ライフサイエンスコーパス: two-hybrid system

1 raction of full-length PsIAA4 in vivo (yeast two-hybrid system).

2 as a novel DAT binding partner using a yeast two-hybrid system.

3 2)-Heremans-Schmid glycoprotein in the yeast two-hybrid system.

4 n in a membrane-bound, split-ubiquitin yeast two-hybrid system.

5 2-kDa alpha-zein when expressed in the yeast two-hybrid system.

6 anchor in MinD interactions, using the yeast two-hybrid system.

7 rosophila PP1c-binding proteins in the yeast two-hybrid system.

8 14), in the N terminus of BfpE using a yeast two-hybrid system.

9 e (mPNGase) were detected by using the yeast two-hybrid system.

10 in affinity chromatography and in the yeast two-hybrid system.

11 mer-dependent readout from an AcnB bacterial two-hybrid system.

12 wed by phenotypic screening based on a yeast two-hybrid system.

13 human cDNA library was screened in the yeast two-hybrid system.

14 interaction with both XRCC3 and RAD51B in a two-hybrid system.

15 f AbetaPP have been isolated using the yeast two-hybrid system.

16 o-terminal end of P. yoelii MSP-1 in a yeast two-hybrid system.

17 ated the interaction directly with the yeast two-hybrid system.

18 ins that interacted with p35 using the yeast two-hybrid system.

19 h each other and self-associate in the yeast two-hybrid system.

20 1 receptor-interacting domain in a mammalian two-hybrid system.

21 IFT complex was investigated using the yeast two-hybrid system.

22 g) of SV40 virus in a tetracycline-inducible two-hybrid system.

23 (IncG) was demonstrated by using a bacterial two-hybrid system.

24 VacA (termed p-33 and p-55) by using a yeast two-hybrid system.

25 trap were active when tested using the yeast two-hybrid system.

26 y to mediate heterodimerization in the yeast two-hybrid system.

27 aptured proteins was verified in a bacterial two-hybrid system.

28 library via a variant of the original yeast two-hybrid system.

29 e 4OHT-bound ERalpha conformation in a yeast two-hybrid system.

30 protein was investigated by using the yeast two-hybrid system.

31 of protein-protein interactions is the yeast two-hybrid system.

32 , as well as genetic tools such as the yeast two-hybrid system.

33 d MazF was also characterized with the yeast two-hybrid system.

34 n of full-length Qin and TLE1 in a mammalian two-hybrid system.

35 ctin was originally characterized by a yeast two-hybrid system.

36 tant protein interactions has been the yeast two-hybrid system.

37 to interact with wild-type p-55 in the yeast two-hybrid system.

38 and found to interact strongly in the yeast two-hybrid system.

39 interacted with GlnK and GlnKY51F in a yeast two-hybrid system.

40 al regulatory region of p100 using the yeast two-hybrid system.

41 (28) was demonstrated by utilizing the yeast two-hybrid system.

42 or receptor family, as the bait in the yeast two-hybrid system.

43 ular meshwork (TM) cells through a mammalian two-hybrid system.

44 g that the interaction was not unique to the two-hybrid system.

45 SKR-1, -2, -3, -7, -8, and -10 in the yeast two-hybrid system.

46 beta-, gamma-, and delta-zeins in the yeast two-hybrid system.

47 complementary DNA library by using the yeast two-hybrid system.

48 and p67(phox) was demonstrated using a yeast two-hybrid system.

49 eened for p35-interacting proteins using the two-hybrid system.

50 xpression library was screened using a yeast two-hybrid system.

51 ct interaction of FleN and FleQ in the yeast two-hybrid system.

52 eraction was initially found using the yeast two-hybrid system.

53 also detected with the yeast split-ubiquitin two-hybrid system.

54 eoB, as determined using the BACTH bacterial two-hybrid system.

55 ouse Rpgr(ORF15) was used as bait in a yeast two-hybrid system.

56 he Cdh1 substrate-binding protein in a yeast two-hybrid system.

57 n-protein interaction assays using the yeast two-hybrid system.

58 eracts with AtbZIP68 and AtGBF1 in the yeast two-hybrid system.

59 re, LIN-56 and LIN-15A interact in the yeast two-hybrid system.

60 Munc18-1 was also identified using the yeast two-hybrid system.

61 inal domain (LN) interactions in a bacterial two-hybrid system.

62 -immunoprecipitation assay and the mammalian two-hybrid system.

63 rfered with MzrA-EnvZ binding in a bacterial two-hybrid system.

64 ve for multimerization using a reverse yeast two-hybrid system.

65 ing a highly specific, high-throughput yeast two-hybrid system.

66 ng site, CudA forms a homodimer in the yeast two-hybrid system.

67 directly with the FANCD2 protein in a yeast two-hybrid system.

68 tein, and they interact with each other in a two-hybrid system.

69 loped a cost-effective high-throughput yeast two-hybrid system.

70 r Msi1p to associate with Cac1p in the yeast two-hybrid system.

71 nal Y6 cell cDNA library using the bacterial two-hybrid system.

72 d by using a stringent high-throughput yeast two-hybrid system.

73 he Z ring or interact with FtsZ in the yeast two-hybrid system.

74 effect on MarR interaction with TktA in the two-hybrid system.

75 riptional activation and expression in yeast two-hybrid systems.

76 Using a yeast two-hybrid system, 38 candidates interacting with TcPKAc

77 tein interaction assays done using the yeast two-hybrid system, 56 (approximately 17%) showed positiv

78 We report that in the yeast two-hybrid system a domain of U(S)3 essential for antiap

79 sent study we have isolated, using the yeast two-hybrid system, a 182 amino acid residue fragment of

80 s using reporter genes: a modified mammalian two-hybrid system, a bioluminescence resonance energy tr

81 We use the well studied yeast two-hybrid system adapted for mammalian cells and modify

82 A bacterial two-hybrid system allowed us to provide clear evidence f

83 and HPS4 do not interact directly in a yeast two-hybrid system, although HPS4 interacts with itself.

84 Using the yeast-two hybrid system and coprecipitation of recombinant pro

85 ated polypeptides using the Gal4-based yeast two-hybrid system and a cDNA library derived from a chon

86 Using the yeast (Saccharomyces cerevisiae) two-hybrid system and a potato (Solanum tuberosum) KNOX

87 Using the yeast two-hybrid system and a protein array membrane, we ident

88 Using the yeast two-hybrid system and affinity immobilization assays, we

89 vealed by the RNAi assays, we used the yeast two-hybrid system and an in vitro glutathione-S-transfer

90 Using the yeast two-hybrid system and bimolecular fluorescence complemen

91 the importin alpha protein family in a yeast two-hybrid system and by an in planta bimolecular fluore

92 32, both by genetic criteria using the yeast two-hybrid system and by biochemical criteria using the

93 action was confirmed in a conventional yeast two-hybrid system and by direct interaction between puri

94 In a novel application of the yeast two-hybrid system and by immunoprecipitation, we show he

95 To explain this process, we show by a yeast two-hybrid system and chemical cross-linking that the lu

96 Additional studies using a mammalian two-hybrid system and ChIP indicate that 2MD is also mor

97 Snf1 also interacts with Nrg1 in the two-hybrid system and co-immunoprecipitates with both Nr

98 We used the yeast two-hybrid system and co-immunoprecipitation analysis to

99 Mammalian two-hybrid system and co-immunoprecipitation assays both

100 tion between Rad52 and Rad59 using the yeast two-hybrid system and co-immunoprecipitation from yeast

101 Using the yeast two-hybrid system and co-immunoprecipitation methods, we

102 Using both the yeast two-hybrid system and coimmunoprecipitation assays, we c

103 were systematically mapped using a mammalian two-hybrid system and confirmed using a co-immunoprecipi

104 ion was initially demonstrated using a yeast two-hybrid system and corroborated by both in vivo and i

105 ed out in Escherichia coli using a bacterial two-hybrid system and do not require specialized equipme

106 ha12.1 subunit by using a modified mammalian two-hybrid system and fluorescence resonance energy tran

107 reened a prostate cDNA library using a yeast two-hybrid system and found that the cleavage and polyad

108 a binding partner for UNC5H1 using the yeast two-hybrid system and found that the extreme three C-ter

109 decorin and EGFR was confirmed in the yeast two-hybrid system and further validated by experiments u

110 We used the yeast two-hybrid system and gel overlays to study intimin-Tir

111 eins was shown using a split ubiquitin yeast two-hybrid system and gel shift assays.

112 By using a bacterial two-hybrid system and genetic mutagenesis, we showed tha

113 We utilized a modified yeast two-hybrid system and identified a new, widely expressed

114 e cytoplasmic domain of ADAM12 using a yeast two-hybrid system and identified a protein called PACSIN

115 Nck via the intermediate domain in the yeast two-hybrid system and in a glutathione S-transferase (GS

116 pamine D(2) receptor interact in a bacterial two-hybrid system and in a poly-histidine pull-down assa

117 uired for interaction with skNAC in both the two-hybrid system and in coimmunoprecipitation experimen

118 interacts physically with eIF4A in the yeast two-hybrid system and in GST pull-down assays and that t

119 with Agrobacterium protein VirE2 in a yeast two-hybrid system and in planta.

120 terminal motif in Hook proteins in the yeast two-hybrid system and in tissue culture cells, and Hook

121 also interacts with OASTL based on the yeast two-hybrid system and in vitro binding assays.

122 t directly associates with maspin in a yeast two-hybrid system and in vitro.

123 ly developed interaction assays (e.g., yeast two-hybrid system and split-ubiquitin assay) usually are

124 of maspin, we employed a maspin-baited yeast two-hybrid system and subsequently identified Interferon

125 eins that interacted with INSM1 by the yeast two-hybrid system and the binding of one of them, Cbl-as

126 We employed the yeast two-hybrid system and used perlecan domain V as bait to

127 kigamma2, -gamma3, and -epsilon in the yeast two-hybrid system, and bound Ckidelta and -epsilon in pu

128 luoro-octanoate-PAGE, a membrane-based yeast two-hybrid system, and chemical cross-linking experiment

129 3DX bound well to its epitope in a yeast two-hybrid system, and GST-fused 3DX also bound to sever

130 plant SnRK AKIN11 both in vivo in the yeast two-hybrid system, and in vitro in a GST-fusion 'pull do

131 the spliceosome protein U1-70K in the yeast two-hybrid system, and is co-localized with U1-70K in nu

132 n cultured TM cells, by means of a mammalian two-hybrid system, and through biochemical coimmunopreci

133 Whereas a variety of two-hybrid systems are available to measure the interact

134 protein complexes, was identified by a yeast two-hybrid system as a strong and specific binding partn

135 DCL3, also known as PhLP2A), through a yeast two-hybrid system, as a novel protein involved in the st

136 s among lens crystallins in a mammalian cell two-hybrid system assay and speculated about the signifi

137 n complex in vivo as determined by bacterial two-hybrid system assay.

138 ein interactions was screened by a mammalian two-hybrid system assay.

139 Analysis of the Pnn motifs using two-hybrid system assays demonstrated that the polyserin

140 In a yeast two-hybrid system based on reconstitution of Ras signali

141 Immunoprecipitation and the yeast two-hybrid system both suggest physical interaction of t

142 Using the yeast two-hybrid system, CCTeta was found to bind to the N-ter

143 With a mammalian two-hybrid system, coimmunoprecipitation experiments, an

144 studied using affinity chromatography, yeast two-hybrid system, coimmunoprecipitation, and gel overla

145 purification-mass spectrometry or the yeast two-hybrid system, contributes a unique and relevant per

146 We used the yeast two-hybrid system coupled with random mutagenesis to ide

147 The yeast two-hybrid system data also indicated that CarR is capab

148 e, extensive assays utilizing the Gal4 yeast two-hybrid system demonstrate interactions of synemin wi

149 Experiments using the yeast two-hybrid system demonstrated a protein-protein interac

150 Use of the yeast two-hybrid system demonstrated direct interaction betwee

151 Analysis with a bacterial two-hybrid system designed to facilitate the study of pr

152 In this review we will introduce the yeast two-hybrid system, discuss modifications of the system t

153 library carrying M. xanthus DNA in the yeast two-hybrid system, eight positive, independent clones co

154 The bacterial two-hybrid system established a specific interaction bet

155 brane-based yeast (Saccharomyces cerevisiae) two-hybrid system established that tetraspanins can phys

156 t epithelial cell cDNA library using a yeast two-hybrid system for ARHI-interacting proteins.

157 brary was then introduced into yeast surface two-hybrid system for final quantitative selection of an

158 When these variants were tested in the two-hybrid system for their interaction with NtrB, a rec

159 A bacterial two-hybrid system further indicated that Rv3789 interact

160 its original description in 1989, the yeast two-hybrid system has been extensively used to identify

161 In the membrane-based yeast two-hybrid system, homo-oligomeric interactions between

162 at occur in E. coli also occurs in the yeast two-hybrid system (i.e., off-DNA).

163 obunyavirus N protein by yeast and mammalian two-hybrid systems, immunoprecipitation experiments, and

164 domains of each immune receptor in the yeast two-hybrid system in a kinase activity-dependent manner.

165 d using the yeast (Saccharomyces cerevisiae) two-hybrid system in Arabidopsis (Arabidopsis thaliana).

166 human DNA ligase IV, we have used the yeast two-hybrid system in conjunction with traditional bioche

167 have been combined with a bacterial reverse two-hybrid system in our labs and used in the identifica

168 We used a mammalian two-hybrid system in this study.

169 ein for readout of a tetracycline-inducible, two-hybrid system in vivo.

170 We demonstrate a two-hybrid system in which MAL62p is used in conjunction

171 By using the yeast two-hybrid system, in vitro coimmunoprecipitation, and i

172 Additional mapping studies in the yeast two-hybrid system indicated that only the N-terminal por

173 The yeast two-hybrid system indicated that regions near the C term

174 A Bacterial Two Hybrid system indicates that DauA and the sensor com

175 Analysis in the yeast two-hybrid system indicates that the N-terminal portions

176 EC proteins can dimerize with SPT in a yeast two-hybrid system, indicating that the HEC genes work in

177 criptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-

178 cells as demonstrated in yeast and mammalian two-hybrid systems; interaction sites are mapped to 237-

179 Using a bacterial two-hybrid system, it could be shown that the N-terminus

180 is sensitivity issue, we introduced in vitro two-hybrid system (IVT2H) into microfluidic drops and de

181 r-binding protein PspF to create an in vitro two-hybrid system (IVT2H), capable of carrying out gene

182 es the interaction with Rad54 protein in the two-hybrid system, leads to increased sensitivity to the

183 y-capture complex purification and the yeast two-hybrid system, may produce inaccurate data sets owin

184 d the Sos recruitment system, an alternative two-hybrid system method to detect protein-protein inter

185 When tested in the yeast two-hybrid system, mutation of Leu-536 increased the bas

186 riments using either a split ubiquitin yeast two-hybrid system or bimolecular fluorescence complement

187 e1 did not interact with ABIN-2 in the yeast two-hybrid system or mammalian cells.

188 on pathways have successfully used the yeast two-hybrid system or related methods.

189 ing the C-terminus of LPP as bait in a yeast two hybrid system, palladin, an actin-associated protein

190 Using a modified yeast two-hybrid system, PDZ(Omi) mutants were isolated by the

191 By employing a bacterial two hybrid system, pull down assays and surface plasmon

192 By using the yeast two-hybrid system, purified lectin and pilin domains, an

193 hance N-terminal dimerization in a bacterial two-hybrid system reconstituted in V. cholerae, which is

194 These data demonstrate that the imaging two-hybrid system responds in a proportional fashion to

195 ur approach increased the sensitivity of the two-hybrid system, resulting in a more complete interact

196 screen a human heart cDNA library in a yeast two-hybrid system, retrieving two unique clones that enc

197 Deletion analysis using the yeast two-hybrid system revealed that the armadillo repeat dom

198 opamine D(2) receptor as bait in a bacterial two-hybrid system, S100B was determined to be a potentia

199 A bacterial two-hybrid system screen identified bacterioferritins an

200 Although the yeast two-hybrid system suggested an interaction of six differ

201 A mammalian two-hybrid system (termed as trM2H) was developed to det

202 S could not be demonstrated with a bacterial two-hybrid system that reconstitutes an intracellular ce

203 er, MA showed binding to TSG101 in the yeast two-hybrid system that was dependent on an intact PTAP m

204 Utilizing a split-ubiquitin membrane yeast two-hybrid system that was developed to identify interac

205 In yeast two-hybrid systems, the N-terminus of NIL-16 interacts w

206 ort the application of an enhanced Dual Bait two-hybrid system to allow detection and manipulation of

207 We used the yeast two-hybrid system to analyze the role of Alpharetrovirus

208 graphy, coimmunoprecipitation, and the yeast two-hybrid system to demonstrate that the extracellular

209 We then used a yeast two-hybrid system to detect potential protein-protein in

210 We have used the yeast two-hybrid system to detect variants of GlnB that intera

211 ttaci, Texas turkey, were also cloned in the two-hybrid system to determine if LcrH-2 and LcrE would

212 In this study, we used the yeast two-hybrid system to determine the multimerization domai

213 Using the yeast two-hybrid system to determine the target host cell prot

214 We have modified the yeast two-hybrid system to enable the detection of protein-pro

215 llular signaling, we have modified the yeast two-hybrid system to explore the possibility of NO-depen

216 In this study we used the yeast two-hybrid system to find proteins interacting with scie

217 Here we present a two-hybrid system to follow the heterodimerization of me

218 We used a yeast two-hybrid system to identify a putative cotton fiber me

219 Here we have used a bacterial two-hybrid system to identify and quantify the interacti

220 We used the yeast two-hybrid system to identify binding partners of Lsm11

221 Here we use a yeast two-hybrid system to identify novel TIR1 mutants with al

222 Here, as a test case, we used a two-bait two-hybrid system to identify peptide aptamers that dist

223 Use of the yeast two-hybrid system to identify proteins that associate wi

224 P processing and function, we used the yeast two-hybrid system to identify proteins that interact wit

225 We used the yeast two-hybrid system to identify proteins that interact wit

226 transduction pathway, we have used the yeast two-hybrid system to identify proteins that physically i

227 We used the yeast two-hybrid system to identify the Prtb (Proline-rich tra

228 tegrated strategy based on the reverse yeast two-hybrid system to isolate and characterize such edge-

229 In this study, we have used the bacterial two-hybrid system to isolate cDNA-encoding proteins that

230 Because of the failure of the yeast two-hybrid system to reveal interactions between luminal

231 rgets of c-Abl kinase, we utilized the yeast two-hybrid system to screen a murine cDNA library.

232 Using the era gene as bait in the yeast two-hybrid system to screen E. coli genomic libraries, w

233 We developed a sensitive yeast two-hybrid system to screen for hERalpha variants with i

234 we used the yeast (Saccharomyces cerevisiae) two-hybrid system to screen for NF-YC1-interacting prote

235 horylation, we modified the Gal4-based yeast two-hybrid system to screen for phosphorylation-dependen

236 CSG1 in breast cancer cells, we used a yeast two-hybrid system to screen for proteins that could asso

237 We used an optimized yeast two-hybrid system to screen mouse pregnancy-associated l

238 Here, using a yeast two-hybrid system to search for AtRALF1-interacting prot

239 us, we screened a cDNA library using a yeast two-hybrid system to search for interacting protein(s) a

240 ts also demonstrate the utility of the yeast two-hybrid system to study protein-protein interactions

241 Here we have used the yeast two-hybrid system to test for direct interaction between

242 we screened a human cDNA library by a yeast two-hybrid system using IGFBP-5 as bait and identified f

243 In the present study, we employed yeast two-hybrid system using the N-terminal domain of AIP1 as

244 microvascular endothelial cells by the yeast two-hybrid system using the N-terminal domain of CNF1 as

245 stallin genes were cloned and fused into the two-hybrid system vectors (target and prey proteins).

246 The yeast two hybrid system was used to confirm these interactions

247 le protein interaction analyses, a bacterial two-hybrid system was coupled with a whole genome shotgu

248 le of RGSZ1 in cellular signaling, the yeast two-hybrid system was employed to identify potential eff

249 functional genetic screen based on the yeast two-hybrid system was performed.

250 A mammalian two-hybrid system was used to assay the protein-protein

251 Yeast two-hybrid system was used to demonstrate that a homodim

252 expression in single yeast cells carrying a two-hybrid system was used to detect in vivo protein-pro

253 In addition, a yeast two-hybrid system was used to detect the interactions of

254 The yeast two-hybrid system was used to identify a groucho homolog

255 t-ubiquitin yeast (Saccharomyces cerevisiae) two-hybrid system was used to identify B-cell-associated

256 The yeast two-hybrid system was used to isolate the ATP binding pr

257 The yeast two-hybrid system was used to search for proteins that b

258 A LexA-based two-hybrid system was utilized to define interaction dom

259 s interaction, first observed with the yeast two-hybrid system, was corroborated by co-immunoprecipit

260 Using the yeast-two hybrid system we isolated a novel Numb interactor in

261 Using a reverse two-hybrid system we previously identified mutations in

262 Furthermore, using a mammalian two-hybrid system, we confirmed that ARA55 interacts wit

263 Using the yeast two-hybrid system, we found that HAP1 also interacts wit

264 Using the two-hybrid system, we found that the extreme C-terminal

265 fragments in the bacterial adenylate cyclase two-hybrid system, we found that transketolase A (TktA)

266 Using the yeast two-hybrid system, we found that TRIM32 binds and ubiqui

267 Using the yeast two-hybrid system, we found that VZV IE63 interacts with

268 In the present study employing a yeast two-hybrid system, we found that zyxin, a molecule known

269 In this report, using the yeast two-hybrid system, we have identified a novel interactio

270 1 (Plk1)-interacting proteins using a yeast two-hybrid system, we have identified histone acetyltran

271 Using the yeast two-hybrid system, we have now identified two sperm-spec

272 Using the yeast two-hybrid system, we have observed a strong interaction

273 Using yeast two-hybrid system, we have previously identified C19ORF5

274 Using a modified yeast substrate trapping two-hybrid system, we identified a cytosolic adaptor pro

275 By using a yeast two-hybrid system, we identified a gene, invasion inhibi

276 Using the yeast two-hybrid system, we identified a number of proteins th

277 Using the yeast two-hybrid system, we identified a transcriptional coact

278 Using a yeast two-hybrid system, we identified Arabidopsis thaliana VI

279 Using the yeast two-hybrid system, we identified Hsp90, a chaperone with

280 Using the yeast two-hybrid system, we identified several second-site sup

281 Using the yeast two-hybrid system, we identified Sprouty2 as an interact

282 Using the yeast two-hybrid system, we identified uridine kinase like-1 (

283 Using the yeast two-hybrid system, we previously isolated a novel protei

284 By using the two-hybrid system, we recently identified Pin2/TRF1-inte

285 Using a yeast two-hybrid system, we screened for galectin-3-interactin

286 Exploiting the reverse two-hybrid system, we screened for mutated Imp4 proteins

287 Using chemical cross-linking and the yeast two-hybrid system, we show that sortilin interacts with

288 With the use of the yeast two-hybrid system, we show that this Dnm1p oligomerizati

289 Using a stringent, high-throughput yeast two-hybrid system, we tested pairwise interactions among

290 Using the yeast and mammalian two-hybrid systems, we observed that the CCR5 receptor i

291 changed to alanine could activate the yeast two-hybrid system when paired with RsbW, whereas mutant

292 sociated C terminus (BRCT) domain in a yeast two-hybrid system, while increased sensitivity of BRCT-d

293 , Pipkz1, was shown to interact in the yeast two-hybrid system with a putative bZIP transcription fac

294 r, interleukin-1, and ROS, we used the yeast two-hybrid system with ASK1 as bait to identify ASK1 sub

295 tor-1gamma (EF1gamma) interacts in the yeast two-hybrid system with DOA, the LAMMER protein kinase of

296 olin-1 was determined by the Pro-Quest yeast two-hybrid system with full-length NSP4 and seven overla

297 Combining the yeast two-hybrid system with genetic analysis, we show here th

298 The yeast two-hybrid system with myocilin as the bait and a human

299 identity with yeast Sec3p, interacts in the two-hybrid system with other subunits of the complex (Se

300 s for VHb on the cytochrome, using the yeast two-hybrid system with VHb as the bait and testing every