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3 ortance of the ratio of maternally regulated vegetal and animal transcription factor activities in in
5 doderm- and mesoderm-inducing signals to the vegetal and marginal zones of the pre-gastrula Xenopus l
6 during oogenesis ensures its inheritance by vegetal and not animal cells, and directs the differenti
9 ost alkyl substituted pyrazines include mild vegetal aromas, not necessarily undesirable for the cork
11 is now evident that patterning in the animal/vegetal axis also needs to be taken into consideration.
12 patterning the mesendoderm along the animal-vegetal axis and indicate that dorsal and ventrolateral
13 mbryo, including establishment of the animal-vegetal axis as it relates to formation of the dorsovent
14 l cell divisions that occur along the animal/vegetal axis beginning early in embryogenesis, but not a
15 avage planes do not coincide with the animal-vegetal axis but rather form approximately 45 degrees of
16 the embryo and away from the initial animal-vegetal axis defined by the starting location of the bla
18 asymmetries are established along the animal-vegetal axis during oogenesis, but the underlying molecu
22 patterning because they subdivide the animal-vegetal axis into tiers of cells with different developm
24 e animal kingdom establish a primary, animal-vegetal axis maternally, and specify the remaining two a
25 s must be tightly regulated along the animal-vegetal axis of the early sea urchin embryo to allow bet
29 ectoderm-endoderm boundary along the animal-vegetal axis of the sea urchin embryo remain largely unk
37 of polar body formation sets up a new animal-vegetal axis that organizes cleavage and the segregation
38 pendent processes operating along the animal-vegetal axis, as evidenced by an expansion of SpNK2.1 ex
39 n of 2- to 4-cell divisions along the animal-vegetal axis, can affect the second step, the establishm
40 is established at right angles to the animal-vegetal axis, in contrast to hemichordates and indirect-
41 derm boundary more animally along the animal-vegetal axis, whereas expression of a dominant negative
42 PK) activation is polarized along the animal/vegetal axis, with the Xnr2-expressing cells in the vege
47 s made evident by the expression of Sox17 in vegetal blastomeres and Brachyury (Xbra) in marginal bla
48 n, beta-catenin accumulates in the nuclei of vegetal blastomeres and controls endomesoderm specificat
50 ipped orthologue expressed very early in all vegetal blastomeres and then gradually shifting to veg(1
51 ion of endogenous VegT and/or Vg1 in ventral vegetal blastomeres can induce a neural fate, but only a
52 t to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provide
53 The mesendoderm (ME) cells are the two most vegetal blastomeres in the early developing embryo of th
54 lium that undergoes epiboly and in the large vegetal blastomeres that gradually become internalized.
55 ntal defects of chimaeras made from the most vegetal blastomeres that result from later second cleava
65 his factor suppresses differentiation of all vegetal cell types, a phenotype that is very similar to
66 the region of xCR1 mRNA sufficient to confer vegetal cell-specific repression contained both Pumilio
69 red for the stabilization of beta-catenin in vegetal cells and provide evidence that Dsh undergoes a
70 the endogenous mesoderm inducing activity of vegetal cells before gastrulation; and third, it has sub
71 cient to impair sorting of animal cells from vegetal cells but is not sufficient (at similar doses) t
74 iption factor, is expressed in the yolk-rich vegetal cells of Xenopus embryos from the early gastrula
78 be the mesoderm-inducing signal released by vegetal cells, but its function in vivo has never been r
79 herited as a maternal mRNA localized only in vegetal cells, VegT activates the transcription of a lar
80 s, Vg1 and Bicaudal-C, are also inherited by vegetal cells, while germ plasm-associated mRNAs, such a
85 o obvious polarization yet, then the meiotic-vegetal center forms at zygotene bouquet stages, when sy
86 mRNA caused the release of Vg1 mRNA from the vegetal cortex and a reduction of Vg1 protein, without a
87 ther is not clear, but RNAs localized to the vegetal cortex during oogenesis are known to be essentia
89 required for localization of Vg1 mRNA to the vegetal cortex during the late RNA localization pathway.
92 Xenopus depends on rearrangements of the egg vegetal cortex following fertilization, concomitant with
98 otrimeric kinesin II becomes enriched at the vegetal cortex of stage III/IV Xenopus oocytes concomita
99 Prrp and Vg1 mRNAs are co-localized to the vegetal cortex of stage IV oocytes, substantiating an in
102 es mRNA and protein are both detected at the vegetal cortex of the oocyte; however, the protein is de
110 us laevis, several RNAs that localize to the vegetal cortex via one of three temporally defined pathw
111 Vg1 mRNA, VgRBP71 does not accumulate at the vegetal cortex with the mRNA; rather, it is present in t
112 dependent on VegT mRNA for anchoring to the vegetal cortex, indicating a novel function for maternal
113 lation of microtubules with plus ends at the vegetal cortex, supporting roles for these kinesin motor
120 ecular mechanisms governing the formation of vegetal cortical microtubule arrays are not fully unders
123 regions of the protein that are required for vegetal cortical targeting, including a phospholipid-bin
124 certified sulphur concentrations of various vegetal CRM samples applying linear calibration techniqu
125 iVegTR maternal RNAs become localized to the vegetal cytoplasm of fertilized eggs and are incorporate
126 enopus oocyte, Vg1 RNA is transported to the vegetal cytoplasm, where localized expression of the enc
127 pression at blastula can be activated by the vegetal determinants VegT and Vg1 acting in synergy with
130 unique opportunity to study mid-Pleistocene vegetal diet and is crucial for understanding subsistenc
131 est that RNA movement in both the animal and vegetal directions may influence the timescale of RNA lo
132 opose that SpKrl functions in patterning the vegetal domain by suppressing animal regulatory activiti
135 iptors of Malbec wines were aromas of cooked vegetal, earthy, soy and volatile acidity, as well as ac
136 ng of cell fates along the sea urchin animal-vegetal embryonic axis requires the opposing functions o
140 we provide three lines of evidence that two vegetal-enriched maternal factors (VegT, Vg1), which are
141 ryos, pigmented immunocytes are specified in vegetal epithelium, transition to mesenchyme, migrate, a
142 that the formation of mesoderm cells by the vegetal explants accounts for the apparent autonomous de
149 endent suppression of commitment of cells to vegetal fates and ectoderm differentiates almost exclusi
152 uphorbia resin, and possibly egg, wrapped in vegetal fibers, dated to approximately 40,000 BP, may ha
154 Smad1/5 linker region localizes to a ventral vegetal gastrula region that could coordinate DV pattern
155 gene is expressed maternally in an animal to vegetal gradient, and its expression levels decline rapi
158 mesoderm tissue specification process in the vegetal half of the early sea urchin embryo using Boolea
159 plasm, initially distributed throughout the vegetal half of the egg cortex, move to the vegetal pole
162 activity is uniformly distributed across the vegetal half of the Xenopus blastula and that this activ
163 al differentiation, while the ability of the vegetal half to regulate by forming apical lobe structur
166 cence in Xenopus oocytes was stronger in the vegetal hemisphere because of localization of internal s
167 ransplantation studies show that much of the vegetal hemisphere is competent to receive the induction
168 inductive interaction in which cells of the vegetal hemisphere of the embryo act on overlying equato
169 RNA localized to the mitochondrial cloud and vegetal hemisphere of the oocyte; second, it is required
170 meobox-containing genes are expressed in the vegetal hemisphere of the Xenopus embryo at the early ga
171 vity of a network of regulatory genes in the vegetal hemisphere, called the endomesoderm gene regulat
173 e 2.5C4-positive fibers are distributed in a vegetal (high) to animal (low) gradient on the basal sur
175 case of the equal-cleaving molluscs, animal-vegetal inductive interactions between the derivatives o
176 aying a role in the development of posterior/vegetal larval fates (i.e., endoderm) in C. lacteus.
177 rates the oral from aboral ectoderm; (3) the vegetal lateral CB, which bilaterally serves as signalin
179 ene that, due to its maternal expression and vegetal localization in Xenopus, has received close exam
180 t block this remodeling event also eliminate vegetal localization of the RNA, suggesting that RNP rem
181 mitted to endoderm through the action of the vegetal localized maternal transcription factor VegT.
184 between the progeny of animal micromeres and vegetal macromeres are established during the interval b
185 ives of the first quartet micromeres and the vegetal macromeres specify which macromere becomes the 3
186 axis, with the Xnr2-expressing cells in the vegetal marginal zone having no detectable activated MAP
187 in totipotent while surrounding cells in the vegetal mass become committed to endoderm through the ac
189 s signaling ligands that induce cells in the vegetal mass to form endoderm, and the marginal zone to
192 ndodermal genes was rescued in VegT-depleted vegetal masses by induction from an uninjected vegetal m
194 In the current study, we determined that the vegetal maternal dorsal determinant in fish is not the W
195 ur-cell stage blastomeres that inherits some vegetal membrane marked in the previous cleavage cycle t
196 ignaling through the Notch receptor from the vegetal micromere lineages diverts adjacent mesendoderm
197 , Mkif5Ba promotes formation of the parallel vegetal microtubule array required to asymmetrically pos
199 d became localized to the nuclei of the four vegetal-most cells at the 64-cell stage, which give rise
200 rms that Notch functions sequentially in the vegetal-most secondary mesenchyme cells and later in the
204 whereas 1-octen-3-one and one unknown spicy-vegetal odorant were highly correlated to the maturation
205 rmulation are powdered milk, peanuts butter, vegetal oil, sugar, and a mix of vitamins, salts, and mi
207 ssociated with mRNAs localized to either the vegetal or animal hemispheres, but was not found with co
208 lly serves as signaling centers; and (4) the vegetal oral CB, which delineates the boundary with the
209 lastomeres with the ability to function as a vegetal organizing center and to coordinate the developm
212 ation during gastrulation and overall animal-vegetal patterning at earlier stages of anthozoan develo
213 OX3c-injected Xenopus embryos, normal animal-vegetal patterning of mesodermal and endodermal markers
214 xamined the possible dorsoventral and animal-vegetal patterning roles for Nodal signals by using muta
215 en a transplanted micromere is placed at the vegetal plate after removing all 4 host micromeres, the
216 elopment for the activation of endo16 in the vegetal plate and for the activation of spec2a in the ab
220 vo transcripts, however, are enriched in the vegetal plate of the mesenchyme blastula stage and Sp-va
223 tion of the central mesodermal domain of the vegetal plate; and they exclusively give rise to the ske
224 roperties of 1d all depend on inheritance of vegetal polar lobe cytoplasm by its mother cell D at sec
227 thway, recruits endogenous kinesin II to the vegetal pole and colocalizes with it at the cortex.
228 nal blastomeres failed to migrate toward the vegetal pole and epiboly did not occur, a phenotype simi
229 were novel mRNAs over 4-fold enriched at the vegetal pole and six were over 10-fold enriched at the a
233 subset of the GRN connections in the central vegetal pole mesoderm of the late sea star blastula and
234 pe is rescued by axin mRNA injected into the vegetal pole of axin-depleted oocytes before fertilizati
239 vegetal half of the egg cortex, move to the vegetal pole of the egg, fusing with each other as they
241 enesis a maternal factor is localized to the vegetal pole of the oocyte that is a determinant of dors
243 Vg1 LE (VgLE) direct this transcript to the vegetal pole of Xenopus oocytes via the binding of a pro
245 hat factors colocalized with Vg1 mRNA at the vegetal pole relieve translational repression to allow e
246 al pole and the absence of micromeres at the vegetal pole results in the failure of macromere progeny
247 is to comprehensively interrogate animal and vegetal pole RNAs in the fully grown Xenopus laevis oocy
248 of sea urchin embryos four micromeres at the vegetal pole separate from four macromeres just above th
249 propose potential pathways operating at the vegetal pole that highlight where future investigations
250 dian, which runs from the animal pole to the vegetal pole through the center of Spemann's organizer,
251 eterminants, which are translocated from the vegetal pole to the future dorsal side of the embryo sho
252 ent of maternal dorsal determinants from the vegetal pole to the future dorsal side of the embryo, pr
253 ic mesoderm are co-expressed in the sea star vegetal pole, although this territory does not form a la
254 ong meridians running from the animal to the vegetal pole, both the formation of head structures and
255 s are selectively localized to the animal or vegetal pole, including determinants of somatic and germ
261 a-catenin levels, to adopt the fate of their vegetal-pole sisters, which normally have high nuclear b
262 age blastomeres from which the animal or the vegetal poles have been removed can develop into normal
263 in beta-catenin half-life at the animal and vegetal poles of the early embryo is sufficient to produ
264 doderm formation more animally, while in the vegetal portion, LvNotch signaling also promotes the ect
266 ow that Bmp signalling does occur within the vegetal prospective neural domain and that Bmp activity
267 find that Fgf activity is required to induce vegetal prospective neural markers and can do so without
268 etal region is the site of gastrulation; the vegetal region forms the ectoderm of the ventral and pos
269 rotein are localized to the Xenopus embryo's vegetal region from which the endoderm will arise and wh
270 lity to form apical lobe ectoderm, while the vegetal region has the ability to form a normal larva.
272 todermal covering of the apical lobe and the vegetal region is the site of gastrulation; the vegetal
274 evidence that LvDsh is active locally in the vegetal region of the embryo but is inactive in animal b
276 l blood islands and primitive blood from the vegetal region of the marginal zone in regularly cleavin
277 gest that endogenous maternal factors in the vegetal region repress the ability of blastomeres to for
278 o, and (2) a cell non-autonomous role in the vegetal region that regulates a signal(s) mediating ecto
280 e localized oocyte markers are expanded into vegetal regions in bucky ball mutants, but patterning wi
281 analyzed participation of kinesin motors in vegetal RNA transport and identified a direct role for X
287 sumptive endoderm is internalised as part of vegetal rotation, a large-scale movement that encompasse
289 certified reference materials and three real vegetal samples were employed for the quantitative deter
291 l embryo this protein suppresses the primary vegetal signaling mechanism that is required for specifi
292 onversely, SpKrl mRNA injection rescues some vegetal structures in beta-catenin-deficient embryos.
293 e zygote, is concentrated in both animal and vegetal teloplasm during stage 1 and is at higher levels
294 2/4, enhances differentiation of endoderm, a vegetal tissue, and promotes differentiation of cells ch
295 is known to be required for specification of vegetal tissues, because transcripts are undetectable in
296 rly cleavage stages, eomes is expressed in a vegetal to animal gradient in the embryo, whereas Eomeso
297 ractile ring of actin and myosin immediately vegetal to the blastoderm margin via Ca(2+) reduction or
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