ライフサイエンスコーパス: wobble

1 interacts with a newly resolved G.U reverse wobble.

2 ighboring base pairs on only one side of the wobble.

3 ls moved downward at low forces with minimum wobble.

4 al hydration sphere with the G25.U20 reverse wobble.

5 rse wobble with an isosteric A25.C20 reverse wobble.

6 lso a UAG nonsense suppressor via first base wobble.

7 CUG) anticodon stem that restrict first base wobble.

8 -derived metabolites required to confer tRNA wobbling.

9 )C nor the R(61,2)C adduct formed protonated wobble A.C hydrogen bonds.

10 unexpected result, however, is that a highly wobbled A.T base pair, which is ascribed here to a rare

11 )) were essential for Watson-Crick (AAA) and wobble (AAG) cognate codon recognition by tRNA(UUU)(Lys)

12 ifications also play a role in accommodating wobble, allowing a limited pool of tRNAs to recognize de

13 pe II tandem G.U pairs have a combination of wobble and bifurcated hydrogen bonds where the uracil 2-

14 The relative contribution of the neutral wobble and protonated Watson-Crick configurations to 2AP

15 ease mismatch discriminations (including T/G wobble and T/C mismatched base pairs) while maintaining

16 at an equilibrium exists between the neutral wobble and the protonated Watson-Crick structures.

17 guide-target interactions we introduced G:U wobbles and mismatches at various positions of the micro

18 ions, including combinations of multiple G:U wobbles and mismatches in the seed region, are admissibl

19 the rate-accuracy variation for 7 cognate, 7 wobble, and 56 near-cognate codon readings comprising ab

20 three mismatched base-pairs: an A+-C, a G-U wobble, and a sheared G-A base-pair and no looped out ba

21 and the MT wall should cause a Dam1 ring to wobble, and Fourier analysis of moving, ring-attached be

22 The anticodon wobble at position 34 and the nucleotide immediately 3'

23 Watson-Crick base pairing properties of the wobble base (and hence proper translation of the genetic

24 could be developed that would alter the tRNA wobble base base pairing properties.

25 rentially catalyzes the incorporation of the wobble base G, rather than the Watson-Crick base A, oppo

26 0 lies in close proximity to the P-site tRNA wobble base in order to satisfy a UV-induced photocrossl

27 We show that U:T wobble base interactions are critical to prevent extensi

28 is unable to rectify a break at the modified wobble base of tRNA(Glu(UUC)).

29 Trm9 methylates the uridine wobble base of tRNAARG(UCU) and tRNAGLU(UUC).

30 air (on the guanine containing strand) and a wobble base pair (on the strand containing the difluorot

31 containing a bulged nucleotide adjacent to a wobble base pair also was primarily affected by non-near

32 other hand, recognizes the TG mismatch as a wobble base pair and penetrates the DNA with three aroma

33 he templating base, Poliota accommodates the wobble base pair better than the Watson-Crick base pair.

34 he ribozyme and the data here show that this wobble base pair destabilizes neighboring base pairs on

35 The G.U wobble base pair formed between a (15)N-labeled strand a

36 econciled by possible tautomerization of the wobble base pair in mRNA-tRNA.

37 laRS) has depended predominantly on a single wobble base pair in the acceptor stem, G3*U70, mainly on

38 Importantly, the mcm(5)s(2)U(34).G(3) wobble base pair is in the Watson-Crick geometry, requir

39 ent upon the position and orientation of the wobble base pair relative the bulged nucleotide.

40 om A-form helix occur where the guanine of a wobble base pair stacks over a purine from the opposite

41 -thymine mispairs may be associated with its wobble base pair structure.

42 Mutation of the U50.G64 wobble base pair to C50:G64 or U50:A64 base pairs increa

43 nt for elongation and that it is the U50.G64 wobble base pair, located at the same position in the TP

44 or both of the bulge nearest neighbors was a wobble base pair, the free energy increment for insertio

45 n interacts with N7 of the cleavage site G.U wobble base pair.

46 e modified base pair in the structure adopts wobble base pairing (hydrogen bonds between [POB]dG(N1)

47 er, interactions between adjacent codons and wobble base pairing are key.

48 The former is most likely due to wobble base pairing between ClU and G, which may be more

49 The helical distortions and wobble base pairing induced by the covalent binding of P

50 G.U wobble base pairing is tolerated as a match for both RNA

51 However, the wobble base pairing, where T pairs with G instead of A,

52 tion of miRNA base pairing or by creation of wobble base pairing.

53 Adjacent GxU wobble base pairs are frequently found in rRNA.

54 G.U wobble base pairs are the most common and highly conserv

55 side chain plays a pivotal role in excluding wobble base pairs between template pyrimidines and purin

56 The wobble base pairs contribute to the catalytic rate enhan

57 ostatic potential at the major groove of G.U wobble base pairs embedded in RNA helices, suitable for

58 reases the stability of duplexes closed with wobble base pairs in an idiosyncratic manner.

59 ructure and the importance of two tandem G:U wobble base pairs in the template domain were studied by

60 at the negativity at the major groove of G.U wobble base pairs is determined by the combined effect o

61 d melting temperature for duplexes closed by wobble base pairs with 3' single or double-nucleotide ov

62 polymerase active site and the asymmetry of wobble base pairs, provides a plausible explanation for

63 However, the wobble base pairs, where U in RNA (or T in DNA) pairs wi

64 n both sides by cis Watson-Crick G/C and G/U wobble base pairs.

65 While existing data link wobble base U34 modifications to translation of function

66 a G.C replacement were reversed by a distal wobble base-pair in the anticodon helix.

67 However, G:U wobble base-pairing in this region interferes with activ

68 Guanine-uracil (G.U) wobble base-pairs are a detrimental lesion in DNA.

69 Previous investigations have shown that such wobble base-pairs are more prone to base-opening than th

70 d, to a lesser extent, guanine residues from wobble base-pairs in hairpin stems.

71 ed 5ns molecular dynamics simulations on G.U wobble base-pairs in two different sequence contexts, TG

72 Incorporation of 6MI yields wobble base-pairs that open more readily than their guan

73 rmediate that involves DNA-base flipping and wobble base-pairs.

74 iting stimulates conversion of A to I at the wobble base.

75 one containing a central G-T mismatched or "wobble" base pair, and one in which the thymine in this

76 n an accurate but inefficient manner with a "wobble" base pairing between C and O(6)-MeG.

77 oaching that of its natural analogue, a G-T (wobble) base pair.

78 and molecular dynamics (MD) study of the G/U wobble basepairs in the ribosome based on high-resolutio

79 sults in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C t

80 imental systems have shown that systems may 'wobble' before a critical transition.

81 nated at the N1 position to form stabilizing wobble CA+ pairs adjacent to a sheared GA or AA pair.

82 ree-energy model, including stabilization by wobble CA+ pairs, is derived for predicting stabilities

83 cated these tRNA modifications in modulating wobbling capacity and translation efficiency, their exac

84 the 5' single terminal overhangs adjacent to wobble closing base pairs are also presented.

85 For example, early hypoxia increases wobble cmo(5)U in tRNA(Thr(UGU)), which parallels transl

86 y of discrimination against near-cognate and wobble codon readings increased toward the maximal asymp

87 In contrast, other modifications expand wobble codon recognition, such as U*U base pairing, for

88 iscriminating between the correct cognate or wobble codons and the incorrect near-cognate codons (e.g

89 ance to tRNA's ability to decode cognate and wobble codons become apparent.

90 xhibited high affinities for its cognate and wobble codons GUA and GUG, and for GUU in the A-site of

91 ient and accurate recognition of cognate and wobble codons.

92 igase junction is predominantly in a neutral wobble configuration and is poorly ligated.

93 ] internal loop, the GU pairs form canonical wobble configurations with two hydrogen bonds, whereas i

94 atch is also poised for catalysis but in the wobble conformation seen in other studies, indicating th

95 he mismatched C(5).A(16) pair existed in the wobble conformation, with the C(5) imino nitrogen hydrog

96 TIPRL also makes unusual wobble contacts with the scaffold subunit, allowing TIPR

97 t sulfation patterns, which are termed here "wobble CS/DS oligosaccharide motifs," and induce signali

98 CAA-decoding tRNA(Gln)(UUG), an inefficient wobble-decoder of CAG.

99 NMR relaxation dispersion, we show here that wobble dG*dT and rG*rU mispairs in DNA and RNA duplexes

100 R relaxation dispersion recently showed that wobble dG.dT and rG.rU mismatches in DNA and RNA duplexe

101 ition of thymidine, we have investigated the wobble discrimination by manipulating the steric and ele

102 hat the major groove edge of an isolated G.U wobble displays distinctly enhanced negativity compared

103 The extent of the local ring dynamic wobble does not increase, and may decrease slightly, whe

104 ion of the (31)P, while a slower rotation or wobble dominates the relaxation of the carbonyl carbon b

105 her restricting codon recognition, expanding wobble, enabling translocation, or maintaining the messe

106 not adjacent to an A.A pair forming a static wobble G.U pair.

107 er of non-canonical base pairs including the wobble G.U pairs were identified.

108 s indicate that the ClU-G base pair adopts a wobble geometry at neutral pH, similar to a T-G mispair.

109 s a Watson-Crick-like geometry rather than a wobble geometry, suggesting that the enol tautomeric for

110 urine-pyrimidine mispairs adopt the expected wobble geometry, the difference between the two polymera

111 ing the number of strong (GC), weak (AU) and wobble (GU) base pairs to lie in a certain range, the RN

112 Removal of the wobble hydrogen bonds in U:dT recovers a strong response

113 The Modified Wobble Hypothesis proposed in 1991 that specific modific

114 One foundation of Crick's Wobble Hypothesis was that a near-constant geometry of c

115 d in the third base-pair, giving rise to the Wobble Hypothesis.

116 ( approximately 2 mus), while simultaneously wobbling in a cone of semiangle 30-55 degrees centered a

117 Orientational relaxation is analyzed with a wobbling-in-a-cone model describing restricted orientati

118 two periods of restricted angular diffusion (wobbling-in-a-cone) followed by complete orientational r

119 ing deprotonated G-C structure is a "reverse wobble" incorporating two N-H...N hydrogen bonds.

120 d Mg(2)(+) positioned at the G25.U20 reverse wobble is catalytic and could serve as a Lewis acid, a B

121 ely because of averaging by fast motions and wobble; it is tentatively estimated to be 1 x 10(7) s(-1

122 es are attributed to inversion via a lateral wobble mechanism with DeltaH++ = 6 kcal x mol(-1) and De

123 These results support and extend our "wobble" model for CP binding to the actin filament, in w

124 Unlike classical "wobble" models, our analyses showed that three of four p

125 The human wobble modification has a less dramatic loop remodeling

126 Wobble modifications, t(6)A37, and magnesium each make u

127 d including an inertial motion, a restricted wobbling motion of approximately 3 ps, and complete rand

128 cence anisotropy decay and internal angular 'wobbling' motion measurements of 2-AP within these alter

129 LC activity, and slows nanosecond rotational/wobbling motions of both phospholipid headgroups, as ind

130 sicles, we found large-amplitude, rigid-body wobbling motions on the nanosecond time scale relative t

131 The time scales and amplitudes of these "wobbling" motions are characterized by effective correla

132 entifies three global motions: torsional and wobbling movements, en bloc, between the alpha- and beta

133 th longer inserts was made more efficient by wobble-mutagenizing both the inner repeat and the exogen

134 overcome by expression of a cDNA encoding a wobble mutant of FBL2.

135 lowing introduction of a DLX5 cDNA harboring wobbled mutations at the shRNA-targeting sites.

136 n 1991 that specific modifications of a tRNA wobble nucleoside shape the anticodon architecture in su

137 The wobble nucleotide positions of each codon were replaced,

138 unless an adjustable amplitude of azimuthal wobble of the methylene blue was admitted.

139 ted as originating from two types of motion: wobbling of tryptophan side-chains relative to the prote

140 tabilizing effects induced by the tandem G.U wobbles on the double-stranded structure of this stem, w

141 w these hexameric ATPases interact with and "wobble" on top of the heptameric 20S proteasome.

142 The cis-5R,6S Tg lesion is in the wobble orientation such that Tg(6) O(2) is proximate to

143 dTTP opposite template N2,N2-Me2G revealed a wobble orientation.

144 a simple average of a back-and-forth display wobble over time.

145 ompanied by formation of an internal AH(+).C wobble pair [Siegfried, N.

146 mations for the P1.1 stem, the cleavage site wobble pair and the A-minor motif of the catalytic trefo

147 These conserved sequences include a u-G wobble pair at the 5' splice site and a guanosine in the

148 rcus A58 in the J4/5 region contacts the G.U wobble pair at the cleavage site in the P1 helix, and Az

149 ave hypothesized to discriminate against U/G wobble pair by tailoring the steric and electronic effec

150 The wobble pair existed as a mixture of protonated and nonpr

151 he presence of AFB(1) did not interfere with wobble pair formation at the mismatched site.

152 rms a stem-loop structure stabilized by a GU wobble pair formed by two of the five unpaired residues

153 ed pH-dependent formation of the A2450+C2063 wobble pair has made it a potential candidate for the pH

154 an extensive in vivo analysis of the distal wobble pair in alanine tRNA and report that it does not

155 st a previously unrecognized role of the G.U wobble pair in self-splicing: breaking cooperativity in

156 trogen was similar to that of the C(5).A(16) wobble pair in the corresponding duplex not adducted wit

157 variation in recognition was that the G2.U71 wobble pair of spirochete tRNALys acts as antideterminan

158 termini of these siRNAs with a terminal G-U wobble pair or a carefully selected pair of terminal asy

159 acceptance by the tRNA, but that C.A and G.A wobble pair replacements preserve acceptance.

160 suggested that protonation of the C(5).A(16) wobble pair should shift C(5) toward the major groove an

161 l structure of a DNA duplex containing a T:G wobble pair shows similar structural changes imposed by

162 x G80), and disrupts a protonated C67 x A79 wobble pair that forms in the wild-type structure.

163 esulting in the formation of the U2506*G2583 wobble pair that was attributed to a catalytically inact

164 This novel mechanism enables the single wobble pair to dominantly determine the specificity of t

165 rimental evidence and suggest how the distal wobble pair was incorrectly analyzed.

166 e isosteric, but pH-independent, G2450*U2063 wobble pair, and 50S subunits containing the mutations w

167 duplex r(guguuuac)/r(guaggcac) with a tandem wobble pair, G.G/U.U (motif III), to compare it with U.G

168 n the Watson-Crick pairs and 15 span the G:U wobble pair, including two interesting arrangements with

169 disrupts several tertiary contacts with the wobble pair, the assignment of A2450 as the active site

170 he DNAzyme confirm the importance of the G*T wobble pair, the two loops and the intervening stem in m

171 d, a pK(a) of 8.0 is observed for the A(+).C wobble pair, which represents an especially large shift

172 en the geometries of rF:G and the native U:G wobble pair.

173 instead of the two expected for a canonical wobble pair.

174 sual stacking interactions but do not form a wobble pair.

175 sistent with the possible formation of a G.U wobble pair.

176 base pair, while discriminating against U/G wobble pair.

177 form stem that contains a pH-sensitive A(+)C wobble pair.

178 y the 5'UAGG/3'GGAU loop adopts canonical UG wobble pairing (cis Watson-Crick/Watson-Crick), with AG

179 The wobble pairing and disorder of the Tg.G mismatch correla

180 Crystal structures reveal wobble pairing between C and O(6)-BzG.

181 cted to the complementary base plus a single wobble pairing for amino acids with twofold degenerate c

182 ut the glycosyl bond and forms a less stable wobble pairing interaction with guanine.

183 The U/G wobble pairing is ubiquitous in RNA, especially in non-c

184 G:T and A:C wobble pairing leads to a high error rate, but the modif

185 dified nucleoside 2-thiothymidine suppresses wobble pairing.

186 y crystallographic studies, which show that "wobble" pairing occurs between C and O6-MeG.

187 We believe that wobble-pairing between T and G is responsible for misinc

188 cal/mol on eight stacked pairs involving G-U wobble pairs and 0.99 kcal/mol on seven stacked pairs in

189 a identify a set of mutations, including G-U wobble pairs and nucleotide mismatches in the 5' hairpin

190 ut for both the protonated and nonprotonated wobble pairs at C(5).A(16).

191 This study suggests that protonated A(+).C wobble pairs exist in DNA under biologically relevant co

192 racyclines preferentially bind within the UG wobble pairs flanking an asymmetrically bulged C-residue

193 Among protonated mismatches, A(+).C wobble pairs form near physiological pH and have relativ

194 d ionic strength on pK(a) shifting in A(+).C wobble pairs in DNA.

195 binding site that consists of the tandem G:U wobble pairs in P1 and consecutive G:U and U:A pairs in

196 search for exact matches while including G-U wobble pairs or employ simplified energy models, we pres

197 x r(GGGCGCUCC)2with non-adjacent G*U and U*G wobble pairs separated by four Watson-Crick base pairs h

198 stable structure consisting of conserved UG wobble pairs, a folded 2X2 (GU/UA) internal loop, a UU b

199 Two wobble pairs, A2453-C2499 and A2450-C2063, have been pro

200 ismatches, including nearly isoenergetic RNA wobble pairs, can be efficiently rejected with discrimin

201 ino, AU reverse Hoogsteen, and the GU and AC wobble pairs.

202 stabilize U:A base pairs and destabilize U:G wobble pairs.

203 n to stabilize U:A pairs and destabilize U:G wobble pairs.

204 ryotes) that contain an adenosine (A) at the wobble position (position 34).

205 tion at the 2'-hydroxylribosyl moiety in the wobble position (Um34) of Sec tRNA([Ser]Sec), and conseq

206 ional modification, 5-formylcytidine, at the wobble position 34 (f(5)C(34)), and a cytidine substitut

207 e post-transcriptional modifications at tRNA wobble position 34 and purine 37, 3'-adjacent to the ant

208 e-5-oxyacetic acid (cmo (5)U 34) is found at wobble position 34 in a single isoaccepting tRNA species

209 e show that the complete modification at the wobble position 34 is 5-carboxyaminomethyl-2-thiouridine

210 Post-transcriptional modifications at tRNA's wobble position 34, especially modifications of uridine

211 odified 2-thiouridine (s(2)U) derivatives at wobble position 34.

212 n usage displays the expected GC bias in the wobble position and is consistent with a highly acidic p

213 Modifications at the anticodon's wobble position are required for recognition of rarely u

214 In most organisms, the C34 wobble position in these tRNA(Ile) precursors is rapidly

215 t into longstanding questions regarding both wobble position modification and the nearly ubiquitous t

216 Queuosine (Q) was discovered in the wobble position of a transfer RNA (tRNA) 47 years ago, y

217 Here, we show (i) that unlike U34 at the wobble position of all B. subtilis tRNAs of known sequen

218 The 2-thiouridine (s(2)U) at the wobble position of certain bacterial and eukaryotic tRNA

219 methylcarboxymethyl uridine (mcm(5)U) at the wobble position of certain tRNAs, a critical anticodon l

220 on of the modified base queuine (Q) into the wobble position of certain tRNAs.

221 by examining the level of divergence at the wobble position of codons.

222 ce was also noted when only mutations in the wobble position of degenerate codons were considered.

223 inomethyluridine (taum(5)U) in the anticodon wobble position of five mitochondrial tRNAs.

224 uanosine nucleoside located in the anticodon wobble position of four amino acid-specific tRNAs.

225 droxyuridine into 5-oxyacetyl uridine at the wobble position of multiple tRNAs in Gram-negative bacte

226 is almost universally found in the anticodon wobble position of specific tRNAs.

227 te codon AAC, has the modified base Q at the wobble position of the anticodon (5' QUU 3') and it has

228 ed in tRNA: queuosine, which is found at the wobble position of the anticodon in bacterial and eukary

229 hat is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containin

230 enouridine (mnm(5)se(2)U), is located at the wobble position of the anticodons of tRNA(Lys), tRNA(Glu

231 terferes with the eRF1 decoding of the third/wobble position of the stop codon set in the unfavorable

232 Uridine 34 (U34) at the wobble position of the tRNA anticodon is post-transcript

233 ological importance of A-to-I editing at the wobble position of tRNA.

234 In such a strain, C34 at the wobble position of tRNA2(Ile) is expected to remain unmo

235 a modified C (lysidine or agmatidine) at the wobble position of tRNA2(Ile) to base pair specifically

236 l deamination of adenosine to inosine at the wobble position of tRNAs and is necessary to permit a si

237 Enzyme-mediated modifications at the wobble position of tRNAs are essential for the translati

238 e deamination of adenosine to inosine at the wobble position of tRNAs.

239 ng in formation of 5-oxyacetyluridine at the wobble position of tRNAs.

240 modification occurs in tRNAs from a G in the wobble position to Queuosine that changes optimal bindin

241 The anticodon wobble position U(34) nucleobase in ASL(Lys3)(UUU)-t(6)A

242 Sec) by methylation of the nucleoside in the wobble position was repressed.

243 C; (c) an arginine tRNA with Inosine in the wobble position which reads CGU, CGC, and CGA bypasses m

244 by the nucleotide flexibility of their codon-wobble position(s).

245 he modifications that occur at the first, or wobble position, of tRNA's anticodon and those 3'-adjace

246 the modified nucleotide queuosine (Q) at the wobble position, thereby preventing protein synthesis an

247 on of tRNA(Leu(CAA)) containing m(5)C at the wobble position, which causes selective translation of m

248 ional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)).

249 m and loop of human tRNA(Lys1,2)(CUU) with a wobble position-34 C bound AAG, but did not wobble to AA

250 f cytidine, 5-formylcytidine (f(5)C), at the wobble position-34 of human mitochondrial tRNA(f5CAU)(Me

251 rs in the absence of modifications at either wobble position-34 or the conserved purine-37, 3'-adjace

252 ypermodified nucleoside queuosine (Q) at the wobble position.

253 ducing mature tRNAs without adenosine at the wobble position.

254 nate tRNAs with a mismatch only at the third/wobble position.

255 ifications to uridine in the tRNA anticodon 'wobble' position in both yeast and higher eukaryotes.

256 w that modified nucleosides at the first, or wobble, position of the anticodon and 3'-adjacent to the

257 The third, or "wobble," position of the codon is free to accommodate ce

258 Wobble positions are inherently unselected since regardl

259 dified RNA base that replaces guanine in the wobble positions of 5'-GUN-3' tRNA molecules.

260 ine (Q) is a hypermodified base found in the wobble positions of tRNA Asp, Asn, His, and Tyr from bac

261 fication of certain tRNAs in their anticodon wobble positions with queuine.

262 r (3) mismatches at four or more consecutive wobble positions.

263 However, this G x A pair is flanked by G x U wobbles, rather than an unpaired wedge.

264 discrimination against the formation of the wobble (Se)T/G base pair.

265 tion algorithms, are identified by the codon-wobble signatures of invariant amino acids.

266 wo unequal kinks (17 and 11 degrees ) at the wobble sites and a third kink at the central G5 site whi

267 structural and kinetic characterization of a wobble-specific deaminase.

268 The existence of the wobble state may be important for actin dynamics in cell

269 posal that G.fC and G.caC base pairs adopt a wobble structure that is recognized by TDG.

270 tomer, a protonated base pair, and a neutral wobble structure.

271 e hypothesis that the G690:U697 pair forms a wobble structure.

272 upon dsRNA binding and that canonical or GU-wobble substitutions produce dsRNA mutants that retain e

273 The internal AH(+).C wobble system further allowed us to parse energetic cont

274 0 self-complementary RNA duplexes containing wobble terminal base pairs with all possible 3' single a

275 The most common are the GU wobble, the Sheared GA pair, the Reverse Hoogsteen pair

276 wobble position-34 C bound AAG, but did not wobble to AAA, even when the ASL was modified with t(6)A

277 pecially modifications of uridine 34, enable wobble to occur.

278 ble anticodons indicating widespread use of 'wobble' tRNAs.

279 The S6G.T structures exhibit a wobble-type base pairing at the lesion site, with thymin

280 shows that the CACG tetraloop is closed by a wobble U.G base pair.

281 the standard Watson-Crick (C:G and U:A) and wobble U:G conformations, an analysis of the base-pair t

282 lical stem capped by a UACG tetraloop with a wobble UG closing base pair.

283 odifications of transfer RNAs (tRNAs) at the wobble uridine 34 (U34) base are highly conserved and co

284 sic region in Elp1 may be essential for tRNA wobble uridine modification by acting as tRNA binding mo

285 egulation, ubiquitination and cytosolic tRNA wobble uridine modification via 5-methoxycarbonylmethyl-

286 s essential for Elongator's function in tRNA wobble uridine modification.

287 on of translation elongation factor eEF2 and wobble uridine modifications of tRNAs.

288 oxycarbonylmethylation, respectively, of the wobble uridine of cytoplasmic (tK(UUU)), (tQ(UUG)), and

289 y directly controls the thiolation status of wobble-uridine (U34) nucleotides present on lysine, glut

290 M1 pathway responsible for the thiolation of wobble uridines in cytoplasmic tRNAs tK(UUU), tQ(UUG) an

291 fine-tuned its specificity to correlate with wobble versus nonwobble positions across that sequence a

292 the actin filament, and bound CP is able to wobble when attached only via its mobile beta-subunit te

293 stability, and disfavored by external A(+).C wobbles, which have high folding cooperativities but mak

294 pK(a) shifting is favored by internal A(+).C wobbles, which have low cooperativities of folding and m

295 of the hind legs with a slight side-to-side wobble while walking.

296 g(2)(+) ion, we replaced the G25.U20 reverse wobble with an isosteric A25.C20 reverse wobble.

297 G.U wobbles with distinct widening have similar major groove

298 ly active, demonstrating that AAA+ unfoldase wobbling with respect to 20S is not required for functio

299 g principal order parameter Szz for overall "wobble" with respect to the membrane normal (molecular z

300 Wobble, with D(perpendicular)= 1-2 x 10(8) s(-1), is the