ライフサイエンスコーパス: zymography

1 MP-3/4 as assessed by zymography and reverse zymography.

2 by RT-PCR, Western blot, activity assay, and zymography.

3 MMP-3 activity was not detected on zymography.

4 y quantitative reverse transcription-PCR and zymography.

5 nitude lower than what has been reported for zymography.

6 tivity of amebic proteinases was examined by zymography.

7 yzed for MMP-2 and -9 activities by SDS-PAGE zymography.

8 MMP-2 by RT-qPCR, Western blot analysis, or zymography.

9 l fluid (SF) from OA patients is analyzed by zymography.

10 and TIMP activity was determined by reverse zymography.

11 oteinase-9 (MMP-9) were evaluated by in situ zymography.

12 tors, using a fluorometric assay and gelatin zymography.

13 activity were determined by Western blot and zymography.

14 activity in OA SF was determined by gelatin zymography.

15 d media were determined by ELISA and gelatin zymography.

16 MMP-2 activity was analyzed by gelatin zymography.

17 d gelatinolytic activity detected by in situ zymography.

18 d conditioned media were analyzed by gelatin zymography.

19 ones that cannot be analyzed by traditional zymography.

20 and protease activity was assessed by casein zymography.

21 ytic activity in the limb as measured by gel zymography.

22 l protein extracts was determined by gelatin zymography.

23 nd tissue inhibitor of MMP (TIMP) by reverse zymography.

24 trains PA103 and ATCC 19660 were analyzed by zymography.

25 -2 and -9 activity was determined by gelatin zymography.

26 ea and conjunctiva was determined by in situ zymography.

27 and TIMPs were analyzed by Western blot and zymography.

28 s assessed in tissue culture supernatants by zymography.

29 stern blot analysis, colorimetric assay, and zymography.

30 conditioned media as demonstrated by gelatin zymography.

31 SA, and MMP-3 activity was assayed by casein zymography.

32 oteinase (MMP) activities were determined by zymography.

33 Collagenolytic activity was examined by zymography.

34 talloproteinase-9 activity in their lungs on zymography.

35 scription PCR, Western blotting, and gelatin zymography.

36 ed from the dispersion assay was assessed by zymography.

37 teinase (MMP)-2 and -9 was quantified by gel zymography.

38 and tear matrix metalloproteinase (MMP)-9 by zymography.

39 MMP-3 activity was assayed by casein zymography.

40 mogenic peptide assay and plasminogen-linked zymography.

41 predominantly MMP-9 (92 kDa GLSE) by gelatin zymography.

42 by immunofluorescence microscopy and gelatin zymography.

43 inolytic activity as demonstrated by in situ zymography.

44 inase (MMP)-2 and -9 was assessed by gelatin zymography.

45 li were found to display amidase activity by zymography.

46 by Western blotting and immunoprecipitation-zymography.

47 s determined by Western blotting and gelatin zymography.

48 ed MMP-2 enzymatic activity as determined by zymography.

49 be detected at a level of 0.2 ng in gelatin zymography.

50 low levels in conventional casein or gelatin zymography.

51 red on type I collagen films, as assessed by zymography.

52 y RT-PCR and secretion by ELISA, luminex, or zymography.

53 nd MMP-9 expression was confirmed by gelatin zymography.

54 transcriptase-polymerase chain reaction, and zymography.

55 nzyme-linked immunosorbent assay (ELISA) and zymography.

56 tion dependent manner as measured by gelatin zymography.

57 media were used for immunoblot analyses and zymography.

58 ccording to SDS-PAGE, shown a single band in zymography.

59 ive real-time polymerase chain reaction, and zymography.

60 Only MMP-9 activity was decreased on zymography.

61 thod was similar to the results of substrate zymography.

62 ch the results are consistent with substrate zymography.

63 eaction (qPCR), western blotting, and casein zymography.

64 eal-time PCR; activity of MMP-2/9 by gelatin zymography.

65 y of the inhibitors as observed in substrate zymography.

66 merase chain reaction, Western blotting, and zymography.

67 ression by real-time PCR, and MMP release by zymography.

68 -1 protein levels were assessed by ELISA and zymography.

69 nzyme-linked immunosorbent assay and gelatin zymography.

70 n and its MMP inhibitory activity by reverse zymography.

71 IMP-3-deficient mice was examined by in situ zymography.

72 determined by quantitative real-time PCR and zymography.

73 psin K to femtomole resolution using gelatin zymography.

74 ochemistry, quantitative RT-PCR, and in situ zymography.

75 ty levels of MMP-2 and -9 were determined by zymography.

76 by using MMP immunostaining and in situ MMP zymography.

77 termined by immunohistochemistry and gelatin zymography.

78 -like assay is comparable to that of gelatin zymography, a method widely used to detect gelatinases.

79 omes were incubated at 37 degrees C prior to zymography, an intense band of proteolytic activity deve

80 Zymography analysis showed antibody treatment decreased

81 Zymography analysis, performed in muscularis whole mount

82 48 hours to evaluate the MMP-9 production by zymography and activity assay.

83 y, behavioural tests, western blotting, MMP9 zymography and analysis of angioneurogenesis were perfor

84 ucted, and their activities were analyzed by zymography and by expressing them in both Escherichia co

85 Gelatinolytic activity of MMP-9 and MMP-2 by zymography and cellular localization by immunohistochemi

86 ity within the HL was examined using in situ zymography and confocal laser scanning microscopy after

87 alloproteinase-2 (MMP-2) activity by gelatin zymography and diminished MMP-2 transcription of a minim

88 Zymography, reverse zymography and ELISA (enzyme-linked immunosorbent assay)

89 ere collected after 24 hours of exposure for zymography and ELISA.

90 tissue homogenates and quantified by gelatin zymography and ELISA.

91 Gelatin zymography and enzyme activity assays performed on prote

92 rotease detection systems, including gelatin zymography and enzyme linked immunosorbent assay (ELISA)

93 mal fibroblasts, as measured by both gelatin zymography and enzyme-linked immunosorbent assay (P < 0.

94 crease in MMP-1 with CTGF only (indicated by zymography and enzyme-linked immunosorbent assay).

95 In addition, our gelatinase zymography and fluorescence data confirmed that the card

96 ctivator (uPA) activities were determined by zymography and fluorogenic assays.

97 ayed for atent and active MMP-2 and MMP-9 by zymography and immunochemistry.

98 Gel electrophoresis zymography and immunofluorescence co-localization sugges

99 addition, sections were studied with in situ zymography and immunohistochemistry staining for MMP-9.

100 teinase-2 activity was quantified by gelatin zymography and immunoprecipitation.

101 detected at a level of 30 pg in transferrin zymography and MMP-1 and -13 can be detected at a level

102 ltured human RPE to assess MMP-2 activity by zymography and protein expression by Western blot.

103 einases-1 (TIMP-1) by UDCA was studied using zymography and qRT-PCR.

104 serum and liver tissue MMP9 as evidenced by zymography and quantitative real-time PCR.

105 Zymography and real-time polymerase chain reaction analy

106 ng the Dimethylmethylene Blue assay, gelatin zymography and reverse gelatin zymography respectively.

107 ected to assess MMP-2 and TIMP-2 activity by zymography and reverse zymography, proteins by Western b

108 inase 1 (TIMP-1) and TIMP-3/4 as assessed by zymography and reverse zymography.

109 hage marker CD68 expression were assessed by zymography and reverse-transcription polymerase chain re

110 mRNA expression were analyzed using gelatin zymography and RT-PCR, respectively.

111 ignal, MMP activity as determined by in situ zymography and valvular inflammation by CD68 staining we

112 MMP secretion was measured by zymography and Western analysis, and expression in patie

113 In some mice, gelatin zymography and Western blot analyses were performed on p

114 membrane proteins were extracted for gelatin zymography and Western blot analyses.

115 Gelatin zymography and Western blot analysis revealed that the a

116 Gelatin zymography and Western blot data showed that only doxycy

117 Casein zymography and Western blot of m-calpain were performed

118 Incubation media were subjected to zymography and Western blot to detect activity and expre

119 ase in MMP-9 expression detected by SDS-PAGE zymography and Western blotting in tissue homogenates of

120 Limited profiling by gelatin zymography and Western blotting indicated that the abili

121 Analysis by zymography and Western blotting showed that KSHV-infecte

122 -2 and -14 levels were determined by gelatin zymography and Western immunoblot analysis.

123 changes in MMPs and TIMPs were evaluated by zymography and Western immunoblot assay.

124 a, and MMP and TIMP levels were evaluated by zymography and Western immunoblot.

125 n the tear fluid were evaluated with gelatin zymography and with an MMP-9 activity assay kit, respect

126 TIMP-2 using substrate gel electrophoresis (zymography) and Western blot analysis.

127 talloproteinase-2 (MMP2) activities (gelatin zymography), and cellular contents of MMP2, tissue inhib

128 significant knockdown of MMP-9 activity, per zymography, and a reversal of striatal rADC (p = 0.004,

129 roteinases were detected by casein substrate zymography, and a specific antibody was used to confirm

130 x-metalloproteinases (MMPs) was evaluated by zymography, and apoptosis was evaluated by TUNEL and cas

131 uantitative reverse transcriptase (qRT)-PCR, zymography, and chromatin immunoprecipitation.

132 ubstrate assays, Western blotting, cathepsin zymography, and computational analyses, we uncovered cat

133 Immunoblot analysis, zymography, and ELISA were used to demonstrate the synth

134 activity in peritoneal fluid was assayed by zymography, and expression of tissue plasminogen activat

135 lymerase chain reaction, immunoblot, in situ zymography, and functional analyses.

136 MMP-9 in tear washings was evaluated by zymography, and gelatinase activity in the cornea and co

137 phies, and characterized by SDS-PAGE, casein zymography, and immunoblotting.

138 Using immunoblotting, in situ zymography, and immunofluorescence, we found that LTP in

139 reverse transcription-PCR (RT-PCR), gelatin zymography, and immunohistochemistry assays, the express

140 y, real-time RT-PCR, ELISA, activity assays, zymography, and immunohistochemistry.

141 MMP production was measured by gelatin zymography, and MMP-13 production and activation were de

142 evaluated by immunocytochemistry (ICC), WB, zymography, and RT-PCR.

143 2 and MMP-9 gelatinase activity assessed by zymography, and specific MMP 2/9 inhibitors significantl

144 rmed by immune fluorescence, flow cytometry, zymography, and stimulation of neutrophils.

145 assay, MMP activity was measured by gelatin zymography, and TIMP activity was determined by reverse

146 P-2 and -9 gelatinolytic activity by gelatin zymography, and tissue inhibitors of metalloproteinases

147 2 protein by Western blot, MMP-2 activity by zymography, and type IV collagen accumulation by ELISA.

148 uated via Multiplex MMP antibody arrays, gel zymography, and Western blot, which in turn proteolytica

149 These data were confirmed by immunostaining, zymography, and Western blotting.

150 introduces electrophoretic transfer protein zymography as one solution to this problem.

151 Additionally, the PLENZ zymography assay reports the kinetic properties of CIP i

152 olamine) photodegradation products, and in a zymography assay, reaction of collagen IV with products

153 activities of tPA and uPA were determined by zymography assays.

154 activator (uPA) activities were assessed by zymography assays.

155 e expression and activity by gel and in situ zymography at 24 hours, 48 hours, and 1 week after treat

156 In situ zymography confirmed that SB-3CT suppressed gelatinase a

157 Gelatin zymography confirmed the expression of MMP-9 and MMP-2 i

158 d the presence of both MMP-2 and MMP-9 while zymography could only detect MMP-2.

159 Using zymography, CTGF increased the latent and active forms o

160 In situ zymography demonstrated a robust increase in MMP activit

161 Analysis of microsomes by zymography demonstrated a single band of proteolytic act

162 Gelatin zymography demonstrated activity of MMPs released into t

163 Zymography demonstrated enzyme activity in all synovium

164 Reverse fibrin zymography demonstrated free PAI-1 in cellular releasate

165 Conversely, collagen-based in situ zymography demonstrated markedly diminished collagenase

166 In situ zymography demonstrated that activated MMP-9 surrounded

167 Zymography demonstrated that unchallenged cells produced

168 Zymography demonstrated the release of four gelatinolyti

169 clin E processing assay, in combination with zymography, demonstrated that I3C, but not its natural d

170 quid extraction technique followed by casein Zymography detection.

171 Zymography detects and characterizes proteolytic enzymes

172 lastase B and alkaline protease, and gelatin zymography differentiated all four proteases.

173 s for MMP1 and MMP3, respectively) substrate zymography, digestion was increased with supernatants fr

174 Casein zymography distinguished protease IV from elastase B and

175 MMPs were assessed by zymography, DQ-gelatin degradation solution assays, and

176 Six in vitro assays were studied: zymography, elastin congo red assay, staphylolytic assay

177 loidin staining, MMP-2 activation by gelatin zymography, expression of MT1-MMP by quantitative real-t

178 ven gelatin proteolysis, measured by gelatin zymography, FITC-gelatin conversion, and DQ-gelatin degr

179 in) of serine proteases, assessed by in situ zymography, followed by serine-protease-mediated degrada

180 Using gel zymography for matrix metalloproteinase (MMP) detection

181 The usefulness of zymography for molecular weight determination and proteo

182 Purified recombinant PASP was analyzed by zymography for protease activity.

183 The developed AuNP assay was compared with zymography for qualitative detection of urinary HAase ac

184 espective inhibitors were analyzed by casein zymography, immune assays, and quantitative polymerase c

185 eated MV fraction was subjected to substrate zymography, immunoblotting, and substrate activity assay

186 o be decreased in DN, was investigated using zymography in a DN mouse model confirming the prediction

187 was activated by heregulin-beta1 as shown by zymography in both SKBr3 and MCF-7 breast cancer cell li

188 S-PAGE, and by in situ fluorogenic substrate zymography in RPE/choroid sections.

189 c gelatin and peptide substrates, by gelatin zymography in SDS-PAGE, and by in situ fluorogenic subst

190 MMP activity, detected by in situ zymography, increased in response to injury and was maxi

191 Gelatin zymography indicated increased secretion of active matri

192 Gelatin zymography indicated that matrix metalloproteinase 2 (MM

193 Casein zymography indicated the presence of two elastase-like a

194 Here, we used casein zymography, inhibitor profiling, affinity pull-down, and

195 In situ zymography investigations on skin sections from human ka

196 CD-1 mice, we show that MMP-9 expression by zymography is increased in the injured striatum compared

197 examined by immunohistochemistry and in situ zymography (ISZ), respectively.

198 In situ zymography localized elastolysis to medial SMCs.

199 Zymography measured the relative activity levels of MMP-

200 We have developed a novel in situ zymography method that uses a synthetic substrate conjug

201 On zymography, MMP-1 and -2 did not change.

202 d -9 expression was determined using gelatin zymography, MMP-1 by western blotting and ELISA and tiss

203 Zymography of angioreactors from MMP-deficient and contr

204 Western blot analysis and gelatin zymography of aortic extracts revealed that MMP-14 defic

205 Zymography of extracellular protease activity shed into

206 Gelatin zymography of extracts from the stria vascularis confirm

207 zymography of whole eye extracts and in situ zymography of retinal tumors showed strong gelatinase ex

208 In situ zymography of the caudal forebrain revealed that testost

209 microg/ml) to growth-arrested HMC for 72 h, zymography of the conditioned medium established that on

210 In situ zymography of the proximal jejunum reveals increased gel

211 Gel zymography of whole eye extracts and in situ zymography

212 Western blots), and gelatinolytic activity (zymography) of MMP-2 (2-fold), while not affecting TIMP-

213 ties of MMP-2/9 were corroborated by in situ zymography on frozen tissue sections.

214 -type-specific markers with MMP9 and gelatin zymography on the isolated cell populations, we identifi

215 By zymography, OPN and tumor necrosis factor (TNF)-alpha pr

216 extent of MMP activity was determined by gel zymography or enzyme-linked immunosorbent assays.

217 -9 content by substrate gel electrophoresis (zymography) or Western blotting.

218 crease in MMP-9 activity detected by gelatin zymography (P<0.01).

219 Gelatin zymography performed on TAA segments confirmed the absen

220 We used substrate zymography, reverse zymography, proteinase inhibitors, and partial sequencin

221 nd TIMP-2 activity by zymography and reverse zymography, proteins by Western blot, and type IV collag

222 icrosensors were more sensitive than gelatin zymography; PSi microsensors detected the presence of bo

223 blot to determine proteoglycan degradation, zymography, radiolabeling to determine chondrocyte biosy

224 ected to immunohistochemistry, gelatinolytic zymography, real-time PCR, and Western blot analysis of

225 protein was proteolytically active in casein zymography, requiring divalent zinc.

226 ssay, gelatin zymography and reverse gelatin zymography respectively.

227 1 and MMP-9 was also determined by ELISA and zymography, respectively, in the absence or presence of

228 re determined by Matrigel assays and gelatin zymography, respectively.

229 were assayed using Western blot analysis and zymography, respectively.

230 d using (3)H-acetylated collagen and gelatin zymography, respectively.

231 sues were examined with Western blotting and zymography, respectively.

232 y mRNA analyses, immunoblotting, and gelatin zymography, respectively.

233 ntitative RT-PCR, ELISA, immunoblotting, and zymography, respectively.

234 Gelatin zymography results showed EGCG inhibits IL-6/soluble IL-

235 One- and two dimensional zymographies revealed a single polypeptide band with pro

236 served among T.b. and T.u. PABs, and reverse zymography revealed different bands, often not correlati

237 Gelatine zymography revealed different gelatinase activity patter

238 , flow cytometry for MHC class I and gelatin zymography revealed that microglial activation and expre

239 Gel zymography revealed that MMP-9 was activated and upregul

240 Casein zymography revealed that the effect of L-MIM involves a

241 At the site of degradation, in situ zymography revealed that the gelatinolytic activity, mai

242 Zymography revealed that the smaller caspase-7 product (

243 Zymography reveals that a protease activity, present in

244 real-time polymerase chain reaction, gelatin zymography, reverse transcriptase-polymerase chain react

245 Zymography, reverse zymography and ELISA (enzyme-linked

246 We used substrate zymography, reverse zymography, proteinase inhibitors, a

247 Furthermore, enzyme kinetic assays and zymography showed a higher LDH enzyme activity in LNCaP

248 Enzyme zymography showed attenuated matrix metalloproteinase-2

249 Gel zymography showed elevations in gelatinase (MMP-2 and MM

250 med by PCR) revealed sixfold more MMP-9, and zymography showed greater enzyme activity in the infecte

251 protein when compared with controls, and gel zymography showed increased MMP protein levels.

252 Gelatin zymography showed reduced levels of MMP-9 in the mt-ERK-

253 Gelatin zymography showed strong MMP activities at early time po

254 Fluorometric assay and zymography showed that adhesives with MMP inhibitors had

255 Protease activity visualized by gel zymography showed that after nerve crush, the upregulati

256 Zymography showed that EGCG blocked constitutive, IL-1be

257 In situ zymography showed that gelatinase activity was mostly co

258 In situ zymography showed that gelatinase activity was mostly co

259 Zymography showed that MMP-9 activity, which was very lo

260 hern blotting, Western blotting, and gelatin zymography showed that MMP-9 expression was significantl

261 RNase protection assays and plasminogen zymography showed that urokinase-type plasminogen activa

262 hemical and histological analyses, including zymography, simultaneous detection of perfused capillari

263 Zymography studies have also demonstrated increased acti

264 Our gelatin zymography studies on these two secreted gelatinases, pr

265 Moreover, elastin gel zymography studies showed that gel pretreatment with AlC

266 We describe a sensitive zymography technique that utilizes an automated microflu

267 Zymography techniques are routinely used to quantify pro

268 using enzyme-linked immunosorbent assays and zymography techniques that revealed MMP-1, -2, -3, and -

269 nt (BALB/c) mice before and after infection; zymography tested enzyme activity for MMP-2 and -9.

270 MMP9 activity in brains, measured by gelatin zymography, than mock-infected mice.

271 However, in contrast to zymography, the assay directly measures the enzymatic ac

272 Samples were also prepared for gel zymography to detect the presence of MMP-2, and for Nort

273 We also demonstrate the use of gelatin zymography to determine the effects of differentiation a

274 was determined by Western blot analysis and zymography, using conditioned medium and HCM cells.

275 In situ zymography validated these results.

276 this report a novel protocol for SDS gelatin zymography was established, and an increase of cathepsin

277 Gelatin zymography was performed to demonstrate enzyme activity.

278 Substrate zymography was performed to determine levels of secreted

279 e activity and biofilm maturation, autolysin zymography was performed, which revealed an altered prof

280 inase-2 expression and activation by gelatin zymography was unchanged between groups, while its endog

281 Zymography was used to assess the activity of MMP-1, -2,

282 Gelatin zymography was used to identify activated 82-kDa MMP-9.

283 Gelatin zymography was used to measure changes in the amount of

284 SDS-PAGE zymography was used to measure MMP2 and MMP9 activities.

285 In situ zymography was used to show Gel A-activity in vivo.

286 Furthermore, cathepsin K zymography was used to show that murine osteoclasts secr

287 in situ hybridization and gelatin substrate zymography) was demonstrated in EMMPRIN-enhanced tumors.

288 Using gelatin zymography, we detected constitutive MMP-2 activity in b

289 ray, quantitative PCR, Western blotting, and zymography, we found that TNF-alpha drastically increase

290 Using gelatin zymography, we showed increased pro and active MMP-2 act

291 Gelatin and casein zymographies were performed in serum-free conditioned me

292 transcription-polymerase chain reaction and zymography were performed to analyze the mRNA expression

293 Both Western blot and zymography were used to monitor the effects of noladin e

294 Real time PCR, Western blot, and zymography were used to quantify MMP mRNA, protein, and

295 MMP expression in the retina was analyzed by zymography, Western blot, and immunohistochemistry.

296 duction or activity was determined by ELISA, zymography, Western blotting and immunofluorescent stain

297 on (by Northern blot) and MMP-2 activity (by zymography), which resulted in an increase in invasivene

298 the assay sensitivity was 82.5% vs. 65% for zymography, while the specificity for both assays was 96

299 Reverse zymography with human saliva and Tenebrio molitor alpha-

300 , enzymatic activity was detected by in situ zymography within the HLs of both tested adhesives, with