ライフサイエンスコーパス: two-photon

1 in what amounts to the sequential capture of two photons.

2 e we analyzed one-photon (1P) and degenerate two-photon (2P) absorption properties of several phospho

3 Two-photon (2p) glutamate uncaging at individual dendrit

4 sensitive, and bright voltage sensing using two-photon (2P) illumination.

5 Two-photon (2P) light-targeting strategies demonstrated

6 with application-oriented processes such as two-photon 3D patterning and data storage.

7 They were shown to be good two-photon absorbers, exhibiting high two-photon absorpt

8 Finally, attractive two-photon absorbing properties further underlined the g

9 at the telecom wavelength region due to the two photon absorption (TPA) and related effects.

10 l investigation is presented to estimate the two photon absorption (TPA) coefficient and the Kerr ref

11 l properties of the system, for both one and two photon absorption.

12 h power, and therefore cannot originate from two photon absorption.

13 heral substitution on triplet emissivity and two-photon absorption (2PA).

14 Interestingly, we observe an enhanced two-photon absorption (TPA) cross section for single Au2

15 resent a physical discussion of the indirect two-photon absorption (TPA) occuring in silicon carbide

16 ed dielectric metamaterial, considering both two-photon absorption and photo-induced free-carrier abs

17 onlinear light absorption mechanisms such as two-photon absorption and photon upconversion have been

18 symmetric quadrupolar molecules designed for two-photon absorption behave as dipolar molecules in the

19 e good two-photon absorbers, exhibiting high two-photon absorption coefficients.

20 rannulene and two thiophene units exhibits a two-photon absorption cross section of 600 GM and two-ph

21 tion of the pi-electrons, which enhances the two-photon absorption cross-section values of the antiar

22 iciency achieved through operating above the two-photon absorption edge, representing one of the larg

23 at the 1,550 nm wavelength resides above the two-photon absorption edge, while still possessing large

24 by the strong and coupled nonlinearities of two-photon absorption induced Drude electron-hole plasma

25 s of CPs and MOFs, with a closer look at the two-photon absorption property.

26 fects, linear and nonlinear losses including two-photon absorption, and photon-generated free carrier

27 ever, the impact of dimensional reduction on two-photon absorption, which is the Kramers-Kronig conju

28 e is subject to decoherence originating from two-photon absorption.

29 rs such as Stokes shift, emission color, and two-photon-absorption (2PA) cross section.

30 silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission

31 To this end, two-photon and one-photon absorption and emission spectr

32 a focus on multiply-scattered, fluorescence, two-photon, and phase imaging techniques to enhance GC c

33 To reduce fluorescence cross-talk for two photon applications, we present an innovative Two-Ph

34 nd the reach of traditional fluorescence and two-photon approaches and quantitation of their concentr

35 By expanding a two-photon beam in the imaging plane using the temporal

36 the transverse momentum correlation between two photons by detecting only one of them.

37 ational simulations with analysis of in vivo two-photon Ca(2+) imaging data from somatosensory cortex

38 Here we use in vivo whole-cell recording and two-photon Ca(2+) imaging in awake mice to show that lat

39 Using chronic two-photon Ca(2+) imaging in hippocampal area CA1 of wil

40 We address this question using two-photon Ca(2+) imaging in larval zebrafish with trans

41 Using two-photon Ca(2+) imaging in male rat acute brain slices

42 Here, we have used two-photon Ca(2+) imaging to monitor the activity of mos

43 Here, we used two-photon Ca(2+) imaging to study visual processing in

44 receptive field mapping, glutamate uncaging, two-photon Ca(2+) imaging, and genetic labeling of putat

45 Two-photon Ca(2+) imaging, triple immunofluorescent labe

46 immunocytochemistry, paired recordings, and two-photon Ca(2+) imaging, we analyzed excitatory synaps

47 Using two-photon calcium imaging and electrophysiological reco

48 Here, we use two-photon calcium imaging and electrophysiology in head

49 red the same effects on single neurons using two-photon calcium imaging and found that the increase i

50 Using two-photon calcium imaging and optogenetic manipulations

51 erebral ischaemia combined with fast in vivo two-photon calcium imaging and selective microglial mani

52 y first identifying integrator neurons using two-photon calcium imaging and then reconstructing the s

53 optical and computational methods, based on two-photon calcium imaging and two-photon optogenetics,

54 Simultaneous intracellular recording and two-photon calcium imaging confirm that fluorescence act

55 Neuroanatomical analysis and two-photon calcium imaging demonstrate that DALcl1 and D

56 We used two-photon calcium imaging in anesthetized and awake mic

57 Using two-photon calcium imaging in awake mice, we show that t

58 (OB), mitral and tufted cells, using chronic two-photon calcium imaging in awake mice.

59 Two-photon calcium imaging in awake mouse models showed

60 Here, using two-photon calcium imaging in behaving mice, we show tha

61 uron activity and movement, we used in vivo, two-photon calcium imaging in CA1 of male and female mic

62 We combined two-photon calcium imaging in head-fixed flying flies wi

63 Using in vivo two-photon calcium imaging in layers 2/3 and 4 in mouse

64 neuron activity and movement through in vivo two-photon calcium imaging in mice learning a lever-pres

65 We used two-photon calcium imaging in V1 of mice performing a st

66 Using in vivo two-photon calcium imaging of layer 2/3 barrel cortex ne

67 Two-photon calcium imaging of local cortical populations

68 Here we use in vivo two-photon calcium imaging of neocortical astrocytes whi

69 holographic method to simultaneously perform two-photon calcium imaging of neuronal populations acros

70 Two-photon calcium imaging of retino-recipient midbrain

71 Two-photon calcium imaging of secondary motor cortex (M2

72 Using two-photon calcium imaging on intact larval zebrafish, w

73 Two-photon calcium imaging provides an optical readout o

74 Furthermore, two-photon calcium imaging revealed that M2 ensemble act

75 Two-photon calcium imaging reveals that a thalamic nucle

76 In vivo two-photon calcium imaging reveals that these LC types r

77 provides an effective method for volumetric two-photon calcium imaging that increases the number of

78 To address this issue, we used in vivo two-photon calcium imaging to characterize the orientati

79 the origin of cortical maps, we used in vivo two-photon calcium imaging to characterize the propertie

80 we used intrinsic signal optical imaging and two-photon calcium imaging to map visual responses in ad

81 of Sapap3 KO mice was further explored using two-photon calcium imaging to monitor striatal output fr

82 Here we use in vivo two-photon calcium imaging to monitor the activity of do

83 Here, we have used two-photon calcium imaging to monitor the activity of yo

84 By combining two-photon calcium imaging to obtain dense retinal recor

85 Here we used two-photon calcium imaging to reveal an alternative arra

86 We then use two-photon calcium imaging to track individual cells chr

87 Finally, we use two-photon calcium imaging to track the matching process

88 Here, we used random-access two-photon calcium imaging together with electrophysiolo

89 ed this issue utilizing behavioral modeling, two-photon calcium imaging, and optogenetic inactivation

90 dLGN and V1, both with electrophysiology and two-photon calcium imaging, have described receptive fie

91 Finally, using two-photon calcium imaging, we show that SC direction se

92 Using in vivo two-photon calcium imaging, we studied how MCs responded

93 ex in vivo while measuring their output with two-photon calcium imaging.

94 ing learning over a week, using longitudinal two-photon calcium imaging.

95 Here we report a technique termed two-photon chemical apoptotic targeted ablation (2Phatal

96 use of photoinitiators, which makes it truly two-photon click chemistry.

97 Two-photon computer generated holography (2P-CGH) recent

98 Two-photon computer-generated holography enables precise

99 tively (epsilon440 = 66,000 M(-1) cm(-1) and two-photon cross section of 350 GM at 775 nm).

100 ncy devoid of side reactions and significant two-photon cross-section of NDBF render it superior to B

101 hoton applications, we present an innovative Two-Photon-Dye-Specific Excitation-Emission Offset (TP-D

102 We show that such two-photon emission processes can occur on nanosecond ti

103 ne-photon excitation approaches, their broad two-photon excitation (2PE) and poorly understood photob

104 ability to form triplet excited states upon two-photon excitation is important for several applicati

105 First second harmonic generation and two-photon excitation microscopy is used to map the micr

106 luorescence anisotropy imaging combined with two-photon excitation microscopy to map nanoscale diffus

107 By imaging calcium dynamics with two-photon excitation microscopy, we show that LC11 resp

108 ness and photostability under one-photon and two-photon excitation modes, respectively.

109 Then, we introduce a dynamic two-photon excitation protocol to simultaneously determi

110 Two-photon excitation provides high spatial resolution i

111 Irradiation with 365 nm light or two-photon excitation with 800 nm light resulted in effi

112 ndria and monitor mitochondrial Zn(2+) under two-photon excitation with low cytotoxicity.

113 m using second harmonic generation (SHG) and two photon excited autofluorescence.

114 We show multimodal superresolved images with two-photon excited fluorescence (TPEF) and second-harmon

115 Two-photon excited fluorescence (TPEF) imaging is a non-

116 for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tis

117 only used measurement techniques (Z-scan and two-photon excited fluorescence (TPEF)) that can be appl

118 Here, we demonstrate that intrinsic two-photon excited fluorescence enables functional imagi

119 ng coherent anti-Stokes Raman scattering and two-photon excited fluorescence microscopy, we show that

120 Raman scattering (SEHRS) is the spontaneous, two-photon excited Raman scattering that occurs for mole

121 SM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic gene

122 hoton absorption cross section of 600 GM and two-photon-excited intense green luminescence.

123 mized protocol for simultaneous dual-channel two-photon fluorescence anisotropy microscopy acquisitio

124 Two-photon fluorescence confocal microscopy and UV/Vis-a

125 sensors for monitoring kinase activity under two-photon fluorescence lifetime imaging microscopy (2pF

126 In this work, we employ two-photon fluorescence lifetime imaging microscopy (FLI

127 ce energy transfer-based sensor for TrkB and two-photon fluorescence lifetime imaging microscopy, we

128 CaMKII activity in spines using fast-framing two-photon fluorescence lifetime imaging.

129 t demonstration to integrate high-resolution two-photon fluorescence microscopy (TPM) with a 16.4 tes

130 ing functional reference atlases and in vivo two-photon fluorescence microscopy data from the same sp

131 We have also correlated CARS with two-photon fluorescence microscopy simultaneously acquir

132 pare it to a popular commercial system using two-photon fluorescence microscopy.

133 A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological

134 A two-photon fluorescent probe based on a ruthenium(II) vi

135 quency of the transition, which is driven by two photons from a laser at 243 nanometres, is consisten

136 To explore this, we used in vivo two-photon guided cell-attached recording in conjunction

137 By performing two-photon guided whole-cell recordings from layer 2/3 e

138 To answer these questions, we performed two-photon guided whole-cell Vm recordings from primary

139 In combination with two-photon holographic stimulation, we found that this s

140 zing a quantum resource engineered combining two-photon hyperentanglement and photonic-chip technolog

141 rs exhibit low sensitivity when imaged under two-photon illumination.

142 By providing two-photon imaging access to cortical neuronal populatio

143 Furthermore, biochemical and two-photon imaging analyses identified elevated and imba

144 Here, we used conventional and two-photon imaging and electron microscopy to show that

145 rodent whisker system using a combination of two-photon imaging and electrophysiology during active t

146 a novel combination of high-throughput live two-photon imaging and gene expression profiling to stud

147 n a mouse model of neuropathic pain, in vivo two-photon imaging and patch clamp recording showed init

148 Here we implemented combined two-photon imaging and photolysis in vivo to monitor and

149 bility to adaptively patch, under continuous two-photon imaging and real-time analysis, fluorophore-e

150 Here we report techniques for long-term two-photon imaging in awake macaque monkeys.

151 Here, by using two-photon imaging in awake macaques and systematically

152 Using in vivo two-photon imaging in female mice, we show that mPOA(Nts

153 rate that the combination of ASAP2s and fast two-photon imaging methods enables detection of neural e

154 We combined two-photon imaging microscopy in brain slices with in vi

155 onic mechanisms is quantitative and combined two-photon imaging of [Cl(-)]i and pHi, but this has nev

156 Using in vivo two-photon imaging of both awake and anesthetized mice,

157 signal imaging through the intact skull and two-photon imaging of calcium signals in single neurons.

158 Finally, using two-photon imaging of extracellular glutamate, we find t

159 shrew (Tupaia belangeri) visual cortex using two-photon imaging of GCaMP6 calcium signals.

160 By combining confocal and two-photon imaging of genetically encoded pH reporters w

161 We also show that ASAP2s enables two-photon imaging of graded potentials in organotypic s

162 Here we demonstrate chronic two-photon imaging of granule cell population activity i

163 neuron-pair operant conditioning task using two-photon imaging of IN subtypes expressing GCaMP6f.

164 n in a mouse model of acute lung injury with two-photon imaging of intact lung tissue.

165 Using two-photon imaging of large groups of neurons, we show t

166 ual cortex, we demonstrate that high-quality two-photon imaging of large neuronal populations can be

167 Two-photon imaging of migrating T cells in the steady-st

168 Dual-color in vivo two-photon imaging of mouse ACx showed pathway-specific

169 Here, we introduce volumetric two-photon imaging of neurons using stereoscopy (vTwINS)

170 We use in vivo, two-photon imaging of novel genetically encoded voltage

171 Two-photon imaging of redox-sensitive GFP corroborated t

172 Two-photon imaging of the genetically encoded fluorescen

173 Using intravital and kidney slice two-photon imaging of the three-dimensional structure of

174 O'Herron et al. (2016) perform two-photon imaging of vascular and neural responses in c

175 Concurrently, intravital two-photon imaging revealed prompt peritubular vasodilat

176 Two-photon imaging showed that many excitatory neurons i

177 However, conventional two-photon imaging systems are limited in their field of

178 In this study, we utilized in vivo two-photon imaging to directly monitor the acute structu

179 asive, high resolution, endogenous contrast, two-photon imaging to identify distinct adipose tissue t

180 Working within the delineated area, we used two-photon imaging to measure basic taste responses in >

181 Here, we used two-photon imaging to study the patterns of activity of

182 Here, we used in vivo two-photon imaging to track spines over multiple days in

183 Successful application of two-photon imaging with genetic tools in awake macaque m

184 Using in vivo two-color two-photon imaging with genetically encoded calcium indi

185 Under two-photon imaging, a single GAC generated rectified loc

186 ng chemogenetics and optogenetics, live cell two-photon imaging, cell fate reprogramming and human pl

187 visual cortex using a combination of in vivo two-photon imaging, morphological reconstruction, immuno

188 By combining computational simulation, two-photon imaging, optogenetics, and dual-color uncagin

189 Using live two-photon imaging, we demonstrate that the microglial r

190 Using paired recordings and two-photon imaging, we determined the properties of the

191 Using repeated in vivo two-photon imaging, we find that increases in spine size

192 haracterized by novel technologies including two-photon imaging, whole-genome transcriptomic and epig

193 l neurons to visual stimuli, as confirmed by two-photon imaging.

194 Two-photon in vivo imaging showed a weaker visual respon

195 find that the relationship between FRET and two-photon intensity quantitatively agrees with predicti

196 of Texas Red-labeled rat serum albumin using two-photon intravital microscopy.

197 adenine dinucleotide (NADH), was induced by two-photon laser excitation and its lifetime decay was a

198 A versatile two-photon laser lithography is employed for LC cell sca

199 cs of dural vessels during behavior, we used two-photon laser scanning microscopy (2PLSM) to measure

200 ges in axonal boutons imaged with time-lapse two-photon laser scanning microscopy (2PLSM).

201 Two-photon laser scanning microscopy of calcium dynamics

202 ine or chronic social defeat stress and used two-photon laser scanning microscopy with glutamate phot

203 dule that is easily integrated into standard two-photon laser-scanning microscopes to generate an axi

204 e made their implementation in studies using two-photon laser-scanning microscopy (TPLSM) challenging

205 Such speed is a challenge for conventional two-photon laser-scanning microscopy, because it depends

206 d for Au-containing composite fabrication by two-photon lithography.

207 l addition, which are then polymerized using two-photon lithography.

208 Using transcriptional studies and slow-scan two-photon live imaging capable of identifying the numbe

209 This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome

210 beta-imaging can be carried out in a regular two-photon microscope setup through a series of intensit

211 Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing

212 d anatomical tracers with a novel multi-area two-photon microscope to perform simultaneous calcium im

213 Here, we demonstrate a two-photon microscope with an expanded field of view (>9

214 cell(s) by focusing NIR light emitted from a two-photon microscope.

215 We developed an in vivo two-photon microscopic approach that allows performing d

216 c vesicles, from EC synaptic terminals using two photon microscopy in slices obtained from forebrain

217 adult V1 with chronic in vivo imaging using two-photon microscopy and determined the spine turnover

218 Here, we used the combination of intravital two-photon microscopy and frequency-domain fluorescence

219 r with advanced research techniques, such as two-photon microscopy and optical imaging.

220 To verify the results, two-photon microscopy combined with a microprism was als

221 In vivo ER imaging with two-photon microscopy detects similar ER enlargement, an

222 Time-lapse two-photon microscopy in adult slices was used to determ

223 Finally, using in vivo two-photon microscopy in anesthetized or awake-behaving

224 Using in vivo two-photon microscopy in mouse brain, we quantified mito

225 Here, using two-photon microscopy in vivo in mice, we demonstrate th

226 The advent of two-photon microscopy now reveals unprecedented, detaile

227 l area in awake and freely moving mice using two-photon microscopy or an nVista miniaturized microsco

228 Furthermore, the study of microglia by two-photon microscopy revealed that anti-ICAM-1/catalase

229 Two-photon microscopy revealed that exocytosis of GLP-1

230 of alpha-synuclein-GFP transgenic mice using two-photon microscopy showed that NPT100-18A reduced the

231 However, we observed by two-photon microscopy that mouse retinas incapable of ph

232 ericyte-labeled transgenic mice with in vivo two-photon microscopy to examine the relationship betwee

233 Here, we used acute and longitudinal in vivo two-photon microscopy to investigate developmental and e

234 Here we used two-photon microscopy to measure sensory-evoked response

235 We used two-photon microscopy to monitor ensemble activity durin

236 We have employed in vivo two-photon microscopy to produce time-lapse images of se

237 st this possibility physiologically, we used two-photon microscopy to record light stimulus-evoked Ca

238 One solution to this problem is to use two-photon microscopy to target fluorescently labeled ne

239 Here we show, using two-photon microscopy to track neural activity over mult

240 of a novel in vivo method we developed using two-photon microscopy to visualize the paravascular spac

241 keletal components and performing intravital two-photon microscopy to visualize TRM cell behavior.

242 culture cells and Xenopus egg extracts using two-photon microscopy with FLIM measurements of FRET.

243 cell-mediated model of EAE combining in vivo two-photon microscopy with two different activation repo

244 g with GFP-based probes in single-photon and two-photon microscopy, including time-lapse imaging in l

245 ch as wide-field and confocal one-photon and two-photon microscopy, to compare photochemical and biop

246 Applying intravital two-photon microscopy, we demonstrate the gatekeeper fun

247 Using transcranial two-photon microscopy, we examined the effect of visual

248 scent proteins, analysis of mutant mice, and two-photon microscopy, we follow long-range horizontally

249 three-color labeling and spectrally resolved two-photon microscopy, we monitor in parallel the daily

250 Using in vivo two-photon microscopy, we performed time-lapse imaging o

251 Using real-time in vivo two-photon microscopy, we quantified mitochondrial fragm

252 mained after HS treatment, as highlighted by two-photon microscopy.

253 rons in the mouse primary motor cortex using two-photon microscopy.

254 d autofluorescence emission with traditional two-photon microscopy.

255 6s-labeled neurons beyond the depth limit of two-photon microscopy.

256 order, employing, for example, confocal and two-photon microscopy.

257 ebrafish model of podocyte injury to in vivo two-photon microscopy.

258 not resolved by traditional intensity-based two-photon microscopy.

259 e in acute myeloid leukemia using intravital two-photon microscopy.

260 In two-photon mode, an increase in the field of view over t

261 t dictates reactivity of maleimide groups in two-photon mode.

262 Airy beams that operates both in single- and two-photon modes.

263 pecially as their design progresses to allow two-photon, near-infrared and radio-frequency activation

264 red-energy fluorescence can be stimulated by two-photon, near-infrared excitation so as to provide vi

265 Moreover, two-photon optogenetics enable the possibility of artifi

266 tent reconfiguration of cortical circuits by two-photon optogenetics into neuronal ensembles that can

267 re we report that repetitive activation with two-photon optogenetics of neuronal populations from ens

268 terneurons affect the local circuits, we use two-photon optogenetics to activate them individually in

269 ods, based on two-photon calcium imaging and two-photon optogenetics, to detect, characterize, and ma

270 ting on the polarization spatial DoFs of the two-photon or one-photon system.

271 applications of metalloporphyrins, including two-photon phosphorescence lifetime microscopy (2PLM) an

272 Two-photon photoconversion of cyanine-based dyes offer s

273 osphorescence lifetime microscopy (2PLM) and two-photon photodynamic therapy (PDT).

274 ate the case of Alq3 on Co and, by combining two-photon photoemission experiments with electronic str

275 given PUFA droplet in a spatially localized two-photon photosensitized process.

276 applied the recently developed technique of two-photon polarization microscopy (2PPM) to Galphai1 su

277 ing nanocomposite structures by simultaneous two-photon polymerisation and photoreduction is demonstr

278 ofessional GT 3D laser lithography system, a two-photon polymerization (2PP) 3D printer capable of mi

279 technique with scalability not achievable by two-photon polymerization or traditional stereolithograp

280 es (MWNTs) in 3D space is investigated via a two-photon polymerization technique.

281 laser writing (DLW) is a technique based on two-photon polymerization that allows the fabrication of

282 A naphthalimide-based two-photon probe (AHGa) for the detection of cell senesc

283 A two-photon process is employed to overcome a first order

284 t can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat

285 either reside within the highly dissipative two-photon regime in silicon-based optical devices, or p

286 r insignificant as demonstrated by broadband two-photon spectroscopy.

287 ate the generation of polarization-entangled two-photon state through spontaneous four-wave mixing in

288 uit function, which combines simultaneous 3D two-photon stimulation of multiple targeted neurons, vol

289 While studying two-photon structuring applications, we observed an unde

290 tatistics, distinguishing coherent, one- and two-photon superposed states with the finite (quantised)

291 Here we describe a platform for automated two-photon targeted patch-clamp recording, which solves

292 To address this issue, we made two-photon targeted patch-clamp recordings from rat TC a

293 lled and coherent exchange collision between two photons that is accompanied by a pi/2 phase shift.

294 Two-photon time-lapse imaging indicated that microglia d

295 laboratory has investigated this topic using two-photon time-lapse microscopy to directly visualize t

296 Using two-photon tomography, carrier lifetimes are mapped in p

297 otransmitter GABA in its natural state using two-photon uncaging microscopy for the first time.

298 r recordings from targeted spine heads under two-photon visualization.

299 presents medium-range atomic fluctuations at two photon wavelengths strongly suggest a density-driven

300 mes at which the simplest nonlinear process, two-photon X-ray absorption (TPA), can be observed.