ライフサイエンスコーパス: zonae

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1 Mouse zonae are composed of three glycoproteins (ZP1, ZP2, and

2 , the matrix was more loosely organized than zonae around normal oocytes.

3 Mouse sperm bind to zonae comprised solely of mouse ZP2 and ZP3.

4 glycoproteins (ZP1, ZP2, and ZP3), while rat zonae contain four (ZP1, ZP2, ZP3, and ZP4/ZPB).

5 -glycosylation sites are occupied in the rat zonae glycoproteins (three of three for ZP1, six or seve

6 ction of the total rat proteome to which the zonae glycoproteins contribute) demonstrating that the r

7 teins contribute) demonstrating that the rat zonae glycoproteins share a high degree of conservation

8 t, we show that rat sperm also bind to these zonae, indicating that ZP2 and ZP3 contain a "minimum st

9 Zonae matrices contain three (mouse) or four (human) gly

10 rrier-associated protein and MMP-9 substrate zonae occludens-1 was degraded after ischemia, but this

11 recognition, and human sperm do not bind to zonae pellucidae (ZP1-3) surrounding mouse eggs.

12 n was defined by mass spectrometry of native zonae pellucidae and lies N-terminal to a proprotein con

13 ultant human ZP3 rescue females had chimeric zonae pellucidae composed of mouse ZP1, mouse ZP2 and hu

14 The recent observation that human zonae pellucidae contain a fourth protein raises the pos

15 Human sperm bound only to zonae pellucidae containing human ZP2, either alone or c

16 ologs, mouse lines with human-mouse chimeric zonae pellucidae have been established.

17 ansgenic mice expressing human ZP4 that form zonae pellucidae in the absence of mouse or human ZP2.

18 In this report, native zonae pellucidae were isolated and structural features o

19 Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct subs

20 tiate into oocyte-like cells that synthesize zonae pellucidae, organize surrounding somatic cells int

21 , ZP2, ZP3, and ZP4/ZPB were detected in rat zonae pellucidae.

22 nsport within the oviducts and in binding to zonae pellucidae.

23 and human sperm binding to their respective zonae pellucidae.

24 The primary sequences of the rat zonae proteins have been deduced from cDNA.

25 wed selective increases in the widths of the zonae reticularis and glomerulosa (p < 0.001).

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