ライフサイエンスコーパス: vestibule

1 the electrical field of the channel (in the vestibule).

2 and in the flexibility of the extracellular vestibule.

3 and a radial expansion of the extracellular vestibule.

4 nd binding to a narrowing of the cytoplasmic vestibule.

5 o a cysteine introduced in the extracellular vestibule.

6 ccompanying changes of polarity of the sugar vestibule.

7 hair cells were in keeping with cells in the vestibule.

8 50 muM to 100 nM when the toxin occupied the vestibule.

9 GABA-recognition site but faces the channel vestibule.

10 e (S2) binding site within the extracellular vestibule.

11 ruitments and selectivity by the periplasmic vestibule.

12 takes place as the drug associates with the vestibule.

13 res at a position deep within the inner pore vestibule.

14 rved residues located at the entrance of the vestibule.

15 in the nonsensory regions of the cochlea and vestibule.

16 he S2 site, located within the extracellular vestibule.

17 tive linker sequences similarly fold in this vestibule.

18 tor muscles and reestablish the depth of the vestibule.

19 6 helix packing at the narrowest part of the vestibule.

20 n of caesium ions bound in the extracellular vestibule.

21 overall 2-fold symmetry and a large central vestibule.

22 ochlea but not in the one that surrounds the vestibule.

23 lvent-accessible at the extracellular domain vestibule.

24 meter of the protein, rather than inside the vestibule.

25 ntration of Ca(2+) ions in the extracellular vestibule.

26 t hairpins with 10-12 bp stems span the pore vestibule.

27 g a narrow constriction at the outer channel vestibule.

28 here one NH(4)(+) is captured in the binding vestibule.

29 above the macular sensory epithelium of the vestibule.

30 ansporter with a large (>45 A) extracellular vestibule.

31 A-wide channel, and a horn-shaped endofacial vestibule.

32 Cs, but significantly farther from the inner vestibule.

33 t maximum in the middle of the extracellular vestibule.

34 2 to a more protected position away from the vestibule.

35 esidues at the entrance to the intracellular vestibule.

36 contributes to the core of the extracellular vestibule.

37 the M3 segment in NR2C to the extracellular vestibule.

38 arboxyls and a lysine in the channel's outer vestibule.

39 lly localizing the C-11 sulfate in the outer vestibule.

40 on a Ca2+ binding site in the extracellular vestibule.

41 n the larval ectoderm region, but not in the vestibule.

42 formation about the topology of HERG's outer vestibule.

43 nner ear and is connected to the cochlea and vestibule.

44 and TM6a toward TM3 and TM8 to close the EC vestibule.

45 above the entrance to the selectivity-filter vestibule.

46 egion of the Kv voltage sensor, forms in the vestibule.

47 sence of calcium, the mobile unit closes the vestibule.

48 in binds to the N-terminal domain, opening a vestibule.

49 ubstrate or product dsDNA in the ion channel vestibule.

50 TRPA1 via a binding site not situated in the vestibule.

51 their Bodipy moiety within the M1 allosteric vestibule.

52 le for interaction with ligands entering the vestibule.

53 ween hydrophilic periplasmic and cytoplasmic vestibules.

54 9) lie respectively in external and internal vestibules.

55 rrow tunnel flanked by wider inner and outer vestibules.

56 ional modulation by membrane lipid and water vestibules.

57 xternal Na(+) binding opened a large aqueous vestibule (600 A(3)) leading to the sugar-binding site.

58 ee orthogonal semicircular canals, a central vestibule, a coiled cochlea, and an endolymphatic duct a

59 other TCAs, binds in an extracellular-facing vestibule about 11 A above the substrate and two sodium

60 allocation of the binding site to a luminal vestibule adjacent to Cys813 enclosed by part of TM4 and

61 a polymer is more likely to escape from the vestibule against the applied voltage gradient, while at

62 ibule but probably merging into the internal vestibule, allowing for control by the activation gate.

63 x region when it is captured in the alpha-HL vestibule, allowing the longer strand to translocate thr

64 esult from agonist binding to the allosteric vestibule alone, whereas the dualsteric binding mode pro

65 rnally accessible and that the external pore vestibule and activation gating of HCN channels are allo

66 ptor by substituting several residues in the vestibule and adjacent regions from the rat receptor to

67 ting cells and hair cells of the cochlea and vestibule and also to cochlear hair cell nuclei and ster

68 rences in the conformation of the outer pore vestibule and associated glutamate gate may account for

69 ional fluctuations of the polymer inside the vestibule and beta-barrel compartments of the protein po

70 Translocation through the extracellular vestibule and binding of leucine in the leucine transpor

71 hese hair cells are disorganized in both the vestibule and cochlea.

72 stablish the orientation of STX in the outer vestibule and confirm the clockwise arrangement of the c

73 cked into a domain that connects the central vestibule and corner clamp region of RyR, resulting in a

74 tions of hydrophilic residues exposed in the vestibule and identified a cluster of charged residues a

75 murine ortholog, early in development in the vestibule and in hair cells and supporting cells of the

76 Lmo4 resulted in the dysmorphogenesis of the vestibule and in the absence of three semicircular canal

77 to a common site located within the internal vestibule and inhibits cardiac Na+ channels by blocking

78 Two of these, the vestibule and intermediate sites, block (antagonize) the

79 ate the QX path without disturbing the outer vestibule and its selectivity function.

80 electrophoretically captured in the alpha-HL vestibule and positioned at the latch region, can be det

81 ibule by MTS reagents that enter the channel vestibule and react with the cysteine residues at vestib

82 , we propose a structural model of the outer vestibule and selectivity filter of the pore of the Ca2+

83 g triggers an expansion of the extracellular vestibule and stabilization of the open channel pore.

84 ained by contact points between hERG's outer vestibule and the bound BeKm-1 toxin molecule deduced fr

85 The large extracellular vestibule and the small-scale conformational changes cou

86 our external fenestrae to enter the rotundal vestibule and then cross one of four internal windows in

87 ly, (b) receptor-bound VTD lies in the inner vestibule, and (c) VTD blocks this mutant channel as a b

88 zed in a more external position in the outer vestibule, and does not bind via direct coordination wit

89 he extracellular gate, open an intracellular vestibule, and largely disrupt the two sodium sites, thu

90 crease in hydrophobic side chains lining the vestibule, and this was reflected in solvation of the ch

91 tances to current flow in the baths, channel vestibules, and selectivity filter to change differently

92 eraction, and allowed ssDNA to reside in the vestibule approximately 100 times longer than the first

93 c antidepressants (TCAs) in an extracellular vestibule approximately 11 A above the bound leucine and

94 matic residues in the receptor extracellular vestibule, approximately 15 A from the classical, 'ortho

95 ing-related structural dynamics at the outer vestibule are not well understood.

96 lthough the structural dynamics of the outer vestibule are significantly altered during activation an

97 ents associated with establishing PCP in the vestibule are unclear, hindering data interpretation and

98 253), and a salt bridge in the extracellular vestibule (Arg-30 and Asp-404).

99 minimizing deformation of the extracellular vestibule as the channel opens.

100 mutations in the dimer interface and channel vestibule as well as receptor composition.

101 stimulated by intense noise (middle ear and vestibule) as it was absent in CD1 mice with selective c

102 ubstitution of residues in the intracellular vestibule at positions 437, 438, 443, or 446 significant

103 Surprisingly, association with this vestibule, at a distance of 15 A from the binding pocket

104 ng liquid lidocaine compresses to the vulvar vestibule before penetration.

105 The cytoplasmic densities form a vestibule below the transmembrane domain with the C term

106 Four (out of eight) residues in the vestibule bound the dye, suggesting its role in substrat

107 only an inhibitor entry path to the luminal vestibule but also a channel leading to the ion binding

108 ive ion-conducting pore, bypassing the outer vestibule but probably merging into the internal vestibu

109 cupying the selectivity filter and cytosolic vestibule, but not the inner chamber.

110 domain 1a and occlusion of the extracellular vestibule by extracellular loop 4.

111 interpreted as blockage of the intracellular vestibule by MTS reagents that enter the channel vestibu

112 gative electrostatic field shifted the outer vestibule carboxylate pK(a) into the physiological range

113 ward-occluded states, with the extracellular vestibule closed and the intracellular portion of transm

114 laryngeal movements contribute to laryngeal vestibule closure and upper oesophageal sphincter openin

115 te binding site located in the extracellular vestibule comprised of residues shown recently to partic

116 The duration of the vestibule configuration depends on polymer composition a

117 d voltage also increases the duration of the vestibule configuration.

118 elements, an extracellular and a cytoplasmic vestibule connected by an extended narrow pore or select

119 her, the pore walls lining the extracellular vestibule constrict during channel closure.

120 base-specific interactions of dsDNA with the vestibule constriction "latch", a previously unrecognize

121 We propose a model in which this vestibule controls the entry and efflux of agonists from

122 s formed by D4938 and D4945 in the cytosolic vestibule determine RyR ion fluxes.

123 r HeET-1, indicating that the early phase of vestibule development occurs autonomously; only later de

124 d to the conduction pathway within the outer vestibule did not directly contribute to the relevant lo

125 backbone carbonyl groups at the periplasmic vestibule direct NH4(+) to the conserved aromatic cage a

126 rroborating the conclusion that the internal vestibule does not harbor a gate.

127 Moreover, the outer vestibule does not significantly contribute to ion selec

128 otional restriction experienced by the outer vestibule during inactivation gating.

129 ating may occur either at or below the inner vestibule entrance or at the selectivity filter.

130 TRPV1 reports distances in the extracellular vestibule, equivalent to those observed in the apo TRPV1

131 Perturbing the conformation of outer vestibule/external pore entrance (by cysteine substituti

132 ng data interpretation and employment of the vestibule for PCP studies.

133 nosine binds transiently to an extracellular vestibule formed by ECL2 and the top of TM5 and TM7, pri

134 strate occlusion within a post-translocation vestibule formed by GluT1 cytosolic domains.

135 pathway is characterized by a flexible outer vestibule formed by the TM1-TM2 loop, which leads to a n

136 phenylethylamino group, binds in an extended vestibule formed from transmembrane regions 2 and 7 (TM2

137 of F107 and F215, separating the periplasmic vestibule from the hydrophobic lumen, flip open and clos

138 that active glucose accumulation within the vestibule generates water flows simultaneously with the

139 l negatively charged residues from the upper vestibule had no effect on zinc inhibition.

140 Moreover, this vestibule hairpin is consistent with a closed-state conf

141 e direction from which the duplex enters the vestibule if the stabilities of leading base pairs at th

142 The vestibule improves the conduction rate by 40% and 75% at

143 Cd(2+) binding deep within the intracellular vestibule in the open state.

144 the bilayer and is connected to a cage-like vestibule in the periplasm.

145 Mutations in the extracellular vestibule in the SYTANLAAF motif disrupt the inhibitory

146 g to sites 1 and 2 of a K+ channel becomes a vestibule in which ions can diffuse but not bind specifi

147 segment forms the core of the extracellular vestibule, including a deep site for trapping blockers.

148 by NR1, forms the core of the extracellular vestibule, including binding sites for channel blockers,

149 llular domains, with a funnel-like exofacial vestibule (infundibulum), followed by a 15 A-long x 8 A-

150 tion is to keep the structure of periplasmic vestibule intact.

151 siding at the periphery of the extracellular vestibule, interposed between extracellular loops 4 and

152 ults suggest that the AMPA receptor external vestibule is a viable target for new positive allosteric

153 The outer vestibule is an important structurally extended region o

154 -gated K+ (Kv) channels, access to the inner vestibule is controlled by a bundle crossing formed by t

155 ether a-go-go related gene's (hERG's) outer vestibule is critical for its channel function: point mu

156 The structural widening of the extracellular vestibule is directly coupled to the opening of the ion

157 nts at SERT and suggest that the role of the vestibule is evolutionarily conserved among neurotransmi

158 omain (TM2), although a segment of the outer vestibule is formed by residues of TM1.

159 embranous labyrinth, Nor-1 expression in the vestibule is limited to nonsensory epithelial cells loca

160 -go-related gene (HERG) channel, whose outer vestibule is unique in structure and function among volt

161 e inner ear, composed of the cochlea and the vestibule, is a specialized sensory organ for hearing an

162 conserved aromatic cage at the bottom of the vestibule (known as the Am1 site).

163 ier to ion flow and render the intracellular vestibule less splayed during channel opening in the pre

164 he voltage-gated sodium channel at the outer vestibule lined by P-loops of the four domains.

165 sed-blocked ion channel, a pyramidal central vestibule lined by residues implicated in binding ion ch

166 bule and react with the cysteine residues at vestibule-lining positions.

167 hydrophobic residues near the extracellular vestibule (local).

168 the allosteric binding in the extracellular vestibule located above the central substrate binding (S

169 ally (N- to C-terminus) only in a permissive vestibule located in the last 20 A of the tunnel.

170 seven additional humanizing mutations in the vestibule-located binding site of AChBP to improve its s

171 This modified channel vestibule may also explain the dominant-negative effect

172 en-bonding networks within the extracellular vestibule may facilitate the transmission of cooperativi

173 Opening and closing of the vestibule might regulate access of substrates to the car

174 -resolution crystal-structure analysis, pore vestibule modeling, and structure-guided protein enginee

175 ts leading to restricted access to the upper vestibule, movement in the ion conducting lateral portal

176 ntitative polymerase chain reaction in nasal vestibule, nasal turbinate mucosa, and peripheral blood

177 demonstrate that both residues lie in a wide vestibule near the mouth of the pump's ion pathway.

178 y attract cations, through fenestrations, to vestibules near the ion channel.

179 In this vestibule, NH(4)(+) loses one water of hydration, but th

180 esidues Ile-210-Ser-212 line a funnel-shaped vestibule of 20-25 A in diameter, which remains unchange

181 ns between short single-stranded DNA and the vestibule of a biological pore.

182 aller B-form duplexes (d = 2.0 nm) enter the vestibule of alphaHL, resulting in decreased current blo

183 nitially stabilized within the extracellular vestibule of Cys-loop receptors, and this stabilization

184 ucose exit from preloaded cells depletes the vestibule of glucose, making it hypotonic and thereby in

185 ErgTx binds to the outer vestibule of HERG but may not physically occlude the por

186 dentified two specific residues in the inner vestibule of K(Ca)2.3 (Ser507 and Ala532) that determine

187 thylammonium (TEA) binding site in the outer vestibule of K+ channels, and the mechanism by which ext

188 ed, SSRIs and TCAs bind in the extracellular vestibule of LeuT and act as non-competitive inhibitors

189 uctural reconfiguration of the extracellular vestibule of LeuT in which a "water gate" opens through

190 spontaneous transition of the extracellular vestibule of LeuT into an outward-open conformation.

191 hly conserved ring of charge in the external vestibule of mammalian voltage-gated sodium channels, un

192 nsight into the 3D architecture of the outer vestibule of NaV through a systematic structure-activity

193 uter vestibule residues shows that the outer vestibule of open/conductive conformation is highly dyna

194 at or near the limiting aperture within the vestibule of the alpha-hemolysin pore.

195 harged amino acids facing the ion permeation vestibule of the channel in question.

196 ly charged lysine residue (K95) in the inner vestibule of the channel pore.

197 ts it from reaching its receptor site at the vestibule of the channel pore.

198 F ([(125)I]HgTX(1)A19Y/Y37F) in the external vestibule of the channel was used to characterize each m

199 ighly conserved residue in the extracellular vestibule of the channel, as the major element responsib

200 t the mutation, occurring near the cytosolic vestibule of the channel, reduces CDI as one of its prim

201 latter two domains, which may form the outer vestibule of the channel, represents a single functional

202 NR3A form a narrow constriction in the outer vestibule of the channel, which prevents passage of exte

203 lpha-helix organization near the cytoplasmic vestibule of the channel.

204 e four superficial carboxylates in the outer vestibule of the channel.

205 tion in negative charge at the extracellular vestibule of the channel.

206 udes the ion-permeation pathway at the outer vestibule of the channel.

207 n of eight positive charges within the outer vestibule of the conduction pathway had no effect on the

208 Finally, the expanded luminal vestibule of the E2P model explains high-affinity ouabai

209 model for the open conformation of the outer vestibule of the hERG channel, in which the S5-P linkers

210 tached by the tetrameric NT to the cytosolic vestibule of the InsP3R pore.

211 the transmitter binding site and (2) in the vestibule of the ion channel near the level of the trans

212 ta subunit interface and gammaTyr-105 in the vestibule of the ion channel, with photolabeling of both

213 esent at a specific location in the external vestibule of the ion-conducting pore.

214 pre-formed binding site in the extracellular vestibule of the iperoxo-bound receptor, inducing a slig

215 common" allosteric site in the extracellular vestibule of the M1 mAChR, suggesting that its high subt

216 Here, the extracellular vestibule of the M2 muscarinic acetylcholine receptor (m

217 esthetic derivative QX-222 into the internal vestibule of the mammalian rNaV1.4 channel.

218 crystal structure and the model of the outer vestibule of the Na+ channel as structural templates, we

219 Electrostatic surface potentials in the vestibule of the nicotinic acetylcholine receptor (nAChR

220 d oral cavity (n = 46 [93%]) and pharynx and vestibule of the nose (n = 26 [53%]).

221 of the green mamba snake, binds in the outer vestibule of the pore and, like Ca2+, is a positive modu

222 h cysteine-substituted residues in the outer vestibule of the pore of ASIC1a.

223 se intersubunit disulfide bonds in the outer vestibule of the pore, Y424C-G428C transitions between t

224 ular "gating element" with structures at the vestibule of the pore.

225 the channel that contribute to the internal vestibule of the pore.

226 tions that an allosteric site located in the vestibule of the receptor offers an attractive target fo

227 transmitter binding site and the allosteric vestibule of the receptor protein.

228 te of action is located at the extracellular vestibule of the receptor's ion channel pore and is acce

229 A second site is located in the vestibule of the receptor, in a preexisting intrasubunit

230 y in an extended conformation in the folding vestibule of the ribosome yet ultimately emerges at the

231 s located within a Debye length of the outer vestibule of the SACs, but significantly farther from th

232 ansmembrane domains (TMD) from the cytosolic vestibule of the Sec61 channel into the lipid bilayer.

233 glutamine hypothesized to line the cytosolic vestibule of the skeletal muscle RyR (RyR1).

234 we propose an antagonist-binding site in the vestibule of the TRPA1 ion channel.

235 ligand (MRS2500) binds to the extracellular vestibule of this GPCR, whereas another (BPTU) occupies

236 peptide predicted to contribute to the outer vestibule of TrpC1 channels revealed that TrpC1 is local

237 tion that the TM5-TM6 linker forms the outer vestibule of TRPV1 channels and that AG489 is a pore blo

238 near-equal expression levels in cochlea and vestibule of wild-type and Va(J) mutants.

239 The vestibules of adult guinea pigs were lesioned with genta

240 ed quaternary amines suggests that the inner vestibules of Kv and SK channels share structural simila

241 Both vestibules of the channel are strongly electronegative,

242 c receptors, initially making contact with a vestibule on each receptor's extracellular surface.

243 a corresponding asymmetry with an attractive vestibule only at the periplasmic side.

244 m duplex (d = 2.4 nm) is unable to enter the vestibule opening of alphaHL on the cis side, leading to

245 oup of Arg-189 points toward the periplasmic vestibule, opening up the constriction to accommodate th

246 charged reagents indicate that a wide outer vestibule penetrates deep into the Na+,K+-ATPase, where

247 formation with water-accessible intrasubunit vestibules penetrating from the extracellular end all th

248 hibitor, traps nascent TMDs in the cytosolic vestibule, permitting detailed interrogation of an early

249 he selectivity filter, pore helix, and outer vestibule play a crucial role in gating mechanisms.

250 The results show that the periplasmic vestibule plays a crucial role in solute selectivity, an

251 a ring of negative charge within the central vestibule, poised to contribute to cation selectivity.

252 s a "gating-modifier": it binds to the outer vestibule/pore entrance of hERG and increases current am

253 bunit interfaces that create the cytoplasmic vestibule portals.

254 the R domain docked inside the intracellular vestibule, precluding channel opening.

255 eed, mutagenesis of selected residues in the vestibule reduces the allosteric potency of (S)-citalopr

256 idues for positively-charged residues in the vestibule region exhibited a factor of approximately 20

257 figuration where a polymer occupies only the vestibule region of the pore, though a few appear relate

258 nature of their interactions with the outer vestibule remains debatable, however.

259 of NH(4)(+) into the periplasmic recruitment vestibule requires only 3.1 kcal/mol of energy.

260 model of TRPA1 based on Kv1.2 to select pore vestibule residues available for interaction with ligand

261 nz-2-oxa-1,3-diazol-4-yl (NBD)-labeled outer vestibule residues shows that the outer vestibule of ope

262 direct coordination with any specific outer-vestibule residues.

263 tion of several residues in the hP2X2 middle vestibule resulted in dramatic changes in the potency of

264 hether the zinc-binding site lies within the vestibules running down the central axis of the receptor

265 ate that C42 faces either the cytoplasm or a vestibule section wide enough to allow spermine to pass

266 ted to be located in the large extracellular vestibule seen in the crystal structure.

267 These results show how the periplasmic vestibule selectively recruits NH4(+) to the Am1 site, a

268 n contrast to forward translocation from the vestibule side of the pore, backward translocation times

269 Direct infusion of zVAD into the vestibule significantly increased hair cell survival aft

270 hK but makes different interactions with the vestibule, some of which are less favorable than for nat

271 he influx is much greater, the extracellular vestibule, specifically the M3 segment and regions C-ter

272 sed as molecular calipers to probe the outer vestibule structure of K channels.

273 lementary information about the unique outer vestibule structure of the HERG channel.

274 ack of effect on HC-030031 inhibition by the vestibule substitutions suggests that this molecule inte

275 similarity in pore architecture and aqueous vestibules, suggesting that there are unanticipated yet

276 el; it also reveals a low energy periplasmic vestibule suited for efficient uptake of glycerol from t

277 xtracellular surface through a deep and wide vestibule that emanates from a narrower pathway between

278 is used to identify residues on hERG's outer vestibule that interact with specific residues on the in

279 cket for both modulators to an extracellular vestibule that overlaps with a region used by orthosteri

280 and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4

281 Proximal to the exit port is a "folding vestibule" that permits the nascent peptide to compact a

282 ped hook region, colocated with the cochlear vestibule, that features the largest difference in fluid

283 Isolated ectoderm develops a vestibule (the precursor of adult ectoderm) and correctl

284 In the end of this recruitment vestibule, the phenyl ring of Phe107 dynamically switche

285 sket folds allowed them to enter the protein vestibule, the propeller fold exceeds the size of the la

286 by increasing the concentration of K+ in the vestibule through an electrostatic mechanism.

287 ngements that fully expose the intracellular vestibule to the cytoplasm.

288 s the solute transition from the periplasmic vestibule to the hydrophobic lumen in the Rh/Amt/MEP sup

289 tation of 180 degrees from the extracellular vestibule to the pore periplasmic side.

290 the exposure of extracellular and cytosolic vestibules to the bulk phase was evaluated as the reacti

291 row constriction near their apex with a wide vestibule toward the cytoplasm and an aqueous central ca

292 TSET modification of a cysteine in the outer-vestibule turret (Kv2.1 position 356/Shaker position 425

293 ng the binding site toward the extracellular vestibule (Tyr-108 and Phe-253), and a salt bridge in th

294 ate that Glu66 and the prolines in the outer vestibule undergo large fluctuations, which are modulate

295 more superficial parts of the extracellular vestibule, undergo extensive remodeling during channel c

296 by EPR, we show that, on average, the outer vestibule undergoes a modest backbone conformational cha

297 ing sites at the pore axis and extracellular vestibule, we propose a Ca(2+) permeation mechanism.

298 is perched on top of the US28 extracellular vestibule, whereas its amino terminus projects into the

299 utative nascent chain mimic, the cytoplasmic vestibule widens, and a lateral exit portal is opened th

300 polar pocket accessed from the extracellular vestibule with an important role for Asp-101.

301 The vestibule within the central pore of Na(+)-dependent cot