ライフサイエンスコーパス: unfolding

1 rocarbon groups and amide oxygens exposed in unfolding).

2 trefoil knot against GFP, which prevents GFP unfolding.

3 lling force on the substrate and causing its unfolding.

4 emperature dependence of volume changes upon unfolding.

5 to be more specific about their cooperative unfolding.

6 een shown to be crucial in urea-assisted RNA unfolding.

7 energy of nucleotide hydrolysis to substrate unfolding.

8 in by approximately 10 kJ/mol, promoting its unfolding.

9 eling activities that were not coupled to GQ unfolding.

10 etergents from membrane proteins and protein unfolding.

11 iates as in energetically uphill equilibrium unfolding.

12 nce and is not initiated simply by substrate unfolding.

13 se of the sequence of events observed during unfolding.

14 to both guanidine hydrochloride and thermal unfolding.

15 stent with flexibility and facile reversible unfolding.

16 ical trimers added exceptional resistance to unfolding.

17 acting the membrane, undergo surface-induced unfolding.

18 erminants to the volume changes upon protein unfolding.

19 dies, such as membrane processes and protein unfolding.

20 nality that is comparable to protein folding/unfolding.

21 scence is lost during the earliest events in unfolding, 3.5 ms before secondary structure is disrupte

22 ar activities) that maintain proteostasis by unfolding aberrant and toxic proteins for refolding or p

23 s during homologous recombination but its GQ unfolding activity are not known.

24 n aggregates is promoted and how this potent unfolding activity is prevented from doing collateral da

25 hich restricts the potentially toxic protein unfolding activity of Hsp104 to the disaggregation proce

26 Such unfolding activity of RHAU leads to efficient annealing

27 terically controls the mobility and thus the unfolding activity of the ATPase modules.

28 ese studies demonstrated a RECQ5-mediated GQ unfolding activity that was an order of magnitude weaker

29 nce into how the controlled talin rod domain unfolding acts as a key regulator of adhesion structure

30 in 3D structure and fluidic paths-by folding/unfolding add functionality to EPADs (e.g., controlled t

31 ontribute to neurodegenerative disease, with unfolding, aggregation, and proteolytic cleavage as diff

32 is evidence that monomerization and partial unfolding allow the chaperone to bind to proteins denatu

33 ns requires the function of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape c

34 connections between protein/peptide folding, unfolding and aggregation mechanisms, with the resulting

35 nisms use molecular chaperones to combat the unfolding and aggregation of proteins.

36 s in the Arrhenius plots, distinguishing the unfolding and aggregation phases of the denaturation pro

37 before it reaches GFP, enabling the complete unfolding and degradation of the substrate.

38 ze substrates and deubiquitylate them before unfolding and degradation.

39 mation of EF-hand IV, leading to a transient unfolding and dissociation of helix H that becomes more

40 the molecular mechanism of thermally induced unfolding and dissociation of the Crcen-HsPrp40Ap comple

41 cular dichroism spectroscopy suggest partial unfolding and domain destabilization in the presence of

42 s related to loss of helical structure, weak unfolding and ejection of the prostetic group.

43 ts, whereas other LCs are not as amenable to unfolding and endoproteolysis and are therefore aggregat

44 earing the p.Arg284Pro mutation was prone to unfolding and exhibited reduced glycosylation activity a

45 We measure unfolding and folding force-dependent kinetic rates from

46 In the present study, we have determined the unfolding and folding kinetics of 10 FNIII domains.

47 The unfolding and folding of protein barnase has been extens

48 the height of the kinetic barrier mediating unfolding and folding transitions, finding good agreemen

49 stantial conformational rearrangement during unfolding and forms an expanded dry-MG.

50 Here, equilibrium unfolding and hydrogen/deuterium exchange monitored by m

51 table at low temperatures, resulting in cold unfolding and impairment of normal biological function.

52 tionship between the chaperone's own partial unfolding and its ability to bind other partially folded

53 to obtain detailed understanding about apoE unfolding and its changes upon ligand binding.

54 ity is consistent with the significant local unfolding and loss of hydrogen bonding seen in molecular

55 Thus, IRE1alpha senses protein unfolding and metabolic and immunological states, and co

56 recording full energetic profiles of protein unfolding and permitting data fitting based on unfolding

57 netics data gave the kinetics of spontaneous unfolding and refolding in zero urea.

58 Here we report the force-dependent unfolding and refolding kinetics of all talin rod domain

59 ssipate mechanical energy through mechanical unfolding and refolding of isopeptide bond-delimited pol

60 min) on the relative molecular mass, protein unfolding and secondary structure of quinoa globulins wa

61 atomic force spectroscopy to determine their unfolding and stability.

62 orms disulfide-bonded dimers that experience unfolding and then assemble into aggregate particles (AP

63 Mechanisms of ClpY-assisted unfolding and translocation are distinct from those resu

64 he molecular basis for how clamps facilitate unfolding and translocation is poorly understood.

65 We find conserved aspects of unfolding and translocation mechanisms by allosteric Clp

66 system to elucidate the molecular details of unfolding and translocation of an alpha/beta model prote

67 hines couple hydrolysis of ATP to mechanical unfolding and translocation of proteins following recogn

68 ion of mechanical forces onto SPs to promote unfolding and translocation.

69 (for example, cleavage, binding, folding and unfolding) and biological targets in in vitro and in viv

70 nes of the HSP100 family help with substrate unfolding, and additional accessory proteins are believe

71 thermodynamic stability, reduce the rate of unfolding, and enhance the A1 domain's resistance to lim

72 ctural rigidity and resistance to mechanical unfolding, and instead strongly support more recent stud

73 tes when undergoing force-induced mechanical unfolding, and it can bind and recruit cytoskeletal prot

74 icipating strongly in native contacts during unfolding are distributed differently in CheY than in th

75 nt of a I27 molecule at the moment of module unfolding are equal to 4+/-1.5mN/m, and the typical valu

76 formational broadening and primarily gradual unfolding, as opposed to the distinct transitions throug

77 In vitro unfolding assays reveal that addition of O-glucose or O-

78 Thermally induced unfolding at 37 degrees C and concomitant functional ina

79 Irreversible protein unfolding at 37 degrees C was observed, and we propose t

80 successfully characterized the force induced unfolding behavior of all cohesins from the scaffoldin S

81 of DMSO concentration on the dissociation or unfolding behavior of proteins.

82 t trends in the rates of protein folding and unfolding; both modern RNases H evolved to be more kinet

83 s G-quadruplexes and mediates their stepwise unfolding, but also explain how FANCJ chooses between su

84 number of studies reporting G-quadruplex DNA unfolding by helicase enzymes has rapidly increased.

85 Deubiquitination by Rpn11 and ubiquitin unfolding by the ATPases are in direct competition.

86 g AAA+ chaperones exert ATP-fueled substrate unfolding by threading through a central pore.

87 PyoS2(NTD) induces unfolding by TonB1 of a force-labile portion of the plug

88 g drastically decreases the cooperativity of unfolding by trapping the closed flap conformation in a

89 howed that the computational accuracy of the unfolding calculations was similar to the accuracy of pr

90 ons suggest that volume changes upon protein unfolding can be both positive and negative.

91 of cryptic side chains exposed by mechanical unfolding can be slowed by the addition of antioxidants

92 rth system model in order to reconstruct the unfolding carbon-cycle dynamics during the event.

93 ration of about 17% compared to the complete unfolding caused by 6M guanidine hydrochloride.

94 ectrometry (ESI-IM-MS) and collision-induced unfolding (CIU) analysis of four diUbq ions to provide n

95 s can affect the extent of collision-induced unfolding (CIU) and collision-induced dissociation (CID)

96 ced dissociation (CID) and collision-induced unfolding (CIU) as monitored by nanoelectrospray ionizat

97 Here, we show using collision-induced unfolding (CIU) ion mobility-mass spectrometry (IM-MS) t

98 sensor protein, the molecular origins of its unfolding complexity, highlights the role of functional

99 tery, conformational spread, induced folding/unfolding, conformational memory, the hierarchical assem

100 Hsp104(N) fragments Sup35 prions without unfolding cross-beta structure or releasing soluble Sup3

101 The program takes into account the protein-unfolding curve and generates propensities using calcula

102 ones control the cellular folding, assembly, unfolding, disassembly, translocation, activation, inact

103 dc48 might couple ATP hydrolysis to forcible unfolding, dissociation, or remodeling of cellular clien

104 t the need for a transition across the major unfolding energy barrier.

105 parated by a central ionic layer, with total unfolding energy of approximately 17 kBT, where kB is th

106 d increases the stability of CYP142A1 toward unfolding even up to 40% DMSO.

107 reexisting salt bridge, initiating a partial unfolding event and promoting new protein interactions.

108 Unfolding events were registered in the phase diagram of

109 Urea-unfolding experiments and MM/GBSA calculations converge

110 ep-Tactin is generally applicable to protein unfolding experiments.

111 We observed slow cooperative unfolding/folding events within the C-terminal helices o

112 on in the transition temperature for helical unfolding for an H223Q mutant starts at less acidic cond

113 ed with its reduced counterpart, whereas the unfolding force of I91 domains experiences a decrease of

114 e hanging cohesins showed considerably lower unfolding forces than the bridging cohesins, which are s

115 ferent mechanical proteins in terms of their unfolding forces.

116 At neutral pH, the unfolding free energy in cells is comparable to that in

117 The unfolding free energy of the consensus-HD is 5 kcal.mol(

118 Deficiencies in unfolding GQ are known to result in DNA breaks and genom

119 Thermally or chemically induced unfolding has previously been employed to measure alpha-

120 As the age of molecular biology was unfolding, I pursued a PhD in nutritional biochemistry w

121 lose to the active site in the first step of unfolding, i.e., the N-I1 transition.

122 l regime is attained in proximity of thermal unfolding in all solvents that we tested.

123 oton exchange to quantify the free energy of unfolding in cells.

124 We find that Cnu displays probe-dependent unfolding in equilibrium, graded increase in structural

125 ability to deal with the geochemical changes unfolding in our coastal ocean.

126 Both techniques revealed the degree of unfolding in protein structure increased when fish prote

127 DDM) protect bovine serum albumin (BSA) from unfolding in SDS.

128 t detailed mechanism for multidomain protein unfolding in the gas phase, and highlights key similarit

129 to a monomeric intermediate precedes protein unfolding, in contrast to the typical two-state model de

130 random coil structure, resulting in protein unfolding, inhibition of PDI reductase and isomerase act

131 ion mechanisms by allosteric ClpY, including unfolding initiated at the tagged C-terminus and translo

132 This Perspective elaborates on the currently unfolding interest in integrating unorthodox non-covalen

133 ly characterized the stable equilibrium urea unfolding intermediate of V75D at the ensemble level usi

134 ical utility by first resolving a calmodulin unfolding intermediate previously undetected by AFM and

135 (32) and Cys(41), was likely to stabilize an unfolding intermediate prone to intermolecular interacti

136 the common belief that chaperones recognize unfolding intermediates by their hydrophobic nature, we

137 induce conformational collapse and distinct unfolding intermediates for all four linkage types, prov

138 o the distinct transitions through gas-phase unfolding intermediates observed of K6-, K11-, and K63-l

139 No fluorescence is observed from the unfolding intermediates or the ensemble of compact and e

140 The study suggested that protein unfolding is a more important phenomenon than solubiliza

141 results suggest that cyt c major structural unfolding is associated with the distancing of the N- an

142 hed in the unfolded states of both proteins, unfolding is completely reversible for HiGlpG but only p

143 tructural rationale for the observation that unfolding is hierarchical and describable by exponential

144 est that a likely mechanism for triple helix unfolding is intermolecular shearing of collagen alpha-c

145 It is seen that CPR3 unfolding is reversible and proceeds via two intermediat

146 ons of proteins exists, beyond which thermal unfolding is triggered.

147 perative transition, corresponding to global unfolding, is observed at higher denaturant concentratio

148 ng further along the target protein, thereby unfolding it.

149 he biophysical mechanisms of RNA folding and unfolding, its interactions with ligands, and its functi

150 The mechanosensation via receptor unfolding may be applicable for many other cell adhesion

151 c GQ structures by using a common repetitive unfolding mechanism that provides a robust resolving pow

152 ructure suggests a processive hand-over-hand unfolding mechanism, where each VAT subunit releases the

153 Studies of protein unfolding mechanisms are critical for understanding prot

154 s unique tools for dissecting the mechanical unfolding mechanisms of such proteins.

155 increases the precision of determination of unfolding mechanisms, free energies, temperatures, and h

156 e thermodynamically most stable state before unfolding-mediated dissociation has time to take place,

157 uted computing network to study the folding, unfolding, misfolding and conformational plasticity of t

158 c effects and yields novel insights into the unfolding molecular changes in the brain of a new schizo

159 eat-shock proteins (sHsps) apparently act as unfolding nanomachines capable of converting functional

160 s directly reveal that collagen triple helix unfolding occurs during tensile loading of collagenous t

161 tivation of the chaperone Hsp33 requires the unfolding of a central linker region.

162 a complete thermodynamic description of the unfolding of a DNA four-way junction (FWJ) and its appro

163 ing can be greatly accelerated: the constant unfolding of a protein for hours to days is equivalent t

164 aMD) simulations are used to investigate the unfolding of a stack of two beta roll molecules, (GAGAGA

165 hasticity can be linked to cycles of folding/unfolding of a stem-loop structure, or to interconversio

166 tudy, we describe an approach of progressive unfolding of a target protein using a gradient of denatu

167 ption model that explicitly accounts for the unfolding of adsorbed DOM.

168 draw new specific conclusions regarding the unfolding of albumins in the gas phase, as well as more

169 Comparison between unfolding of an NMS construct and that of truncated G-qu

170 Here, we investigate the mechanical unfolding of cold shock protein B (Csp), a showcase two-

171 Remarkably, equilibrium unfolding of dimeric FoxP1 follows a three-state N2 <-->

172 These observations identify the unfolding of dissociated PTS1 as a trigger for its ERAD-

173 e present a thermodynamic description of the unfolding of DNA intramolecular three-way junctions.

174 romosome capping structure can result in the unfolding of existing G-quadruplexes which can lead to t

175 for IsdB heme transfer from Hb that involves unfolding of Hb and heme iron ligand exchange.

176 We analyzed the acid-induced unfolding of HdeA using NMR spectroscopy and fluorescenc

177 Denaturant-induced unfolding of helical membrane proteins provides insights

178 We found a temperature-dependent unfolding of Hsf1 in the regulatory region happening con

179 king studies, we now reveal that the partial unfolding of Hsp33's linker region facilitates client bi

180 1-microsecond resolution, we reexamined the unfolding of individual bacteriorhodopsin molecules in n

181 e-molecule force spectroscopy to measure the unfolding of individual chromatin fibers that are revers

182 at ANGPTL4 inactivates LPL by catalyzing the unfolding of its hydrolase domain; (2) that binding to G

183 four-way junction increases the cooperative unfolding of its stems, although the reason for this is

184 catalytic activity by mitigating the global unfolding of LPL's catalytic domain.

185 2 binding and couple ATP hydrolysis to local unfolding of Mad2.

186 esults sharpen the picture of the mechanical unfolding of membrane proteins and, more broadly, enable

187 onin-mediated protein folding, where partial unfolding of misfolded intermediates plays a key role.

188 rimental measurements of volume changes upon unfolding of model proteins lysozyme and ribonuclease A,

189 The reversible equilibrium unfolding of native Cyt c steps up through these interme

190 ing hexamers that hydrolyze ATP to power the unfolding of protein substrates and their translocation

191 SA signals increased also significantly upon unfolding of S. aureus or E. coli antigens, whereas unfo

192 Here, we analyze the folding and unfolding of T4 lysozyme with optical tweezers under a c

193 Force induced unfolding of talin rod subdomains has been proposed to a

194 higher salt concentration, the simultaneous unfolding of the above domains is lost, resulting in the

195 lix formation by up to 50% and slow down the unfolding of the alpha-helix, whereas salt bridges with

196 ion, we observed that both mutations lead to unfolding of the C-terminal region in the t-SNARE comple

197 -opening rates but also the cooperativity of unfolding of the different secondary structural segments

198 7) s(-1) Molecular simulations revealed that unfolding of the disulfide bond-lacking variant is initi

199 wo-state transition in which the folding and unfolding of the first G3T monomer is rate-limiting for

200 We induce targeted mechanical unfolding of the G-quadruplex while leaving the nanocage

201 n of the PC4-G4DNA complex did not result in unfolding of the G4DNA structure.

202 ar factors in oocytes and is associated with unfolding of the inhibitory structure that blocks the te

203 was caused by the absence of pupylation and unfolding of the iron storage protein ferritin.

204 sheds light on the origin and developmental unfolding of the link between language and cognition in

205 This tension is resolved by reversible unfolding of the linker, which is a singular mechanism t

206 t bound to APC/C through mediating the local unfolding of the Mad2 C-terminal region.

207 f high-quality data, defined as the complete unfolding of the polyprotein.

208 tion and pro-peptide removal will facilitate unfolding of the pre-insertion strands, allowing them to

209 nsile forces generated by neighboring cells, unfolding of the probes leads to a large increase in the

210 A with engineered variants, we show that the unfolding of the protein in the gas phase involves the d

211 isite branching of polymerization after slow unfolding of the protein, which may be a common feature

212 Thermal unfolding of the T domain, measured by circular dichrois

213 but an increase in hydrophobicity indicated unfolding of the tertiary structure in all samples.

214 the above domains is lost, resulting in the unfolding of the three separate stems.

215 ture of cell surfaces and highlights how the unfolding of these convoluted surfaces contributes to ph

216 in vivo or denaturants in vitro trigger the unfolding of this domain, thereby leaving the cleavage s

217 the favorable energetics of low-temperature unfolding of Trp-cage comes from the hydration of hydrop

218 Free energy profiles corresponding to the unfolding of Trp-cage miniprotein in the presence and ab

219 Through calibrated unfolding of two protein-ligand systems: cAMP-bound regu

220 e present a detailed characterization of the unfolding of yeast mitochondrial cyclophilin (CPR3) indu

221 benzene unit(s) and ending with the complete unfolding on a later time scale.

222 t the dynamics of coupled epidemic processes unfolding on the network.

223 cificity at protein interfaces through local unfolding or conformational change.

224 how that degron placement can change whether unfolding or translocation is rate limiting, and establi

225 SMD recapitulated the TSS unfolding order in the absence of Mg(2+), showed depende

226 Here, we examine the solvent-free unfolding pathway for a group of homologous serum albumi

227 The experimental data reveal the unfolding pathway in unprecedented detail.

228 folding and permitting data fitting based on unfolding pathway models.

229 lfide bond affects the stability and folding/unfolding pathway of DraE.

230 Our findings suggest that the general unfolding pathway of low charge state albumin ions is la

231 at human albumin follows a domain associated unfolding pathway, and we are able to assign each unfold

232 g a complex mechanism in HdeA's acid-induced unfolding pathway, as previously postulated from molecul

233 of FimG is due to a shift in the mechanical unfolding pathway.

234 The different unfolding pathways exhibited by these two types of array

235 mic force microscopy to study the individual unfolding pathways of the human gammaD-crystallin, a mul

236 the experimental observations, thus matching unfolding patterns with structural events.

237 e two ATPase rings and accounts for the high unfolding potential of Hsp104.

238 the solution-phase structural stability and unfolding process of human carbonic anhydrase I (HCA-I).

239 oscopy was used for the determination of the unfolding process, mechanism and extent of peptide aggre

240 energy landscape in the protein folding and unfolding process.

241 Defensin-induced unfolding promoted exposure of hydrophobic groups otherw

242 and activation of ER stress followed by the unfolding protein response (UPR).

243 Inhibition of ER stress and unfolding protein response pathways causes skeletal musc

244 urants are the most commonly used agents for unfolding proteins and are thought to act by better solv

245 sps) prevent the irreversible aggregation of unfolding proteins by integrating into forming aggregate

246 ons were reported for other proteins for the unfolding rate [Formula: see text].

247 oth proteins exhibited similar kinetics, the unfolding rate constant changed upon deletion of the dis

248 med by experimentally determined folding and unfolding rate constants of DraE-sc and a disulfide bond

249 odynamic stability correlates primarily with unfolding rate rather than folding rate.

250 unfolding transition state-dictates the LC's unfolding rate.

251 The unfolding rates correlate well with the thermodynamic st

252 determine the stability and the folding and unfolding rates of 12 alanine-based alpha-helical peptid

253 troscopy allows determination of folding and unfolding rates on much faster time scales, yet even thi

254 bate in the protein-folding field is whether unfolding rates or folding rates correlate to the stabil

255 The unfolding rates varied widely and correlated with thermo

256 ld be primarily caused by differences in the unfolding rates.

257 t kinetic rate constants for the folding and unfolding reactions.

258 ful yet accessible means to characterize the unfolding/refolding dynamics of individual molecules and

259 RBC spectrin cytoskeleton, including domain unfolding/refolding.

260 specificity and the mechanism involved in GQ unfolding remain uncertain.

261 et their role for protein stability, and for unfolding, remains elusive.

262 se, showing a specific and sensitive protein unfolding signature.

263 Under conditions promoting protein unfolding, small heat shock proteins (sHsps) prevent the

264 perones act as a system when they respond to unfolding stress rather than achieving efficient folding

265 tweezers, together with traditional protein unfolding studies to explore how perturbants commonly us

266 The protease is also capable of processively unfolding substrate proteins with substantial thermodyna

267 ctions in concert with the 20S proteasome by unfolding substrates and passing them on for degradation

268 normal VWF enhances the probability of local unfolding that enables the A1 domain to conformationally

269 ty, but use a common mechanism of repetitive unfolding that leads to disrupting GQ structure multiple

270 ectrical connectivity, by simply folding and unfolding the structure.

271 nformational stability of clients by locally unfolding them.

272 tion of the energy landscape and the folding/unfolding thermodynamics of a hyperstable RNA tetraloop

273 metric techniques to investigate the folding/unfolding thermodynamics of two three-way junctions with

274 sed on the induction of myofibrillar protein unfolding thus facilitating the formation of the types o

275 a to donor cells and facilitates spontaneous unfolding, thus minimizing surgical time.

276 e predictions of volume changes upon protein unfolding, thus paving the way to proteome-wide computat

277 garding the sensitivity of collision induced unfolding to changes in protein primary and tertiary str

278 g" analysis of consensus TALE (cTALE) repeat unfolding to quantify intrinsic folding free energies, c

279 hange in solvent accessible surface area for unfolding, to probe the denatured state as a function of

280 are consistent with a two-state, cooperative unfolding transition having a melting temperature of Tm

281 ded in different environments as the thermal unfolding transition is approached.

282 difference between the native state and the unfolding transition state-dictates the LC's unfolding r

283 the interface after which Rsn-2 undergoes an unfolding transition that maintains its secondary struct

284 reflects departure from an ideal cooperative unfolding transition.

285 with nearly an ideal two-state (cooperative) unfolding transition.

286 re modulations while undergoing a continuous unfolding transition.

287 The simulations reveal reversible folding/unfolding transitions of the tetraloop into the canonica

288 sistent with this data, the shape of thermal unfolding transitions varies from asymmetric for PR to n

289 offered insight into the process of cohesin unfolding under force.

290 ipid binding, show increased stability (less unfolding) upon ligand binding.

291 hanical stress, we studied its force-induced unfolding using single molecule atomic force microscopy

292 in light chain kinase at dramatically higher unfolding velocities than in previous AFM studies.

293 the avidin tetramer toward dissociation and unfolding, via both its effects on charge state distribu

294 Binding and unfolding was characterized using several biochemical an

295 The cooperativity of unfolding was measured in the absence or presence of a s

296 Since unfolding was studied in native conditions, the sequence

297 Such a pecking order in unfolding well reflects the hierarchical nature of nucle

298 ome c with cardiolipin, resulting in protein unfolding, where the unfolded form interacts with cardio

299 ere, we use single-molecule chemo-mechanical unfolding, which combines force and urea using the optic

300 e we report that unmasking of cryptotopes by unfolding whole cytomegalovirus (CMV) antigen preparatio