ライフサイエンスコーパス: vasa

1 Saccharomycescerevisiae Ded1p and Drosophila Vasa.

2 egulating other multipotency factors such as vasa.

3 indle-class gene similar to the RNA helicase Vasa.

4 spermatogonia or spermatocytes and expressed VASA.

5 ated a human ortholog of the Drosophila gene vasa.

6 the germ cell markers nanos3, dnd, dazl and vasa.

7 n silencing, and perinuclear localization of Vasa.

8 ding modes and affinities for both Par-4 and VASA.

9 In many species examined, Vasa, a DEAD-box RNA helicase, is found in these morphol

10 In addition, both KGB-1 and CSN-5 bind Vasa, a Drosophila germ granule component; therefore, si

11 Genes encoding two zinc finger proteins, Vasa, a LIM domain protein, Sox and Jun-like transcripti

12 Although GLHs are homologous to Drosophila VASA, a polar granule component necessary for oogenesis

13 AMPPNP structure, and that of the Drosophila Vasa*AMPPNP*Mg2+*RNA complex, we targeted 20 positions i

14 er is nucleated by the DEAD box RNA helicase Vasa and contains the two Piwi proteins participating in

15 the regulatory sequence of medaka germ gene vasa and generated transgenic fish with visible GFP expr

16 By examining the expression of Strigamia vasa and nanos orthologues, we find that the primordial

17 ke two other known polar granule components, Vasa and Oskar, Aubergine remains cytoplasmic after pole

18 Using RNA interference we show that vasa and piwi are not required maternally or zygotically

19 lts add to the growing body of evidence that vasa and piwi can play important roles in somatic develo

20 us PGCs and absence of instructive roles for vasa and piwi in PGC formation are reminiscent of mouse

21 amine germ line development and the roles of vasa and piwi orthologues in the common house spider Par

22 These include vasa and piwi, which can play essential roles in any or

23 ess the germ-cell markers dazl, dnd, nanos3, vasa and piwil1 and the spermatogonial markers plzf and

24 script and protein expression patterns of Pt-vasa and Pt-piwi to show that primordial germ cells (PGC

25 e-related genes PRDM1, PRDM14, LIN28A, DAZL, VASA and SYCP3 induced direct conversion of somatic cell

26 ster transcripts immunoprecipitate with both Vasa and UAP56.

27 In Drosophila and Xenopus, Vasa and XVLP, respectively, are required for the establ

28 RNA pathway in regulating the levels of OSK, VASA, and possibly other genes involved in germline dete

29 In this study, we characterized vasa as a putative marker of germline precursors.

30 m cell-specific conditional knock-out (Cul4b(Vasa)),as well asCul4bglobal knock-out (Cul4b(Sox2)) mou

31 s developed normally and did not overexpress Vasa, as did embryos from a micromere deletion, implying

32 Vasa associates with the spindle and the separating sist

33 t association between WAGO-1 and a conserved Vasa ATPase-related protein RDE-12.

34 f mature germ cells, including expression of VASA, BOL, SCP1, SCP3, GDF9 and TEKT1.

35 and second-order originated from first-order vasa circumferentially around the vessel wall.

36 Here we show that the Vasa coding region is sufficient for its selective enric

37 We attempted to use the germline-expressed Vasa-Cre transgene to engineer a mouse mutation, but obs

38 w tool for genetic analysis of the germline, Vasa-Cre(ERT2), showed that this pathway functions throu

39 The Vasa DEAD-box helicases are widespread markers of germ c

40 The maternal RNAs vasa, dead end, nanos1, and daz-like all become localize

41 Furthermore, vasa deferentia from t-PA-null mice were hyporesponsive

42 MC proliferation in the central tail artery, vasa deferentia, seminal vesicles, prostate, and uterus,

43 tention of MD tissue in the epididymides and vasa deferentia.

44 Maelstrom protein is localized to nuage in a Vasa-dependent manner.

45 Interestingly, Maelstrom, but not Vasa, depends on two genes involved in RNAi phenomena, a

46 USTAVUS binding to the DEAD-box RNA helicase VASA (DINNNN).

47 stem cell population of the embryo, and that vasa expression in this embryo is restricted early by tr

48 on transplantation into fertile adults, thus vasa expression is correlated with the potential for gam

49 pleting maternal PIWI does not affect OSK or VASA expression or abdominal patterning but leads to fai

50 vasa expression was dynamic during asexual development i

51 In adults, vasa expression was observed in the gonads, as well as i

52 We show that ATP-dependent RNP remodeling by Vasa facilitates transfer of 5' sliced piRNA precursors

53 ms analyzed, Caenorhabditis elegans has four Vasa family members, the germline helicases GLH-1, GLH-2

54 In contrast, vasa from PAI-1-null mice were much more responsive (P<0

55 We therefore propose that UAP56 and Vasa function in a piRNA-processing compartment that spa

56 These results suggest that, when present, Vasa functions are essential to contributing to developm

57 In contrast to the single Vasa gene in most systems analyzed, Caenorhabditis elega

58 d adult tissues that expression of the human VASA gene is restricted to the ovary and testis and is u

59 sa gene, we find evidence for at least three vasa gene loci.

60 The vasa gene locus was duplicated from the original site an

61 a plasmid concentrations corresponded to the vasa gene, an important component of the nuage-piwi RNA

62 that Nile tilapia have a single copy of the vasa gene, we find evidence for at least three vasa gene

63 ed the zebrafish homologue of the Drosophila vasa gene, which, in the fly, encodes a germ-cell-specif

64 foundation for studying the role of multiple vasa genes in the development of tilapia gonads, and wil

65 stodes and trematodes have lost the piwi and vasa genes that are hallmark characters of the germline

66 nd sequencing of BAC clones for Nile tilapia vasa genes.

67 We discuss the evolution of the Vasa/GLH genes and current views of their functions and

68 Recent research suggests, however, that Vasa has a much broader function, including a significan

69 ing and export, whereas the DEAD box protein Vasa has an established role in piRNA production and loc

70 This absence of Piwi-like Agos and Vasa helicases prompts the question: how does the germli

71 The ATPase activity of Mouse Vasa Homolog (MVH) is essential for processing the inter

72 Furthermore, we find that the mouse Vasa homolog associates with Tudor domain-containing pro

73 Here, we report that mouse (mouse Vasa homolog), Xenopus laevis, and D. melanogaster Vasa

74 We have focussed on mouse vasa homologue (Mvh), a gene that is essential for male

75 Northern blotting revealed that zebrafish vasa homologue (vas) transcript is present in embryos ju

76 is necessary for the proper localization of Vasa, implying that Vasa is involved in the cyclin-depen

77 a previously hypothesized conserved role for vasa in cell cycle progression.

78 We identified that Vasa in the sea urchin is essential for: (1) general mRN

79 Here we identify vasa in two sea urchin species and analyze the regulatio

80 5 is required for the production of sDMAs of Vasa in vivo.

81 num homolog (Cf-vas) of the germ cell marker Vasa indicated that the B(4) blastomere in four cell-sta

82 We also learned that Vasa interacted with mRNAs in the perinuclear area and a

83 ll micromeres, whereas overexpression of the Vasa-interacting domain of Gustavus (GusDeltaSOCS) resul

84 l retardation protein (FMRP), dFXR, and VIG (Vasa intronic gene), through their association with RISC

85 Vasa is a broadly conserved ATP-dependent RNA helicase t

86 Vasa is a broadly conserved DEAD-box RNA helicase associ

87 Vasa is a conserved RNA-helicase found in the germ lines

88 Vasa is a DEAD-box RNA helicase that functions in transl

89 vasa is a highly conserved RNA helicase involved in anim

90 Vasa is a key germ cell determinant in many animal speci

91 Curiously, Vasa is also present in the somatic cells of many animal

92 The RNA helicase Vasa is another essential protein in germline developmen

93 germ cell genome is activated, we find that vasa is expressed specifically in germ cells.

94 e proper localization of Vasa, implying that Vasa is involved in the cyclin-dependent cell cycle netw

95 the cyclin-dependent cell cycle network, and Vasa is required for the efficient translation of cyclin

96 Results herein indicate that Vasa is utilized widely, and often induced transiently,

97 uced Gurken accumulation and modification of Vasa - is very similar to the phenotype of the spindle-c

98 Transient vasa knockdown did not have obvious effects on germline

99 ing the maternal piwi dose increases OSK and VASA levels correspondingly and doubles and triples the

100 d Xenopus laevis; one example is the Xenopus Vasa-like protein (XVLP) [4-6].

101 ariant Rhino, that nuage granules containing Vasa localize directly across the nuclear envelope from

102 to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs).

103 cells determined in early development, that vasa may function in an early stem cell population of th

104 in a profound heterochrony, suggesting that vasa might play a homeostatic role in asexual developmen

105 elstrom as a spindle-class gene that affects Vasa modification.

106 We found that maternally deposited vasa mRNA segregates early in development to a posterior

107 In contrast to vasa mRNA, which is present uniformly throughout all cel

108 umulate Vasa protein even though they retain vasa mRNA.

109 mulates in only a subset of cells containing vasa mRNA.

110 An overlapping set of genes, including vasa, nanos and piwi, operate in both multipotent precur

111 includes the classic germline lineage genes vasa, nanos and piwi.

112 c neuropathy results from destruction of the vasa nervorum and can be reversed by administration of a

113 n nerve function and limited the recovery of vasa nervorum and nerve function.

114 ts from microvascular ischemia involving the vasa nervorum and suggest the feasibility of a novel tre

115 graftment of EPCs in nerves and particularly vasa nervorum and their paracrine effects.

116 re was a profound reduction in the number of vasa nervorum associated with marked endothelial cell ap

117 ere uniquely localized in close proximity to vasa nervorum, and a smaller portion of these EPCs were

118 these drugs, resulting in destruction of the vasa nervorum, and accordingly that the neuropathy could

119 urbations affect neurons, Schwann cells, and vasa nervorum, which are held to be the primary cell typ

120 acrophages) and the endothelial cells of the vasa nervorum.

121 Neither Pt-vasa nor Pt-piwi gene products are localized asymmetrica

122 ombination with the germ cell-specific mRNA, VASA (OSKMV).

123 B1 and SPSB4 bind strongly to both Par-4 and VASA peptides.

124 Knocking down Piwi1, Vasa, Pl10 or Ncol1 expressed by blastema cells inhibite

125 We show that a vasa/PL10 homolog is required for proliferation and main

126 ility to test the function of these putative VASA positive germ cells is limited, these results demon

127 Whereas Vasa presence is often indicated as a metric for germlin

128 A knockdown of Gustavus protein reduces both Vasa protein abundance and its propensity for accumulati

129 We find that vasa protein accumulates in only a subset of cells conta

130 ly throughout all cells of the early embryo, vasa protein accumulates selectively in the 16-cell stag

131 nes in isolated culture, including selective Vasa protein accumulation and transcriptional activation

132 has a conserved, positive regulatory role in Vasa protein accumulation during embryonic development.

133 egative cadherin each suggest that, although vasa protein accumulation in the small micromeres is fix

134 domain of Gustavus (GusDeltaSOCS) results in Vasa protein accumulation throughout the embryo.

135 Further, these cells do not accumulate Vasa protein even though they retain vasa mRNA.

136 , paraffin-embedded tissue and characterized VASA protein expression in human germ cells at various s

137 rated polyclonal antibodies that bind to the VASA protein in formalin-fixed, paraffin-embedded tissue

138 stricted to a few special cases, such as the Vasa protein in the fruit fly Drosophila.

139 inds the N-terminal and DEAD-box portions of Vasa protein independently.

140 The VASA protein is cytoplasmic and expressed in migratory p

141 he sea urchin Strongylocentrotus purpuratus, Vasa protein is enriched in the small micromeres despite

142 We found that Vasa protein is present in all blastomeres of the early

143 VASA protein is present in fetal and adult gonadal germ

144 Inhibition of Vasa protein synthesis interferes with proper chromosome

145 In this paper, I use immunodetection of Vasa protein to study germ cell development in the amphi

146 oved respond by significant up-regulation of vasa protein translation, followed by spatial restrictio

147 served germ plasm components, nanos mRNA and Vasa protein, revealed that germ plasm segregation is a

148 heir failure to retain Seawi transcripts and Vasa protein.

149 omolog), Xenopus laevis, and D. melanogaster Vasa proteins contain both symmetrical and asymmetrical

150 hese cells express germ cell markers such as vasa, pumilio and piwi, as well as sphingosine-1-phospha

151 movement through the walls of the ascending vasa recta (AVR) in the exposed papillae of 2-week-old S

152 ave suggested that the fenestrated ascending vasa recta (AVRs) drain the interstitial fluid in this l

153 ltage-operated Na+ conductance in descending vasa recta (DVR) pericytes isolated from the renal outer

154 soconstriction of outer medullary descending vasa recta (OMDVR) is modulated by adenosine, we examine

155 nsport across the outer medullary descending vasa recta (OMDVR).

156 Histologic fibrosis of the medullary vasa recta and cortical interstitium was seen, but glome

157 eases [Ca2+]i in pericytes of the descending vasa recta as part of its constrictor action and that th

158 ferentiating outer medullary tubules and the vasa recta bundle area.

159 Disruption of the endothelium of the vasa recta by perfusion with latex microspheres enabled

160 nd descending limb of Henle epithelia and in vasa recta endothelia.

161 in were both expressed on the endothelium of vasa recta in the renal medulla, the lymph node subcapsu

162 nd low urea reflection coefficient of UT3 in vasa recta may be important for the formation of a conce

163 disease who showed peritubular capillary and vasa recta thrombi and capillary basement membrane alter

164 ([NO]i) of pericytes and endothelium of the vasa recta were independently measured with the use of f

165 ase of [NO]i of 19+/-6 U in pericytes of the vasa recta when the vessels were adjacent to medullary t

166 In isolated vasa recta with intact endothelium, Ang II reduced [Ca2+

167 Pericytes of isolated vasa recta without surrounding mTALs showed a rapid peak

168 elium of glomeruli, peritubular capillaries, vasa recta, and the principal cells (epithelial) of coll

169 s demonstrated syntaxin-4 mRNA in glomeruli, vasa recta, connecting tubules, and thin descending limb

170 urea rapidly as they traverse the ascending vasa recta, thereby preventing loss of urea from the med

171 nt exchange between ascending and descending vasa recta, to enhance the cortico-papillary osmolality

172 helial damage in peritubular capillaries and vasa recta.

173 the vascular bundles of the outer medullary vasa recta.

174 -sensitive NO production in pericytes of the vasa recta.

175 porter in erythrocytes and kidney-descending vasa recta.

176 titium, and medulla including vessels in the vasa recta.

177 the outer medulla is expressed in descending vasa recta.

178 CAM-1 is prominent in the endothelium of the vasa recta.

179 l tubule, thin descending limb of Henle, and vasa recta; AQP2, AQP3, and AQP4 in the collecting duct;

180 nd mesangial cells, and tubules around adult vasa rectae express Ang-2.

181 as roles in maturation of both glomeruli and vasa rectae.

182 NPC)-like FG repeat domains are found in the VASA-related P-granule proteins GLH-1, GLH-2, and GLH-4

183 We show that pgl-1 and the glh (Vasa-related) gene family, which encode protein componen

184 Here, we report a mitotic function of Vasa revealed in the sea urchin embryo.

185 ponents, which include dead end, nanos1, and vasa RNAs, are initially present in a wide cortical band

186 These components assemble on the surface of Vasa's helicase domain, which functions as an RNA clamp

187 A VASA segment of Par-4 mediated its binding and degradati

188 Conversely, PAF, which contains this VASA segment, competitively bound to Fbxo45 and rescued

189 late of the mesenchyme blastula stage and Sp-vasa, Sp-nanos2, Sp-seawi, and Sp-SoxE transcripts are l

190 Also, in these injured arteries, the vasa spatial distribution was disrupted compared with no

191 Furthermore,Cul4b(Vasa)spermatozoa exhibited defective arrangement of axon

192 suggest an evolutionarily conserved role of Vasa that is independent of its function in germ line de

193 micromeres despite a uniform distribution of vasa transcript.

194 licase encoded by the "posterior" group gene vasa (vas) in control of localization of the mRNA encode

195 , we find that Bru interacts physically with Vasa (Vas), an RNA helicase that is a positive regulator

196 hetic axons embedded in a few arterioles and vasa vasora were recently shown to store tissue plasmino

197 espectively, P < 0.01) and in the density of vasa vasorum (1.84+/-0.05/mm2 vs. 4.73+/-0.24/mm2; respe

198 to neovascularization in the coronary artery vasa vasorum (VV).

199 ro-computed tomography techniques that image vasa vasorum anatomy in relation to the atheroma.

200 ed especially by an increase of second-order vasa vasorum and disorientation of normal vasa vasorum s

201 ansplanted islets received blood supply from vasa vasorum and had access to drainage through venous t

202 We observed more extensive vasa vasorum and intimal neovascularization in knockout

203 cytes and T lymphocytes, and the role of the vasa vasorum and surrounding perivascular adipose tissue

204 IP NCs arose directly from adventitial vasa vasorum and were anatomically and quantitatively re

205 nd with the development of vascular disease, vasa vasorum are known to develop.

206 Macrophages in the plaque and around vasa vasorum are reduced, but we detect no direct effect

207 onstrate that rPAI-1(23) treatment decreased vasa vasorum area and length, which was supported by mic

208 rPAI-1(23)-stimulated mechanisms that cause vasa vasorum collapse.

209 reconstructed confocal microscopy images of vasa vasorum demonstrate that rPAI-1(23) treatment decre

210 We observe vasa vasorum density correlates highly with the extent o

211 prevents the increase in VEGF expression and vasa vasorum density of coronary arteries in experimenta

212 Vasa vasorum density was higher in the HC group compared

213 lloon-injured coronary arteries, adventitial vasa vasorum density was increased (3.16+/-0.17/mm2 vs.

214 nned, and reconstructed, and quantitation of vasa vasorum density was performed.

215 Plaque size and vasa vasorum density were compared to 2 controls: mice f

216 a and disarray, and stenotic arteries in the vasa vasorum due to medial SMC proliferation.

217 ccount for the relative lack of intracranial vasa vasorum early in life.

218 culture adventitial fibroblasts (AdvFBs) and vasa vasorum endothelial cells (VVECs) from the adventit

219 ic cells and macrophages), progenitor cells, vasa vasorum endothelial cells and pericytes, and adrene

220 ctivity was found in luminal and adventitial vasa vasorum endothelium.

221 To inhibit growth of vasa vasorum in atherogenic mice and assess its effect o

222 Recent attention has focused on the role of vasa vasorum in atherosclerotic and restenotic coronary

223 The three-dimensional anatomy of the vasa vasorum in early coronary atherosclerosis is unknow

224 causes regression or collapse of adventitial vasa vasorum in hypercholesterolemic mice by stimulating

225 titate three-dimensional spatial patterns of vasa vasorum in normal and balloon injured porcine coron

226 ate the three-dimensional spatial pattern of vasa vasorum in normal and experimental hypercholesterol

227 ssessment of the therapeutic response of the vasa vasorum in patients with atherosclerotic plaque.

228 origin of plaque vasculature and the role of vasa vasorum in plaque growth.

229 We also studied the spatial growth of vasa vasorum in regions of neointimal formation.

230 Coronary arteries contain a network of vasa vasorum in the adventitia.

231 iew offers insight into the possible role of vasa vasorum in the development of intracranial vascular

232 These data demonstrate that formation of new vasa vasorum in vasculitis is regulated by inflammatory

233 arteries suggests that the formation of new vasa vasorum is determined by the nature of the immune r

234 ionally, neovascularization arising from the vasa vasorum may promote atherosclerotic plaque progress

235 es is accompanied by neovascularization from vasa vasorum microvessels extending through the tunica m

236 mplex layer of the vessel wall consisting of vasa vasorum microvessels, nerves, fibroblasts, immune c

237 mplex layer of the vessel wall consisting of vasa vasorum microvessels, nerves, fibroblasts, immune c

238 pport a role for the endogenous ET system in vasa vasorum neovascularization in early coronary athero

239 in (ET) receptor antagonism reduces coronary vasa vasorum neovascularization in experimental hypercho

240 , a promoter of adventitial inflammation and vasa vasorum neovascularization in experimental models o

241 hanistic role of the endogenous ET system in vasa vasorum neovascularization in hypercholesterolemia

242 Cs, in a process involving ET-1, to regulate vasa vasorum neovascularization occurring in the adventi

243 ed phases, the role of eccentric remodeling, vasa vasorum neovascularization, and mechanisms of plaqu

244 molecular mechanisms regulating adventitial vasa vasorum neovascularization, which occurs in the pul

245 ede "macrovascular endothelial dysfunction." Vasa vasorum neovascularization, with endothelial leakag

246 increased, suggesting a mechanistic role for vasa vasorum neovascularization.

247 n the coronary arterial wall as well as with vasa vasorum neovascularization.

248 gests that adventitial neovascularization of vasa vasorum occurs in experimental hypercholesterolemic

249 Normal vasa vasorum originated from the coronary artery lumen,

250 growth factor-2 as mediators associated with vasa vasorum proliferation.

251 es of vasa vasorum were defined: first-order vasa vasorum ran longitudinally parallel to the vessel a

252 CML was localized to aortic and aortic vasa vasorum smooth muscle but not to collagen or elasti

253 er vasa vasorum and disorientation of normal vasa vasorum spatial pattern.

254 After 12 weeks, coronary vasa vasorum structure was assessed by three-dimensional

255 the quantitative response of the adventitial vasa vasorum to balloon-induced coronary injury.

256 -vessel vasculitis affecting branches of the vasa vasorum tree.

257 The density of newly formed vasa vasorum was proportional to vessel stenosis (r = 0.

258 ntiangiogenic effect of TSP-1, the number of vasa vasorum was reduced in aortas from diabetic rats.

259 Two different types of vasa vasorum were defined: first-order vasa vasorum ran

260 In normal arteries, vasa vasorum were restricted to the adventitia, but in i

261 to support development and maturation of the vasa vasorum within varying plaque types.

262 on," which is composed of dysfunction of the vasa vasorum's endothelium as well as "microcellular end

263 dventitia, particularly within microvessels (vasa vasorum) but not in cells of the intima or media.

264 al nitric oxide synthase (due to ingrowth of vasa vasorum), neointima formation, and loss of smooth m

265 f helping detect and even grade intracranial vasa vasorum, and this may provide new insights into our

266 hat the coronary vessel wall, especially the vasa vasorum, as well as bone marrow-derived endothelial

267 In conclusion, rPAI-1(23) inhibits growth of vasa vasorum, as well as vessels within the adjacent pla

268 from the artery lumen and outer adventitial vasa vasorum, deposit proatherogenic plasma molecules, r

269 ix can be imaged, as can angiogenesis of the vasa vasorum, plaque inflammation, and fibrin deposits o

270 lammation; and to stimulate expansion of the vasa vasorum, which can act as a conduit for continued i

271 use ECs line the vascular epithelium and the vasa vasorum.

272 from the development of immature neointimal vasa vasorum.

273 complete tissue integration and formation of vasa vasorum.

274 osclerotic plaque and associated adventitial vasa vasorum.

275 s the proliferation and intimal extension of vasa vasorum.

276 ural changes, including the formation of new vasa vasorum.

277 ssels, with proportionally more second-order vasa vasorum.

278 etween VEGF/VPF immunostaining and extent of vasa vasorum.

279 Although the total number of vasa was increased after injury, the total vascular area

280 injury, the total vascular area comprised of vasa was significantly reduced in injured vessels compar

281 NA encoding the primordial germ-cell marker, vasa, was present for more than 30 days in embryo cells

282 sion of PGC markers nanos1 and TDRD7 but not vasa were down-regulated when dnd mutant proteins lackin

283 Two different types of vasa were found and classified as first-order or second-

284 he diameters of first-order and second-order vasa were smaller in normal compared with balloon-treate

285 SN5 mutations also cause the modification of Vasa, which is known to be required for Gurken translati

286 Furthermore, we show that the RNA helicase Vasa, which is required for nanos RNA localization, also