ライフサイエンスコーパス: variable domain

1 ace receptor selects OME partners based on a variable domain.

2 hain homodimers is mediated through a single variable domain.

3 somatic mutations distributed throughout the variable domain.

4 , important function for this evolutionarily variable domain.

5 able domain scaffold and a fixed light chain variable domain.

6 rotein, a dimeric immunoglobulin light chain variable domain.

7 b with the framework sequences of a human Ab variable domain.

8 l terminal ends, respectively, and a central variable domain.

9 ed by synthetic peptides derived from the LC variable domain.

10 iate via their amino-terminal immunoglobulin variable domain.

11 e amino and carboxyl termini and one central variable domain.

12 iable regions also may be present within the variable domain.

13 ffinity-labels a single site in the antibody variable domain.

14 falciparum expressed in the CDR3 of the same variable domain.

15 strate-binding domain attached to the 10-kDa variable domain.

16 each monomer comprising one constant and one variable domain.

17 sequences of antibody variable fragment (Fv) variable domains.

18 artilaginous fish and in camelid heavy-chain variable domains.

19 f the immunogenicity derived from the Fc and variable domains.

20 linkages at the base of the surface-exposed variable domains.

21 olves stabilization of heavy and light chain variable domains.

22 n might also depend on IgA carrying specific variable domains.

23 to the weak immunogenicity of immunoglobulin variable domains.

24 ceae have unique extensions to the V5 and V6 variable domains.

25 act light chains and recombinant light chain variable domains.

26 s, or parts of these structural units of the variable domains.

27 ds were found for different fragments of the variable domains.

28 25% of serum IgG contains glycans within the variable domains.

29 was found to be higher in CH1 and CL than in variable domains.

30 induced change in the orientation of the two variable domains.

31 ficial mutations can be found throughout the variable domains.

32 h the interface of the heavy and light chain variable domains.

33 delta-H chains, which incorporated Cmu1 and variable domains.

34 MAb MoPn-40 binds to a linear epitope in the variable domain 1 (VD1), and MAb MoPn-32 recognizes the

35 In many isolates variable domain 1 is flanked by direct repeat elements,

36 deduced amino acid sequences encoded within variable domain 1 predict them to be hydrophilic, surfac

37 nce changes in gp160, principally within the variable domain 1/variable domain 2, variable domain 3,

38 und to be clustered in two distinct domains (variable domains 1 and 2).

39 60, principally within the variable domain 1/variable domain 2, variable domain 3, and variable domai

40 TCR ligand, human T-cell receptor beta chain variable domain 2.1.

41 though numerous changes were observed in the variable domain 3 loop and in other regions of gp160.

42 hin the variable domain 1/variable domain 2, variable domain 3, and variable domain 4 loops.

43 MAbs to variable domain 4 (VD 4) of MOMP have been described tha

44 1/variable domain 2, variable domain 3, and variable domain 4 loops.

45 protein, TIGIT, containing an immunoglobulin variable domain, a transmembrane domain and an immunorec

46 One contains two variable domains: a somatically recombined V and a prejo

47 [aa] 144 to 187), and one mapped to the CT-1 variable domain (aa 386 to 480).

48 though the changes in the association of the variable domains affect the precise positioning of resid

49 between the two structures is 0.6 A for the variable domain and 0.7 A for the constant domain.

50 sseria gonorrhoeae include an immunodominant variable domain and one or more invariable domains that

51 d with random mutagenesis of its heavy-chain variable domain and panning against sGHeV.

52 Additional regions of the N-terminal variable domain and the constant domains, however, great

53 ion extended off the side of the light chain variable domain and was not required for neutralization.

54 ural and sequence similarities between mouse variable domains and a repertoire of human antibody germ

55 mational changes that expose epitopes in the variable domains and disrupt quaternary epitopes in the

56 the interface of the heavy- and light-chain variable domains and form the antigen-binding site, the

57 ntarity-determining region of immunoglobulin variable domains and has been shown to be conformational

58 e only gene with both central and C-terminal variable domains and has three to four times more intron

59 n the interfacial region of the constant and variable domains and highlight the general resistance of

60 en to be near the linker regions between the variable domains and the constant domains of these antib

61 evel of sequence diversity observed in human variable domains and the requirement to maintain antigen

62 ubclass, containing the same antigen-binding variable domains and with equal binding to Abeta, MABT s

63 the development of human-based single chain variable domain antibody fragments (scFvs) directed agai

64 A model BsAb was constructed using a single variable domain antibody to mouse platelet-derived growt

65 get antigens, by genetically fusing a single variable domain antibody to the N terminus of the light

66 Here, we report a human heavy-chain variable domain antibody, HN3, with high affinity (Kd =

67 A bispecific, single variable-domain antibody (anti-TNFRI moiety plus an albu

68 ysis of these V-NARs has revealed an unusual variable domain-antigen interaction.

69 Somatic mutations within the antibody variable domains are critical to the immense capacity of

70 that have only heavy chains; unpaired heavy variable domains are responsible for antigen binding.

71 te the lack of a canonical hinge region, the variable domains are spaced appropriately wide for bindi

72 When expressed recombinantly these variable domains are termed single domain antibodies (sd

73 entarity determining region, suggesting that variable domains are the products of antigen-driven B ce

74 c form of the Mcg immunoglobulin light chain variable domain as the quaternary unit required for amyl

75 se sequence was sampled by exchanging single variable domains as well as larger blocks of contiguous

76 ons of homology in 3H1 heavy and light chain variable domains, as well as in the framework regions.

77 When using variable domain backbones from the crystal structures, t

78 We identify structurally variable domains between cell types and examine the unde

79 only in the diverse epitopes to which their variable domains bind but also in the various effector m

80 All heavy chain-only antibody variable domains bind HypE when expressed as GFP fusions

81 h by developing high-affinity Fv from single variable domains binding to RAS and LMO2 oncogenic prote

82 single-chain antibody component in which the variable domain binds to hFcgammaRI [anti-hFcgammaRI (H2

83 We demonstrate that each of the three variable domains binds to the same variable domain in an

84 The variable domain bound to virions and blocked HSV infecti

85 sociated heavy (V(H)) and light (V(L)) chain variable domains, but in camels and llamas, the binding

86 iversification of CDRs of the immunoglobulin variable domain by mutagenic PCR.

87 ve orientation of the heavy- and light-chain variable domains can create another source of structural

88 B-cell MoAb (chCLL-1) constructed using the variable domains cloned from the murine Lym-2 (muLym-2)

89 affect ligand binding efficiency as well as variable domain conformation.

90 In contrast, large regions of the variable domain could be excised without reducing the in

91 that the increased malleability of mAb 9-40 variable domains could account for functional difference

92 CD) of 180-220 amino acids, and a C-terminus variable domain (CVD) of 30-60 amino acids.

93 soforms engage in homo-dimerization coupling variable domain D2 with D2, and D3 with D3.

94 volution of the previously defined EIAV gp90 variable domains demonstrated distinct differences in th

95 that Nanobodies, i.e. immunoglobulin single variable domains derived from naturally occurring heavy

96 tely 300 amino acids long) and an N-terminal variable domain differing in sequence and size (30-120 a

97 formation by stabilizing the Mcg light chain variable domain dimer and shifting the equilibrium away

98 e construction of an Ab fusion molecule with variable domains directed against the tumor-associated A

99 Variable domain displayed no binding, and KH2 or KH3 dom

100 The ability to cause variable domain dissociation was dependent on whether mo

101 highly diversified library of human antibody variable domains (domain antibodies) and used it for sel

102 ve orientation of the light- and heavy-chain variable domains during evolution of the CH103 lineage.

103 ive natural buried lysine embedded in a dual variable domain (DVD) format.

104 ted for a dimeric immunoglobulin light chain variable domain, employing pressure, temperature, and so

105 Immunoglobulin gene variable domains encoding the antibodies that react with

106 nti-interleukin (IL)-6 antibody generated by variable domain engineering, to achieve subpicomolar aff

107 Positioning of incorrect Leu/Ile residues in variable domains, especially in CDRs (complementarity de

108 alculated selection conditions with antibody variable-domain exchange to direct individual antibody v

109 Variable domains exhibit no symmetry relationship as a c

110 ly purified polypeptides, thereby permitting variable domain exon assembly by using this fully define

111 i-glycosyltransferases) and their N-terminal variable domain facing the cytosol.

112 rK ORFs shows the presence of seven discrete variable domains flanked by highly conserved regions.

113 of the Fab molecules and the sequence of the variable domain for each of the Fab molecules have been

114 aid in the selection of more suitable mouse variable domains for antibody engineering to render them

115 d limited cleavage sites are observed in the variable domains for each chain, where the S-S spans the

116 f K12v, suggesting synergism of KH2, KH3 and variable domains for the binding activity.

117 luenza-virus hemagglutinin (HA):single-chain variable-domain fragment complexes, by studying a comple

118 CR genes with the desired specificity, or Ab variable domain fragments fused with T cell-signaling mo

119 LEN is a kappaIV immunoglobulin light chain variable domain from a patient suffering from multiple m

120 GFR-iRGD consisting of an anti-EGFR VHH (the variable domain from the heavy chain of the antibody) fu

121 between the heavy (VH) and light (VL) chains variable domains from 15 to 5 amino acids.

122 stability of LC dimers and their associated variable domains from AL amyloidosis patients and non-pa

123 re sequentially replaced with repertoires of variable domains from non-immunised human donors followe

124 ombinant immunotoxin containing an anti-CD22 variable domain (Fv) fused to truncated pseudomonas exot

125 onstructs are designed by combining antibody variable domains, generated by phage display or derived

126 We have used this intracellular single variable domain (IDab) format, based on a previously cha

127 Using dual variable domain Ig (DVD-Ig) technology, we constructed a

128 cells could not respond to the IgM/DNA dual variable domain Ig molecule, despite a normal response t

129 of bifunctional autoantibodies, IgM/DNA dual variable domain Ig molecules, to activate B cells throug

130 entical predominant V(H) gene with unmutated variable domain (IGHV1-3) for both IgG and IgM anti-HBc

131 in lacking two conserved cysteines in highly variable domain III of the extracellular region.

132 oglobulin G (IgG)-like molecule--termed dual-variable-domain immunoglobulin (DVD-Ig)--that can be eng

133 anti-von Willebrand factor humanized single-variable-domain immunoglobulin (Nanobody), inhibits the

134 the three variable domains binds to the same variable domain in an opposing isoform and identify the

135 ective D gene incorporation into the TCRbeta variable domain in the absence of other nuclear factors

136 ne substrate specificity, whereas the larger variable domain in the N-terminus might play a role in f

137 l involvement of sites in both the V2 and V3 variable domains in antibody recognition.

138 ht into how "matching" at all three pairs of variable domains in Dscam mediates isoform-specific reco

139 ion of monoclonal immunoglobulin light chain variable domains in the form of insoluble amyloid fibril

140 Although much is known about the role of variable domains in the neutralization breadth and poten

141 s of extracellular deposition of light chain variable domains, including amyloid fibrils and amorphou

142 to facilitate incorporation of two different variable domains into a single molecule.

143 ion containing both NaCl and 6-AHA, only the variable domain is released from the particle surface.

144 myloid fibrils of immunoglobulin light-chain variable domains is proposed as a general model for the

145 mechanochemical core of Drp1, absent of the variable domain, is sufficient to mediate GTP hydrolysis

146 olypeptide sequences within their GTPase and variable domains, known as the A-insert and the B-insert

147 The variable domain LEN was obtained from a patient who had

148 ly two of the 36 sequence differences in the variable domains, Leu(H47)Trp and Arg(H100)Trp, converts

149 rlap amino acids 64 to 104 of the N-terminal variable domain-like immunoglobulin domain.

150 We also show the variable domain limits premature Drp1 assembly in soluti

151 ween WbwK and WbsJ revealed that the smaller variable domains located in the C-terminus determine sub

152 Palpha1 directly binds to the immunoglobulin variable domain loop of purified human CD47 and that suc

153 cal studies demonstrate that, in addition to variable domain "matching," intramolecular interactions

154 of active RalA is due in part to its unique variable domain near the C terminus.

155 A model of the variable domain of 16G3 was generated from the primary s

156 cooperativity in a complex formed between a variable domain of a T cell receptor and a bacterial sup

157 This variable domain of an H chain-only Ab (VHH or nanobody)

158 The variable domain of an immunoglobulin (IG) sequence is en

159 LEN is the variable domain of an immunoglobulin light chain origina

160 ide binding site (NBS), found within the Fab variable domain of antibodies, remains a not-so-widely k

161 Gene assembly of the variable domain of antigen receptors is initiated by DNA

162 l PKCs (C2-2), a peptide from the N-terminal variable domain of epsilonPKC (epsilonV1-2) and a peptid

163 amelids comprises a single domain, named the variable domain of heavy chain of HCAbs (VHH).

164 thout the intra-domain disulfide bond of the variable domain of heavy chain or the variable domain of

165 combining a chimpanzee IgG light chain and a variable domain of heavy chain with a human constant Fc

166 The resulting mRNA coding for the variable domain of heavy-chain antibodies (VHH) were iso

167 hich implicates the 10-kDa carboxyl-terminal variable domain of Hsc70, is incorrect.

168 Amyloid fibrils from insulin and the variable domain of Ig light chain demonstrate induced CD

169 nts, VH, D, and JH, that together encode the variable domain of Ig.

170 of the variable domain of heavy chain or the variable domain of light chain.

171 n vitro reconstitution of BiP binding to the variable domain of light chains (VL).

172 In transfection studies, the isolated variable domain of PKC-epsilon selectively blocked exoge

173 expressing a fragment derived from the first variable domain of PKCdelta.

174 s that express a fragment encoding the first variable domain of PKCepsilon (amino acids 2-144), which

175 he CDR3-like loop within the membrane-distal variable domain of Skint-1 (Skint-1 DV).

176 anscribed spacer (ITS) regions and the D1-D2 variable domain of the 28S ribosomal DNA gene (28S), the

177 We used high-throughput sequencing of the variable domain of the antibody heavy chain from 14 zebr

178 immunodominant epitopes present in the third variable domain of the envelope glycoprotein, transmembr

179 binding site is comprised exclusively of the variable domain of the heavy chain (VHH).

180 ineered single-chain antibodies in which the variable domain of the heavy chain is joined to the vari

181 Nanobodies (Nbs), also known as the variable domain of the heavy-chain-only antibody (VHH),

182 ementarity determining region (CDR) 3 of the variable domain of the light chain (VL) of this antibody

183 A recombinant variable domain of the light chain SMA was used to form

184 e domain of the heavy chain is joined to the variable domain of the light chain through a peptide lin

185 Antibodies directed to purified recombinant variable domain of Tpr K can opsonize T. pallidum, Nicho

186 ion of rabbits with the purified recombinant variable domain of Tpr K provides significant protection

187 ly showed that di-mannose recognition by the variable domains of 2G12 is independent of domain exchan

188 The variable domains of a high affinity anti-CEA antibody, T

189 issect how mutations at all positions of the variable domains of a high-affinity anti-VEGF antibody G

190 nd also by using intracellular expression of variable domains of a neutralizing antibody fused to gre

191 The variable domains of antibodies and T-Cell receptors (TCR

192 The cleavages are mainly localized in the variable domains of both heavy and light chains, the res

193 Unlike the monomeric variable domains of camelid heavy chain antibodies (V(H)

194 Variable domains of camelid heavy chain-only antibodies

195 nkage proximal glycosylation adjacent to the variable domains of gp120 and begin to explain how this

196 d cost-effective production, the recombinant variable domains of heavy-chain-only antibodies (VHHs) a

197 -bounded region spanning the V1 and V2 hyper-variable domains of HIV-1 gp120.

198 The variable domains of Ig and T-cell receptor genes in vert

199 High-throughput sequencing of the variable domains of immune receptors (antibodies and T c

200 hologic deposition of monoclonal light chain variable domains of immunoglobulins as insoluble fibrils

201 , we designed anti-ETEC antibodies by fusing variable domains of llama heavy chain-only antibodies (V

202 lecules on biosensors was investigated using variable domains of llama heavy-chain antibodies (VHHs)

203 m2G7) was partly humanized (h2G7) by merging variable domains of m2G7 with human antibody-Fc backbone

204 Toxin binding to variable domains of T cell receptor beta chains (Vbeta)

205 rom a noncanonical interface between the two variable domains of the antibody.

206 "elbow" regions, which link the constant and variable domains of the Fab, can introduce disorder and

207 ith a high level of sequence homology in the variable domains of the heavy and light chains.

208 ormally single chain Fv fragments comprising variable domains of the immunoglobulin heavy (VH) and li

209 Nucleophilic sites in the paired variable domains of the light and heavy chains (VL and V

210 imers and probes were designed to target two variable domains of the ompA gene, VD2 and VD4.

211 from a hierarchical interaction between the variable domains of the OpaA protein of MS11mk.

212 mmunodominant epitopes of OspC reside in the variable domains of the protein.

213 First, in codons encoding highly variable domains of the proteins, there was a greater ac

214 thin the major outer membrane protein (MOMP) variable domains: one family of Da, D*, and D- and one f

215 ng site frequently comprises the heavy chain variable domain only (referred to as V(HH)).

216 ity determining region loop conformation and variable domain orientation.

217 Variable domain pair associations VL:VH for the Fabs are

218 LT-1 is similar to other immunoglobulin-like variable domains, particularly those of triggering recep

219 es demonstrated that although its N-terminal variable domain plays an essential role in optimizing Ca

220 demonstrates that large portions of antibody variable domain positions are open to mutation, and that

221 known regarding how antibodies with a single variable domain recognize small ligands.

222 Dimers of Mcg variable domains remain stable and soluble, yet become p

223 The nanobodies were obtained from a variable-domain repertoire library isolated from llamas

224 matic mutations in the heavy and light chain variable domains, respectively, a long HCDR3, and a dele

225 l structure of the human CD84 immunoglobulin variable domain revealed an orthogonal homophilic dimer

226 fragments (scFvs) with a single heavy chain variable domain scaffold and a fixed light chain variabl

227 (anti-IC) single-chain fragment of antibody variable domain (scFv) and a monoclonal antibody capable

228 ation and characterization of a single-chain variable domain (scFv) antibody isolated against oligome

229 The IgG, diabody, single-chain variable domain (scFv), and novel miniantibody formats,

230 lasts and amplified expressed immunoglobulin variable domain sequences by single-cell PCR.

231 C-terminal constructs, which lack central variable domain sequences, can oligomerize and localize

232 mber antibody and T-cell receptor amino-acid variable domain sequences.

233 bility is the result of alternative usage of variable domain sequences.

234 mbining site involving heavy and light chain variable domains shaped by somatic hypermutation and aff

235 ally folded intermediates of the light chain variable domain SMA in the presence of guanidine hydroch

236 enic kappa4 human immunoglobulin light-chain variable domain, SMA, associated with AL amyloidosis, we

237 y of a recombinant amyloidogenic light chain variable domain, SMA, on various surfaces was monitored

238 s of a recombinant amyloidogenic light chain variable domain, SMA, to determine whether partially fol

239 n of a recombinant amyloidogenic light-chain variable domain, SMA, with lipid vesicles.

240 integral to motor regulation is attached to variable domains so that the cell can target regulators

241 he functional paratopes with the 3D antibody variable domain structure as input.

242 t, designated as gammaE, was isolated with a variable domain structure similar to a gammaB-subunit bu

243 All the variable domains studied readily form amyloid fibrils, w

244 , K261 and K327) are found in the C-terminal variable domain that is responsible for DNA recognition,

245 domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recogniti

246 Antigen-specific single immunoglobulin variable domains that bind to native antigens can be iso

247 ptors were prepared which contained antibody-variable domains that bound the capsid in place of the T

248 response by selecting mutations in antibody variable domains that enhance antigen binding.

249 ynamic coupling between the TCR constant and variable domains that is dampened upon ligation.

250 beta subunits displaying immunoglobulin-like variable domains that recognize peptide antigens associa

251 selection strategy to create human antibody variable domains that resisted heat aggregation.

252 this chimeric molecule contains a rearranged variable domain, the first constant domain of mu, and se

253 zing protection from HIV infection via their variable domains, the antibody constant domain provides

254 This synaptogenesis depended on TCR variable domains, the kinase Lck and the integrin alpha(

255 Central variable domains themselves are monomeric and have no ta

256 In addition, interspersed within the VlsE variable domain there are six invariable regions (IR1-6)

257 Antibodies engineered in their variable domain to express epitopes of heterologous anti

258 reverse turns, allows each pair of the three variable domains to "match" in an antiparallel fashion.

259 omain exchange to direct individual antibody variable domains to desired epitopes.

260 used with immunoblotting and sequencing IgG variable domains to screen, select, and characterize ant

261 e orientations of the heavy- and light-chain variable domains using side-chain rotamer sampling in th

262 emonstrated the ability to sequence antibody variable domains using the Ion Torrent PGM platform.

263 gainst an Env and found that the heavy chain variable domain (V(H)) of this antibody, designated as m

264 y germline mutations only in the heavy chain variable domain (V(H)) that ultimately led to an increas

265 g an in vitro-evolved autonomous heavy chain variable domain (V(H)H-RIG), we have investigated the li

266 The antibody light chain variable domain (V(L))(1) and myelin protein zero (MPZ)

267 utations of human immunoglobulin light chain variable domain (V(L)).

268 third hypervariable loop of the light chain variable domain (V(L))] adopts the type 1 canonical stru

269 quencing of individual B cell immunoglobulin variable domains (V genes).

270 bodies consists of the heavy and light chain variable domains (V(L) and V(H) domains).

271 onse away from V3 to an epitope in the first variable domain (V1) of gp120.

272 diversity among them was observed in the two variable domains (V1 and V2), semivariable domain (SV),

273 ty to antibodies directed to epitopes in the variable domains (V2 and V3) that are buried in the pare

274 ly directed toward determinants in the third variable domain (V3) of the major envelope glycoprotein,

275 s included those at the interface of the TCR variable domains (Valpha and Vbeta) and surface-exposed

276 ryl due to unpaired cysteine residues in the variable domains varied for different antibodies.

277 lecules and T-cell receptor (TCR) beta-chain variable domains (Vbetas).

278 says, we show that removal of the regulatory variable domain (VD) in Drp1 enhances formation of a fun

279 Antibodies to variable domain (VD)1 of the MOMP were detected in serum

280 bules, but the function of the corresponding variable domain (VD, or insert B) of Drp1 is unknown.

281 hase scanning of overlapping octapeptides of variable domains (VDs) of the major outer membrane prote

282 method to site-specifically label VHHs [the variable domain (VH) of a camelid heavy-chain only antib

283 ith the constant region (Fc) and heavy chain variable domain (VH) of IgG, respectively.

284 alysed the pairings of heavy and light chain variable domains (VH and VL) in 365 human IgG+ B cells f

285 oclonal antibody requires that complementary variable domains (VH or VL) bind to the same antigenic s

286 affected by the relative orientation of the variable domains, VH and VL.

287 y immunizing mice with a thermally denatured variable domain (VL) fragment of the human kappa4 Bence

288 positions in its former antibody light chain variable domain (VL) interface to reduce hydrophobicity

289 d components consisting predominately of the variable domain (VL) or the VL plus up to approximately

290 bodies typically recognize haptens using two variable domains (VL and VH), much less is known regardi

291 ediates the assembly of antibody light chain variable domains (VLs) into a correspondent symmetric te

292 Uniquely, the TIM-1 immunoglobulin variable domain was also required for P-selectin binding

293 Peptides from both conserved and variable domains were capable of inducing MIP-1alpha, MI

294 ibril formation of LEN, a benign light chain variable domain, were investigated at physiological pH i

295 o predicting the relative orientation of the variable domains when building homology models of antibo

296 oca: EpsH contains a large beta-sheet in the variable domain, where GspG contains an alpha-helix.

297 chains and light chain fragments composed of variable domains, which aggregate into amyloid fibers.

298 We show that the X-region represents a variable domain whose size changes with telomere length,

299 amin, however, Drp1 contains an unstructured variable domain, whose function is not yet fully resolve

300 trategy for the generation of human antibody variable domains with increased aggregation resistance.