poly(C) binding assays, limited tryptic digestions, and the bicyclomycin inhibition kine

54 Using protection against tryptic digestion as a measure of the activated conforma

55 uction, and (iv) increased susceptibility to tryptic digestion as a result of conversion of negativel

56 DNEQ and delta-DSE actins protected S1 from tryptic digestion as well as the wild type and rabbit ac

57 use of a conformation-specific antibody and tryptic digestion assays, we showed that glycosylation a

58 f this method for sequencing peptides from a tryptic digestion at subpicomole levels and for identify

59 In contrast, tryptic digestions at Lys-61 and -68 occurred at the sam

60 only used in protein solubilization prior to tryptic digestion, but the presence of the DS(-) hampers

61 , ubiquitinated peptides were enriched after tryptic digestion by peptide immunoprecipitation using a

62 ioxygen-saturated buffer was investigated by tryptic digestion coupled mass spectrometry.

63 Upon tryptic digestion, cross-linked peptides were identified

64 Collectively, our kinetic as well as tryptic digestion data suggest that both nucleotide bind

65 The sensitivity of p72 to tryptic digestion differed considerably in the presence

66 In this work, tryptic digestion experiments were performed to detect t

67 Finally, tryptic digestion followed by analysis using MALDI TOF m

68 p- or down-regulated spots were subjected to tryptic digestion followed by identification using matri

69 Global tryptic digestion followed by liquid chromatographic/mas

70 We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tand

71 In-gel tryptic digestion followed by mass spectral fingerprinti

72 Using tryptic digestion followed by mass spectrometry and amin

73 ein accurate mass measurement, the other for tryptic digestion followed by MS and MS/MS analyses.

74 In-gel tryptic digestion followed by nano-liquid chromatography

75 Tryptic digestion, followed by MS, confirmed the presenc

76 This method involves optimization of in situ tryptic digestion, followed by reproducible and uniform

77 generated comparable or better results than tryptic digestion for protein identification.

78 erformance liquid chromatography to on-probe tryptic digestion for subsequent analyses by MALDI-TOF M

79 Using preparative SDS-PAGE, in-gel tryptic digestion, high pressure liquid chromatography,

80 Our new procedure involves tryptic digestion in aqueous solution buffered to pH 8-8

81 ing protein in 18O water (H218O) followed by tryptic digestion in regular water (H216O).

82 Limited tryptic digestion indicated that the central region of a

83 as enhanced after reduction, alkylation, and tryptic digestion, indicating that the three-dimensional

84 however, R172W protein was more sensitive to tryptic digestion, indicative of a change in protein con

85 Our results demonstrate that LFASP-based tryptic digestion is efficient, robust, reproducible, an

86 Tryptic digestion is followed by strong cation exchange

87 pL sample volume required for this on-column tryptic digestion is, to our knowledge, the smallest yet

88 After delipidation and tryptic digestion, mass spectrometry was used to identif

89 radigm of chemical cross-linking followed by tryptic digestion, mass spectrometry, and database searc

90 A special tryptic digestion method has been developed to facilitat

91 was identified, by using LC-MS/MS, from the tryptic digestion mixture of the reaction products of th

92 rometer has been applied for the analysis of tryptic digestion mixtures of the protein bovine serum a

93 Moreover, the tryptic digestion of a complex protein mixture in an org

94 The method used was based on tryptic digestion of a protein followed by quantificatio

95 ect on DNase I binding and the fast phase of tryptic digestion of actin.

96 e mixtures, including one generated from the tryptic digestion of alpha-casein.

97 In silico tryptic digestion of amaranth globulins was carried out

98 esalting steps, and accomplishes the reduced tryptic digestion of an IgG2 mAb in a mildly acidic cond

99 In an LC/MS/MS analysis of a tryptic digestion of an IgG2 monoclonal antibody, 1712 p

100 First, limited tryptic digestion of CKI epsilon produces a protease-res

101 Tryptic digestion of differentially labeled carbonic anh

102 n subfragment-1 (S1) ATPase activity nor the tryptic digestion of G-actin and F-actin at the Lys-61 a

103 Tryptic digestion of hCTR1 occurred within the cytoplasm

104 Tryptic digestion of HGXPRTase labeled with iodo[2-14C]a

105 dies of the peptide fragments resulting from tryptic digestion of KatG(M255I) confirmed the presence

106 Tryptic digestion of loop 1 slightly accelerated the rel

107 tion of mAbs with intact disulfide bonds and tryptic digestion of mAbs after reduction and alkylation

108 e proteome that relies on solubilization and tryptic digestion of membrane proteins in a buffer conta

109 followed by a single-tube solubilization and tryptic digestion of membrane proteins.

110 Tryptic digestion of MyBP-H yielded two peptides: approx

111 nd sequencing of phosphopeptides obtained by tryptic digestion of protein extracts from HeLa cells.

112 to dissociate ADA-drug bindings, followed by tryptic digestion of protein pellets and subsequent LC-M

113 of small molecules and peptides obtained by tryptic digestion of proteins and entire proteins.

114 em can realize rapid, efficient and reusable tryptic digestion of proteins by taking advantage of its

115 Improved in-solution tryptic digestion of proteins in terms of speed and pept

116 microsequencing of oligopeptides obtained by tryptic digestion of purified IscS was used to design a

117 buffered methanol-based solubilization, and tryptic digestion of purified plasma membrane is describ

118 tion of the peptide fragments resulting from tryptic digestion of recombinant Mycobacterium tuberculo

119 technique for protein separation, and in-gel tryptic digestion of resolved protein bands has enhanced

120 sidues (the longest peptide identified after tryptic digestion of S. oneidensis) with an average accu

121 This prediction was verified by tryptic digestion of SERT-expressing membranes: in the a

122 the determination of thiolic peptides after tryptic digestion of serum albumins from different speci

123 domain previously observed following limited tryptic digestion of spectrin protein.

124 The sequence of a peptide obtained after tryptic digestion of the 42-kDa protein allowed the spec

125 Analysis of labeled peptides generated by tryptic digestion of the affinity-labeled proteins ident

126 After in-solution tryptic digestion of the aqueous protein material extrac

127 alyzing conserved peptides, derived from the tryptic digestion of the B subunits.

128 ido-[alpha-32P]ATP into the 2-5A synthetase, tryptic digestion of the covalently 32P-labeled enzyme,

129 Tryptic digestion of the CYP3A4-[(3)H]ritonavir incubati

130 chromatography (IMAC), which was followed by tryptic digestion of the enriched sample and quantificat

131 itive FAB mass spectrometry after exhaustive tryptic digestion of the Er-ITC-modified Ca-ATPase.

132 Tryptic digestion of the H, L, and P polypeptide chains

133 Tryptic digestion of the heme-protein adduct and Edman s

134 e near the phosphorylation site and favoring tryptic digestion of the Lys536-Asn537 peptide bond.

135 ed as the position of the reactive ligand by tryptic digestion of the modified protein and by mutatio

136 Tryptic digestion of the protein yielded peptides that i

137 inding sites on RYR1 are destroyed by a mild tryptic digestion of the sarcoplasmic reticulum membrane

138 acid sequences from two peptides, derived by tryptic digestion of the structural subunit of one type

139 re, we report on the study of the effects of tryptic digestion of these loops on the motor and enzyma

140 Tryptic digestion of these oxidized proteins followed by

141 eparations for complex peptide mixtures from tryptic digestion of yeast cytosol fractions were obtain

142 n in a high boiling point solvent, and (iii) tryptic digestions of cytochrome c at 22 and 40 degrees

143 Tryptic digestions of myosin, which produced heavy merom

144 ermore, after the reduction, alkylation, and tryptic digestion, only 4 of a possible 25 asparagine re

145 h an orthogonal traceable method using total tryptic digestion, peptide separation, and isotope dilut

146 Carbamylation of proteins followed by tryptic digestion produced peptides similar to those exp

147 duced amino acid sequence with a celery M6PR tryptic digestion product.

148 Analysis of the tryptic digestion products by immunoblotting, N-terminal

149 Tryptic digestion products were sampled, detected, and i

150 odulin (either form), bound to RYR1 prior to tryptic digestion, protects both the apocalmodulin and C

151 ences in relative peptide concentrations for tryptic digestions ranging from 15 min to 48 h.

152 mization of the conditions of extraction and tryptic digestion, restructured meat and blank values (t

153 s were generated based on a previous limited tryptic digestion result and hydrogen-deuterium exchange

154 otocol used in route A involved the steps of tryptic digestion, reversed-phase chromatographic fracti

155 otocol, which includes cell lysis, overnight tryptic digestion, sample analysis and database searchin

156 hosphorylation using a combination of in gel tryptic digestion, SDS-PAGE and HPLC, MALDI-TOF MS analy

157 by the Fe3O4@Al2O3 MNPs followed by on-plate tryptic digestion, selective enrichment, and MALDI-MS an

158 sis of this product by mass spectrometry and tryptic digestion showed that it was a cyclic form of tr

159 n can be achieved using this newly developed tryptic digestion station.

160 ication of 10 pg/mL, while introduction of a tryptic digestion step, followed by quantification of a

161 ents extracted from the pyriform gland after tryptic digestion support the assertion that PySp1 repre

162 re used to show that after glycinylation and tryptic digestion, the mass spectrometric response from

163 After tryptic digestion, the peptides were analyzed using mass

164 We have used tryptic digestion to determine whether Ca(2+) can regula

165 After limited tryptic digestion to ensure the accessibility of only ex

166 the performance of "bottom-up" (in-solution tryptic digestion), "top-down" (intact protein fragmenta

167 On-membrane tryptic digestion using this method requires half the ti

168 Tryptic digestion was performed in a small volume (3 mic

169 The tryptic digestion was physiologically significant, corre

170 12F protein by nucleotide as protection from tryptic digestion was unchanged.

171 The results of the capture after tryptic digestion were analyzed by MALDI TOF MS.

172 Oxidized peptides generated by tryptic digestion were identified by electrospray-Fourie

173 olyzing succinimide in H(2)(18)O followed by tryptic digestion were used to label and identify the si

174 sis of a LLDGSSTEIR glycopeptide released by tryptic digestion, which carried two variant structures,

175 tecting a characteristic peptide produced by tryptic digestion with an LOD of 3 pM.

176 ow volume protein analysis strategy based on tryptic digestion within the tip of a 1.5-microm capilla

177 ids reformation of disulfide bonds to enable tryptic digestion without alkylation of cysteine residue

178 mide treatment of PSP, followed by prolonged tryptic digestion yielded predominantly a 14C-labeled te