ライフサイエンスコーパス: typing

1 e and virulence profiling, including capsule typing.

2 s, alongside genomic human leukocyte antigen typing.

3 statistically similar results for S. aureus typing.

4 susceptibility testing, and epidemiological typing.

5 nst adalimumab (AAA) levels and class II HLA typing.

6 , and isolates underwent multilocus sequence typing.

7 an alternative approach for molecular strain typing.

8 and appropriate dilution is suggested before typing.

9 SA isolates were characterized by spa typing.

10 sistance and virulence genes, SCCmec and spa typing.

11 cal CCs were assessed by multilocus sequence typing.

12 re cultured and isolates underwent molecular typing.

13 ifferences in Nei's genetic distance and SBI typing.

14 jor clusters as identified by microsatellite typing.

15 which are distinguishable only by molecular typing.

16 aboratory-based antimicrobial susceptibility typing.

17 ion method for phosphorylation stoichiometry typing.

18 them were individually validated through SNP typing.

19 agnosis and the need for unequivocal amyloid typing.

20 pproach for molecular epidemiological strain typing.

21 cal to 3 clinical isolates by sequence-based typing.

22 number tandem-repeat analysis (MLVA) and spa typing.

23 solates, which fell into 14 clonal groups on typing.

24 tive units-variable number of tandem repeats typing.

25 e set of markers for genetic Vel blood group typing.

26 d need to perform culture isolation prior to typing.

27 , available at referral centers, for amyloid typing.

28 quencing and core genome multilocus sequence typing (1546 loci) on serogroup W disease isolates from

29 ino acid level to achieve 99-100% four-digit typing accuracy for both class I and II HLA genes, takin

30 udy aimed to evaluate the semiautomated RFLP typing against the standard manual method.

31 rB allele 87, two of the multilocus sequence typing alleles that define ST648; and (ii) the typical a

32 However, microbial strain typing allows zoonotic pathogens to be categorised, and

33 ether tumor microsatellite instability (MSI) typing along with immunohistochemistry (IHC) and MLH1 me

34 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA

35 High-resolution MHC class II typing and a T-cell proliferation assay using preselecte

36 ic (gene comparisons and multilocus sequence typing and analysis), genomic (dDDH, ANI, and AAI) and p

37 cing is becoming a leading technology in the typing and epidemiology of microbial pathogens, but the

38 tinguish isolation sources was tested by SNP typing and GC assignment of 154 isolates from hospital p

39 Isolates underwent spa typing and hla sequencing.

40 gel electrophoresis and multilocus sequence typing and identified their blaESBL genes by sequencing.

41 Direct typing and imputation was used to fine-map the human leu

42 ventional techniques for A. baumannii strain typing and outbreak analysis.

43 ng (WGS) is a promising technique for strain typing and outbreak investigations.

44 Isolates underwent multilocus sequence typing and PCR sequencing of the wzi and blaKPC genes.

45 lates was assessed using multilocus sequence typing and phylogenetic analyses.

46 for global harmonization of Lm genome-based typing and population biology.

47 phylococcal cassette chromosome mec (SCCmec) typing and pulsed-field gel electrophoresis (PFGE) subty

48 nsors targeting loci associated with species typing and resistance to rifampin, isoniazid and fluoroq

49 Genome-wide SNP typing and Sanger sequencing revealed no pathogenic vari

50 Progress in typing and sequencing technologies might additionally co

51 Whole-genome multilocus sequence typing and single nucleotide polymorphism analyses provi

52 signatures, metagenomic multilocus sequence typing and strain-specific marker genes.

53 pecific single-nucleotide polymorphism (SNP) typing and targeted WGS to investigate a tuberculosis cl

54 lymerase chain reaction (PCR) for phylogroup typing and using PCR with primers for O25b rfb and allel

55 Molecular typing and virulence analysis are powerful tools that ca

56 ified and matched by high-resolution allelic typing and were compatible in at least 9 of 10 HLA loci.

57 1178 case-patients, 1082 (92%) with G and P typing, and 4927 controls.

58 ryngeal samples for viral load quantitation, typing, and genotyping, and blood samples for transcript

59 perform multilocus sequence typing, capsular typing, and identification of virulence and antimicrobia

60 taphylococcal protein A (spa) typing, SCCmec typing, and PCR-based assays were used to detect mecA, m

61 ssment of antiviral resistance, strain-level typing, and phylogenetic relatedness.

62 etermined by multilocus sequence typing, sap typing, and the presence/absence of insertion sequences

63 analysed for antibiotic resistance, plasmid typing, and transfer analysis, and strain relatedness.

64 chemical staining for p16INK4a and Ki67, HPV-typing, and viral load determination in AGWs of HIV+ com

65 el electrophoresis (PFGE) analysis, sequence typing, and virulence gene profiling.

66 Using serotyping, multilocus sequence typing, and whole-genome sequencing (WGS) of selected st

67 the 2-position and subsequent reaction with typing antiserum 35a.

68 network compared to current molecular strain typing approaches, including analysis of intra-host dive

69 ata enable the improvement of sequence-based typing approaches, such as multilocus sequence typing (M

70 that sequential live bacteria detection and typing are demonstrated on the same microfluidic platfor

71 Using ABO blood typing as a proof-of-concept, we developed i) an integra

72 ions for associating outbreaks with sequence typing as well as understanding mechanisms behind the gl

73 ning the allelic state of the HLA genes (HLA typing) as a by-product of standard whole-genome sequenc

74 that MLVA offers higher resolution than spa typing, as it resolved 17 versus 12 discrete isolate gro

75 igned a universal human papillomavirus (HPV) typing assay based on target enrichment and whole-genome

76 lly available HAdV PCR assay and a molecular typing assay for species identification.

77 Moreover, we developed and validated a gp60 typing assay for this species and applied it to 27 isola

78 he concept of presenting a paper-based blood typing assay in a barcode-like pattern significantly enh

79 Blood typing assay is a critical test to ensure the serologica

80 isolates, we developed a strain-specific SNP-typing assay to identify further cases.

81 ese channels were then used to perform blood typing assays by introducing a blood sample.

82 Typing assigned M. genitalium DLSTs to 2 major clusters,

83 l donor-recipient pairs had allele-level HLA typing at HLA-A, -B, -C, and -DRB1, which is the current

84 ommunity, and more robust and discriminatory typing at the single-nucleotide-polymorphism level.

85 d nanoparticle probes and 10(2)-10(4)CFU for typing bacteria by an on-chip polymerase chain reaction.

86 tform-independent program capable of rapidly typing bacterial isolates directly from raw sequence rea

87 ifferent bacterial strains, as achieved with typing based on genetic markers.

88 ous data set to create a multilocus sequence typing-based scheme that can identify Yersinia strains t

89 ne that dru typing is a useful tool for MRSP typing, being an objective, standardized, sequence-based

90 and provided oral-rinse samples for HPV DNA typing by polymerase chain reaction.

91 Molecular analyses included strain typing by pulsed-field gel electrophoresis, mec and acce

92 clude that guidelines for MALDI-TOF MS-based typing can be developed along the same lines as those us

93 cing was used to perform multilocus sequence typing, capsular typing, and identification of virulence

94 ncing with core genome multilocus sequencing typing (cgMLST) analysis for real-time surveillance and

95 to generate core genome multilocus sequence typing (cgMLST) data.

96 lopment of a core genome multilocus sequence typing (cgMLST) scheme for M. gallisepticum strains and

97 of conventional methods with WGS for strain typing clinical Acinetobacter isolates and analyzing a c

98 Sequence typing clusters the Rickettsia within the Torix group of

99 first application of eWGS for universal HPV typing could be a useful method to elucidate HPV epidemi

100 combined with multi-virulence-locus sequence typing (CRISPR-MVLST), and (iv) pulsed-field gel electro

101 ay was compared to the ELVIS HSV ID and D(3) Typing Culture System for the qualitative detection and

102 The discriminatory power for dru typing (D = 0.423) was comparable to that of spa typing

103 ng (D = 0.423) was comparable to that of spa typing (D = 0.445) and of MLST (D = 0.417) in the first

104 tion were characterized to compile molecular typing data spanning 37 years (1978-2014).

105 grants included in a secondary analysis with typing data were assumed index cases.

106 ariable-number tandem repeat analysis (MLVA) typing data, with only three discordant results observed

107 susceptibility testing, multilocus sequence typing, DiversiLab, and plasmid analyses.

108 en expression, creating a risk for Vel blood typing errors and transfusion reactions.

109 scent amplified fragment-length polymorphism typing established that no isolates were identical.

110 se genes are highly polymorphic, which makes typing expensive and time consuming.

111 to supplement consultation with local tissue typing experts.

112 atedness was assessed by multilocus sequence typing, fitness was evaluated by growth curve and compet

113 We obtained olfactory fingerprints and HLA typing for 130 individuals, and found that olfactory fin

114 The N. gonorrhoeae Sequence Typing for Antimicrobial Resistance (NG-STAR) molecular

115 Case records were reviewed and molecular typing for common carbapenemases was performed.

116 atient selection, use of high-resolution HLA typing for donor selection, and improved supportive care

117 lies on antigen-level (lower resolution) HLA typing for HLA-A and HLA-B, and allele-level for HLA-DRB

118 pert C. difficile/Epi to multilocus sequence typing for identification of C. difficile NAP1 and found

119 sive data on resistance mutations and strain typing for monitoring transmission, but unlike for conve

120 be more discriminatory than both MLVA and P1 typing for the M. pneumoniae isolates examined, providin

121 Donor Program/Be The Match has collected HLA typing from CLL patients in need of allogeneic hematopoi

122 HPV testing and typing from samples obtained for Papanicolaou testing oc

123 HPV typing has been recommended for colposcopy triage, but i

124 oning regimen, and with available allele HLA typing (HLA-A, -B, -C, and -DRB1).

125 Strain typing identified 23 different strains.

126 re highly prevalent, followed by manual RFLP typing if resolution is not achieved, thereby saving tim

127 ing applications, including bacterial strain typing, immunization of cultures, autoimmunity or self-t

128 study were to apply direct repeat unit (dru) typing in a large collection of well-characterized MRSP

129 Genome-wide single nucleotide polymorphism typing in two siblings with neonatal diabetes from a con

130 sessment (V) and it provides epidemiological typing information (T).

131 ng was used to determine multilocus sequence typing information and identify genes encoding antimicro

132 It can be installed in R by typing install.packages ('NAM').

133 The results of this study underline that dru typing is a useful tool for MRSP typing, being an object

134 is used as the substrate for epidemiological typing is both a virulence factor and a vaccine antigen.

135 the epidemiology of these infections, strain typing is required.

136 high-throughput, and technically undemanding typing method for epidemiological and phylogenetic inves

137 yping (MLST), is the current "gold standard" typing method for investigation of legionellosis outbrea

138 he potential to become the new gold standard typing method for L. pneumophila.

139 A short tandem repeat (STR) typing method is developed for forensic identification o

140 triction fragment length polymorphism (RFLP) typing method is highly discriminatory; however, it is l

141 = 0.87) and was found to be a more powerful typing method than MLVA and MLST combined (D = 0.67).

142 sified E. coli into four types using a novel typing method to monitor fimH mutation patterns of fecal

143 otyping marker obtained by a high-resolution typing method.

144 Standardised surveillance protocols and typing methodologies to monitor this emerging pathogen s

145 uster which would not be revealed by current typing methodologies.

146 Combining molecular typing methods and geographical information system (GIS)

147 While current blood typing methods are well established, results are subject

148 Molecular typing methods combined with spatial analysis suggest po

149 The typing methods included pulsed-field gel electrophoresis

150 Conventional bacterial typing methods lack the discriminatory power to confiden

151 Because current typing methods often fail to resolve the infection sourc

152 olates were highly concordant with the other typing methods performed.

153 y analyses and is more accurate than current typing methods used during outbreak investigations.

154 ollection, the discriminatory indices of the typing methods were calculated.

155 ype B and that the results of the two strain-typing methods would be comparable.

156 elation between the WGS data and our routine typing methods, although some discordant results were ob

157 ariable number tandem repeat assay and other typing methods.

158 enotyped using manual and semiautomated RFLP typing methods.

159 pean countries presented multilocus sequence typing (MLST) alleles, sequence types and emm types (onl

160 tion was performed using multilocus sequence typing (MLST) alongside traditional phenotypic methods.

161 Multi locus sequencing typing (MLST) analysis identified two strains of tsetse-

162 ensitivity inferred from multilocus sequence typing (MLST) and genome-wide SNP-based phylogenetic ass

163 d genes were analyzed by multilocus sequence typing (MLST) and sequence analysis of virulence-associa

164 these isolates included multilocus sequence typing (MLST) and staphylococcal protein A gene (spa) ty

165 using a newly developed multilocus sequence typing (MLST) approach and elucidates the diversity, dis

166 Although multilocus sequence typing (MLST) currently represents the gold standard for

167 ation structure based on multilocus sequence typing (MLST) data derived from the WGS data showed that

168 A multilocus sequence typing (MLST) database for V. parahaemolyticus was creat

169 mparison between WGS and multilocus sequence typing (MLST) identified major discrepancies for 17% of

170 Multilocus sequence typing (MLST) is the gold standard genotyping technique

171 A multilocus sequence typing (MLST) scheme for M. pneumoniae was developed bas

172 sity of this pathogen, a multilocus sequence typing (MLST) scheme was developed and applied to the ch

173 Multi locus sequence typing (MLST) suggests presence of genetic homogeneity a

174 to ST131 by the Achtman multilocus sequence typing (MLST) system and a screening PCR assay that targ

175 Nested multilocus sequence typing (MLST) was employed for case specimen extracts.

176 ctrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality.

177 ctrophoresis (PFGE), and multilocus sequence typing (MLST) were performed.

178 repeat analysis (MLVA), multilocus sequence typing (MLST), and pertactin gene (prn) mutational analy

179 The isolates underwent multilocus sequence typing (MLST), as well as assays for the Panton-Valentin

180 ping approaches, such as multilocus sequence typing (MLST), by substantially increasing the number of

181 ping (SBT), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing met

182 es confirmed as ST398 by multilocus sequence typing (MLST), which prompted retrospective analysis of

183 , largely congruent with multilocus sequence typing (MLST).

184 otypes assigned by using multilocus sequence typing (MLST).

185 olates were subjected to multilocus sequence typing (MLST).

186 d Egypt were subtyped by multilocus sequence typing (MLST).

187 amplicon sequencing for multilocus sequence typing (MLST).

188 oups defined by standard multilocus sequence typing (MLST).

189 014 was characterized by multilocus sequence typing (MLST).

190 RBCs, erythrocytes) means that careful blood-typing must be carried out prior to transfusion to avoid

191 Molecular typing of 246 Staphylococcus aureus isolates from unsele

192 We demonstrate the simple, multiplexed typing of a variety of epigenetic cancer biomarker 5mC w

193 Typing of amyloid fibrils, a critical determinant of the

194 n of live bacteria and another 40min for the typing of bacteria strains).

195 The accurate and reliable typing of blood groups is essential prior to blood trans

196 cular surveillance of noroviruses, including typing of both polymerase and capsid genes, is important

197 rdized protocol and centralized database for typing of C. difficile pathogens will increase comparabi

198 universally recognized, further careful sub-typing of cases may provide more precise genomic signals

199 he replacement of traditional serology-based typing of Escherichia coli by WGS is supported by user-f

200 Typing of group A Streptococcus (GAS) is crucial for inf

201 Accurate typing of HLA genes with short-read sequencing data has

202 view the different methods for detection and typing of HSV, including light microscopy, culture, sero

203 tion of this technology to the detection and typing of influenza and other viruses.

204 Typing of influenza virus strains is an important aspect

205 eful tool as a first-line method for routine typing of M. tuberculosis isolates, especially where Bei

206 Sequence typing of Metarhizium isolates revealed four species, wi

207 oratory equipment for the large-scale global typing of Mycobacterium avium subspecies paratuberculosi

208 Typing of Mycobacterium avium subspecies paratuberculosi

209 duced the IS6110-PvuII kit for semiautomated typing of Mycobacterium tuberculosis using the RiboPrint

210 Prospective typing of patients and donors in unrelated donor search

211 d independently validated a workflow for the typing of PTC clinical samples into CLIP2-positive and C

212 ed with conventional gel electrophoresis for typing of S. aureus isolates of bovine origin.

213 S-PCR) and virulence gene identification for typing of S. aureus strains.

214 Methods for haplotyping and DNA copy-number typing of single cells are paramount for studying genomi

215 Typing of strains revealed cocirculation of multiple mul

216 n addition, the molecular identification and typing of strains were also carried out.

217 HLA-C typing of target cells showed that KIR2DS2 recognition w

218 Typing of the isolates (n = 172) identified all 10 GBS s

219 nes from the microcosms as well phylogenetic typing of the isolates showed that ethenotrophs in the s

220 address this, we performed high-density SNP typing of the MHC in >32,000 individuals with IBD, impli

221 ologic examination is essential for accurate typing of the polyps to predict malignant potential and

222 Typing of these agents may complement disease assessment

223 -throughput alternative to direct laboratory typing of these loci and have enabled important findings

224 Continued molecular typing of this organism, particularly during outbreaks,

225 R assay represents a valuable tool for rapid typing of uncharacterized Shigella isolates and provides

226 sceptibility testing and multilocus sequence typing on Salmonella enterica serovar Typhimurium isolat

227 roup 1 isolates (n = 106) from the standard "typing panel," previously used by the European Society f

228 By 24 MIRU-VNTR typing, persons in clustered groups were 1.96 times more

229 bioinformatics whole-genome sequencing (WGS) typing pipelines at Public Health England are dependent

230 Urethral Nm isolates were similar by fine typing (PorA P1.5-1,10-8, PorB 2-2, FetA F3-6), except 2

231 igh cost, and limited availability of strain typing preclude their routine use.

232 The EQA participation helps to keep the typing procedures at a high standard and provides data f

233 Standard typing procedures use serological or amplification-based

234 was identified using a variety of molecular typing procedures, resulting in two P1 genotypes (types

235 Typing profiles were mainly patient specific.

236 tely and independently controlled a computer typing program 28 weeks after electrode placement, at th

237 Sequence-based typing provides multiple advantages over length-based me

238 mance exceeded previous iBCIs as measured by typing rate (by a factor of 1.4-4.2) and information thr

239 Anti-A or anti-B typing reagents were dried inside the microchannel of a

240 ture, allowing for comparison and sharing of typing results among laboratories worldwide.

241 equencing (NGS) has the potential to provide typing results and detect resistance genes in a single a

242 LST) and staphylococcal protein A gene (spa) typing results as well as SmaI macrorestriction patterns

243 ypic DST, Sanger sequencing, and traditional typing results for a collection of 91 strains.

244 Strain typing results were available for 2057 cases.

245 Typing results were compared to those of amplified fragm

246 HLA typing revealed that 155 relatives carried this protecti

247 Strain typing revealed that the cluster was comprised of hetero

248 bacteriophage and their insertion sites (SBI typing) revealed that cattle and sheep isolates had stat

249 pulsed-field gel electrophoresis (PFGE) for typing S. aureus Forty-two S. aureus isolates from three

250 which were determined by multilocus sequence typing, sap typing, and the presence/absence of insertio

251 Sequence-based typing (SBT), analogous to multilocus sequence typing (M

252 lm formation, Staphylococcal protein A (spa) typing, SCCmec typing, and PCR-based assays were used to

253 e backward compatibility with currently used typing schemas to facilitate comparisons and understandi

254 e have created the first multilocus sequence typing scheme (MLST) for P. larvae, which largely confir

255 say is the most phylogenetically informative typing scheme available for Y. enterocolitica.

256 The new typing scheme facilitates epidemiological study of this

257 The absence of a repeatable, universal typing scheme for the causative bacterium Paenibacillus

258 BT and can be used as part of a hierarchical typing scheme that should maintain backwards compatibili

259 Antimicrobial Resistance (NG-STAR) molecular typing scheme uses the DNA sequences of 7 genes (penA, m

260 (ERIC)-polymerase chain reaction (PCR)-based typing scheme's divisions while providing added resoluti

261 terns and the same MLST (multilocus sequence typing) sequence type (ST-1068) regardless of their geog

262 A commercial sequence-based typing service for C. glabratathat targets polymorphic t

263 Typing studies suggest that most cases of hospital-onset

264 10(-6)/bp/generation, consistent with sperm-typing studies, and infer that tract lengths span at lea

265 The Meningococcal Antigen Typing System (MATS) was used to estimate coverage by 4C

266 se that the scheme is an important molecular typing system to allow accurate and reproducible identif

267 This paper presents a microfluidic blood typing system using a small quantity of blood sample to

268 ); however, conventional multilocus sequence typing (targeting 6 conserved loci) and whole-genome seq

269 The most popular C. difficile-typing technique is PCR ribotyping, and we previously de

270 We performed two band-based typing techniques (pulsed-field gel electrophoresis and

271 old when WGS was used instead of traditional typing techniques.

272 ere collected and characterized by molecular typing techniques.

273 aper-based analytical device (PAD) for blood typing that allows for the simultaneous determination of

274 l-characterized MRSP isolates and to use dru typing to analyze a collection of previously uncharacter

275 We undertook tumor typing to classify MMR defects to determine if MMR statu

276 Genetic typing, to establish whether trends of antimicrobial res

277 A curated Web-based user-friendly sequence typing tool based on antimicrobial resistance determinan

278 WGS has come of age as a molecular typing tool to inform national surveillance of STEC O157

279 To assist in viral discovery, PaVE offers a typing tool; a simplified algorithm to determine whether

280 There is a need for accurate and low-cost typing tools that produce comparable data across studies

281 uzi species is categorized into six discrete typing units (TcI to TcVI) of which TcI is most abundant

282 lementation of human leukocyte antigen (HLA) typing using Polysolver or HLAminer combined with custom

283 tional platform for quantitative blood group typing via SPR detection is achieved by immobilizing ant

284 Occasionally, dru typing was able to further discriminate between isolates

285 nce of semiautomated RFLP compared to manual typing was excellent, with high discriminatory index (0.

286 HLA typing was performed for 449 participants.

287 HLA typing was performed in 4 patients, all of whom carried

288 Molecular typing was performed in almost all studies, with pulse f

289 MHC-I typing was performed on cellular RNA using Roche/454 pyr

290 Multilocus sequence typing was successfully completed on 494 isolates of Str

291 B (n=13), AB (n=9), O (n=14), and Rh (n=48) typing were 92%, 85%, 89%, 93%, and 96%, respectively, i

292 Methicillin resistance testing and spa typing were performed for all S. aureus isolates.

293 Endomysial antibodies and HLA typing were routinely available in only half of the cent

294 whole-genome single-nucleotide-polymorphism typing (WGST), (ii) multiple-locus variable-number tande

295 This minireview explores MALDI-TOF MS-based typing, which has already been performed on many clinica

296 d identify 43 genetically diverse progeny by typing with microsatellites and 9230 single-nucleotide p

297 HLA typing with NGS should prove useful to select participan

298 is presented for live bacteria detection and typing within a short period of time (30min for the dete

299 and pyrosequencing under the term 'NGS+' for typing Y-STRs and Y-chromosomal single nucleotide polymo

300 Our typing yielded 84.4% (411/487) concordance with traditio