ライフサイエンスコーパス: wild-type gene

1 lative to the same strain that expressed the wild type gene.

2 ognosis as compared to patients carrying the wild type gene.

3 mbination into the chromosome, replacing the wild-type gene.

4 oss of function as compared with that of the wild-type gene.

5 ained as plasmids unless complemented by the wild-type gene.

6 ed this deficiency in transfectants with the wild-type gene.

7 m mutation but from elevated expression of a wild-type gene.

8 s of the gene can reconstruct a functioning, wild-type gene.

9 y of the mutant gene was used to replace the wild-type gene.

10 nce by complementation with their respective wild-type gene.

11 zygotic for this change or those who had the wild-type gene.

12 restored by genetic complementation with the wild-type gene.

13 mutation was complemented in trans with the wild-type gene.

14 ns showed the same outcome as those with the wild-type gene.

15 600E) in the presence of 20-fold more of the wild-type gene.

16 ant dgt gene on the chromosome replacing the wild-type gene.

17 orm cannot accomplish many activities of the wild-type gene.

18 tably transfected derivatives expressing the wild-type gene.

19 , we isolated genomic and cDNA clones of the wild-type gene.

20 tandem repeat array inserted upstream of the wild-type gene.

21 P that alters the stop codon of an otherwise wild-type gene.

22 and complementation of this mutant with the wild-type gene.

23 ued by replacement of the cDNA copy with the wild-type gene.

24 restored upon complementation with the embC wild-type gene.

25 nsulin, respectively, than mice carrying the wild-type gene.

26 nal regulation is a critical function of the wild-type gene.

27 omplemented by a plasmid-encoded copy of the wild-type gene.

28 itivity was restored by complementation with wild-type genes.

29 defects by plasmid clones of the respective wild-type genes.

30 lly expressing the corresponding Arabidopsis wild-type genes.

31 ith loss of the CH-42 transgene and adjacent wild-type genes.

32 tivity to the same extent as the 196-residue wild-type gene 1.7 protein.

33 form dimers at salt concentrations where the wild-type gene 2.5 protein exists as a dimer.

34 itive mutant revealed a 21-bp duplication of wild-type gene 37 inserted into its C-terminal portion.

35 dual deletion strains with the corresponding wild-type gene abolished cisplatin resistance, confirmin

36 an adenoviral construct encoding the IKKbeta wild-type gene (Ad.IKKbeta-wt); controls received an ade

37 develop in people bearing one mutant and one wild-type gene allele.

38 ly mosaic worms, in which some cells carry a wild-type gene and others are homozygous mutant, can rev

39 ein was the same size as that encoded by the wild-type gene and that both the wild-type and mutated p

40 s caused by recombination events between the wild-type gene and the pseudogene(s).

41 ntervals: patients showing the best outcome (wild-type gene and unaltered protein), an intermediate o

42 ly acting exogenous genes, overexpression of wild-type genes, and Cre recombinase-mediated gene ablat

43 Whereas both mutant and wild-type genes appeared to be transcribed and spliced e

44 ed)1 mutants indicate that the corresponding wild-type genes are required to repress root phenylpropa

45 specific genetic interactions using a cloned wild-type gene as a starting point.

46 ing loss of function, many others maintain a wild-type gene but exhibit reduced p53 tumor suppressor

47 n was complemented by the plasmid-borne yDHS wild-type gene, but not by mutated genes encoding inacti

48 ve-site mutations, and overexpression of the wild-type gene, but not the catalytic-site mutant, parti

49 uch weaker, basal level transcription of the wild type gene by RNA polymerase II under non-heat shock

50 We cloned the wild-type gene by complementation of the temperature-sen

51 endonuclease gene; (3) back-crosses with the wild-type gene by ligation to the wild-type N-terminal o

52 Expression of the wild-type gene (CAMV35S::ECA1) reversed these conditiona

53 lectivity - one mutant in 1000 copies of the wild-type gene can be detected.

54 within BLM can occur to form a functionally wild-type gene capable of correcting the mutant phenotyp

55 Overexpression of the wild-type gene causes cell death and disrupts the normal

56 cterized HCMV strain containing the complete wild-type gene complement and promises to enhance the cl

57 Thus, HCMV containing a wild-type gene complement can be generatedin vitroby der

58 pase-resistant GATA-1 mutant, but not of the wild-type gene, completely restored erythroid expansion

59 e can be rescued by complementation with the wild-type gene, consistent with a function for AtNBS1 in

60 simplification allows one to deduce how the wild-type gene contributes to patterning the normal, mor

61 since they all contained both disrupted and wild-type gene copies, and expression of functional GbpB

62 urrent than coexpressing the mutant with the wild-type gene, demonstrating that the polymorphism resc

63 4.0-5.4 months), patients carrying the TLR4 wild type gene displayed cardiac recovery under intense

64 f active site mutants of DsbA instead of the wild-type gene do not produce this increase in yield.

65 plication of 270 kb that includes the entire wild-type gene encoding the tight junction protein TJP2

66 coenzyme A pathway intermediates or with the wild-type gene encP restored the formation of the benzoa

67 E RESPONSE1 and requires the function of the wild-type genes ETHYLENE INSENSITIVE2 (EIN2), EIN4, AUXI

68 Mutant strains, transformed with a copy of a wild-type gene, exhibited a macrophage survival level si

69 We found 17 modes of wild-type gene expression and refined them into 57 submo

70 ssion of the mutant allele without affecting wild-type gene expression could be a powerful new treatm

71 on of Mist1 in adult Mist1(KO) mice restored wild-type gene expression patterns in acinar cells.

72 chd mRNA into the early embryo, we restored wild-type gene expression patterns, and the resultant fi

73 The model is able to reproduce the wild-type gene expression patterns, as well as the ectop

74 The wild-type gene for alanine dehydrogenase, incorporated i

75 Replacement of five codons in the wild-type gene for antigen 85B increased recombinant pro

76 omes are prepared from s strain carrying the wild-type gene for PAP I (pcnB+).

77 Complementation of the cysA mutant with the wild-type gene from M. tuberculosis restored prototrophy

78 A cloned wild-type gene from this region, abgT (formerly ydaH) co

79 ene for damage avoidance, i.e., to produce a wild-type gene from two nonfunctional allelic copies of

80 ngth could be complemented by expressing the wild-type genes from a plasmid.

81 e assay, based on a visible test for loss of wild-type gene function at a single locus, golden, is re

82 d efficient method for obtaining clues about wild-type gene function.

83 ss morphological phenotypes, not because the wild-type gene functions pleiotropically in several sign

84 about 20-fold brighter fluorescence than the wild-type gene (gfp).

85 x Normal, suggesting that both copies of the wild-type gene had undergone RIP.

86 The complementing wild-type gene has been cloned and and shown to encode a

87 vity of a cdc14-1(ts) mutant nor replace the wild type gene in vivo, demonstrating that phosphatase a

88 ype that can be rescued by expression of the wild-type gene in clock neurons, indicating a role for S

89 s found the coding region unchanged from the wild-type gene in each case, but variation was observed

90 verified by complementation with the cloned wild-type gene in in vitro and in vivo assays.

91 the rad53 mutant, suggesting a role for the wild-type gene in maintaining chromosome integrity in th

92 , and phenotypic rescue by expression of the wild-type gene in rdn1 mutants.

93 The mutant strain was complemented with the wild-type gene in single copy.

94 Single mutants were complemented with the wild-type gene in trans, showing restoration of the wild

95 uld be complemented by the expression of the wild-type gene in trans.

96 eover, expressing GRK4gammaA142V but not the wild-type gene in transgenic mice produces hypertension

97 Expression of the wild-type gene in TSC2 mutant tumor cells inhibits proli

98 d by UUG was also able to substitute for the wild-type gene in vivo in yeast.

99 ied that it encodes C3H by expression of the wild-type gene in yeast.

100 this clone were made and used to replace the wild-type genes in the bacterial chromosome by marker ex

101 ene knocked out in the micronucleus but only wild-type genes in the polycopy somatic macronucleus.

102 ant was complemented with the representative wild-type genes in trans to restore expression of parent

103 eculiarity of TCRbeta, as we identified many wild-type genes, including one essential for NMD, that t

104 heme-dependent manner similar to that of the wild-type gene, indicating that control by those effecto

105 these regulatory sequences are removed, the wild-type gene is expressed at high levels.

106 detect 1 codon 61 mutant allele among 10,000 wild-type genes, is described.

107 This finding indicated that the wild-type gene junction has evolved to achieve the maxim

108 reby heterozygous coexpression of mutant and wild-type genes leads to more serious pathology than is

109 to understanding the mechanisms by which the wild-type genes lose activity.

110 ved cells transduced with virus carrying the wild-type gene maintained normal blood counts following

111 Experiments demonstrate that the wild-type gene Mop1 is required for establishment and ma

112 The wild-type gene, named mucK, was cloned into pUC18, and i

113 reports on the essentiality of both the yrdC wild-type gene of Escherichia coli and the SUA5 wild-typ

114 d-type gene of Escherichia coli and the SUA5 wild-type gene of Saccharomyces cerevisiae led us to rec

115 The present data show that the wild-type genes of two members of the Halloween family o

116 ype was rescued by the reintroduction of the wild-type gene on a plasmid, proving that the hyphal gro

117 the gene of interest and used to replace the wild-type gene on the chromosome by allelic exchange.

118 hese mutant alleles were used to replace the wild-type gene on the streptococcal chromosome, analysis

119 ectly test their function, we introduced the wild-type genes on high-copy plasmids into Escherichia c

120 osits are derived from precursors encoded by wild-type genes (perhaps influenced by alleles that are

121 allows AVP secretion, albeit less than with wild-type gene precursor.

122 The nature of the wild-type gene product at the mouse ichthyosis (ic) locu

123 ponding mutant, agp30, has revealed that the wild-type gene product is required in vitro for root reg

124 enotype of the digenic rga/gal-3 mutant, the wild-type gene product of RGA is probably a negative reg

125 Overexpression or misexpression of a wild-type gene product, however, can also cause mutant p

126 E. coli strains that allow depletion of the wild-type gene product.

127 with the function of the wild-type mtDNA or wild-type gene products.

128 rms are used to describe three attributes of wild-type gene products: their molecular function, the b

129 e phenotype of the deletion strains with the wild-type genes proves that the sensitivity of the strai

130 Complementation of an hp310 strain with the wild type gene restored lysozyme resistance.

131 n of the F1 DeltamcpC mutant XLF004 with the wild-type gene restored chemotaxis to cytosine.

132 t strain by chromosomal reintegration of the wild-type gene restored expression of this murein hydrol

133 Complementation of the deletion with the wild-type gene restored full virulence.

134 lementation of P. gingivalis FLL455 with the wild-type gene restored the level of NO sensitivity to a

135 ion of mutant lines with their corresponding wild-type genes restored picolinate auxin sensitivity.

136 Plasmids carrying the complementary wild-type genes restored the ability of mutant strains t

137 of the 1818(PE_PGRS)::Tn5367 mutant with the wild-type gene restores both aggregative growth (clumpin

138 any inconsistency between the entry and the wild type gene sequence.

139 Comparison of mutant and wild-type gene sequences in this region revealed a G-to-

140 e silencing of transcription from methylated wild-type gene sequences was demonstrated.

141 growth and a distinct color, but maintains a wild-type gene set and the capacity for photosynthesis.

142 Molecular analysis of the wild-type gene showed that this process involves gene co

143 2 gene revealed a pattern different from the wild-type gene, suggesting a mutation in the sod2 gene.

144 The respective wild-type genes suppress the peroxide sensitivities of s

145 We replaced the wild-type gene that encodes the NADP-dependent IDH of Es

146 revealed a G to A transition relative to the wild-type gene that would result in the substitution of

147 transferred to the chromosome, replacing the wild-type gene, the cells became inviable.

148 s the correction of a mutated allele back to wild-type ("gene therapy").

149 In contrast to the wild-type gene, this mutant has no effect on cell clonog

150 nabled us to measure the rate of loss of the wild-type gene through deletion, recombination, or gene

151 nuclease) cleaved the imperfectly hybridized wild-type gene together with all other mismatched sequen

152 valuation when compared with carriers of the wild type gene under the same treatment conditions.

153 les revealed that the ATG start codon of the wild-type gene was converted to the rare GTG start codon

154 Second, a wild-type gene was disrupted by the insertion of a marke

155 nd deficiency mapping, and the corresponding wild-type gene was isolated in a molecular walk.

156 The wild-type gene was restored in the mutant by homologous

157 However, in the wild type gene we have found that CPDs are efficiently r

158 ect that could be complemented by the cloned wild-type gene, we have designated EF1809 ebrA (enteroco

159 Mutant and wild-type genes were cloned into a Leishmania-specific v

160 sulted in the replacement of the rickettsial wild-type gene with a partially deleted pld gene.

161 R) in embryonic stem (ES) cells to replace a wild-type gene with a slightly modified one.

162 a complementing chromosomal insertion of the wild-type gene with its indigenous promoter at a second