ライフサイエンスコーパス: zeatin

1 he maize (Zea mays) cisZOG1 gene prefers cis-zeatin.

2 s of 46 and 96 microM, respectively, for cis-zeatin and a pH optimum of 7.5.

3 Both zeatin and BR induce dramatic changes in signaling and m

4 is of plant responses to two plant hormones, zeatin and brassinosteroid (BR).

5 ighlighted the largely differing response to zeatin and brassinosteroid by the metabolic pathways in

6 ory elements in plants and suggests that cis-zeatin and derivatives may be more important in cytokini

7 ed levels of other plant hormones, including zeatin and indole-3-acetic acid, are observed in BGL-1 l

8 Recent reports of cis-zeatin and its derivatives as the predominant cytokinin

9 ted mostly at the trans isomer, although cis-zeatin and its riboside occur as major components in som

10 The AHK4 receptor responded to trans-zeatin and m-topolin, while the ZmHK1 receptor responded

11 ile the ZmHK1 receptor responded also to cis-zeatin and o-topolin.

12 he maize enzyme recognizes as substrates cis-zeatin and UDP-glucose but not cis-ribosylzeatin, trans-

13 the formation of O-xylosylzeatin from trans-zeatin and UDP-xylose in immature seeds of Phaseolus vul

14 Glycosyl conjugates of zeatin are found in many plant tissues and are considere

15 unatus ZOG1 gene has high affinity for trans-zeatin as the substrate, whereas the enzyme encoded by t

16 ng N(6)-(delta(2)-isopentenyl)adenine, trans-zeatin, benzyladenine, kinetin, and thidiazuron inhibite

17 The enzyme is inhibited by zeatin, by 2,4-dichlorophenoxy-acetic acid, by IAA-myo-i

18 cis-Zeatin, cis-zeatin riboside, and their O-glucosides were

19 treatment, but 2,3,5-triiodobenzoic acid and zeatin enhanced transcript accumulation after 30 d in ro

20 The O-glucoside of zeatin, found in all plants examined, is considered to b

21 cis-Zeatin has traditionally been viewed as an adjunct with

22 g the formation of O-glycosyl derivatives of zeatin have been characterized, O-glucosyltransferase an

23 Biosynthesis and metabolism studies of zeatin have been directed mostly at the trans isomer, al

24 onjugants, confirmed by PCR, did not contain zeatin in their tRNAs and did not secrete zeatin into th

25 in zeatin in their tRNAs and did not secrete zeatin into the medium, findings which are consistent wi

26 trans-Zeatin is a major and ubiquitous cytokinin in higher pla

27 clear indication that O-glucosylation of cis-zeatin is a natural metabolic process in maize.

28 Zeatin is a naturally occurring cytokinin.

29 Zeatin is rapidly metabolized to O-xylosylzeatin in Phas

30 Zeatin is the most active and ubiquitous form of the nat

31 Zeatin is the most active and ubiquitous of the naturall

32 are consistent with the hypothesis that all zeatin is tRNA derived rather than synthesized de novo.

33 We identified the zeatin isolated as the trans isomer by HPLC and by a rad

34 ing an O-glucosyltransferase specific to cis-zeatin lends further support to this view.

35 rom Agrobacterium, which primarily increases zeatin levels, Sho expression in petunia and tobacco esp

36 Recently, the ZOG1 gene (zeatin O-glucosyltansferase) was isolated from P. lunati

37 The enzyme UDPglucose:zeatin O-glucosyltransferase (EC 2.4.1.203) was previous

38 e sequence of the gene ZOG1 encoding a trans-zeatin O-glucosyltransferase from Phaseolus (EC ), a cis

39 Zeatin O-xylosyltransferase (EC 2.4.2.-) mediates the fo

40 The zeatin O-xylosyltransferase mediating this conversion, a

41 Based on the ZOG1 sequence, the ZOX1 gene (zeatin O-xylosyltransferase) was cloned from P. vulgaris

42 -fold in the presence of exogenously applied zeatin-O-glucoside conjugate, indicating the release of

43 Glucosides of trans-zeatin occur widely in plant tissues, formed either by O

44 produced pseudonodules after treatment with zeatin or 2,3,5-triiodobenzoic acid, an auxin transport

45 e treated with exogenous cytokinin (1 microM zeatin) or auxin (30 nM 2,4-dichlorophenoxyacetic acid).

46 UDP-glucose but not cis-ribosylzeatin, trans-zeatin, or trans-ribosylzeatin.

47 To confirm that the secreted zeatin originated from tRNA, we mutated the miaA gene of

48 ification, we obtained 22 to 111 ng of trans-zeatin per liter from culture filtrates of four PPFM lea

49 re filtrates, suggesting that secreted trans-zeatin resulted from tRNA turnover rather than from de n

50 metabolic processes within the nodule (e.g., zeatin, riboflavin, and purine synthesis).

51 mutation does not cause a decrease in the CK zeatin riboside in the xylem sap or a strong increase in

52 igher levels of trans-zeatin riboside, trans-zeatin riboside monophosphate and isopentenyladenine 9-g

53 Smaller and variable amounts of trans-zeatin riboside were also recovered.

54 IAA-myo-inositol and IAA-glucan, but not by zeatin riboside, and only weakly by gibberellic acid, ab

55 cis-Zeatin, cis-zeatin riboside, and their O-glucosides were detected in

56 -D plants accumulated higher levels of trans-zeatin riboside, trans-zeatin riboside monophosphate and

57 g very high levels of the O-glucoside of cis-zeatin riboside.

58 cubation period and the use of the cytokinin zeatin riboside.

59 Whether cis-zeatin serves as a precursor to the active trans-isomer

60 These zeatin-specific genes and their promoters will be useful

61 cosyltransferase from Phaseolus (EC ), a cis-zeatin-specific O-glucosyltransferase was isolated from

62 For zeatin, the metabolic pathways in sucrose and starch bio

63 mbinant protein efficiently converts labeled zeatin to O-glucosylzeatin and has properties similar to

64 ering profiles were indicative of additional zeatin-type CKs in decapitated stems being supplied by r

65 strongly impairs the translocation of trans-zeatin (tZ)-type cytokinins from roots to shoots, thereb

66 igenic preprotein was processed, and labeled zeatin was converted to O-xylosylzeatin in transgenic pl

67 trans-Zeatin was recovered from tRNA hydrolysates in addition

68 Although the first naturally produced CK, zeatin, was isolated almost four decades ago, no endogen

69 WT roots and shoots, and growth responses to zeatin were comparable.