ライフサイエンスコーパス: urease

1 protected the activity of the electrosprayed urease.

2 llow the rapid access of urea to cytoplasmic urease.

3 mouse models to examine the contribution of urease.

4 more susceptible to fungi in the absence of urease.

5 mbly of the nickel-containing active site of urease.

6 ature coupled with pH on the activity of the urease.

7 d in the maturation of the nickel-containing urease.

8 orosarcina pasteurii and Helicobacter pylori ureases.

9 Urease, a major virulence factor for Cryptococcus neofor

10 Important components include a cytoplasmic urease; a pH-gated urea channel, UreI; and periplasmic a

11 acterization of Glycine max UreG (GmUreG), a urease accessory protein.

12 In plants, its activation requires three urease accessory proteins (UAPs), UreD, UreF, and UreG.

13 Urease accounts for up to 10% of the total cellular H. p

14 Its deletion abolishes urease activation and assembly, impairs cytoplasmic and

15 Formation of this complex and urease activation depend on expression of the cytoplasmi

16 structed for studying the in vivo effects on urease activation in recombinant Escherichia coli cells,

17 l for nickel delivery because UreG-dependent urease activation in vitro was observed only with UreG o

18 mes available in the leaves, indicating that urease activation is limited by nickel accessibility in

19 f UreG for nickel delivery during eukaryotic urease activation, inciting further investigations of th

20 Nickel is inserted into the urease active site in a GTP-dependent process with the a

21 e, closely resembling the traditional nickel-urease active site.

22 l reservoirs significantly impact the active urease activities achieved.

23 that promoted a long-term reduction in fecal urease activity and ammonia production.

24 nism, was sufficient to enhance P. mirabilis urease activity and increase disease severity, and enhan

25 This process requires functional urease activity and is independent of the cag pathogenic

26 environments, very little is known about the urease activity and regulation in specific bacteria othe

27 Using an in vitro urease activity assay, it is shown that the recombinant

28 binant protein is sufficient for recovery of urease activity in cell lysate from a HypA deletion muta

29 ur expectations, ammonia availability due to urease activity in P. mirabilis did not drive this gene

30 mirabilis and P. stuartii increased overall urease activity in vitro and disease severity in a model

31 We demonstrate that the lack of urease activity in vitro is not solely due to the amber

32 ther uropathogens also enhanced P. mirabilis urease activity in vitro, including recent clinical isol

33 e growth medium from 7.3 to 5, the wild-type urease activity increased threefold, but the activity in

34 The total urease activity level was decreased in the DeltaarsS str

35 de that the underlying mechanism of enhanced urease activity may represent a widespread target for li

36 rotein were analyzed by Western blotting and urease activity measurements.

37 el chelator did not significantly affect the urease activity of the wild type but decreased the activ

38 Urease activity potentially assists with survival fitnes

39 and P2 accounted for most of the changes in urease activity seen under various nitrogen conditions.

40 TI), delineated the contribution of enhanced urease activity to coinfection pathogenesis, and screene

41 Furthermore, total urease activity was increased during co-culture.

42 The urease activity was induced by urea, since complete and

43 and increase disease severity, and enhanced urease activity was the predominant factor driving tissu

44 tion pathogenesis, and screened for enhanced urease activity with other common CAUTI pathogens.

45 y, (1) exhaled antigen 85, (2) mycobacterial urease activity, and (3) detection by trained rats of di

46 In contrast to urease activity, IR treatment had a more pronounced effe

47 hundred two yeast isolates were screened for urease activity, melanin production, and glycine assimil

48 Urease activity, urea flux through the pH-gated urea cha

49 growth dynamics and impact of co-culture on urease activity.

50 russels sprouts) plants juices, on jack bean urease activity.

51 ast in part through synergistic induction of urease activity.

52 ontain a mutated ureD allele that eliminated urease activity.

53 creases the 2.7-kb transcript, and decreases urease activity.

54 ding motif result in a decrease in recovered urease activity.

55 resistance were also dependent on functional urease activity.

56 monia with ninhydrin, was developed to study urease activity.

57 e engineered murine gut microbiota to reduce urease activity.

58 lori requires two nickel-containing enzymes, urease and [NiFe]-hydrogenase, for efficient colonizatio

59 e extracytoplasmic function-sigma factors, a urease and a bile salt hydrolase.

60 in this study contribute to acid resistance (urease and amidase), acetone metabolism (acetone carboxy

61 N. moscoviensis possesses genes coding for a urease and cleaves urea to ammonia and CO2.

62 The EIS-urease and EIS-(PAMAM/CNT)-urease sensors showed similar

63 In bacteria, the UAPs interact with urease and facilitate activation, which involves the cha

64 creating the biosensor systems, the enzymes urease and glucose oxidase (GOD) were used as a bioselec

65 In the four cases studied (catalase, lipase, urease and glucose oxidase), the flow is driven by a gra

66 degradation products, into glutamate, using urease and glutamate dehydrogenase.

67 d in virulence phenotypes including laccase, urease and growth under oxidative/nitrosative stress.

68 We examined the effects of urease and hydrogenase assembly gene deletions on NikR a

69 irulence plasmid that carries genes encoding urease and lambda-toxin.

70 isolates, their iap/ibp plasmid also encoded urease and lambda-toxin.

71 require two virulence factors in particular: urease and MR/P fimbriae.

72 equires the nickel-containing metalloenzymes urease and NiFe-hydrogenase to survive this low pH envir

73 nhibitors UI, and double inhibitors DI, i.e. urease and nitrification inhibitors combined) regarding

74 Both the urease and the T3SS were previously shown to be essentia

75 treatment caused a substantial reduction in urease and trypsin inhibitor activities and considerable

76 is, the enzymatic activities of arginase and urease, and fungal gene expression in the extraradical a

77 They produced catalase, oxidase, urease, and H(2)S (lead acetate paper) but did not produ

78 i2+ ions into the nascent active site of the urease apoprotein (UreABC).

79 The results obtained with jack bean urease as a model urease, may contribute to the understa

80 Collectively, these data define cryptococcal urease as a pulmonary virulence factor that promotes imm

81 mber of virulence factors including laccase, urease as well as soluble polysaccharide and demonstrate

82 nization and denaturation of the immobilized urease, as such the model includes the effect of the fix

83 Additionally, SlyD functioned in urease assembly in strain 26695.

84 The loss of any component of urease assembly increased NikR activity under Ni2+-limit

85 ing that Zn(2+) may play a role on the plant urease assembly process, as suggested for bacteria.

86 ly onto a bare EIS [EIS-urease] sensor; (ii) urease atop the LbL film over the EIS [EIS-(PAMAM/CNT)-u

87 pylori phosphoglucosamine mutase (glmM) and urease B (ureB) gene of H. heilmannii and H. felis.

88 is induced by LPS from H. pylori, while the urease B subunit (UreB) is required for NLRP3 inflammaso

89 this study are comparable to the commercial urease based detection methods.

90 tiotemporal variation in pumping behavior in urease-based pumps and uncover the mechanisms behind the

91 onstrated, surpassing the conventional NWFET urease-based readout.

92 HP eradication was confirmed using urease breath test and stool antigen test.

93 Primers of urease C gene of H. pylori and Sau-3 and Hha I restricti

94 nd not absolutely required for colonization, urease can contribute to the pathogenicity of STEC.

95 iencies, such as chymotrypsin, fumarase, and urease, can be accurately and precisely estimated from a

96 Using previously published data from the urease-catalyzed hydrolysis of formamide, the commitment

97 The urease-catalyzed hydrolysis of hydroxyurea is known to e

98 The urease-catalyzed hydrolysis of urea displays feedback th

99 rease in pH after an induction period in the urease-catalyzed hydrolysis of urea was used to trigger

100 strain, a point mutation in ureD, encoding a urease chaperone protein, was identified, resulting in a

101 extracts and able to complement a DeltaureD-urease cluster in this host microorganism.

102 This analysis revealed that the urease cluster was the predominant factor in mediating r

103 On the other hand, the EIS-(PAMAM/CNT)-urease-CNT sensor exhibited a superior output signal per

104 film and another CNT layer [EIS-(PAMAM/CNT)-urease-CNT].

105 ROF electrodes were reversibly modified with urease-coated magnetic microparticles (MP) using a magne

106 complex was observed in cells producing all urease components.

107 me increase in the urea concentration in the urease-containing reaction medium.

108 In the intestine, bacterial urease converts host-derived urea to ammonia and carbon

109 ther urease-producing (wt and ure1::URE1) or urease-deficient (ure1) strains (H99) of C. neoformans w

110 fection (as compared with mice infected with urease-deficient organisms).

111 central nervous system in a process that is urease dependent, requires viability, and involves cellu

112 These complications were urease-dependent as they were not observed during coinfe

113 on promotes urolithiasis and bacteremia in a urease-dependent manner, at least in part through synerg

114 . pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher

115 gher in the protein extracts from transgenic urease-deprived plants than in extracts from non-transge

116 Urease diffusion measured using fluorescence correlation

117 as the sole source of nitrogen, thanks to a urease encoded by the ureDABCEFG operon.

118 The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling m

119 d on Ure protein sequences from a variety of urease-encoding bacteria demonstrates that the proline a

120 However, urease enzymatic activity is not related to its toxicity

121 s reveal that the L38P substitution enhances urease enzyme activity; however, the L205P substitution

122 er anoxic conditions is a consequence of the urease enzyme already present in the cells of the aerobi

123 In this study, we identify the Yersinia urease enzyme as the responsible oral toxin.

124 ut repeated injection of S. pasteurii as the urease enzyme degrades and/or becomes inhibited.

125 The STEC urease enzyme exhibited maximum activity near neutral pH

126 We show that diffusion of single urease enzyme molecules increases in the presence of ure

127 ion gradient, we show that both catalase and urease enzyme molecules spread toward areas of higher su

128 mposition of urine and the prevalence of the urease enzyme that hydrolyzes urea, minerals readily pre

129 al. (2015) report that H. pylori employs its urease enzyme to destroy urea to bring the concentration

130 regulation of the T3SS and activation of the urease enzyme, either of which would prevent E. ictaluri

131 The urease enzyme, encoded in the STEC chromosome, has been

132 me produces the urea used by the E. ictaluri urease enzyme.

133 had no effect on UreG's ability to activate urease, enzyme activity was essentially abolished in the

134 l that, although the benefit STEC gains from urease expression is modest and not absolutely required

135 sRNA103, and sRNA350--were shown to regulate urease, fimbria, and the LEE, respectively.

136 han 20% of its maximum, the amount of active urease formed per transcript of ure decreased almost lin

137 nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepa

138 We hypothesize that the gain and loss of the urease function is related to transmission strategies an

139 For future work, overexpression of urease fungitoxic peptides could be attempted as an alte

140 ve manner, controls the transcription of the urease gene cluster and alpha-carbonic anhydrase.

141 separate operons (ureAB and ureIEFGH) of the urease gene cluster.

142 ates transcription of the seven genes of the urease gene cluster.

143 efined consortium of 8 bacteria with minimal urease gene content.

144 ArsS, in addition to its role in urease gene transcription, is also involved in the recru

145 The urease genes are also present in Brucella, which has a f

146 The presence of highly similar urease genes in Nitrospira lenta from activated sludge,

147 are based on distributions of thaumarchaeote urease genes rather than activity measurements.

148 Arctic deep waters had a higher abundance of urease genes than those near the surface suggesting gene

149 ze ammonia, and a large fraction of them had urease genes, enabling the use of urea to fuel nitrifica

150 gation of the hydrolysis of semicarbazide by urease gives a relatively flat log V/ K versus pH plot b

151 with enzymes and/or other biomolecules, e.g. urease, glucose oxidase, hemoglobin, myoglobin (Mb), con

152 k, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate

153 Molecular dynamics simulations of urease have revealed a previously unobserved wide-open f

154 The NH(3) produced as urease hydrolyzes urea can aid in buffering bacteria in

155 ture, involving the LbL film with the enzyme urease: (i) urease immobilized directly onto a bare EIS

156 ing the LbL film with the enzyme urease: (i) urease immobilized directly onto a bare EIS [EIS-urease]

157 reD and then into the nascent active site of urease in a GTP-dependent process.

158 rrets, synthesizes a distinct iron-dependent urease in addition to its archetypical nickel-containing

159 ar behavior was also observed for the enzyme urease in aqueous solution.

160 the activity of the Ni(II)-dependent enzyme urease in bacterial cultures.

161 The increased level of urease in gastric acidity is due, in part, to acid activ

162 The increased level of urease in gastric acidity is due, in part, to acid activ

163 Here, we explore the role of urease in STEC pathogenicity.

164 soils and freshwater habitats, and of other ureases in marine nitrite oxidizers, suggests a wide dis

165 7-fold increase in the specific activity of urease, in contrast to the 70-fold increase seen in that

166 Urease inactivation in unsoaked samples was achieved at

167 Healthy urine is around pH 6, bacterial urease increases urine pH leading to the precipitation o

168 Therefore, urease inhibition by manuka honey is mainly due to MGO a

169 Urease inhibition of manuka honey correlates with its MG

170 lack MGO and DHA, showed significantly less urease inhibition.

171 concentration, as well as the efficiency of urease inhibition.

172 on from manuka honey with glyoxalase reduced urease inhibition.

173 The use of the urease inhibitor N-(n-butyl) thiophosphoric triamide (NB

174 NCP to test the ability of a newly developed urease inhibitor product Limus((R)) to decrease NH3 vola

175 of the examined organoselenium compounds as urease inhibitors against pathogenic bacteria.

176 Urease inhibitors are considered promising compounds for

177 ertilizers PCF, nitrification inhibitors NI, urease inhibitors UI, and double inhibitors DI, i.e. ure

178 n manuka honey, were identified as jack bean urease inhibitors with IC50 values of 2.8 and 5.0mM, res

179 represent a class of competitive reversible urease inhibitors.

180 nose-resistant Proteus-like (MR/P) fimbriae, urease, iron uptake systems, amino acid and peptide tran

181 Urease is a ubiquitous nickel metalloenzyme.

182 Urease is an essential component of gastric acid acclima

183 ic yeast Hansenula polymorpha and commercial urease is described.

184 Expression of urease is essential for gastric colonization by Helicoba

185 Although the iron-urease is less active than the nickel-enzyme, its activi

186 The iron-urease is oxygen-labile, with the inactive protein exhib

187 s and product egress from the active site of urease is tightly controlled by an active-site flap.

188 Failure of an E. ictaluri urease knockout mutant to increase the ECV pH in the in

189 des, physisorbed analytes were targeted with urease-labeled antibodies, and the urease on the pathoge

190 The urease levels and activity of the DeltaarsS strain after

191 anism and roles of each accessory protein in urease maturation are the subject of ongoing studies, wi

192 ry to [NiFe]-hydrogenase and participates in urease maturation.

193 ts obtained with jack bean urease as a model urease, may contribute to the understanding of bacterial

194 tions catalyzed by glucose oxidase (GOx) and urease, measurements of proteins by BCA assay, analysis

195 probe/sensor with the ability to detect the urease-mediated hydrolysis of urea in aqueous solution.

196 with the determination of urea in plasma by urease-mediated hydrolysis of urea.

197 fungal Mn biomineralization process based on urease-mediated Mn carbonate bioprecipitation [24].

198 This urease-mediated synthesis yields nearly monodisperse TiO

199 kalinized urinary tract, a characteristic of urease-mediated urea hydrolysis during P. mirabilis infe

200 ated by FlgS, underscoring the importance of urease membrane recruitment and activation in periplasmi

201 Urease membrane recruitment following prolonged acid exp

202 Assembly of the Klebsiella aerogenes urease metallocenter requires four accessory proteins, U

203 In the presence of urea, the urease-modified rGO FETs showed a shift in the Dirac poi

204 Mice were infected with P. mirabilis or a urease mutant, P. stuartii, or a combination of these or

205 erved during coinfection with a P. mirabilis urease mutant.

206 ost and bacterial protein synthesis, but not urease, NapA, VacA, CagA, or CagT.

207 majority of EHEC strains are phenotypically urease negative under tested conditions.

208 A urease-negative, fusiform, novel bacterium named Helicob

209 gical roles have been demonstrated for plant ureases, notably in toxicity to other organisms.

210 The TEM images of urease NPs showed their size in the range, 18-100nm with

211 eted with urease-labeled antibodies, and the urease on the pathogens hydrolyzed urea to ionic species

212 yer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect tr

213 Conversely, deletion of the urease operon in Y. pseudotuberculosis rendered it nonto

214 ble solute glycine betaine, ure genes of the urease operon, and mscL encoding a mechanosensitive chan

215 r cells, highly upregulated loci include the urease operon, the pyrimidine biosynthesis operon, the b

216 thaumarchaeal-related scaffold with the full urease operon.

217 7:H7 isolates and correlates with a negative urease phenotype in vitro.

218 10-fold more Ni associated with their total urease pools, even though all of the strains expressed s

219 tion, particularly by P. mirabilis and other urease-positive bacteria.

220 These findings suggest that urease-positive fungi could play an important role in th

221 lly induced mineralization, and suggest that urease-positive fungi may play a potential role in the s

222 The urease-positive fungi Pestalotiopsis sp. and Myrothecium

223 In this research, the urease-positive fungus Neurospora crassa was investigate

224 settings, and polymicrobial colonization by urease-positive organisms, such as Proteus mirabilis and

225 of the O157:H7 strains, neither the in vitro urease-positive phenotype nor the ureD sequence is phylo

226 The urease-positive species Proteus mirabilis and Providenci

227 rformed with three phylogenetically distinct urease-positive strains demonstrated that the STEC ure g

228 eitol, a total reactivation of the inhibited urease proceeded for the tested plants except for the on

229 The enzyme urease produces ammonia and enables bacteria to adapt to

230 Urease produces NH(3) and CO(2), neutralizing entering p

231 del of murine infection that utilizes either urease-producing (wt and ure1::URE1) or urease-deficient

232 Infection with urease-producing C. neoformans was associated with a hig

233 was markedly increased in mice infected with urease-producing C. neoformans.

234 Results indicate that mice infected with urease-producing strains of C. neoformans demonstrate a

235 Proteus mirabilis is a model organism for urease-producing uropathogens.

236 The presence of P. stuartii, regardless of urease production by this organism, was sufficient to en

237 tored through omeprazole, demonstrating that urease production in L. reuteri is mainly devoted to ove

238 be identified, but genes for a multi-subunit urease protein complex are present in the genome.

239 he strains expressed similar levels of total urease protein.

240 exposure increased the level and activity of urease proteins at the membrane in the wild type but not

241 tion, is also involved in the recruitment of urease proteins to the inner membrane to augment acid ac

242 medically important dinuclear nickel enzyme urease provides an excellent model for studying metalloc

243 We also show that urease reaches its full activity several days after nick

244 assay suggests that ammonia produced by the urease reaction mediates the pH increase.

245 luding a Michaelis-Menten expression for the urease reaction with a bell-shaped rate-pH dependence.

246 The onion juice modified urease, regained only half of the initial activity.

247 Because urease-related mortality eliminates 30-40% of infective

248 low-molecular-weight inhibitors of bacterial ureases reported to date.

249 mM for creatinine deaminase and 0.139 mM for urease, respectively.

250 S [EIS-(PAMAM/CNT)-urease] sensor; and (iii) urease sandwiched between the LbL film and another CNT l

251 se immobilized directly onto a bare EIS [EIS-urease] sensor; (ii) urease atop the LbL film over the E

252 p the LbL film over the EIS [EIS-(PAMAM/CNT)-urease] sensor; and (iii) urease sandwiched between the

253 The EIS-urease and EIS-(PAMAM/CNT)-urease sensors showed similar sensitivity (~18 mV/decade

254 the nickel-based metallocenter of the enzyme urease; several of the processing, transport, and medica

255 Attraction to urea requires that H. pylori urease simultaneously destroys the signal.

256 aqueous solution by taking advantage of the urease specific inhibition.

257 Within 30 min at pH 4.5, the urease structural subunits, UreA and UreB, and the Ni(2+

258 he ionization and denaturation states of the urease subject to the environmental conditions.

259 e the for metabolic processes important urea-urease system, with sensors based on spray-coated CNT-FE

260 ve endoscopy, histological assessment, rapid urease test and breath test were performed before and ei

261 ection and the HP virulence factor CagA by a urease test and PCR.

262 d biopsy histological examination plus rapid urease test indicating H. pylori infection; 2) gastric I

263 Unchanged substrate in a negative rapid urease test may be reused to detect Helicobacter pylori

264 The Rapid Urease Test was applied to fragments of the antral mucos

265 ., culture, histology Giemsa stain and rapid urease test) and non-invasive tests (anti-H. pylori IgG)

266 , 97%, 91%, and 66%, respectively, for rapid urease test).

267 n esophagogastroduodenoscopy (EGD) for rapid urease test, histology and PCR examination for Helicobac

268 H. pylori status was determined by the urease test, histology and presence of H. pylori ureA.

269 followed by histology, culture, and a rapid urease test, in order to obtain a consensus patient infe

270 r pylori requires nickel for hydrogenase and urease that are essential for acid viability), the "traf

271 rdsiella ictaluri produces an acid-activated urease that can modulate environmental pH through the pr

272 These bacteria secrete the enzyme urease that converts urea into carbon dioxide (CO2) and

273 ed component of two enzymes, hydrogenase and urease, that have been shown to be important for in vivo

274 Urease, the enzyme responsible for urea hydrolysis, and

275 ion was characterized to assess the roles of urease, the VirB type IV secretion system, and lipopolys

276 for up to 180 min activates total bacterial urease threefold.

277 Most notoriously, this pathogen uses urease to catalyze the formation of kidney and bladder s

278 elicobacter pylori possesses a highly active urease to support acid tolerance.

279 Here we used a hydrolytic enzyme, urease, to modify the solution environment around a wate

280 lyaniline), or biocatalytic (glucose oxidase/urease) triggers.

281 Effects of IR treatment on urease, trypsin inhibitor, lipoxygenase-1 and lipoxygena

282 cussed, suggesting that de novo synthesis of urease under anoxic conditions is not possible and that

283 Ur/Nr-NiO/ITO/glass) after immobilization of urease (Ur) shows excellent biosensing response characte

284 ) indicates the high affinity of immobilized urease (Ur) towards the analyte (urea).

285 n efficient matrix for the immobilisation of urease (Ur), the specific enzyme for urea detection.

286 y expressed in planta, we demonstrate that a urease-UreD-UreF-UreG complex exists in plants and show

287 Urease (Urs) and glutamate dehydrogenase (GLDH) are cova

288 ction is achieved by immobilizing the enzyme urease via certain surface functionalization techniques

289 s increase was significantly attenuated when urease was inhibited with pyrocatechol, demonstrating th

290 Despite the low yield of the ROS, jack bean urease was shown to be inactivated when the enzyme solut

291 uorescence lifetime of SNARF-1 conjugated to urease were not sufficient to explain the increase in di

292 n, columns with defined zones of immobilized urease were used to examine the distribution of calcium

293 he regulation of the nickel-dependent enzyme urease which is critical for the organism's survival in

294 i survives in acidic conditions by producing urease, which catalyzes hydrolysis of urea to yield ammo

295 ce factors required for cluster development: urease, which is required for urolithiasis, and mannose-

296 the urea sensors are biosensors and utilize urease, which limit their use in harsh environments.

297 c reactions catalyzed by glucose oxidase and urease, which results in a restoration of the photolumin

298 oviensis to supply ammonia oxidizers lacking urease with ammonia from urea, which is fully nitrified

299 ssembly of a pH-regulatory complex of active urease with UreI provides an advantage for periplasmic b

300 The effect of urease within the lung, the primary site of most invasiv