ライフサイエンスコーパス: ubiquitylated

1 on and have mapped the sites on which LAT is ubiquitylated.

2 all three subunits of the 9-1-1 complex are ubiquitylated.

3 CRL4(Cdt2) is recruited and the substrate is ubiquitylated.

4 he rate at which the transcription factor is ubiquitylated.

5 n to cause hamster NAT2 to be constitutively ubiquitylated.

6 y, but not all, UIM-containing proteins were ubiquitylated.

7 tment was able to interact with MDM2 and was ubiquitylated.

8 ifying factor that recruits the target to be ubiquitylated.

9 is revealed that H2AX is both sumoylated and ubiquitylated.

10 levels of polysumoylated Nrf2 that was also ubiquitylated.

11 effector-binding loop, and is GTP-bound and ubiquitylated.

12 n E3 ligase MDM2 and, upon DNA damage, is de-ubiquitylated.

13 RPA is hyper-phosphorylated and ubiquitylated after DNA damage.

14 tb1p and Btb2p are stable proteins, Btb3p is ubiquitylated and degraded in a Pcu3p-dependent manner.

15 , PIF3 was also rapidly phosphorylated, poly-ubiquitylated and degraded in response to blue light.

16 ow that fission yeast S phase cyclin Cig2 is ubiquitylated and degraded via both the SCF and the APC/

17 ek2A, which directly binds the APC/C, can be ubiquitylated and destroyed in Fizzy/Cdc20-depleted Xeno

18 Emi1 accumulates before mitosis and is ubiquitylated and destroyed in mitosis, independent of t

19 s carbon source transporters are known to be ubiquitylated and endocytosed when glucose-starved cells

20 The mutant receptors were poorly ubiquitylated and exhibited decreased association with t

21 the absence of Pma1-D378N, wild-type Pma1 is ubiquitylated and it undergoes internalization from the

22 of the activity trajectories originated from ubiquitylated and non-ubiquitylated FBH1 molecules revea

23 ve for ATP-dependent in vitro degradation of ubiquitylated and non-ubiquitylated proteins and did not

24 hat neuronal nitric-oxide synthase (nNOS) is ubiquitylated and proteasomally degraded.

25 nant catalytic light chain (LC) of BoNT/E is ubiquitylated and rapidly degraded in cells.

26 ied proteomes using parallel quantitation of ubiquitylated and SUMOylated peptides.

27 compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to gen

28 phyA is ubiquitylated and targeted for proteasome-mediated degra

29 H2B was shown to be ubiquitylated and then deubiquitylated at the GAL1 core

30 howed that the mutant protein was apparently ubiquitylated and was preferentially located in the cyto

31 We now show that Pma1-D378N is ubiquitylated, and it heterooligomerizes with wild-type

32 p12(I) is ubiquitylated, and mutations of its unique carboxy-termi

33 a Derlin-1- and p97-dependent fashion, poly-ubiquitylated, and targeted for proteasomal degradation.

34 We show that both Phm5p and Cps1p are ubiquitylated, and that in a doa4 mutant, which has redu

35 find a pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub) outside of canon

36 of mouse glomeruli revealed that podocin is ubiquitylated at two lysine residues.

37 UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulati

38 R1 and AFB1-3, which indicates that they are ubiquitylated by a CUL1-dependent E3 ligase.

39 be a substrate of AtCHIP, because it can be ubiquitylated by AtCHIP in vitro and because the activit

40 FtsH1 can be ubiquitylated by AtCHIP in vitro, and the steady-state l

41 tion of the APC, as the N-terminal domain is ubiquitylated by purified APC in vitro.

42 Here, we show that the FACT subunit Spt16 is ubiquitylated by San1 (an E3 ubiquitin ligase) and degra

43 BOK is ubiquitylated by the AMFR/gp78 E3 ubiquitin ligase compl

44 During mitosis, CYCLIN A and CYCLIN B are ubiquitylated by the anaphase-promoting complex/cyclosom

45 roteins not involved in a repair complex are ubiquitylated by the E3 ubiquitin ligase CHIP and subseq

46 to be an hsp90 "client" protein that can be ubiquitylated by the hsp70-dependent E3 ubiquitin ligase

47 We show that MCM3 is ubiquitylated by the KEAP1-CUL3-RBX1 complex in cells an

48 Under basal conditions, NRF2 is continuously ubiquitylated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase

49 ion of Rtt101 or mutating H3 lysine residues ubiquitylated by the Rtt101(Mms1) ligase impairs nucleos

50 the G2 phase of the cell cycle, and CP110 is ubiquitylated by the SCF(Cyclin F) ubiquitin ligase comp

51 ort that the central player in HR, RAD51, is ubiquitylated by the SCF(FBH1) complex.

52 In general, G(1) and S phase cyclins are ubiquitylated by the SCF, whereas ubiquitylation of mito

53 alpha2) is a short-lived protein known to be ubiquitylated by two distinct pathways, one involving th

54 Both the internalization of ubiquitylated cargo from the plasma membrane and its sor

55 Ubiquitylated cargo proteins are recognized by ubiquitin

56 ted by ubiquitylation and, unlike sorting of ubiquitylated cargo, is insensitive to functional inhibi

57 o the class E compartment, which accumulates ubiquitylated cargoes in cells lacking ESCRT components.

58 action of UIM-containing proteins with other ubiquitylated cellular proteins.

59 quitylated CFTR, whereas p97 associated with ubiquitylated CFTR.

60 f its paralog RNF169, but how they recognize ubiquitylated chromatin is unknown.

61 ing of various cellular substrates including ubiquitylated chromatin proteins.

62 rgeted ATP-dependent segregase that extracts ubiquitylated client proteins from membranes, protein co

63 mpanied by a reduced ability of HSJ1 to bind ubiquitylated clients and to exert its chaperone activit

64 The Cdc48/p97 segregase then associates with ubiquitylated CMG, leading rapidly to helicase disassemb

65 In the absence of CRL2(Lrr1), Mcm7 is not ubiquitylated, CMG unloading is inhibited, and a large s

66 Ubiquitylated DAT was detected in the plasma membrane us

67 that the cellular levels of Pol beta and its ubiquitylated derivative are modulated by Mule and ARF a

68 diated through specific association with K63 ubiquitylated, detergent insoluble, higher order mutant

69 ion of E6 protein stability and suggest that ubiquitylated E6 could be a substrate for USP15 ubiquiti

70 Unmodified alpha2 also bound to and was ubiquitylated efficiently by Slx5-Slx8.

71 Expression of ubiquitylated Egfr (Egfr-Ub) was increased in Min/+ ente

72 strumental in the extraction and delivery of ubiquitylated ER proteins for proteasomal degradation.

73 quitylates ERCC1 in vitro, and the levels of ubiquitylated ERCC1 are markedly enhanced in USP45 knock

74 Reversal of these defects requires ubiquitylated FANCI-FANCD2.

75 tories originated from ubiquitylated and non-ubiquitylated FBH1 molecules revealed that ubiquitylatio

76 misfold and would need to be recognized and ubiquitylated for degradation.

77 clin-dependent kinase inhibitor p27(Kip1) is ubiquitylated for proteasomal degradation in Wnt10b-indu

78 ng domain provides enhanced affinity for the ubiquitylated form of PCNA and is essential for in vivo

79 ubstrate Sic1 stabilized Sic1 in vivo in its ubiquitylated form.

80 ed in response to DNA damage, and to isolate ubiquitylated forms of RNA polymerase II.

81 n WT mice, coincident with the appearance of ubiquitylated forms of the protein.

82 preferential physical interactions with the ubiquitylated forms of the replicative sliding clamp, pr

83 rotein (VCP)/p97 promotes the degradation of ubiquitylated GS, resulting in its accumulation in cells

84 nstrated that the HoxC5 gene is regulated by ubiquitylated H2A in HeLa cells and that ubiquitylated H

85 by ubiquitylated H2A in HeLa cells and that ubiquitylated H2A is localized on 5' regulatory regions

86 BCOR complex components and mono-ubiquitylated H2A localize to BCL6 targets, indicating t

87 We establish that RNF169 binds to ubiquitylated H2A-Lys13/Lys15 in a manner that involves

88 s distinct from that by which 53BP1 binds to ubiquitylated H2A-Lys15 highlighting the diversity in si

89 We additionally show that both Rad6 and ubiquitylated H2B are absent from two regions of transcr

90 region, and Spt16 regulates the formation of ubiquitylated H2B both globally and at the GAL1 gene.

91 35 into COMPASS, and increasing the level of ubiquitylated H2B in H2A tail mutants suppresses the H3K

92 ition of p97 function affected the amount of ubiquitylated H2B in muscle cells.

93 ombinant chromatin template containing fully ubiquitylated H2B to gain valuable insights.

94 Here we demonstrate, using chemically ubiquitylated H2B, a direct stimulation of hDot1L-mediat

95 used uH2A, as well as a previously generated ubiquitylated H2B, in chaperone-coupled nucleosome stabi

96 ne-associated and overall cellular levels of ubiquitylated H2B.

97 y for the semisynthesis of site-specifically ubiquitylated histone H2A (uH2A).

98 cruited to sites of DNA damage by binding to ubiquitylated histone H2A.

99 2 promoter and hydrolyzes monoubiquitin from ubiquitylated histone H2B and blocks transcription of th

100 ays to demonstrate that the direct effect of ubiquitylated histones on nucleosomal stability is in fa

101 , bacterially expressed CYP2E1(Delta3-29) is ubiquitylated in a CHIP-dependent manner when it is incu

102 olog, budding yeast Polymerase eta itself is ubiquitylated in a manner dependent on its ubiquitin-bin

103 slation in vitro, ribosome-stalled NSPs were ubiquitylated in an Ltn1-dependent manner, while still r

104 w that the TCR:CD3 complex is constitutively ubiquitylated in immature double positive (DP) thymocyte

105 ted in plants (EIN3 and JAZ6) or shown to be ubiquitylated in other eukaryotes (ribosomal subunits, e

106 nly a few have been conclusively shown to be ubiquitylated in planta.

107 ded those predicted by genetic studies to be ubiquitylated in plants (EIN3 and JAZ6) or shown to be u

108 a model in which only a fraction of pol II, ubiquitylated in response to cisplatin damage of DNA, di

109 resin to show that the Def1 protein becomes ubiquitylated in response to DNA damage, and to isolate

110 ngly, tau (full-length or truncated) was not ubiquitylated in situ.

111 Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating

112 ed E3-ligase activity in vitro and also self-ubiquitylated in the presence of ubiquitin-activating an

113 in isoforms that are both phosphorylated and ubiquitylated in the yeast Saccharomyces cerevisiae, ide

114 Specifically, we show that Siz1 can be ubiquitylated in vivo and is degraded in an Slx5-depende

115 Here we show that FRQ is likely ubiquitylated in vivo, and its proper degradation requir

116 substrates, the UBR1/2-bound RECQL4 was not ubiquitylated in vivo, and was a long-lived protein in H

117 ly phosphorylated forms of Zip1, and Zip1 is ubiquitylated in vivo, indicating that Zip1 is a substra

118 (Cdk9), the catalytic subunit of P-TEFb, is ubiquitylated in vivo.

119 demonstrate that TIN2 binds to Siah2 and is ubiquitylated in vivo.

120 ed polyglutamine diseases is the presence of ubiquitylated inclusion bodies, which sequester various

121 retained in the ER and can form cytoplasmic ubiquitylated inclusions.

122 Substrates in rad4Delta cells are ubiquitylated, indicating that Rad4 regulates a postubiq

123 ytes was associated with the accumulation of ubiquitylated intermediates of troponin I and was protea

124 d knockdown of USP8 enhanced accumulation of ubiquitylated KCa3.1, thereby inhibiting channel degrada

125 Arginine substitution of the ubiquitylated lysine impairs this inactivation mechanism

126 We identified three ubiquitylated lysine residues and showed that DNA ligase

127 hibition of proliferation and replacement of ubiquitylated lysine residues reduced the in vitro ubiqu

128 lation of ubiquitylated yeast SOD1 and other ubiquitylated mammalian proteins activate ExoU.

129 Ubiquitylated MDC1 associates with chromatin before and

130 m arrangement of UBDs is required to protect ubiquitylated Met4 from degradation and enables direct i

131 Once internalized, ubiquitylated MHC class I is targeted to the late endocy

132 t of p21WAF1/CIP1 turn-over and show how non-ubiquitylated molecules might bypass the 19S regulator o

133 through the canonical pathway by extracting ubiquitylated NRF2 from the KEAP1-CUL3 E3 complex, with

134 esolution model of human RNF169 binding to a ubiquitylated nucleosome, and validate it by electron cr

135 he diversity in site-specific recognition of ubiquitylated nucleosomes.

136 is study, we show that a strain unable to be ubiquitylated on H2B (K123R) is still proficient for H3

137 domain (CTD) of PTCH1 interacts with and is ubiquitylated on K1413 by the E3 ubiquitin-protein ligas

138 Furthermore, we show that USP4 is ubiquitylated on multiple sites including those on cyste

139 und at high affinity and is released only in ubiquitylated or partially disassembled forms.

140 lysine residues overlap with those that are ubiquitylated, our results suggest that one major functi

141 cleolar isolation revealed a distribution of ubiquitylated p53 that supports these findings.

142 This generates the apoptotically active non-ubiquitylated p53.

143 c finger (UBZ) domain, helps recruit FAN1 to ubiquitylated PCNA accumulated at stalled forks.

144 rtan, a protein that specifically recognizes ubiquitylated PCNA and plays an important role in cellul

145 We observed increased levels of ubiquitylated PCNA and significantly lower mutation freq

146 ing FAN1 activities and its interaction with ubiquitylated PCNA may offer therapeutic opportunities f

147 s1 as a UBZ-dependent downstream effector of ubiquitylated PCNA suggests an explanation for the ambiv

148 In vitro, Spartan engages ubiquitylated PCNA via both a PIP box and a UBZ domain.

149 Surprisingly, while Spartan is recruited by ubiquitylated PCNA, knockdown of Spartan compromised chr

150 Thus, as a "reader" of ubiquitylated PCNA, Spartan promotes an unexpected feed-

151 pla3(148M/M) mice, and only trace amounts of ubiquitylated PNPLA3 were seen in these animals.

152 ckdown of USP47 causes an increased level of ubiquitylated Pol beta, decreased levels of Pol beta, an

153 and cisplatin indicated that the undegraded ubiquitylated polymerase was mostly unbound or only loos

154 proteasomes by concurrent interactions with ubiquitylated proteasome subunits/targets and lipidated

155 by an absence of eif2alpha phosphorylation, ubiquitylated protein accumulation, and proteotoxicity.

156 ) and was associated with elevated levels of ubiquitylated protein and stabilization of the Ub-depend

157 ESCRT-I complex, which functions in sorting ubiquitylated protein cargoes into multivesicular bodies

158 on degradation substrate exhibited decreased ubiquitylated protein degradation.

159 SIL1 function in mouse results in ER stress, ubiquitylated protein inclusions, and degeneration of sp

160 o tightly regulated and determines whether a ubiquitylated protein is destroyed or deubiquitylated an

161 ata 1) demonstrate that the enhanced pool of ubiquitylated protein observed during neuritogenesis in

162 roteins play an important role in delivering ubiquitylated protein substrates to the proteasome for d

163 Through mass spectrometric analysis of ubiquitylated proteins affinity-purified from etiolated

164 n vitro degradation of ubiquitylated and non-ubiquitylated proteins and did not display substrate-sti

165 gnaling (Sts)-2 is associated with c-Cbl and ubiquitylated proteins and has been implicated in the re

166 Moreover, JS-K decreases total ubiquitylated proteins and increases p53 levels, which i

167 Ubiquitylated proteins are degraded by the 26 S protease

168 Ubiquitylated proteins are directed into a large number

169 It has been suggested that ubiquitylated proteins are directed to inclusion bodies

170 Ubiquitylated proteins are typically recognized using on

171 rotein sorting (VPS) pathway, where it binds ubiquitylated proteins as they are sorted into vesicles

172 tein recognition domain that is used to bind ubiquitylated proteins during vacuolar protein sorting,

173 aproteasomal ubiquitin receptor that targets ubiquitylated proteins for degradation.

174 andem ubiquitin-binding domains that protect ubiquitylated proteins from degradation.

175 n ubiquitylation, and to effectively protect ubiquitylated proteins from the action of DUBs and the p

176 Interestingly, neither of these two ubiquitylated proteins interacts with Nedd4 through PY-m

177 in of Vps36/ESCRT-II also inhibit sorting of ubiquitylated proteins into the yeast vacuole.

178 Intracellular deposition of aggregated and ubiquitylated proteins is a prominent cytopathological f

179 The ability to bind and cleave ubiquitylated proteins is consistent with AT3 playing a

180 a dynamic balance between free ubiquitin and ubiquitylated proteins is key to UPS function, the mecha

181 whereas the decision to degrade or sequester ubiquitylated proteins is mediated by the Hsp70s.

182 These effects resulted in an accumulation of ubiquitylated proteins leading to protein aggregation an

183 TUG depletion had no marked effect on total ubiquitylated proteins or p97 localization, TUG overexpr

184 It binds ubiquitylated proteins through its Ubiquitin Interacting

185 ut the mechanisms that govern recruitment of ubiquitylated proteins to IBs are not well understood.

186 ociated (UBL/UBA) proteins that help shuttle ubiquitylated proteins to the 26S proteasome for breakdo

187 ors that aid in the handling and delivery of ubiquitylated proteins to the proteasome.

188 nickel chelate-affinity chromatography, the ubiquitylated proteins were trypsinized, separated by tw

189 asome inhibitors, which block degradation of ubiquitylated proteins, are widely used in research, and

190 chains, which are detectable on endogenously ubiquitylated proteins, dampen cellular turnover of thes

191 ssue-dependent reductions of diubiquitin and ubiquitylated proteins, increased deubiquitylation activ

192 to the licensing factor CDT-1 and additional ubiquitylated proteins, thus promoting CDC-48/p97-depend

193 interaction of the endocytic machinery with ubiquitylated proteins.

194 interaction of VCP with Ufd1 as well as with ubiquitylated proteins.

195 at has a critical role in the degradation of ubiquitylated proteins.

196 ted in accumulation of high molecular weight ubiquitylated proteins.

197 n, negatively regulates cellular turnover of ubiquitylated proteins.

198 ss1 cause growth defects and accumulation of ubiquitylated proteins.

199 ectrophoresis to identify phosphorylated and ubiquitylated receptor intermediates.

200 Required for Transport (ESCRT), which target ubiquitylated receptors to intra-lumenal vesicles (ILVs)

201 These misfolded channels are poly-ubiquitylated, recognized by p97, and targeted for prote

202 itro, and ubp3 cells have elevated levels of ubiquitylated RNAPII in vivo.

203 In agreement with this, ubiquitylated RNAPII is hypophosphorylated at serine 5 i

204 ated T cells and stabilized UBR5, which then ubiquitylated RORgammat in response to TGF-beta signalli

205 ed ubiquitin ligase activity, and similarly, ubiquitylated Siz1p demonstrates reduced SUMO ligase act

206 recognition protein Rad4 and an increase in ubiquitylated species of Rad23.

207 Ubiquitylated species of the transducin gamma-subunit (T

208 d-light treatment, PhyA becomes the dominant ubiquitylated species, with ubiquitin attachment sites m

209 chain, and provide evidence that it and the ubiquitylated substrate are committed to each other afte

210 The resulting multi-ubiquitylated substrate is degraded by the 26S proteasom

211 ad4, a Rad23-binding protein, also regulates ubiquitylated substrate turnover.

212 -terminal Arg of a substrate and target the (ubiquitylated) substrate to the proteasome.

213 family of proteins that preferentially binds ubiquitylated substrates and multi-Ub chains through a m

214 TUG overexpression caused an accumulation of ubiquitylated substrates and targeted both TUG and p97 t

215 Remarkably, in Ssa1/Ssa2-depleted cells, ubiquitylated substrates are sequestered into detergent-

216 lar machinery responsible for recognition of ubiquitylated substrates can make subtle distinctions be

217 19S regulatory particle (RP), which prepares ubiquitylated substrates for degradation, has remained e

218 uggesting a possible role in the transfer of ubiquitylated substrates from Cdc48 to the proteasome or

219 large network of adaptor proteins to process ubiquitylated substrates in different cellular pathways,

220 in recent years to comprehensively identify ubiquitylated substrates of a particular pathway.

221 roach, we inhibited the proteasome to "trap" ubiquitylated substrates on the SCF(FBXW11) E3 complex.

222 ubiquitylation, unfolding, and channeling of ubiquitylated substrates to the 20S proteasome.

223 ting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their deg

224 The mechanism underlying the delivery of ubiquitylated substrates to the proteasome is poorly und

225 nt domains, Rad23 serves as a bridge linking ubiquitylated substrates to the proteasome.

226 binding protein involved in the transfer of ubiquitylated substrates to the proteasome.

227 MultiDsk binds ubiquitylated substrates with unprecedented avidity, and

228 mechanisms used by the proteasome to capture ubiquitylated substrates.

229 uitin conjugates directly and so link p97 to ubiquitylated substrates.

230 SP29 displayed nonspecific reactivity toward ubiquitylated substrates.

231 quence of events prior to the degradation of ubiquitylated substrates.

232 ever, the accumulated p68 appears to be poly-ubiquitylated, suggesting a possible defect in proteasom

233 UBE3A ubiquitylated the highly conserved Lys160 residue on SIR

234 K991 becomes ubiquitylated upon oxidative DNA damage, and while CSB K

235 We demonstrate that Miranda is ubiquitylated via its C-terminal domain; removal of this

236 Because DNA ligase I has been reported to be ubiquitylated, we used a proteomic approach to map ubiqu

237 In addition, Tgamma was exclusively ubiquitylated when Tbetagamma was dissociated from Talph

238 gene expression unless the activator is mono-ubiquitylated, which protects it from the ATPases.

239 Because few ubiquitylated yeast proteins are known to be degraded by

240 We report here that a subpopulation of ubiquitylated yeast SOD1 and other ubiquitylated mammali