ライフサイエンスコーパス: vesicle budding

1 complex, leading to coat polymerization and vesicle budding.

2 ulator of the late stages of clathrin-coated vesicle budding.

3 (COPI) to Golgi membranes to drive transport vesicle budding.

4 ctivation correlated with its enhancement of vesicle budding.

5 hese reagents inhibit later events in coated vesicle budding.

6 defective in GTP hydrolysis (Q71L) promoted vesicle budding.

7 mbrane, leading to membrane invagination and vesicle budding.

8 e polypeptides correlated with inhibition of vesicle budding.

9 we propose a minimal model of ESCRT-induced vesicle budding.

10 tic proteins cannot supply enough energy for vesicle budding.

11 n-coated invaginations resulting in impaired vesicle budding.

12 erspectives and discuss how ESCRTs may drive vesicle budding.

13 ing a role for phospholipid translocation in vesicle budding.

14 formation of highly curved membranes during vesicle budding.

15 olgi fragmentation in mitosis is mediated by vesicle budding.

16 transition between membrane constriction and vesicle budding.

17 rating and curvature-sensing proteins during vesicle budding.

18 rs the coat insufficiently stable to sustain vesicle budding.

19 ly disrupt membrane transport and inhibit ER vesicle budding.

20 unsaturated lipids, and are sites for coated vesicle budding.

21 with half-times of 10-20 s, independently of vesicle budding.

22 and Arf1 occur stochastically, even without vesicle budding.

23 napse has implicated endophilin in endocytic vesicle budding.

24 may participate in membrane deformation and vesicle budding.

25 the COPI coat complex, which is required for vesicle budding.

26 s critical for dynamin's ability to complete vesicle budding.

27 e same membrane and segregate at the time of vesicle budding.

28 cargo in modulating COPII coat assembly and vesicle budding.

29 f recombinant human ARF-1 enhanced secretory vesicle budding about 2-fold.

30 ully formed coated pit and immediately after vesicle budding, accumulation of a specific lipid can re

31 ility to function as a minimal machinery for vesicle budding agrees well with recent findings that al

32 In frog retinas vesicle budding also proceeds at 0 degrees C, both in vi

33 Vesicle budding and cargo selection are mediated by prot

34 hypothesize that this intracellular cycle of vesicle budding and fusion is an element of the active m

35 y in small transport vesicles by a series of vesicle budding and fusion reactions.

36 rated by realistic molecular rules governing vesicle budding and fusion.

37 in 1 (ASAP1) in the Golgi, which facilitates vesicle budding and Golgi exocytosis.

38 Consistent with their dual function in Golgi vesicle budding and homotypic fusion of vacuoles, approx

39 This is seen during vesicle budding and in the formation of microenvironment

40 brane anchors can target TyA-GFP to sites of vesicle budding and into EMVs, including: (i) a myristoy

41 Here we test the hypothesis that vesicle budding and membrane fusion are coupled by the i

42 ESCRT-II directly activates ESCRT-III-driven vesicle budding and scission in vitro via these structur

43 ssociation is dispensable for endosomal AP-3 vesicle budding and suggest that endosomal AP-3-clathrin

44 These and other results indicate that vesicle budding and trafficking may not be required for

45 nd that SV5 M protein alone could not induce vesicle budding and was not secreted from cells.

46 acids that are required for GLUT4 transport vesicle budding and/or fusion.

47 Membrane remodeling affects endocytosis, vesicle budding, and cargo selection.

48 eling events such as mitochondrial dynamics, vesicle budding, and cell division rely on the large GTP

49 cytosis, the sorting of membrane components, vesicle budding, and fission from the plasma membrane ar

50 ns, retain the endosomal property of outward vesicle budding, and serve as sites of immediate exosome

51 peroxisomal membrane proteins from the ER by vesicle budding, and the formation of nascent peroxisome

52 f cargo, suggesting that protein sorting and vesicle budding are functionally integrated.

53 a possible function for Ypt/rab proteins in vesicle budding as well.

54 Utilizing an in vitro vesicle budding assay, we demonstrate that Pho86p is req

55 ed by the addition of BAPTA to the cell-free vesicle budding assay.

56 also investigated using a coatomer-dependent vesicle budding assay.

57 Cell-free vesicle budding assays show that the F382L substitution

58 ctivity is required to promote Rab2-mediated vesicle budding at a VTC subcompartment enriched in recy

59 in a two-step process involving (1) outward vesicle budding at limiting membranes of endosomes (outw

60 lysis by dynamin is required to drive coated vesicle budding at the plasma membrane.

61 ould modulate both sialylation and secretory vesicle budding at the TGN.

62 ynchronized export of VSV-G stimulated COPII vesicle budding both in vivo and in vitro.

63 Thus, NO regulates endocytic vesicle budding by S-nitrosylation of dynamin.

64 Thus, the initiation of vesicle budding by Sar1p couples the generation of membr

65 ll stages of membrane trafficking, including vesicle budding, cargo sorting, transport, tethering and

66 Secretory vesicle budding (COPII) detected by the packaging of a S

67 h proteins and protein scaffolds involved in vesicle budding, cytoskeletal organization, and signalin

68 ulates the action of molecular chaperones in vesicle budding during endocytosis.

69 e a major class of coat proteins involved in vesicle budding during membrane transport.

70 well-known for its role in cargo sorting and vesicle budding from early endosomes, in most cases lead

71 gain insight into the mechanisms of synaptic vesicle budding from endosome-like intermediates, lysed

72 to a significant inhibition of general COPII vesicle budding from ER microsomes and the export of an

73 whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including

74 roteins were not active in cargo capture and vesicle budding from microsomal membranes.

75 (PKCiota/lambda) kinase activity to promote vesicle budding from normal rat kidney cell microsomes.

76 While Arf proteins are implicated in vesicle budding from the donor compartment, Ypt/rab prot

77 Vesicle budding from the endoplasmic reticulum (ER) empl

78 hannel functional expression at the level of vesicle budding from the ER and/or the local lipid envir

79 hilic protein that is required for transport vesicle budding from the ER in Saccharomyces cerevisiae.

80 Moreover, Ldgp63-containing COPII vesicle budding from the ER was inhibited by LdSar1:T34N

81 ns (components of a membrane coat that drive vesicle budding from the ER).

82 purified Sec proteins (COP II) required for vesicle budding from the ER.

83 In vivo, Sec14 activity is essential for vesicle budding from the Golgi complex.

84 nucleotide exchange factors (GEFs) initiate vesicle budding from the Golgi membrane surface by conve

85 e PITP, SCP-2 binds two ligands required for vesicle budding from the Golgi, PI, and fatty acyl CoA.

86 Sorting is achieved by vesicle budding from the ISGs, because it can be inhibit

87 The GTPase dynamin regulates endocytic vesicle budding from the plasma membrane, but the molecu

88 fied human PLD1 stimulated nascent secretory vesicle budding from the TGN approximately twofold.

89 ine metabolism might also regulate secretory vesicle budding from the TGN, we treated permeabilized r

90 nflicting data as to a role for dynamin-2 in vesicle budding from the TGN.

91 cruitment to facilitate AP-1/clathrin-coated vesicle budding from the TGN.

92 man ARF-1 (amino acids 2-17) also stimulated vesicle budding from the trans-Golgi network, in marked

93 been proposed to stimulate nascent secretory vesicle budding from the trans-Golgi network.

94 ranule fusion to plasma membranes, and small vesicles budding from granules.

95 g of anterograde cargo into coated transport vesicles budding from the ER [1].

96 PII machinery contribute to the formation of vesicles budding from the ER.

97 ansport mediated by four distinct classes of vesicles budding from the TGN.

98 Post-Golgi vesicles budding from the trans-Golgi network (TGN) are

99 avage of Hendra F occurs either in secretory vesicles budding from the trans-Golgi network or at the

100 omplex AP-3, which is associated with coated vesicles budding from the trans-Golgi network, and that

101 fuse with the cis-side and exit in transport vesicles budding from the trans-side.

102 coat assembly, membrane binding, and coated vesicle budding have provided detailed functional and st

103 proteins required to reconstitute transport vesicle budding in a cell-free reaction.

104 itates the recruitment of COPII proteins and vesicle budding in a reaction that is stimulated by Sar1

105 d that epsin is required for clathrin-coated vesicle budding in cells.

106 ve implications for the process of endocytic vesicle budding in general.

107 n of large nisin-Lipid II aggregates induced vesicle budding in giant unilamellar vesicles.

108 Surprisingly, vesicle budding into the endosome lumen occurs in the ab

109 Although COPII vesicle budding is promoted by GTP or a nonhydrolyzable

110 ar1p (the GTP-binding protein that initiates vesicles budding) is needed to package the membrane-asso

111 ction with integral membrane proteins of the vesicle budding machinery to ensure p24 packaging into t

112 ely recruited onto a specific organelle, and vesicle budding might be coupled to the presence of an a

113 involving ER-Golgi fusion followed by Golgi vesicle budding, mitotic cells were generated with fused

114 Rab GTPases regulate vesicle budding, motility, docking, and fusion.

115 at measures coat subunit assembly and coated vesicle budding on chemically defined synthetic liposome

116 using such membranes we have reconstituted a vesicle budding reaction dependent on the addition of CO

117 We also developed a cell-free COPII vesicle budding reaction that reconstitutes the capture

118 We developed a cell-free COPII vesicle budding reaction to examine SAC1 exit from the E

119 n purified COPII components were used in the vesicle budding reaction, Pma1p packaging was optimal wi

120 a cell-free coat protein complex II (COPII) vesicle budding reaction, that mutant TREM2 is exported

121 We developed a cell-free preperoxisomal vesicle-budding reaction in which Pex15Gp and Pex3p are

122 2p and how it contributes to clathrin-coated vesicle budding remain unclear.

123 wortmannin inhibits receptor sorting and/or vesicle budding required for delivery of endocytosed mat

124 Concurrently with vesicle budding, resident proteins are retained in the T

125 ted to other organelles are condensed at the vesicle budding site in the donor organelle, a process t

126 sicle recycling model that involves a single vesicle budding step mediated by clathrin and dynamin.

127 are closely connected with dynamin-mediated vesicle budding.This review summarizes current views in

128 ls that act positively and negatively during vesicle budding through a GTPase switch in the COPI coat

129 rebind to the ER to initiate a new round of vesicle budding unless it is dephosphorylated.

130 plore the role of clathrin in AP-3-dependent vesicle budding, using rapid chemical-genetic perturbati

131 of the four major steps in membrane traffic: vesicle budding, vesicle delivery, vesicle tethering, an

132 n a tensile force on the Golgi, required for vesicle budding via its interaction with an unconvention

133 Vesicle budding was dependent on temperature, cytosol, e

134 coat protein assembly and Sec16p-stimulated vesicle budding were explored with synthetic liposomes c

135 PLD1, designated Lys898Arg, had no effect on vesicle budding when added to the permeabilized cells.

136 ctor V and factor VIII to sites of transport vesicle budding within the endoplasmic reticulum lumen.