ライフサイエンスコーパス: voltage clamp

1 ivalent cations under perfused two-electrode voltage clamp.

2 enopus oocytes, and studied by two-electrode voltage clamp.

3 s and ex vivo brain slices, using whole-cell voltage clamp.

4 effect on net ionic currents measured under voltage clamp.

5 , fura-PE3, to image Ca(2+) under whole-cell voltage clamp.

6 conducting channels determined by whole-cell voltage clamp.

7 ge steps to cells by simultaneous whole-cell voltage clamp.

8 pha(+) cells and activated SK currents under voltage clamp.

9 mbryonic kidney (HEK) cells under whole-cell voltage clamp.

10 selectivity under fixed conditions using the voltage clamp.

11 ransmembrane current (TMC) of the cell under voltage clamp.

12 ester (TMRM) using fluorescence imaging and voltage clamp.

13 low-voltage inactivation under two-electrode voltage clamp.

14 eriod; and steady-state INa inactivation via voltage clamp.

15 m cultured bag cell neurons under whole-cell voltage-clamp.

16 analogue were studied by using two-electrode voltage-clamp.

17 proteins, and NCX1 currents using whole-cell voltage clamping.

18 annel recording was performed after 72 hr by voltage-clamping.

19 In voltage-clamped (-40 mV) cells, H(2)S increased the freq

20 hDAT (human dopamine transporter) current in voltage-clamped (-60 mV) oocytes consistent with a DAT i

21 on was assayed using dual cell two-electrode voltage clamp after expression in Xenopus oocytes.

22 Whole-cell voltage-clamp analyses in HEK293 cells demonstrated that

23 ey-293 cells, and functions were assessed by voltage clamp analysis.

24 activation was quantified by Ussing chamber voltage clamp analysis.

25 Voltage-clamp analysis revealed that the NaV1.8/G1662S s

26 Voltage-clamp analysis showed a strong hyperpolarizing (

27 Our voltage-clamp analysis showed that R1279P depolarizes ch

28 ng of their functional properties comes from voltage-clamp analysis, the predominant approach for inv

29 ted the kinetic properties of the mutants by voltage clamp and (32)P uptake.

30 In this study, using whole cell voltage clamp and mechanical measurement techniques, we

31 n Xenopus laevis oocytes using two-electrode voltage clamp and on Cx43 and Cx46 expressed in HeLa cel

32 oocytes were characterized by two-electrode voltage clamp and optical osmotic swelling analyses.

33 is oocyte expression system by two-electrode voltage clamp and optical osmotic swelling assays.

34 responses were recorded by using 2-electrode voltage clamp and single-channel electrophysiology, wher

35 Experiments in voltage clamped and field stimulated ventricular myocyte

36 (LSO) from postnatal day 7 (P7) to P96 using voltage-clamp and auditory brainstem responses.

37 Using voltage-clamp and cAMP-protein kinase A (PKA) FRET senso

38 Using acutely isolated neurons, mixed voltage-clamp and current-clamp experiments were done at

39 Using whole-cell voltage-clamp and current-clamp recordings in acute hipp

40 Voltage-clamp and current-clamp recordings revealed that

41 in adult skeletal muscle fibers subjected to voltage-clamp and on RyR1 channel activity after incorpo

42 e isolated from CeA neurons under whole-cell voltage clamp, and their response to selective BK channe

43 urrents were recorded using 2-microelectrode voltage clamping, and surface expression was analyzed by

44 Combining multielectrode array, voltage-clamp, and current-clamp recordings, we assessed

45 T channels expressed in Xenopus oocytes were voltage-clamped, and distinct LRET signals were obtained

46 adients in the LUVs in the same manner as in voltage-clamped animal cells.

47 ively measured beyond the limitations of the voltage-clamp approach using fast Ca(2+) imaging with lo

48 of directional inhibition, as required for a voltage-clamp artifact.

49 Here, we test this voltage-clamp-artifact hypothesis in recordings from 62

50 us laevis oocyte-based automated 2-electrode voltage clamp assay.

51 We used a voltage-clamp assay on Xenopus oocytes injected with the

52 per CSF sample was performed in a whole-cell voltage-clamp assay.

53 sured in Xenopus oocytes using two-electrode voltage clamp assays.

54 an isophtalate (SBFI) AM from unperturbed or voltage-clamped astrocytes and respective glutamate tran

55 We probed Ca(2+) activation without voltage clamp by applying Na(+)-free (0 Na(+)) solution

56 rge and small alternans CaTs were applied to voltage-clamped cells.

57 Na(+) fluxes measured in vitro under voltage clamp conditions in controls demonstrate that bo

58 Under voltage clamp conditions, ATP activated large outward cu

59 Responses recorded under voltage-clamp conditions had identical short latencies a

60 Under voltage-clamp conditions in outside-out patches, this cr

61 validated on experimental data collected in voltage-clamp conditions using different techniques and

62 bed by a continuum electrostatic model under voltage-clamp conditions, the control of ion flow by the

63 lifetime of ASAP1, CAESR, and ArcLight under voltage-clamp conditions.

64 richia coli and in single HEK293 cells under voltage-clamp conditions.

65 RyR1 into planar phospholipid bilayers under voltage-clamp conditions.

66 mbrane potential in complex cells, since the voltage-clamp configuration constrains the membrane pote

67 rrent measurements using the Cut-open Oocyte Voltage Clamp (COVC) technique.

68 nd release assays in rat brain synaptosomes, voltage-clamp current measurements in cells expressing t

69 neuron, we compared simulation results under voltage-clamp, current-clamp and high [K(+)] membrane de

70 Here, using structural modeling, voltage-clamp, current-clamp, and multielectrode array r

71 Voltage clamp data were analyzed in order to characteriz

72 Together, these voltage-clamp data suggest that a number of release proc

73 nopus oocytes and tested using two-electrode voltage clamp, demonstrated an increase in ethanol sensi

74 pipettes to record whole-cell currents under voltage clamp, dopamine (30 mum) caused a reversible 64%

75 Well-coupled CTL myocytes are effectively voltage-clamped during Ca waves, protecting the heart fr

76 In whole-cell voltage clamp, Dyn-A opened G-protein-coupled inwardly r

77 We performed voltage clamp electrophysiological experiments to study

78 Xenopus oocyte expression and two-electrode voltage clamp electrophysiology, we found that co-expres

79 the Cys mutants was confirmed by whole-cell voltage clamp electrophysiology.

80 y and in Xenopus oocytes using two-electrode voltage clamp electrophysiology.

81 Xenopus oocytes and assayed by two-electrode voltage clamp electrophysiology.

82 m function were measured using two-electrode voltage clamp electrophysiology.

83 rologous expression systems using whole-cell voltage-clamp electrophysiology and immunohistochemistry

84 Using two-electrode voltage-clamp electrophysiology in Xenopus laevis oocyte

85 Using two-electrode voltage-clamp electrophysiology in Xenopus oocytes, oxyt

86 Using voltage-clamp electrophysiology of heterologously expres

87 Two-electrode voltage-clamp electrophysiology revealed that, when coex

88 Voltage-clamp electrophysiology studies of alpha1beta2M2

89 in Xenopus laevis oocytes and two-electrode voltage-clamp electrophysiology, and radiolabeled substr

90 Using two-electrode voltage-clamp electrophysiology, fast-application patch-

91 by depolarizing the membrane potential under voltage-clamp electrophysiology, TROX-1 inhibits Ca(V)2.

92 To overcome these voltage-clamp errors, we developed an approach to provid

93 Voltage clamp experiments showed activation of inward cu

94 In oocytes, two-electrode voltage clamp experiments showed that CYBDOM-mediated cu

95 neurons, 100 nm GxTX-1E broadened spikes and voltage clamp experiments using action potential wavefor

96 This finding is directly validated in voltage clamp experiments with Ca waves using isolated r

97 ecular dynamics, cysteine cross-linking, and voltage clamp experiments, we propose a dynamics-driven

98 ed by mutation of this residue combined with voltage clamp experiments.

99 GABAAR isoforms consistent with results from voltage-clamp experiments (EC50 values for alpha4beta3de

100 Voltage-clamp experiments demonstrate that 10 nM EGCG si

101 Biochemical and voltage-clamp experiments further demonstrated that Navb

102 Computational analyses and voltage-clamp experiments measuring L-type Ca(2+) curren

103 In voltage-clamp experiments, 2-AG reduced A-type potassium

104 activity using interleaved current-clamp and voltage-clamp experiments.

105 e in I(KS) channel function was confirmed by voltage-clamp experiments.

106 transmitter release, we combined presynaptic voltage clamp, fluorescence imaging, electron microscopy

107 annel recordings, cysteine accessibility and voltage clamp fluorimetry to probe the relationships bet

108 Using voltage-clamp fluorimetry and gating current analysis, w

109 Voltage-clamp-fluorimetry studies also indicated that in

110 Voltage clamp fluorometry (VCF) allows simultaneous meas

111 We used voltage clamp fluorometry (VCF) and molecular dynamics (

112 Voltage clamp fluorometry measurements combining electro

113 In this study, we use the voltage clamp fluorometry technique to identify the mole

114 We here used voltage clamp fluorometry to define how the homologous P

115 Here, we use voltage clamp fluorometry to determine how KCNE1 and KCN

116 Here, we use voltage clamp fluorometry to determine how KCNE3 affects

117 Here, we used voltage clamp fluorometry to investigate movements in th

118 Using voltage clamp fluorometry, we find that the acidic pocke

119 Using voltage clamp fluorometry, we found that the conformatio

120 orescence-labeled cysteines were measured by voltage clamp fluorometry.

121 pening/closing of the anion channel, we used voltage clamp fluorometry.

122 ent conformational changes were monitored by voltage clamp fluorometry.

123 gating in the absence of beta subunits using voltage-clamp fluorometry (VCF).

124 Using voltage-clamp fluorometry and UV photolysis of intracell

125 Patch-clamp and voltage-clamp fluorometry combine spectroscopic and elec

126 Voltage-clamp fluorometry experiments and kinetic modeli

127 Voltage-clamp fluorometry experiments indicate that both

128 Although voltage-clamp fluorometry fills this gap, it is limited

129 using the model ion channel, gramicidin, and voltage-clamp fluorometry measurements were performed wi

130 Voltage-clamp fluorometry showed a loss of a fast compon

131 We used voltage-clamp fluorometry to study conformational change

132 upled to changes in voltage sensing, we used voltage-clamp fluorometry to track conformational change

133 Voltage-clamp fluorometry was used to record ion channel

134 Here, we solved this problem by performing voltage-clamp fluorometry with a fluorescent unnatural a

135 Using an optical approach (voltage-clamp fluorometry) to track the movement of the

136 Using voltage-clamp fluorometry, we here detect two conformati

137 onformational changes within each VSD, using voltage-clamp fluorometry.

138 rophysiology, site-directed mutagenesis, and voltage-clamp fluorometry.

139 We recorded I(h) magnitude and properties by voltage clamp from dorsal root ganglion (DRG) neurons in

140 Conductance through single, voltage-clamped fusion pores directly reported sub-milli

141 is of the capacitive currents obtained under voltage clamp in molecular layer interneurons of juvenil

142 Here, we reveal that voltage clamp is completely ineffective for most excitat

143 o the membrane (confocal imaging, whole-cell voltage-clamp, K5fluo-4 as Ca(2+) indicator).

144 w (tau approximately 350 ms) tail current in voltage-clamped light responses and show that it is medi

145 light pulse duration, the typically reported voltage-clamp-measured ChR2 current traces are often not

146 Here we used two-electrode voltage clamp measurements in Xenopus oocytes together w

147 cement of delayed rectifier K(+) currents in voltage clamp measurements observed at least 3 h followi

148 in combination with confocal microscopy, and voltage-clamp measurements of hyperpolarizing currents,

149 sed by Ca(2+) imaging, (86)Rb(+) efflux, and voltage-clamp measurements.

150 We used the 2-electrode voltage-clamp method to quantify responses to acetylchol

151 In this study, using whole cell voltage clamp methodology, the actions of dexmedetomidin

152 high levels in plasma membrane and applying voltage clamp methods, Eriksen et al. (2016) have identi

153 w-pass filtering, whereas light responses in voltage-clamp mode produced bandpass filtering in all ON

154 quivalent to those expected to be imposed on voltage-clamped myocytes supported this hypothesis.

155 ld in LPP field potential studies but not in voltage clamped neurons; coupled with input/output relat

156 or, evoking no changes in holding current in voltage-clamped neurons and showing an IC50 of at least

157 EtOH increased the holding current of voltage-clamped neurons and this action was blocked by p

158 each of the mutant channels was assessed by voltage clamp of oocytes using micropipettes filled with

159 Voltage clamp of outside-out patches from L2/3 neurons r

160 Both computer simulation and in vivo dynamic voltage clamp of spinal motor neurons in septic rats wer

161 ) channel conductance measured by whole-cell voltage clamp of the same cell.

162 Using studies in both sperm and voltage clamp of Xenopus oocytes, we define a molecular

163 dvantages for biophysical studies, including voltage-clamp of both pre- and postsynaptic compartments

164 jecting PIP(2) (10 muM estimated final) into voltage-clamped oocytes stimulated NBC-mediated, HCO(3)(

165 solution imaging to track single vesicles at voltage-clamped presynaptic terminals of retinal bipolar

166 We measured these currents using a voltage-clamp protocol and then estimated the energetic

167 Application of voltage clamp protocols in the form of pre-recorded APs

168 ical electrophysiology, we recapitulate many voltage-clamp protocols and apply to Nav1.7, a channel i

169 ion channels typically involve sophisticated voltage-clamp protocols applied through manual or automa

170 ta recorded using standard electrophysiology voltage-clamp protocols that have not been developed wit

171 Voltage-clamp protocols were also used to determine whet

172 annotated metadata and responses to a set of voltage-clamp protocols, we assigned 2378 models of volt

173 g hyperpolarization preceding a depolarizing voltage-clamp pulse delayed the rise of the potassium co

174 Experiments were performed using Fluo-3 in voltage clamped rat ventricular myocytes.

175 We measured [Ca(2+)]i with fluo-3 in voltage-clamped rat ventricular myocytes.

176 2)2-nAChRs was confirmed using two-electrode voltage clamp recording of responses to nicotinic ligand

177 channel contribution to outward currents in voltage clamp recordings as determined by pharmacologica

178 Voltage clamp recordings from mEC stellate cells in rat

179 transmission was investigated in whole-cell voltage clamp recordings from medium spiny neurons of th

180 Voltage clamp recordings from SCs nucleated patches show

181 unds were evaluated using both two-electrode voltage clamp recordings from Xenopus laevis oocytes and

182 We made two-electrode voltage clamp recordings from Xenopus laevis oocytes exp

183 Using whole-cell voltage clamp recordings in brain slice preparations, th

184 In voltage clamp recordings in slices, a pH drop from 7.4 t

185 Whole-cell voltage clamp recordings of excitatory postsynaptic curr

186 In whole cell voltage clamp recordings of HEK293 cells, wild-type but

187 Synaptic measurements in whole cell voltage clamp recordings of inspiratory neurons revealed

188 Voltage clamp recordings revealed transient low voltage-

189 pressed in Xenopus oocytes and two-electrode voltage clamp recordings used to investigate the effects

190 In vitro intracellular current clamp and voltage clamp recordings were performed in muscle from a

191 Whole-cell voltage clamp recordings were then made from CA1 pyramid

192 t through them was measured using whole cell voltage clamp recordings.

193 Voltage-clamp recordings demonstrate a hyperpolarising s

194 Here, we perform voltage-clamp recordings from axial motoneurons in larva

195 Nonetheless, whole-cell voltage-clamp recordings from cdko rods revealed a profo

196 Whole-cell voltage-clamp recordings from cGOF or icGOF ventricular

197 Voltage-clamp recordings from dINs showed higher frequen

198 acute brain slices and performing whole-cell voltage-clamp recordings from individual dopamine neuron

199 Here we present voltage-clamp recordings from inner hair cells of the C5

200 assium current was measured using whole-cell voltage-clamp recordings from KF and locus coeruleus (LC

201 In the current study, in vivo whole-cell voltage-clamp recordings from layer 4 excitatory neurons

202 Using voltage-clamp recordings from nearby pyramidal cells, we

203 mission, we investigated Cplx function using voltage-clamp recordings from postsynaptic horizontal ce

204 4 function in photoreceptor ribbon synapses, voltage-clamp recordings from postsynaptic horizontal ce

205 Using whole-cell voltage-clamp recordings from rat calyx of Held presynap

206 Combined current- and voltage-clamp recordings from the same cell showed the s

207 Here, whole-cell voltage-clamp recordings from VB neurones of mouse thala

208 Here, whole-cell voltage-clamp recordings from VB neurones of mouse thala

209 Voltage-clamp recordings further revealed that although

210 Whole-cell voltage-clamp recordings further revealed that contrast

211 In voltage-clamp recordings in brain slices from adult mice

212 of Held, normally show strong depression in voltage-clamp recordings in brain slices.

213 Whole-cell voltage-clamp recordings in human embryonic kidney (HEK)

214 Whole-cell voltage-clamp recordings in small DRG neurons demonstrat

215 Whole-cell voltage-clamp recordings obtained after 3-7 days in cult

216 Whole-cell voltage-clamp recordings of cultured hippocampal neurons

217 We show in cut-open oocyte voltage-clamp recordings of gating and ionic currents of

218 e changes in grid spacing, we have conducted voltage-clamp recordings of I(h) in layer II stellate ce

219 Whole-cell voltage-clamp recordings of mEPSCs in CA1 pyramidal neur

220 Voltage-clamp recordings of miniature EPSCs (mEPSCs) fro

221 gic synapse--the calyx of Held--and combined voltage-clamp recordings of presynaptic Ca2+ currents (I

222 Two-electrode voltage-clamp recordings of Xenopus laevis oocytes expre

223 el suggesting that apparent DS excitation in voltage-clamp recordings results from inadequate voltage

224 Voltage-clamp recordings reveal that effective feedforwa

225 Whole-cell voltage-clamp recordings revealed a concomitant nonquant

226 Whole-cell voltage-clamp recordings revealed that blocking the ON p

227 Voltage-clamp recordings revealed that the Nav1.7-A1632G

228 Whole-cell voltage-clamp recordings revealed that tone-evoked synap

229 unds by means of Hille-Campbell Vaseline gap voltage-clamp recordings showed that the elongation of t

230 Voltage-clamp recordings showed that TNFalpha had no eff

231 In voltage-clamp recordings the outward current produced by

232 Whole-cell current- and voltage-clamp recordings were made from isolated guinea

233 Whole-cell voltage-clamp recordings were obtained from layer V pyra

234 ation condition and using whole-cell somatic voltage-clamp recordings, the amplitudes and kinetics of

235 y using in vivo whole-cell current-clamp and voltage-clamp recordings, we found that the synaptic inp

236 In this study, using in vivo whole-cell voltage-clamp recordings, we revealed eye-specific excit

237 rents is routinely achieved using whole-cell voltage-clamp recordings.

238 Whole-cell voltage clamping revealed that expression of CNNM3 influ

239 pus laevis oocytes followed by two-electrode voltage clamp showed that TcPho91 is a low-affinity tran

240 tions that determine the thermal motion of a voltage-clamped single-stranded DNA-NeutrAvidin complex

241 Under local dendritic voltage clamp, single-spine activation produced large sp

242 Voltage-clamp slow-ramp experiments showed that serotoni

243 inetics was limited by three factors: (1) HC voltage-clamp speed, (2) cone voltage-clamp speed, and (

244 actors: (1) HC voltage-clamp speed, (2) cone voltage-clamp speed, and (3) kinetics of Ca(2+) channel

245 Under voltage clamp, step depolarizations evoke transient Na(+

246 Furthermore, in two-electrode voltage clamp studies in Xenopus oocytes, both Ca(2+) an

247 Fluorescence assays and voltage clamp studies reveal that the self-assemblies in

248 co-expression of WT NDPK-A in two-electrode voltage clamp studies, but co-expression of a catalytica

249 ed CFTR conductance by AMPK in two-electrode voltage clamp studies.

250 Results of voltage-clamp studies revealed presynaptic defects chara

251 Further voltage-clamp studies revealed that ISO increases L-type

252 es using a Xenopus oocyte two-microelectrode voltage clamp system revealed mutations with only loss-o

253 Using the two-electrode voltage clamp technique and alpha4beta2 nAChRs in the Xe

254 s discovered by Hodgkin and Huxley using the voltage clamp technique in their landmark series of pape

255 ion channel function using the two-electrode voltage clamp technique on 16 cloned ion channels (12 K(

256 nopus laevis oocytes using the two-electrode voltage clamp technique showed GHB to be a partial agoni

257 the range of 1-780 nm) by the two-electrode voltage clamp technique using a standard Xenopus oocyte

258 Using the cut-open voltage clamp technique, we have simultaneously recorded

259 1) density was measured using the whole-cell voltage clamp technique.

260 e characterized using the two-microelectrode voltage clamp technique.

261 the current study, we used the two-electrode voltage-clamp technique alone or in combination with pH/

262 Ce1-mediated currents with the two-electrode voltage-clamp technique or pHi changes using Vm/pH-sensi

263 this study we have used the cut-open oocyte voltage-clamp technique to investigate the relationship

264 on whole oocytes, we used the two-electrode voltage-clamp technique, as well as pH- and voltage-sens

265 Using the two-electrode voltage-clamp technique, we examined metaflumizone inhib

266 s on GABAAR by means of a two-microelectrode voltage-clamp technique.

267 nels expressed in Xenopus oocytes, using the voltage-clamp technique.

268 s were measured using the two-microelectrode voltage-clamp technique.

269 3)(beta(2))(2) receptors using two-electrode voltage clamp techniques in Xenopus laevis oocytes indic

270 llular compartments, and (b) patch clamp and voltage clamp techniques, which investigate transporters

271 tory currents were recorded using whole cell voltage clamp techniques.

272 sing quantitative swelling and two-electrode voltage clamp techniques.

273 Voltage-clamp techniques have revolutionized clinical el

274 In this study, we use whole-cell voltage-clamp techniques to analyze light responses of i

275 To elucidate the mechanisms of IH, we used voltage-clamp techniques to investigate the [H]o, [Na]o,

276 Whole-cell voltage-clamp techniques were used to study the alterati

277 e therefore recorded lidocaine inhibition of voltage-clamped, tetrodotoxin-sensitive Na currents in m

278 sured in Xenopus oocytes using two-electrode voltage clamp (TEV) assays.

279 ed using cell-based assays and two-electrode voltage clamp (TEVC) technique on M2 channels, respectiv

280 identify the channel, we use whole-mitoplast voltage-clamping, the technique that originally establis

281 We used voltage clamp to characterize synaptic variability.

282 cted mutagenesis combined with two-electrode voltage clamp to investigate the role of the wrist domai

283 ogether, using Archaerhodopsin as an optical voltage clamp to provide the driving force for chloride

284 We used whole-cell voltage clamping to compare the biophysical parameters o

285 Furthermore, in hSERT-expressing oocytes voltage-clamped to -60 mV, APP(+) induced fluoxetine-sen

286 ile of the biological PD neuron, measured in voltage clamp, to constrain parameter values of a conduc

287 Voltage-clamp using ramp or repetitive action potentials

288 d a transient current beyond steady state in voltage-clamped ventricular myocytes as reported by othe

289 n Xenopus oocytes and the two-microelectrode voltage clamp was used to measure the kinetics and stead

290 Using ultrafast perfusion with voltage clamp, we applied glutamate to outside-out patch

291 Using two-electrode voltage clamp, we recorded from oocytes that were inject

292 Using single-electrode voltage clamp, we show that brief changes of ComInt 1's

293 Using whole-cell voltage-clamp, we have identified a typical M-current wi

294 tes expression system and two microelectrode voltage-clamp, we report the functional expression and t

295 live-cell immunofluorescence and whole-cell voltage-clamping, we found that modification of this cys

296 y at the plasma membrane, we used whole cell voltage clamp, Western blotting, and immunocytochemical

297 (mEPCs) and nerve-evoked EPCs (eEPCs) under voltage-clamp, which, unlike current-clamp records, were

298 currents in Xenopus oocytes using a cut-open voltage-clamp with extracellular solution titrated to ei

299 t produce detectable changes when studied by voltage-clamp within sympathetic neurons of the superior

300 opening voltage-gated calcium channels under voltage clamp, without affecting the number of synaptic