ライフサイエンスコーパス: unspliced

1 d by ICP27 transactivation was predominantly unspliced.

2 iptional analysis of this mutant showed that unspliced 19S RNA was not transported and remained withi

3 can act to increase the ratio of spliced to unspliced AAV RNA when they are targeted to the transcri

4 lt in enhancement of the ratio of spliced to unspliced AAV RNA.

5 ns: CXCR4-A and CXCR4-B, corresponding to an unspliced and a spliced mRNA, respectively.

6 oximately half of the RNA transcripts remain unspliced and either are used to encode Gag and Gag-Pol

7 ex with a 351 nucleotide sequence present in unspliced and incompletely spliced human immunodeficienc

8 for efficient cytoplasmic expression of the unspliced and incompletely spliced viral mRNA transcript

9 to induce the cytoplasmic expression of the unspliced and incompletely spliced viral RNAs encoding t

10 approximately half of the viral RNA remains unspliced and is used as genomic RNA and as mRNA for the

11 Both unspliced and multiply spliced forms were found.

12 sequence evolution and the concentrations of unspliced and multiply spliced mRNA provided evidence of

13 Patient-matched PCR for unspliced and multiply spliced viral RNAs combined with

14 Here, we show that unspliced and partially spliced forms of the MATa1 mRNA

15 ntial for the nucleocytoplasmic transport of unspliced and partially spliced HIV mRNAs containing the

16 elements (RREs) is required for transport of unspliced and partially spliced human immunodeficiency v

17 IV-1 Rev protein activates nuclear export of unspliced and partially spliced viral RNA transcripts, w

18 complex signals nucleocytoplasmic export of unspliced and partially spliced viral RNA.

19 ponse element (RRE) is a 351-base element in unspliced and partially spliced viral RNA; binding of th

20 cialized nuclear export pathway to transport unspliced and partially spliced viral transcripts to the

21 The UL37 promoter drives production of an unspliced and several alternatively spliced RNAs.

22 (RRE) is essential for the nuclear export of unspliced and singly spliced human immunodeficiency viru

23 dramatically decreased accumulation of HIV-1 unspliced and singly spliced RNAs and altered splice sit

24 Nuclear export of unspliced and singly spliced viral mRNA is a critical st

25 therefore indispensable for the transport of unspliced and singly spliced viral transcripts.

26 HIV replication requires nuclear export of unspliced and singly spliced viral transcripts.

27 plication by mediating the nuclear export of unspliced and singly-spliced viral mRNAs.

28 A single transcript in its unspliced and spliced forms directs the synthesis of all

29 he rapid and transient induction of both the unspliced and spliced forms of the UPR gene bZIP60.

30 died the in vitro dimerization properties of unspliced and spliced HIV-2 RNA.

31 xpressed in skeletal muscle and exists as in unspliced and spliced isoforms, and its 5' end overlaps

32 The unspliced and spliced TgMTP1 variants differ within a hi

33 These unspliced and spliced transcripts and putative proteins

34 Consistent with this prediction, both unspliced and spliced UL29/28 transcript was present in

35 Analysis of the levels of unspliced and spliced viral RNA produced by the parental

36 JSRV encodes unspliced and spliced viral RNAs, among which unspliced

37 in B2, OcaB (BOB1, Pou2af1), and XBP1 mRNAs, unspliced and spliced, are severely reduced in ELL2-defi

38 HBV DNA quantification with S (unspliced) and X (total DNA) regions provided different

39 aining the proper equilibrium among spliced, unspliced, and partially spliced isoforms is essential f

40 rrence of variants and molecules that remain unspliced at nearby exon-intron boundaries.

41 Rather, it retains the unspliced beta-globin mRNA in the nucleus.

42 riments also showed that p68 interacted with unspliced but not spliced mRNA in vivo.

43 gle intron; in maize leaves both spliced and unspliced Bz2 transcripts are usually present and are pr

44 es a significant increase in cell-associated unspliced (CA-US) HIV-1 RNA from CD4(+) T cells.

45 r levels of HIV DNA (P< 0.001) and increased unspliced cellular HIV RNA transcription (P= 0.010).

46 een levels of virions in the supernatant and unspliced cellular HIV-1 RNA following anti-CD3/CD28 tre

47 ic acid (SAHA; vorinostat) show increases in unspliced cellular HIV-1 RNA levels in resting CD4(+) T

48 ns, and the exonic structures of spliced and unspliced coding and noncoding RNAs.

49 ion is attenuated, Gag binding promoted, and unspliced dimeric genomes selected, by the RNA conformer

50 ments that direct selective packaging of the unspliced, dimeric viral RNA into assembling particles.

51 itive sequences within an Epstein-Barr virus unspliced early gene, LF3, which is associated with the

52 omitant with an increase in the abundance of unspliced early transcripts.

53 ture at 5' splice sites that correlates with unspliced events.

54 ich exists in the transcriptionally inactive unspliced form [XBP1(U)] and the spliced active form [XB

55 Herein we identified unspliced forms of LDH and ENO transcripts produced duri

56 lated by the balance between its spliced and unspliced forms.

57 ization capture strategy for purification of unspliced full-length HIV RNA-protein complexes preserve

58 er and results in increased transcription of unspliced gag and spliced nef viral RNA.

59 these mutants, we quantitated the levels of unspliced gag and spliced pol mRNAs using a real-time PC

60 educed as much as 30-fold, whereas levels of unspliced gag RNA were not affected.

61 little effect on cytoplasmic accumulation of unspliced gag-pol RNA despite complete elimination of de

62 protein production and the nuclear export of unspliced gag-pol RNA.

63 SRp40 increased nuclear localization of the unspliced Gag/Pol mRNA, while the same factors increased

64 of HSV-2 ICP34.5 can be translated from the unspliced gamma34.5 mRNA.

65 RP2 causes a defective spliceosome to retain unspliced gene transcripts in the nuclei of human cells.

66 press Gag and Pol proteins by translation of unspliced genome-length viral RNA.

67 of spliced env mRNA and highly enriched for unspliced genome.

68 green fluorescent protein (EGFP) within its unspliced genomic context.

69 luding alternative splicing and packaging of unspliced genomic RNA into virions.

70 Full-length unspliced genomic RNA plays critical roles in HIV replic

71 cy virus type 1, synthesize Gag and Pol from unspliced genomic RNA.

72 ete foci of higher-order oligomers, to which unspliced HAC1 mRNA is recruited by means of a conserved

73 ges are detected in the levels of spliced or unspliced HAC1 mRNA or in the stability of Hac1p.

74 sence of ER stress, ribosomes are stalled on unspliced HAC1 mRNA.

75 tion represses translation initiation on the unspliced HAC1 mRNA.

76 ction site that derepress translation of the unspliced HAC1 mRNA.

77 Whereas in yeast translation of unspliced Hac1p is blocked, mammalian Xbp-1u is synthesi

78 elimination of unspliced Xbp-1 (Xbp-1u) and unspliced Hac1p, respectively.

79 An unspliced HBV genome was dominant, but 100% of strains w

80 Platelets contain unspliced heteronuclear IL-1beta RNA, which is rapidly s

81 ram resulted in increases in cell-associated unspliced HIV RNA at all doses, consistent with activati

82 e median maximum increase in cell-associated unspliced HIV RNA during panobinostat treatment was 3.5-

83 estimated fold increases in cell-associated unspliced HIV RNA from baseline were 1.7 (95% CI 1.3-2.2

84 imary endpoint was change in cell-associated unspliced HIV RNA in CD4 cells.

85 ne proteins were identified to interact with unspliced HIV RNA including Rev and Gag/Gag-Pol, 24 host

86 The level of cell-associated unspliced HIV RNA increased significantly at all timepoi

87 HIV DNA, 2-LTR circles, and cell-associated unspliced HIV RNA were quantified.

88 was change from baseline of cell-associated unspliced HIV RNA.

89 y attenuates the translational output of the unspliced HIV-1 gag mRNA, and possibly all HIV-1 transcr

90 that nuclear Naf1 promoted nuclear export of unspliced HIV-1 gag mRNA, leading to increased Gag produ

91 t nuclear export of incompletely spliced and unspliced HIV-1 mRNA transcripts, which is achieved by R

92 er to allow the accumulation of vif mRNA and unspliced HIV-1 mRNA, compatible with optimal virus repl

93 ear export and cytoplasmic expression of the unspliced HIV-1 mRNAs that encode the viral Gag proteins

94 n(s) that directs cytoplasmic utilization of unspliced HIV-1 reporter RNA.

95 Only very low levels of unspliced HIV-1 RNA ( approximately 50 copies/10(6) rest

96 t (Psi) within the 5' untranslated region of unspliced HIV-1 RNA genome.

97 steady-state levels of multiply spliced and unspliced HIV-1 RNA prior to cellular activation suggest

98 Nuclear export of partially spliced or unspliced HIV-1 RNA transcripts requires binding of the

99 peckles, a structure implicated in retaining unspliced HIV-1 transcripts for either Rev-mediated nucl

100 ve" proviruses capable of transcribing novel unspliced HIV-RNA (usHIV-RNA) species in patients at all

101 Although unspliced host messenger RNAs are rarely exported from t

102 responsive element-independent expression of unspliced human immunodeficiency virus type 1 (HIV-1) ga

103 Full-length unspliced human immunodeficiency virus type 1 (HIV-1) RN

104 ICP34.5beta is translated from unspliced ICP34.5 mRNA, with the retained intron introdu

105 ed population and a population consisting of unspliced IEX-1 RNA.

106 es of the presence of either a spliced or an unspliced intron in a rRNA for ribosome assembly and pac

107 We have previously demonstrated that unspliced intron-containing CTE RNA is efficiently expor

108 These aberrations accompany increased unspliced (intron-retained) and decreased spliced mRNA o

109 ent (CTE), an element that enables export of unspliced, intron-containing mRNA.

110 ated nascent pre-mRNA (CA-RNA) contains many unspliced introns and m(6)A in exons but very rarely in

111 their genomic and mRNAs contain one or more unspliced introns.

112 dditional exons and alternatively spliced or unspliced introns.

113 1ot1 is transcribed by RNA polymerase II, is unspliced, is relatively stable and is localised in the

114 e UPR is inactive, HAC1 mRNA is stored as an unspliced isoform in the cytoplasm and no Hac1 protein i

115 with Rej deleted showed normal transport of unspliced JSRV RNA to the cytoplasm; however, in 293T ce

116 The unspliced kay(sro) transcript has a lower abundance than

117 s) 71, 72 and 73 proteins as well as a novel unspliced KSHV mRNA that encodes only ORF72 and ORF71.

118 When relative amounts of the spliced and unspliced LAT within the brain, liver, kidney, spinal co

119 slational efficiency compared to that of the unspliced leader.

120 Unspliced LEF1 NAT interacts with LEF1 promoter and faci

121 ts influence the position of genes and their unspliced length.

122 t machinery or how the intron-containing but unspliced M1 mRNA bypasses the normal quality-control ch

123 KL is produced by cytoplasmic translation of unspliced M1 mRNA initiating at CUG codons within the +1

124 The unspliced M1 mRNA is translated into the matrix M1 prote

125 The unspliced M1 mRNA is translated into the matrix M1 prote

126 n, we found that cytoplasmic accumulation of unspliced M1 mRNA was inefficient in the absence of NS1,

127 lthough intron retention events (potentially unspliced messages) had been seen for 36% of the genes o

128 vide a means of estimating the proportion of unspliced MoMLV RNA that serves as genomic RNA.

129 two proteins: the M1 protein translated from unspliced mRNA and the M2 protein produced by mRNA splic

130 comparison, the protein translated from the unspliced mRNA contains a transmembrane domain, localize

131 Segment 7 produces two major transcripts: an unspliced mRNA that encodes the M1 matrix protein and a

132 cripts and effect export from the nucleus of unspliced mRNA, thereby allowing the synthesis of struct

133 This isoform is shown to represent intron 0 unspliced mRNA, whereas the smaller transcript represent

134 runcated D1 (exons 1-4) polypeptide from the unspliced mRNA.

135 ranscripts, but also produced high levels of unspliced mRNA.

136 fficient polyadenylation of both spliced and unspliced mRNAs (encoding a putative 10-kDa protein, ana

137 proviruses or reporter gene expression from unspliced mRNAs and allowed detection of a 33-kDa protei

138 ning complexes that contain both spliced and unspliced mRNAs of housekeeping genes.

139 anslated several times more efficiently than unspliced mRNAs that have the same sequence but lack EJC

140 oviral replication requires both spliced and unspliced mRNAs.

141 ntially higher levels from spliced than from unspliced mRNAs.

142 pies of Vhs from infecting virions than were unspliced mRNAs.

143 However, unspliced nascent transcripts were not detected.

144 ing leads to a more dramatic accumulation of unspliced nascent transcripts.

145 scription, as assessed by the measurement of unspliced, nascent, heterogeneous nuclear RNA, and treat

146 of LEF1 NAT in trans prevents the action of unspliced NAT by competing for interaction with the prom

147 Contrarily to the spliced NAT, this unspliced NAT down-regulates the main LEF1 promoter acti

148 the mRNA encoding Orb2A protein exists in an unspliced non-protein-coding form.

149 extent, the transcripts produced tend to be unspliced, non-polyadenylated and expressed at levels 10

150 of miR-155 is likely a transient spliced or unspliced nuclear BIC transcript rather than accumulated

151 ionally and allows the cytoplasmic export of unspliced or incompletely spliced viral mRNAs carrying g

152 ncoded by P9-generated mRNAs that are either unspliced or spliced within the rep gene region, respect

153 We posit that pasilla-mediated processing of unspliced Orb2A mRNA integrates experience and internal

154 We have mapped the 5' and 3' ends of the unspliced Orf49 transcript, which encodes a 30-kDa prote

155 of Rep 1 was initiated from the first AUG in unspliced P9-generated mRNA; however, this AUG was bypas

156 M-1 is also used to export Rev/RRE-dependent unspliced/ partially spliced HIV-1 RNAs.

157 TMG capping of unspliced/partially spliced HIV-1 RNAs represents a new

158 o1 mutants, ycf3-intron 2 remains completely unspliced, petD intron splicing is strongly reduced, and

159 the simultaneous cytoplasmic accumulation of unspliced (pgRNA) and spliced RNA was not known.

160 mRNA splicing, resulting in accumulation of unspliced pre-mRNA and alternatively spliced mRNA.

161 While these mutants also accumulate unspliced pre-mRNA at the restrictive temperature, the m

162 ination codons, but the role of NMD on yeast unspliced pre-mRNA degradation is controversial.

163 t associates with spliced mRNA, but not with unspliced pre-mRNA in vitro.

164 ignals, surprisingly, apparently full-length unspliced pre-mRNA persisted for several hours in both c

165 cised, lariat form of the intron, as well as unspliced pre-mRNA, suggesting a role for Cwc23 in splic

166 4 and spt5 mutations lead to accumulation of unspliced pre-mRNA.

167 ethal Prp22 mutants leads to accumulation of unspliced pre-mRNAs and excised introns in vivo.

168 nd RPL18A transcripts trigger degradation of unspliced pre-mRNAs and lariat introns and can control t

169 wn in HeLa cells, and identified accumulated unspliced pre-mRNAs by genomic tiling microarrays.

170 MP components led to further accumulation of unspliced pre-mRNAs even in a yeast strain defective in

171 gs with Mlp1, both Esc1 and Ulp1 help retain unspliced pre-mRNAs in the nucleus.

172 scripts and promote the nuclear retention of unspliced pre-mRNAs in yeast.

173 Unspliced pre-mRNAs were also identified as targets for

174 fic cell death and a significant increase in unspliced pre-mRNAs.

175 nd nucleolar RNAs and in the surveillance of unspliced pre-mRNAs.

176 (iii) The unspliced pre-RNA form containing the IEX-1 intron seque

177 ranscripts and, less efficiently, with other unspliced pre-tRNA intermediates but not mature tRNAs.

178 p leads to strong accumulation of all tested unspliced pre-tRNA species, as well as accumulation of 5

179 remature processing of the 5' and 3' ends of unspliced pre-tRNA.

180 epletion of mature tRNAs and accumulation of unspliced pre-tRNAs.

181 ng a 1.2-kbp RT-PCR product) arising from an unspliced precursor likely contributed, albeit to a less

182 yssi can be overexpressed and purified as an unspliced precursor, which allows for a detailed in vitr

183 essed in Escherichia coli and purified as an unspliced precursor.

184 In the absence of functional NMD, unspliced precursors accumulate in the cytoplasm, possib

185 e site mutation in RPS10B, and limits RPS29B unspliced precursors accumulation during amino acid star

186 embrane protein 2A (LMP2A) transcripts whose unspliced precursors cross joined TRs.

187 of unspliced RPS22B pre-mRNAs, degrades most unspliced precursors generated by a 5' splice site mutat

188 of 8-mers in internal noncoding exons versus unspliced pseudo exons and 5' untranslated regions (5' u

189 NMD is capable of targeting both spliced and unspliced PTC-containing mRNAs.

190 ar factor Upf1 are required for the decay of unspliced, PTC-bearing RSV RNA by the NMD pathway.

191 were transfected with plasmids harboring an unspliced renilla luciferase (RLuc) reporter mRNA or RLu

192 ts, NXF1:NXT1 also facilitates the export of unspliced retroviral genomic RNA from simple type-D retr

193 Despite the presence of a 5- to 7-kb 3' UTR, unspliced retroviral RNA escapes this degradation.

194 ially tethering various EJC components to an unspliced RLuc transcript.

195 'UTR conformation on ribosome loading to HIV unspliced RNA and rate of Gag polypeptide synthesis was

196 wo distinct signals derived from spliced and unspliced RNA are measured, providing the basis for a ro

197 WT1(+KTS) expression increases the levels of unspliced RNA containing a CTE and specifically promotes

198 G8 also enhanced polyribosome association of unspliced RNA containing a CTE.

199 9G8 was shown to enhance expression of unspliced RNA containing either the Mason-Pfizer monkey

200 oteins which favors production of the UL37x1 unspliced RNA during HCMV infection at the posttranscrip

201 nspliced and spliced viral RNAs, among which unspliced RNA encodes Gag and Pol proteins and a singly

202 gned microarrays to distinguish spliced from unspliced RNA for each intron-containing yeast gene and

203 arcoma virus (RSV) requires large amounts of unspliced RNA for replication.

204 virus selectively packages two copies of its unspliced RNA genome, both of which are utilized for str

205 jRE did not affect the levels of cytoplasmic unspliced RNA in 293 cells, although the unspliced RNA s

206 d nuclear export/accumulation of cytoplasmic unspliced RNA in 293T cells, similarly to other complex

207 NRS serves to enhance polyadenylation of RSV unspliced RNA in a process analogous to the stimulation

208 ar RNA revealed a major fraction of nascent, unspliced RNA in contrast to results obtained from purif

209 integrated DNA as well as multi-spliced and unspliced RNA in divergent proportions.

210 rates that Tap and NXT efficiently shift the unspliced RNA into polyribosomal fractions.

211 The UL37 exon 1 (UL37x1) unspliced RNA is abundant from IE to late times of HCMV

212 Cell-associated HIV unspliced RNA is an important marker of the viral reserv

213 The unspliced RNA molecules are selected for encapsidation f

214 own of MCM7 or SF3B3 significantly increased unspliced RNA of epidermal growth factor receptor, plate

215 tors further favors production of the UL37x1 unspliced RNA over that of the spliced RNAs.

216 Production of the UL37x1 unspliced RNA requires polyadenylation (PA) at nucleotid

217 ere organization, spindle pole assembly, and unspliced RNA retention.

218 mic unspliced RNA in 293 cells, although the unspliced RNA showed partial degradation, perhaps due to

219 egulated RNA processing with accumulation of unspliced RNA species in AD, including myc box-dependent

220 infrequently detected in gut, and ratios of unspliced RNA to DNA were lower in the colon and rectum

221 e cells were not dormant but were generating unspliced RNA transcripts before treatment was interrupt

222 ytoplasmic accumulations of both spliced and unspliced RNA transcripts of XMRV and MLV, resulting in

223 leocytoplasmic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of

224 mportantly, lower viral transcription (HIV-1 unspliced RNA) and enhanced immune preservation (CD4/CD8

225 le-cell visualization of HIV (a) spliced and unspliced RNA, (b) cytoplasmic and nuclear DNA, and (c)

226 , Rev and Tat spliced RNAs exceeded those of unspliced RNA.

227 through their common effects on spliced and unspliced RNA.

228 mRNA to the total expression of spliced and unspliced RNA.

229 ions between cellular proteins and the viral unspliced RNA.

230 , stalled spliceosomes are disassembled, and unspliced RNAs are released.

231 They also use unspliced RNAs as mRNAs to produce the gag and pol gene

232 Yeast prp2 mutants accumulate unspliced RNAs from the vast majority of intron-containi

233 Retroviruses package full-length, unspliced RNAs into progeny virions as dimerized RNA gen

234 n of gene products from both the spliced and unspliced RNAs is essential, as cells expressing only on

235 Retroviral genomes consist of two unspliced RNAs linked noncovalently in a dimer.

236 The unspliced RNAs were retained in the nucleus, and block o

237 ctional, exosome-sensitive, relatively short unspliced RNAs, the generation of which is strongly rela

238 trategy for correctly sorting spliced versus unspliced RNAs.

239 ntron splicing regulation in vivo, levels of unspliced rnp-4f mRNA in dADAR mutant were compared to w

240 The results show that during embryogenesis unspliced rnp-4f mRNA levels fall by up to 85% in the mu

241 nt RNase III-mediated nuclear degradation of unspliced RPS22B pre-mRNAs, degrades most unspliced prec

242 fca mutations increased levels of unspliced sense FLC transcript, altered processing of an

243 SIV inoculation and quantified the levels of unspliced SIV RNA and spliced SIV RNA in tissue lysates

244 The nearly equal abundance of spliced and unspliced species leaves open possible roles for both is

245 P-1 species and simultaneously stabilize the unspliced species that acts as a dominant negative.

246 If this intron remains unspliced, the resulting E6E7 mRNA expresses oncogenic E

247 Consistent with this finding, the ratios of unspliced to spliced HIV-1 mRNAs in mouse cells expressi

248 osB pre-mRNA is regulated by the quantity of unspliced transcript available to the splicing machinery

249 Of these, 13 were spliced; a single unspliced transcript putatively encoded NS1.

250 terminal form is translated from the primary unspliced transcript to a stop codon within the intron u

251 eviously thought on the degradation of yeast-unspliced transcripts and plays an important role in dis

252 a protein was never detected indicating that unspliced transcripts are likely to be non-coding.

253 at PRGs generates low levels of full-length unspliced transcripts but fails to make mature, protein-

254 siRNAs interfere with the processing of the unspliced transcripts for the gp41 gene, tat, rev, and n

255 human PRP2/DHX16 results in accumulation of unspliced transcripts, similar to the outcome in yeast p

256 ch is transcript poor, containing only three unspliced transcripts.

257 m translation initiation codons contained in unspliced transcripts.

258 and five nucleoporins cause accumulation of unspliced tRNA, a hallmark of defective tRNA nuclear exp

259 membrane proteins also cause accumulation of unspliced tRNA, likely due to defective splicing on mito

260 2 leads to rapid accumulation of end-matured unspliced tRNAs in the nucleus.

261 that could be produced by translation of an unspliced UL29/28 transcript.

262 The ratio of spliced to unspliced UL37 transcripts also changed.

263 LTR) circles, and multiply spliced (ms-) and unspliced (us-) HIV-1 RNA concentrations were measured a

264 ytoma biopsy samples, FAK is expressed as an unspliced variant and migrates with a faster mobility si

265 Both spliced and unspliced variants of the new imprinted sense transcript

266 Retroviruses utilize an unspliced version of their primary transcription product

267 e resultant accumulation of both spliced and unspliced versions of some mRNAs in the cytoplasm.

268 y herpesviruses, SM transports predominantly unspliced viral mRNA cargo from the nucleus to the cytos

269 required for export of partially spliced and unspliced viral mRNA from nuclei of infected cells, and

270 ced viral mRNA and decreased accumulation of unspliced viral mRNA, resulting in decreased cell-associ

271 ction of Rej is to facilitate translation of unspliced viral mRNA.

272 ailure to export RRE-containing CAT mRNA and unspliced viral mRNAs to the cytoplasm, confirming that

273 ement (RRE) RNA to mediate nuclear export of unspliced viral mRNAs.

274 n 293T cells Rej modestly enhanced export of unspliced viral RNA (2.8-fold).

275 Rem is required for nuclear export of unspliced viral RNA and efficient expression of viral pr

276 , and indicates that cells containing solely unspliced viral RNA are a good marker for viral latency.

277 uclear export or accumulation of cytoplasmic unspliced viral RNA in 293T cells but not in most other

278 HIV-1 Gag selects and packages a dimeric, unspliced viral RNA in the context of a large excess of

279 ants also showed deficits in accumulation of unspliced viral RNA in the cytoplasm.

280 virus type 1 (HIV-1) proviruses that express unspliced viral RNA in vivo or about the levels of HIV R

281 This results in a decrease of unspliced viral RNA levels and an approximately 10-fold

282 For some retroviruses, transport of unspliced viral RNA to the cytoplasm is mediated by smal

283 veral orders of magnitude less abundant than unspliced viral RNA, was slightly enriched relative to a

284 not have consistent effects on the amount of unspliced viral RNA, whereas the amount of cell-associat

285 essary for the synthesis of Gag protein from unspliced viral RNA.

286 nsible for synthesis of Gag polyprotein from unspliced viral RNA.

287 ation codon that prevents degradation of the unspliced viral RNA.

288 orted from the nucleus, MLV actively exports unspliced viral RNAs to the cytoplasm.

289 ed in the nuclear export of both spliced and unspliced viral RNAs, and, finally, (iii) depletion of N

290 ve special relevance during the formation of unspliced viral transcripts (p < 0.0005).

291 Rev response element in stem IIB located on unspliced viral transcripts and subsequently oligomerize

292 ted enhancement of the cytoplasmic levels of unspliced viral transcripts.

293 in mRNA was significantly increased in NASH, unspliced X-box protein-1 (XBP-1) protein did not increa

294 e the characteristic of rapid elimination of unspliced Xbp-1 (Xbp-1u) and unspliced Hac1p, respective

295 Transcription of unspliced XBP-1 mRNA is up-regulated by IL-2 signals, wh

296 teraction through C-terminal domain with the unspliced XBP1 and the inositol requiring enzyme 1 alpha

297 damental signaling activities of spliced and unspliced XBP1 in breast cancer, establish NF-kappaB to

298 eporter constructs, we show that cytoplasmic unspliced XBP1 mRNA is efficiently spliced by activated

299 XBP1 splicing, occurring after depletion of unspliced XBP1.

300 Using tiling arrays, we show that many unspliced yeast pre-mRNAs accumulate in strains mutated