ライフサイエンスコーパス: viral replicase

1 d p92 that is needed for the assembly of the viral replicase.

2 t N interacts with nsp3a, a component of the viral replicase.

3 ch then inhibits the normal functions of the viral replicase.

4 by stimulating plus-strand synthesis by the viral replicase.

5 mature nonstructural proteins that form the viral replicase.

6 tion proteins as well as the assembly of the viral replicase.

7 Cdc34p is a novel component of the purified viral replicase.

8 (i) It binds to the viral replicase.

9 us is believed to be an integral part of the viral replicase.

10 itis C virus (HCV) is a key component of the viral replicase.

11 ructure is required for RNA synthesis by the viral replicase.

12 the site of replication and assembly of the viral replicase, activities that are mediated by cis-act

13 ponents of VRCs or VRCs in toto, we isolated viral replicase- and VRC-enriched fractions from TMV-inf

14 Positive strand viral replicases are membrane-bound complexes of viral a

15 Purified recombinant viral replicases are useful for studying the mechanism o

16 To understand this mechanism, a viral replicase assay that utilizes extracts from dengue

17 corresponding genome counterpart to provide viral replicase (B1+B2+B3/FCP and F1+F2/BCP) resulted in

18 lication, indicating that Xrn1 decay and the viral replicase compete to set RNA abundance within infe

19 irst, the minus strand is synthesized by the viral replicase complex (VRC), which then serves as a te

20 tion of several co-opted host factors in the viral replicase complex (VRC).

21 replication proteins and is recruited to the viral replicase complex (VRC).

22 in membrane-bound structures containing the viral replicase complex (VRC).

23 viruses are replicated by the membrane-bound viral replicase complex (VRC).

24 n proteins and inhibited the assembly of the viral replicase complex and viral RNA synthesis in vitro

25 facilitate the recruitment of GAPDH into the viral replicase complex in the yeast model host.

26 ating pyruvate kinase (PK) directly into the viral replicase complex to boost progeny RNA synthesis.

27 f viral RNA recruitment, the assembly of the viral replicase complex, and viral RNA synthesis.

28 f replication and assembly of the functional viral replicase complex.

29 is also necessary for assembly of an active viral replicase complex.

30 seems to be critical for the assembly of the viral replicase complex.

31 cation by interacting with components of the viral replicase complex.

32 nfected cells is believed to be catalyzed by viral replicase complexes (RCs), which may consist of va

33 We demonstrate that the in vitro assembled viral replicase complexes (VRCs) in artificial PE vesicl

34 ase (RdRp) and regulation of the assembly of viral replicase complexes (VRCs).

35 slation, some are recruited to improvise the viral replicase complexes for genome multiplication, and

36 ) replicase, we affinity purified functional viral replicase complexes from yeast.

37 and RNA viruses, replicate in membrane-bound viral replicase complexes in the cytoplasm of infected c

38 virus replication occurs via the assembly of viral replicase complexes involving multiple viral and h

39 rus replication requires the assembly of the viral replicase complexes on intracellular membranes in

40 selectively recruit viral RNAs into cognate viral replicase complexes.

41 bilizing activities, is a major component of viral replicase complexes.

42 ring the production of new plus strands, the viral replicase displaces the old plus strand in the dsR

43 ons may impair recognition of the RNA by the viral replicase during an early step in negative-strand

44 may interact with each other and/or with the viral replicase during genome replication.

45 ead-through domain affects the regulation of viral replicase expression by altering the likelihood th

46 hat suppress translational initiation of the viral replicase gene in the wild-type genome, have been

47 an alternative reading frame overlapping the viral replicase gene.

48 o 20:1 ratio between p33 and p92(pol) in the viral replicase, (iii) the activity of the tombusvirus r

49 as low and that the in vitro assembly of the viral replicase in a cell extract was inhibited by the c

50 NA as a template for (+)RNA synthesis by the viral replicase is facilitated by recruited host DEAD bo

51 e found that neither RNA1 (which encodes the viral replicase) nor RNA2 (which encodes the capsid prot

52 Viral replicases of many positive-strand RNA viruses are

53 Cu(2+) ions on the in vitro assembly of the viral replicase, on the activity of the viral RNA-depend

54 oteins is fully nested within the ORF of the viral replicase P.

55 Thus, a component of the viral replicase plays an important role in the neuropath

56 ctivity designed to block translation of the viral replicase polyprotein was first confirmed by reduc

57 ain-like protease (PLP), which processes the viral replicase polyprotein, has deubiquitinating (DUB)

58 icant delay in proteolytic processing of the viral replicase polyprotein.

59 ses (PLPs), PLP1 and PLP2, which process the viral replicase polyprotein.

60 us replication by cleaving a site within the viral replicase polyproteins and also removes ubiquitin

61 ain-like protease (PL(pro)) that cleaves the viral replicase polyproteins at three sites releasing no

62 This result was noteworthy, since viral replicase proteins have seldom been described in d

63 istant membrane fractions, which contain the viral replicase proteins, in cells with replicating HCV.

64 A also resulted in aberrant agglomeration of viral replicase proteins, including NS5A, NS5B, and NS3.

65 anscript was amplified to high levels by the viral replicase, resulting in decreased viral production

66 fraction of the yeast extract, in which the viral replicase-RNA complex became RNase- and proteinase

67 lace in a membraneous fraction, in which the viral replicase-RNA complex was RNase and protease resis

68 ement for a trans-acting protein factor, the viral replicase subunit nsp1beta.

69 in to define the interactions needed for the viral replicase to complete synthesis of viral RNA.

70 ion between N and the largest subunit of the viral replicase-transcriptase complex, nonstructural pro

71 the nonstructural protein 15 subunit of the viral replicase-transcriptase complex.

72 The viral replicase/transcriptase generated in SVCPC-infecte

73 ribonucleotides by abortive synthesis by the viral replicase using the 3' end of the viral genomic RN

74 e nature of the (-)RNA in the membrane-bound viral replicase, we performed complete RNA replication o

75 the putative RNA polymerase component of the viral replicase, were tested for their ability to suppor

76 ersion of several cellular proteins into the viral replicase, which otherwise play proviral roles in

77 RNA viruses [(+)RNA viruses] is performed by viral replicases, whose function is affected by many cel