ライフサイエンスコーパス: uncage

1 S (laser-scanning photostimulation/glutamate uncaging).

2 ough voltage-gated Ca(2+)-channels or Ca(2+) uncaging.

3 cells using two-photon imaging and glutamate uncaging.

4 yquinoline (BHQ)-based linker for two-photon uncaging.

5 py, in combination with two-photon glutamate uncaging.

6 p to 100 ms before or up to 500 ms after IP3 uncaging.

7 s for convenient quantitative calibration of uncaging.

8 ng studies, biochemical analysis and InsP(3) uncaging.

9 +/- 7.5 % (n = 9) decrease in CBF following uncaging.

10 scanning microscopy and two-photon glutamate uncaging.

11 e a greatly reduced calcium release upon Sph uncaging.

12 atterned stimulation by two-photon glutamate uncaging.

13 tude decrease in metal binding affinity upon uncaging.

14 diminish fluorescence of the VF dye prior to uncaging.

15 tropic and ionotropic receptors or direct UV-uncaging.

16 aser-scanning photostimulation and glutamate uncaging.

17 sed and continuous patterns into the flow by uncaging a fluorescent dye, highly detailed measurements

18 s, so that chromophores with high two-photon uncaging action cross-sections (delta(u)) can be designe

19 IP3 uncaging also triggers oscillatory Ca(2+) release, but,

20 Here we use glutamate uncaging and a novel optogenetic strategy to track chang

21 ircuit mapping using 2-photon (2P) glutamate uncaging and analyze experience-dependent neonatal devel

22 nical aspects of performing neurotransmitter uncaging and channelrhodopsin-assisted circuit mapping,

23 Here we have used two-photon glutamate uncaging and compartmental modeling to reveal a gradient

24 Using two-photon glutamate uncaging and dendritic patch-clamp recordings, we found

25 near the channels, in response to glutamate uncaging and depolarization, respectively.

26 cellular resolution, using two-photon Ca(2+) uncaging and dynamic-clamp.

27 ly, NPE-HCCC2 and HCCC2 have high two-photon uncaging and excitation efficiency, respectively, enabli

28 f electrophysiological, two-photon glutamate uncaging and imaging methods, we show that mature indivi

29 fficiency, respectively, enabling two-photon uncaging and imaging to be combined to examine cell coup

30 NPs as probes for controlled localization of uncaging and imaging with multiphoton z-axis sectioning.

31 ted with MeCP2 deficiency, we used glutamate uncaging and laser scanning photostimulation to survey i

32 Here, we established presynaptic Sr(2+) uncaging and made quantitative Sr(2+)- and Ca(2+)-imagin

33 synaptically connected cell pairs, NPEC-AMPA uncaging and miniature current recordings.

34 nzyme activity (host-guest interactions with uncaging and molecular cleavage).

35 cell recordings, two-photon microscopy, GABA uncaging and optogenetics to study dendritic inhibition

36 ts (ICa(V)) with UV-light flash-induced Ca2+ uncaging and presynaptic Ca2+ imaging to study the Ca2+

37 Using two-photon glutamate uncaging and time-lapse imaging of rat hippocampal CA1 n

38 Here, we use two-photon glutamate uncaging and time-lapse imaging to show that non-ionotro

39 d presynaptic activity (two-photon glutamate uncaging and two-photon imaging of the FM 1-43 assay, re

40 Using glutamate uncaging and two-photon imaging, we demonstrate that the

41 ser scanning microscopy with glutamate photo-uncaging and whole-cell recording to examine synaptic st

42 ent probes, most of which rely on the photo-"uncaging" and photoisomerization reactions.

43 otoreactive groups to allow photoliberation (uncaging) and photo-cross-linking in a sequential manner

44 ocytes released glutamate in response to IP3 uncaging, and (2) glutamate released by astrocytes activ

45 cked the changes in sIPSCs induced by Ca(2+) uncaging, and bath application of a selective GluR5-cont

46 sing dendritic recording, 2-photon glutamate uncaging, and computational modeling, we investigated ho

47 siological, two-photon imaging and glutamate uncaging, and electron microscopic assays in acute brain

48 o stimulate presynaptic neurons by glutamate uncaging, and mapped the location of sites that provide

49 cell electrophysiology, two-photon glutamate uncaging, and optogenetics in TC slices containing the a

50 ing pharmacological manipulations, glutamate uncaging, and two-photon imaging of GFP-transfected hipp

51 the probability of triggering CICR by apical uncaging, and uncaging-induced CICR could activate long-

52 -photon laser scanning microscopy, glutamate uncaging, and whole-cell electrophysiological recordings

53 e the versatility of this general sequential uncaging approach by applying it to control Wip1 phospha

54 sed this question by applying the photolytic uncaging approach to induce focal increases in Ca2+ leve

55 a indicate that oscillations elicited by IP3 uncaging are driven by the biphasic regulation of the IP

56 (2+) wave velocity, whereas responses to IP3 uncaging are enhanced.

57 to steer an ultraviolet beam rapidly and can uncage at over 20,000 locations per second.

58 that allows wavelength-selective two-photon uncaging at 900 nm versus 720 nm.

59 endritic spines were stimulated by glutamate uncaging at a diffraction-limited spot, and the localiza

60 vesicle priming by using presynaptic Ca(2+) uncaging at a small, glutamatergic, central synapse.

61 ing combined with two-photon laser glutamate uncaging at apical spines of CA1 pyramidal neurons to ex

62 We used direct stimulation by calcium uncaging at identified, single-site inhibitory synapses

63 Two-photon (2p) glutamate uncaging at individual dendritic spines showed that E2 i

64 Using focal glutamate uncaging at individual synapses, we find only a subpopul

65 dal neurons in acute brain slices, glutamate uncaging at single spines showed that iGluSnFR responds

66 However, brief glutamate uncaging at spines on distal dendrites evoked somatic up

67 r Colo357 cells during light-controlled H(+) uncaging at the hypoxic core.

68 The uncaging beam is projected into the focal plane of a two

69 Importantly, prior to uncaging, bis-CNB-GABA is inactive at the GABAA receptor

70 se inhibitor vorinostat that was efficiently uncaged by heterogeneous Pd catalysis in cell culture mo

71 We used glutamate uncaging by laser scanning photostimulation to map the l

72 matic core that can be selectively reduced ("uncaged") by one cell type, liberating a luciferin that

73 a Nikon A1R confocal microscope, was used to uncage Ca(2)(+) or IP3 and conduct photobleaching experi

74 We found that uncaging Ca(2+) activated biphasic BK currents with fast

75 tracellular Ca(2+) in astrocytes, induced by uncaging Ca(2+), o-nitrophenyl-EGTA, increased action po

76 uffer exchange and a pulsed laser system for uncaging caged compounds.

77 aged cyclic RGD peptide and demonstrate that uncaging can be efficiently performed with biologically

78 The uncaging can be initiated via either a one- or two-photo

79 Uncaging caused Ca2+ levels to increase not only in the

80 Postphotolysis, uncaged copper promotes hydroxyl radical formation under

81 cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notc

82 Remarkably, the uncaging cross section of a 1-(2-nitrophenyl)ethyl (NPE)

83 We have also measured the two-photon uncaging cross sections of NPE-caged coumarins 2a and 5

84 rties of BODIPY dyes lead to photocages with uncaging cross sections over 10000 M(-1) cm(-1), values

85 scence contrast enhancement, remarkably high uncaging cross sections, noninvasive cellular delivery,

86 ed photolysis" may yield other cages of high uncaging cross sections.

87 hanistic study, together with the two-photon uncaging data, suggested that the coumarin moiety serves

88 Furthermore, multisite two-photon glutamate uncaging demonstrated that HCN channels control the ampl

89 ulation or to approximately 300 nM by Ca(2+) uncaging dilated parenchymal arterioles in control brain

90 g GABA synthesis via intracellular glutamate uncaging dramatically potentiated GABA release within 1

91 We examined the dispersion of the uncaged dye in the plastic microchannels and compared it

92 nsity, as expected for a chemical two-photon uncaging effect.

93 a, although their heads are activated by the uncaging event, as determined with calcium imaging.

94 ectively on spines and act locally to dampen uncaging-evoked Ca transients and somatic potentials.

95 es showed that E2 increases the amplitude of uncaging-evoked EPSCs (2pEPSCs) and calcium transients (

96 ignificantly increased the amplitude of both uncaging-evoked EPSPs (uEPSPs) and spine Ca transients.

97 stimulate individual spines while monitoring uncaging-evoked excitatory postsynaptic potentials (uEPS

98 Uncaging-evoked excitatory postsynaptic potentials and C

99 branches while simultaneously recording the uncaging-evoked excitatory postsynaptic potentials and l

100 Uncaging-evoked NMDA-R current amplitudes were independe

101 Uncaging-evoked potential amplitudes correlated inversel

102 hese findings, we also show rapid, glutamate-uncaging-evoked, time-locked BDNF release from single de

103 Data from glutamate-uncaging experiments and simulations indicate that mGlu2

104 Single spine glutamate-uncaging experiments confirmed that less than half of th

105 e utility of NP-EGTA and DMn in rapid Ca(2+)-uncaging experiments in the presence of Mg(2+).

106 Our intrapipette uncaging experiments provide definitive evidence for lon

107 Gradual intracellular Ca(2+) uncaging experiments revealed that exocytosis had a simi

108 Multiphoton glutamate uncaging experiments revealed that the increase in dendr

109 Multiphoton glutamate uncaging experiments revealed that the increase in dendr

110 Two-photon glutamate uncaging experiments show somatic depolarization enhance

111 a computational model validated by glutamate uncaging experiments.

112 Using Ca(2+) imaging, glutamate uncaging, fluorescence recovery after photobleaching and

113 Photolytic H(+) uncaging from 2-nitrobenzaldehyde also raised [Ca(2+)]i,

114 treatment with FC led to potentiation of the uncaged GABA response, suggesting nucleus-specific roles

115 decline in the response of Purkinje cells to uncaged glutamate that accounted for both the time cours

116 uR5 can signal from intracellular membranes, uncaging glutamate on a CA1 dendrite led to a local Ca(2

117 Uncaging glutamate over whole-cell patch-clamped cells i

118 We activated inputs at targeted locations by uncaging glutamate sequentially to generate apparent mot

119 Real-time Ca(2+) imaging and Ca(2+) uncaging here reveal that CDF turns out to be strikingly

120 Synaptic mapping by glutamate uncaging identified layer 2/3 as the main source of loca

121 However, results using glutamate uncaging implicated Ca(2+) influx through SNX-482-sensit

122 OT can be activated in neuron cell bodies or uncaged in dendrites to enable structural tracing via "b

123 oked by inositol-1,4,5-trisphosphate (InsP3) uncaging in airway SMCs.

124 e we describe a new optical system for rapid uncaging in arbitrary patterns to emulate complex neural

125 synaptic terminals, and two-photon glutamate uncaging in dendritic spines performed in brain slices t

126 ty in normoxic conditions and small molecule uncaging in hypoxia.

127 rotecting group for both one- and two-photon uncaging in living cells.

128 responses evoked with focal glutamate photo-uncaging in the presence of TTX.

129 synaptic transmission and responses to GABA uncaging in the thalamic reticular nucleus (nRT) that is

130 macological stimuli or cell-specific calcium uncaging in vascular smooth muscle cells or astrocytes.

131 perturb Ca(2+) oscillations elicited by IP3 uncaging, indicating that reloading of endoplasmic retic

132 included to block action potentials, Ca(2+) uncaging induced a small decrease in the frequency of mi

133 y of triggering CICR by apical uncaging, and uncaging-induced CICR could activate long-lasting Ca2+ o

134 ive effects on AMPAR recycling and glutamate uncaging-induced structural and functional plasticity.

135 Using localized uncaged InsP(3), the forward movement of the Ca(2+) wave

136 H(+) uncaging into buffer mixtures in vitro demonstrated that

137 neurons, but had no effect on AHPs evoked by uncaging intracellular Ca(2+) .

138 The mechanism of the photoinduced uncaging involves homolytic C-C bond fragmentation follo

139 Ca(2+) waves could also be evoked by uncaging IP(3) with a UV flash in the dendrites.

140 could be generated by Ca(2+) waves evoked by uncaging IP(3), showing that other signalling pathways a

141 confined to the main apical dendrite because uncaging IP3 in the oblique dendrites has no effect on t

142 -yl)-propyl-1-phosphonic acid and CNQX or by uncaging IP3.

143 the adjacent stratum radiatum astrocytes by uncaging IP3.

144 had no effect on Ca(2+) increases induced by uncaging IP3.

145 The final uncaging is accomplished within 32 mus.

146 pines in response to low-frequency glutamate uncaging is saturable, reversible, and requires NMDA rec

147 reduce short-term facilitation when GABA is uncaged just prior to the onset of stimulation.

148 ing CAPS-proteins, suggesting that PI(4,5)P2 uncaging may bypass CAPS-function.

149 calculations, we can characterize the entire uncaging mechanism and identify the most relevant interm

150 we used patch-clamp, imaging, and glutamate uncaging methods in rat olfactory bulb slices to test fo

151 r GABA in its natural state using two-photon uncaging microscopy for the first time.

152 he soma and dendritic tree, whereas the fast uncaging minimized the effects of desensitization of alp

153 ngs, elevation of intracellular Ca(2+) by UV uncaging of 1-(4,5-dimethoxy-2-nitrophenyl)-EDTA-potenti

154 On the other hand, two-photon uncaging of 4-methoxy-7-nitroindolinyl-caged L-glutamate

155 ventricles of live zebrafish by means of the uncaging of a fluorescein derivative.

156 small groups of cells in this area by laser uncaging of a fluorescent dextran, and then tracked thei

157 f techniques by demonstrating the mechanical uncaging of a reactive species within a single protein.

158 Photochemical uncaging of bio-active molecules was introduced in 1977,

159 Uncaging of bis-CNB-GABA evokes inward GABAergic current

160 Localized photolytic uncaging of Ca(2+) from o-nitrophenyl-EGTA in somatic ER

161 r NP-EGTA, the UV flash photolysis-catalysed uncaging of Ca(2+) generated CICR in only 9% of the beta

162 hondria were activated specifically by local uncaging of Ca(2+) in the nucleus.

163 c myocytes, where either one- and two-photon uncaging of Ca(2+) induced highly local or cell-wide phy

164 Here, we utilize the photo-uncaging of Ca(2+) with CaV 2.1 channels fluxing Li(+) c

165 nd FAK inhibits repulsive turning from focal uncaging of Ca(2+) within filopodia.

166 thereby allowing CICR to be generated by the uncaging of Ca2+ (UV flash photolysis).

167 Because the uncaging of Ca2+ fails to stimulate CICR in the absence

168 In the apical region, local uncaging of Ca2+ was able to trigger a CICR wave, which

169 is retraction was prevented locally by focal uncaging of caged Ca(2+) that triggered Ca(2+) release f

170 f intact pancreatic acinar cells using local uncaging of caged Ca2+.

171 y carbacyclin-stimulated elevations in cAMP, uncaging of cAMP or exposure to a high concentration of

172 photon active at 720 nm, optically selective uncaging of DEAC450-caged biomolecules at 900 nm may all

173 Two-photon uncaging of DEAC450-Glu at 900 nm at spine heads on pyra

174 The synthesis and uncaging of dendrimer- and polymeric bead-based model sy

175 Uncaging of DMn is considerably faster, but DMn has a si

176 ution using optically independent two-photon uncaging of each neurotransmitter.

177 exhibited direct (non-synaptic) responses to uncaging of excitatory and inhibitory transmitters.

178 Two-photon uncaging of fluorescein near nonsuperfused gastric surfa

179 Using uncaging of GABA, there is a decreasing GABAergic influe

180 his electrical filtering, we used two-photon uncaging of glutamate and compared the integration of el

181 photon imaging, optogenetics, and dual-color uncaging of glutamate and GABA, we demonstrate that plat

182 t is accomplished using UV laser-based photo-uncaging of glutamate and imaging neuronal activation by

183 Laser-scanning photo-uncaging of glutamate focally activated neurons in layer

184 d two recently developed methods, two-photon uncaging of glutamate for determining the EPSC of indivi

185 Moreover, activation of MOPP cells by focal uncaging of glutamate induced strong inhibition of granu

186 Using two-photon uncaging of glutamate on spine heads from mouse layer-5

187 ) on depolarizations generated by two-photon uncaging of glutamate on spines from mouse neocortical p

188 ut screening method exploiting laser-induced uncaging of glutamate to construct excitatory input maps

189 Here, we use two-photon laser uncaging of glutamate to directly activate glutamate rec

190 Focal uncaging of glutamate, accomplished by switching a pulse

191 hoton laser scanning microscopy and 2-photon uncaging of glutamate, Carter and Sabatini (this issue o

192 ation of EPSPs, generated through two-photon uncaging of glutamate, this action was largely shunted b

193 ser scanning microscopy and two-photon laser uncaging of glutamate, we show that SK channels regulate

194 ser-scanning microscopy and two-photon laser uncaging of glutamate.

195 g iontophoresis of l-aspartate or two-photon uncaging of glutamate.

196 Uncaging of glycerophosphoryl-myo-inositol 4,5-bisphosph

197 ereas postsynaptic stores (activated by spot-uncaging of inositol 1,4,5-trisphosphate) remain unaffec

198 ith UDP-GlcNAc had an attenuated response to uncaging of InsP3.

199 eover, the Ca(2+) responses to melatonin and uncaging of IP(3) were mutually exclusive in infected RB

200 Photo-uncaging of IP3 in neurons expressing PI3K* elicits a ma

201 ow-intensity tetanic synaptic stimulation or uncaging of IP3 increased the decay time of spontaneous

202 On uncaging of IP3, astrocyte Ca2+ responses reliably propa

203 Furthermore, uncaging of MNI glutamate reveals that thalamocortical n

204 Two-photon (2P) uncaging of MNI-glutamate onto individual spines suggest

205 ary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing.

206 Photolytic uncaging of p-hydroxyphenacyl (pHP) GABA demonstrates ba

207 of presynaptic GABA receptors by photolytic uncaging of RuBi-GABA has a biphasic effect on EPSC ampl

208 (520 mum) by excitability testing and focal uncaging of RuBi-GABA on the axon at varying distances f

209 le-cell patch-clamp recording and photolytic uncaging of RuBi-GABA.

210 nitrodibenzofuran (NDBF) for ultra-efficient uncaging of second messengers inside cells.

211 Thus, optical uncaging of signaling lipids can uncover their rapid eff

212 Further, uncaging of Sph leads to the translocation of the autoph

213 micropatterns by means of photolithographic uncaging of surface amines.

214 on as well as pinpoint the location at which uncaging of the molecules occurred.

215 aling lipids sphingosine and diacylglycerol; uncaging of the probe for these two species triggered ca

216 In addition, through uncaging of the same compound, gene expression is contro

217 a rapid decrease in pH that accompanies the "uncaging" of an effector molecule from o-nitrobenzaldehy

218 e, simulation of cortical input by glutamate uncaging on proximal dendritic spines produced potential

219 ing combined with two-photon laser glutamate uncaging onto CWC spines.

220 Patterned uncaging opens new vistas in the study of signal integra

221 ecific LTP induction by two-photon glutamate uncaging or by synaptic stimulation, subthreshold stimul

222 cular smooth muscle cells via ex vivo Ca(2+) uncaging or in vivo optical activation produced only poo

223 lor experiments, e.g., when combining Ca(2+) uncaging or optogenetic stimulation with Ca(2+) imaging

224 city protocol, in which two-photon glutamate uncaging over a spine is paired with postsynaptic spikes

225 ramidal cells, we find that the amplitude of uncaging potentials at the soma is inversely proportiona

226 lly localized to the spine and occurred with uncaging potentials of different amplitudes and in spine

227 Uncaging potentials onto spines summed linearly, whereas

228 In practically all spines examined, uncaging potentials were significantly reduced by TTX.

229 nal chromaffin cells, we show that PI(4,5)P2 uncaging potentiates exocytosis and identify synaptotagm

230 The uncaging process is compatible with biological milieu an

231 marin chromophore influences the rate of the uncaging process, opening the way to exploiting these ne

232 ficant affinity for Mg(2+) to complicate the uncaging process.

233 epresenting an approximately 85-90% yield of uncaged product with a quantum yield phi(P) = 0.21.

234 e nAChRs were activated using a paired-pulse uncaging protocol, where the duration of the UV laser pu

235 ylphenol was not observed showing that free (uncaged) radical intermediates are not involved in the m

236 Mechanistic studies of the uncaging reaction implicate a photoredox pathway involvi

237 Here we report a near-IR light-initiated uncaging reaction sequence based on readily synthesized

238 in we report a novel bioorthogonal tetrazine uncaging reaction that harnesses tetrazine reactivity to

239 no effect on cytoplasmic [H(+)] in the H(+)-uncaging region, indicating that DIDS-sensitive transpor

240 Dual patch-clamp recording and Ca2+ uncaging revealed Ca2+-dependent corelease of ACh and GA

241 in a single spine using two-photon glutamate uncaging, RhoA and Cdc42 were rapidly activated in the s

242 ed immolative linker by Ru(II) conjugates to uncage rhodamine was achieved using different oligomeric

243 ere significant increases in the fraction of uncaging sites from which EPSCs could be evoked ("hot sp

244 ices showed that the proportion of glutamate uncaging sites from which excitatory postsynaptic curren

245 xially unsymmetrical silicon phthalocyanines uncage small molecules preferentially in a low-oxygen en

246 sprouting, a higher proportion of glutamate-uncaging spots in the granule cell layer evoked EPSCs in

247 enlargement resulting from a high-frequency uncaging stimulus into spine shrinkage, demonstrating th

248 Bioorthogonal uncaging strategies have recently emerged as an experime

249 A bioorthogonal uncaging strategy is presented, which is triggered by he

250 for the alignment and calibration of a focal uncaging system.

251 Low-energy light prompts photo-uncaging (t1/2 <1-2 min) and target-specific modificatio

252 the rapid sequestration of actin monomers by uncaged Tbeta4 and the consequent reduction in the diffu

253 Photochemical uncaging techniques use light to release active molecule

254 Uncaging technology and fluorescence microscopy are 'opt

255 al to its cleavage peptide and blue light to uncage the cleavage site.

256 Previously uncaging the compounds suitable for transient kinetic in

257 cantly diminished affinity for Cu2+, thereby uncaging the metal ion.

258 using brief, targeted exposure to UV light, uncaging the rhodamine and causing the particles in that

259 Phenol-containing small molecules are uncaged through sequential release of the C4'-amine and

260 We used two-photon glutamate uncaging to activate NMDA-Rs on individual dendritic spi

261 th local electrical stimulation or glutamate uncaging to analyze the effect of MOR activation on loca

262 We have used multisite two-photon glutamate uncaging to deliver different spatiotemporal input patte

263 enetics, two-photon microscopy and glutamate uncaging to examine D2R-dependent modulation of glutamat

264 Using two-photon microscopy and glutamate uncaging to examine individual synapses in the rat stria

265 -photon microscopy, and two-photon glutamate uncaging to examine subthreshold synaptic integration in

266 We used two-color, two-photon uncaging to fire and block action potentials from rat hi

267 e lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine tr

268 d somatic recordings and multisite glutamate uncaging to investigate the relationship between synapti

269 aser scanning photostimulation via glutamate uncaging to map excitatory and inhibitory synaptic input

270 We used two-photon RuBi-Glutamate uncaging to optically map how the largest population of

271 We then use local pharmacology and GABA uncaging to show that dendritic GABA(B)Rs also decrease

272 Here we use 2-photon glutamate uncaging to stimulate individual spines while monitoring

273 a2+] signaling, we used two-photon glutamate uncaging to stimulate NMDA-Rs on individual dendritic sp

274 g 2-photon Ca imaging and 2-photon glutamate uncaging, to examine how voltage-sensitive Ca channels (

275 Additionally, uncaging trains caused a reduction in the Ca(2+) accumul

276 Low-frequency uncaging trains induced Ca(2+)-dependent long-term depre

277 Finally, PI(4,5)P2 uncaging triggered the rapid fusion of a subset of readi

278 have developed an approach for sequentially uncaging two different phosphopeptides in one system, en

279 ng visual receptive field mapping, glutamate uncaging, two-photon Ca(2+) imaging, and genetic labelin

280 ng 3D digital holography and focal glutamate uncaging, voltage-sensitive dye, two-photon imaging, ele

281 To probe biological utility, thiol group uncaging was carried out using a peptide derived from th

282 This increase of calcium caused by calcium uncaging was followed by recovery to the prestimulated l

283 rpine-treated rats, laser-scanning glutamate uncaging was used to randomly and focally activate neuro

284 By applying patterned, two-photon glutamate uncaging, we found that single dendrites of cortical pyr

285 o quantify pH values upon UV irradiation and uncaging, we introduce a simple silica nanoparticle pH s

286 Using local uncaging, we photolyzed dextran-CANPE-HCC to release the

287 nique in combination with 2-photon glutamate uncaging, we show that neurofibromin, in which loss-of-f

288 Using two-photon uncaging, we show that this subcellular targeting strong

289 Using two-photon glutamate uncaging, we stimulated nascent spines on dendrites of r

290 Using two-photon glutamate uncaging, we then reveal that GABA(B) receptors strongly

291 receptor currents evoked by focal glutamate uncaging were both substantially reduced in these mice.

292 s evoked within approximately 100 ms of GABA uncaging were increased, while EPSCs evoked approximatel

293 s evoked approximately 300-600 ms after GABA uncaging were reduced compared to interleaved control sw

294 aged calcium probe o-nitrophenyl EGTA and UV uncaging were used to increase calcium in endocytic vacu

295 an enhanced version of two-photon glutamate uncaging, which preserves inhibitory synaptic transmissi

296 We then used two-photon glutamate uncaging, whole-cell recording, and Ca(2+) imaging to an

297 g of caged fluorescent dye after it has been uncaged with a laser pulse.

298 GTA, which has a negligible Mg(2+) affinity, uncages with a time constant of 10.3 ms, resulting in re

299 microscope, allowing us to combine patterned uncaging with imaging and electrophysiology.

300 Here we combine two-photon uncaging with two-photon imaging of a fluorescent label