ライフサイエンスコーパス: unbinding

1 ents but with slower kinetics of binding and unbinding.

2 ard tail currents are elicited upon spermine unbinding.

3 are based on the rates of ligand binding and unbinding.

4 consistent with slower glutamate binding and unbinding.

5 attachment affected the rates of binding and unbinding.

6 n, plastic deformation is observed only with unbinding.

7 e potentials of mean force for acetylcholine unbinding.

8 binding to the 70S ribosome, rather than its unbinding.

9 by increasing the probability that following unbinding, a ligand will rebind as opposed to being lost

10 However, the extent of DNA unbinding after addition of negatively charged membrane

11 The unbinding along the fibril axis largely depends on the i

12 Lateral mechanical unbinding and the unbinding along the fibril axis load different types of

13 gimes: an adiabatic regime with fast binding/unbinding and a nonadiabatic regime with slow binding/un

14 with bulk solvent affect ligand binding and unbinding and also the catalytic properties.

15 requires local remodeling through crosslink unbinding and and/or filament disassembly.

16 rated fractures and self-healing, as well as unbinding and annihilation of oppositely charged disclin

17 analysis of the model reveals that binding, unbinding and internalisation rates, the fraction of rec

18 ng that roadblock circumvention involves the unbinding and rebinding of the motors.

19 rk elasticity and force-induced cross-linker unbinding and rebinding.

20 AChEs within the synapse by using sequential unbinding and relabeling with different colors of strept

21 both allosteric and direct catalysis of GDP unbinding and release and GTP binding.

22 al that these effects are due to slowed GABA unbinding and slowed recovery from desensitization.

23 nd to be brittle in the lateral direction of unbinding and soft along the fibril axis.

24 The coupled motion between PRFAR unbinding and the directed opening of the interface is i

25 Lateral mechanical unbinding and the unbinding along the fibril axis load d

26 ver, the mechanism and pathway(s) of CRN/GBB unbinding and translocation, or even the ability of the

27 model to compare the relative importance of unbinding and unfolding of actin cross-linking proteins

28 Intriguingly, further high-temperature unbinding and unfolding simulations in implicit and expl

29 state to the transition state for unfolding/unbinding and, hence, often related to the mechanical co

30 escence recovery after photobleaching, photo-unbinding, and quantitative fluorescence imaging to inve

31 The kinetics of binding and unbinding are evaluated by intermittent imaging and coun

32 ormational states and the nucleotide binding/unbinding are mainly thermally driven.

33 easure transition rates among states and for unbinding as a function of buffer ionic strength.

34 he model predicts a delayed inertial protein unbinding associated with the SNARE complex assembly imm

35 etics of fibril growth is reconstructed from unbinding assuming the reversibility of deposition/disso

36 Mg(2+) unbinding at the divalent cation sensor triggers a confo

37 In agreement with experiment, the SMD unbinding barrier for SQ(-) is larger than for UQ.

38 The rate constants for binding and unbinding based on Piezo1 current kinetics provided asso

39 ominantly by an alternate pathway, where the unbinding becomes 20 times faster, and the sharp wetting

40 e transport with fingerprints of a nanoscale unbinding/binding behavior.

41 reaction coordinate shows that disaccharide unbinding/binding presents no energetic barrier and, the

42 ill not only shed first light on the role of unbinding but will also allow one to determine the time

43 shows significant peptide unfolding and some unbinding, but no insertion.

44 ck loop with time delays due to slow binding/unbinding can also generate oscillations.

45 form and it turns out that the work of their unbinding can be larger than the curvature energy reduct

46 mulations demonstrate that dimer binding and unbinding can occur early in the folding process before

47 A transition in which the effect of unbinding changes from inhibitory to excitatory as subst

48 Significantly, Na(+) binding (and unbinding) consistently involves a transient binding to

49 Feature unbinding contributed significantly to sequential effect

50 ental and theoretical approach named binding-unbinding correlation spectroscopy (BUCS), we describe t

51 Moreover, after unbinding, CTCF quickly rebinds another cognate site unl

52 Analysis of the unbinding data revealed a most probable interaction forc

53 Single-molecule forced unbinding demonstrated KKO was 8-fold more reactive towa

54 rane can also be determined and the rates of unbinding determined from a histogram of residence times

55 e that this correction enables prediction of unbinding distances and dissociation rates that are deco

56 for sequence homology by rapidly binding and unbinding double-stranded DNA (dsDNA) until homology is

57 ional changes that accompany ion binding and unbinding during the transport cycle.

58 study paves the way to investigations on the unbinding dynamics of more complex p38 inhibitors and ot

59 lowly relax towards equilibrium owing to the unbinding dynamics of the crosslinking molecules.

60 blies to reveal and characterize binding and unbinding dynamics within such assemblies.

61 f complex behaviors, possibly due to binding/unbinding dynamics, were observed.

62 Dynamic control of LH binding/unbinding, either globally or locally, in the presence o

63 tical model that incorporates enzyme binding/unbinding, electron transport, and enzyme diffusion alon

64 Allowing different unbinding energies not only has equilibrium effects on t

65 lapse to a universal curve regardless of the unbinding energies used or the identity of the dissociat

66 heir potentials of mean force and calculated unbinding energies.

67 e of a noncurvature energy contribution, the unbinding energy of the L(alpha) phase bilayers, g(u), t

68 The unbinding energy per bond was found to be on the order o

69 ed that the Pr segment exhibits a binding<-->unbinding equilibrium.

70 hibits slow dynamics that fit with a binding-unbinding equilibrium.

71 site, but not Na2, and, consistently, sodium unbinding/escape to the extracellular (EC) region first

72 were obtained by Poisson analysis of single unbinding event peaks, yielding values of -0.75 +/- 0.04

73 se timing sequence of stochastic binding and unbinding events allows one receptor to provide informat

74 The sequence of the unbinding events and the maximum force required to separ

75 physiological and experimental strain rates, unbinding events are predominant with negligible unfoldi

76 tochastic process of innumerable binding and unbinding events between antibodies and the multiple bin

77 nitor individual ligand:receptor binding and unbinding events in space and time by single-molecule im

78 We showed that the collective frequency of unbinding events in WT-RBCs is not significantly differe

79 Single unbinding events observed at higher surface delays were

80 Further, the observation of sequential unbinding events strongly suggests a multivalent binding

81 that we can follow the series of binding and unbinding events that lead to the onset of active transp

82 Explore is able to observe millisecond-scale unbinding events using many nanosecond-scale trajectorie

83 s showed that the mean force of the specific unbinding events was 32 +/- 5 pN, and the hydrodynamic d

84 ite the highly dynamic nature of binding and unbinding events, under fast loading rates relevant to T

85 Unbinding follows the inverse sequence of events with th

86 tle is known on how cells can modulate their unbinding for regulation.

87 lysis the consequences of such force-induced unbinding for T cell recognition.

88 proteins on the RBC surface and measures the unbinding force between BCAM/Lu and LAMA5.

89 and SS-RBC membranes, as well as the median unbinding force between ICAM-4 and alphavbeta3.

90 However, the unbinding force between ICAM-4 and the corresponding lig

91 Additionally, at least 20% variation in unbinding force can be attributed to minute differences

92 In four cases we observed bimodal unbinding force distributions, indicating conformational

93 dissociation events, disparities in measured unbinding force F(R) among these methods lead to marked

94 The maximum unbinding force for this interaction was approximately 1

95 erturb the energy landscape and the apparent unbinding force of the complex for sufficiently stiff fo

96 licitly includes the effect of k on apparent unbinding force of the ligand-receptor complex, and demo

97 ression of active BCAM/Lu receptors, a lower unbinding force to LAMA5, and insignificant stimulation

98 on cells, as measured by an increase in the unbinding force.

99 We show that the measured individual unbinding forces between ezrin and F-actin are independe

100 Unbinding forces between ligands and SGLT1 were recorded

101 g on the direction of force application, the unbinding forces can more than triple.

102 nd formation and dissociation under constant unbinding forces, mimicking the forces of physiologic bl

103 merize, and that the dynamics of binding and unbinding from beta-catenin, possibly coupled with lower

104 of current typically associated with blocker unbinding from ion channels.

105 labeling of complexin can modify its rate of unbinding from SNAREs.

106 ool of endophilin at synapses is provided by unbinding from the adjacent SV pool and that the unbindi

107 he allosteric site sterically hinders ligand unbinding from the central site, providing an explanatio

108 nce of structural events that couple agonist unbinding from the extracellular domain to ion-pore clos

109 lows the kinetics of calcium removal and IP3 unbinding from the receptor and is not limited by IP3 me

110 , and it reverses within seconds of arrestin unbinding from the transient binding mode.

111 remove the steric constraints on ligand, the unbinding happens predominantly by an alternate pathway,

112 rium steered MD simulations of the inhibitor unbinding have also been performed.

113 bited Vn to supported lipid bilayers, and to unbinding in freestanding lipid vesicles.

114 role of dynamic-linker histone (LH) binding/unbinding in high monovalent salt with divalent ions, an

115 d networks, a finding that also accounts for unbinding in structurally unrelated integrin-fibronectin

116 62 sidechain, which mimic sodium binding and unbinding in the Na(+)-coupled substrate symporters.

117 A generic mechanism of dislocation unbinding in the presence of varying Gaussian curvature

118 cules (such as switching between binding and unbinding) in the in vivo environment of living cells.

119 ta(2)R207A and alpha(1)R132A sped antagonist unbinding, indicating that these arginines stabilize the

120 ace, we identify four distinct categories of unbinding: inhibitory, excitatory, superexcitatory, and

121 ) and this means that the time course of its unbinding is comparable to the expected time course of t

122 FkappaB is the strongest DNA binder, and its unbinding is controlled kinetically by molecular strippi

123 sis for the energetics of ligand binding and unbinding is critical to our understanding of the pharma

124 Concentration-dependent unbinding is generated by this simple model, quantitativ

125 associated rate constants of protein-ligand unbinding is of great practical importance in drug desig

126 Lateral unbinding is primarily determined by the cooperative rup

127 s are slip-resistant such that force-induced unbinding is suppressed, which occurs in many biological

128 ent that is consistent with rapid Mg(o)(2+) -unbinding kinetics and also a slower, millisecond time s

129 A combination of the unbinding kinetics and the equilibrium constant allows t

130 Experimental approaches to binding and unbinding kinetics are nowadays available, but we still

131 at they show unusual concentration-dependent unbinding kinetics from chromosomal recognition sites in

132 ision single-molecule tracking, we study the unbinding kinetics from DNA of two metal-sensing transcr

133 Unexpectedly, their unbinding kinetics further varies with the extent of chr

134 Binding and unbinding kinetics monitored by fluorescence were slow.

135 ociation pathway and visualizing the binding-unbinding kinetics of a single DNA molecule.

136 in parallel, characterizing force-dependent unbinding kinetics of an antibody-antigen pair in minute

137 trap to probe the velocity, run length, and unbinding kinetics of mouse KIF3A/B under various loads

138 In a regime of slow DNA-binding/unbinding kinetics, spontaneous switching occurs relativ

139 In contrast, in a regime of fast DNA-binding/unbinding kinetics, switching occurs rarely and is drive

140 Besides quantifying its DNA binding and unbinding kinetics, we discovered that CueR spontaneousl

141 mab, inhibited binding without affecting the unbinding kinetics, whereas Mn(2)(+) biased the alphaIIb

142 tion initiation; it modulates RNAP's binding-unbinding kinetics, without allowing interconversions be

143 designing new drugs with engineered binding/unbinding kinetics.

144 lar noise due to capping protein binding and unbinding leads to macroscopic filopodial length fluctua

145 Here we show that unbinding may also speed up enzymatic turnover--turning

146 dition, partial sensor occupancy due to slow unbinding may contribute to the linearization of the fir

147 the photoreceptor Ca(2+) sensor, slow Ca(2+) unbinding may support the fusion of vesicles located at

148 between cationic and anionic lipids) and DNA unbinding (measured as accessibility of DNA to ethidium

149 y developed metadynamics-based protocol, the unbinding mechanism of an inhibitor of the pharmacologic

150 molecules in solution to participate in the unbinding mechanism.

151 In response to agonist binding and unbinding, members of this superfamily undergo a series

152 the CaM N-terminal domain, we reveal binding-unbinding motions of the N-terminal domain of the CaM in

153 10 nM) to very low (>100 muM) by binding and unbinding MTSET.

154 nding increases the dsDNA tension, then RecA unbinding must decrease tension.

155 for the practical range of behavior in which unbinding occurs diffusively rather than ballistically,

156 chain that will hold the domains together if unbinding occurs.

157 Resurgent current results from unbinding of a blocking particle that competes with norm

158 m molecular dynamics (MD) simulations of the unbinding of a popular prototypical hydrophobic cavity-l

159 me, repeated measurements of the binding and unbinding of a receptor and ligand in a single molecule

160 The threshold forces associated with lateral unbinding of Abeta peptides exceed those observed during

161 lecular dynamics, we study the force-induced unbinding of Abeta peptides from the fibril.

162 To investigate the kinetics of unbinding of Abeta(16-22) monomers from preformed fibril

163 t that despite the possibility of unfolding, unbinding of ACPs is the major determinant for the rheol

164 quires dephosphorylation of the receptor and unbinding of arrestin, processes that are poorly underst

165 ecule force spectroscopy (SMFS) to study the unbinding of Coh:Doc complexes under force.

166 Forced unbinding of complementary macromolecules such as ligand

167 motes rapid recognition by driving the swift unbinding of dsDNA from non-homologous Rad51-ssDNA filam

168 be visualized where dynamics of binding and unbinding of fluorescent protein complexes occur.

169 , namely, the binding of GTP-bound monomers, unbinding of GTP- and GDP-bound monomers, and hydrolysis

170 croscopy was used to observe the binding and unbinding of hapten decorated quantum dots to individual

171 The binding and unbinding of individual tetramethylrhodamine-labeled neu

172 surements showed that the force required for unbinding of integrin-ligand interactions increases over

173 are performed by considering the binding and unbinding of kinesins to microtubules and their dependen

174 Binding and unbinding of ligands changes its conformation that plays

175 eractions predict that transient binding and unbinding of multiple tethers to each synaptic vesicle m

176 potential involvement of TM5i in binding and unbinding of Na2, i.e. the Na(+) bound in the Na2 site,

177 teered molecular dynamics (SMD) to model the unbinding of neutral ground state ubiquinone (UQ) and it

178 on-Crick pairing partners promotes the rapid unbinding of non-homologous dsDNA and drives strand exch

179 que role of shape in determining binding and unbinding of particles to cell surface.

180 e profile of potential of mean force for the unbinding of PIIIA from the channel, and predict that PI

181 ion: the fluctuation due to the slow binding/unbinding of protein regulators to gene promoters.

182 at the rate parameters governing binding and unbinding of regulatory proteins to DNA strongly influen

183 ated states can emerge from the slow binding/unbinding of regulatory proteins to gene promoters.

184 rescence to observe directly the binding and unbinding of short oligonucleotides (7-12 nt) to a compl

185 an affinity dependent manner, as the binding/unbinding of such receptor or ligand molecules crucially

186 The interplay between binding and unbinding of synaptic receptor proteins at synapses play

187 h our previous work suggest that binding and unbinding of the AD to the Bateman domain dimer induces

188 Herein, the FES of unbinding of the antagonist N-(3alpha-hydroxy-5beta-chol

189 aintaining interdomain coupling, retards the unbinding of the B-site cAMP and stalls an unproductive

190 ocessivity is determined by the race between unbinding of the bound head and attachment of the tether

191 Unbinding of the cAMPs appears ordered as indicated by a

192 Receptor ligation thus needs to result in unbinding of the CD3epsilon ITAM from the membrane to re

193 which allowed us to measure the force due to unbinding of the colloidal probe and the planar membrane

194 The binding/unbinding of the human thrombin and its 15-mer single st

195 further unveil a sequence of events, whereby unbinding of the hydrolysis product (ADP + Pi) is follow

196 n the activation energy, suggesting that the unbinding of the ligand occurs after the transition stat

197 investigate the mechanism of the binding and unbinding of the molecules.

198 Diffusive search, recognition, binding, and unbinding of these proteins often amount to kinetic bott

199 Unbinding of TM5 from the rest of the structure exposes

200 Binding and unbinding of transcription regulators at operator sites

201 of collagen gels arises from force-dependent unbinding of weak bonds between collagen fibers.

202 blish the dependence of force history during unbinding on both those parameters chosen to characteriz

203 as shown by control experiments), results in unbinding on millisecond timescales, giving rise to rapi

204 n, stress relaxes to physiological levels by unbinding only--not unfolding--of ACPs, which is consist

205 A condensation that is reversible by protein unbinding or force.

206 Interestingly, the unbinding pathway from the primary site appears to be di

207 provide a thorough description of the ligand unbinding pathway identifying the most stable binding mo

208 ylated and phosphorylated ARH1, the possible unbinding pathways of ADP-ribose from non-phosphorylated

209 lar dynamics simulations reveal a variety of unbinding pathways that indicate a highly dynamic intera

210 ics-based approach allows exploration of the unbinding pathways, estimation of the rates, and determi

211 From multiple trajectories along these unbinding pathways, we calculated the potentials of mean

212 several intermediate states exist along the unbinding pathways.

213 sults indicate that the interactions between unbinding peptides constitute the molecular basis for co

214 For E. coli, we find that the binding/unbinding phase boundary is hyperbolic.

215 Analytically constructing the unbinding phase space, we identify four distinct categor

216 The unbinding probabilities corresponding to each mechanoche

217 ion and provide a picture of the binding and unbinding process in which dim molecules attach to the a

218 ations, we provide a detailed picture of the unbinding process of three clinically relevant GR modula

219 rent energies for the different steps in the unbinding process.

220 of the active site play crucial roles in the unbinding process.

221 and residues in the binding site during the unbinding process.

222 tifying the rate-limiting step of the ligand unbinding process.

223 ds on the time scale of the promoter binding/unbinding processes.

224 smon resonance measurements show a very slow unbinding rate (1/115 min) for inhibitor 7m.

225 SR-95531 has a fast unbinding rate (k(offSR), about 3000 s(-1)) and this mea

226 From our simulations, we estimated the unbinding rate as koff = 0.020 +/- 0.011 s(-1).

227 es per receptor with microscopic binding and unbinding rate constants for physostigmine of 20 microM(

228 Binding and unbinding rate constants measured across a wide range of

229 sides of the membrane and the corresponding unbinding rate constants.

230 nding from the adjacent SV pool and that the unbinding rate is regulated by exocytosis.

231 The unbinding rate k off is computed from the mean residence

232 Specifically, the decrease in the unbinding rate of calcium to troponin C with increasing

233 The fast unbinding rate of SR-95531 from the glycine receptor wil

234 binding rate of the inactive state and slow unbinding rate of the active state, for a higher effecti

235 ion of values of the nonspecific binding and unbinding rate parameters that balance the protein trans

236 efficients for given nonspecific binding and unbinding rate parameters.

237 nucleoid-associated proteins that exhibit an unbinding rate that depends on the concentration of free

238 uned in a wide range by changing the binding/unbinding rate without changing the amplitude much, whic

239 evenfold slower than the microscopic subunit unbinding rate.

240 ed the effects on microscopic ligand binding/unbinding rates and channel gating.

241 Based on force-dependent unbinding rates and pilus retraction speeds measured at

242 tions demonstrate that slow GABA binding and unbinding rates could reproduce the characteristic long-

243 rdered as indicated by a large difference in unbinding rates from the two sites, but the cause has re

244 pic and macroscopic (or local and non-local) unbinding rates have been previously proposed, but never

245 ble dagger) of the force-dependent unfolding/unbinding rates is interpreted as the distance from the

246 d concentration jumps to measure binding and unbinding rates of a high-affinity aspartate analog that

247 ur is explained by the different binding and unbinding rates of both integrin types to fibronectin.

248 ork has demonstrated concentration-dependent unbinding rates of proteins from DNA, using fluorescence

249 s and the dependence of the scaffold binding/unbinding rates on the number of phosphorylated sites.

250 cal method for estimating diffusion, binding/unbinding rates, and active transport velocities using F

251 eling, we further demonstrated that the GABA unbinding rates, in particular, are strongly coupled.

252 ity sequences determined for the binding and unbinding rates, which are negative-negative-positive an

253 tic turnover under a controlled variation of unbinding rates.

254 differentiation for certain promoter binding/unbinding rates.

255 re optimization of drug-receptor binding and unbinding rates.

256 g rate, and accelerate deactivation and GABA unbinding rates.

257 ropic random-walk model coupled with binding-unbinding reactions that weaken the fiber.

258 nd) measurements of dye-membrane binding and unbinding reactions, for all members of this family of p

259 de that they are generated by proton-binding/unbinding reactions.

260 pical in vivo conditions, dynamic-LH binding/unbinding reduces fiber stiffening dramatically (by a fa

261 and a nonadiabatic regime with slow binding/unbinding relative to protein synthesis/degradation.

262 g of the determinants of the rates of phenol unbinding remains obscure, chiefly because residues impl

263 Thus, the voltage dependence of blocker unbinding results almost entirely from repulsion by Na i

264 tting the time courses of ligand binding and unbinding revealed modest cooperativity among the subuni

265 ed by NMR are not all associated with likely unbinding routes suggested by the best-resolved hexamer

266 remaining trapped in the pore during agonist unbinding showed the strongest dependence on extracellul

267 open and closed states by FRET, and binding-unbinding states of the glycine ligand by anisotropy mea

268 that bound MDM2 unfolds in the order of p53 unbinding, tertiary unfolding, and finally secondary str

269 tics of alphaIIbbeta3-fibrinogen binding and unbinding that underlie the dynamics of platelet adhesio

270 ormational changes in the process of binding/unbinding the transducer.

271 rder mechanism and suggesting that following unbinding, there is a significant probability of ligand

272 t strength is characterized by measuring the unbinding time as a function of applied force.

273 constrained to move in an axial manner, the unbinding time is found to be on the order of 4,000 s.

274 We validate the unbinding timescales from metadynamics through a Poisson

275 lves the coupling of microtubule binding and unbinding to a change in the configuration of the linker

276 We use a two-state model of RecA binding/unbinding to extract the associated thermodynamic parame

277 ime; and the time delays due to slow binding/unbinding to promoters in the nonadiabatic regime, which

278 se source connected with the protein binding/unbinding to the DNA.

279 teins diffusing while stochastically binding/unbinding to various affinity sites in living cells give

280 We uncover the complex pathways of unbinding trajectories and describe the critical rate-li

281 Using metadynamics, multiple unbinding trajectories that start with the ligand in the

282 these disparities and examined atomic-level unbinding trajectories via steered molecular dynamics si

283 Beyond the unbinding transition of the lamellar phase, extremely as

284 The binding and unbinding transitions of Vn at the membrane interface ar

285 We were able to reproduce the unbinding trends seen experimentally for both DNA and pr

286 its constitutive strands causes rapid dsDNA unbinding unless sufficient homology is present.

287 n hexamer dissolution timescales, and phenol unbinding upon dilution is likely the first step in the

288 whose rapid binding upon depolarization and unbinding upon repolarization minimizes fast and slow in

289 he local concentration of tPA through forced unbinding via degradation of fibrin and tPA release.

290 However, during channel deactivation, ligand unbinding was slower than channel closing, suggesting a

291 entually becomes infinite at full hydration (unbinding); we attribute this both to electrostatic repu

292 ed on the observation of streptavidin-biotin unbinding, we also conclude that the magnitude of integr

293 With slow promoter binding/unbinding, we found multiple meta-stable differentiated

294 ure forces comparable to biotin:streptavidin unbinding were observed.

295 However, the off-rates for Ca(2+) unbinding were unexpectedly slow.

296 as seen to stabilise the DSE product against unbinding, which also proceeded in the simulations by a

297 which any increase in the rate of substrate unbinding will decrease the rate of enzymatic turnover.

298 beta(2)R207A slowed agonist binding and sped unbinding with little effect on gating, demonstrating th

299 ll poles through rapid cycles of binding and unbinding within the PopZ scaffold.

300 Third, load-induced unbinding yields a reduced coefficient of variation of t