ライフサイエンスコーパス: tryptic peptide

1 ning basic amino acid residues (for example, tryptic peptides).

2 using LC-MS/MS for detection of a selective tryptic peptide.

3 +0) to six amino acid residues (+6) for each tryptic peptide.

4 observed for arginine- and lysine-terminated tryptic peptides.

5 rified protein, and LC-MS/MS analysis of CRP tryptic peptides.

6 ne product by mass spectrometric analysis of tryptic peptides.

7 uorophore based on mass spectral analysis of tryptic peptides.

8 ibed for a collection of approximately 40000 tryptic peptides.

9 reaction with C-terminal digestion to modify tryptic peptides.

10 ntified in acidic fractions from analysis of tryptic peptides.

11 -spectrometric analysis of their constituent tryptic peptides.

12 terns for singly charged Arg- and Lys-ending tryptic peptides.

13 724-739) were identified as isoform-specific tryptic peptides.

14 n the intense y series peaks associated with tryptic peptides.

15 the ion current ratio determined for the two tryptic peptides.

16 f-flight mass spectrometry (MALDI-TOF MS) of tryptic peptides.

17 bearing characteristics very different from tryptic peptides.

18 identified as Pg by mass spectrometry of its tryptic peptides.

19 cluded an estimated approximately 98% of all tryptic peptides.

20 cid sequences that exactly match iPLA(2)beta tryptic peptides.

21 s(354) by HPLC and mass spectral analysis of tryptic peptides.

22 e-modified cysteines by mass spectrometry of tryptic peptides.

23 s to compare yields of numerous varieties of tryptic peptides.

24 tation and by mass spectroscopic analysis of tryptic peptides.

25 block both N-termini and lysine residues of tryptic peptides.

26 and more sequence information for the large tryptic peptides.

27 to enhance MALDI tandem mass spectrometry of tryptic peptides.

28 LIC models developed for complex mixtures of tryptic peptides.

29 -activated dissociation (CAD) spectra of the tryptic peptides.

30 ing site for an inhibitor located within the tryptic peptide (128)AACWWAGIK(136) was identified.

31 wing the formation of the bis-phosphorylated tryptic peptide, 173-HTDDEMT*GY*VATR-186, using [gamma-3

32 Two unique deglycosylated tryptic peptides, (21)EAENITTGCAEHCSLNENITVPDTK (45) (T

33 etylated amino groups and introduce into the tryptic peptide a biotin affinity handle, a visible moie

34 has been demonstrated with a triply charged tryptic peptide, a triply charged tryptic phosphopeptide

35 s been demonstrated with a doubly protonated tryptic peptide, a triply protonated phosphopeptide, and

36 Levels of the tryptic peptide ALFDFLK, found in the schizophrenia risk

37 oaches using selected reaction monitoring of tryptic peptides (also known as bottom up) have become c

38 d substrate ssDNA bind to the same three A3G tryptic peptides (amino acids 181-194, 314-320, and 345-

39 Incorporation of 5TFI was confirmed by tryptic peptide analysis and matrix-assisted laser desor

40 a combination of protein mass spectrometry, tryptic peptide analysis, and (32)P radiolabeling.

41 hout enrichment of glycopeptides from global tryptic peptides and at a false discovery rate of 1%, 10

42 as obtained using PACE-MSI for both digested tryptic peptides and endogenous neuropeptides from compl

43 sylation sites were separated from all other tryptic peptides and identified using MS data.

44 MS and MS/MS spectra of BR tryptic peptides and intact protein were generally super

45 tion, MALDI-ToF mass fingerprint analysis of tryptic peptides and MALDI-ToF reflectron in-source deca

46 By isolating the tryptic peptides and subjecting them to Edman sequence a

47 abundance by counting the number of detected tryptic peptides and their corresponding MS spectra.

48 sing mass spectra of the cysteine-containing tryptic peptides and used to follow the unfolding of eac

49 cal lengths (e.g. shorter/longer than common tryptic peptides) and lack of tryptic residues to facili

50 )(1%)) of 20.2 and 20.5, common sequences of tryptic peptides, and cross-reactivity with FAP antibody

51 atographic fractionation of the ICAT-labeled tryptic peptides, and protein identification and quantif

52 sotopic tags to both the N- and C-termini of tryptic peptides, and second, a search engine (based on

53 nal activation of modified lysine-terminated tryptic peptide anions is consistent with a covalent mod

54 in 50 min from approximately 4000 identified tryptic peptides; approximately 550 proteins in 20 min f

55 The effective peak capacities in analyses of tryptic peptides are approximately 500 for FAIMS/IMS sep

56 d y-ion series, and LC retention time of the tryptic peptides are essential pieces of information for

57 We hypothesize that non-tryptic peptides are mainly created from the truncation

58 r and, because cysteine residues on separate tryptic peptides are measured separately, is not complic

59 metry (ESI-tandem MS), a total of 6112 fully tryptic peptides are sequenced at a 1% false discovery r

60 Stable-isotope-labeled (tryptic) peptides are spiked into digested protein sampl

61 g synthetic heavy isotope-labeled C-terminal tryptic peptides as spiked standards with a triple quadr

62 ed multiple reaction monitoring quantitative tryptic peptide assays were developed for each phosphory

63 s generated containing 8400 MS/MS spectra of tryptic peptides assigned with high probability to an am

64 ed that phosphorylation occurred on a single tryptic peptide at Ser-174.

65 tein and the few hydrophobic and hydrophilic tryptic peptides available in the digest is demonstrated

66 s in the mass spectra of this doubly charged tryptic peptide, based solely on its amino acid sequence

67 ainly created from the truncation of regular tryptic peptides before separation.

68 responding increase in molecular mass of the tryptic peptide by 136 Da.

69 2SC formation were determined by analysis of tryptic peptides by high-performance liquid chromatograp

70 teins and direct detection of lipid-modified tryptic peptides by mass spectrometry.

71 sp. NRC-1 and identified the sequence of 23 tryptic peptides by nano-liquid chromatography electrosp

72 ry was performed on several of the resulting tryptic peptides by using collision quadrupole time of f

73 that the MALDI MS/MS analysis of sulfonated tryptic peptides can provide a highly effective method f

74 Stable isotopically labeled (SIL) tryptic peptides, cleavable SIL peptides, and a full-len

75 man degradation and liquid chromatography of tryptic peptides combined with tandem mass spectrometry

76 10-fold increase in the number of identified tryptic peptides compared to that obtained using a fixed

77 ed the mass distributions of all theoretical tryptic peptides composed of 20 natural amino acids and

78 ein concentration was shown to be unequal to tryptic peptide concentrations for most peptides, includ

79 crophages is phosphorylated on only a single tryptic peptide containing 14 potential phosphoacceptors

80 ptides, MALDI MS/MS analysis of a sulfonated tryptic peptide containing a diglycine branch generates

81 Further, the tryptic peptide containing the active center serine of A

82 ometry (LC-MS) with tandem MS (MS/MS) of the tryptic peptide containing the intramolecular disulfide

83 and asymmetric cleavage of disulfide-linked tryptic peptides containing Cys-99 and Cys-450.

84 his antibody only five were utilized, as the tryptic peptide derived from monoclonal IgM 12A1 contain

85 Mass spectral analysis of tryptic peptides derived from human ADAMTS13 indicate th

86 the analysis of coeluting model peptides and tryptic peptides derived from human plasma proteins, all

87 no acid pools in barley leaves and then into tryptic peptides derived from newly synthesized proteins

88 ptimize quantitation of different amounts of tryptic peptides derived from PE using light (H4, 4 hydr

89 upported by a mass loss of 2 Da observed for tryptic peptides derived from species that accumulate du

90 ometry to compare the relative abundances of tryptic peptides derived from suitable pairs of purified

91 When the mass defects of tryptic peptides derived from the human proteome are plo

92 LDI-TOF mass spectrometric fingerprinting of tryptic peptides derived from the purified NEC3 confirme

93 spray ionization-tandem mass spectrometry of tryptic peptides derived from whole cell digests.

94 pproach simplified the detection of glycated tryptic peptide elution in the LC/MS analysis by giving

95 CID as the unmodified cations for the small tryptic peptides examined here and more sequence informa

96 ities that are used to predict the number of tryptic peptides expected to be detected for one molecul

97 to analysis by MALDI-TOF mass spectrometry, tryptic peptide fingerprinting, molecular characterizati

98 ography tandem mass spectrometry analysis of tryptic peptides, followed by searching an appropriate s

99 ial QconCAT proteins that are concatamers of tryptic peptides for several proteins.

100 artificial proteins that are concatamers of tryptic peptides for several proteins.

101 ne strong cation exchange chromatography for tryptic peptide fractionation and combining it with the

102 Tryptic peptides fragment in ion trap tandem mass spectr

103 For the tryptic peptides, fragmentation is localized at the ends

104 intact proteins and/or an LC-MS analysis of tryptic peptide fragments generated after the oxidation

105 ectrometry/mass spectrometry analysis on the tryptic peptide fragments indicates that the 3-fluorosia

106 The tryptic peptide fragments of this phosphoprotein were se

107 proteins are generally characterized through tryptic peptide fragments, this paper reports a method f

108 onfirmed using the y14 ion and b8 ion of the tryptic peptide from bovine Hb alpha chain residues 69-9

109 nfolded and deamidated ribonuclease A, and a tryptic peptide from calmodulin deamidated in its native

110 dues 69-90, and HML was quantified using the tryptic peptide from human Hb alpha chain residues 63-91

111 A tryptic peptide from Tm*146-TnI with a molecular mass of

112 eamidated tryptic peptide of cytochrome c, a tryptic peptide from unfolded and deamidated ribonucleas

113 fidence identifications of >12 000 different tryptic peptides from >2000 distinct Shewanella oneidens

114 ent sequencing approach, a collection of 266 tryptic peptides from 23 model proteins were analyzed an

115 In the analysis of 31 tryptic peptides from 4 model proteins, the algorithm id

116 Separating tryptic peptides from [(32)P]orthophosphate-labeled cell

117 Tryptic peptides from a 250-kDa immunoprecipitated prote

118 btained by partial sequencing of distinctive tryptic peptides from Bacillus spores via post-source de

119 Through a PACE separation, 46 tryptic peptides from bovine serum albumin and 150 putat

120 esulting peptide anions, as demonstrated for tryptic peptides from bovine serum albumin and Halobacte

121 y, was evaluated using a mixture composed of tryptic peptides from caseins, bovine serum albumin, and

122 ed deamidation half-life for three different tryptic peptides from collagen (I) ranged from 2000 to 6

123 proteomics analyses to confidently identify tryptic peptides from complex mixtures of proteins, as w

124 Tryptic peptides from each spot group were analyzed in d

125 s of samples of complex mixtures composed of tryptic peptides from human and mouse blood proteins usi

126 even (15)N-labeled QconCATs that cover seven tryptic peptides from human clusterin with a length of n

127 s illustrated by examination of a mixture of tryptic peptides from human hemoglobin.

128 samples containing approximately 100 mug of tryptic peptides from mouse cerebrocortical brain tissue

129 oteins isolated from the mutants showed that tryptic peptides from phycocyanin that included Asn72 we

130 on initiation factor that binds Met-tRNA(i), tryptic peptides from rabbit reticulocyte eIF2A were ana

131 gradation and mass spectrometric analyses of tryptic peptides from rat DSP-PG, along with substitutio

132 On mass spectrometric analysis of tryptic peptides from recombinant secreted human punctin

133 Mass spectrometric analysis of tryptic peptides from the cross-linked product revealed

134 ional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome.

135 By calculating the masses of all potential tryptic peptides from the human proteome, we show that r

136 five pH bumps were applied to elute E. coli tryptic peptides from the monolith, followed by analysis

137 LC-MALDI-TOF/TOF MS analysis of tryptic peptides from the SCX fractions of an E. coli ly

138 derivatives and the production of different tryptic peptides from the unmodified and modified versio

139 Analysis of tryptic peptides from various fractions further confirme

140 For this purpose, tryptic peptides from whole cell lysates were analyzed b

141 C) was demonstrated from as little as 25 mug tryptic peptides from whole cell lysates.

142 xamined as a means of separating mixtures of tryptic peptides (from myoglobin and hemoglobin).

143 Differential dimethyl labeling of tryptic peptides generated from the purified therapeutic

144 from 27 different peptides (22 of which are tryptic peptides) has been studied in a 3D quadrupole io

145 Modified arginine-terminated tryptic peptides have shown evidence of a covalent modif

146 This approach involves (18)O labeling of tryptic peptides, high-efficiency enrichment of cysteine

147 introduce the notion of truncatability of a tryptic peptide, i.e. the probability of the peptide to

148 ithin a searching time comparable to that of tryptic peptide identification.

149 E to the mobile phase of nLC/MS experiments, tryptic peptide identifications increased from 93 to 111

150 eries of epitope sequences concatenated with tryptic peptides in a single artificial protein to creat

151 sured both the quantity and kinetics of SP-B tryptic peptides in tracheal aspirate samples of symptom

152 All tryptic peptides including N-terminal, C-terminal, and m

153 g in mass from 400 to 3000 m/z) of model and tryptic peptides, including serine, threonine, and tyros

154 LC-ESMS analysis of tryptic peptides indicated that PEDF A and B exhibit dif

155 Even so, mass spectrometric analysis of tryptic peptides indicated that the HMM CYP3A was in mol

156 LC-MS analysis of nonreduced tryptic peptides indicated trisulfide bonds are associat

157 ated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without

158 157 nm photodissociation of a singly charged tryptic peptide ion (NWDAGFGR) showed that prompt photof

159 tructural changes that would be expected for tryptic peptide ions and are consistent with the experim

160 ss spectrometry (IMS-MS) study revealed that tryptic peptide ions containing a proline residue at the

161 ser desorption/ionization (AP-MALDI)-derived tryptic peptide ions have been subjected to ion/ion reac

162 roteomic analysis, yet the singly protonated tryptic peptide ions produced by MALDI are significantly

163 viously been shown that photodissociation of tryptic peptide ions with 157 nm light in a matrix-assis

164 For N-glycosylated tryptic peptides, IRMPD causes extensive cleavage of the

165 y antipeptide antibodies, wherein a specific tryptic peptide is selected as a stoichiometric represen

166 Searching for all potential non-tryptic peptides is, however, time consuming for shotgun

167 d amount(s) of standard peptides (PE-derived tryptic peptides) is necessary for high-quality linear q

168 Further analysis on tryptic peptides led us to conclude that a single glycos

169 to conventional tandem MS spectra of native tryptic peptides, MALDI MS/MS analysis of a sulfonated t

170 The tryptic peptide map (mass spectral analysis) revealed th

171 A tryptic peptide map identified two isoAsp-containing pep

172 Tryptic peptide mapping and MALDI-MS verify labeling at

173 Using tryptic peptide mapping and mutagenesis, we have identif

174 Tryptic peptide mapping and tandem mass sequencing were

175 Tryptic peptide mapping and tandem mass spectrometry of

176 romatography, were fully characterized using tryptic peptide mapping and tandem mass spectrometry.

177 n was modified minimally with methylglyoxal, tryptic peptide mapping indicated a hotspot of modificat

178 Tryptic peptide mapping showed that the CpcSU-dependent

179 s as a tool to increase sequence coverage in tryptic peptide mapping.

180 rom cation-exchange chromatography (CEX) and tryptic peptide maps generated with the new digestion me

181 of the extracted ion chromatograms from the tryptic peptide maps.

182 The most robust tryptic peptide marker in the validation was LTLGSALAAPQ

183 LN-KO and wild-type hearts were subjected to tryptic peptide mass fingerprinting for identification b

184 By tryptic peptide mass fingerprinting we identified 55 pro

185 N-terminal sequencing and tryptic peptide mass spectrometry fingerprint analysis d

186 esponded to peptides that were identified by tryptic peptide mass spectrometry fingerprint analysis o

187 We obtained a partial tryptic peptide/mass spectrometry analysis of membrane m

188 eptide analytes from both simple and complex tryptic peptide matrices using selected reaction monitor

189 Identifying more peptides, e.g. non-tryptic peptides, may increase the peptide coverage and

190 of the serum protein and indirectly with the tryptic peptides measured by MS.

191 ence in peptide assignments was achieved for tryptic peptides, measured by changes in DeltaCN and RSP

192 roscopy, separated protein fluorescence, and tryptic peptide modification in liquid chromatography-ta

193 For freebase guanidinated BSA tryptic peptides, more than 6-times the peptides were ob

194 Three cross-linked tryptic peptides (nucleopeptides) of hRPA70xdT(30) (T(43

195 substance P peptide, as well as a mixture of tryptic peptides obtained by enzymatic digestion of cyto

196 LC-MS/MS analysis of the tryptic peptides obtained from the in vitro ubiquitinate

197 tion in peptides from proteins; a deamidated tryptic peptide of cytochrome c, a tryptic peptide from

198 The tryptic peptide of mAChE that contains the active center

199 A tryptic peptide of Mrf1p, GGQHVNTTDSAVR, containing the

200 defined by the presence of the COOH-terminal tryptic peptide of the CgA precursor, corresponding to r

201 ometer and applied for the separation of the tryptic peptides of a six-protein mixture and for the pr

202 formance liquid chromatographic radiolabeled tryptic peptides of alkylated bC2GnT-M on C18 column.

203 Selected tryptic peptides of beta-Lg (ALPMHIR, LIVTQTMK and VLVLD

204 An analysis of the tryptic peptides of cytochrome c formed by both ESI and

205 the tested BGEs, the best resolution of the tryptic peptides of extracted proteins of the above thre

206 HPLC analysis of tryptic peptides of FSBA-modified enzyme revealed the pr

207 untargeted "adductomics" method detected 50 tryptic peptides of HSA, containing Cys34 and prominent

208 ay ionization time-of-flight analysis of the tryptic peptides of mononitrated lysozyme identified the

209 ds of known amounts of heavy isotope-labeled tryptic peptides of PE provided linear calibration plots

210 Selected resolved tryptic peptides of proteins were characterised by effec

211 rmance liquid chromatographic profile of the tryptic peptides of reduced-alkylated (35)S-labeled C2Gn

212 This strategy is demonstrated with the tryptic peptides of several model proteins, including tw

213 M receptor as shown by MALDI-TOF analysis of tryptic peptides of TbetaR-V purified from bovine liver.

214 Mass spectrometry of tryptic peptides of the 15 kDa polypeptide identified it

215 romatography-tandem mass spectrometry of the tryptic peptides of the extracted/digested HU protein.

216 MS method for the direct detection of unique tryptic peptides of the KPC protein in clinical bacteria

217 Three tryptic peptides of the protein were isolated and sequen

218 ass of 2601.2 Da that was not present in the tryptic peptides of Tm*146 or TnI was identified using H

219 ion concatamer (QconCAT) carrying prototypic tryptic peptides of UCH-L1 was used as an internal stand

220 Glutamate-containing tryptic peptides of wild-type CYP4B1 were found labeled

221 lyze an interference model system comprising tryptic peptides of yeast that we contaminated with huma

222 relate the migration of 32P- and 35S-labeled tryptic peptides of ZEBRA.

223 times the peptides were observed relative to tryptic peptides or those guanidinated with the conventi

224 In PAL experiments with SIRT2, a tryptic peptide originating from the covalent attachment

225 exposure has been monitored by the ratio of tryptic peptide peaks that correspond to unmodified (uni

226 In the analysis of various tryptic peptides, photodissociation provided much more s

227 oteome level by examining the quality of the tryptic peptides prepared by on-surface nanodiamond dige

228 Amino acid analysis and Edman degradation of tryptic peptides proved that the core protein for DMP1-P

229 suitable for dissociating singly protonated tryptic peptides, providing greater sequence coverage th

230 he LC/MS/MS analysis of the affinity-labeled tryptic peptides purified from HPLC, identified two majo

231 artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gall

232 spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical

233 A control tryptic peptide, representing an unmodified region of FA

234 ging from 2.4- to 12.3-fold for the detected tryptic peptides resulted; the varying response was attr

235 Extensive sequencing of tryptic peptides revealed that the 37K fragments origina

236 Microsequencing of its two tryptic peptides revealed two perfect matches with the T

237 of 1465 ion trap spectra from doubly charged tryptic peptides reveals several trends important to und

238 highest truncatability are retained for non-tryptic peptide searching.

239 roteins were then characterized by SDS-PAGE, tryptic peptide sequence analysis, and Western blot anal

240 This approach enables the invariant tryptic peptide sequences within the family to serve as

241 nd liquid chromatography (LC)-MS analysis of tryptic peptides showed that 54 peptides distributed thr

242 yptic digests were analyzed by LC-MS/MS, and tryptic peptides specific for bovine and human Hbs were

243 Hb, and equine Hb were compared, and unique tryptic peptides specific for bovine Hb, human Hb, and e

244 single artificial protein to create internal tryptic peptide standards for MS as well as an intact pr

245 by mass spectrometric analysis of protonated tryptic peptides subjected to collisional activation.

246 nd MeArg were not found together on the same tryptic peptide, suggesting reciprocal regulation of the

247 was helpful to elucidating sequences of the tryptic peptides thanks to the fragment peptide ions pro

248 itration site was determined by sequencing a tryptic peptide that included Tyr23 and Tyr20, but possi

249 ad a retention time identical to that of the tryptic peptide that includes phycocyanobilin-bound, cys

250 APP-QconCAT includes tryptic peptides that are common for all isoforms of APP

251 all isoforms of APP concatenated with those tryptic peptides that are unique for specific APP isofor

252 For the tryptic peptide, the sequence is identified with more ce

253 ause, in contrast to the more widely studied tryptic peptides, the amino acids H, K, and R were posit

254 lision Induced Dissociation (CID) spectra of tryptic peptides], their performance often deteriorates

255 creased the sequence coverage for an average tryptic peptide to 92%.

256 ion and tandem mass spectrometry analysis of tryptic peptides to identify the PAL-E antigen as a secr

257 e, and laser desorption mass spectrometry of tryptic peptides, to define a series of epidermal protei

258 s studied by analyzing the separation of the tryptic peptides under different average column pressure

259 es were driven by the spatial orientation of tryptic peptides upon interaction with the negatively ch

260 igestion, online enrichment of IL-21 derived tryptic peptides using antipeptide antibodies, and quant

261 eptides in addition to a complex mixtures of tryptic peptides using LC-MS/MS, showing not only that A

262 digestion and quantitation of a NGF-derived tryptic peptide via high-flow peptide immunoaffinity enr

263 A modified tryptic peptide was characterized with an increase in ma

264 A unique 16-mer tryptic peptide was identified by conducting capillary L

265 This tryptic peptide was quantified using a synthetic peptide

266 A large-scale analysis of 755 doubly charged tryptic peptides was conducted to compare the method (ET

267 nd experimental m/z values for serum albumin tryptic peptides was found to be 8 ppm using the double

268 A limited set of tryptic peptides was generated in situ following selecti

269 The retention behavior of these tryptic peptides was measured under isocratic conditions

270 In addition, in-ESI-source fragmentation of tryptic peptides was observed in this study.

271 Separation of tryptic peptides was realized using a MicroHPLC interfac

272 s spectrometry and de novo sequencing of CPO tryptic peptides, we determined that three of the seven

273 nown histone H3/H4 acetylated and methylated tryptic peptides, we identified novel H3 K18 methylation

274 plying this method to identification of semi-tryptic peptides, we show that a significant number of s

275 ated measurements for each of the endogenous tryptic peptides were 17.0, 25.4, 24.2, and 14.0% for co

276 sone mesylate and the dexamethasone-modified tryptic peptides were analyzed by mass spectrometry, and

277 When tryptic peptides were analyzed by matrix-assisted laser-

278 motifs in interacting proteins, their unique tryptic peptides were analyzed by the motif scan softwar

279 Tryptic peptides were analyzed using Thermo linear ion-t

280 The tryptic peptides were chromatographically separated with

281 The amino acid sequences of these unique tryptic peptides were confirmed by their characteristic

282 only 3 x 108 cells, at least four different tryptic peptides were detected for each of 404 proteins

283 l peptide separations, a total of 6866 fully tryptic peptides were detected, leading to the identific

284 by one-dimensional gel electrophoresis, and tryptic peptides were extracted from gel slices and anal

285 In this study, tryptic peptides were generated in situ from complex spo

286 protein was purified to homogeneity, and its tryptic peptides were identified as gene At1g73080, whic

287 excised, and Ala-/Gly-rich, allele-specific tryptic peptides were identified by liquid chromatograph

288 ristics of these standards and collection of tryptic peptides were mapped into hydrophobicity index (

289 cted N-glycosylated peptides, 97% of oatp1a1 tryptic peptides were observed.

290 The biotinylated tryptic peptides were purified on a streptavidin affinit

291 were digested, and evolutionarily conserved tryptic peptides were quantified using isotope-dilution

292 purified and digested with trypsin, and the tryptic peptides were separated by high pressure liquid

293 lated by denaturing gel electrophoresis, and tryptic peptides were then subjected to affinity purific

294 e determined based upon the concentration of tryptic peptides, which in turn had been quantified base

295 age intensities of spiked synthetic catalase tryptic peptides, which we used as an internal standard,

296 Derivatization of tryptic peptides with another isothiocyanate analogue, 4

297 reliably detected based on identification of tryptic peptides with masses of 1148.59 Da, 1162.60 Da,

298 This method shows generation of tryptic peptides with sequence coverage up to 90% within

299 ction; (iii) enrichment of the biotin-tagged tryptic peptides with streptavidin; (iv) liquid chromato

300 After filtering, only a limited number of tryptic peptides with the highest truncatability are ret