ライフサイエンスコーパス: ubiquitin chain

1 in upon ubiquitin transfer to a substrate or ubiquitin chain.

2 adation by the 26S proteasome by attaching a ubiquitin chain.

3 hort-lived proteins that are labeled with an ubiquitin chain.

4 d that AMSH1 specifically cleaves K63-linked ubiquitin chains.

5 trameric, and longer Lys48- and Lys63-linked ubiquitin chains.

6 uitin chains but does not bind Lys-48-linked ubiquitin chains.

7 enzyme that removes Lys63- or linear-linked ubiquitin chains.

8 n 21 (TRIM21) and are positive for lysine-48 ubiquitin chains.

9 ppaB activation through its interaction with ubiquitin chains.

10 the E3 ligase HOIP, which synthesizes linear ubiquitin chains.

11 cleaves ubiquitinated proteins and releases ubiquitin chains.

12 In vitro, only JosD2 can cleave ubiquitin chains.

13 dification specifically by lysine(63)-linked ubiquitin chains.

14 low affinity receptor for long lysine-linked ubiquitin chains.

15 , controls NF-kappaB signaling by binding to ubiquitin chains.

16 ge complex consisting of four RIG-I and four ubiquitin chains.

17 h cells expressing NEMO that binds to linear ubiquitin chains.

18 domain of NEMO and linear (Met-1-linked) di-ubiquitin chains.

19 in intraneuronal inclusions often containing ubiquitin chains.

20 n (residues 120-133) and attaches K63-linked ubiquitin chains.

21 preference for proteins that carry multiple ubiquitin chains.

22 the U4 snRNP, with nonproteolytic K63-linked ubiquitin chains.

23 monoubiquitin, it can also be modified with ubiquitin chains.

24 DAT is mainly conjugated with Lys(63)-linked ubiquitin chains.

25 ghly adaptive and cooperative toward binding ubiquitin chains.

26 of the canonical proteolytic signal, Lys-48 ubiquitin chains.

27 lysine 48 (K48)- and lysine 63 (K63)-linked ubiquitin chains.

28 N-terminal alpha-amino group to build linear ubiquitin chains.

29 n types of homotypic chains as well as mixed ubiquitin chains.

30 quitin ligase (Ufd4p), resulting in branched ubiquitin chains.

31 ivated Uch37 cannot disassemble hRpn13-bound ubiquitin chains.

32 targets Ku80 that is modified by K48-linked ubiquitin chains.

33 inding activates CYLD-mediated hydrolysis of ubiquitin chains.

34 age toward the distal isopeptide bond in tri-ubiquitin chains.

35 ed to the formation of an array of polymeric ubiquitin chains.

36 protein degradation-associated Lys-48-linked ubiquitin-chains.

37 We show that K63-linked ubiquitin chains accumulate on tankyrase 1 in late S/G2

38 , in the presence of STAM, the length of the ubiquitin chains affects the apparent cleavage rate.

39 y an E2 enzyme, UBE2S/E2-EPF, that elongates ubiquitin chains after the substrates are pre-ubiquitina

40 K63-branched ubiquitin chains also regulate IL-1-inducible phosphoryl

41 monoubiquitin and Lys-48- and Lys-63-linked ubiquitin chains, although in differing proportions.

42 tion of ubiquitin aldehyde, which mimics the ubiquitin chain and binds to 26 S-associated deubiquitin

43 n monomers or attachment of a preformed poly-ubiquitin chain and requiring either a single pair of ub

44 tions as a high affinity receptor for linear ubiquitin chains and a low affinity receptor for long ly

45 ld complex sequentially cleaved Lys63-linked ubiquitin chains and catalyzed Lys48-linked ubiquitinati

46 he conformational ensembles of the above tri-ubiquitin chains and chains possessing the same linkages

47 to show how the complex binds Lys(63)-linked ubiquitin chains and cleaves at the distal end.

48 s an E3 ligase complex that generates linear ubiquitin chains and is important for tumour necrosis fa

49 in Lewy bodies is largely due to K63-linked ubiquitin chains and markedly reduced in the substantia

50 nd deubiquitination have employed unanchored ubiquitin chains and mono-ubiquitinated proteins.

51 nd substrates for modification with branched ubiquitin chains and points to an important role of thes

52 less is understood about the other types of ubiquitin chains and proteasome-independent functions.

53 ex), which catalyzes the formation of linear ubiquitin chains and regulates immune and apoptopic sign

54 atalyzed the formation of Lys63 (K63)-linked ubiquitin chains and stimulated the transcription factor

55 quitin homeostasis with accumulation of free ubiquitin chains and ubiquitinated substrates in the leo

56 ative conjugation signals (monoubiquitin and ubiquitin chains) and interactions with ubiquitin-bindin

57 onoubiquitin and Lys(48)- and Lys(63)-linked ubiquitin chains), and that wild-type and mutant RNF170

58 Importantly, although Lys-48-linked ubiquitin chains appear to trigger proteasomal degradati

59 K48-linked ubiquitin chains are added almost exclusively to BIR2-bo

60 A variety of ubiquitin chains are attached as selective labels on pro

61 Ubiquitin chains are bound along the outer rim of the he

62 d to proteasomes, proteins conjugated to K63-ubiquitin chains are directed to lysosomes.

63 53BP1, RAP80 and ubiquitin chains are enlarged following POH1 depletion b

64 with OPTN and the ability of OPTN to bind to ubiquitin chains are essential for TBK1 recruitment and

65 Ubiquitin chains are formed as structurally distinct pol

66 The pathway by which ubiquitin chains are generated on substrate through a ca

67 ably, the attached Lys-48- and Lys-63-linked ubiquitin chains are homogeneous and are segregated to s

68 Linear ubiquitin chains are important regulators of cellular si

69 a cells determines that the levels of Lys-63 ubiquitin chains are insensitive to short-time proteasom

70 Thus, unanchored ubiquitin chains are key signaling molecules that connec

71 Branched ubiquitin chains are particularly challenging, as multip

72 hains to tankyrase 1, while in G1 phase such ubiquitin chains are removed by BRISC, an ABRO1/BRCC36-c

73 Thus, even though ubiquitin chains are removed early in degradation, durin

74 1 is therefore important to ensure that poly-ubiquitin chains are removed only from committed substra

75 Finally, ubiquitin chains are trimmed by the deubiquitinating enz

76 ults underscore the importance of K11-linked ubiquitin chains as critical regulators of mitotic prote

77 nzyme (DUB) that hydrolyzes lysine-63-linked ubiquitin chains as part of distinct macromolecular comp

78 tain Met4 in a dormant state by a regulatory ubiquitin chain assembled by SCF(Met30).

79 Recently linear ubiquitin chains assembled by a complex containing HOIL-

80 inked multi-monoubiquitination on K29-linked ubiquitin chains assembled by the ubiquitin ligase (Ufd4

81 The ubiquitin chains assembled on Pex29 in vivo by Ufd2 main

82 Importantly, proper ubiquitin chain assembly by PJA2 requires that Tat first

83 modulate the interaction between the linear ubiquitin chain assembly complex (LUBAC) and the deubiqu

84 1, MALT1, and the HOIP subunit of the linear ubiquitin chain assembly complex (LUBAC) but not the HOI

85 The E3 ligase linear ubiquitin chain assembly complex (LUBAC) facilitates NEM

86 w a catalytic-independent role of the linear ubiquitin chain assembly complex (LUBAC) in lymphocyte a

87 The linear ubiquitin chain assembly complex (LUBAC) is a multimeric

88 Linear Ubiquitin chain Assembly Complex (LUBAC) is an E3 ligase

89 linked polyubiquitin (Met1-Ub) by the linear ubiquitin chain assembly complex (LUBAC) is an important

90 The linear ubiquitin chain assembly complex (LUBAC) is essential fo

91 The linear ubiquitin chain assembly complex (LUBAC) is the only kno

92 The linear ubiquitin chain assembly complex (LUBAC) regulates immun

93 The linear ubiquitin chain assembly complex (LUBAC), consisting of

94 PIN (Sharpin(cpdm) mice), a member of linear ubiquitin chain assembly complex (LUBAC), develop severe

95 RNF31, a component of the linear ubiquitin chain assembly complex (LUBAC), regulates cell

96 These chains are generated by the linear ubiquitin chain assembly complex (LUBAC), the only known

97 ng HOIL-1 and HOIP, components of the linear ubiquitin chain assembly complex (LUBAC), which has a pi

98 We found that the linear ubiquitin chain assembly complex (LUBAC), which was prev

99 deficient in K63 ubiquitin chains or linear ubiquitin chain assembly complex (LUBAC)-mediated linear

100 ng HOIL-1 and HOIP, components of the linear ubiquitin chain assembly complex (LUBAC).

101 in ligase HOIL-1L, a component of the linear ubiquitin chain assembly complex (LUBAC).

102 urther reveal that cFLIPLrequires the linear ubiquitin chain assembly complex and the kinase TAK1 for

103 We identify HOIL1 of the linear ubiquitin chain assembly complex as a novel MALT1 substr

104 The linear ubiquitin chain assembly complex serves as a previously

105 is the catalytic component of LUBAC (linear ubiquitin chain assembly complex), a multisubunit E3 lig

106 biquitin ligase complex called LUBAC (linear ubiquitin chain assembly complex), which catalyzes the f

107 entral catalytic factor of the LUBAC (linear ubiquitin chain assembly complex).

108 s showed that HOIL-1, a member of the linear ubiquitin chain assembly complex, contributes to activat

109 The linear ubiquitin chain assembly complex, LUBAC, is the only kno

110 nder native conditions to date is the linear ubiquitin chain assembly complex, of which the catalytic

111 y SHARPIN as a third component of the linear ubiquitin chain assembly complex, recruited to the CD40

112 protein Sharpin is a component of the linear ubiquitin chain assembly complex, which regulates NF-kap

113 with HOIP and HOIL-1, constitute the linear ubiquitin chain assembly complex.

114 ns), E3 (ubiquitin-protein ligases), and E4 (ubiquitin chain assembly factors).

115 ntaining ubiquitylation enzyme that promotes ubiquitin chain assembly on substrates.

116 inus of the proximal Ub, which allows, after ubiquitin chain assembly, the introduction of various re

117 ons point out that two parameters accelerate ubiquitin chain assembly: the increasing number of CUE b

118 g protein), a component of the LUBAC (linear ubiquitin chain-assembly complex), regulates inflammatio

119 SHARPIN forms a linear-ubiquitin-chain-assembly complex that promotes signaling

120 Ubiquitin chains assessed using alpha-FK2 antibodies are

121 present the crystal structure of a mixed tri-ubiquitin chain at 3.1-A resolution.

122 36 regulates the abundance of Lys(63)-linked ubiquitin chains at chromatin and that one of its substr

123 oncentrations and for an in vitro-formulated ubiquitin chain attached to a substrate protein.

124 nstrate the facile conjugation to K48-linked ubiquitin chains, bearing up to four ubiquitins, through

125 NleE inactivates the ubiquitin chain binding activity of host proteins TAK1-b

126 nt phosphorylation on S473 and S513 promotes ubiquitin chain binding in vitro as well as TBK1 activat

127 HeLa cells requires OPTN and NDP52 and OPTN ubiquitin chain binding.

128 tif and can disassemble Rad18-dependent poly-ubiquitin chains both in vitro and in vivo.

129 As translocation proceeds, ubiquitin chains bound to substrate are drawn to the cha

130 at spartin, via the UBR, binds Lys-63-linked ubiquitin chains but does not bind Lys-48-linked ubiquit

131 d that its C-terminal UBA domain can bind to ubiquitin chains but that the Dsc2 UBA domain is not ess

132 bute equally to the high-affinity binding of ubiquitin chains, but in their absence, ubiquitin conjug

133 separate IP(3)R subunits, and Lys-48-linked ubiquitin chains, but not Lys-63-linked chains, are requ

134 y targeting protein-protein interactions, or ubiquitin chains, but the details of the inhibition mech

135 me E1 by PYR-41 or blocking the formation of ubiquitin chains by over-expressing the lysine to argini

136 The ubiquitination of NEMO with linear ubiquitin chains by the E3-ligase LUBAC is important for

137 ent of U4 snRNP, is modified with K63-linked ubiquitin chains by the PRP19 complex and deubiquitinate

138 ignal than shorter chains, and (3) the tetra-ubiquitin chain can be degraded with the substrate.

139 The processive formation of polymeric ubiquitin chains can accordingly be catalyzed by special

140 uitin to substrates modified with K29-linked ubiquitin chains, can the substrates be escorted to the

141 nd that, although the kcat of Lys(63)-linked ubiquitin chain cleavage was comparable for di- and tri-

142 Ubiquitin chain conformations in isolation are often dif

143 et al. describe how viral RNA and unanchored ubiquitin chains conspire to promote activation of RIG-I

144 main of STAM and required that the substrate ubiquitin chain contain homogenous Lys(63)-linkages.

145 We investigated this idea by engineering di-ubiquitin chains containing differential proximal and di

146 on is a posttranslational modification where ubiquitin chains containing isopeptide bonds linking one

147 Posttranslational modification with ubiquitin chains controls cell fate in all eukaryotes.

148 cificities of these proteins for K48- or K63-ubiquitin chains determine whether a ubiquitinated prote

149 BRAXAS allows establishment of the 53BP1 and ubiquitin chain-devoid core.

150 Poly-ubiquitin chains direct protein substrates to the 26S pr

151 With its ability to bind ubiquitin chains directly and the proteasome via differe

152 In eukaryotes, the covalent attachment of ubiquitin chains directs substrates to the proteasome fo

153 ond UBL-binding site ( T2: ) that assists in ubiquitin chain disassembly, by binding the UBL of deubi

154 In contrast, linear, noncleavable ubiquitin chains do not promote DNA damage tolerance, bu

155 e IKK complex that occurs when NEMO binds to ubiquitin chains during pathway activation.

156 Our results indicate that ubiquitin chain editing is key to the cytosolic protein

157 he generation of K29 chains in vitro using a ubiquitin chain-editing complex consisting of the HECT E

158 r, APC engages and activates its specialized ubiquitin chain-elongating E2 UBE2S in ways that differ

159 trates without impairing the Ube2S-dependent ubiquitin chain elongation activity.

160 initiation of the ubiquitination process and ubiquitin chain elongation after deneddylation.

161 ng of the UEV domain to Rim8 interferes with ubiquitin chain elongation and directs Rim8 monoubiquiti

162 E4 enzymes that are specifically involved in ubiquitin chain elongation but whose roles in proteolysi

163 Ubiquitination of a subset of proteins by ubiquitin chain elongation factors (E4), represented by

164 by multiple LUBAC components, whereas linear ubiquitin chain elongation is realized by a specific int

165 allows Ube2S to bind the APC/C and catalyze ubiquitin chain elongation.

166 ding distinctive RING E3 features specifying ubiquitin chain elongation.

167 he canonical function of Poh1, which removes ubiquitin chains en bloc from proteasomal substrates pri

168 In this study, we employ Ubiquitin Chain Enrichment Middle-down Mass Spectrometry

169 ically, beta-catenin modified with lysine-11 ubiquitin chain extension efficiently activates a lympho

170 NCL ubiquitinates beta-catenin with atypical ubiquitin chain extension known to have nonproteolytic f

171 The usage of ubiquitin chains for the proper assembly and function of

172 97 substrate-Ub-GFP modified with K48-linked ubiquitin chains-for in vitro p97 activity assays.

173 The mechanism of ubiquitin chain formation by APC and the resultant chain

174 We now show that also Lys(63)-linked ubiquitin chain formation is required for GHR endocytosi

175 Our data also confirm that Vif could induce ubiquitin chain formation on lysine residues intersperse

176 t compounds as inhibitors of the proteasome, ubiquitin chain formation or perception, CRL activity, o

177 that monomeric E2s promote linkage-specific ubiquitin chain formation through substrate-assisted cat

178 stabilises RACO-1 by facilitating K63-linked ubiquitin chain formation, and enables RACO-1 dimerisati

179 t1 (CBM) complex and removes the TCR-induced ubiquitin chain from Bcl10, which facilitates the associ

180 ly of enzymes cleaves mono-ubiquitin or poly-ubiquitin chains from a target protein through different

181 ysis provides evidence that CYLD removes K48 ubiquitin chains from p53 indirectly by cleaving K63 lin

182 eferentially removes non-canonical K6-linked ubiquitin chains from parkin, a process required for the

183 Removal of ubiquitin chains from targeted substrates at the proteas

184 USP38 specifically cleaves K33-linked poly-ubiquitin chains from TBK1 at Lys670, and it allows for

185 (DUBA), which selectively cleaves K63-linked ubiquitin chains from TRAF3, was up-regulated in the abs

186 inating enzyme, USP29 binds to, cleaves poly-ubiquitin chains from, and stabilizes p53.

187 These ubiquitin chains function as an endogenous ligand of RIG

188 Understanding the precise roles of ubiquitin chains, however, is difficult due to their com

189 e USP2 enzyme, which was found to cleave the ubiquitin chain in a similar manner to unanchored ones.

190 mplex preferentially deubiquitinating the M1 ubiquitin chain in vitro.

191 l-length NEMO binds preferentially to linear ubiquitin chains in competition with lysine-linked ubiqu

192 specifically generate histone Lys-63-linked ubiquitin chains in DSB signaling.

193 Quantitative mass spectrometric analysis of ubiquitin chains in HeLa cells determines that the level

194 The role of Lys-63 ubiquitin chains in targeting proteins for proteasomal d

195 but interfacing barriers to promote loss of ubiquitin chains in the IRIF core, which is required for

196 c-finger domain (UBZ), and binds K-63-linked ubiquitin chains in vitro.

197 inhibits its ability to serve as a donor for ubiquitin chain initiation or elongation, but can be res

198 have been reported for the assembly of poly-ubiquitin chains, involving either stepwise transfer of

199 AK1 to TNFR1, suggesting that the K63-linked ubiquitin chain is not capable of recruiting IKK in vivo

200 Protein modification with ubiquitin chains is an essential signaling event catalyz

201 Removal of ubiquitin chains is controlled by ABRO1/BRCC36 and occur

202 l modification of cell-cycle regulators with ubiquitin chains is essential for eukaryotic cell divisi

203 known about how the progressive assembly of ubiquitin chains is managed by the responsible enzymes.

204 Removal of ubiquitin chains is mediated by deubiquitinases (DUBs).

205 The assembly of most ubiquitin chains is promoted by E2s, yet how these enzym

206 The function of linear ubiquitin chains is regulated at multiple levels: genera

207 nd non-covalent interactions with K63-linked ubiquitin chains (K63-Ubn) were shown to occur in its si

208 rticular route of degradation likely include ubiquitin chain length and linkage type, which may favor

209 findings reveal a novel mechanism to control ubiquitin chain length on substrates in vivo.

210 y)ubiquitin and found that, depending on the ubiquitin chain length, the orientation of the resulting

211 ing in the recruitment of IKK and the linear ubiquitin chain ligase LUBAC, which is essential for IKK

212 We demonstrate that this ubiquitin chain linkage switching reaction is essential

213 qualitative method that yields insights into ubiquitin chain linkage types and architecture within ho

214 ts suggest that Ufd2p functions by switching ubiquitin chain linkages to allow the degradation of pro

215 review recent studies of ubiquitin ligases, ubiquitin chain linkages, and ubiquitin binding proteins

216 RNAPII, an Elc1/Cul3 complex then produces a ubiquitin chain linked via lysine 48, which can trigger

217 nserved proteasome subunit Dss1 (Sem1) binds ubiquitin chains linked by K63 and K48.

218 evel, it is crucial to have facile access to ubiquitin chains linked to protein substrates.

219 ative ubiquitin chains such as linear or K11 ubiquitin chains may also play a role in certain pathway

220 ineered to bind exclusively to Lys-63-linked ubiquitin chains mediated partial NF-kappaB activation c

221 e Keap1 was polyubiquitinated with lysine-63-ubiquitin chains, modifications known to increase their

222 This study suggests that USP14 removes the ubiquitin chain of I-kappaB, therefore inducing I-kappaB

223 abilizes Beclin-1 by removing the K11-linked ubiquitin chains of Beclin-1 at lysine 437.

224 bilization by recruiting USP14 to cleave the ubiquitin chains of cGAS at lysine (K) 414.

225 tin chains in competition with lysine-linked ubiquitin chains of defined length, including long Lys-6

226 Ubiquitin chains of different topologies trigger distinc

227 oreover, OTUD1 cleaves Lysine 33-linked poly-ubiquitin chains of SMAD7 Lysine 220, which exposes the

228 act with Lys-11-, Lys-48-, and Lys-63-linked ubiquitin chains of varying length in cells.

229 The length of the ubiquitin chain on a substrate dictates various function

230 at inhibition is mediated by trimming of the ubiquitin chain on the substrate.

231 strongly suggest that removal of K63-linked ubiquitin chains on alpha-synuclein by Usp8 is a critica

232 Ls activate the RBR enzyme ARIH1 to initiate ubiquitin chains on CRL substrates, thereby marking an u

233 ause its recruitment signal, K63-linked poly-ubiquitin chains on histones, is actively destroyed by t

234 with the E2 enzyme UbcH5b in order to ligate ubiquitin chains on its substrates.

235 through noncovalent interactions between the ubiquitin chains on Mdm2 and the ubiquitin binding domai

236 adaptor phosphorylation with the assembly of ubiquitin chains on mitochondria to facilitate efficient

237 We report that assembly of ubiquitin chains on mitochondria triggers autophagy adap

238 te the ubiquitin ligase parkin, which builds ubiquitin chains on mitochondrial outer membrane protein

239 eflect the assembly of structurally distinct ubiquitin chains on target proteins.

240 In contrast, JosD1 cleaves ubiquitin chains only after it is monoubiquitinated, a f

241 rged with the essential task of synthesizing ubiquitin chains onto protein substrates.

242 ese structures, using cells deficient in K63 ubiquitin chains or linear ubiquitin chain assembly comp

243 ytic ubiquitin signaling mediated by Lys(63) ubiquitin chains plays a critical role in multiple pathw

244 be activated by such proteins even without a ubiquitin chain present.

245 Mechanistically, linear ubiquitin chains preserve the architecture of the TNFR1

246 We present evidence that free ubiquitin chains produced by Poh1 bind and activate the

247 resses 26S proteasome remodeling, unanchored ubiquitin chain production, and aggresome clearance.

248 Hence, conformational equilibria in ubiquitin chains provide an additional layer of regulati

249 Methionine-1 (M1)-linked ubiquitin chains regulate the activity of NF-kappaB, imm

250 he depolymerization kinetics of Lys48-linked ubiquitin chains relative to Lys63-linked chains.

251 d with the ATPase motor to prevent premature ubiquitin chain removal and substrate escape.

252 of the proteasome can be regulated by rapid ubiquitin chain removal, which resolves substrates based

253 Ubiquitin chains represent a biologically important mult

254 Ubiquitin chains represent another example of nature's a

255 Specifically, PARKIN-synthesized ubiquitin chains represent targets for the PINK1 kinase

256 the dynamic appendage of different types of ubiquitin chains represents a versatile, three-dimension

257 microinjection of free lysine (K) 63-linked ubiquitin chains restores aggresome degradation.

258 Ubiquitin chain-restriction analysis provides evidence t

259 We also show that, in the presence of K63 ubiquitin chains, RIG-I catalyzes the conversion of MAVS

260 and they explain the determinants of linear ubiquitin chain specificity by LUBAC.

261 cent research suggests that some alternative ubiquitin chains such as linear or K11 ubiquitin chains

262 host-derived glycans and K48- and K63-linked ubiquitin chains, suffices to restrict bacterial prolife

263 ential modifier efficiently binds K11-linked ubiquitin chains, suggesting that this ubiquitin linkage

264 l degradation, the presence of Lys-63-linked ubiquitin chains suggests that ubiquitination of IP(3)Rs

265 Proteins to be degraded are conjugated to ubiquitin chains that act as recognition signals for the

266 degradation by the 26 S proteasome using the ubiquitin chains that mark most substrates for degradati

267 partners, in addition to removing K63-linked ubiquitin chains that serve as a docking platform for do

268 ubiquitin ligases responsible for catalysing ubiquitin chains that surround intracellular bacteria ar

269 mitotic regulators by assembling K11-linked ubiquitin chains, the formation of which is initiated by

270 ant interacted with Vif and were modified by ubiquitin chains, the latter remained more resistant to

271 gulates I-kappaB degradation by removing its ubiquitin chain, thus promoting the deubiquitinated I-ka

272 new chemical approaches to covalently attach ubiquitin chains to a protein substrate through its Cys

273 USP11 can deubiquitinate hybrid SUMO-ubiquitin chains to counteract RNF4.

274 ulatively involved both direct attachment of ubiquitin chains to DMalpha and a functional tyrosine-ba

275 existing conformations, but may also remodel ubiquitin chains to hydrolyse the isopeptide bond.

276 ike TNF, IL-1 requires K63-linked and linear ubiquitin chains to recruit NEMO into higher-order compl

277 ponsive E3 ligase RNF8 conjugates K63-linked ubiquitin chains to tankyrase 1, while in G1 phase such

278 ligase-1 (HOIL-1L) and conjugate K48-linked ubiquitin chains to the catalytic RING-between-RING doma

279 gates and damaged organelles are tagged with ubiquitin chains to trigger selective autophagy.

280 DUBs present specificity toward different ubiquitin chain topologies and are crucial for recycling

281 es a cellular recognition mechanism for this ubiquitin chain type.

282 Several ubiquitin chain types have remained unstudied, mainly be

283 the connectivity between subunits, different ubiquitin chain types trigger distinct outputs, as seen

284 own enzyme complex capable of forming linear ubiquitin chains under native conditions to date is the

285 However, upon binding a ubiquitin chain, Usp14 enhances proteasomal degradation

286 Ubiquitin chains versus monomeric ubiquitin were superio

287 by producing unanchored lysine (K)63-linked ubiquitin chains via the proteasomal deubiquitinating en

288 PC is modified predominantly with K63-linked ubiquitin chains when it is bound to Axin in unstimulate

289 APC is conjugated with Lys-63-linked ubiquitin chains when it is bound to Axin, but it is unc

290 ms2, thereby stimulating formation of Lys-63 ubiquitin chains, whereas the related RNF168 RING domain

291 njugating enzyme to a substrate or a growing ubiquitin chain, which is mediated by E3 ubiquitin ligas

292 Remarkably, unanchored K63-ubiquitin chains, which are not conjugated to any target

293 SHARPIN conjugated with Lys63 (K63)-linked ubiquitin chains, which led to inhibition of the associa

294 within the IRIF core enables degradation of ubiquitin chains, which promotes loss of 53BP1.

295 e of individual components, including linear ubiquitin chains-which allows for the remarkable versati

296 How RING-E3s can build polymeric ubiquitin chains while binding substrates and E2s at def

297 ly compared proteasomal processing of Lys-63 ubiquitin chains with that of the canonical proteolytic

298 ion depends on the interaction of K29-linked ubiquitin chains with two N-terminal loops of Ufd2p.

299 ave implications on the ease of synthesis of ubiquitin chains with varying lengths and types for stru

300 s also suggested roles for mixed or branched ubiquitin chains, yet without a method to monitor endoge