ライフサイエンスコーパス: zygote

1 igh efficiency (0.62%-5.13% of microinjected zygotes).

2 ity gradients and polarity in the C. elegans zygote.

3 a failure to form astral microtubules in the zygote.

4 maintain cortical polarity in the C. elegans zygote.

5 al requirement in the Caenorhabditis elegans zygote.

6 petuated from oogenesis or reacquired by the zygote.

7 ferent cell types have emerged from the same zygote.

8 ted role for rRNA transcription in the mouse zygote.

9 arallels with polarization of the C. elegans zygote.

10 ired for two haploid gamete nuclei to form a zygote.

11 oth genetic material and centriole(s) to the zygote.

12 ntee a normal bipolar mitotic spindle in the zygote.

13 r centromere specification in the developing zygote.

14 llows the two gametes to fuse and create the zygote.

15 egulates the phospholipid composition in the zygote.

16 ix and fuses with the egg cell, generating a zygote.

17 ptional programs of generative cells and the zygote.

18 ne to predict developmental potential of the zygote.

19 fusion, and before the first cleavage of the zygote.

20 l-cell fusion leading to the production of a zygote.

21 tions in CTF7 exhibit mitotic defects in the zygote.

22 gans originates in the polarized single-cell zygote.

23 rm coexist as separate haploid nuclei in the zygote.

24 contractility in the Caenorhabditis elegans zygote.

25 on of centrosomes after fertilization in the zygote.

26 marker of the developmental potential of the zygote.

27 ognize each other and fuse to form a diploid zygote.

28 fferent cell lineages during cleavage of the zygote.

29 d gametic cells of each mating type and from zygotes.

30 lowed by chloroplast DNA hypermethylation in zygotes.

31 abrogated paternal DNA hydroxymethylation in zygotes.

32 drial-encoded mCherry was microinjected into zygotes.

33 duction of heteroplasmy shift in oocytes and zygotes.

34 e transposase, into the cytoplasm of porcine zygotes.

35 t3-depleted and/or DNA replication-inhibited zygotes.

36 ndle and ensures symmetric division of mouse zygotes.

37 favorable for the survival of the resulting zygotes.

38 xtra centrosomes are detected in a subset of zygotes.

39 injection of CRISPR/Cas9 reagents into mouse zygotes.

40 zation overlaps with the array in polarizing zygotes.

41 s completion and formation of normal diploid zygotes.

42 n and RNA encoding piggyBac transposase into zygotes.

43 of Tet3 in the conversion of 5mC to 5hmC in zygotes.

44 onitor the paternal DNA methylation state in zygotes.

45 9 DNA/mRNA and single-guide RNA (sgRNA) into zygotes.

46 uently in kar2 and kar8, than in kar5 mutant zygotes.

47 godeoxynucleotide (ssODN) complex into mouse zygotes.

48 ell-based system and microinjection of mouse zygotes.

49 ernal and maternal nuclei within single-cell zygotes.

50 hort stretches of DNA sequences into porcine zygotes.

51 bonucleoprotein (ctRNP) complexes into mouse zygotes.

52 single-stranded repair template into porcine zygotes.

53 and display distinct compartmentalization in zygotes.

54 after fertilization in pig and rhesus monkey zygotes.

55 re not well tolerated by normally fertilized zygotes.

56 e the origin of the first centrosomes in the zygote [2-4].

57 (75%); however, a significant portion of ST zygotes (52%) showed abnormal fertilization as determine

58 red indirectly for nuclear fusion; sey1Delta zygotes accumulate ER at the zone of cell fusion, causin

59 s provide important insights into gamete and zygote activity in plants, and our RNA-seq transcriptome

60 Simultaneous gene editing in zygotes affords an efficient approach for producing mice

61 Mild hypothermic (30 degrees C) culture of zygotes after microinjection increased HDR efficiency fo

62 This misregulation of 5hmec in zygotes also affected the level of NANOG expression in t

63 the first asymmetrical cell division of the zygote, alter planes and number of cell divisions in ear

64 cript abundance of TET3 was high only at the zygote and 2-cell stage.

65 ace during a window(s) of opportunity in the zygote and early embryo; (ii) there is no statistical va

66 o occur gradually, with the initial steps of zygote and embryo development being primarily maternally

67 (S(ua)) fuses with the egg cell, forming the zygote and embryo.

68 ability to directly modify the genome in the zygote and generate edited animals is highly desirable.

69 fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic bi

70 amete-derived methylation to maintain in the zygote and preimplantation embryo at a time when much of

71 l cells of the female gametophyte and in the zygote and proliferating endosperm of the Arabidopsis (A

72 we characterize the dynamics of MEX-5 in the zygote and propose a novel reaction/diffusion model to e

73 ametic functions, and their contributions to zygote and seed development.

74 One sperm fuses with the egg to form the zygote and the other fuses with the central cell to form

75 he surface of P. falciparum macrogametes and zygotes and effectively prevented parasites from develop

76 gene encoding OCT4 (POU5F1) in diploid human zygotes and found that blastocyst development was compro

77 th high SPTRX3 produced fewer two-pronuclear zygotes and had a reduced pregnancy rate (19.2% pregnant

78 , is present in the nucleus and cytoplasm of zygotes and has been associated with protecting the fema

79 cinity of sperm mitochondrial sheaths in the zygotes and increased in the embryos treated with protea

80 ecific DNase I hypersensitive sites in mouse zygotes and morula embryos, and investigate the epigenet

81 berghei PIMMS2 is specifically expressed in zygotes and ookinetes and is localized on the ookinete s

82 Pfs25, an antigen expressed on malaria zygotes and ookinetes, is a leading transmission blockin

83 of Plasmodium falciparum including gametes, zygotes and ookinetes, is one of the primary targets for

84 sure of DNA methylation has been observed in zygotes and primordial germ cells, the responsible enzym

85 ocalization pattern of Mcp5 in fission yeast zygotes and show by perturbation of phosphatidylinositol

86 ion is linked to asymmetric cell division in zygotes and stomatal lineages, which require integrated

87 Microinjection of the vectors into zygotes and transfer of the embryos to recipient animals

88 tact and enucleated metaphase and interphase zygotes and two-cell embryos.

89 anscriptomic homeostasis in fertilized eggs, zygotes and two-cell embryos.

90 ed yeast I-SceI homing endonuclease in maize zygotes and/or developing embryos.

91 the onset of ring assembly in the C. elegans zygote, and provide a framework for determining emergent

92 d RNA-seq transcriptome profiles of gametes, zygotes, and apical and basal daughter cells.

93 ned the dynamics of H3K9me2 changes in mouse zygotes, and investigated the regulatory mechanisms.

94 sites support multi-cell adhesions, triploid zygotes are rare, indicating a fusion-triggered block to

95 Zygote arrest (Zar) proteins are crucial for early embry

96 the first report of a molecular function of zygote arrest proteins.

97 Using the Caenorhabditis elegans zygote as a model, we find that the localization and act

98 In Plasmodium, meiosis occurs in diploid zygotes as they develop into haploid motile ookinetes in

99 We believe that pronuclear transfer between zygotes, as well as the recently described metaphase II

100 okinesis as well as for the establishment of zygote asymmetry during embryogenesis in Caenorhabditis

101 of metazoa to reset centriole number in the zygote at fertilization.

102 ting in a stable protein gradient across the zygote at steady state.

103 nce fusion occurs, in many organisms the new zygote becomes incapable of further membrane fusion reac

104 despread active and passive demethylation in zygotes before the first mitotic division.

105 Among normally fertilized ST zygotes, blastocyst development (62%) and embryonic stem

106 abnormal fertilizations leading to triploid zygotes, but also normally fertilized zygotes can sponta

107 he anterior-posterior axis of the C. elegans zygote by inducing the formation of complementary cortic

108 The formation of a zygote by the fusion of egg and sperm involves the two g

109 or destroying mitochondria delivered to the zygote by the sperm [4-13].

110 oops and domains have been detected in mouse zygotes by single-nucleus Hi-C (snHi-C), but not bulk Hi

111 sponsible for the appearance of 5hmec in the zygotes by TET3.

112 Diminution of CDC6 level in mouse zygotes by two different methods results in accelerated

113 d macrogametes for the development of motile zygotes, called ookinetes, which invade and transverse t

114 oth TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs.

115 Fertilized mouse zygotes can reprogram somatic cells to a pluripotent sta

116 leavage embryos, but we also discovered that zygotes can spontaneously segregate entire parental geno

117 iploid zygotes, but also normally fertilized zygotes can spontaneously segregate entire parental geno

118 ignificant number of normal fertilized eggs (zygotes) can be obtained for reprogramming studies.

119 ising from DNA demethylation, which prevents zygotes carrying unrepaired lesions from entering mitosi

120 e the transfer of nuclear DNA from an egg or zygote containing defective mitochondria to a correspond

121 designated as CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), enables highly efficient and high-t

122 r envelope breakdown (NEBD), we find that in zygotes cyclin A2 remains stable for a significant perio

123 ctile dynamics in the Caenorhabditis elegans zygote cytokinetic ring.

124 Division of the Arabidopsis zygote defines two fundamentally different developmental

125 chiral counter-rotating cortical flow in the zygote, depend on myosin activity, and can be altered th

126 While PBNT zygotes developed to blastocysts less frequently (42%) t

127 el plant system, we determined the timing of zygote development and generated RNA-seq transcriptome p

128 Global demethylation is required for early zygote development to establish stem cell pluripotency,

129 ought that transcription is not required for zygote development.

130 ELL1-like transcription factor essential for zygote development.

131 mmed signal transduction events critical for zygote development.

132 sphorylation pathways involved in sexual and zygote differentiation.

133 Like YDA, GRD promotes zygote elongation and basal cell fates.

134 n the egg cell after cellularization, in the zygote/embryo immediately after fertilization and in the

135 The aborted seeds contained endosperm but no zygote/embryo, reminiscent of autonomous endosperm devel

136 lly along the anterior-posterior axis of the zygote, ensuring the daughter cells a unique inheritance

137 the transcriptomes of viable and non-viable zygotes, especially in expression of genes important for

138 we report that in the Caenorhabditis elegans zygote, feedback between active RhoA and myosin induces

139 criptomes of time-staged isogenic and hybrid zygotes following fertilization.

140 h gene futile cycle (fue) is required in the zygote for male pronucleus-centrosome attachment and fem

141 reprogramming of the epigenome to prime the zygote for totipotency.

142 ocytes are enriched in proteins required for zygote formation and functions after fertilization; prot

143 In the sexual stages, zygote formation and initial ookinete differentiation pr

144 oss of activity were closely associated with zygote formation in mating-cell pairs, supporting a role

145 greatly in cells undergoing conjugation for zygote formation, and the LD fraction from these cells c

146 ovements of the nucleus are essential during zygote formation, cell migrations, and differentiation o

147 To accommodate the large cells following zygote formation, early blastomeres employ modified cell

148 otic Oct4 suggest that it is dispensable for zygote formation, early cleavage and activation of Nanog

149 for genetic diversity through the control of zygote formation, recombination, and gametogenesis.

150 for normal nuclear membrane breakdown after zygote formation.

151 gy in livestock by enabling gene knockout in zygotes from any chosen mating.

152 By examining a large set of zygotes from in vitro fertilization (IVF), we find that

153 d 627 genes that are specifically induced in zygotes; furthermore, these sex-related gene sets were e

154 By microinjecting tru-RGN RNAs into zygotes, FVII KO mice were generated with higher efficie

155 m tsRNA fractions from HFD males into normal zygotes generated metabolic disorders in the F1 offsprin

156 e RNAs (sgRNAs) targeting Tet1 and Tet2 into zygotes generated mice with biallelic mutations in both

157 The initially symmetric fucoid zygote generates a developmental axis that determines no

158 ation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-der

159 CRISPR-Cas9 mediated zygote genome editing enables high efficient production

160 When combined with efficient zygote genome editing technologies, xenogeneic human plu

161 During polarization of the C. elegans zygote, germline RNA granules, called P granules, assemb

162 ll embryonic stem cell lines derived from ST zygotes had normal euploid karyotypes and contained excl

163 Genetic mosaicism arises when a zygote harbors two or more distinct genotypes, typically

164 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and

165 ate paternally derived mitochondria from the zygote have been sought, the developmental stage at whic

166 were identified from 50 ng of Xenopus laevis zygote homogenate, which is comparable with an offline s

167 Inner layer assembly did not make the zygote impermeable as previously proposed.

168 their roles in the symmetric division of the zygote in early mouse development.

169 and redundant pathway that can polarize the zygote in the absence of ECT-2-dependent cortical flows.

170 important for the self-renewal of fertilized zygotes in Caenorhabditis elegans and neuroblasts in Dro

171 RNA) and protein are highly localized in the zygote, in a largely overlapping pattern at nuclear memb

172 of fusion-essential proteins and renders the zygote incapable of fusion.

173 ces in gene expression to be resolved in the zygote, including pathways affecting chromatin configura

174 iptomic changes were observed in unicellular zygotes, including upregulation of S-phase genes, a char

175 zation resulted in the reduction of 5hmec in zygotes indicating that TET3 is a key molecule for 5hmec

176 ion at meiosis II, thereby ensuring that the zygote inherits the appropriate complement of chromosome

177 eauty transposase were generated by standard zygote injection also on an albino background.

178 s during CRISPR/Cas9-mediated mutagenesis by zygote injection in mice.

179 i-allelic null mutations in the Tyr locus by zygote injection of two single-guide and Cas9 RNAs.

180 new route for genome engineering in pigs via zygote injection should greatly enhance applications in

181 current approaches with this method require zygote injection, making it difficult to assess the adul

182 ch 100% which implies that genome editing by zygote injections can facilitate the one-step generation

183 iates following the transverse division of a zygote into an apical, proembryo cell and a basal cell t

184 ssential for the development of a totipotent zygote into an embryo with defined cell lineages.

185 This appearance of 5hmec in zygote is important for the expression of NANOG in the b

186 Polarization of the C. elegans zygote is initiated by ECT-2-dependent cortical flows, w

187 We propose that polarity in the C. elegans zygote is initiated by redundant ECT-2- and PAR-2-depend

188 e early embryogenesis, and the genome of the zygote is only switched on later.

189 ition from differentiated cell to totipotent zygote is unknown.

190 t, although the conversion of 5mC to 5hmC in zygotes is an enzyme-catalyzed process, loss of 5hmC dur

191 entrosome detachment phenotype in C. elegans zygotes is described.

192 rapid, fusion-triggered cleavage of HAP2 in zygotes is distinct from degradation occurring during co

193 5hmC associated with the paternal genome in zygotes is gradually lost during preimplantation develop

194 The cleavage furrow in Xenopus zygotes is positioned by two large microtubule asters th

195 zation, as the cytoplasm of pronuclear-stage zygotes is reportedly inactive.

196 efficient, as treating an average of only 50 zygotes is sufficient to produce a correctly targeted al

197 The division of plant zygotes is typically asymmetric, generating daughter cel

198 tensively used to manipulate the germline in zygotes, its application in postnatal gene editing remai

199 e dynamics that drag the nucleus through the zygote; known as horsetail movement.

200 ecific knockdown of H3.3 in fertilized mouse zygotes leads to developmental arrest at the morula stag

201 ng TALEN plasmids into rhesus and cynomolgus zygotes leads to effective gene editing of MECP2 with no

202 how that in wild-type Caenorhabditis elegans zygotes, maternal pericentriolar proteins are not recrui

203 ter offspring stress responsivity and, using zygote microinjection of the nine specific miRs, demonst

204 n induce targeted CpG methylation in mice by zygote microinjection, thereby demonstrating its potenti

205 similar degree of mosaicism when compared to zygote microinjection.

206 n transgenic technology such as isolation of zygotes, microinjection of NAs into them, and their subs

207 nts suggest that epigenetic silencing in the zygote might act predominantly through female-dependent

208 We propose that dimerization of GEX1 in the zygote might be an upstream step in a signaling cascade

209 Human zygotes might therefore be useful for producing patient-

210 In contrast to human zygotes, mouse zygotes reprogrammed the somatic cell gen

211 gotes results in minimal carry-over of donor zygote mtDNA and is compatible with onward development t

212 st as animals develop from a single cell-the zygote-multicellular rosettes of S. rosetta develop from

213 te that the global chromatin organization of zygote nuclei is fundamentally different from that of ot

214 k2 accumulation in both sperm pronucleus and zygote nucleus in vivo.

215 ively expressed in the germ lineages and the zygote of maize (Zea mays).

216 TALEN-Agouti mRNAs injected into zygotes of brown FvB x C57BL/6 hybrid mice generated com

217 rotubule asters during mitosis in C. elegans zygotes or HeLa cells, respectively.

218 tion, grd;wox8;wox9 triple mutants arrest as zygotes or one-cell embryos lacking apparent polarity.

219 ed methods are not applicable to oocytes and zygotes owing to a paucity of material.

220 s of asymmetric divisions, starting from the zygote P0, each producing a transcriptionally repressed

221 stoichiometry during Caenorhabditis elegans zygote polarization, which takes place in less than 20 m

222 t all healthy cells that arise from the same zygote possess the same genomic content, with a few know

223 t these are newly transcribed in the A. suum zygote prior to pronuclear fusion.

224 It is present in murine zygotes prior to the maternal to the zygotic transition

225 arks can thus serve as an early biomarker of zygote quality in mouse model.

226 important step in understanding the basis of zygote quality.

227 ortening the half-life of Cas9 in fertilized zygotes reduces mosaic mutations and increases its abili

228 Most Setd1b(Gdf9) cKO zygotes remained in the pronuclear stage and displayed p

229 In contrast to human zygotes, mouse zygotes reprogrammed the somatic cell genome to a plurip

230 erior polarity in the Caenorhabditis elegans zygote requires two different processes: mechanical acti

231 The first cell of an animal (zygote) requires centrosomes that are assembled from pat

232 on of siRNA specific to PHLDA2 into one-cell zygotes resulted in a substantial increase in blastocyst

233 ion into Tyr heterozygous (B6CBAF1/JxFVB/NJ) zygotes resulted in the generation of numerous albinos a

234 clease and donor DNA microinjection into rat zygotes results in efficient and reproducible targeted d

235 ronuclei between abnormally fertilized human zygotes results in minimal carry-over of donor zygote mt

236 Electron tomography (ET) of zygotes revealed that mutations in these proteins block

237 cross an approximately 30-fold difference in zygote size.

238 as9 system in metaphase II (MII) oocytes and zygote stage embryos.

239 al arrest at various stages ranging from the zygote stage to the globular stage.

240 , is required for mouse development past the zygote stage.

241 mally invasive mechanical measurement at the zygote stage.

242 for injecting PEG-coated SHG nanoprobes into zygote-stage zebrafish embryos, and in vivo imaging of S

243 ilization experiments suggest that the maize zygote starts cell wall deposition within 30 seconds aft

244 s unaffected by the deletion of TDG from the zygote, suggesting the existence of other demethylation

245 vated levels of Amhr2 in two- and eight-cell zygotes, suggesting ectopic Tspo silencing before the mo

246 coding an oleosin-like protein (oleolike) in zygotes-tetrads and a transcript encoding oleosin in veg

247 tes and have lower methylation values in the zygote than in sperm.

248 lly activates mRNA species in the developing zygote that in macrogametes remain repressed via their 3

249 spark profiles revealed that parthenotes and zygotes that developed into blastocysts released more zi

250 e, we show that in advanced pronuclear-stage zygotes the paternal pronucleus contains substantial amo

251 In the zygote, the centrioles recruit pericentriolar proteins f

252 One sperm fuses with the egg to generate a zygote, the other with the central cell to produce endos

253 etric division of the Caenorhabditis elegans zygote, the PAR proteins orchestrate the segregation of

254 ng the asymmetric division of the C. elegans zygote, the RNA-binding protein MEX-5 forms an anterior-

255 delivery of targeting components directly to zygotes, these strategies are quite inefficient.

256 thylation maps in mouse gametes and from the zygote through post-implantation.

257 cell divisions to expand from a single-cell zygote to a full organism.

258 eaching of those religions that consider the zygote to be a human person with an immortal soul.

259 olites in whole zebrafish from the period of zygote to free-swimming larvae 6 days postfertilization

260 tein complex can be microinjected into mouse zygotes to edit endogenous sites with the 5'-YG-3' PAM,

261 production of mature gametes and fertilized zygotes to favorable nutritional conditions improves rep

262 nucleases and donor DNA microinjection into zygotes to generate HDR-modified rats with large new seq

263 s9 DNA/RNA and single guide RNA (sgRNA) into zygotes to generate modified animals in one step.

264 irected repair template into NOD single-cell zygotes to introduce the Ptpn22(R619W) mutation to its e

265 lease (ZFN)-encoding mRNA or DNA into bovine zygotes to verify cleavage activity at their target site

266 effector nucleases (TALEN) mRNAs into mouse zygotes transferred into foster mothers efficiently gene

267 is uniquely reorganized during the oocyte-to-zygote transition in mice and is distinct in paternal an

268 Analyzing the oocyte-to-zygote transition in the worm, Cheng et al. and Parry et

269 of the paternal genome during the oocyte-to-zygote transition.

270 nce of H3K9me2 in the male pronucleus of the zygote treated with cycloheximide.

271 mere lengths progressively increase from the zygote, two-cell to four-cell embryo.

272 at representative stages of PED, including: zygote, two-cell, four-cell, eight-cell, 16-cell, morula

273 ight-dependent germination, during which the zygote undergoes meiosis that gives rise to four vegetat

274 the germination of the alga, under which the zygote undergoes meiosis, in a positive manner, similar

275 taged preimplantation human embryos from the zygote until the blastocyst.

276 The formation of a zygote via the fusion of an egg and sperm cell and its s

277 Here we report that zygote viscoelastic properties can predict blastocyst fo

278 CRISPR/Cas9 genomic editing in murine zygotes was used to generate knockin mice with a catalyt

279 Using these zygotes, we found that when the zygotic genome was repla

280 -interfering-RNA-mediated knockdown in mouse zygotes, we identified Elp3 (also called KAT9), a compon

281 ed donor oligonucleotides (ssODN) into mouse zygotes, we introduced defined genomic modifications in

282 mic injections of TALEN mRNAs into livestock zygotes were capable of inducing gene KO in up to 75% of

283 nctions as a redox signal to activate nodal, zygotes were injected with mRNA encoding either mitochon

284 tudies involving abnormally fertilized human zygotes were not well tolerated by normally fertilized z

285 fully fertile hermaphrodite plant to produce zygotes when self-pollinated'--a definition that is neut

286 repair (BER) pathway, as is the case in the zygote where the paternal pronucleus undergoes active DN

287 ube (PT) fuses with the egg cell to form the zygote, whereas the second unites with the central cell

288 ernal genome is actively demethylated in the zygote while the maternal genome undergoes subsequent pa

289 ctive mitochondria to a corresponding egg or zygote with normal mitochondria.

290 after fertilization to produce a totipotent zygote with the potential to generate a new organism.

291 ver Cas9/sgRNA ribonucleoproteins into mouse zygotes with 100% efficiency for in vivo genome editing.

292 nd conditional mutant mice by coinjection of zygotes with Cas9 mRNA and different guide RNAs (sgRNAs)

293 Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be a

294 ovaries, and demonstrated that injection of zygotes with Cas9 mRNA and sgRNA is an efficient and rel

295 developmental impact of sperm miRs in early zygotes with single-cell amplification technology, ident

296 efficiency of gene editing in rhesus monkey zygotes, with no detected off-target effects at selected

297 was expressed at high levels in oocytes and zygotes, with rapidly declining levels at the two-cell s

298 ti locus induced site-directed DNA breaks in zygotes within 6 h of injection, an activity that contin

299 nes and to facilitate gene knockout in mouse zygotes without sacrificing on-target cleavage efficienc

300 ryos, and the developmental potential of the zygote, yet little is known quantitatively about the rel