ライフサイエンスコーパス: whole-cell

1 racellular calcium handling phenomena at the whole-cell.

2 rmanent magnet using enzyme solutions and in whole cells.

3 and microtubules and actin stress fibers in whole cells.

4 based media or assayed by fructose uptake in whole cells.

5 actomycin (TLM) analogues that show improved whole cell activity against bacterial strains including

6 ased on the use of Micrococcus lysodeikticus whole cells adsorbed on graphene oxide (GO)-coated Surfa

7 protein, miRNA, whole cell and biomarkers generated by metabolic shift i

8 e cholera vaccine (killed Vibrio cholerae O1 whole cells and recombinant cholera toxin B subunit) aff

9 Fluorescence lifetime microscopy of whole cells and ultrafast transient absorption spectrosc

10 ent stored sera for varicella antibody using whole-cell and glycoprotein enzyme-linked immunosorbent

11 use TMEM16A expressed in HEK-293 cells using whole-cell and inside-out patch-clamp recordings.

12 y of DG granule cells (GCs) while performing whole-cell and juxtacellular recordings of CA3 neurons i

13 Here, we combine in vivo whole-cell and multisite extracellular recordings to cha

14 cterization by means of electrophysiological whole-cell and single-channel recordings as well as Ca(2

15 Comparisons of whole-cell and single-channel SLO-2 currents in native n

16 ilutions from 1:100 to 1:102,400 using crude whole-cell antigens of the Karp, Kato, and Gilliam strai

17 ells are typically interrogated using single whole cell approaches.

18 Whole cells are labelled via click chemistry and visuali

19 comparing expression data between nuclei and whole cells are lacking.

20 their reported activities against kinases in whole-cell assays.

21 area of selective recognition and capture of whole cells, based on natural receptors, as well as synt

22 dular Escherichia coli and Bacillus subtilis whole-cell-based biosensors which incorporate an interch

23 bacteria against novel compounds isolated by whole-cell-based high-throughput screening (HTS).

24 show that BKCa-CaV stoichiometry can affect whole-cell behavior substantially.

25 Here, we report the whole-cell bioconversion of citrate to itaconate with en

26 Using this whole-cell bioconversion system, we were able to catalyz

27 ve ion exchange, selective ion exchange, and whole-cell biological reduction) and emerging (catalysis

28 this study, we developed a novel dual-signal whole-cell biosensor for the detection of dopamine throu

29 A whole-cell biosensor utilizing a transcription factor (T

30 Whole cell biosensors have been seldom used in the pharm

31 ed for eukaryotic cells, and can be used for whole cell biosensors, or in real time with live animals

32 nts of a compound of interest are central to whole-cell biosensors design for medical and environment

33 In combination with whole-cell biotransformations, these stereocomplementary

34 ative measurements of in vivo activity using whole-cell biotransformations, where two Escherichia col

35 r work provides a simple formulation for the whole-cell BKCa current that respects local interactions

36 scale analysis, we derive a concise model of whole-cell BKCa currents, which can readily be analyzed

37 successfully measured via flow cytometry in whole cells but rarely in isolated mitochondria from lar

38 rotrusions enriched in zinc were detected on whole cells by scanning electron microscopy and imaging

39 Finally, wortmannin selectively reduced whole cell Ca(2+) currents in OT neurons while leaving V

40 vels affected AHPs, somatic [Ca(2+) ]i , and whole cell Ca(2+) currents.

41 We found significantly larger whole-cell Ca(2+) currents from diseased neurons that we

42 V1.2 channels and consequently decreased the whole-cell Ca(2+) influx via the CaV1.2 channels.

43 esponses have been observed as spikes of the whole-cell calcium concentration in numerous cell types

44 al ciliary cAMP that is fivefold higher than whole-cell cAMP.

45 Evolved in the context of whole-cell catalysts, the proteins were more active in t

46 Using whole-cell clamp methods, we characterized the temporal

47 A whole cell cryo electron tomogram contains structural in

48 subunit interfaces produced minor changes in whole cell current peak amplitude but altered current de

49 bunit stoichiometry or decreased GABA-evoked whole-cell current amplitudes, but with different levels

50 Tat (4-16 h) potentiated NMDA-evoked whole-cell current and increased the NMDAR:AMPAR ratio o

51 Guangxitoxin, a Kv2 blocker, inhibited the whole-cell current by approximately 50%, and enhanced 2-

52 Using whole-cell current clamp recording, we found that L2/3 p

53 Immunohistochemistry experiments and whole-cell current recordings in human embryonic kidney

54 Whole-cell current- and voltage-clamp recordings were ma

55 We conducted whole-cell current-clamp and single-unit recordings in N

56 Whole-cell current-clamp recordings demonstrated increas

57 located at beta+ subunit interfaces reduced whole cell currents by decreasing single channel open pr

58 Shear stress evoked a rapid increase in whole cell currents in oocytes expressing degenerin chan

59 tion and completely abolishes cAMP-activated whole cell currents.

60 AMPARs associated with Type I TARPs, evoked whole-cell currents in lamina II neurons, but such curre

61 Whole-cell currents in response to application of acetyl

62 3-isobutyl-1-methylxanthine (IBMX)-activated whole-cell currents in the presence of the corrector.

63 We found that UBP684 potentiated the whole-cell currents observed under perforated-patch cond

64 However, GABA-evoked whole-cell currents of DKO neurons and the GABAAR cell s

65 alter the functional up-regulation of CaV1.2 whole-cell currents.

66 vo and increased Wnt-activated PC2-dependent whole-cell currents.

67 ir size, and that size control acts over the whole cell cycle, rather than specifically in G1.

68 Experiments in whole cells demonstrated that these four residues partic

69 g enhanced detection sensitivity compared to whole-cell dissection by minimizing chemical interferenc

70 are artificially induced during conventional whole-cell dissociation procedures.

71 s, which are subsequently used as input to a whole-cell DPD model to predict the RBC shape and corres

72 (comparable to that of verapamil) toward the whole-cell drug efflux pump activity of mycobacteria, th

73 tional approach for de novo sequencing using whole cell E. coli lysate.

74 we present Patch-seq, a method that combines whole-cell electrophysiological patch-clamp recordings,

75 Whole-cell electrophysiological recordings detected a si

76 Whole-cell electrophysiological recordings revealed alte

77 Whole-cell electrophysiological recordings were used to

78 We used whole-cell electrophysiology and anatomical methods to a

79 To address this question, we used whole-cell electrophysiology and determined the intrinsi

80 ted, GFP+ neurons in these brain areas using whole-cell electrophysiology in brain slices.

81 Using whole-cell electrophysiology in juvenile-adolescent GAD6

82 Although whole-cell electrophysiology is the gold standard for fu

83 Here, we used whole-cell electrophysiology to measure CDI and computat

84 eterologously in Xenopus laevis oocytes, and whole-cell electrophysiology was used to monitor channel

85 With a validated Fezf2-Gfp reporter mouse, whole-cell electrophysiology with morphology reconstruct

86 1(ARH) neuronal activity using optogenetics, whole-cell electrophysiology, molecular pharmacology and

87 n by in utero electroporation of shRNAs with whole-cell electrophysiology.

88 Whole-cell ELISA was used to establish the surface stain

89 by the eukaryotic ribosome in cell-free and whole-cell environments and can be incorporated into sol

90 e have found that Def1 copurifies from yeast whole-cell extract with TFIIH, the largest general trans

91 ty of different mutations appears similar in whole-cell extracts from lymphocytes, and suggest that m

92 ical biosensors targeting small molecules or whole cells for use in point of care biosensing.

93 s asymmetric viruses, cellular organelles or whole cells from a series of tilted electron cryo-micros

94 Whole-cell FTIR analysis and comparative isotopologue pr

95 in a context specific manner to give rise to whole cell function.

96 The established SAR, using whole cell functional assays, lead to the full agonist 9

97 hips enables us to describe the emergence of whole cell functions from interacting SCPs.

98 iption of how SCPs together can give rise to whole cell functions.

99 networks for predictive modeling of emergent whole cell functions.

100 We developed and optimized a whole-cell Hg bioreporter capable of functioning under a

101 This whole cell impedance-based technology allows for the rea

102 Whole-cell imprinted polymers have the potential to be a

103 Whole-cell inclusion immunofluorescence serum antibody t

104 s question here using neural simulations and whole cell intracellular recordings in combination with

105 The effect of arsenic exposure on whole-cell intracellular microbial metabolism, however,

106 C ensure that the rate of RA biosynthesis in whole cells is largely independent of the concentration

107 lting peptide (TAT-C1aB) suppressed enhanced whole-cell K(+) currents produced by a mutated form of K

108 ction of KCNQ2 in the AIS and a reduction in whole-cell KCNQ currents.

109 a decrease in surface KV 1.5, a reduction in whole-cell KV 1.5 currents and a loss of functional KV 1

110 ease in cell surface KV 1.5 channels reduces whole-cell KV 1.5 currents and attenuates KV 1.5 functio

111 Angiotensin II reduced both whole-cell KV currents and currents inhibited by Psora-4

112 Here we have employed whole cell labelling with azidomyristic acid and click c

113 y mass spectrometry is widely applied at the whole-cell level, it is not routinely possible to measur

114 MS/MS analysis (HILIC-RPLC) of S. cerevisiae whole cell lysate has been used to acquire retention inf

115 this study, a microparticulate vaccine using whole cell lysate of a murine ovarian cancer cell line,

116 C-MS/MS analysis (SCX-RPLC) of S. cerevisiae whole cell lysate was used to generate a retention datas

117 ll-like receptor ligands and M. tuberculosis whole-cell lysate, increased M. tuberculosis replication

118 isolation of O-glycans from glycoproteins in whole cell lysates for mass spectrometric analysis.

119 Chemical treatments of M. tuberculosis whole-cell lysates (MtbWL) ruled out protein, nucleic ac

120 asured in the lysosomal fraction, but not in whole-cell lysates.

121 Whole-cell, macropatch, and single-channel electrophysio

122 Previous measurements of whole-cell macroscopic currents showed that alpha4 and b

123 ndocytosis at rat calyx of Held terminals by whole-cell membrane capacitance measurements.

124 his is associated with a gradual increase in whole-cell membrane capacitance.

125 Whole-cell membrane potential recordings and silicon pro

126 o as the engineered enzyme assembly improved whole-cell methanol consumption rate by ninefold.

127 ditional viable count techniques; the use of whole cell microbial biosensors potentially provides an

128 Whole cell microprobe SR-XRF identified endogenous Cu in

129 r BKCa description into previously published whole-cell models, and perform simulations of electrical

130 heir eventual integration into comprehensive whole-cell models.

131 Whole-cell mount has the simplest sample preparation but

132 provided, including Tokuyasu cryosectioning, whole-cell mount, cell unroofing and platinum replicatio

133 plasma membrane but is more challenging than whole-cell mount.

134 strate this system's capabilities to isolate whole cells, mRNA, and DNA, demonstrating up to a 28-fol

135 We employ a two-component whole-cell multiscale model to quantify the biomechanica

136 Whole cell NMR analysis also revealed that S. aureus, in

137 action for amphomycin and MX-2401 in intact whole cells of Staphylococcus aureus by measuring the ch

138 Killed whole-cell oral cholera vaccines (kOCVs) are becoming a

139 Whole-cell outward currents were maximal at +50 mV and d

140 inuous-flow approach based on an immobilized whole cells-packed bed reactor.

141 We performed whole cell patch clamp recordings of hippocampal neurons

142 Using whole cell patch recordings, sympathetic preganglionic n

143 Using optogenetics, cell-specific ablation, whole cell patch-clamp and immuno-electron microscopy, w

144 rophysiological studies were conducted using whole cell patch-clamp to explore biophysical properties

145 on of four selected missense mutations using whole cell patch-clamping in tsA201 cells-together with

146 glucose sensing by Sf1 neurons, we performed whole-cell patch clamp analysis of brain slices from con

147 f dendritic spines in the CeA and, moreover, whole-cell patch clamp analysis of the basal amygdala to

148 Here, using whole-cell patch clamp and Western blotting analysis, we

149 Here, we used whole-cell patch clamp electrophysiology and neuronal re

150 t traffics to the plasma membrane, but using whole-cell patch clamp electrophysiology we observed tha

151 We used mutagenesis and whole-cell patch clamp experiments on TRPV3 channels end

152 -G2 exhibited similar channel properties in whole-cell patch clamp experiments.

153 A recent study using whole-cell patch clamp recording of MNs in acute spinal

154 We used whole-cell patch clamp recording to investigate the effe

155 ons in mouse neocortical brain slices during whole-cell patch clamp recording.

156 We combined whole-cell patch clamp recordings (n = 440) of NMDAR-med

157 ssion at the endbulb of Held was assessed by whole-cell patch clamp recordings from auditory neurons

158 ssion at the endbulb of Held was assessed by whole-cell patch clamp recordings from auditory neurons

159 ically obtaining high-yield and high-quality whole-cell patch clamp recordings in vivo.

160 nderwent one of the following procedures: 1) whole-cell patch clamp recordings to characterize AMPAR

161 Whole-cell patch clamp showed that CASK-silencing increa

162 embrane current in atrial myocytes using the whole-cell patch clamp technique, combined with pressuri

163 ythmias (VA) were probed by optical mapping, whole-cell patch clamp to measure action potential durat

164 or genetic approaches that we confirmed with whole-cell patch clamp to substantially reduce Ih curren

165 Whole-cell patch clamp was used to assess silent synapse

166 tant hEAAT1 in mammalian cells and performed whole-cell patch clamp, fast substrate application, and

167 Using whole-cell patch clamp, we have demonstrated that NTnC d

168 y isolated rabbit ventricular myocytes using whole-cell patch clamp.

169 flow system, and the current was measured by whole-cell patch clamp.

170 a functional experimental rig for automated whole-cell patch clamping can be set up in 1 week.

171 Whole-cell patch clamping in vivo is an important neuros

172 ectroscopy and APOL1-dependent currents with whole-cell patch clamping.

173 hin from D1R-SPNs in brain slice while using whole-cell patch recording to measure changes in the syn

174 iatal projection neurons when measured using whole-cell patch recording.

175 electrophysiological gradients, we performed whole-cell patch recordings along the dorsal-ventral axi

176 stigate these mechanisms we combined in vivo whole-cell patch recordings from midbrain neurons, extra

177 Furthermore, in dual whole-cell patch recordings in neonatal CA1, MGE interne

178 racellular fillings of neurons in vitro, and whole-cell patch recordings to characterize the connecti

179 ation of DG granule cells while recording in whole-cell patch-clamp and juxtacellular configuration f

180 recorded in brain slices from SNL rats using whole-cell patch-clamp conditions.

181 Whole-cell patch-clamp electrophysiological recording is

182 anol (CIE) exposure to induce dependence and whole-cell patch-clamp electrophysiology was used to exa

183 Using whole-cell patch-clamp electrophysiology, activation of

184 Whole-cell patch-clamp measurements on root cell protopl

185 onto stellate cells in the cerebellum using whole-cell patch-clamp recording and photolytic uncaging

186 Here, in vivo whole-cell patch-clamp recording of excitatory neurons r

187 Applying whole-cell patch-clamp recording of HEK cells, we found

188 brain slices were harvested and prepared for whole-cell patch-clamp recording, and in treated rats, t

189 18.5 hypoglossal MNs from brain slices using whole-cell patch-clamp recording, followed by dye-fillin

190 veloped Patch-seq, a technique that combines whole-cell patch-clamp recording, immunohistochemistry,

191 To examine this, we used a combination of whole-cell patch-clamp recordings and optogenetics to de

192 , using pathway-specific retrograde tracing, whole-cell patch-clamp recordings and post hoc cell type

193 In whole-cell patch-clamp recordings from acute hippocampal

194 Utilizing whole-cell patch-clamp recordings from morphologically i

195 Using whole-cell patch-clamp recordings from spinal motoneuron

196 Whole-cell patch-clamp recordings in brain slices reveal

197 ippocampal stimulation in vivo and conducted whole-cell patch-clamp recordings in brain slices to rev

198 th muscle (aSM)-like activity patterns using whole-cell patch-clamp recordings in HEK293 cells (Cav1.

199 Using whole-cell patch-clamp recordings in posthearing mice, w

200 Whole-cell patch-clamp recordings in rat DRG neurons rev

201 tic suppression of HP inputs onto MSNs using whole-cell patch-clamp recordings in slices from adult r

202 Whole-cell patch-clamp recordings in the spinal cord sli

203 erves the intact cell-medium interface using whole-cell patch-clamp recordings in vivo and in vitro.

204 We used whole-cell patch-clamp recordings of over 460 neurons to

205 Whole-cell patch-clamp recordings revealed increased exc

206 viral tracer in male lean and db/db mice and whole-cell patch-clamp recordings were conducted.

207 Whole-cell patch-clamp recordings were made from layer V

208 We used in vitro whole-cell patch-clamp recordings with laser-scanning ph

209 Using whole-cell patch-clamp recordings, we observed a K(+) co

210 Here, using in vitro whole-cell patch-clamp recordings, we show that 5-HT hyp

211 Examination of neuronal activity by whole-cell patch-clamp shows elevated levels of glutamat

212 al characteristics of these neurons by using whole-cell patch-clamp technique in vitro Our results sh

213 h, as described following diphtheria-tetanus-whole cell pertussis (DTP) vaccination.

214 d only received 1 dose of Diphtheria Tetanus whole cell Pertussis (DTwP).

215 ates are based on studies in unvaccinated or whole-cell pertussis-vaccinated children.

216 ccine (which covers diphtheria, tetanus, and whole-cell pertussis; hepatitis B; and Haemophilus influ

217 he patch-clamp technique was used to measure whole-cell plasma membrane capacitance, and in fixed cel

218 f individual cells and flow cytometry of the whole cell population.

219 l AR activity can mirror the response in the whole cell population.

220 dynamics, including isolated transients and whole-cell propagating waves.

221 ach to quantify 1300 cell surface and 7200 whole cell proteins, we demonstrate that the US12 family

222 tutzeri DSM4166 was performed in unison with whole-cell proteomic analysis of BIRD-1 grown under phos

223 e used multiplex tandem mass tag (TMT)-based whole cell proteomics to perform a comprehensive time co

224 Whole cell recording showed that blue light (470 nm) eli

225 By whole cell recording, these fusions are fully functional

226 urons that synthesize POMC, as determined by whole cell recording.

227 Here we use in vivo whole-cell recording and two-photon Ca(2+) imaging in aw

228 uced into neocortical pyramidal cells during whole-cell recording and, using a combination of experim

229 Using homologous binding and whole-cell recording assays, we found that an RNA aptame

230 t silencing of STN neurons as measured using whole-cell recording ex vivo.

231 Here, by whole-cell recording of synaptic responses in MeCP2 muta

232 Whole-cell recording revealed that hippocampal pyramidal

233 microscopy with glutamate photo-uncaging and whole-cell recording to examine synaptic strength at ind

234 er-pulse photolysis technique, combined with whole-cell recording, we measured the rate of channel op

235 Using whole cell recordings in coronal hypothalamic slices fro

236 Whole cell recordings revealed that the isoflurane-activ

237 In vivo whole cell recordings suggest that feedforward inhibitio

238 Whole cell recordings were used to examine effects of vo

239 In the present study, we combined whole cell recordings with laser scanning photostimulati

240 Here, we used paired whole-cell recordings and optogenetic approaches in mous

241 Third, in vitro whole-cell recordings demonstrate that 50% (18/36) of OV

242 In vitro whole-cell recordings demonstrate the majority of OVLT n

243 Using whole-cell recordings from CA1 pyramidal cells and field

244 Here, we made whole-cell recordings from calyceal terminals in newborn

245 During whole-cell recordings from hippocampal and neocortical s

246 cerebellar activity during learning, we made whole-cell recordings from larval zebrafish Purkinje cel

247 By performing two-photon guided whole-cell recordings from layer 2/3 excitatory and inhi

248 the dendrites, we made dendritic and somatic whole-cell recordings from MSO principal neurons in brai

249 ribute to this imbalance, we obtained paired whole-cell recordings from striatal direct- and indirect

250 Using triple and dual whole-cell recordings from synaptically connected human

251 Using dual whole-cell recordings from the IO of young adult rhesus

252 Using whole-cell recordings in brain slices, we found that dyn

253 Whole-cell recordings in L2/3 of awake mice revealed tha

254 smission in the rat PL were determined using whole-cell recordings in layer V pyramidal neurons.

255 ing mice and concomitant field potential and whole-cell recordings in slice preparations we found tha

256 In vivo whole-cell recordings in the hypothalamus confirmed near

257 Whole-cell recordings in vitro showed that AIA enhanced

258 to glycinergic afferents in the ICC and made whole-cell recordings in vitro while exciting glycinergi

259 synaptic integration across species, we made whole-cell recordings in vivo from the murine LGN during

260 Omitting VX-809 during whole-cell recordings led to a spontaneous decline of th

261 Using whole-cell recordings of layer V pyramidal neurons in an

262 In vivo whole-cell recordings reveal that nearly every action po

263 Cell-attached and whole-cell recordings revealed that excitatory neuron fi

264 Whole-cell recordings revealed that striatal inputs to t

265 Whole-cell recordings showed that excitatory inputs were

266 We performed dual dendritic and somatic whole-cell recordings to measure spontaneous EPSPs using

267 Whole-cell recordings were conducted in male offspring b

268 In whole-cell recordings, G2019S SPNs exhibited a fourfold

269 Using in vivo whole-cell recordings, we show that the timing of an LN'

270 gonists, we performed simultaneous field and whole-cell recordings.

271 Whole cell responses arise from coordinated interactions

272 Since whole cell responses most often arise from the coordinat

273 the sarcoplasmic reticulum structure at the whole-cell scale, by reducing its full 3-D structure to

274 Phenotypic whole-cell screening in erythrocytic cocultures of Plasm

275 ld was previously identified by target-based whole-cell screening.

276 The MTTT protocols included (1) a whole-cell sonicate (WCS) EIA followed by a C6 EIA; (2)

277 A) has been approved to replace the standard whole-cell sonicate EIA as a first-tier test for the dia

278 ot if the C6 EIA result was positive but the whole-cell sonicate EIA result was negative.

279 steps mixed responses caused by uncontrolled whole-cell swamping with reactive signals.

280 rize potential GLUT5 ligands, we developed a whole-cell system based on a yeast strain deficient in f

281 alysts, the proteins were more active in the whole-cell system than in purified forms.

282 of biocatalysts (including both enzymes and whole-cell systems) is to use them in flow reactors.

283 By using the patch-clamp whole-cell technique, we examined the voltage- and time-

284 Using intact whole cells, the pressurized system was observed to rapi

285 Here, we have characterized nuclear and whole cell transcriptomes in mouse single neurons and pr

286 irmed a high concordance between nuclear and whole cell transcriptomes in the expression of cell type

287 ing DNA-based super-resolution microscopy in whole cells using standard instrumentation.

288 Such data are not available from LMICs using whole-cell vaccines in a primary schedule without booste

289 se questions, we performed two-photon guided whole-cell Vm recordings from primary visual cortex laye

290 Whole-cell voltage clamp recordings were then made from

291 trode arrays and ex vivo brain slices, using whole-cell voltage clamp.

292 Whole-cell voltage-clamp analyses in HEK293 cells demons

293 4 oocytes per CSF sample was performed in a whole-cell voltage-clamp assay.

294 nts in heterologous expression systems using whole-cell voltage-clamp electrophysiology and immunohis

295 Whole-cell voltage-clamp recordings from cGOF or icGOF v

296 tifying potassium current was measured using whole-cell voltage-clamp recordings from KF and locus co

297 Whole-cell voltage-clamp recordings of mEPSCs in CA1 pyr

298 Whole-cell voltage-clamp recordings revealed that blocki

299 nses upon drug binding, whereby many tens of whole cells were imaged.

300 Acellular pertussis (aP) and whole-cell (wP) pertussis vaccines are presumed to have