ライフサイエンスコーパス: ultracentrifugation

1 on, dynamic light scattering, and analytical ultracentrifugation).

2 ddition of sI-Pht, as assessed by analytical ultracentrifugation.

3 2 +/- 10 mol/mol as determined by analytical ultracentrifugation.

4 multi-angle light scattering, and analytical ultracentrifugation.

5 ro study using gel filtration and analytical ultracentrifugation.

6 ion of hydrodynamic parameters in analytical ultracentrifugation.

7 those from solution studies using analytical ultracentrifugation.

8 ules using sedimentation velocity analytical ultracentrifugation.

9 on chromatography and equilibrium analytical ultracentrifugation.

10 he CC and the NTD as monitored by analytical ultracentrifugation.

11 ter purification by sucrose density gradient ultracentrifugation.

12 cell-culture supernatant fluids by isopycnic ultracentrifugation.

13 ) ( approximately 10(6) IU/ml), even without ultracentrifugation.

14 re sacrificed and plasma MP were isolated by ultracentrifugation.

15 size exclusion chromatography and analytical ultracentrifugation.

16 confirmed to exist in solution by analytical ultracentrifugation.

17 e-detected sedimentation velocity analytical ultracentrifugation.

18 wn by fluorescence anisotropy and analytical ultracentrifugation.

19 ysis of supernatants and pellets obtained by ultracentrifugation.

20 nsmission electron microscopy and analytical ultracentrifugation.

21 dimentation-diffusion analysis by analytical ultracentrifugation.

22 butions to homodimer stability by analytical ultracentrifugation.

23 e prepared by sequential ultrafiltration and ultracentrifugation.

24 ion, using sedimentation velocity analytical ultracentrifugation.

25 n analyzed by light scattering or analytical ultracentrifugation.

26 and signal peptide binding using analytical ultracentrifugation.

27 ce energy transfer and sucrose-step gradient ultracentrifugation.

28 nal hydrodynamic information from analytical ultracentrifugation.

29 m this preparation were then generated using ultracentrifugation.

30 tions, as analyzed by sedimentation velocity ultracentrifugation.

31 ize exclusion chromatography, and analytical ultracentrifugation.

32 crystal structure is confirmed by analytical ultracentrifugation.

33 lation Kit or by differential centrifugation/ultracentrifugation.

34 Exosomes were prepared using ultracentrifugation.

35 ligonucleotide-binding assays and analytical ultracentrifugation.

36 oluble Htt-polyQ aggregates using analytical ultracentrifugation.

37 by solution NMR spectroscopy and analytical ultracentrifugation.

38 Released EVs were isolated by differential ultracentrifugation.

39 subjects or patients with CKD by sequential ultracentrifugation.

40 ical properties following iodixanol gradient ultracentrifugation.

41 ects, as well as mouse HDL, were isolated by ultracentrifugation.

42 in solution, as revealed through analytical ultracentrifugation.

43 (1) analysis of Lp(a) fractions isolated by ultracentrifugation; (2) immunoprecipitation of plasma u

44 Advances in analytical ultracentrifugation allow for the extraction of the sedi

45 tational, NMR chemical shift, and analytical ultracentrifugation analyses allowed us to precisely def

46 Analytical ultracentrifugation analyses show that the individual pe

47 Buoyant density ultracentrifugation analyses showed that NO2-Tyr(166)-ap

48 size exclusion chromatography and analytical ultracentrifugation analyses.

49 angle X-ray scattering (SAXS) and analytical ultracentrifugation analysis of the EcoP15I holoenzyme a

50 Analytical ultracentrifugation analysis reveals that the maturation

51 onal cryo-electron microscopy and analytical ultracentrifugation analysis, we determined the structur

52 In this study, we used sucrose gradient ultracentrifugation and a genome-wide microarray approac

53 vitro by fluorescence anisotropy, analytical ultracentrifugation and analytical gel filtration.

54 Analytical ultracentrifugation and circular dichroism experiments s

55 bjected to further analysis using analytical ultracentrifugation and circular dichroism.

56 Gel filtration analyses, analytical ultracentrifugation and co-immunoprecipitation experimen

57 Using ultracentrifugation and confocal microscopy, we found th

58 d several domain constructs using analytical ultracentrifugation and correlated VAI binding and PKR i

59 Analytical ultracentrifugation and crosslinking studies support the

60 arance with and without exosomes isolated by ultracentrifugation and determined exosome-induced amylo

61 of PGR in solution as measured by analytical ultracentrifugation and dynamic light scattering.

62 Our analytical ultracentrifugation and electron microscopy show differe

63 Here, we use analytical ultracentrifugation and electron microscopy to further c

64 Mutational analysis coupled with analytical ultracentrifugation and equilibrium denaturations showed

65 A series of ultracentrifugation and Exoquick isolation kit were firs

66 Analytical ultracentrifugation and fluorescence anisotropy methods

67 of endophilin both in vitro using analytical ultracentrifugation and fluorescence anisotropy, and in

68 rat brain alphabeta-tubulin using analytical ultracentrifugation and fluorescence anisotropy, observi

69 Assays based on analytical ultracentrifugation and fluorescence binding indicate th

70 TP protofilaments was observed by analytical ultracentrifugation and fluorescence correlation spectro

71 293T cell EVs isolated by flotation gradient ultracentrifugation and from exosomes containing the tet

72 Analytical ultracentrifugation and hydrogen deuterium exchange mass

73 ography binding assays as well as analytical ultracentrifugation and isothermal titration calorimetry

74 m purified human platelets, were isolated by ultracentrifugation and labeled with biotin or PKH67.

75 Analytical ultracentrifugation and liquid chromatography-ultraviole

76 ion of the Cpn60.2 proteins using analytical ultracentrifugation and mass spectroscopy.

77 Here, we demonstrate using sucrose gradient ultracentrifugation and methyl-beta-cyclodextrin treatme

78 inker with sedimentation velocity analytical ultracentrifugation and NMR spectroscopy reveals that th

79 okine-type dimer as determined by analytical ultracentrifugation and NMR.

80 se the enzyme and its subunits by analytical ultracentrifugation and obtain low-resolution structural

81 Ultracentrifugation and size exclusion chromatography in

82 m spectra, as well as equilibrium analytical ultracentrifugation and size exclusion chromatography.

83 We determined by means of analytical ultracentrifugation and small angle x-ray scattering ana

84 e of truncated M. sexta sGC using analytical ultracentrifugation and small-angle X-ray scattering (SA

85 Size separation of the urine by ultracentrifugation and sodium dodecyl sulfate polyacryl

86 20 and 400 nm from human serum and FBS using ultracentrifugation and sucrose gradient centrifugation.

87 Ultracentrifugation and virion assembly assays indicated

88 tions in relation to C3b and C3u, analytical ultracentrifugation and x-ray and neutron scattering stu

89 nd temperatures was determined by analytical ultracentrifugation and X-ray and neutron scattering.

90 zinc with C3 was quantified using analytical ultracentrifugation and x-ray scattering.

91 pattern of multimers observed in analytical ultracentrifugation, and a concentration dependence of a

92 tein/lipid ratio shown by isopycnic gradient ultracentrifugation, and are small in size with a mean d

93 gle particle electron microscopy, analytical ultracentrifugation, and bio-layer interferometry, we pr

94 Precipitation, analytical ultracentrifugation, and chemical cross-linking experime

95 amagnetic relaxation enhancement, analytical ultracentrifugation, and DEER EPR, indicate that the tra

96 tallography, solution scattering, analytical ultracentrifugation, and electron microscopy we determin

97 oblot, immunoprecipitation, density gradient ultracentrifugation, and enzyme-linked immunosorbent ass

98 NMR, analytical ultracentrifugation, and fluorescence studies suggest th

99 tures, Ca(2+) binding capacities, analytical ultracentrifugation, and light-scattering profiles of th

100 ntified by NMR spectroscopy, SPR, analytical ultracentrifugation, and microcalorimetry glycopeptides

101 entary surface plasmon resonance, analytical ultracentrifugation, and native mass spectrometry experi

102 tical gel filtration, sedimentation-velocity ultracentrifugation, and negative-stain electron microsc

103 using the fluorescence titration, analytical ultracentrifugation, and photo-cross-linking techniques.

104 egates and Abeta monomers using differential ultracentrifugation, and purifying them >6000 fold by du

105 sm and fluorescence spectroscopy, analytical ultracentrifugation, and semi-native gel electrophoresis

106 also released into the medium and removed by ultracentrifugation, and similarly affected by warfarin.

107 tering, dynamic light scattering, analytical ultracentrifugation, and small angle X-ray scattering ea

108 graphy, dynamic light scattering, analytical ultracentrifugation, and small angle x-ray scattering ex

109 rom small-angle x-ray scattering, analytical ultracentrifugation, and steady-state and rapid-reaction

110 easured after vertical spin density-gradient ultracentrifugation, and triglycerides were directly mea

111 higher sedimentation velocities in gradient ultracentrifugations, and a longer incubation time in an

112 We have combined EM and analytical ultracentrifugation approaches to show that RECQ1 can fo

113 ent, sonication and sucrose density-gradient ultracentrifugation are subsequently used to isolate the

114 pared with direct binding measurements using ultracentrifugation as a reference.

115 in LDL cholesterol, as measured by means of ultracentrifugation, at week 52.

116 id testing by vertical spin density gradient ultracentrifugation (Atherotech, Birmingham, Alabama) fr

117 profiling by vertical spin density gradient ultracentrifugation (Atherotech, Birmingham, Alabama) fr

118 Here, we show how two techniques, analytical ultracentrifugation (AUC) and total organic carbon analy

119 Analytical ultracentrifugation (AUC) has proven to be a powerful to

120 Sedimentation velocity (SV) analytical ultracentrifugation (AUC) is a classic technique for the

121 Sedimentation equilibrium (SE) analytical ultracentrifugation (AUC) is a gold standard for the rig

122 Analytical ultracentrifugation (AUC) is especially useful for the c

123 Analytical ultracentrifugation (AUC) studies of formation of the N-

124 ission Electron Microscopy (TEM), Analytical Ultracentrifugation (AUC), and UV/Vis spectroscopy.

125 on agrees quantitatively with our analytical ultracentrifugation-based measurements and previously pu

126 r dimers; we report here that, by analytical ultracentrifugation, both oxidized and reduced E. coli B

127 ne has shown that isopycnic density gradient ultracentrifugation can produce structurally and electro

128 ochron iButton) that can be inserted into an ultracentrifugation cell assembly and spun at low rotor

129 Additionally, analytical ultracentrifugation characterisation of rE2/E3BP, rE2 (f

130 Sedimentation velocity analytical ultracentrifugation combines relatively high hydrodynami

131 These data and analytical ultracentrifugation compaction analyses are used herein

132 d molecular weights determined by analytical ultracentrifugation confirm the SAXS model.

133 Analytical ultracentrifugation confirmed the presence of Ig domain

134 veolar lavage fluid of asthmatic subjects by ultracentrifugation contained TF.

135 Ultracentrifugation converted the purified 3F7.A10 mAb i

136 mers to a single compact dimer by analytical ultracentrifugation, coupled with a >30 A decrease in ma

137 tive polyacrylamide gel electrophoresis, and ultracentrifugation data show that the dimer is in equil

138 When combined with analytical ultracentrifugation data, these results suggest that Sin

139 s validated against gold-standard analytical ultracentrifugation data.

140 this protocol describes sample preparation, ultracentrifugation, data acquisition, and data analysis

141 LDL was isolated from venous plasma by ultracentrifugation, delipidated, digested, and analyzed

142 Evidence from NMR and analytical ultracentrifugation demonstrates that the carnosic acid

143 The recent application of density gradient ultracentrifugation (DGU) for structural sorting of sing

144 ation of (13)C labeling and density gradient ultracentrifugation (DGU) to produce an array of (13)C-l

145 and metallic SWCNTs through density gradient ultracentrifugation (DGU).

146 nts (FtsZ-GTP) as demonstrated by analytical ultracentrifugation, dynamic light scattering, fluoresce

147 ns below 30 muM, as determined by analytical ultracentrifugation, dynamic light scattering, size excl

148 d as a tetramer, as determined by analytical ultracentrifugation, electron microscopy, and electrospr

149 methods that includes sedimentation velocity ultracentrifugation, electron microscopy, and hydrodynam

150 Using surface plasmon resonance, ultracentrifugation, ELISA, and reporter cell assays, we

151 Sedimentation velocity analytical ultracentrifugation equipped with fluorescence detection

152 were synthesized and utilized in analytical ultracentrifugation experiments and demonstrate that MER

153 e bonds, NMR solution studies and analytical ultracentrifugation experiments are reported in addition

154 Kinetic and analytical ultracentrifugation experiments demonstrate that alloste

155 Analytical ultracentrifugation experiments demonstrate that CheR2 i

156 Analytical ultracentrifugation experiments indicated that Kindlin-3

157 Analytical ultracentrifugation experiments show that mature caspase

158 Circular dichroism and analytical ultracentrifugation experiments show that this core regi

159 synchrotron x-ray scattering and analytical ultracentrifugation experiments showed that the carbohyd

160 enceforth referred to as VanS) in analytical ultracentrifugation experiments to study VanS oligomeric

161 to be monomeric as determined by analytical ultracentrifugation experiments, and only single headed

162 c species, which was confirmed by analytical ultracentrifugation experiments.

163 embranes using fluorescence spectroscopy and ultracentrifugation experiments.

164 tion states by gel filtration and analytical ultracentrifugation experiments.

165 hich was further characterized by analytical ultracentrifugation experiments.

166 is in qualitative agreement with analytical ultracentrifugation experiments.

167 e detected sedimentation velocity analytical ultracentrifugation (FDS-SV).

168 Here we use analytical ultracentrifugation, fluorescence polarization and NMR-b

169 ptor (hPVR) using various techniques such as ultracentrifugation, fluorescence-activated cell sorting

170 icle and dissolved Ag fractions (ICPMS after ultracentrifugation), for the characterization of Ag-NP

171 Ultracentrifugation fractionation experiments revealed t

172 pplied to RNA extracted from EVs isolated by ultracentrifugation from the plasma of five healthy volu

173 er related methods (filter assay, analytical ultracentrifugation, gel electrophoresis and size-exclus

174 slightly acidic pH's as judged by analytical ultracentrifugation, gel filtration, dynamic light scatt

175 Analytical ultracentrifugation has long been considered a gold stan

176 ibrium and sedimentation velocity analytical ultracentrifugation have been employed to determine comp

177 anti-apoE immunoaffinity chromatography and ultracentrifugation in 9 patients with moderate hypertri

178 Using analytical ultracentrifugation in both sedimentation equilibrium an

179 the proteins was determined using analytical ultracentrifugation in the absence or presence of high c

180 them (>70%) are retained even after extended ultracentrifugation in the preparations of vesicle-deple

181 X3 and AM HC-HA withstands four runs of CsCl ultracentrifugation in the presence of 4 m GnHCl.

182 ues (dynamic light scattering and analytical ultracentrifugation) in an attempt to provide the basis

183 ed on sedimentation velocity (SV) analytical ultracentrifugation, in combination with a novel multiwa

184 Analytical gel chromatography and ultracentrifugation indicated tetrameric structure of th

185 mutagenesis, gel filtration, and analytical ultracentrifugation indicated that dimers form the basic

186 Analytical ultracentrifugation indicated that SR1:3, SR4:6, and SR7

187 Sedimentation equilibrium analytical ultracentrifugation indicates that 5QMe(2) is predominan

188 Sedimentation velocity analytical ultracentrifugation is a classical biophysical technique

189 Sedimentation velocity (SV) analytical ultracentrifugation is a classical biophysical technique

190 Although ultracentrifugation is commonly used for isolation of EV

191 d using SWCNTs separated by density gradient ultracentrifugation, it is found that the high-pressure

192 By analytical ultracentrifugation, large amounts of oligomers were obs

193 th placebo, evolocumab significantly reduced ultracentrifugation LDL cholesterol at 12 weeks by 30.9%

194 he primary endpoint was percentage change in ultracentrifugation LDL cholesterol from baseline at wee

195 12, LDL-C reduction measured by preparative ultracentrifugation (least squares mean [standard error

196 ercentage change from baseline to week 12 in ultracentrifugation-measured LDL cholesterol.

197 Analytical gel-filtration and ultracentrifugation measurements confirm that the protei

198 Analytical ultracentrifugation measurements suggest that aggregatio

199 Analytical ultracentrifugation measurements support a thermodynamic

200 solated through an optimized ultrafiltration/ultracentrifugation method and characterized with variou

201 ation method was compared to the widely used ultracentrifugation method for efficiency and efficacy i

202 This new generation of ultracentrifugation methods underscores a need to furthe

203 Ultracentrifugation methods, in contrast, do not introdu

204 scence anisotropy, gel shift, and analytical ultracentrifugation methods.

205 tative fluorescence titration and analytical ultracentrifugation methods.

206 by size exclusion chromatography, analytical ultracentrifugation, multiangle laser light scattering,

207 and software for multiwavelength analytical ultracentrifugation (MWL-AUC) experiments, demonstrating

208 s verified using a combination of analytical ultracentrifugation, native electrospray mass spectromet

209 termediates were characterized by analytical ultracentrifugation, native gel electrophoresis, and ele

210 amic and structural studies using analytical ultracentrifugation, NMR, and small-angle x-ray scatteri

211 We show here using analytical ultracentrifugation, NMR, size-exclusion chromatography

212 c assembly which are confirmed by analytical ultracentrifugation of both the crystallized domain and

213 Analytical ultracentrifugation of phospho-mimetic CENP-A nucleosome

214 l packing analysis, combined with analytical ultracentrifugation of Sonic Hh-GAG complexes, suggests

215 Analytical ultracentrifugation of the recombinant versions of Tp095

216 human plasma is a component of HDL either by ultracentrifugation or by lipid binding assays.

217 the need for less efficient methods such as ultracentrifugation or high-performance liquid chromatog

218 substrate for conversion, and attenuated by ultracentrifugation or incubation with SOD1 misfolding-s

219 ired purification of viral particles through ultracentrifugation or PEG precipitation, was PEG indepe

220 Using analytical ultracentrifugation, our results revealed that Asp(21) a

221 Analytical ultracentrifugation profiles of G-actin can be ascribed

222 techniques and compared them to traditional ultracentrifugation protocol.

223 ifugation, followed by a single small-volume ultracentrifugation purification step.

224 -vis absorbance, CD spectroscopy, analytical ultracentrifugation, redox potentiometry, and EPR demons

225 xclusion chromatography (SEC) and analytical ultracentrifugation, remain the default tools in charact

226 rified complexes by sucrose density gradient ultracentrifugation revealed that the three proteins for

227 lectron microscopy with data from analytical ultracentrifugation reveals the crucial role of kinetic

228 Several methods, including analytical ultracentrifugation, scanning transmission electron micr

229 Hydrodynamic analysis by analytical ultracentrifugation sedimentation velocity and native ma

230 Analytical ultracentrifugation sedimentation velocity experiments i

231 s can significantly improve density-gradient ultracentrifugation separations.

232 from both GST pulldown assays and analytical ultracentrifugation show that GstDnaB1-300 is sufficient

233 fluorescence anisotropy decay and analytical ultracentrifugation show that the iM structure has a com

234 Analytical ultracentrifugation showed concentration-dependent self-

235 Analytical ultracentrifugation showed that 1K1 and NK1 were more st

236 Analytical ultracentrifugation showed that both antibodies were pre

237 Analytical ultracentrifugation showed that both IgG4 forms were pri

238 nce (NMR) spectroscopy as well as analytical ultracentrifugation, showing that they have a weight ave

239 Analytical ultracentrifugation shows one main complex, sedimenting

240 Hsp90 dimers and oligomers were evaluated by ultracentrifugation, size-exclusion chromatography coupl

241 eltaC) is further validated using analytical ultracentrifugation, size-exclusion chromatography, NMR

242 By combining analytical ultracentrifugation, small angle x-ray scattering, and c

243 We show using analytical ultracentrifugation, small angle x-ray scattering, and e

244 ses including circular dichroism, analytical ultracentrifugation, small-angle x-ray scattering, and h

245 and fluorescence spectroscopies, analytical ultracentrifugation, splicing assays, and cell microscop

246 centration was significantly reduced when an ultracentrifugation step preceded NTA.

247 ross-linking, gel filtration, and analytical ultracentrifugation studies aimed at evaluating interact

248 Analytical ultracentrifugation studies confirmed that the ADC solut

249 ecSecA dimerization based on our analytical ultracentrifugation studies of SecA L6A and shown to for

250 Analytical ultracentrifugation studies of the SPI1-MSPbeta interact

251 Analytical ultracentrifugation studies showed that p85alpha undergo

252 Analytical ultracentrifugation studies support that MERS-CoV 3CL(pr

253 ng pull-down, gel filtration, and analytical ultracentrifugation studies, we show that the N-terminus

254 This was confirmed by analytical ultracentrifugation studies, which reveal a monomer-dime

255 -dependent circular dichroism and analytical ultracentrifugation suggest that the htt(NT) sequence, w

256 ination of X-ray crystallography, analytical ultracentrifugation, surface plasmon resonance and doubl

257 oscopy and sedimentation velocity analytical ultracentrifugation (svAUC) to undertake initial structu

258 sorting method can be combined with existing ultracentrifugation SWCNT sorting methods to produce "or

259 rcular dichroism spectroscopy and analytical ultracentrifugation techniques.

260 titration, photocrosslinking, and analytical ultracentrifugation techniques.

261 , to show by NMR spectroscopy and analytical ultracentrifugation that at biologically relevant concen

262 We show by NMR and analytical ultracentrifugation that MVN is monomeric in solution, a

263 by surface plasmon resonance and analytical ultracentrifugation, that individual HTH motifs of the B

264 Liposome suspensions were concentrated by ultracentrifugation; the pellets were reconstituted in w

265 ld-type proteins were assessed by analytical ultracentrifugation, thioflavin T binding, transmission

266 ng tissue or cell lysates using differential ultracentrifugation through sucrose gradients.

267 We have used analytical ultracentrifugation to characterize the binding of the m

268 We employed analytical ultracentrifugation to demonstrate that dematin is monom

269 of site-directed mutagenesis and analytical ultracentrifugation to derive thermodynamic parameters f

270 Here we utilize analytical ultracentrifugation to determine the thermodynamic param

271 In this study, we employed analytical ultracentrifugation to establish that four transition me

272 interactions in solution, we used analytical ultracentrifugation to measure the dimerization constant

273 We use analytical ultracentrifugation to show that purified Blt1p is a tet

274 ng size exclusion chromatography, analytical ultracentrifugation, transmission electron, and atomic f

275 EVs have traditionally been purified by ultracentrifugation (UC), however UC has limitations, in

276 gation behavior of AgNPs were examined using ultracentrifugation, ultrafiltration, and asymmetrical f

277 anotubes (SWNTs), sorted by density-gradient ultracentrifugation, undergo self-assembly using depleti

278 ermined by sedimentation velocity analytical ultracentrifugation using recombinant mouse Myo5a tail a

279 Lipid testing was performed by ultracentrifugation (Vertical Auto Profile, Atherotech,

280 Rapid ultracentrifugation was used to directly measure LDL-C c

281 Previously, analytical ultracentrifugation was utilized to demonstrate that iso

282 ography, electron microscopy, and analytical ultracentrifugation), we describe an unprecedented alpha

283 rential scanning calorimetry, and analytical ultracentrifugation, we demonstrate the AgamOBP48 dimeri

284 Using analytical ultracentrifugation, we determined a dissociation consta

285 Using fluorescence quenching and analytical ultracentrifugation, we find that apo AidB has a high af

286 gel filtration chromatography and analytical ultracentrifugation, we found that a fully functional co

287 small-angle X-ray scattering and analytical ultracentrifugation, we found that ClipCG12 adopts a lar

288 Using analytical ultracentrifugation, we found that pH-induced conformati

289 gle x-ray scattering analysis and analytical ultracentrifugation, we show that NaD1 forms dimers in s

290 mitochondrial complexes and density gradient ultracentrifugation, we show that that 3betaHSD2 formed

291 ry human DC-secreted EV (DC-EV), isolated by ultracentrifugation, were characterized for their size,

292 size-exclusion chromatography and analytical ultracentrifugation, whereas other class III nucleotidyl

293 romatography and characterized by analytical ultracentrifugation, which demonstrated monodispersity a

294 aracterization technique based on analytical ultracentrifugation, which demonstrates exceptional pote

295 raction after discontinuous sucrose gradient ultracentrifugation, which suggests that they form small

296 Sedimentation velocity analytical ultracentrifugation with fluorescence detection has emer

297 al cell fractionation using density gradient ultracentrifugation with multiplexed quantitative proteo

298 ron X-ray solution scattering and analytical ultracentrifugation, with the goal of reconstructing the

299 However, we show using analytical ultracentrifugation, X-ray crystallography, and enzyme k

300 ion structure by a combination of analytical ultracentrifugation, X-ray scattering and constrained mo