ライフサイエンスコーパス: unbind

1 ppm upfield relative to their position when unbound.

2 e just before these three-body states become unbound.

3 binding to the 70S ribosome, rather than its unbinding.

4 ents but with slower kinetics of binding and unbinding.

5 consistent with slower glutamate binding and unbinding.

6 Equilibration between the blood and unbound (18)F-FMISO was rapid in all tumors.

7 ation of Dap to pyruvate proceeds through an unbound 2-aminoacrylate (2AA) intermediate, thus contrib

8 artitioning, dissolution rate and release of unbound active drug.

9 AFP was incubated in Ab1-coated wells; unbound AFP was then washed away with Tween-20.

10 ces, KIF3A/B motors are predicted to rapidly unbind and rebind, resulting in qualitatively different

11 requires local remodeling through crosslink unbinding and and/or filament disassembly.

12 ng that roadblock circumvention involves the unbinding and rebinding of the motors.

13 rk elasticity and force-induced cross-linker unbinding and rebinding.

14 both allosteric and direct catalysis of GDP unbinding and release and GTP binding.

15 state to the transition state for unfolding/unbinding and, hence, often related to the mechanical co

16 Cell fractionation into a cytoskeleton-unbound and a cytoskeleton-anchored desmosome-containing

17 ystal structures of human IZUMO1 and JUNO in unbound and bound conformations.

18 Multiple, atomistic simulations of the unbound and bound forms of NorM in a phospholipid lipid

19 ues) and global (e.g. hinge motion), between unbound and bound proteins through distance patterns.

20 ation showed a coherent allosteric mode from unbound and bound states, respectively.

21 on the ensemble of experimentally determined unbound and bound structures.

22 olecular dynamics (MD) simulations of Ca(2+)-unbound and Ca(2+)-bound forms of Syt1.

23 al structures of ISAV hemagglutinin-esterase unbound and complexed with receptor.

24 s by flow cytometer and discriminate between unbound and fluorescent nanoparticles.

25 croscopy (cryo-EM) and crystal structures of unbound and H1-bound nucleosomes and validated these str

26 pts a conformation that is distinct from its unbound and its protein-bound conformations.

27 assessed via rigid body simulations of both unbound and ligand-bound states of the protein.

28 n status of the hydration waters both in the unbound and the bound states.

29 ic compared to the rest of the protein, both unbound and when bound to various BH3-only proteins.

30 structures in docking, 35% (6/17) when using unbound, and 12% (2/17) when using the homology-modeled

31 , blocking layer membranes are added to stop unbound antibodies from reaching the top membrane.

32 ived placebo (9 participants) (P<0.001), and unbound apixaban concentration was reduced by 9.3 ng per

33 r-IR spectra of two forms of the protein, an unbound APO form and a HOLO form (where the PDZ1 is boun

34 ring the course of a reaction, involving two unbound apo structures, one ligand-bound intermediate, a

35 ctional nucleic acid, the SEO binding to the unbound aptamer triggered perturbations of both the inte

36 he model predicts a delayed inertial protein unbinding associated with the SNARE complex assembly imm

37 ue to specific, molecular recognition, while unbound beads move along past the electrodes.

38 ominantly by an alternate pathway, where the unbinding becomes 20 times faster, and the sharp wetting

39 ocking protocol and tested on the Dockground unbound benchmark set, significantly increasing the dock

40 e transport with fingerprints of a nanoscale unbinding/binding behavior.

41 o that which is bound to IP3Rs, implies that unbound Bok is deleterious to cell viability and helps e

42 tically stabilized by binding to IP3Rs, that unbound Bok is ubiquitinated and degraded by the proteas

43 ned the high-resolution crystal structure of unbound Brag2 containing the GEF (Sec7) and membrane-bin

44 shows significant peptide unfolding and some unbinding, but no insertion.

45 e junction in a manner similar to when it is unbound, but there is no specificity in Mg(2+) binding f

46 se-8 activation is regulated by the ratio of unbound c-FLIPL/S to procaspase-8, which determines comp

47 Thus, unbound cAMP maintains the PDE8-RIalpha complex while be

48 channel facilitates processive hydrolysis of unbound cAMP.

49 d by a natural equilibrium between bound and unbound CENH3 (and its chaperones or binding proteins).

50 A transition in which the effect of unbinding changes from inhibitory to excitatory as subst

51 lectron and hole pairs, called excitons, and unbound charge carriers is a key cross-cutting issue in

52 with minimal risk of accumulating phototoxic unbound chlorophylls.

53 proved unbound drug concentration with lower unbound clearance.

54 at the RQC complex also exists as a ribosome-unbound complex during the escort of aberrant peptides t

55 e are effective when administered in vivo at unbound concentrations consistent with their in vitro ce

56 in a manner that is proportional to the free/unbound concentrations of the drug, thereby establishing

57 regulatory proteins adopt distinct bound and unbound conformations, and use this structural flexibili

58 Significantly, Na(+) binding (and unbinding) consistently involves a transient binding to

59 es to selectively quench the fluorescence of unbound coralyne but not that of coralyne bound to c-di-

60 Moreover, after unbinding, CTCF quickly rebinds another cognate site unl

61 Concentrations of unbound dabigatran remained below 20 ng per milliliter a

62 Analysis of the unbinding data revealed a most probable interaction forc

63 For truly unbound docking, the respective success rates of ITScore

64 tin-bound Drp1 is significantly increased by unbound Drp1.

65 ro cell potency as a consequence of improved unbound drug concentration with lower unbound clearance.

66 on spatial and temporal mapping of bound and unbound drug distribution.

67 Seminal protein-unbound DTG concentrations exceeded the in vitro 50% inh

68 study paves the way to investigations on the unbinding dynamics of more complex p38 inhibitors and ot

69 blies to reveal and characterize binding and unbinding dynamics within such assemblies.

70 We present a structural model of the Ca(2+)-unbound EF-SAM domain that is consistent with a wide ran

71 Allowing different unbinding energies not only has equilibrium effects on t

72 lapse to a universal curve regardless of the unbinding energies used or the identity of the dissociat

73 The unbinding energy per bond was found to be on the order o

74 LL4 catalyzed methylation of H3K4 at distant unbound enhancers via chromatin looping, which identifie

75 we report three crystal structures of SwaI: unbound enzyme, a DNA-bound complex with calcium ions; a

76 hibits slow dynamics that fit with a binding-unbinding equilibrium.

77 In essence, NECEEM is used to measure the unbound equilibrium fraction, R, for the binder with a k

78 site, but not Na2, and, consistently, sodium unbinding/escape to the extracellular (EC) region first

79 dition of excess unbound RLC, but not excess unbound essential light chain, competes with liposome bi

80 were obtained by Poisson analysis of single unbinding event peaks, yielding values of -0.75 +/- 0.04

81 se timing sequence of stochastic binding and unbinding events allows one receptor to provide informat

82 tochastic process of innumerable binding and unbinding events between antibodies and the multiple bin

83 nitor individual ligand:receptor binding and unbinding events in space and time by single-molecule im

84 We showed that the collective frequency of unbinding events in WT-RBCs is not significantly differe

85 Single unbinding events observed at higher surface delays were

86 Explore is able to observe millisecond-scale unbinding events using many nanosecond-scale trajectorie

87 ite the highly dynamic nature of binding and unbinding events, under fast loading rates relevant to T

88 nd exhibit dramatically slower dynamics than unbound Fat4 and Ds1.

89 a priori not clear if they form a plasma of unbound fermionic particles or a gas of composite bosons

90 Unbinding follows the inverse sequence of events with th

91 tle is known on how cells can modulate their unbinding for regulation.

92 proteins on the RBC surface and measures the unbinding force between BCAM/Lu and LAMA5.

93 and SS-RBC membranes, as well as the median unbinding force between ICAM-4 and alphavbeta3.

94 However, the unbinding force between ICAM-4 and the corresponding lig

95 ression of active BCAM/Lu receptors, a lower unbinding force to LAMA5, and insignificant stimulation

96 on cells, as measured by an increase in the unbinding force.

97 Unbinding forces between ligands and SGLT1 were recorded

98 g on the direction of force application, the unbinding forces can more than triple.

99 s limited as a 3D structure of any RE in the unbound form is not available yet.

100 mechanism for the catalyst in the bound and unbound form, whereas no significant production of CO wa

101 the structural differences between bound and unbound forms allow dehydrins to act as molecular shield

102 and the detailed structure of the bound and unbound forms are not known.

103 te moiety interconverts between Fc-bound and unbound forms, enabling enzymatic modification of the gl

104 x-ray crystal structures of ligand-bound and unbound forms, the activation of the erythropoietin rece

105 are normally obscured by large fractions of unbound FPs.

106 ar concentrations in brain tissue, while the unbound fraction in brain homogenate was around 1.5%.

107 rug released from the NP mirrors that of the unbound, free-drug.

108 een less studied, during which the two cAMPs unbind from the regulatory subunit to allow association

109 n complex that must continuously bind to and unbind from transiently exposed template strands during

110 he allosteric site sterically hinders ligand unbinding from the central site, providing an explanatio

111 , and it reverses within seconds of arrestin unbinding from the transient binding mode.

112 clusters whose constituent stars have become unbound from one another and spread across the Galactic

113 ed by one C atom simply from the bound to an unbound functionality and thus able to discriminate betw

114 by doing so, increases cytoplasmic levels of unbound GK.

115 remove the steric constraints on ligand, the unbinding happens predominantly by an alternate pathway,

116 xperiments confirmed the disordered state of unbound HMGA2.

117 lyses of hp53 target genes demonstrated that unbound hp53 was absolutely required for activation of t

118 c2 to recognize the bc1 complex and bind or unbind in a redox state-dependent manner.

119 bited Vn to supported lipid bilayers, and to unbinding in freestanding lipid vesicles.

120 Further, since single particles are detected unbound in flow, the technique can be broadly applied to

121 Fraction unbound in plasma (fup) and intrinsic hepatic clearance

122 cules (such as switching between binding and unbinding) in the in vivo environment of living cells.

123 ing the specific DNA with the nonspecific in unbound, intermediate, and associated states, we found t

124 insoluble iron), ultrafiltration (to remove unbound iron) and lyophilisation (to attain in dry form)

125 FkappaB is the strongest DNA binder, and its unbinding is controlled kinetically by molecular strippi

126 associated rate constants of protein-ligand unbinding is of great practical importance in drug desig

127 are captured by computing the probability of unbound kinesin being at given locations.

128 Experimental approaches to binding and unbinding kinetics are nowadays available, but we still

129 at they show unusual concentration-dependent unbinding kinetics from chromosomal recognition sites in

130 ision single-molecule tracking, we study the unbinding kinetics from DNA of two metal-sensing transcr

131 Unexpectedly, their unbinding kinetics further varies with the extent of chr

132 trap to probe the velocity, run length, and unbinding kinetics of mouse KIF3A/B under various loads

133 In a regime of slow DNA-binding/unbinding kinetics, spontaneous switching occurs relativ

134 In contrast, in a regime of fast DNA-binding/unbinding kinetics, switching occurs rarely and is drive

135 tion initiation; it modulates RNAP's binding-unbinding kinetics, without allowing interconversions be

136 designing new drugs with engineered binding/unbinding kinetics.

137 Three-dimensional diffusion constants for unbound LacI computed from simulations closely match tho

138 ted from simulations closely match those for unbound LacI in vivo reported in the literature.

139 A possibly better picture of unbound LacI motion is that of gated diffusion, where DN

140 oggle between closed, Lewis adduct and open, unbound Lewis pair structures.

141 e of ligand binding and the concentration of unbound ligand, leading to a model-independent binding c

142 Comparison of gp41-bound m66 to unbound m66.6 identified three light-chain residues of m

143 y developed metadynamics-based protocol, the unbinding mechanism of an inhibitor of the pharmacologic

144 molecules in solution to participate in the unbinding mechanism.

145 e antibody-antigen pair ranking in bound and unbound models but recovers additional top-ranked cognat

146 ther method that can determine a fraction of unbound molecules at equilibrium.

147 hannels or pumps are needed for washing away unbound molecules.

148 rms current methods in determining bound and unbound motif sites.

149 tual features discriminate between bound and unbound motifs, we performed an in vitro binding assay u

150 The structure of unbound muPA revealed an unsuspected non-chymotrypsin-li

151 At heterotypic contacts with E-cadherin, unbound N-cadherin induces an asymmetric accumulation of

152 Plasma is a medium of unbound negative and positive particles with the overall

153 A hydrogen-bonding interaction with an unbound nitrogen atom of the naphthyridine-based ligand

154 mes a different conformation compared to the unbound NMR-based solution structure of the same PG-frag

155 Resurgent current results from unbinding of a blocking particle that competes with norm

156 m molecular dynamics (MD) simulations of the unbinding of a popular prototypical hydrophobic cavity-l

157 quires dephosphorylation of the receptor and unbinding of arrestin, processes that are poorly underst

158 ecule force spectroscopy (SMFS) to study the unbinding of Coh:Doc complexes under force.

159 are performed by considering the binding and unbinding of kinesins to microtubules and their dependen

160 Binding and unbinding of ligands changes its conformation that plays

161 potential involvement of TM5i in binding and unbinding of Na2, i.e. the Na(+) bound in the Na2 site,

162 at the rate parameters governing binding and unbinding of regulatory proteins to DNA strongly influen

163 rescence to observe directly the binding and unbinding of short oligonucleotides (7-12 nt) to a compl

164 The interplay between binding and unbinding of synaptic receptor proteins at synapses play

165 h our previous work suggest that binding and unbinding of the AD to the Bateman domain dimer induces

166 Herein, the FES of unbinding of the antagonist N-(3alpha-hydroxy-5beta-chol

167 aintaining interdomain coupling, retards the unbinding of the B-site cAMP and stalls an unproductive

168 ocessivity is determined by the race between unbinding of the bound head and attachment of the tether

169 Unbinding of the cAMPs appears ordered as indicated by a

170 which allowed us to measure the force due to unbinding of the colloidal probe and the planar membrane

171 The binding/unbinding of the human thrombin and its 15-mer single st

172 further unveil a sequence of events, whereby unbinding of the hydrolysis product (ADP + Pi) is follow

173 Diffusive search, recognition, binding, and unbinding of these proteins often amount to kinetic bott

174 Unbinding of TM5 from the rest of the structure exposes

175 Binding and unbinding of transcription regulators at operator sites

176 of collagen gels arises from force-dependent unbinding of weak bonds between collagen fibers.

177 tioning of cells (efficiency >/= 10(6)) from unbound oligonucleotides at high volume throughput (~2 x

178 A condensation that is reversible by protein unbinding or force.

179 ating the existence of a large population of unbound or wide-orbit Jupiter-mass planets (reported to

180 The unbound p110 protein is unstable, leading to the attenua

181 quantitatively correlated with the amount of unbound PAH molecules in the AuNP suspensions, as judged

182 rtially bound state allows the protein to be unbound, partially bound, or fully bound to a DNA strand

183 provide a thorough description of the ligand unbinding pathway identifying the most stable binding mo

184 ylated and phosphorylated ARH1, the possible unbinding pathways of ADP-ribose from non-phosphorylated

185 ics-based approach allows exploration of the unbinding pathways, estimation of the rates, and determi

186 We discover that while unbound PD-1 exhibits a hard-to-drug hydrophilic interfa

187 more than the number of currently available unbound peptide macrocycle structures.

188 tide digest of prolamins), the flow-through (unbound peptides), and the output (captured peptides) we

189 rance rates (relative to normal subjects) of unbound phenylacetylglutamine (0.37-fold), hippurate (0.

190 result in a major decrease in the number of unbound phosphorylated SH2 domain-binding sites.

191 Unbound piperacillin plasma concentration and fractional

192 Unbound planets can also be formed through gravitational

193 ues ( approximately 5 muM) approximating its unbound plasma concentration (determined by equilibrium

194 ng does not necessarily lead to high in vivo unbound plasma concentration.

195 Dsg2, is present in relevant amounts in the unbound pool in which activated p38 MAPK is predominantl

196 rt to the high tunability of their bound and unbound populations.

197 yrosines (Tys173 and Tys177) that in the CD4-unbound prefusion state mediate intramolecular interacti

198 ber: cargos driven by a few motors tended to unbind prematurely from the microtubule, whereas cargos

199 The unbinding probabilities corresponding to each mechanoche

200 robes are thermodynamically more stable than unbound probes, differential thermal analysis can remove

201 rent energies for the different steps in the unbinding process.

202 of the active site play crucial roles in the unbinding process.

203 tifying the rate-limiting step of the ligand unbinding process.

204 ferences in the MS spectra between bound and unbound protein allowed calculation of Kd values from th

205 A radical expansion of the unbound protein docking benchmark set was achieved by si

206 A large set of simulated unbound protein structures was generated from the bound

207 ads with subsequent proteolytic digestion of unbound proteins and peptide-based phosphorylation enric

208 xposed to 100mg of mAzo and washed to remove unbound proteins by magnetic field.

209 nd, alpha-helical state toward the inactive, unbound, random-coil state.

210 Anchors that bind and unbind rapidly from matrix accumulate on nanoparticulate

211 From our simulations, we estimated the unbinding rate as koff = 0.020 +/- 0.011 s(-1).

212 Binding and unbinding rate constants measured across a wide range of

213 The unbinding rate k off is computed from the mean residence

214 Specifically, the decrease in the unbinding rate of calcium to troponin C with increasing

215 binding rate of the inactive state and slow unbinding rate of the active state, for a higher effecti

216 nucleoid-associated proteins that exhibit an unbinding rate that depends on the concentration of free

217 rdered as indicated by a large difference in unbinding rates from the two sites, but the cause has re

218 ble dagger) of the force-dependent unfolding/unbinding rates is interpreted as the distance from the

219 cal method for estimating diffusion, binding/unbinding rates, and active transport velocities using F

220 as compared to the excited MLCT state of the unbound Re catalyst or when immobilized on ZrO2, which r

221 k also revealed a basal affinity of c-di-GMP-unbound receptor for LapG, the relevance of which remain

222 ul predictions were made for 21 bound and 18 unbound receptors.

223 to be highly flexible in simulations of the unbound RecU, in agreement with SAXS observations.

224 around the TF-bound sites to the counterpart unbound regions containing the same consensus motifs rev

225 f RNA secondary structure in the intervening unbound regions.

226 opy, were used to derive molecular models of unbound REs from pools of all-atom structures generated

227 eal sequence-dependent DNA shape features of unbound REs in solution.

228 tting the time courses of ligand binding and unbinding revealed modest cooperativity among the subuni

229 of crystal structures of EF-G bound and EF-G unbound ribosomal subunits.

230 ved placebo (14 participants) (P<0.001), and unbound rivaroxaban concentration was reduced by 23.4 ng

231 ous heavy meromyosin; the addition of excess unbound RLC, but not excess unbound essential light chai

232 Consequently, it is less clear how the unbound, sensing riboswitch refolds into the ligand bind

233 However, unbound soluble SAA is intrinsically disordered and is e

234 atic alpha-synuclein existed primarily in an unbound, soluble pool.

235 as in at least three different pools: (1) as unbound, soluble protein; (2) bound to presynaptic vesic

236 taneously concentrate and separate bound and unbound species in an assay based on electrical mobility

237 200 kilometers per second, it is the fastest unbound star in our Galaxy.

238 hydrins to act as molecular shields in their unbound state and as membrane protectants in their bound

239 The structural insights came from the unbound state and complexes of NagZ with the substrate,

240 ative to the initial current measured in the unbound state of antibody.

241 ing-in-frame a conformation distinct from an unbound state or anti complex.

242 oming clearer, how P-TEFb remains in the 7SK-unbound state to sustain transcription elongation progra

243 P1 conformation was studied in its bound and unbound state using mass spectrometry, small-angle X-ray

244 has its secondary structure preformed in the unbound state with only tertiary structure changes occur

245 ganded Env trimer or the gp41 peptide in the unbound state.

246 , with friction of the fragment matching the unbound state.

247 mains compared with their disposition in the unbound state.

248 open and closed states by FRET, and binding-unbinding states of the glycine ligand by anisotropy mea

249 tein structures determined in both bound and unbound states are essential for benchmarking of the doc

250 -have been thoroughly characterized, but the unbound states have been more elusive.

251 lar electrostatic interactions influence the unbound states of PUMA only marginally.

252 Bank structures of Bcl-xL in both bound and unbound states reveal substantial intrinsic heterogeneit

253 conversion between various protein bound and unbound states.

254 y interacting and completely noninteracting (unbound) states.

255 eeSRF) and show that, when quality bound and unbound structural data are available, FreeSRF correlate

256 The unbound structure shows a high level of disorder and an

257 al region (NTR) previously unseen in our DNA unbound structure.

258 al expansion of such sets is possible if the unbound structures are computationally simulated.

259 en complexes of which 11 have both bound and unbound structures available, and we generated a represe

260 in complex with its gp41 epitope, along with unbound structures of m66 and m66.6.

261 of protein-protein complexes along with the unbound structures of their components.

262 The simulated unbound structures were selected according to criteria f

263 rate of PLB for both ligand-bound and ligand-unbound structures, particularly for top choice predicti

264 rison of experimentally determined bound and unbound structures.

265 benchmark set was achieved by simulating the unbound structures.

266 Since unbound TALEs retain superhelical structure, it seems li

267 omes contain millions of randomly occurring, unbound TF consensus binding sites.

268 ormational changes in the process of binding/unbinding the transducer.

269 perpendicular to the pulling direction that unbinds the proteins and forms force-independent ideal b

270 tion of positive electric field successfully unbinds the thrombin from the aptamer.

271 sion increases faster than cross-linkers can unbind, the marginal band will coil, whereas if the tens

272 constrained to move in an axial manner, the unbinding time is found to be on the order of 4,000 s.

273 We validate the unbinding timescales from metadynamics through a Poisson

274 E220 in maintaining structural stability in unbound TIS11d and elucidated the underlying physical me

275 the C-terminal residues in the structure of unbound TIS11d, the E220A mutant and the truncation muta

276 lves the coupling of microtubule binding and unbinding to a change in the configuration of the linker

277 teins diffusing while stochastically binding/unbinding to various affinity sites in living cells give

278 K(+) and Na(+), mainly unbound to any ligand in the sample, have been identifie

279 y direct photoexcitation of substrates while unbound to catalyst.

280 ive chemistry program increased the fraction unbound to human plasma protein from below minimum detec

281 n that was exploited to improve the fraction unbound to human plasma protein while retaining biochemi

282 Plasma concentrations of protein unbound, total RAL, and RAL glucuronide were determined

283 Unbound tracer underwent rapid renal clearance followed

284 We uncover the complex pathways of unbinding trajectories and describe the critical rate-li

285 Using metadynamics, multiple unbinding trajectories that start with the ligand in the

286 s per second, suggesting highly eccentric or unbound trajectories if they are associated with physica

287 Beyond the unbinding transition of the lamellar phase, extremely as

288 The binding and unbinding transitions of Vn at the membrane interface ar

289 We were able to reproduce the unbinding trends seen experimentally for both DNA and pr

290 teractions that are otherwise present in the unbound, unfolded conformation of hTRF1.

291 UbiX: an FMN-bound wild type form and an FMN-unbound V47S mutant form.

292 he local concentration of tPA through forced unbinding via degradation of fibrin and tPA release.

293 ch greater absolute values than those of the unbound virions or antibodies while typically complexes

294 Estimation of unbound vitamin A in milk protein-Vit A complexes was ca

295 These complexes were further evaluated for unbound vitamin A, ability of milk protein to bind vitam

296 n-water interactions, and the most mobile to unbound water.

297 ed on the observation of streptavidin-biotin unbinding, we also conclude that the magnitude of integr

298 ure forces comparable to biotin:streptavidin unbinding were observed.

299 ll poles through rapid cycles of binding and unbinding within the PopZ scaffold.

300 convertase and C3 convertase, as well as the unbound zymogen C2 obtained by small angle x-ray scatter