ライフサイエンスコーパス: water permeability

1 d NHDF-Ad fibroblasts (aquaporin-facilitated water permeability).

2 nd had approximately 12-fold reduced osmotic water permeability.

3 d calmodulin has been shown to regulate AQP0 water permeability.

4 volvement of AQPs 3, 4, and 5 in high airway water permeability.

5 s expected, they had reduced plasma membrane water permeability.

6 endothelia, where it increases cell membrane water permeability.

7 y reduced [Arg8]vasopressin (AVP)-stimulated water permeability.

8 uaporins (AQPs) are important in controlling water permeability.

9 ether, despite an up to 30-fold reduction in water permeability.

10 Isolated ZGs exhibit low basal water permeability.

11 , apical exocytosis, and increase of osmotic water permeability.

12 QP1-null mice despite a 20-fold reduction in water permeability.

13 or about 15% of total cholangiocyte membrane water permeability.

14 amino acid in facilitating the modulation of water permeability.

15 by a pollen-specific CPK that modifies their water permeability.

16 diates vasopressin-regulated collecting duct water permeability.

17 ght into the molecular mechanism of membrane water permeability.

18 ere also associated with an elevated osmotic water permeability.

19 among the laminated GO nanosheets for higher water permeability.

20 h the means to rapidly and reversibly modify water permeability.

21 ins in solute rejection with lower losses in water permeability.

22 d to the partial restoration of the membrane water permeability.

23 the molecular regulation of collecting duct water permeability.

24 iposomes up to 1:200 increased their osmotic water permeability.

25 Ildr1 significantly reduces the paracellular water permeability.

26 0 S/m), good NaCl rejection (>95%), and high water permeability.

27 iple factors have been found to affect their water permeability.

28 th-matching membrane mimetics could speed up water permeability.

29 s can impact GO membrane characteristics and water permeability.

30 s to a clear and selective increase in their water permeability.

31 ffecting AQP4 expression, array assembly, or water permeability.

32 may explain why the peptide seems to affect water permeability.

33 QP4 SNPs and showed that four nsSNPs reduced water permeability.

34 ions of the two polypeptides exhibit similar water permeabilities.

35 Lowering Ca(2+) increased water permeability 4.1 +/- 0.4-fold.

36 Diethylpyrocarbonate pretreatment increased water permeability 4.2 +/- 0.5-fold and abolished pH sen

37 cutoff of 255 Da, and a reasonably high pure water permeability (A) of 2.4 LMH/bar.

38 Regulation of AQP0 water permeability, a well-studied phenomenon in vitro,

39 ovel method for measuring osmotically driven water permeability across microvessels in intact lung.

40 To measure the osmotic water permeability across the microvascular endothelial

41 The increased water permeability after birth may facilitate the mainte

42 etion resulted in a > 4-fold reduced osmotic water permeability and > 2-fold reduced glycerol permeab

43 We reported increased water permeability and a low urea reflection coefficient

44 Here, we have characterized water permeability and amiloride effects in well differe

45 uaporin 5), which is required for regulating water permeability and cell volume.

46 in decreased transepithelial proximal tubule water permeability and defective fluid absorption.

47 AQP-1 enhances osmotic water permeability and FGF-induced dynamic membrane bleb

48 1 play a role in decreasing collecting duct water permeability and increasing water excretion, where

49 a membrane blebs, where it increases osmotic water permeability and locally facilitates the rapid, tr

50 posomes were found to have excellent osmotic water permeability and NaCl reflection coefficient value

51 o (PLR), and the addition of cholesterol) on water permeability and NaCl retention.

52 on of NF-kappaB prevented the changes in the water permeability and reflection coefficient to protein

53 The water permeability and regulation characteristics of eac

54 ation experiments revealed increases in both water permeability and salt passage as a result of oxida

55 udy the roles of aquaporin (AQP) in cellular water permeability and screen AQP-specific drugs.

56 Their high water permeability and solute rejection make them potent

57 e direct relevance to regulation of membrane water permeability and water homeostasis in epithelia of

58 red in spheroids of T47D breast cells (basal water permeability) and NHDF-Ad fibroblasts (aquaporin-f

59 reconstitution despite a 100-fold increased water permeability, and (c) CO2 blow-off by the lung in

60 In oocytes, AQP1-AQP1 exhibited high osmotic water permeability, and AQP1-C189M exhibited half activi

61 annels have been designed to mimic this high water permeability, and none reject ions at a significan

62 transparency, we compared corneal thickness, water permeability, and response to experimental swellin

63 secretory vesicles, AQP1-AQP1 exhibited high water permeability, AQP1-C189M exhibited half activity,

64 ross lipid bilayer membranes with a relative water permeability as high as 17 mum s(-1).

65 Water permeability assays in the cells were used to meas

66 Water permeability assays showed a high Pf in cells expr

67 re developed to quantify membrane and tissue water permeabilities at the ocular surface and to compar

68 ic environment, the apparent coefficient for water permeability at 21 degrees C varied modestly in a

69 um extracellular matrix that forms the major water permeability barrier in mammalian epidermis.

70 s of varying composition gave widely varying water permeabilities but similar CO2 permeabilities at 2

71 of the 34 kDa polypeptide does not influence water permeability but may contribute to membrane traffi

72 f these mutant channels showed a decrease in water permeability but not the expected increase in glyc

73 In constrast, AtNIP6;1 showed no measurable water permeability but transported glycerol, formamide,

74 AQP4 into proteoliposomes increased osmotic water permeability by >40-fold, giving a p(f) of 15 x 10

75 ositive control, 0.3 mM HgCl2 inhibited AQP1 water permeability by >95%.

76 but increased their plasma membrane osmotic water permeability by 5- to 10-fold.

77 These results suggest that TEA reduces AQP1 water permeability by interacting with loop E.

78 role in regulation of renal collecting duct water permeability by vasopressin.

79 le for non-muscle myosin II in regulation of water permeability by vasopressin.

80 ese results indicate that corneal epithelial water permeability can be regulated, presumably to prote

81 The high water permeability characteristic of mammalian red cell

82 a secretory agonist (forskolin), the osmotic water permeability coefficient (P(f)) decreasing up to 5

83 model was developed to determine the osmotic water permeability coefficient (Pf) of lung microvessels

84 In studies done at 10 degrees C, the osmotic water permeability coefficient decreased by 55% (p < 0.0

85 osmolarity-dependent manner with an osmotic water permeability coefficient of 66.4 x 10(-4) cm/s.

86 coefficients of mouse oocytes as well as the water permeability coefficient of golden hamster pancrea

87 The water permeability coefficient of the golden hamster pan

88 osmotic challenges and estimated the osmotic water permeability coefficient, P(f), to be approximatel

89 orm a water-filled beta-sandwich that induce water permeability comparable with channel-forming prote

90 It displayed approximately 10% average water permeability compared to WT AqpZ, which had been a

91 led with CAII demonstrated increased osmotic water permeability compared with ghosts resealed with al

92 and CF airway epithelia have relatively high water permeabilities, consistent with the isosmotic ASL

93 AQP0 reduces its osmotic water permeability constant (Pf) in response to increase

94 Expression of AQP1 and osmotic water permeability (control P(f) = 0.046 +/- 0.005 cm/se

95 Water permeability decreased by 65% in parotid and 77% i

96 sorption in distal nephron segments with low water permeability, diuretic agents that impair this mec

97 hat mediate the fine-tuning of cell membrane water permeability during development or in response to

98 s or in AQP5-expressing oocytes with osmotic water permeabilities equivalent to those seen with AQP1.

99 se results indicate very different intrinsic water permeabilities for the mammalian aquaporin homolog

100 stribution of channel water and an increased water permeability for the W48F/F200T mutant.

101 The low water permeability found in AQP1/UT-B null erythrocytes

102 putative D. discoideum AQPs, WacA, and AqpA, water permeability has not been shown.

103 expressing aquaporins have high or regulated water permeability; however, there has been no direct ev

104 opus laevis oocytes, AQP6 exhibits low basal water permeability; however, when treated with the known

105 We report that water permeability in 0.8-nanometer-diameter carbon nano

106 AQP5 is the major pathway for regulating the water permeability in acinar cells, a critical property

107 Water permeability in an AQP4-enriched brain vesicle fra

108 xplain physiological observations that found water permeability in AQP0 to be substantially lower tha

109 Whereas osmotic water permeability in AQP1-reconstituted liposomes was >

110 enic diabetes insipidus produced by impaired water permeability in collecting-duct basolateral membra

111 res, supporting the involvement of aquaporin water permeability in cytokine secretion.

112 We found reduced osmotic water permeability in freshly isolated DRG neurons from

113 rted here to measure osmotic and diffusional water permeability in intact airways.

114 Herein we map the water permeability in intact arteries, using coherent an

115 We developed novel methods to compare water permeability in isolated choroid plexus of wild-ty

116 AQP1 deficiency reduced plasma membrane water permeability in lens epithelium by 2.8 +/- 0.3-fol

117 Aquaporins increase the water permeability in many cell types across many specie

118 ds indicated that amiloride does not inhibit water permeability in non-cystic fibrosis (non-CF) or CF

119 In contrast, the water permeability in parotid and sublingual acinar cell

120 results provide the first functional data on water permeability in perinatal lung.

121 scattering showed high mercurial insensitive water permeability in plasma membrane vesicles.

122 Thus, the high water permeability in proximal tubule of wild-type mice

123 cell surface expression of AQP2 and osmotic water permeability in the absence of forskolin stimulati

124 Vasopressin antagonists, which promote low water permeability in the collecting ducts and, hence, f

125 The molecular determinants of water permeability in the human lung are incompletely de

126 chanism is sufficient to explain the reduced water permeability in the mutant without invoking effect

127 reases in vasopressin (AVP)-elicited osmotic water permeability in the terminal inner medullary colle

128 It has been controversial whether high water permeability in the thin descending limb of Henle

129 oM significantly inhibited or increased AQP1 water permeability in these assays.

130 er transport, we measured osmotically driven water permeability in vitro in microperfused OMDVR from

131 on decreased membrane AQP2 and AQP2-mediated water permeability in Xenopus oocytes expressing P2X(2),

132 g measurements indicated erythrocyte osmotic water permeabilities (in cm/s x 0.01, 10 degrees C): 2.1

133 crease in proteinuria, as well as glomerular water permeability, in the context of progressive deplet

134 Because the AQP4 M1 isoform exhibits greater water permeability, in this study, we explored the possi

135 quaporin-2 trafficking and forskolin-induced water permeability increase were blocked by F-actin disr

136 lation of apical aquaporin-2 trafficking and water permeability increase.

137 , the prominent changes in lysis tension and water permeability indicate that major changes occur in

138 None of the above altered the water permeability induced by AQP1 or AQP4.

139 Because the greatest change in AQP0 water permeability is in the normal pH range found in th

140 Water permeability is necessary but also insufficient.

141 equilibration, even when intrinsic membrane water permeability is not high.

142 AQP5-null mice indicates that high alveolar water permeability is not required for active, near-isos

143 evidence that high epithelial cell membrane water permeability is required for active, near-isosmola

144 Passive water permeabilities (Lp) of the cotransporters were det

145 Water permeability measured between the airspace and vas

146 Microvascular endothelial water permeability, measured by a related pleural surfac

147 Osmotic water permeability, measured by stopped-flow light scatt

148 Water permeability measurements indicated that functiona

149 er, we improved the signal-to-noise ratio of water permeability measurements on AQP0 proteoliposomes.

150 To define the fraction of membrane water permeability mediated by AQP1, red cells were obta

151 e plasma membrane and abolished the membrane water permeability mediated by transgenic PIP2;7.

152 reproduce and predict volume changes in high-water-permeability membranes under hypoosmotic gradients

153 vasopressin-dependent renal collecting duct water permeability; mutations or downregulation can caus

154 The channel (unit) water permeabilities of AQP4 measured by osmotic-gradien

155 ocytes expressing AQP2 cRNAs, single-channel water permeabilities of mutants L22V, T126M, and A147T w

156 The gas and water permeabilities of these nanotube-based membranes a

157 Here we test the hypothesis that the water permeabilities of two naturally occurring isoforms

158 llow fiber membrane exhibits an average pure water permeability of 3.2 L m(-2) h(-1) bar(-1) and show

159 wly developed membrane exhibited a high pure water permeability of 5.01 L m(-2) h(-1) bar(-1) and com

160 a fast time constant associated with osmotic water permeability of AQP-expressing cells and a slow ph

161 In Xenopus oocytes, the water permeability of AQP0 (P(f)) increases with removal

162 e additive effects and together increase the water permeability of AQP0 40-fold to a level comparable

163 d into liposomes are challenging because the water permeability of AQP0 is only slightly higher than

164 Our analysis suggests that the low water permeability of AQP0 may help maintain the mechani

165 nt environment, both may act to increase the water permeability of AQP0.

166 Previous experiments have shown that the water permeability of AQP4 depends on the cholesterol co

167 the known water channel inhibitor, Hg2+, the water permeability of AQP6 oocytes rapidly rises up to t

168 xtraordinary long intracellular loop induced water permeability of AqpB, hinting at a novel gating me

169 Lowering pH to 6.5 increased the water permeability of aquaporin (AQP0) 3.4 +/- 0.4-fold.

170 The water permeability of biological membranes has been a lo

171 The water permeability of both proteins was tested using the

172 the observation of the tremendously enhanced water permeability of carbon nanotubes, those iconic obj

173 ation was dominant, which in turn caused the water permeability of chlorinated membrane to decrease.

174 Osmotic water permeability of cortical collecting-duct basolater

175 In contrast, the corresponding water permeability of dendrimer-modified membranes decre

176 +) caused a two- to fourfold increase in the water permeability of endogenous AQP0.

177 of these vesicles was used to determine the water permeability of endogenously expressed AQP0.

178 Water permeability of G1-NH2 modified membrane decreased

179 ion was used to test mechanical strength and water permeability of giant-fluid bilayer vesicles compo

180 Glycerol and water permeability of GlpF both occur with low Arrhenius

181 traethylammonium (TEA) chloride, reduces the water permeability of human AQP1 channels expressed in X

182 pplied to measure the apical plasma membrane water permeability of intact toad urinary bladder; Pf in

183 ing on L(o), and its relationship to osmotic water permeability of isolated root cortex protoplasts,

184 The water permeability of liposomal membrane is found to be

185 .5 caused a two- to fourfold increase in the water permeability of mammalian AQP0.

186 A method is proposed to measure the water permeability of membrane channels by means of mole

187 oocytes showed that it increased the osmotic water permeability of oocytes 5-fold; this water transpo

188 ants, was found between Kros and the osmotic water permeability of protoplasts from the veins but not

189 The water permeability of SjAQP was inhibited by 1 mM HgCl2,

190 The water permeability of the fouled membranes was lowered b

191 ment of Sjogren's syndrome by increasing the water permeability of the gland to restore saliva flow.

192 demonstrated that vasopressin increases the water permeability of the inner medullary collecting duc

193 nine vasopressin (AVP) regulates the osmotic water permeability of the kidney collecting duct by indu

194 cell membrane and would account for the high water permeability of the lung.

195 nd thus contribute to the fine-tuning of the water permeability of the plasma membrane.

196 cm/s indicated a substantial single channel water permeability of UT-B of 7.5 x 10(-14) cm(3)/s, sim

197 We report that the water permeability of wild-type Escherichia coli during

198 a low but measurable enhancement in osmotic water permeability of Xenopus oocytes and hence represen

199 ted the effects of external pH and Ca(2+) on water permeability of Xenopus oocytes injected with aqua

200 of the mutant AQP1 channels showed that the water permeability of Y186F was equivalent to that of wi

201 However, in contrast to wild-type AQP1, the water permeability of Y186F was not reduced with 100 mic

202 Membrane water permeabilities (P(f)(mem)) of calcein-stained surf

203 Tissue water permeabilities (P(f)(tiss)) across intact cornea a

204 hile the M278T mutation increased normalized water permeability (P < 0.001).

205 d with WT), and no change in the diffusional water permeability (P(d)).

206 panied by a 2.8-fold increase in the osmotic water permeability (P(f) 280 +/- 40% compared with WT),

207 t scattering assays to have elevated osmotic water permeability (P(f) = 57 microm x s(-1) versus 12 m

208 , resulting in a approximately 5-fold higher water permeability (P(f)) across the blood-brain barrier

209 is an aquaglyceroporin with a modest osmotic water permeability (P(f)) and the ability to transport u

210 llecting duct, vasopressin increases osmotic water permeability (P(f)) by triggering trafficking of a

211 Osmotic water permeability (P(f)) in response to a 200 mM NaCl g

212 ses predict different values for the osmotic water permeability (P(f)) of airway epithelia.

213 Airspace-capillary osmotic water permeability (P(f)) was measured in isolated perfu

214 onate; net water movement (J(v)) and osmotic water permeability (P(f)) were then calculated.

215 accessible surface, channel gating dynamics, water permeability (p(f)), and a dihedral angle are defi

216 AQPxlo expression increased osmotic water permeability (P(f)), as well as the uptake of glyc

217 The AQP4 single channel water permeability (p(f)), estimated from Pf and protein

218 evels and decreased mesophyll and BS osmotic water permeability (P(f)), mesophyll conductance of CO2,

219 Only AQPs exhibited significant osmotic water permeability (P(f)).

220 We found high transepithelial water permeability (P(f), 54 +/- 5 microm/s) in airway e

221 d (ABA) or by high humidity, and the osmotic water permeability (P(os)) of protoplasts.

222 (Pf = 19 x 10(-3) cm/s) of the total osmotic water permeability pathway, and lipid permeation apparen

223 1.5 x 10(-3) cm/s) of the total diffusional water permeability pathway, and lipid permeation apparen

224 Diffusive water permeability (Pd) was 0.0024 +/- 0.0002 cm/s, and

225 ergy (Ea), mercurial inhibition, diffusional water permeability (Pd), and AQP expression.

226 ypertonic solution decreased the diffusional water permeability (Pdw) of the corneal epithelium.

227 f stopped-flow experiments yielded a unitary water permeability pf of (6.9 +/- 0.6) x 10(-13) cm(3)s(

228 rodissected plasma membranes, single channel water permeabilities (pf, referenced to the AQP1 pf of 6

229 eoliposomes, AqpZ displays very high osmotic water permeability (pf > or = 10 x 10(-14) cm3 s-1 subun

230 dose-dependent increases in osmotic membrane water permeability (Pf) (e.g. increased by 60% with 10(-

231 a, contributes to the modulation of membrane water permeability (Pf) and pH aquaporin regulation.

232 Osmotic water permeability (Pf) and urea permeability were measu

233 ression is associated with increased osmotic water permeability (Pf) between the airspace and capilla

234 Now, we test whether decreased osmotic water permeability (Pf) contributes to the concentrating

235 trategies to measure plasma membrane osmotic water permeability (Pf) in epithelial cells has been mot

236 Transepithelial osmotic water permeability (Pf) in isolated microperfused S2 seg

237 Osmotic water permeability (Pf) in plasma membrane vesicles from

238 se of this study was to test whether osmotic water permeability (Pf) in type I alveolar epithelial ce

239 Osmotic water permeability (Pf) in Xenopus oocytes injected with

240 Antidiuretic hormone modulates the water permeability (Pf) of epithelial cells in the rat k

241 ABA induced a 2-fold increase in osmotic water permeability (Pf) of guard cell protoplasts and an

242 Osmotic water permeability (Pf) of SM was high, with a value of

243 Quantitative measurement of single channel water permeability (pf) using epitope-tagged rat UTs gav

244 glucose transport was decreased, and osmotic water permeability (Pf) was increased.

245 Osmotic water permeability (Pf) was measured by change in light

246 Osmotic water permeability (Pf) was measured in double-perfused

247 Airspace-capillary osmotic water permeability (Pf) was measured in isolated perfuse

248 In this study, transepithelial osmotic water permeability (Pf) was measured in isolated perfuse

249 n Xenopus oocytes for measurement of osmotic water permeability (Pf), [3H]glycerol uptake, and protei

250 For measurement of osmotic water permeability (Pf), the airway lumen was perfused w

251 tes to determine the coefficients of osmotic water permeability (Pf).

252 gave a mercury-sensitive increase in osmotic water permeability (Pf).

253 nd type II alveolar epithelial cells to lung water permeability, Pf was measured by stopped-flow ligh

254 ther increase in the PLR reduced the osmotic water permeability probably due to the occurrence of def

255 O) of 1000-2000 Da but also have a high pure water permeability (PWP) of 82.5-117.6 L m(-2) h(-1) bar

256 Here, we address the hypothesis that this water permeability response is mediated in part through

257 during both the onset and maintenance of the water permeability response to vasopressin.

258 that the decrease in AVP-stimulated osmotic water permeability results, at least in part, in the dec

259 syntrophin, which manifest reduced astrocyte water permeability secondary to defective AQP4 plasma me

260 In addition to high water permeability, SM vesicles also show high mercury-s

261 carefully design a PRO membrane with a large water permeability, small B value, and reasonably small

262 hAQP1 water channels can transit from a high-water-permeability state to a closed state.

263 nt calmodulin inhibitors each increased AQP0 water permeability, suggesting that Ca(2+) may act throu

264 AQP1 protein and had 7-fold greater osmotic water permeability than did erythrocytes from null mice.

265 Previously, the only known blockers of water permeability through aquaporin-1 (AQP1) water chan

266 onstitution of AfAQP significantly increased water permeability through planar lipid bilayers, P(M,H(

267 Water permeability through single-file channels is affec

268 our variant AQP4 channels reduced normalized water permeability to between 26 and 48% of the referenc

269 cient and useful means to impart facilitated water permeability to cells lacking such a pathway.

270 AQP1-deficient cells, which increased their water permeability to that of wild-type cells, corrected

271 t constitutive surface expression levels and water permeability to wild-type AQP4.

272 oocytes exhibit significantly higher osmotic water permeability under basal conditions.

273 glycerol permeability more than 100-fold and water permeability up to 10-fold compared with control l

274 Occasionally an open state, with diffusive water permeability very close to that of WT AqpZ, was ob

275 Osmotic water permeability was >4-fold reduced in -/- than in +/

276 Erythrocyte water permeability was 7-fold reduced by AQP1 deletion b

277 system, and the effect of truncation on AQP0 water permeability was assessed in an oocyte osmotic swe

278 Although increased water permeability was confirmed in the Xenopus oocyte e

279 The reduction of water permeability was dose-dependent for tested concent

280 Osmotic water permeability was immeasurably small.

281 Osmotic water permeability was measured by stopped-flow light sc

282 Water permeability was measured for eight GOALs characte

283 Osmotic water permeability was measured in calcein-stained epith

284 passage through an aqueous channel, osmotic water permeability was measured in Xenopus oocytes expre

285 not increased above background, and osmotic water permeability was only minimally elevated.

286 embrane vesicles from knockout mice, osmotic water permeability was reduced 8-fold compared with vesi

287 oocytes for 15 min with 100 microM TEA, AQP1 water permeability was reduced by 20 to 40%, a degree of

288 rginine vasopressin (AVP)-stimulated osmotic water permeability was significantly reduced in rats fed

289 ion, membrane blebbing dynamics, and osmotic water permeability were assayed.

290 Changes in water permeability were associated with the overexpressi

291 ons after residues 234, 238, and 243 on AQP0 water permeability were examined.

292 The measurements of water permeability were found to scale exponentially wit

293 at specifically reduces AVP-elicited osmotic water permeability when luminal calcium rises.

294 ults in proteinuria and increased glomerular water permeability, which are both rescued by over-expre

295 elled under hyposmotic conditions indicating water permeability, which was abolished after preincubat

296 Specific regulation of membrane water permeability will likely prove important to unders

297 s, could explain the observed differences in water permeability with changes in bilayer thickness.

298 the per-channel increment in proteoliposome water permeability with the aid of stopped-flow experime

299 ave physiological consequences, because AQP4 water permeability would be reduced by its sequestration

300 ess aquaporins and thus display low inherent water permeability, yet SD rapidly induces focal swellin