ライフサイエンスコーパス: uridylylation

1 and RNAs without affecting CRE-dependent VPg uridylylation.

2 synthesis did not inhibit CRE-dependent VPg uridylylation.

3 refuting the use of 3AB as the donor for VPg uridylylation.

4 recursors 3BC and/or 3BCD being employed for uridylylation.

5 of the compounds significantly inhibited VPg uridylylation.

6 ons within the CRE RNA structure prevent VPg uridylylation.

7 ue on the back of the thumb required for VPg uridylylation.

8 RNA that can be considered critical for VPg uridylylation.

9 NA replication is to act as template for VPg uridylylation.

10 NA-primed reactions but exhibits greater VPg uridylylation activity due to more efficient recruitment

11 of the cre RNA to serve as template for VPg uridylylation and abolished RNA infectivity.

12 f pre-mRNA polyadenylation, guide RNA (gRNA) uridylylation and annealing to mRNA, and editing reactio

13 Instead, the main defect is very slow uridylylation and deuridylation.

14 The wild-type enzyme undergoes uridylylation and deuridylyation about 10(6) times faste

15 alled the T-loop, which contains the site of uridylylation and is believed to be very important for c

16 and replication of viral RNA showed that VPg uridylylation and negative-strand RNA synthesis occurred

17 role of the tyrosine hydroxyl of VPg in VPg uridylylation and negative-strand RNA synthesis.

18 disrupt the structure of the CRE prevent VPg uridylylation and positive-strand RNA synthesis.

19 al sequences [cre(2C)] as a template for VPg uridylylation and requires the addition of proteinase 3C

20 AAACA) was less severe but reduced both VPg uridylylation and virus yield.

21 Both uridylylation and zinc binding influence the stability a

22 alyzes guide RNA, ribosomal RNA, and mRNA 3'-uridylylation, and RNA editing TUTase 2 acts as a subuni

23 These mutants also decreased 3B uridylylation, arguing that the binding sites for the me

24 iruses and likely involved in templating VPg uridylylation as in other picornaviruses, despite its si

25 a template for reiterative CRE-dependent VPg uridylylation before and during negative-strand RNA synt

26 rmation about the true in vivo substrate for uridylylation by 3D(pol).

27 usion, the efficiency and specificity of VPg uridylylation by picornavirus polymerases is greatly inf

28 e identify several additional substrates for uridylylation by poliovirus 3D polymerase.

29 tation in HRV16 VPg moderately increased its uridylylation by PV 3D(pol) in vitro, suggesting that it

30 shown to be the primary RNA template for VPg uridylylation by the PV RNA polymerase 3D(pol), we have

31 eceptors, ATase, and NRII (NtrB), and on PII uridylylation by the signal transducing UTase/UR.

32 degreesC, the first-order rate constant for uridylylation by UDP-glucose is 281 +/- 18 s-1, and for

33 e chirality experiments also showed that VPg uridylylation can occur by a single step; therefore, the

34 ferase (ATase), and is subject to reversible uridylylation, catalyzed by the uridylyltransferase/urid

35 nto the architecture of the picornavirus VPg uridylylation complex.

36 ient recruitment to and retention in the VPg uridylylation complex.

37 or 3Dpol recruitment to and retention in the uridylylation complex.

38 that leads to production of more processive uridylylation complexes.

39 sitivity and concentration robustness in the uridylylation cycle of the PII protein, and in the phosp

40 of GlnK to block the channel is regulated by uridylylation/deuridylylation at Y51.

41 Finally, determinants of VPg uridylylation efficiency suggest formation and/or collap

42 functionally for the poliovirus cre in both uridylylation in vitro and RNA replication in vivo.

43 ontains the minimal cre RNA required for VPg uridylylation in vitro, (ii) the location of the cre RNA

44 Mutations in VPg, which interfered with uridylylation in vitro, were found to abolish viral grow

45 rus 14 under solution conditions optimal for uridylylation in vitro.

46 t is not yet known whether the substrate for uridylylation in vivo is the free peptide itself or one

47 RNA uridylylation is critical for the expression of the mito

48 ons, several lines of evidence indicate that uridylylation is not required for relief of NifL inhibit

49 r all polymerases, a front-loading model for uridylylation is proposed.

50 the predominant product of CRE-dependent VPg uridylylation, is a putative primer for the poliovirus R

51 merase active site specifically decreased 3B uridylylation, likely affecting steps subsequent to bind

52 imental data indicate that CRE-dependent VPg uridylylation lowers the K(m) of UTP required for viral

53 ipts, which are stabilized by RET1-catalyzed uridylylation, may direct a nucleolytic cleavage of mult

54 y, although covalent modification of GlnK by uridylylation normally occurs under N-limiting condition

55 (PIRCs) to determine when CRE-dependent VPg uridylylation occurs relative to the sequential synthesi

56 of the uridylylation reaction were observed: uridylylation of 3D polymerase and 3CD protein was stimu

57 te was poly(A) instead of the 15-nt RNA, the uridylylation of 3D polymerase itself became intramolecu

58 ompletely, and in the absence of GlnD, whose uridylylation of GlnB is also normally essential for Nif

59 The results suggest that the role of uridylylation of GlnB is primarily to shift the equilibr

60 croM, which is higher than that required for uridylylation of GlnJ in vitro, about 5 microM.

61 modified GlnK stimulates NifL inhibition and uridylylation of GlnK in response to nitrogen limitation

62 the primary template for HRV2 3D(pol) in the uridylylation of HRV2 VPg, yielding VPgpU and VPgpUpU.

63 to the lower 2-KG requirement for productive uridylylation of P(II) by GlnD.

64 The reversible uridylylation of PII indirectly controls the activity of

65 The value of Km for uridylylation of Q168R is slightly higher than for the w

66 3'-Uridylylation of RNA is emerging as a phylogenetically w

67 However, the activities responsible for 3' uridylylation of rRNAs and mRNAs, and the roles of these

68 segments appear to serve as template for the uridylylation of the genome-linked protein, VPg, providi

69 op structure that serves as the template for uridylylation of the peptide primer VPg by the viral RNA

70 that templates 3D(pol) polymerase-catalyzed uridylylation of the protein primer for RNA synthesis, V

71 HRV-14) and poliovirus because they template uridylylation of the protein primer, VPg, by the polymer

72 structure functions as the template for the uridylylation of the viral protein VPg.

73 This priming step requires uridylylation of the VPg peptide by the viral polymerase

74 This conclusion is supported by in vitro uridylylation of these proteins, the ability of a mutant

75 entially used as a template for the in vitro uridylylation of VPg catalyzed by 3D(pol) in a reaction

76 3C, 3CD, and 3BCD proteins that control the uridylylation of VPg during the initiation of viral repl

77 cornavirus RNAs serves as a template for the uridylylation of VPg, resulting in the synthesis of VPgp

78 the canonical UU donated by CRE(2C)-mediated uridylylation of VPg, we hypothesized that a functional

79 pper stem region of PV-cre(2C) also affected uridylylation of VPg.

80 act directly with the stem of the cre during uridylylation of VPg.

81 the altered 3Dpol to catalyze oriI-templated uridylylation of VPg.

82 sed as the primary template for the in vitro uridylylation of VPg.

83 iation of RNA replication, CRE-dependent VPg uridylylation provides a mechanism for a more robust ini

84 ate constant (3.7 x 10(-)(2) s(-)(1)) and de-uridylylation rate constant (0.5 x 10(-)(2) s(-)(1)) res

85 ty of S161A was directly related to impaired uridylylation rate constant (3.7 x 10(-)(2) s(-)(1)) and

86 minate, or severely reduce, the in vitro VPg-uridylylation reaction and produce replication phenotype

87 The uridylylation reaction either with transcripts of cre(2C

88 se interfaces have pronounced effects on the uridylylation reaction in vitro.

89 This study examined the effects on the VPg uridylylation reaction of the RNA template sequence, the

90 so examined the metal specificity of the VPg uridylylation reaction on a poly(A) template.

91 on the efficiency and the specificity of the uridylylation reaction were observed: uridylylation of 3

92 k side of the polymerase molecule during the uridylylation reaction, opposite to that predicted to bi

93 y to function as template in an in vitro VPg uridylylation reaction, suggesting that these functions

94 other function(s) in addition to the direct uridylylation reaction.

95 g the slide-back step and also modulates the uridylylation reaction.

96 nt VPgs were tested as substrates in the VPg uridylylation reaction.

97 y to serve as a template in the in vitro VPg uridylylation reaction.

98 d the viral specificity in 3D(pol)-catalyzed uridylylation reactions between poliovirus (PV) and huma

99 RNA uridylylation reactions catalyzed by terminal uridylyl t

100 Uridylylation reactions of Q168R with UDP-glucose procee

101 Moreover, in vitro uridylylation reactions showed that membrane-bound 3AB i

102 specificities and metal requirements of the uridylylation reactions.

103 ) are known: RET1 catalyzes guide RNA (gRNA) uridylylation, RET2 executes U insertion mRNA editing, a

104 NA elongation or a protein primer during the uridylylation stage of replication initiation.

105 to the NRII-NRI monocycle in the form of its uridylylation state and is also the receptor of the anta

106 Membrane binding is dependent on the uridylylation state of GlnK and is modulated according t

107 ations, we conclude that in intact cells the uridylylation state of PII is regulated mainly by the gl

108 of nitrogen assimilation by controlling the uridylylation state of the PII signal transduction prote

109 Equilibrium uridylylation studies showed that neither Cys 160 nor Se

110 By using an in vitro VPg uridylylation system for HRV-14 that recapitulates biolo

111 data permit elaboration of our model for VPg uridylylation to include the use of precursor proteins a

112 GalT, and all of the active sites underwent uridylylation to the UMP-enzyme, similar to wild-type Ga

113 f gRNAs and rRNAs, while transcript-specific uridylylation triggers 3' to 5' mRNA decay.

114 All three proteins were found to undergo uridylylation under ammonium starvation conditions, pres

115 The defect to uridylylation was a result of the inability of 3CD to st

116 ontaining the Y51N alteration at the site of uridylylation, was not uridylylated by the UTase/UR and

117 imal system to evaluate the mechanism of VPg uridylylation, we show that the active complex contains

118 when CRE-disrupting mutations prevented VPg uridylylation, whereas correspondingly low concentration

119 The site of PII uridylylation, Y51, is located at the apex of the solven

120 A-dependent RNA polymerase, 3D, for covalent uridylylation, yielding mono and di-uridylylated product