ライフサイエンスコーパス: uniporter

1 lity of the mitochondrial Ca2+ uptake sites (uniporter).

2 a recently identified ion channel called the uniporter.

3 an inner membrane Ca(2+) channel called the uniporter.

4 Ca(2+) affinity of the mitochondrial Ca(2+) uniporter.

5 ane-potential-dependent mechanism called the uniporter.

6 ential component of the mitochondrial Ca(2+) uniporter.

7 n that serves as a putative regulator of the uniporter.

8 e to Ru360, the most potent inhibitor of the uniporter.

9 he R181C variant exclusively functioned as a uniporter.

10 ix calcium through the mitochondrial calcium uniporter.

11 istent with mitochondrial iron uptake by the uniporter.

12 nt source of protons for inactivation of the uniporter.

13 of mPT induction at a site distinct from the uniporter.

14 at the phenomenological level of the Ca(2+) uniporter.

15 rial Ca2+ uptake via a primary effect on the uniporter.

16 ATP-sensitive K(+) channels, or [Ca(2+)](m) uniporter.

17 ly by converting the proton symporter into a uniporter.

18 .5) = 3 microm) via the mitochondrial Ca(2+) uniporter.

19 hrough the potential-dependent mitochondrial uniporter.

20 novel inhibitor of the mitochondrial calcium uniporter.

21 5 behaves as a specific low affinity glucose uniporter.

22 g, is catalyzed by the mitochondrial calcium uniporter.

23 via an inner membrane transporter called the uniporter.

24 lls overexpressing the mitochondrial calcium uniporter.

25 werful in vivo reconstitution system for the uniporter.

26 tion system, we then reconstituted the human uniporter.

27 rane protein 1 and the mitochondrial calcium uniporter.

28 interaction of NLF and mitochondrial Ca(2+) uniporter.

29 matrix after Ca2+ transport through the Ca2+ uniporter.

30 ily to be classified as strict exchangers or uniporters.

31 t amounts of Ca(2+) from the cytosol via the uniporter, a Ca(2+)-selective ion channel in the inner m

32 ta on the kinetics of Ca2+ transport via the uniporter, a mechanistic kinetic model of the uniporter

33 he minimal components sufficient for in vivo uniporter activity are unknown.

34 Blocking mitochondrial Ca(2+) uniporter activity compromises the ability of mitochondr

35 l membrane, we compare mitochondrial calcium uniporter activity in mouse heart, skeletal muscle, live

36 sophila flight muscle, mitochondrial calcium uniporter activity is barely detectable compared with th

37 and flight muscle, low mitochondrial calcium uniporter activity is likely essential to avoid cytosoli

38 Simultaneously, low mitochondrial calcium uniporter activity may also prevent mitochondrial Ca(2+)

39 ial-dependent calcium uptake compatible with uniporter activity, and also that expression of DdMCU co

40 ents sufficient for metazoan and nonmetazoan uniporter activity, and provide valuable insight into th

41 s affected by H2PO4(-) (P(i)), Mg2+, calcium uniporter activity, matrix volume changes, and the bioen

42 expression of MCU and EMRE is sufficient for uniporter activity, whereas expression of MCU alone is i

43 ly lack pharmacological agents for targeting uniporter activity.

44 icient to reconstitute mitochondrial calcium uniporter activity.

45 ane potential-dependent mitochondrial Ca(2+) uniporter and 2) the evolutionarily conserved exchangers

46 occurs via a ruthenium red-sensitive calcium uniporter and a rapid mode of Ca(2+) uptake.

47 says, that Zn2+ is imported through the Ca2+ uniporter and directly targets major enzymes of energy p

48 hondrial matrix Ca(2+), determined by Ca(2+) uniporter and Na(+)/Ca(2+) exchanger activities, regulat

49 del based on known kinetic properties of the uniporter and presumed Mg(2+) inhibition and Pi regulati

50 of Cac requires both the mitochondrial Ca2+ uniporter and the mitochondrial energization that drives

51 to ER solute import during ER transit, while uniporters and cation-coupled transporters carry out exp

52 calization, the TcMCU (mitochondrial calcium uniporter) and TcIP3R (inositol 1,4,5-trisphosphate rece

53 o mitochondria was dependent upon the Ca(2+) uniporter, and the consequent swelling resulted from ope

54 cle cells with Ru360, a mitochondrial Ca(2+) uniporter antagonist, reversed alterations in the plasma

55 of EMRE ensures that all transport-competent uniporters are tightly regulated, responding appropriate

56 he technique that originally established the uniporter as a Ca(2+) channel.

57 dulating agents identified the mitochondrial uniporter as a critical regulatory factor in bortezomib

58 of MICU1, regulator of mitochondrial calcium uniporter, as a key molecule conferring cancer cells wit

59 were abolished by the mitochondrial calcium uniporter blocker Ru360.

60 more sensitive to the mitochondrial Ca(2)(+) uniporter blocker Ru360.

61 d can be prevented by the mitochondrial Ca2+ uniporter blocker Ruthenium 360; and (v) apoptosis invol

62 ngs by treatment with the mitochondrial Ca2+ uniporter blocker Ruthenium Red (10 microM) potentiated

63 lcium chelator BAPTA-AM and the Ca(2+)(mito) uniporter blocker ruthenium red prevented E2-induced cel

64 were inhibited by both ruthenium red, a Ca2+-uniporter blocker, and by high concentrations of EGTA.

65 (f) The Ca2+ uniporter blocker, Ruthenium Red, protects enzyme activi

66 d, an inhibitor of the mitochondrial calcium uniporter, both rescued mutant striatal cells from 3-NP-

67 th the IP3-induced initial activation of the uniporter but inhibited the sustained phase.

68 at which free ATP and free Mg2+ inhibit the uniporter can be distinguished by chymotrypsin treatment

69 ruthenium red-sensitive mitochondrial Ca(2+) uniporter catalyzes Ca(2+) uptake during beat-to-beat tr

70 In its absence, uniporter channel activity was lost despite intact MCU e

71 MCU encodes the pore-forming subunit of the uniporter channel.

72 The mitochondrial Ca(2+) uniporter complex (MCUC) is a multimeric ion channel whi

73 teins that make up the mitochondrial calcium uniporter complex (MCUC) mediating Ca(2+)uptake into the

74 a(2+) signalling is the mitochondrial Ca(2+) uniporter complex (uniplex), an inner membrane Ca(2+) tr

75 pression did not alter mitochondrial calcium uniporter complex component levels.

76 l function, EMRE could paradoxically inhibit uniporter complex formation if expressed in excess.

77 The mitochondrial calcium uniporter complex is essential for calcium (Ca(2+)) upta

78 lts identify MICU2 as a new component of the uniporter complex that may contribute to the tissue-spec

79 ulatory subunit of the mitochondrial calcium uniporter complex.

80 erived fibroblasts, the mitochondrial Ca(2+) uniporter components MCU, MCUR1, and MICU1 remain unalte

81 0, an inhibitor of the mitochondrial calcium uniporter, consistent with mitochondrial iron uptake by

82 low volume of the ER, trace amounts of these uniporters contribute to ER solute import during ER tran

83 ating kinetic models of mitochondrial Ca(2+) uniporter (CU), Na(+)-Ca(2+) exchanger (NCE), and Na(+)-

84 Hence, EMRE is essential for in vivo uniporter current and additionally bridges the calcium-s

85 w a dramatically lower mitochondrial calcium uniporter current density than the other tissues studied

86 oupling protein 3- and mitochondrial calcium uniporter-dependent, but leucine zipper-EF-hand containi

87 These results suggest that the uniporter displays a calmodulin-mediated facilitation.

88 Inhibition of the mitochondrial Ca(2+) uniporter disrupted the rhythmic production and extracel

89 activation and deactivation kinetics of the uniporter during IP3 receptor-mediated Ca2+ mobilization

90 ce of Deltapsim, basal mitochondrial calcium uniporter expression, and mitochondrial Ca(2+) levels, e

91 acrophages utilize the mitochondrial calcium uniporter for profibrotic polarization.

92 elective blocker of the mitochondrial Ca(2+) uniporter) for 30 min prior to propofol treatment restor

93 e Mg(2+) inhibition and Pi regulation of the uniporter function are not well established.

94 picts the inhibitory effect of Mg(2+) on the uniporter function, in which Ca(2+) uptake is hyperbolic

95 nding of the effects of Mg(2+) and Pi on the uniporter function, we developed here a mathematical mod

96 ition mechanism for Mg(2+) inhibition of the uniporter function.

97 iology, the structure and composition of the uniporter functional unit and kinetic mechanisms associa

98 t identification of the mitochondrial Ca(2+) uniporter gene (Mcu/Ccdc109a) has enabled us to address

99 lished, since knockdown of all the candidate uniporter genes inhibit Ca(2+) uptake in imaging assays,

100 The molecular components of the human uniporter holocomplex (uniplex) have been identified rec

101 eve a full molecular characterization of the uniporter holocomplex (uniplex).

102 forming and Ca(2+)-conducting subunit of the uniporter holocomplex, but its primary sequence does not

103 high sensitivity of the mitochondrial Ca(2+) uniporter in neurons to cytosolic Ca(2+).

104 ous theoretical models of mitochondrial Ca2+ uniporter in the literature in that it is thermodynamica

105 ttle is known about the mechanism underlying uniporter inactivation.

106 m red, a blocker of the mitochondrial Ca(2+) uniporter, inhibited mitochondrial Rhod 2 fluorescence t

107 ial Ca(2+)uptake due to mitochondrial Ca(2+) uniporter inhibition (simulating Ru360) or elevated cyto

108 of Ca(2+) entry into the mitochondria by the uniporter inhibitor RU360 or by cyclosporin A significan

109 Application of the mitochondrial uniporter inhibitor Ru360 reduced mitochondrial and cyto

110 23187 in the presence or absence of the Ca2+ uniporter inhibitor ruthenium red (RR).

111 Mtb in presence of the mitochondrial calcium uniporter inhibitor ruthenium red showed increased mitoc

112 RU-360, a mitochondrial-uniporter inhibitor, abrogated mitochondrial Ca2+ accumu

113 entirely inhibited by the mitochondrial Ca2+ uniporter inhibitor, Ru-360, but not influenced by an Na

114 either a mitochondrial uncoupler or a Ca(2+) uniporter inhibitor.

115 and by Ruthenium Red, a mitochondrial Ca(2+)-uniporter inhibitor.

116 ty is sensitive to the mitochondrial calcium uniporter inhibitors Ruthenium Red and Gd(3+), as well a

117 The uniporter inhibitors ruthenium red and Ru360 prevented c

118 The mitochondrial calcium uniporter is a Ca(2+)-activated Ca(2+) channel complex m

119 llowing such entry, the mitochondrial Ca(2+) uniporter is a highly Ca(2+)-selective channel complex e

120 The mitochondrial calcium uniporter is a highly selective calcium channel distribu

121 The mitochondrial uniporter is a highly selective calcium channel in the o

122 The mitochondrial calcium uniporter is a highly selective channel responsible for

123 The uniporter is a multi-subunit Ca(2+)-activated Ca(2+) cha

124 Thus, the mitochondrial Ca2+ uniporter is a newly identified target for viral modific

125 trophysiological studies have shown that the uniporter is an ion channel with remarkably high conduct

126 The uniporter is composed of the pore-forming MCU protein, t

127 d within the context of a model in which the uniporter is considered to be a gated channel that is co

128 equent matrix Ca(2+) reuptake via the Ca(2+) uniporter is estimated to be >100-fold slower than matri

129 niporter, a mechanistic kinetic model of the uniporter is introduced.

130 rocess of Ca(2+) uptake by the mitochondrial uniporter is itself regulated by Ca(2+) in a temporally

131 Transport by the uniporter is membrane potential dependent and sensitive

132 ow that in addition to divalent cations, the uniporter is regulated by external adenine nucleotides a

133 y to demonstrate that the mitochondrial Ca2+ uniporter is strongly inhibited by external EGTA plus fr

134 The uniporter is subject to inactivation, whereby a sustaine

135 Ca2+ transport through mitochondrial Ca2+ uniporter is the primary Ca2+ uptake mechanism in respir

136 -induced increase in the permeability of the uniporter lasted longer than the Ca2+ signal.

137 to constituents of the mitochondrial Ca(2+) uniporter machinery in mammals.

138 e valuable insight into the evolution of the uniporter machinery.

139 gi, and show that it has a highly simplified uniporter machinery.

140 The analyses indicate that the uniporter may have been an early feature of mitochondria

141 The Ca(2+) uniporter MCU mediates Ca(2+) uptake, whereas NCLX (mito

142 ) accumulation (via the mitochondrial Ca(2+) uniporter MCU) in CA1 but not in CA3 neurons and was mar

143 We find that the mitochondrial calcium uniporter MCU-1 is essential for rapid mitochondrial Ca(

144 2+) influx through the mitochondrial calcium uniporter (MCU) and a rise in matrix [Ca(2+) ].

145 Mitochondria take up Ca2+ via the Ca2+ uniporter (MCU) and extrude it through the mitochondrial

146 r identification of the mitochondrial Ca(2+) uniporter (MCU) and of unique targeted Ca(2+) probes to

147 al upregulation of the mitochondrial calcium uniporter (MCU) and the mitochondrial calcium uptake 1 p

148 ough the inner membrane mitochondrial Ca(2+) uniporter (MCU) are not known.

149 transients, as did the mitochondrial Ca(2+) uniporter (mCU) blocker, Ru360.

150 o the recently proposed mitochondrial Ca(2+) uniporter (MCU) candidate.

151 termine how changes in mitochondrial calcium uniporter (MCU) complex (MCUC) function influence mitoch

152 ake is mediated by the Mitochondrial Calcium Uniporter (MCU) complex, a macromolecular structure that

153 rongly dependent on the mitochondrial Ca(2+) uniporter (MCU) complex, has a series of key roles in ph

154 Additionally, mitochondrial Ca(2+) uniporter (MCU) currents were lower in KO myocytes, indi

155 ked by knockdown of the mitochondrial Ca(2+) uniporter (MCU) expression.

156 The mitochondrial calcium uniporter (MCU) facilitates calcium entry into the mitoc

157 iated knockdown of the mitochondrial calcium uniporter (MCU) gene reduces mitochondrial Ca(2+) curren

158 cium homeostasis and the Mitochondria Cacium Uniporter (MCU) in cell migration were recently highligh

159 The mitochondrial calcium uniporter (MCU) is a highly selective ion channel that t

160 he recently discovered Mitochondrial Calcium Uniporter (MCU) is controlled by its gatekeeper Mitochon

161 Mitochondrial calcium uniporter (MCU) is the pore-forming and Ca(2+)-conductin

162 take is undertaken by the mitochondrial Ca2+ uniporter (MCU) located in the organelle's inner membran

163 nt protein kinase II, a mitochondrial Ca(2+) uniporter (MCU) regulator, also prevented MPTP formation

164 N or C terminus of the mitochondrial calcium uniporter (MCU), a recently identified channel whose top

165 =13), an inhibitor of the mitochondrial Ca2+ uniporter (mCU), and (3) 2-aminoethoxydiphenylborane (10

166 cium uptake, through a mitochondrial calcium uniporter (MCU), is important not only for the regulatio

167 ane-spanning subunits--mitochondrial calcium uniporter (MCU), its paralog MCUb, and essential MCU reg

168 a(2+) uptake 1 (MICU1), mitochondrial Ca(2+) uniporter (MCU), uncoupling protein 2 (UCP2), and leucin

169 Mitochondrial calcium uniporter (MCU), which is the core channel subunit of MC

170 ase properties through Mitochondrial Calcium Uniporter (MCU)-dependent Ca2+ clearance.

171 ncode homologues of the mitochondrial Ca(2+) uniporter (MCU).

172 ected by silencing the mitochondrial calcium uniporter (MCU).

173 naptic calcium via the mitochondrial calcium uniporter (MCU).

174 gative (DN) form of the mitochondrial Ca(2+) uniporter (MCU).

175 d by the inhibition of mitochondrial calcium uniporter (MCU).

176 e pore-forming subunit mitochondrial calcium uniporter (MCU).

177 SLC25A23 interacts with mitochondrial Ca(2+) uniporter (MCU; CCDC109A) and MICU1 (CBARA1) while also

178 09A, that we now call 'mitochondrial calcium uniporter' (MCU).

179 do-steady-state influx rates of Ca2+ via the uniporter measured under a wide range of experimental co

180 This uniporter-mediated mitochondrial Ca(2+) transport is als

181 embrane protein 1- and mitochondrial calcium uniporter-mediated, but uncoupling protein 3-independent

182 a(2+) entry through the mitochondrial Ca(2+) uniporter modulates mitochondrial transport, and mitocho

183 he inner mitochondrial membrane (IMM) Ca(2+) uniporter (mtCU).

184 uptake pathway, which is neither the Ca(2+) uniporter nor the rapid mode of Ca(2+) uptake.

185 achomatis Npt1 (Npt1(Ct)) and the nucleotide uniporter Npt2(Ct), which transports GTP, UTP, CTP, and

186 ate that SWEET17 functions as a Fru-specific uniporter on the root tonoplast.

187 g either mitochondrial Ca(2+) uptake via the uniporter or Ca(2+) release via the mitochondrial Na(+)/

188 e irreconcilable, and any passive asymmetric uniporter or cotransporter model system, e.g., Na-glucos

189 nsport in cells that appear not to have urea uniporters or channels.

190 that HKT family members are sodium-selective uniporters or sodium-potassium symporters is widely held

191 60, an inhibitor of the mitochondrial Ca(2+) uniporter, or with EGTA acetoxymethyl ester, but not wit

192 The uniporter passes Ca2+ down the electrochemical gradient

193 y KCl or carbachol, indicating that the Ca2+ uniporter pathway played a role in the first, but not in

194 ffect evoked a large further increase in the uniporter permeability.

195 Thus, the uniporter plays a key role in regulating mitochondrial C

196 MICU1 interacts with the uniporter pore-forming subunit MCU and sets a Ca(2+) thr

197 The molecular nature of the uniporter remained unknown for decades.

198 ial Na+/Ca2+ exchanger or by reversal of the uniporter responsible for energy-dependent Ca2+ uptake.

199 on, an inhibitor of the mitochondrial Ca(2+) uniporter (RU-360) attenuated mitochondrial Ca(2+) uptak

200 tor of the mitochondrial Ca(2+) (and Fe(2+)) uniporter, Ru360, protected against PDT plus bafilomycin

201 Although the uniporter's biophysical properties have been studied ext

202 cterize the phylogenomic distribution of the uniporter's membrane-spanning pore subunit (MCU) and reg

203 The mitochondrial calcium uniporter shapes cytosolic Ca(2+) signals, controls mito

204 Protease-resistant EMRE mutants produce uniporter subcomplexes that induce constitutive Ca(2+) l

205 er membrane protein EMRE was identified as a uniporter subunit absolutely required for Ca(2+) permeat

206 The results highlight the dynamic nature of uniporter subunit assembly, which must be tightly regula

207 e findings support the idea that a conserved uniporter system, with composition and regulation distin

208 the Trypanosoma brucei mitochondrial calcium uniporter (TbMCU) is essential for the regulation of mit

209 l is extended from our previous model of the uniporter that is based on a multistate catalytic bindin

210 rimarily by two major transporters: a Ca(2+) uniporter that mediates Ca(2+) uptake and a Na(+)/Ca(2+)

211 m uptake occurs through a channel called the uniporter that resides in the inner mitochondrial membra

212 Most insect cell membranes seem to contain uniporters that facilitate the diffusion of amino acids

213 t identification of the mitochondrial Ca(2+) uniporter, the channel allowing rapid Ca(2+) accumulatio

214 on and silencing of the mitochondrial Ca(2+) uniporter, the major mitochondrial Ca(2+) uptake protein

215 Ca(2+) mediated by the mitochondrial calcium uniporter through a process involving the translocation

216 ease sites, because the mitochondrial Ca(2+) uniporter was homogeneously distributed, and elevated [C

217 Because RuR inhibits mitochondrial Ca(2+) uniporter, we tested whether the NLF acts via the mechan

218 GLT1 and functions as a low affinity glucose uniporter, were expressed as individual proteins in Xeno

219 ochondrial Ca (mCa) overload through the mCa uniporter, which can ultimately lead to apoptosis and gr

220 d into respiring mitochondria via the Ca(2+) uniporter, which is known to be inhibited by Mg(2+).

221 ort by H322N mutant; how H322 mutants become uniporters; why exchanging Lys-319 with Asp-240 paradoxi

222 re FCCP or blocking the mitochondrial Ca(2+) uniporter with Ru360 as well as blocking the respiratory

223 yphenylhydrazone or inhibition of the Ca(2+) uniporter with Ru360 prevented rapid onset of the swelli

224 t not by inhibiting the mitochondrial Ca(2+) uniporter with Ru360.