ライフサイエンスコーパス: unfold

1 erparts, their early history is beginning to unfold.

2 panning residues 12 to 20 and 204 to 210 are unfolded.

3 protein that is highly soluble and natively unfolded.

4 ause its other interacting proteins are also unfolded.

5 acting the membrane, undergo surface-induced unfolding.

6 erminants to the volume changes upon protein unfolding.

7 dies, such as membrane processes and protein unfolding.

8 nality that is comparable to protein folding/unfolding.

9 trefoil knot against GFP, which prevents GFP unfolding.

10 lling force on the substrate and causing its unfolding.

11 in by approximately 10 kJ/mol, promoting its unfolding.

12 een shown to be crucial in urea-assisted RNA unfolding.

13 etergents from membrane proteins and protein unfolding.

14 stent with flexibility and facile reversible unfolding.

15 ar activities) that maintain proteostasis by unfolding aberrant and toxic proteins for refolding or p

16 nce into how the controlled talin rod domain unfolding acts as a key regulator of adhesion structure

17 ontribute to neurodegenerative disease, with unfolding, aggregation, and proteolytic cleavage as diff

18 ns requires the function of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape c

19 ich translocate across the outer membrane as unfolded amyloid precursors through a secretion system.

20 outer-membrane protein channel through which unfolded amyloid substrates are translocated.

21 arallel dimers, tetramers, and hexamers that unfold and polymerize into antiparallel filaments.

22 are not permanently folded but spontaneously unfold and refold on time scales that can span orders of

23 nase-off lesion of CCW B, the alpha1 helices unfold and the alpha2 helices form a tight two-helix coi

24 pe protein, whereas the N-terminal domain is unfolded and comprises an ensemble of random conformers,

25 We show that single proteins, kept unfolded and extended by a mechanical force, undergo acc

26 h, G4-DNA-forming conditions, a plurality of unfolded and folded species were present.

27 Even in unfolded and intrinsically disordered proteins, internal

28 -known function of J-domains of transferring unfolded and misfolded proteins to Hsp70.

29 ivo depends on the architectures of both the unfolded and native conformations.

30 histamine, but when Spy0125 was mechanically unfolded and subjected to forces of 50-350 piconewtons,

31 hydrophobic membrane bilayers, they must be unfolded and translocated by a membrane-embedded channel

32 connections between protein/peptide folding, unfolding and aggregation mechanisms, with the resulting

33 mation of EF-hand IV, leading to a transient unfolding and dissociation of helix H that becomes more

34 the molecular mechanism of thermally induced unfolding and dissociation of the Crcen-HsPrp40Ap comple

35 s related to loss of helical structure, weak unfolding and ejection of the prostetic group.

36 earing the p.Arg284Pro mutation was prone to unfolding and exhibited reduced glycosylation activity a

37 In the present study, we have determined the unfolding and folding kinetics of 10 FNIII domains.

38 Here, equilibrium unfolding and hydrogen/deuterium exchange monitored by m

39 netics data gave the kinetics of spontaneous unfolding and refolding in zero urea.

40 hines couple hydrolysis of ATP to mechanical unfolding and translocation of proteins following recogn

41 vestigation into the mechanism by which HdeA unfolds and activates.

42 heterohexameric AAA(+) ATPase module, which unfolds and translocates substrates into the CP.

43 (for example, cleavage, binding, folding and unfolding) and biological targets in in vitro and in viv

44 that the cortactin repeats are intrinsically unfolded, and the experimentally observed radius of gyra

45 nes of the HSP100 family help with substrate unfolding, and additional accessory proteins are believe

46 triphosphatase (ATPase) Mpa, which captures, unfolds, and translocates protein substrates into the Mt

47 icipating strongly in native contacts during unfolding are distributed differently in CheY than in th

48 within and between such networks dynamically unfold as a function of threat-related arousal remains u

49 ason for this is unclear, as the arms do not unfold as coaxial stacks, and thus its melting behavior

50 formational broadening and primarily gradual unfolding, as opposed to the distinct transitions throug

51 In vitro unfolding assays reveal that addition of O-glucose or O-

52 Thermally induced unfolding at 37 degrees C and concomitant functional ina

53 successfully characterized the force induced unfolding behavior of all cohesins from the scaffoldin S

54 lar interactions between residues within the unfolded beta-domain.

55 The I2 state is more unfolded, but it retains some residual secondary structu

56 re, we investigate whether globular proteins unfolded by SDS can be refolded upon addition of C12E8 a

57 g AAA+ chaperones exert ATP-fueled substrate unfolding by threading through a central pore.

58 PyoS2(NTD) induces unfolding by TonB1 of a force-labile portion of the plug

59 enhances the propensity of the intrinsically unfolded C-terminal domains to drive pathological aggreg

60 of cryptic side chains exposed by mechanical unfolding can be slowed by the addition of antioxidants

61 rth system model in order to reconstruct the unfolding carbon-cycle dynamics during the event.

62 Instead, the stability is conferred by the unfolded central domain of CENP-C and the folded N-termi

63 motions expected for an unstructured, fully unfolded chain.

64 s to protein folding homeostasis by engaging unfolded client proteins in a process that is tightly co

65 second longest-lived metastable state was an unfolded, compact monomer with little similarity to the

66 A protein forced to be continuously unfolded completely loses its ability to contract by fol

67 sensor protein, the molecular origins of its unfolding complexity, highlights the role of functional

68 Although everyday experiences unfold continuously over time, shifts in context, or eve

69 ocyte to alveolar macrophage differentiation unfolds continuously over the course of fibrosis and its

70 of the regulatory particle is to recognize, unfold, deubiquitylate, and translocate substrates into

71 nd POFUT1 can distinguish between folded and unfolded EGF repeats raised the possibility that they pa

72 t the need for a transition across the major unfolding energy barrier.

73 ion between BiP AMPylation and the burden of unfolded ER proteins suggests a post-translational mecha

74 reexisting salt bridge, initiating a partial unfolding event and promoting new protein interactions.

75 Unfolding events were registered in the phase diagram of

76 Urea-unfolding experiments and MM/GBSA calculations converge

77 cal relevance, because these proteins remain unfolded for extended periods of time during their bioge

78 ferent mechanical proteins in terms of their unfolding forces.

79 denaturation above zero degrees, because the unfolded form at low temperature is more expanded than t

80 ding pathway, and we are able to assign each unfolded form observed in our gas-phase data set to the

81 The unfolding free energy of the consensus-HD is 5 kcal.mol(

82 We also show that CST unfolds G-quadruplex structures, thus providing a mechan

83 y unanticipated structural role for natively unfolded GLFG repeats as Velcro to link NPC subcomplexes

84 S sodium dodecyl sulfate (SDS) denatures and unfolds globular proteins under most conditions.

85 with a hybrid i-motif/hairpin species and an unfolded hairpin species.

86 Thermally or chemically induced unfolding has previously been employed to measure alpha-

87 lose to the active site in the first step of unfolding, i.e., the N-I1 transition.

88 , they can also be deceived about how events unfold in time.

89 on trustworthiness perception from faces has unfolded in a way that is strikingly reminiscent of Juss

90 at readily assemble in vitro but shown to be unfolded in vivo.

91 Both techniques revealed the degree of unfolding in protein structure increased when fish prote

92 DDM) protect bovine serum albumin (BSA) from unfolding in SDS.

93 lecule in a stack of two beta roll molecules unfolds in a step-wise fashion mainly from the C termina

94 how auditory processing of continuous speech unfolds in the human brain.

95 tly, it is unclear how this critical pathway unfolds, in particular, where and when TLS occurs on eac

96 ly characterized the stable equilibrium urea unfolding intermediate of V75D at the ensemble level usi

97 ical utility by first resolving a calmodulin unfolding intermediate previously undetected by AFM and

98 y for immunoglobulin light-chains populating unfolded intermediates that result in amyloid formation,

99 induce conformational collapse and distinct unfolding intermediates for all four linkage types, prov

100 o the distinct transitions through gas-phase unfolding intermediates observed of K6-, K11-, and K63-l

101 charge states in mass spectra and generating unfolded ion structures, yet key details of its mechanis

102 est that a likely mechanism for triple helix unfolding is intermolecular shearing of collagen alpha-c

103 It is seen that CPR3 unfolding is reversible and proceeds via two intermediat

104 ons of proteins exists, beyond which thermal unfolding is triggered.

105 ng further along the target protein, thereby unfolding it.

106 he biophysical mechanisms of RNA folding and unfolding, its interactions with ligands, and its functi

107 s, we observed that CaM preferentially binds unfolded K-Ras4B hypervariable regions (HVRs) and not al

108 e trigger loop (TL), which cycles between an unfolded loop and an alpha-helical hairpin [trigger heli

109 s requiring virtually no mechanical force to unfold (<10 piconewtons (pN)), suggesting that these mol

110 Unfolded maps of histologically determined boundaries we

111 ructure suggests a processive hand-over-hand unfolding mechanism, where each VAT subunit releases the

112 Studies of protein unfolding mechanisms are critical for understanding prot

113 increases the precision of determination of unfolding mechanisms, free energies, temperatures, and h

114 A pathway from the natively unfolded microtubule-associated protein Tau to a highly

115 uted computing network to study the folding, unfolding, misfolding and conformational plasticity of t

116 c effects and yields novel insights into the unfolding molecular changes in the brain of a new schizo

117 tudy, we describe an approach of progressive unfolding of a target protein using a gradient of denatu

118 draw new specific conclusions regarding the unfolding of albumins in the gas phase, as well as more

119 romosome capping structure can result in the unfolding of existing G-quadruplexes which can lead to t

120 for IsdB heme transfer from Hb that involves unfolding of Hb and heme iron ligand exchange.

121 We analyzed the acid-induced unfolding of HdeA using NMR spectroscopy and fluorescenc

122 1-microsecond resolution, we reexamined the unfolding of individual bacteriorhodopsin molecules in n

123 e-molecule force spectroscopy to measure the unfolding of individual chromatin fibers that are revers

124 four-way junction increases the cooperative unfolding of its stems, although the reason for this is

125 2 binding and couple ATP hydrolysis to local unfolding of Mad2.

126 onin-mediated protein folding, where partial unfolding of misfolded intermediates plays a key role.

127 ing hexamers that hydrolyze ATP to power the unfolding of protein substrates and their translocation

128 Force induced unfolding of talin rod subdomains has been proposed to a

129 higher salt concentration, the simultaneous unfolding of the above domains is lost, resulting in the

130 ion, we observed that both mutations lead to unfolding of the C-terminal region in the t-SNARE comple

131 7) s(-1) Molecular simulations revealed that unfolding of the disulfide bond-lacking variant is initi

132 We induce targeted mechanical unfolding of the G-quadruplex while leaving the nanocage

133 n of the PC4-G4DNA complex did not result in unfolding of the G4DNA structure.

134 sheds light on the origin and developmental unfolding of the link between language and cognition in

135 This tension is resolved by reversible unfolding of the linker, which is a singular mechanism t

136 f high-quality data, defined as the complete unfolding of the polyprotein.

137 the above domains is lost, resulting in the unfolding of the three separate stems.

138 ture of cell surfaces and highlights how the unfolding of these convoluted surfaces contributes to ph

139 in vivo or denaturants in vitro trigger the unfolding of this domain, thereby leaving the cleavage s

140 Free energy profiles corresponding to the unfolding of Trp-cage miniprotein in the presence and ab

141 Through calibrated unfolding of two protein-ligand systems: cAMP-bound regu

142 e present a detailed characterization of the unfolding of yeast mitochondrial cyclophilin (CPR3) indu

143 the ribosomal footprint, the free energy to unfold only the overlapping structure controlled the ext

144 cificity at protein interfaces through local unfolding or conformational change.

145 how that degron placement can change whether unfolding or translocation is rate limiting, and establi

146 ity and crop production that are expected to unfold over the next 20 to 30 years.

147 world around us is replete with stimuli that unfold over time.

148 Parallel factor analysis (PARAFAC) and unfolded-partial least squares coupled to residual bilin

149 The experimental data reveal the unfolding pathway in unprecedented detail.

150 at human albumin follows a domain associated unfolding pathway, and we are able to assign each unfold

151 g a complex mechanism in HdeA's acid-induced unfolding pathway, as previously postulated from molecul

152 of FimG is due to a shift in the mechanical unfolding pathway.

153 lated by the program Flexible-Meccano for an unfolded peptide of this length.

154 lysis, which allows nonspecific digestion of unfolded peptides by trypsin.

155 ng mechanisms as well as the interactions of unfolded polypeptide chains with other cell components.

156 eassemble in a cooperative manner from their unfolded polypeptide subunits, without biological chaper

157 plasmic transport is an assembly of natively unfolded polypeptides, which contain phenylalanine-glyci

158 their shape in response to external stimuli unfold possibilities for more efficient and versatile pr

159 oplasmic reticulum (ER) stress, triggered by unfolded protein accumulation in the ER, contributes to

160 simulations, and theory to the same system: unfolded protein L.

161 y caused by the aggregation of the initially unfolded protein molecules.

162 ich leads to activation of the mitochondrial unfolded protein response (mitoUPR).

163 , which regulates mitochondrial dynamics and unfolded protein response (UPR(mt)) in the host.

164 the SIRT/FOXO/SOD2 axis of the mitochondrial unfolded protein response (UPR(mt)).

165 stress-response mechanism, the mitochondrial unfolded protein response (UPR(MT)).

166 The unfolded protein response (UPR) adjusts the cell's prote

167 induction of the IRE1alpha/XBP1 axis of the unfolded protein response (UPR) and culminating in termi

168 ), which is associated with induction of the unfolded protein response (UPR) and disruption of autoph

169 f procollagen I ER export could activate the unfolded protein response (UPR) and drive HSC apoptosis.

170 g to light the distinctive complexity of the unfolded protein response (UPR) in plants.

171 ulate in the endoplasmic reticulum (ER), the unfolded protein response (UPR) increases ER-protein-fol

172 The unfolded protein response (UPR) is a cytoprotective path

173 The unfolded protein response (UPR) is a shared pathway util

174 The unfolded protein response (UPR) is activated to cope wit

175 The unfolded protein response (UPR) is essential for physiol

176 The unfolded protein response (UPR) monitors and adjusts the

177 at KDM1A inhibitors induce activation of the unfolded protein response (UPR) pathway.

178 animals use a survival pathway known as the unfolded protein response (UPR) that signals between the

179 We have investigated the unfolded protein response (UPR) to endoplasmic reticulum

180 d a set of signaling mechanisms known as the unfolded protein response (UPR) to increase the folding

181 the ER folding capacity, which activates the unfolded protein response (UPR) to restore ER homeostasi

182 the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) to restore homeostasis.

183 endoplasmic reticulum (ER) and activate the Unfolded Protein Response (UPR) to trigger adaptive resp

184 RE1) is the most conserved transducer of the Unfolded Protein Response (UPR), a surveillance mechanis

185 he perinuclear area led to activation of the unfolded protein response (UPR), suggesting that specifi

186 uding the endoplasmic reticulum (ER)-induced unfolded protein response (UPR), the ubiquitin-proteasom

187 signaling pathways, collectively termed the unfolded protein response (UPR), to maintain a productiv

188 This activates the unfolded protein response (UPR), which alters the expres

189 metabolic homeostasis via the activation of unfolded protein response (UPR), which is instructed by

190 on the set of genes that is activated by the unfolded protein response (UPR).

191 s endoplasmic reticulum stress that leads to unfolded protein response (UPR).

192 ve phosphorylation, vesicle traffic, and the unfolded protein response (UPR).

193 of a second proteotoxic stress response, the unfolded protein response (UPR).

194 for cell adaptation and survival, named the unfolded protein response (UPR).

195 athway leading to ER stress and an activated unfolded protein response (UPR).

196 tute potential cross-talk between HD and the unfolded protein response (UPR).

197 actor that is secreted but also mediates the unfolded protein response acting intracellularly.

198 nclude that ER Ca(2+) drainage and sustained unfolded protein response activation are key for initiat

199 resulted in vigorous activation of both the unfolded protein response and ER stress accompanied by i

200 Omega-3 fatty acids increase the unfolded protein response and improve amyloid-beta phago

201 bs target cell-surface GRP78, activating the unfolded protein response and inducing tumor cell prolif

202 tein aggregation activates the mitochondrial unfolded protein response and PINK1-Parkin-mediated mito

203 ich regulates the endoplasmic reticulum (ER) unfolded protein response and promotes cell survival upo

204 utrient deficit acted in turn to trigger the unfolded protein response and the amino acid response, l

205 Whereas activation of some components of the unfolded protein response and the pro-apoptotic mediator

206 yeast that chronic VPA treatment induces the unfolded protein response due to increasing ceramide lev

207 reticulum stress, suggesting that an altered unfolded protein response is unlikely to account for the

208 tment-specific stress responses, such as the unfolded protein response of the endoplasmic reticulum (

209 through the PERK/eIF2α-P branch of the unfolded protein response plays a critical role in contr

210 zed acinar cells by suppressing the adaptive unfolded protein response signaling pathway.

211 We investigated the role of the core unfolded protein response transcription factor X-box bin

212 that ClpXP is required for the mitochondrial unfolded protein response, additional roles for ClpXP in

213 NA repair, mTORC1 signaling, features of the unfolded protein response, and altered cholesterol homeo

214 GRP78 is widely used as a marker of the unfolded protein response, associated with sustained ER

215 athogens target innate immune signaling, the unfolded protein response, autophagy, and cellular metab

216 biogenesis, triggering of the mitochondrial unfolded protein response, loss of mitochondrial membran

217 subfamily B couples translation rate to the unfolded protein response, which has implications for un

218 ed diverse cellular processes, including the unfolded protein response, Wnt signalling and RNA metabo

219 cell death were associated with a sustained unfolded protein response.

220 ins to populate the organelle, eliciting the unfolded protein response.

221 paB signalling, antigen presentation and the unfolded protein response.

222 t hepatocytes, resulting in induction of the unfolded protein response.

223 ATF6 is a key regulator of the unfolded protein response.

224 induction of caspase processing but not the unfolded protein response.

225 nd non-canonical mRNA splicing in the fungal unfolded protein response.

226 uce the activation of 1 of the 3 branches of unfolded protein responses, the inositol-requiring enzym

227 stranslation was insufficient to activate an unfolded protein stress response.

228 ibute to UPR activation, but deletion of the unfolded protein stress-sensing luminal domain of the UP

229 a tight helix that grips an 80 A stretch of unfolded protein.

230 /-) macrophages attenuates the mitochondrial unfolded-protein response (mtUPR) as measured by express

231 to explore how perturbants commonly used to unfold proteins (urea, force, and temperature) affect th

232 We demonstrate that WT p97 can unfold proteins and that this activity is dependent on t

233 the mechanism by which chemical denaturants unfold proteins is crucial; this study explores the natu

234 mRNA for the transcription factor Hac1 when unfolded proteins accumulate in the endoplasmic reticulu

235 When unfolded proteins accumulate in the endoplasmic reticulu

236 o sets of measurements for the dimensions of unfolded proteins and IDPs in the absence of chemical de

237 Structural and dynamic investigations of unfolded proteins are important for understanding protei

238 Peptides and unfolded proteins bind to hIRE1alpha LD's MHC-like groov

239 Unfolded proteins compete for BiP and restore IRE1(LD) t

240 that ClpXP is dispensable for degradation of unfolded proteins in S. aureus.

241 Endoplasmic reticulum stress causes unfolded proteins to populate the organelle, eliciting t

242 he molecular origins of internal friction in unfolded proteins using atomistic simulations, coarse-gr

243 stress arises from accumulation of misfolded/unfolded proteins when protein load overwhelms the ER fo

244 esolution structures of their complexes with unfolded proteins.

245 sps) prevent the irreversible aggregation of unfolding proteins by integrating into forming aggregate

246 oth proteins exhibited similar kinetics, the unfolding rate constant changed upon deletion of the dis

247 odynamic stability correlates primarily with unfolding rate rather than folding rate.

248 The unfolding rates correlate well with the thermodynamic st

249 RBC spectrin cytoskeleton, including domain unfolding/refolding.

250 We considered that the unfolded region may alleviate the steric hindrance produ

251 urther analyses revealed that the N-terminal unfolded region of cargo proteins is critical for their

252 et their role for protein stability, and for unfolding, remains elusive.

253 Although sentences unfold sequentially, one word at a time, most linguistic

254 se, showing a specific and sensitive protein unfolding signature.

255 Moreover, proteins that unfold slower are more resistant to proteolysis.

256 Under conditions promoting protein unfolding, small heat shock proteins (sHsps) prevent the

257 one or a few power strokes are sufficient to unfold some protein domains.

258 Populating transient and partially unfolded species is a crucial step in the formation and

259 The partially unfolded species possess an unstructured beta-domain and

260 ility and its kinetic accessibility from its unfolded state (U state).

261 residue-level PP2 dihedral angle bias in the unfolded state and PP2 helical structure in the native s

262 lecule spectroscopy, we have scrutinized the unfolded state of outer-membrane phospholipase A (OmpLA)

263 sampling and a better representation of the unfolded state of the protein.

264 ures spanning the spectrum from the extended unfolded state to the native fold, yielded nearly 120 mu

265 quilibrium away from the extended, partially unfolded state towards a compact, pre-organized conforma

266 the case of outer-membrane proteins (OMPs), unfolded-state properties are of particular physiologica

267 modynamic equilibrium between the folded and unfolded states of a protein can pinpoint residues of cr

268 thermodynamic equilibrium between native and unfolded states of proteins.

269 onverting and conformationally heterogeneous unfolded states that lack the fast chain-reconfiguration

270 ions allowed RACER to distinguish folded vs. unfolded states.

271 , all force fields predict that Abeta adopts unfolded structure dominated by turn and random coil con

272 amatically reduced to only 2% of that of the unfolded structure.

273 tweezers, together with traditional protein unfolding studies to explore how perturbants commonly us

274 AAA+ unfoldases are thought to unfold substrate through the central pore of their hexam

275 form a closed spiral staircase encircling an unfolded substrate, directing it toward the flat, symmet

276 bone, and frontotemporal dementia in humans unfolds substrate faster, suggesting that excess activit

277 and is phosphorylated on two residues at its unfolded tail domain.

278 We find that ClpXP and ClpAP unfold the wild-type titin(I27) domain and a destabilize

279 To unfold their full and optimized catalytic activity and s

280 folded proteins, the force of translocation unfolds them mechanically.

281 KBP domain causes the target domain to first unfold, then refold via domain swap.

282 metric techniques to investigate the folding/unfolding thermodynamics of two three-way junctions with

283 e predictions of volume changes upon protein unfolding, thus paving the way to proteome-wide computat

284 found that stabilizing either the folded or unfolded TL conformation decreased termination rate.

285 how the consequences of this genetic insult unfold to impair neurodevelopment.

286 garding the sensitivity of collision induced unfolding to changes in protein primary and tertiary str

287 are consistent with a two-state, cooperative unfolding transition having a melting temperature of Tm

288 re modulations while undergoing a continuous unfolding transition.

289 offered insight into the process of cohesin unfolding under force.

290 ipid binding, show increased stability (less unfolding) upon ligand binding.

291 hanical stress, we studied its force-induced unfolding using single molecule atomic force microscopy

292 in light chain kinase at dramatically higher unfolding velocities than in previous AFM studies.

293 the avidin tetramer toward dissociation and unfolding, via both its effects on charge state distribu

294 Since it is unknown how such processes unfold, we developed a method to dynamically track level

295 In describing how the renaissance has unfolded, we attempt to describe different points of vie

296 ere, we use single-molecule chemo-mechanical unfolding, which combines force and urea using the optic

297 to watch this retrieval into working memory unfold with high temporal resolution.

298 e over time due to denaturation, as proteins unfold with loss of secondary and tertiary structure.

299 ed proteins fold with very similar rates but unfold with rates that differ up to three orders of magn

300 ctivity similar to the wild type enzyme, and unfolds with a Tm of 53 degrees C.