ライフサイエンスコーパス: unnatural base pair

1 rs two additional letters that form a third, unnatural base pair.

2 the effort to develop stable and replicable unnatural base pairs.

3 polymerase-mediated replication of the same unnatural base pairs.

4 a large number of predominantly hydrophobic unnatural base pairs.

5 These marker nucleotides constitute an unnatural base pair, allowing large quantities of marked

6 ch should aid in further optimization of the unnatural base pair and possibly in the design of additi

7 e of extension of primers terminating at the unnatural base pair and, interestingly, requires that th

8 ase I to synthesize and extend the different unnatural base pairs and mispairs under steady-state con

9 ation properties of many previously examined unnatural base pairs and should help design unnatural ba

10 nus that seem to limit replication of larger unnatural base pairs, and they therefore represent a pro

11 han dMMO2 and that overall the corresponding unnatural base pairs are generally replicated with highe

12 Several unnatural base pairs are identified that are both reason

13 The differences between these unnatural base pairs are manifest only in the polymerase

14 Surprisingly, several of the unnatural base pairs are virtually as stable as a natura

15 terize a library of templates containing the unnatural base pair as a function of amplification.

16 The most promising unnatural base pair candidate of this series is the 3-fl

17 We show here that both of the unnatural base pairs d5SICS:dMMO2 and d5SICS:dNaM are se

18 s them to replicate a plasmid containing the unnatural base pair dNaM-dTPT3.

19 The unnatural base pairs do not appear to induce major struc

20 a wide variety of predominantly hydrophobic unnatural base pairs exemplified by d5SICS-dMMO2 and d5S

21 netic alphabet, we have developed a class of unnatural base pairs, exemplified by d5SICS-dMMO2 and d5

22 We recently developed a class of candidate unnatural base pairs, exemplified by the pair formed bet

23 ts significant progress toward developing an unnatural base pair for the in vivo expansion of an orga

24 timization that led to identification of the unnatural base pair formed between the nucleotides dMMO2

25 Previously, the unnatural base pairs formed between d5SICS and either dN

26 orted the synthesis and thermal stability of unnatural base pairs formed between nucleotides bearing

27 We now report the synthesis and stability of unnatural base pairs formed between simple phenyl rings

28 its expansion by the development of a third, unnatural base pair has emerged as a central goal of che

29 A variety of unnatural base pairs have been shown to be formed effici

30 While a variety of unnatural base pairs have been shown to be substrates fo

31 ntaining 2'-O-methyl-modified nucleotides or unnatural base pairs, have been evolved.

32 aM is one of the most efficiently replicated unnatural base pairs identified to date, but its pairing

33 ino acid, and efficient participation of the unnatural base pair in decoding at the ribosome.

34 ted that this method can be used to discover unnatural base pairs in DNA with a detection threshold o

35 mation requires in vivo transcription of the unnatural base pair into mRNA and tRNA, aminoacylation o

36 We found that the unnatural base pair is efficiently replicated with high

37 mistry complexes in which the already formed unnatural base pair is positioned at the postinsertion s

38 This unnatural base pair is replicated by the Klenow fragment

39 Moreover, polymerase recognition of the unnatural base pairs is found to be very sensitive to bo

40 zed, the detailed recognition of most of the unnatural base pairs is generally polymerase dependent.

41 ell as continued primer extension beyond the unnatural base pair, is sensitive to the specific methyl

42 ining either the d5SICS-dNaM or d5SICS-dMMO2 unnatural base pair may be amplified by PCR with fidelit

43 strate that the determinants of a functional unnatural base pair may be designed into predominantly h

44 Importantly, the results reveal that the unnatural base pairs may function within an expanded gen

45 pand the genetic alphabet, by addition of an unnatural base pair, promise to expand the biotechnologi

46 Not only are these unnatural base pairs promising for different application

47 While we find that some aspects of unnatural base pair recognition are conserved among the

48 a allow us to propose a generalized model of unnatural base pair replication, which should aid in fur

49 unnatural base pairs and should help design unnatural base pairs that are better replicated.

50 air and possibly in the design of additional unnatural base pairs that are replicated with truly natu

51 As part of an effort to develop unnatural base pairs that are stable and replicable in D

52 ply that further modifications may result in unnatural base pairs that can be both efficiently synthe

53 imize the slowest step of replication of the unnatural base pair, the insertion of dMMO2 opposite d5S

54 es the pairing nucleotides of the developing unnatural base pair to adopt a planar Watson-Crick-like

55 We have developed an unnatural base pair (UBP) and a semisynthetic organism (

56 two synthetic nucleotides that form a third, unnatural base pair (UBP) have recently yielded three pr

57 increased information, we have developed the unnatural base pairs (UBPs) dNaM and d5SICS or dTPT3 (dN

58 We have developed a family of unnatural base pairs (UBPs), which rely on hydrophobic a

59 lphabet has been expanded to include several unnatural base pairs (UBPs).

60 f an effort to develop stable and replicable unnatural base pairs, we have evaluated a large number o

61 We synthesized a panel of unnatural base pairs whose pairing depends on hydrophobi

62 base pairs, and the development of a third, unnatural base pair would increase the genetic and chemi