ライフサイエンスコーパス: zinc finger nuclease

1 ough linked protein domains (e.g. TALENs and zinc-finger nucleases).

2 th activity on par with a homodimeric Zif268 zinc-finger nuclease.

3 we compared with the well-established 'QQR' zinc-finger nuclease.

4 integrating RNA destabilizing elements using zinc finger nucleases.

5 a cells by targeted mutation of exon 8 using zinc finger nucleases.

6 ogenous Ig loci were silenced using designer zinc finger nucleases.

7 on of alpha or beta TCR chains with designer zinc finger nucleases.

8 t HIV Rev protein M10, fusion inhibitors and zinc-finger nucleases.

9 ation can now be reliably accomplished using zinc-finger nucleases.

10 may be applied to correction of X-CGD using zinc finger nuclease and patient iPSCs.

11 To this end, we developed designer zinc finger nucleases and employed a "hit-and-run" appro

12 er other sequence-specific nucleases, namely zinc finger nucleases and meganucleases, lies in their e

13 to other customizable endonucleases, such as zinc finger nucleases and transcription activator-like e

14 efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like e

15 Tev-LHEs are distinct from the FokI-derived zinc-finger nuclease and TAL effector nuclease platforms

16 of the nuclear genome by approaches such as zinc-finger nucleases and homologous recombination.

17 ed the Plasmodium falciparum K13 locus using zinc-finger nucleases and measured ring-stage survival r

18 porter gene carried a recognition site for a zinc-finger nuclease, and protoplasts from each tobacco

19 equency and spectrum of restriction-enzyme-, zinc-finger-nuclease-, and RAG-induced DSBs.

20 for the wild-type allele were edited using a Zinc Finger Nuclease approach.

21 Zinc finger nucleases are artificial restriction enzymes

22 Zinc-finger nucleases are chimeric proteins consisting o

23 codons and exerting control by microRNAs or zinc finger nucleases--are providing new ways of control

24 g DSBs (5' DSBs) in yeast using an optimized zinc finger nuclease at an efficiency that approached HO

25 Zinc finger nucleases can be designed to stimulate homol

26 Furthermore, we demonstrate that zinc finger nucleases can eliminate HLA-A expression fro

27 Engineered zinc finger nucleases can stimulate gene targeting at sp

28 double-strand break induced by the resulting zinc-finger nuclease can create specific sequence altera

29 The DNA-binding domain encoded by zinc-finger nucleases can be engineered to recognize a v

30 n vivo applications and to determine whether zinc finger nucleases create undesired genomic instabili

31 We show that zinc-finger nucleases designed against an X-linked sever

32 The clrn1 mutants generated by zinc finger nucleases displayed aberrant hair bundle mor

33 Although zinc-finger nucleases enable a variety of genome modific

34 assist selection of rare targeted clones and zinc finger nucleases engineered to specifically stimula

35 ial fingers can be used to create functional zinc finger nucleases for editing vertebrate genomes.

36 ising the possibility of strategies based on zinc-finger nucleases for the treatment of disease.

37 Here, we demonstrate that engineered zinc-finger nucleases function effectively in somatic ce

38 emonstrate that chromosome breaks created by zinc-finger nucleases greatly enhance the frequency of l

39 Zinc finger nuclease in-embryo editing of the RELA locus

40 Gene targeting by zinc-finger nucleases in one-cell embryos provides an ex

41 Here we apply zinc-finger nucleases in one-cell mouse embryos to gener

42 as an anticancer strategy, we disrupted, via zinc finger nucleases, MCT4 and BASIGIN genes in colon a

43 d mitochondrial DNA (mtDNA) damage and after zinc finger nuclease-mediated gene mutation correction,

44 Zinc finger nuclease-mediated gene targeting of a single

45 ntal requirement for Rest in zebrafish using zinc-finger nuclease-mediated gene targeting.

46 Zinc-finger nuclease-mediated targeted gene deletion of

47 ted a Go-opsin1 knockout Platynereis line by zinc-finger-nuclease-mediated genome engineering.

48 as motor and behavioural function in a novel zinc-finger nuclease model of RTT utilizing both male an

49 By combining zinc finger nuclease mRNA delivery with AAV6 delivery of

50 strate that temporally optimized delivery of zinc finger nuclease mRNA via electroporation and adeno-

51 Use of the mitochondrially targeted zinc finger-nuclease (mtZFN) results in degradation of m

52 ns and in cells genetically engineered using zinc-finger nucleases, single-nucleotide changes occur f

53 ines made deficient in O-glycan extension by zinc finger nuclease targeting of a key gene in O-glycan

54 hrombin III gene (at3) in zebrafish by using zinc finger nuclease technology.

55 mutations into the zebrafish fga gene using zinc finger nuclease technology.

56 developed a novel Bace1(-/-) rat line using zinc-finger nuclease technology and compared Bace1(-/-)

57 Using the zinc-finger nuclease technology, the generation of a tar

58 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drast

59 Dahl salt-sensitive (SS/JrHsdMcwi) rat using zinc-finger nuclease technology.

60 We have used this system to identify zinc-finger nucleases that exhibit high cleavage activit

61 are other potential biotechnologies, such as zinc finger nucleases, that could be also used for trans

62 In addition, we used Zinc finger nucleases to generate isogenic SHANK3 knocko

63 We used zinc finger nucleases to induce stable expression of hum

64 We have used zinc-finger nucleases to drive the simultaneous genetic

65 Here we use zinc-finger nucleases to generate targeted mutations in

66 Here we use zinc-finger nucleases to genetically modify the multidru

67 Using genome editing with zinc-finger nucleases to tag endogenous H3.3, we report

68 ogies for facile manipulation of the genome (zinc finger nucleases, transcription activator-like effe

69 Gene editing with zinc finger nucleases, transcription activator-like effe

70 e mainstream genome editing tools, including zinc finger nucleases, transcription activator-like effe

71 Zinc-finger nuclease, transcription activator-like effec

72 t expression of sequence-specific nucleases (zinc-finger nucleases, transcription activator-like effe

73 Here we develop a zinc-finger nuclease translocation reporter and screen f

74 Disruption of CCR5 using zinc finger nucleases was the first-in-human application

75 Using genome editing with zinc finger nucleases, we inserted a large, inducible XI

76 Using zinc finger nucleases, we show that disruption of the ze

77 Three-finger zinc finger nucleases were designed based on previously

78 To study the protein in more detail, zinc finger nucleases were used to edit the PORCN genomi

79 o wild-type (MSTN(+/+) ) rats, rats in which zinc finger nucleases were used to genetically inactivat

80 ome of the Dahl Salt-sensitive (S) rat using zinc-finger nucleases, wherein the mutant rat had a 17 b

81 ccelerated site-specific DNA cleavage by the zinc-finger nuclease, without enhancing off-target cleav

82 ement therapies that addresses these issues: zinc finger nuclease (ZFN) -mediated site-specific integ

83 activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) can be engineered into site-s

84 A transgene, flanked by zinc finger nuclease (ZFN) cleavage sites, was deleted f

85 activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology ena

86 The zinc finger nuclease (ZFN) mediated genomic editing gene

87 we report that combining electroporation of zinc finger nuclease (ZFN) mRNA with donor template deli

88 The emerging zinc finger nuclease (ZFN) technology facilitates gene t

89 The development of zinc finger nuclease (ZFN) technology has enabled the ge

90 FP respectively) by targeted integration via zinc finger nuclease (ZFN)--mediated homologous recombin

91 Initially, we injected zinc finger nuclease (ZFN)-encoding mRNA or DNA into bov

92 past two years, advances in transposon- and zinc finger nuclease (ZFN)-mediated gene knockout as wel

93 By combining zinc finger nuclease (ZFN)-mediated genome editing and i

94 ate a method for ligand-mediated delivery of zinc finger nucleases (ZFN) proteins using transferrin r

95 -mutant Meishan pigs with no marker gene via zinc finger nucleases (ZFN) technology.

96 ryonic stem cell (ESC)-based gene targeting, zinc-finger nuclease (ZFN) and transcription activator-l

97 This method uses a zinc-finger nuclease (ZFN) to create a site-specific dou

98 was rendered permanently dysfunctional by a zinc-finger nuclease (ZFN)--is safe.

99 three genes in human pluripotent cells using zinc-finger nuclease (ZFN)-mediated genome editing.

100 The technology of zinc finger nucleases (ZFNs) allows DNA double-strand br

101 Targeted endonucleases including zinc finger nucleases (ZFNs) and clustered regularly int

102 no-associated virus (AAV) vector delivery of zinc finger nucleases (ZFNs) and corrective donor templa

103 vectors by using a combination of engineered zinc finger nucleases (ZFNs) and homing endonucleases.

104 Here we show that a combination of zinc finger nucleases (ZFNs) and piggyBac technology in

105 ering sequence-specific nucleases, including zinc finger nucleases (ZFNs) and TAL effector nucleases

106 This method is applicable with both zinc finger nucleases (ZFNs) and Tale nucleases (TALENs)

107 ablished a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator

108 Zinc finger nucleases (ZFNs) and transcription activator

109 Zinc finger nucleases (ZFNs) are engineered restriction

110 Zinc finger nucleases (ZFNs) are hybrid proteins that ha

111 Zinc finger nucleases (ZFNs) are powerful tools for gene

112 Here it is reported that designed zinc finger nucleases (ZFNs) can drive site-directed DNA

113 ed double-strand break induced by engineered zinc finger nucleases (ZFNs) can stimulate integration o

114 Zinc finger nucleases (ZFNs) facilitate tailor-made geno

115 We describe the use of designed zinc finger nucleases (ZFNs) for efficient transgenesis

116 on of genomic double-strand breaks (DSBs) by zinc finger nucleases (ZFNs) has been deployed for gene

117 The development of zinc finger nucleases (ZFNs) has permitted efficient gen

118 Engineered zinc finger nucleases (ZFNs) induce DNA double-strand br

119 A pair of zinc finger nucleases (ZFNs) or transcription activator-

120 Zinc finger nucleases (ZFNs) show promise in improving t

121 The first truly targetable reagents were the zinc finger nucleases (ZFNs) showing that arbitrary DNA

122 off-target site for the extensively studied zinc finger nucleases (ZFNs) targeting C-C chemokine rec

123 stability in mammalian systems, we developed zinc finger nucleases (ZFNs) that recognize and cleave C

124 by the transient expression of CCR5-targeted zinc finger nucleases (ZFNs) to generate CCR5-negative c

125 5 (CCR5) locus in pigtailed macaque HSPCs by zinc finger nucleases (ZFNs) was feasible.

126 ds for identifying the off-target effects of zinc finger nucleases (ZFNs) were described-one using an

127 Novel zinc finger nucleases (ZFNs) were designed to target the

128 Custom-designed zinc finger nucleases (ZFNs), proteins designed to cut a

129 Zinc finger nucleases (ZFNs), which are nucleases conjug

130 dopsis genes through regulated expression of zinc finger nucleases (ZFNs)-enzymes engineered to creat

131 Here we report on the use of zinc finger nucleases (ZFNs)-hybrid synthetic restrictio

132 nduction of site-specific genomic DSBs using zinc finger nucleases (ZFNs).

133 Zinc-finger nucleases (ZFNs) allow targeted gene inactiv

134 relies on cleavage of the target by designed zinc-finger nucleases (ZFNs) and production of a linear

135 While the specificity of zinc-finger nucleases (ZFNs) and RNA-guided endonuclease

136 Zinc-finger nucleases (ZFNs) and TAL effector nucleases

137 This study optimized zinc-finger nucleases (ZFNs) and transcription activator

138 of engineered site-directed nucleases, like zinc-finger nucleases (ZFNs) and transcription activator

139 Zinc-finger nucleases (ZFNs) and transcription activator

140 Zinc-finger nucleases (ZFNs) are hybrids between a nonsp

141 Zinc-finger nucleases (ZFNs) are important tools for gen

142 Zinc-finger nucleases (ZFNs) are powerful tools for edit

143 Engineered zinc-finger nucleases (ZFNs) are promising tools for gen

144 Zinc-finger nucleases (ZFNs) are targetable DNA cleavage

145 Zinc-finger nucleases (ZFNs) are versatile reagents that

146 Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome

147 ly, we and others have shown that customized zinc-finger nucleases (ZFNs) can introduce targeted fram

148 Using zinc-finger nucleases (ZFNs) designed to flank the sickl

149 By using engineered zinc-finger nucleases (ZFNs) designed to target an integ

150 Zinc-finger nucleases (ZFNs) drive efficient genome edit

151 Zinc-finger nucleases (ZFNs) drive highly efficient geno

152 Engineered zinc-finger nucleases (ZFNs) enable targeted genome modi

153 The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is h

154 We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline di

155 Engineered zinc-finger nucleases (ZFNs) form the basis of a broadly

156 Zinc-finger nucleases (ZFNs) have been used for genome e

157 Zinc-finger nucleases (ZFNs) have emerged as powerful to

158 Zinc-finger nucleases (ZFNs) have enabled highly efficie

159 Recently, microinjection of engineered zinc-finger nucleases (ZFNs) in embryos was used to gene

160 specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs.

161 olution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-strand

162 d genetic engineering of this parasite using zinc-finger nucleases (ZFNs) that produce a double-stran

163 We designed zinc-finger nucleases (ZFNs) that promoted the disruptio

164 Using zinc-finger nucleases (ZFNs) to cleave the chromosomal t

165 -resistant genotype de novo using engineered zinc-finger nucleases (ZFNs) to disrupt endogenous CCR5.

166 We tested the feasibility of using zinc-finger nucleases (ZFNs) to knock out a gene directl

167 Genome editing driven by zinc-finger nucleases (ZFNs) yields high gene-modificati

168 gene-targeting approach in the monarch using zinc-finger nucleases (ZFNs), engineered nucleases that

169 s strains (including wild-type CC-125) using zinc-finger nucleases (ZFNs), genetically encoded CRISPR

170 Designer nucleases, like zinc-finger nucleases (ZFNs), represent valuable tools f

171 uding homing endonucleases or meganucleases, zinc-finger nucleases (ZFNs), TAL effector nucleases (TA

172 From the pioneering work using zinc-finger nucleases (ZFNs), to the advent of the versa

173 Targeted nucleases, including zinc-finger nucleases (ZFNs), transcription activator-li

174 ree classes of targetable cleavage reagents: zinc-finger nucleases (ZFNs), transcription activator-li

175 Using engineered zinc-finger nucleases (ZFNs), we disrupted CCR5 in human

176 rgeting in mice using embryonic injection of zinc-finger nucleases (ZFNs), which generate site-specif

177 To stably modify this organism, we used zinc-finger nucleases (ZFNs), which take advantage of ho

178 Zinc-finger nucleases (ZFNs)-enzymes engineered to creat

179 address this problem by genome editing with zinc-finger nucleases (ZFNs).

180 with similar efficiency and precision as do zinc-finger nucleases (ZFNs).

181 endogenous soybean (Glycine max) genes using zinc-finger nucleases (ZFNs).

182 embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs).

183 nockout in mammalian cells, using engineered zinc-finger nucleases (ZFNs).

184 t a specific site have been introduced using zinc-finger nucleases (ZFNs).

185 of a targeted-mutagenesis approach based on zinc-finger nucleases (ZFNs).

186 ctivator-like effector nucleases (TALENs) or zinc-finger nucleases (ZFNs).