ライフサイエンスコーパス: two-color

1 ue, or nearly dark, thus alternating between two colors.

2 e generalized to colocalization of more than two colors.

3 from the fluorescence intensity ratio of the two colors.

4 primers 5'-labeled with fluorescent dyes of two colors.

5 n microscopy imaging in three dimensions and two colors.

6 image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed a

7 t emission wavelengths, we also demonstrated two-color 3D super-resolution imaging in live cells.

8 lls encapsulated in collagen gels, obtaining two-color 4D super-resolution data sets spanning tens of

9 Together these two reagents enable two-color activation of neural spiking and downstream sy

10 sensory experience, performing simultaneous two-color activity recording, and applying optical pertu

11 ication of adjacent primer pairs, labeled in two colors, allowed us to narrow the 4q35.1 breakpoint t

12 Fluorescent two-color analysis of Gag-TC in HeLa cells revealed that

13 These methods are directly applicable to two-color antibody arrays.

14 essentially equivalent between the one- and two-color approaches and strongly suggest that this vari

15 compared with ten other body tissues using a two color array experimental design.

16 on is a promising method for the analysis of two color array experiments that compare multiple sample

17 We show how this improved the performance of two-color array analysis for arrays for genomic analysis

18 We describe a method for analyzing two-color array data to efficiently measure the differen

19 A two-color array of 20 excitation and deactivation beams

20 However, for data from two-color arrays, tests for differential expression may

21 ting different analysis methods designed for two-color arrays.

22 echnologies, such as single-color arrays and two-color arrays.

23 itu hybridization (PNA FISH) method, a rapid two-color assay for detection of C. albicans and C. glab

24 The second application is a two-color assay for the specific detection of the A-to-T

25 the two types of QD-probe conjugates in the two-color assay format is advantageous to maximize assay

26 s been combined with a cytokine Elispot in a two-color assay to confirm that cytotoxicity and interfe

27 at concentrations as low as 100 fM using the two-color assay.

28 4-fold increase in throughput over standard two-color assays.

29 Two-color Blink determined that different CTLs rarely oc

30 Our approach highlights how two-color calcium imaging can help identify and localize

31 ed for three-color whole-body imaging of the two-color cancer cells interacting with the GFP-expressi

32 ensitivity SHAPE experiments, we developed a two-color capillary electrophoresis approach with zeptom

33 large amount of research has been devoted to two-color cDNA arrays to improve experimental design, no

34 oligonucleotide arrays, each hybridized with two-color cDNA samples derived from 20 different human t

35 We report a two-color, cell-based screen to identify specific recept

36 learned a color change-detection task where two colored circles (selected from a 4-color set) were p

37 uorescence imaging readily distinguished the two color-coded cell lines and their different abilities

38 riate thresholds for the analysis of sets of two-color coincidence data.

39 sing a simple microfluidic device to perform two-color coincidence detection (TCCD) and single pair F

40 the method of single-molecule fluorescence, two-color coincidence detection (TCCD) to detect coincid

41 we have been able to employ single-molecule two-color coincidence detection (TCCD) to observe direct

42 Two-color coincidence detection is a single-molecule flu

43 listic diagnostic applications, we show that two-color coincidence detection of single-stranded DNA m

44 This method should allow the application of two-color coincidence detection to more complex biologic

45 es can be directly counted without employing two-color coincidence detection.

46 Examples of suitable dyes for such two-color colocalization include Oregon Green or Alexa F

47 each virus using three different colors and two-color combinatorial labeling.

48 Furthermore, a two-color competitive SDA format permits accurate quanti

49 m quantitative statistical colocalization on two-color confocal images, specifically of plant cells.

50 rescued photoreceptor cells was confirmed by two-color confocal immunofluorescence analysis using ant

51 Two-color confocal microscopy indicated that ErbB2 is co

52 Combining SaCas9 and SpCas9, we demonstrated two-color CRISPR imaging with the capability to resolve

53 we report the development of an alternative two-color CRISPR labeling method using only the well-cha

54 We have overcome this limitation by using two-color cytokine enzyme-linked immunospot assays and c

55 istics of the switch are characterized using two-color cytometry.

56 ll syncytia can be readily identified by the two-color cytoplasmic staining method.

57 flow cytometry to determine cell size or by two-color cytoplasmic staining of infected cell populati

58 d SIM setup that is fast enough to record 3D two-color datasets of living whole cells.

59 iments typically use either a one-color or a two-color design to measure mRNA abundance.

60 Langerhans using a microfluidic system with two-color detection.

61 ake monomeric PAmCherry1 a preferred tag for two-color diffraction-limited photoactivation imaging an

62 onucleotide complex and of its components by two-color digital fluorescence microscopy.

63 r low-status families, we exposed animals to two color discrimination problems, one with all monkeys

64 discriminate large T's from L's, or bisected two-color disks from their mirror images under the same

65 Two-color DNA microarray-based detection was demonstrate

66 For data from a two-color dye swap experiment, the one-sample test appea

67 dyes or tautomeric benzofluorescein class of two-color dyes.

68 We have now used two-color Elispots to reveal substantial numbers of prim

69 ounds surveyed, five displayed well-resolved two-color emission in solvents as nonpolar as hexane.

70 paired lymphocyte samples and amplified with two-color energy-transfer (ET) fluorescent primers speci

71 Using two-color evanescent field microscopy, we imaged the lip

72 luorescent proteins in different colors, and two-color evanescent-field microscopy was used to view s

73 different fluorophores in solution by using two-color excitation and detection of coincidence fluore

74 In the latter case, by using two color excitations, the conjugate could be simultaneo

75 A two-color experiment that made use of two different size

76 Two-color experiments show that LAT redistribution is st

77 proved algorithms for interpreting data from two-color experiments, where reference and test samples

78 fewer arrays) will perform as efficiently as two-color experiments.

79 This higher resolution, two-color extension of the cPALM technique will expand t

80 ddress these issues, we have developed a new two-color Fab labeling method that allows two samples to

81 These results demonstrate that two-color FFS is a powerful tool for measuring dynamic p

82 lanking regions of two human inversions, and two-color FISH analysis was performed on sperm from hete

83 Dual, two-color FISH staining revealed coinfection of numerous

84 In addition, a simulated high-throughput two-color FLISA positively identified those wells in a 3

85 nd IL-8, and their use in both one-color and two-color FLISAs is demonstrated.

86 Finally, with two-color flow cytometric analysis of intracellular cyto

87 Using two-color flow cytometric analysis of intracellular cyto

88 t 3 months posttransplant, was determined by two-color flow cytometric analysis of peripheral blood g

89 ercentage of donor T cells was quantified by two-color flow cytometric analysis using antibodies spec

90 eruginosa and serovar Typhi strains, we used two-color flow cytometry and confocal laser microscopy t

91 l repertoire was evaluated in chimeras using two-color flow cytometry and monoclonal antibodies direc

92 ecific KIR in the NK cell line YTS [2] and a two-color flow cytometry assay for conjugate formation,

93 luorescence of CaValpha2delta1, indicated by two-color flow cytometry assays and confocal imaging, an

94 cence of CaValpha2delta1 as characterized by two-color flow cytometry assays and confocal imaging; 2)

95 Two-color flow cytometry confirmed that YUSAC-2 cells tr

96 A two-color flow cytometry cross-match was performed in 92

97 Two-color flow cytometry of normal leukocytes showed tha

98 ans of enzyme-linked immunosorbent assay and two-color flow cytometry respectively.

99 To overcome these limitations, we used two-color flow cytometry to identify and select microvas

100 cells expressing RANKL on their surfaces by two-color flow cytometry using FITC-conjugated osteoprot

101 By two-color flow cytometry we have detected a significant

102 of CD32 were detected by mAb and analyzed by two-color flow cytometry.

103 We have developed an in vitro, two-color fluorescence assay to monitor kinetics of sing

104 al neurons and astrocytes were assessed in a two-color fluorescence assay.

105 th a semiconductor quantum dot (QD)-mediated two-color fluorescence coincidence detection scheme.

106 We have used two-color fluorescence coincidence detection to directly

107 In two-color fluorescence correlation spectroscopy (TCFCS),

108 , raster image correlation spectroscopy, and two-color fluorescence cross-correlation spectroscopy.

109 re achieved by utilizing a CE-LIF array with two-color fluorescence detection.

110 ADCP was measured by two-color fluorescence flow cytometry using PKH2 (green

111 receptor subtypes and their G-proteins using two-color fluorescence fluctuation spectroscopy (FFS) of

112 ip and clinical significance, we developed a two-color fluorescence in situ hybridization (FISH) tech

113 We used two-color fluorescence in situ hybridization and three-d

114 apex of amplification were used to develop a two-color fluorescence in situ hybridization assay and a

115 Two-color fluorescence in situ hybridization was consist

116 ecture of human kinetochores, we developed a two-color fluorescence light microscopy method that meas

117 Similar to two-color fluorescence measurements, multiple enzyme lab

118 Two-color fluorescence microscopy confirmed that both un

119 PIE-FCCS is a two-color fluorescence microscopy method that is directl

120 We use an in vivo two-color fluorescence microscopy technique to determine

121 , were quantified in a blinded fashion using two-color fluorescence microscopy.

122 Here we describe an improved two-color fluorescence nanoscopy technique that can achi

123 This assay requires analyzing two-color fluorescence patterns in yeast colonies, which

124 Binding affinity was quantified by two-color fluorescence ratio imaging of cells stained wi

125 labeled vesicles, were detected by measuring two-color fluorescence signals simultaneously.

126 ere observed, each exhibiting characteristic two-color fluorescence signatures: 1) primary fusion, in

127 A two-color fluorescence spectroscopic approach was utiliz

128 tiple CpG island loci was then detected in a two-color fluorescence system.

129 Our two-color fluorescence-activated cell sorting-based scre

130 efficiency, high-throughput and quantitative two-color fluorescence-activated cell sorting-based scre

131 g green fluorescent protein were isolated by two-color fluorescence-activated cell sorting.

132 h sensitivity positive/negative screening by two-color fluorescence-activated cell sorting.

133 Incorporation of two colored fluorescent probes in each of the components

134 n microarrays, we trained neural networks on two-color fluorescent detection of serum IgG binding and

135 We synthesized a two-color fluorescent dextran to measure ASL [K(+)], TAC

136 oli mRNAs at single-gene resolution by using two-color fluorescent DNA microarrays.

137 omplement to heat-inactivated serum, using a two-color fluorescent dye to distinguish live and dead c

138 We report a new two-color fluorescent imaging system to visualize the mo

139 gether or staggered by 1, 3, or 5 weeks, and two-color fluorescent in situ hybridization and molecula

140 ranslocation t(5:6)(G1;F2) was identified by two-color fluorescent in situ hybridization in all tumor

141 Two-color fluorescent in situ hybridization on dissociat

142 Elapsed time was measured with a two-color fluorescent marker gene that converts from exp

143 errations, which is commonly achieved with a two-color fluorescent oligonucleotide probe (TaqMan) des

144 We have developed a two-color fluorescent reporter for cellular assays of ex

145 We performed transposon mutagenesis of a two-color fluorescent reporter strain to identify new re

146 We have constructed two-color fluorescent reporter strains in which ompF and

147 A newly developed whole-mount, two-color fluorescent RNA in situ hybridization method,

148 A simple, two-color fluorescent sample labeling and detection stra

149 We have employed a two-colored fluorescent reporter system to isolate FHF a

150 The two colors from Alexa Fluor 532 and YOYO-3 were disperse

151 e faster and more accurate discrimination of two colors from different categories than two colors fro

152 of two colors from different categories than two colors from the same category, even when same- and d

153 Our approach is based on the expression of a two-color fusion protein in a vector that allows the ins

154 We developed a two-color genetic screen to identify Lrp mutations that

155 ection of circulating bacteria cells (CBCs), two-color gold and multilayer magnetic nanoparticles wit

156 ssay format, we demonstrate development of a two-color homogeneous electrophoretic immunoassay for co

157 esis and without byssogenesis were used in a two-color hybridization to reveal the gene differential

158 Using a two color imaging approach that allows tracking of singl

159 Using two-color imaging and fast extracellular solution change

160 pands the scope of biotin ligase labeling to two-color imaging and yeast-based applications.

161 pHuji plus SEP pair, we perform simultaneous two-color imaging of clathrin-mediated internalization o

162 Two-color imaging of epidermal growth factor (EGF) ligan

163 Two-color imaging of tethered vesicles with Myo2p, the G

164 The method is based on two-color imaging of the relaxation of the protein conce

165 We demonstrate a two-color imaging system with single-molecule resolution

166 s make PATagRFP an excellent protein tag for two-color imaging techniques, including conventional dif

167 Simultaneous two-color imaging was used to correlate changes in endos

168 to cell-based therapies for retinal disease, two-color imaging was used to localize transplanted cell

169 iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cel

170 Moreover, confocal microscopy combined with two color immunofluorescence showed co-localization of t

171 immunohistochemistry (CD45), and single and two-color immunofluorescence (CD3, MHC-II, CD45).

172 Frozen sections were stained by two-color immunofluorescence for C4d, type IV collagen a

173 ned antigen-coated particles, as revealed by two-color immunofluorescence microscopy, and these cells

174 Two-color immunofluorescence revealed that one-third of

175 for several latent and lytic transcripts and two-color immunofluorescence staining to analyze express

176 With two-color immunofluorescence, C4d was localized in basem

177 Two-color immunofluorescent labeling of TLR2 and cytoker

178 biocytin-labeled PNs were visualized with a two-color immunoperoxidase procedure using nickel-enhanc

179 gnificance of this innervation, a sequential two-color immunoperoxidase technique was used in the pre

180 Two-color in situ analysis revealed that almost all HS3S

181 By two-color in situ immunofluorescence, CD11b(+) myeloid D

182 Using two-color interphase fluorescence in situ hybridization

183 at the air-water interface using femtosecond two-color IR-pump/vibrational sum-frequency probe spectr

184 of about 10 s, evidenced by isomer-selective two-color (IR-IR) spectroscopy.

185 Here, we demonstrate a two-color irradiation scheme whereby initiating species

186 By merging two-color iSPIM with automated lineaging techniques we r

187 ltaneous comparison of results from one- and two-color labeling designs, using two independent RNA sa

188 Here we demonstrate specific and orthogonal two-color labeling of repetitive sequences in living hum

189 Using two-color labeling, colocalization of both components of

190 CM expression was determined by two-color labeling.

191 Colocalization of two-color labels using wide-field microscopy is a method

192 hollow waveguide using synthesized few-cycle two-color laser waveforms.

193 r, we present a novel assay scheme that uses two-color latex labels for rapid multiplex detection of

194 eneous mammalian samples using a ratiometric two-color lectin microarray approach.

195 Furthermore, using two-color, live-cell superresolution cross-correlation s

196 ifferential expression more efficiently than two-color loop designs.

197 e-color loop designs are more efficient than two-color loop designs.

198 by simulation and compared to several other two-color microarray designs.

199 Two-color microarray experiments in which an aliquot der

200 In two-color microarray experiments, well-known differences

201 llows users to find optimal loop designs for two-color microarray experiments.

202 A primary reason for using two-color microarrays is that the use of two samples lab

203 or microarrays and the ratio error model for two-color microarrays or ratios built from two single-co

204 Three-color microarrays, compared with two-color microarrays, can increase design efficiency an

205 correction of cross-channel correlation for two-color microarrays, identification of genes specific

206 Using two-color microarrays, we analyzed the transcriptomes of

207 tent of probe-specific dye and slide bias in two-color microarrays.

208 e edited region in the genome in between the two color moieties.

209 riment varying the relative frequency of the two color morphs of D. sambucina to test whether rare mo

210 The results demonstrate the utility of the two-color MRP1 construct for investigating ATP-binding c

211 Two-color MRP1 transport activity was normal, as shown b

212 y MRP1 structural dynamics, we engineered a "two-color MRP1" construct by fusing green fluorescent pr

213 In addition, we developed a two-color multiplex assay in which cells individually ex

214 used for the fast analysis of an eight-loci, two-color multiplex short tandem repeat (STR) system for

215 tion, we coupled our quenching approach to a two-color NA assay with correlated, two-color, single-mo

216 Herein we report a paper-based two-color oligonucleotide detection assay with tunable s

217 sion of both sensors in a single cell allows two-color optical control of transcription both in batch

218 aged biomolecules at 900 nm may allow facile two-color optical interrogation of bimodal signaling pat

219 The model is experimentally confirmed using "two-color" optical spin noise spectroscopy on a mixture

220 asy-to-use method to evaluate the quality of two-color (or more) fluorescence coincidence measurement

221 tes as high as 4 s in one color and 8.5 s in two colors over tens of time points.

222 Two-color PALM shows that the freely diffusible mitochon

223 Here, we report the use of two-color photoactivated localization microscopy (PALM)

224 super-resolution techniques such as one- and two-color photoactivated localization microscopy (PALM).

225 ility, we paired rsKame with PAmCherry1 in a two-color photoactivated localization microscopy imaging

226 We used single and two-color photoactivated localization microscopy to stud

227 One version of the two-color photoanode, based on an organic push-pull dye

228 t a new and versatile method for fabricating two-color photoanodes featuring spatially isolated chrom

229 We performed a two-color photon echo experiment on a bacterial reaction

230 Here, we show that a set of two-color photon-echo experiments performs quantum state

231 Working with a two-color platform, this study uses ten spike-in microar

232 wn prospectively, has not been available for two-color platforms.

233 ed "continuously red-photoactivatable green" two-color probe for live cell imaging.

234 ce in situ hybridization was performed using two-color probes for BCL6, MYC, IGH-BCL6, and del(6)(q22

235 A global analysis of two-color profiles, excited at 660 nm and probed at 5 nm

236 gene knockdown studies, as demonstrated in a two color proof-of-principle study with two biological t

237 of different AMPA receptor subunits by using two-color pulse-chase labeling.

238 y be drastically increased with an optimized two-color pulse.

239 Two-color pump-probe experiments employing a single exci

240 Two-color quenched probe pairs were then tested in the d

241 G1, S, and G2, while Fucci(SCA) permitted a two-color readout of G1 and S/G2.

242 ) at nascent endocytic sites was revealed by two-color real-time fluorescence microscopy; the type I

243 l is studied using DC slice ion imaging with two-color reduced-Doppler detection at 243 nm and 212 nm

244 This method provides an ability to evaluate two-color registration compatible with the scale of mole

245 Using a combination of two-color resonant two-photon ionization (2C-R2PI), lase

246 wn that the fingerprints labeled with one or two colors, resulting in 40-70 bands per clone, were ass

247 were probed in replicate experiment sets by two-color, rolling circle amplification on microarrays c

248 Drosophila relies on the comparison between two color-sensitive photoreceptors, R7 and R8.

249 the translocation step we have extended our two-color single molecule fluorescence coincidence metho

250 We applied two-color single quantum dot tracking to quantify recept

251 This analysis uses two-color single-frame images to provide a systems-level

252 We demonstrated two-color single-molecule imaging by observing the spati

253 imaging microscopy (FRET/FLIM) combined with two-color single-molecule tracking to study the effect o

254 ole-genome genotyping (WGG) assay based on a two-color, single-base extension (SBE), single-nucleotid

255 ach to a two-color NA assay with correlated, two-color, single-molecule fluorescence detection.

256 antifying Ags mixed in different ratios in a two-color, single-molecule-counting assay on a glass sli

257 evice, and measuring the extent of mixing of two colored solutions under different angles and at flow

258 Two-color spatio-temporal image cross-correlation spectr

259 The discussion focuses on two-color spotted cDNA microarrays, but many of the same

260 intralaboratory quality control protocol for two color, spotted microarrays was developed using cDNA

261 Two-color sptPALM imaging was demonstrated using several

262 Following training, the women were shown two colored squares and asked to choose the one with the

263 Two-color staining showed that those cells that were abl

264 bsorption bands in the visible region of the two colored states of the molecular switch.

265 We also find that bulk, two-color, static quenching FRET experiments are best su

266 ipants first learned to discriminate between two color stimuli, one paired with an electrical shock (

267 nderstand this process, we developed a novel two-color structured illumination microscopy with single

268 ectrally distinguishable probes, we obtained two-color super-resolution images of mitochondria and th

269 es also facilitate acquisition of live-cell, two-color, super-resolution images, expanding the utilit

270 As a result, we have achieved routine two-color superresolution imaging and single-molecule de

271 Two-color superresolution imaging demonstrated colocaliz

272 compatible with time-lapse and simultaneous two-color superresolution imaging in living cells.

273 Using two-color superresolution imaging, we observed that the

274 Two-color superresolution localization microscopy confir

275 teins in T and HeLa cells using quantitative two-color superresolution localization microscopy.

276 psin-Hsc70 coclusters are also visualized by two-color superresolution microscopy.

277 )C SWNT isotopes, we demonstrate multiplexed two-color SWNT Raman-based protein detection.

278 Using a two-color system to visualize pairs of chromosome loci i

279 for multiplex detection beyond the available two-color systems.

280 r the S-cone pathway (isolated using Stiles' two-color threshold method) for the same locations.

281 Using elegant two-color time-lapse imaging, the authors showed that mi

282 nd comove with KIF5B along axons revealed by two-color time-lapse imaging.

283 We examined the feasibility of using a two-color time-resolved detection scheme with microdevic

284 We report on the design and performance of a two-color, time-resolved detector for the acquisition of

285 However, two-color TIRF microscopy using fluorescent proteins fus

286 Furthermore, two-color TIRFM showed that Rab11a-mCherry was present i

287 Furthermore, two-color total internal reflection fluorescence (TIRF)

288 Here we image with two-color total internal reflection fluorescence microsc

289 mentation assays and direct visualization by two-color total internal reflection fluorescence microsc

290 munostaining of the endogenous proteins, and two-color total internal reflection fluorescence structu

291 plate was sequenced on the microchip using a two-color two-lifetime format with POP-6 as the sieving

292 base flipping using a microfluidic mixer and two-color two-photon (2c2p) fluorescence lifetime imagin

293 Using in vivo two-color two-photon imaging with genetically encoded ca

294 ced in a single capillary gel column using a two-color, two-lifetime format.

295 We used two-color, two-photon uncaging to fire and block action

296 The two colors were either from the same or different catego

297 Two colors were successively presented within a block of

298 A two-color Western blotting technique is used to determin

299 Russian speakers were faster to discriminate two colors when they fell into different linguistic cate

300 By means of two-color z-scan fluorescence correlation spectroscopy,