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1 extracts from S. cerevisiae, N. crassa, and wheat germ.
2 f the dorsal horn, using nerve injections of wheat germ agglutin-horseradish peroxidase for transgang
3 monoclonal antibodies 1218 (anti-alpha) and wheat germ agglutinin (anti-beta), the H+, K(+)-ATPase i
4 binding peanut agglutinin (cone matrix) and wheat germ agglutinin (rod/cone matrix), was defined by
5 t distance between binding sites is ca. 9 A, wheat germ agglutinin (WGA) (shortest distance between b
7 PHA-L) or an adeno-associated virus encoding wheat germ agglutinin (WGA) and by immunoelectron micros
13 ytic components accessible to HRP-conjugated wheat germ agglutinin (WGA) disrupted delivery of HA but
14 rum against purified cyst walls (PCWs) or to wheat germ agglutinin (WGA) inhibited excystation by > 9
18 ally targeted the transneuronal tract tracer wheat germ agglutinin (WGA) to nonpeptidergic nociceptiv
19 of the binding of concanavalin A (ConA) and wheat germ agglutinin (WGA) to their target monosacchari
20 broad antibody-like reagent against mycosis, wheat germ agglutinin (WGA) was linked to the effector F
21 rgeted expression of a transneuronal tracer, wheat germ agglutinin (WGA), in the 5HT neurons so as to
22 which expression of a transneuronal tracer, wheat germ agglutinin (WGA), is induced in primary senso
23 ermined using a Texas Red-conjugated lectin, wheat germ agglutinin (WGA), which binds SA residues.
24 ity HPLC column charged by Co2+, followed by wheat germ agglutinin (WGA)-affinity HPLC or size-exclus
35 port of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP) with biotinylated dextra
38 ity purification steps on Ni-NTA agarose and wheat germ agglutinin agarose provided substantial enric
39 text of both the sugar and the lectin (here, wheat germ agglutinin and a single hevein domain) and ca
40 s demonstrated by blotting with succinylated wheat germ agglutinin and by probing with bovine milk be
41 ovascular endothelial cells was inhibited by wheat germ agglutinin and chitooligomers prepared from t
42 ents to NMC by using retrograde transport of wheat germ agglutinin and horseradish peroxidase (WGA-HR
44 otting and were purified from bovine BMEC by wheat germ agglutinin and Maackia amurensis lectin (spec
46 clear assembly by liposomes was inhibited by wheat germ agglutinin and thus required active nuclear t
47 s was temperature dependent and sensitive to wheat germ agglutinin and was blocked by a 20-fold exces
48 it was a glycoprotein in that it adhered to wheat germ agglutinin and was eluted with N-acetyl gluco
51 domain is the site of glycosylation based on wheat germ agglutinin binding activity of polypeptides p
53 ociation with the alpha2delta subunit during wheat germ agglutinin chromatography, repeat III by itse
57 grade transport after intranasal infusion of wheat germ agglutinin conjugated horseradish peroxidase
58 injections of biotinylated dextran amine and wheat germ agglutinin conjugated horseradish peroxidase
59 tion neurons (PN), retrogradely labeled with wheat germ agglutinin conjugated horseradish peroxidase
60 C57BL/6J) mice using the anterograde tracer, wheat germ agglutinin conjugated horseradish peroxidase.
61 injections of biotinylated dextran amine and wheat germ agglutinin conjugated to horseradish peroxida
62 e NRA-lumbosacral projection with the use of wheat germ agglutinin conjugated to horseradish peroxida
64 body was investigated in adult cats by using wheat germ agglutinin conjugated to horseradish peroxida
65 dult cats using biotinylated dextran amines, wheat germ agglutinin conjugated to horseradish peroxida
66 projections were labeled with injections of wheat germ agglutinin conjugated to horseradish peroxida
67 rs biocytin, biotinylated dextran amine, and wheat germ agglutinin conjugated to horseradish peroxida
68 of several different fluorescent tracers and wheat germ agglutinin conjugated to horseradish peroxida
73 extran, fluorescent dextran, 3H-leucine, and wheat germ agglutinin conjugated to horseradish peroxida
74 d green fluorescent latex microspheres or of wheat germ agglutinin conjugated to horseradish peroxida
75 ction was further studied with injections of wheat germ agglutinin conjugated to horseradish peroxida
76 ted leucine, biotinylated dextran amine, and wheat germ agglutinin conjugated to horseradish peroxida
77 d spinal trigeminal nuclei were labeled with wheat germ agglutinin conjugated to horseradish peroxida
79 orescent latex microspheres or injections of wheat germ agglutinin conjugated to horseradish peroxida
82 in subunit beta (CTB), Fluoro-ruby (FR), and wheat germ agglutinin conjugated with horseradish peroxi
83 Injections of the retrograde pathway tracer wheat germ agglutinin conjugated with horseradish peroxi
84 modulin-dependent transport was inhibited by wheat germ agglutinin consistent with transport proceedi
85 ng nuclear pore formation with microinjected wheat germ agglutinin does not inhibit the nuclear local
86 Ac-containing glycoproteins using the lectin wheat germ agglutinin dramatically enriches for CTD110.6
91 cate midbrain neurons projecting to the NRA, wheat germ agglutinin horseradish peroxidase (WGA-HRP) w
92 ic mice expressing the trans-synaptic tracer wheat germ agglutinin in LepRb neurons reveal the innerv
94 ty with Griffonia simplicifolia lectin I and wheat germ agglutinin induced serum IgM Abs in mice that
98 b2 to endothelial ELK receptors recovered by wheat germ agglutinin lectin and immunoprecipitation.
100 ulfate-proteoglycan antibody reactivity, and wheat germ agglutinin lectin staining of the metanephros
101 ns like Griffonia simplicifolia lectin I and wheat germ agglutinin mediate the apoptosis of tumor cel
102 irE2-ssDNA complex import in the presence of wheat germ agglutinin or a nonhydrolyzable GTP analog, b
103 he import was ATP-dependent and inhibited by wheat germ agglutinin or by an antibody against p97, a c
104 horseradish peroxidase conjugated to either wheat germ agglutinin or cholera toxin subunit B reveale
105 lar AF-ALN colocalized with dextran (but not wheat germ agglutinin or transferrin), and uptake of AF-
108 nfocal microscopy with fluorescently labeled wheat germ agglutinin showed a paucity of PNAG in S. lug
109 overed that the carbohydrate-binding protein wheat germ agglutinin specifically stains colonies and t
113 interneurons and the transganglionic tracer wheat germ agglutinin which, after sciatic nerve injecti
114 luorescein, Lucifer Yellow or FluoroRuby, or wheat germ agglutinin) into discrete VA/VL, MD, and fron
115 pergillus oryzae lectin [AOL] and binding of wheat germ agglutinin) were assessed in paraffin-embedde
116 promotes the expression of the tracer, WGA (wheat germ agglutinin), and used these in combination wi
118 not oriented as in the crystal structure of wheat germ agglutinin, a highly homologous protein ( app
119 ia, peanut agglutinin, Ricinis communis, and wheat germ agglutinin, and by ATPase and ADPase enzymati
120 rs bind differentially to concanavalin A and wheat germ agglutinin, and lectin-affinity chromatograph
121 eiraea simplicifolia lectin II, succinylated wheat germ agglutinin, and peanut agglutinin were signif
122 ty with the chitin-binding domain of hevein, wheat germ agglutinin, and several class I chitinases.
123 nergy-dependent, was inhibited by the lectin wheat germ agglutinin, and showed an absolute requiremen
124 be inhibited by pretreating the nuclei with wheat germ agglutinin, anti-NPC antibodies, or antibodie
125 nd to immobilized Concanavalin A (Con A) and wheat germ agglutinin, as well as to immobilized recombi
126 rt of a genetically expressed lectin tracer, wheat germ agglutinin, in Na(V)1.8-expressing nociceptor
128 f free virus and that uptake was enhanced by wheat germ agglutinin, strongly suggesting that the enve
129 alent cation independent but is inhibited by wheat germ agglutinin, suggesting that the major recepto
130 nuclear uptake of GFP-ERK2 was inhibited by wheat germ agglutinin, which blocks nuclear entry by bin
131 und that AMPK was recognized by succinylated wheat germ agglutinin, which specifically binds O-GlcNAc
132 tracted from brain membranes associated with wheat germ agglutinin-affinity columns, was [3H]galactos
134 trogradely labeled after focal injections of wheat germ agglutinin-apo (inactivated) horseradish pero
136 emonstration that chitotriose-bound OmpA and wheat germ agglutinin-bound brain microvascular endothel
137 the VTA by combining retrograde transport of wheat germ agglutinin-bound gold after injections into t
140 ement, we injected the bidirectional tracer, wheat germ agglutinin-conjugated horseradish peroxidase
141 retrogradely labeled following injections of wheat germ agglutinin-conjugated horseradish peroxidase
142 old: ventral Vi/Vc, or MDH) or peripherally (wheat germ agglutinin-conjugated horseradish peroxidase
145 t nodose ganglion with 3 microl of either 4% wheat germ agglutinin-horseradish peroxidase (to label s
146 e identified regions was determined by using wheat germ agglutinin-horseradish peroxidase (WGA-HRP) a
149 e labeled either by anterograde transport of wheat germ agglutinin-horseradish peroxidase (WGA-HRP) f
150 injected 18 weeks later with 3 microl of 4% wheat germ agglutinin-horseradish peroxidase (WGA-HRP) i
151 placed injections of fluorescent tracers and wheat germ agglutinin-horseradish peroxidase (WGA-HRP) i
152 bbit maxillary sinus were localized by using wheat germ agglutinin-horseradish peroxidase (WGA-HRP) o
153 neuronal transport of intraocularly injected wheat germ agglutinin-horseradish peroxidase (WGA-HRP) o
156 thesis, we have analyzed the distribution of wheat germ agglutinin-horseradish peroxidase (WGA-HRP)-l
157 ranch, and were injected with 3 microl of 8% wheat germ agglutinin-horseradish peroxidase in the left
158 adult owl monkeys by means of injections of wheat germ agglutinin-horseradish peroxidase into the ap
161 were labeled via iontophoretic injections of wheat germ agglutinin-horseradish peroxidase into the LG
162 gastrointestinal tract, the authors injected wheat germ agglutinin-horseradish peroxidase into the no
163 sensory areas by making small injections of wheat germ agglutinin-horseradish peroxidase into the sp
164 imals received nodose ganglion injections of wheat germ agglutinin-horseradish peroxidase or dextran-
165 We, therefore, placed discrete injections of wheat germ agglutinin-horseradish peroxidase or fluoresc
166 ections of either of two retrograde tracers, wheat germ agglutinin-horseradish peroxidase or fluoresc
168 animals were randomly assigned to afferent (wheat germ agglutinin-horseradish peroxidase) or efferen
169 ere labeled by nodose ganglion injections of wheat germ agglutinin-horseradish peroxidase, and a stan
170 stricted injections of fluorochrome tracers, wheat germ agglutinin-horseradish peroxidase, or biotiny
175 zyme with neuraminidase prevented binding to wheat germ agglutinin-Sepharose, indicating the presence
189 identified by combining a retrograde tracer (wheat-germ agglutinin apo-horseradish peroxidase colloid
190 ran linked to fluorescein or, alternatively, wheat-germ agglutinin conjugated to an Alexa fluor dye.
191 onodelphis domestica were investigated using wheat-germ agglutinin conjugated to horseradish peroxida
192 a glycan variant on MUC5AC using the lectin wheat-germ agglutinin discriminated mucin-producing cyst
193 ed with horseradish peroxidase conjugated to wheat-germ agglutinin were in asymmetric synaptic contac
195 e conducted tract tracing experiments (using wheat-germ agglutinin-horseradish peroxidase (WGA-HRP),
197 been depleted of endogenous hsp70, purified wheat germ and mouse hsp70's are equally active in promo
198 wo other plant viruses as well as tRNAs from wheat germ and yeast were similarly active in the BMV vi
199 ately 10 mg of the pure protein from 4 kg of wheat-germ), and improved characteristics of stability a
200 operties of this enzyme, we used a cell-free wheat germ-based expression system in which mRNA encodin
202 nts to identify the structural properties of wheat germ calmodulin (CaM) bound to either the plasma m
203 Oxidation of either Met(145) or Met(146) in wheat germ calmodulin (CaM) to methionine sulfoxide prev
204 ative modification of methionine residues in wheat germ calmodulin (CaM), and prevent activation of t
206 The concentrations of [F138]calmodulin and wheat germ calmodulin required for half-maximal activati
207 tant containing tyrosine at position 99, and wheat germ calmodulin which has tyrosine at position 139
209 itions of oxidative stress, we have examined wheat germ CaM for the presence of highly reactive sites
210 trotyrosine139 in calcium binding loop IV on wheat germ CaM indicate that the average spatial separat
214 levels in animal myocytes, E. coli, and the wheat germ cell-free system than Mbs from terrestrial ma
215 falciparum topoisomerase II (PfTopoII) in a wheat germ cell-free transcription-translation system.
216 es in nuclear extracts prepared from pea and wheat germ, consistent with the hypothesis that the Arab
223 degree of homology to the p82 subunit of the wheat germ eukaryotic translation initiation factor eIF-
224 ction between VPg of turnip mosaic virus and wheat germ eukaryotic translation initiation factors eIF
227 cate in oat protoplasts, indicating that the wheat germ extract accurately reflected control of BYDV
228 s strong cap-independent translation in both wheat germ extract and oat protoplasts through a novel,
229 xhibits translational inhibition in both the wheat germ extract and rabbit reticulocyte lysate system
232 unidentified proteins or small molecules in wheat germ extract prevented eIF4F binding to mutant BTE
233 d robust blocks to both Escherichia coli and wheat germ extract translation systems, whereas N2-methy
234 functional shifty site for frameshifting in wheat germ extract, while the stop codon was not require
236 , we developed an in vitro transcription and wheat germ extract-based translation assay to examine qu
239 , and the resulting mRNAs were translated in wheat germ extracts and radiolabeled with either [35S]me
240 for this programmed readthrough in vitro in wheat germ extracts and reticulocyte lysates and in vivo
243 RegA69-His6 synthesized in E. coli S30 or wheat germ extracts displayed RNA-binding properties sim
249 identify their enzymatic properties using a wheat germ in vitro translation (IVT, also known as cell
250 d alfalfa mosaic virus (AMV) 4, were used in wheat germ in vitro translation assays to measure their
253 or aspartate transcarbamoylase (ATCase) from wheat-germ is reported, with an eightfold increase in sc
256 KBP to plant hsp90 is prevented by adding to wheat germ lysate a purified fragment containing the TPR
258 the JJ3 antibody to protein A-Sepharose, to wheat germ lysate and allow ATP-dependent formation of a
259 tent inhibitor of PAP depurination of RNA in wheat germ lysate and competes with structured RNA deriv
260 The hsp90/hsp70-based chaperone system of wheat germ lysate assembles complexes between mouse GR a
262 tion of known Sec incorporation factors in a wheat germ lysate does not permit multiple Sec incorpora
263 ibits the translation of multiple mRNAs in a wheat germ lysate, suggesting that Prbp acts to repress
264 o contain endogenous PKR but was not seen in wheat germ lysate, which is not responsive to a known ac
267 nding of high risk HPV E6 proteins to PKN in wheat-germ lysate in vitro and in 293T cells in vivo.
268 ls) and insect (Sf9) cells and also a plant (wheat germ) lysate fold the immunopurified glucocorticoi
269 ome mosaic virus RNA in vitro translation in wheat germ lysates by the addition of double-stranded RN
270 o under physiological buffer conditions with wheat germ methionyl-tRNA synthetase, required mutation
271 ly marine invertebrates ( approximately 1%); wheat germ or bran ( approximately 1%); and spinach ( ap
277 ng the mechanism of helicase activity in the wheat germ protein synthesis system, we have utilized di
278 quantitatively assessed using higher plant (wheat germ) proteins: aminoacylation, EF-1alpha*GTP bind
279 an in vitro translation lysate derived from wheat germ repressed translation, which was subsequently
280 Interestingly, we found that mammalian and wheat germ ribosomes differentially regulate the signal-
281 e discovered that when Pgp was translated by wheat germ ribosomes in vitro, TM3 could not reinitiate
282 ents of Pgp and that rabbit reticulocyte and wheat germ ribosomes may use different mechanisms to con
283 ncharged tRNA and initiator Met-tRNAMet from wheat germ, RNAs that are normally excluded from the rib
284 n of mRNA produced from this clone in both a wheat germ system and Xenopus oocytes showed expression
285 nction was analyzed in Neurospora crassa and wheat germ translation extracts using the transfer of na
288 abeled ppalphaF6H was affinity purified from wheat germ translation reactions (or Escherichia coli) t
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