ライフサイエンスコーパス: (His)6

1 with the configuration T7 promoter-TIR-regA-His6.

2 n the amino terminal region between Arg5 and His6.

3 d the trapping capacity was ~100 nmol/mg for His6.

4 n reagents phosphine-FLAG and phosphine-FLAG-His6.

5 ce remaining after removal of the C-terminal His6.

6 The 20-kDa His6-Aer2-166 PAS-F1 fragment was purified as an 800-kDa

7 Furthermore, Hyp-1 was expressed with a His6 affinity tag linked to its N terminal region using

8 ty isolation of histidine-tagged Sba1p (Sba1(His6)) after expression in yeast led to coisolation of H

9 Addition of a 6-histidine extension (HisP(His6)) allows a rapid and effective metal affinity purif

10 alysis of the assembly state showed that the His6-alpha and beta subunits released from corresponding

11 The His6-alpha-loop retained significant native structure, a

12 inely yielded approximately 20 mg of soluble His6-alpha-loop/L cell culture.

13 His6 and AHHAHHAAD AHHAHHAAD spiked in a protein digest

14 -Tyr8 cross-link is formed between N(tau) of His6 and C(beta) of Tyr8, an unprecedented type of cross

15 unction mutations, restoring efficacy to the His6 and d-Phe7 substituted peptides that had lost effic

16 constraint to enhance the beta-turn spanning His6 and D-Phe7, while the pharmacophore group in Arg8 w

17 cytoplasmic domain of MisS were purified as His6 and maltose binding protein fusion proteins, respec

18 NACK120-his6 and NACK100-his6 were dimers in solution.

19 rtant groups, namely, the side chains of the His6 and Phe8 residues.

20 Constructs with C-terminal His6 and V5 epitopes were expressed by transient transfe

21 rthermore, interaction between purified Sba1(His6) and Hsp90 in yeast extracts was inhibited by the b

22 re important for complex formation with Sba1(His6), and residues in both the N-terminal nucleotide bi

23 issociation constant between poly-His, i.e., His6, and the Fe3O4@Al2O3 MNPs was ~10(-5) M and the tra

24 tagged derivative by precipitation with anti-His6 antibodies and by Co2+ affinity chromatography.

25 n7)-Phe8 (3), Sar1-Arg2-Val3-Tyr4-cyclo(Asp5-His6-Apt7)-Phe8 (4), Sar1-Arg2-Val3-Tyr4-cyclo(Glu5-His6

26 t7)-Phe8 (4), Sar1-Arg2-Val3-Tyr4-cyclo(Glu5-His6-Apt7)-Phe8 (5), Sar1-Arg2-Val3-Tyr4-cyclo-(Cys5-His

27 ependent viral infection, as assessed in two His6 artificial receptor systems.

28 ds from the N-terminus of the protein, Arg2, His6, Asn8 and Arg10, interact with the base pairs of th

29 ents carrying nickel-binding histidine tags (his6) at their C termini were also generated.

30 ility shift assays with purified recombinant His6-AUF1 fusion protein.

31 vitro exchange studies reveal that when apo-(His6)-beta2 ((His)beta2) is mixed with beta'2, apo-(His)

32 Preparation of (His6)BglK by nickel-nitrilotriacetic acid-agarose chroma

33 nd cross-reacts with polyclonal antibody to (His6)BglK from K. pneumoniae.

34 on, consistent with the high symmetry of the His6 binding site observed crystallographically.

35 ng an in vitro assembly assay, purified Sba1(His6) bound to Hsp90 only in the presence of adenosine 5

36 in sf-9 cells infected with both p55CDC and His6-BUBR1 recombinant baculoviruses but not in the cell

37 PC components, preferentially associate with His6-BUBR1 resins, but not the control resins.

38 enhances the association between p55CDC and His6-BUBR1.

39 n reagents (phosphine-FLAG or phosphine-FLAG-His6) can be completed in approximately 1 week.

40 ecombinant baculoviruses, sf-9 cells produce His6-Cdc25C antigen with an additional slower mobility b

41 e mapping shows that His6-Prk phosphorylates His6-Cdc25C at two sites in vitro and that the major pho

42 amide gels compared with cells infected with His6-Cdc25C baculovirus alone.

43 rom sf-9 cells co-infected with His6-Prk and His6-Cdc25C baculoviruses, but not with His6-PrkK52R and

44 baculoviruses, but not with His6-PrkK52R and His6-Cdc25C baculoviruses, contains a greatly enhanced k

45 In addition, His6-Cdc25C immunoprecipitated from sf-9 cells co-infect

46 K52R, is capable of strongly phosphorylating His6-Cdc25C in vitro.

47 enhanced kinase activity that phosphorylates His6-Cdc25C in vitro.

48 We have expressed His6-Prk and His6-Cdc25C proteins using the baculoviral vector expres

49 When co-infected with His6-Prk and His6-Cdc25C recombinant baculoviruses, sf-9 cells produc

50 His6-CDPK alpha (1-328), which contained none of the CLD

51 vity of the chimeric enzyme, its addition to His6-CDPK alpha (1-398) resulted in activity that was on

52 s activated only 5-fold, but the activity of His6-CDPK alpha (1-398), which retained nearly half of t

53 (His6-eYFP), and His6-tagged collagenase G (His6-ColG), with sizes ranging from 1 to 11 nm, were use

54 The inactive SpoIVFB.Pro-sigma(K)(1-126)-His6 complex was stable during affinity purification and

55 stent with a 4:2 SpoIVFB.Pro-sigma(K)(1-126)-His6 complex.

56 de with 20-residue-long, thrombin-cleavable, His6-containing N-terminal extensions.

57 pha-MSH, gamma-MSH, and Ac-Nle4-cyclic-[Asp5,His6,D-Phe7,Arg8,Trp9,Lys10]NH2 (MT-II).

58 peptides derived from MTII, Ac-Nle-cyclo(Asp-His6-D-Phe7-Arg8-Trp-Lys)-NH2 (a pan-agonist at the mela

59 (NDP-MSH), by first defining the role of the His6-d-Phe7-Arg8-Trp9 residues in receptor activation (E

60 orbent assay (ELISA) using affinity-purified his6-DEK fusion protein.

61 ein fusion with Plasmodium knowlesi PSD (MBP-His6-Delta34PkPSD) as the enzyme.

62 the His6 epitopes did not appear to mediate His6-dependent viral infection, as assessed in two His6

63 ny genes whose expression requires DnaA, and His6-DnaA binds to the promoters of gcrA, ftsZ and podJ

64 ovel cyclic SHU9119 analogues (Ac-Nle4-[Asp5-His6-DNal(2')7-Arg8-Trp9-Lys10]-NH2) modified in positio

65 ng in a carboxyl-terminal hemagglutinin (HA)-His6 epitope.

66 The His6 epitopes did not affect the native infectivity of A

67 In addition, the His6 epitopes did not appear to mediate His6-dependent v

68 This suggested the His6 epitopes in HVR2 and HVR5 were exposed on virion su

69 yme-linked immunosorbent assay and found the His6 epitopes incorporated in HVR2 and HVR5 could bind t

70 The His6 epitopes were expressed in all modified hexon prote

71 either hemagglutinin (HA) or hexahistidine (His6) epitopes at the C-terminus.

72 in vitro assays, the degradation of purified His6-Epsilon by the His6-LonBs, ClpPBs, and ClpXBs prote

73 Small molecular mass molecules (His6) exhibit a high binding ability to all PNTs polymer

74 -tagged enhanced yellow fluorescent protein (His6-eYFP), and His6-tagged collagenase G (His6-ColG), w

75 Full-length wild-type FadR and His6-FadR bound oleoyl-CoA and myristoyl-CoA with simila

76 Complexes purified from brains of transgenic His6-FLAG-YFP-NL2 mice showed enrichment in the Gene Ont

77 We find the His6-FLAG-YFP-NL2 to be a suitable tag, with the unampli

78 The PcrA protein was overexpressed with a His6 fusion at its amino-terminal end.

79 BIG2, synthesized as a His6 fusion protein in Sf9 cells, accelerated guanosine

80 iserum raised against an overexpressed MrpG'-His6 fusion protein showed that MrpG was present in the

81 The BFP assembly complex, containing a BfpB-His6 fusion protein, was chemically cross-linked in situ

82 protein microarrays constructed with double-His6 GFP demonstrated greater detection sensitivity with

83 ution, while the dissociation rate of double-His6 GFP from Ni-NTA chips in SPR (BIAcore) was 10 times

84 en used to isolate His6-T7 bacteriophage and His6-GroEL directly from cell lysates.

85 formed cryo-electron microscopy (cryo-EM) of His6-GroEL obtained from clarified E. coli lysates.

86 activity in cytosolic extracts, and purified His6-GT78 exhibited alpha3GalT-activity toward a synthet

87 applied for detection of antibodies against His6-H5 HA in serum of vaccinated hen using serial 10-fo

88 of the recombinant His-tagged hemagglutinin (His6-H5 HA), (v) filling free space with BSA.

89 Purified HisP(his6) has a strong tendency to precipitate; 5 mM ATP and

90 HisP(his6) has been characterized with respect to substrate a

91 tracts of a McArdle cell line overexpressing His6-hemagglutinin-tagged, rat APOBEC-1 using metal-chel

92 His tag (His6), His-tagged enhanced yellow fluorescent protein (H

93 hift movements of the 2H NMR signals for the His6, His13, and His14 side chains.

94 ing of L-(-)-nicotine to histidine residues (His6, His13, and His14) in the peptide.

95 A model is proposed in which Cu(2+)-His6/His13 and Cu(2+)-His6/His14 sites alternate along t

96 oposed in which Cu(2+)-His6/His13 and Cu(2+)-His6/His14 sites alternate along the fibril axis on oppo

97 N-terminal-containing His6-tagged insulysin (His6-IDE) with proteinase K led to the initial cleavage

98 ather than the sigmoidal curve obtained with His6-IDE.

99 The protonation/deprotonation equilibrium of His6 influences the reduction potential of the protein i

100 nucleotides encoding six histidine residues (His6) into different HVRs in the Ad5 genome.

101 HisP(his6) is active as a dimer, binds ATP with a Kd value of

102 HisP(his6) is indistinguishable from underivatized HisP when

103 In contrast to the intact complex, HisP(his6) is not inhibited by vanadate but is inhibited by N

104 nding half-site capable of interacting with (His6)jRXR fusion protein was identified in the promoters

105 Finally, an antibody prepared against (His6)jRXR showed that full-length jRXR is expressed at a

106 -binding (Asp347) residue were essential for His6-LAP-A activity, as most R431 and D347 mutant His6-L

107 The E429D His6-LAP-A enzyme had Km and kcat values similar to the

108 Glu334, Arg336, and Lys262, was evaluated in His6-LAP-A fusion proteins expressed in Escherichia coli

109 Sixty-five percent of the mutant His6-LAP-A proteins were unstable or had complete or par

110 The R431K His6-LAP-A that retained the positive charge had partial

111 Km and kcat values similar to the wild-type His6-LAP-A.

112 LAP-A activity, as most R431 and D347 mutant His6-LAP-As did not hydrolyze dipeptides.

113 The activity of hexameric His6-LAP-As on Xaa-Leu and Leu-Xaa dipeptides was tested

114 of Lys354 (a catalytic residue) resulted in His6-LAP-As that cleaved dipeptides at slower rates.

115 reover, LdSec13 specifically interacted with His6-LdSec31(1-603), and LdSec31 bound the prebudding co

116 A recombinant His6-LipL41 fusion protein was expressed in Escherichia

117 degradation of purified His6-Epsilon by the His6-LonBs, ClpPBs, and ClpXBs proteases from B. subtili

118 d to estimate the secondary structure of the His6 loop, revealing an ordered folding composed of 23%

119 rther show that purified A. pleuropneumoniae His6-Lrp binds in vitro to the A. pleuropneumoniae promo

120 The strategy utilizes a dual His6-maltose binding protein (HisMBP) affinity tag that

121 uberculosis (MtUGM) was obtained from a dual His6-MBP-tagged MtUGM construct.

122 Purified His6-MM1854 occurred as a homodimer of 29-kDa subunits,

123 ng sites: the His3Asp motif (site 1) and the His6 motif (site 2).

124 titrations support the formation of a Zn(II)-His6 motif.

125 t7)-Phe8 (6), Sar1-Arg2-Val3-Tyr4-cyclo(Cys5-His6-Mpc7)-Phe8 (7), Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-His6

126 Phe8 (8), and Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-His6-Mpc7)-Phe8 (9), where Apt stands for 4-amino-trans-

127 7)-Phe8 (5), Sar1-Arg2-Val3-Tyr4-cyclo-(Cys5-His6-Mpt7)-Phe8 (6), Sar1-Arg2-Val3-Tyr4-cyclo(Cys5-His6

128 c7)-Phe8 (7), Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-His6-Mpt7)-Phe8 (8), and Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-

129 o a p-quinone methide intermediate, to which His6-N(tau) attacks to form the C-N cross-link.

130 oresis mobility shift analysis revealed that His6-NasR bound specifically to nasF leader RNA.

131 t the terminator site, and that MBP-NasR and His6-NasR proteins both caused transcription readthrough

132 sion form (MBP-NasR) and a His6-tagged form (His6-NasR).

133 Incorporation of His6-OPH in nano-OPH preserves catalytic activity and in

134 ses the immune and inflammatory responses to His6-OPH in vivo as determined by anti-OPH IgG and cytok

135 ic coupling of the hexahistidine tagged OPH (His6-OPH) and poly(ethylene glycol)-b-poly(l-glutamic ac

136 Removal of this tag from His6-p38 MAPKalpha, prior to its activation, is essentia

137 We found that addition of recombinant His6-p9 to egg extracts results in a pronounced delay of

138 ic and inhibition studies were performed for His6-PDK1(deltaPH)-catalyzed phosphorylation of PDK1-Tid

139 analogs were Sar1-Arg2-Val3-Tyr4-cyclo(Cys5-His6-Pen7)-Phe8 (3), Sar1-Arg2-Val3-Tyr4-cyclo(Asp5-His6

140 The double-His6 peptide has the potential to be a universal tag for

141 We inserted a His6 peptide into an ecotropic envelope protein (Env) by

142 region sequence with a sequence encoding the His6 peptide.

143 Plk3, but not by the kinase-defective mutant His6-Plk3(K52R), GST-p53 was recognized by an antibody s

144 recipitated from sf-9 cells co-infected with His6-Prk and His6-Cdc25C baculoviruses, but not with His

145 We have expressed His6-Prk and His6-Cdc25C proteins using the baculoviral

146 When co-infected with His6-Prk and His6-Cdc25C recombinant baculoviruses, sf-9

147 Further studies reveal that His6-Prk phosphorylates Cdc25C on serine216, a residue a

148 Moreover, phosphopeptide mapping shows that His6-Prk phosphorylates His6-Cdc25C at two sites in vitr

149 Purified recombinant His6-Prk, but not a kinase-defective mutant His6-PrkK52R

150 and His6-Cdc25C baculoviruses, but not with His6-PrkK52R and His6-Cdc25C baculoviruses, contains a g

151 His6-Prk, but not a kinase-defective mutant His6-PrkK52R, is capable of strongly phosphorylating His

152 angiotensin II (Asp1-Arg2-Val3-Tyr4-Val/IIe5-His6-Pro7-Phe8, AII), the synthesis and biological testi

153 T7 promoter on plasmid pSA1 yielded a RegA69-His6 protein that binds nickel-Sepharose and elutes with

154 bout by the structural change resulting from His6 protonation.

155 The resulting His6-PubC fusion proteins were purified by Ni-NTA affini

156 stidine6-tagged receptor-associated protein (His6-RAP) with high affinity, although variants lacking

157 siRNA to C33-A cells using oligofectamine or His6 reducible polymers, siRNA uptake was quantified by

158 gene products in which C-terminal tags (FLAG-His6) replace the native glycosylphosphatidylinositol an

159 a consequence of protonation of the surface His6 residue, is identified.

160 of Ad type 2 E3-19K tagged with a C-terminal His6 sequence in baculovirus-infected insect cells.

161 The His6 sequence is used as a ligand for the one-step affin

162 at their amino terminus, the peptide Met Gly His6 Ser Gly Leu Phe Lys Arg/, where Leu Phe Lys Arg/ is

163 opose that removing the interaction with the His6 side chain reorients the peptide within the binding

164 ude and coincides with Mn(II) binding at the His6 site as well as other sites.

165 The Zn(II)-His6 site of CP expands the known biological coordinatio

166 finity and specific binding of Mn(II) to the His6 site of human calprotectin.

167 ution, affording an unprecedented biological His6 site.

168 o SPR assay formats, with either mAb10F12 or His6-SNAP-25 coupled to the biosensor chip.

169 RegA69-His6 synthesized in E. coli S30 or wheat germ extracts d

170 fouling properties were then used to isolate His6-T7 bacteriophage and His6-GroEL directly from cell

171 s formed between 1-Cys Prx with a C-terminal His6 tag and GST pi upon incubation of the two proteins

172 engineered into an expression vector with a His6 tag at its amino terminus, and the protein was expr

173 ombinant alpha-galactosidase molecule with a His6 tag at its C-terminus was constructed by an overlap

174 The synthetic technique makes use of a His6 tag at the C terminus of the target polypeptide cha

175 1) was expressed using a vector containing a His6 tag at the N terminus.

176 Moreover, the double-His6 tag could serve simultaneously both for protein imm

177 The presence of a His6 tag enables the isolation of peptide or protein pro

178 ted into the pRSET vector and expressed as a His6 tag fusion protein in Escherichia coli.

179 de, the M. xanthus gene was expressed with a His6 tag in E. coli cells.

180 ary of mutants whose combined members have a His6 tag inserted at likely every site in the original p

181 Display of the His6 tag on the surface of Env endowed the vectors with

182 modified surfaces than a conventional single-His6 tag or two single-His6 tags at N- and C-termini.

183 attached to the carboxy end of an N-terminal His6 tag that binds to nickel (Ni2+).

184 Therefore, addition of the His6 tag to the C-terminus of RB69 RegA does not dramati

185 A His6 tag was cloned at the N terminus, along with R403Q,

186 Under denaturing conditions when the His6 tag was used, 84% of samples were purified.

187 NBD1-R (amino acids 404-830, in fusion with His6 tag) were expressed as single proteins in Escherich

188 gene, encoding a protein with an N-terminal His6 tag, was expressed in Escherichia coli.

189 9-kDa chimeric proteins, having a C-terminal His6 tag, were secreted by Saccharomyces cerevisiae, usi

190 The results show that His6 tag-assisted chemical protein synthesis is a useful

191 ion vector that also introduced a C-terminal His6 tag.

192 que except that the MutL protein carried the His6 tag.

193 60% for the Nanobody lacking the C-terminal His6 tag.

194 GFP), stable and tight binding of the double-His6 tag/Ni-NTA interaction was demonstrated by competit

195 Here we describe a double-hexahistidine (His6) tag sequence, comprising two hexahistidines separa

196 1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharom

197 on in conjunction with immobilized metal ion/His6-tag interactions to prepare highly purified recombi

198 Overexpression of GT78 with a His6-tag resulted in a 120-fold increase in GalT-activit

199 ptors and an NTA moiety to interact with the His6-tag upon cocomplexation of Ni(2+) ions.

200 terminus with either a FLAG-epitope tag or a His6-tag.

201 in Escherichia coli as catalytically active His6 tagged proteins, purified to homogeneity by affinit

202 We purified polyhistidine (His6)-tagged and native Escherichia coli MiaA tRNA preny

203 The purified hexahistidine (His6)-tagged Pseudomonas aeruginosa RhlI protein was sub

204 To test this hypothesis, a six-histidine (His6)-tagged version of MM1854 was produced.

205 and detected in immunoblots using N-terminal His6-tagged AgrD.

206 In addition, GST-NR2B and His6-tagged alpha-actinin can bind simultaneously to mon

207 mbinant sorcin bound to cardiac and skeletal HIS6-tagged alpha1 C termini immobilized on Ni2+ resin.

208 ies assembled in vivo in the presence of the His6-tagged and untagged Rpb3.

209 Purified recombinant His6-tagged apobec-1 immobilized on beads depleted >90%

210 ypothesis, R. sphaeroides mutants expressing His6-tagged bc1 complexes with mutations at three aromat

211 yellow fluorescent protein (His6-eYFP), and His6-tagged collagenase G (His6-ColG), with sizes rangin

212 Of the his6-tagged constructs derived from NACK, NACK100 was in

213 Similarly, His6-tagged densin-180 or alpha-actinin fragments associ

214 latory protein was purified as an N-terminal His6-tagged derivative and characterised both with and w

215 In the present study, His6-tagged E1 alpha2 beta2 tetramers (171 kDa) denature

216 binding protein fusion form (MBP-NasR) and a His6-tagged form (His6-NasR).

217 nterfacial properties of a carboxyl-terminal His6-tagged form of apoB20.1 (apoB20.1H).

218 spectrometry confirmed production of intact, His6-tagged forms of CYP144A1-FLV and -TRV, with EPR dem

219 We have expressed His6-tagged fragments containing the sequence of the C-t

220 6E mutants of peripherin-2 were expressed as His6-tagged full-length proteins in Madin-Darby canine k

221 atable peptide sequence to the C-terminus of His6-tagged green fluorescent protein (GFP).

222 Treatment of an N-terminal-containing His6-tagged insulysin (His6-IDE) with proteinase K led t

223 The protocol described here uses recombinant His6-tagged KDPG aldolase for the synthesis of (S)-4-hyd

224 His6-tagged M. thermautotrophicus type II IPP isomerase

225 Gene-targeted mice that contain a His6-tagged mouse c-Myc cDNA, Myc(His), inserted head to

226 We demonstrated that the His6-tagged MuLV could be produced to high titers and co

227 HPLC assay analysis of each His6-tagged mutant indicated that F337I successfully pro

228 A His6-tagged NR2B fragment associates with GST-densin or

229 In this work, the His6-tagged PduS cobalamin reductase from S. enterica wa

230 Expression of a human His6-tagged PKC-theta in Jurkat cells and purification b

231 d-biotin-tris-NTA (BTN) hybrid compound, any His6-tagged protein can be immobilized on the surface of

232 proteins in the same cell and isolating the His6-tagged protein followed by MutS immunoblotting afte

233 RNA ligands were selected by binding to His6-tagged proteins and chromatography on nickel(II) ni

234 We also characterized His6-tagged proteins enriched on-plate by the Fe3O4@Al2O

235 he pET15b constructs produced NH2-terminally His6-tagged proteins.

236 BIAcore) was 10 times slower than for single-His6-tagged proteins.

237 formation by wild-type and mutant XRCC3 with His6-tagged Rad51C upon co-expression in bacteria, nicke

238 n investigated by Raman spectroscopy using a His6-tagged recombinant protein that self-assembles in v

239 These proteins have been expressed as Met-His6-tagged recombinant proteins and purified by metal c

240 The His6-tagged Sec7 domain from ySec7p (rySec7d) synthesize

241 for the assembly of growth factors employing His6-tagged single-domain antibodies (VHH).

242 ach can be used to determine quickly whether His6-tagged species are present in a sample.

243 We capture His6-tagged TFs using nickel-nitrilotriacetic acid (Ni-N

244 His6-tagged Tsc10p was expressed in Escherichia coli and

245 ecifically recognizing Rpl17 or Rpl39, and a His6-tagged version of Rpl4, we established that all thr

246 A His6-tagged version of the SCO1135 protein product was s

247 A recombinant His6-tagged version of this protein was subsequently pro

248 e-steady-state studies were performed on the His6-tagged wild-type (WT) enzyme, several active site m

249 Dimerization was assayed by expressing His6-tagged wild-type and non-tagged deletion mutant pro

250 essed in Escherichia coli, and the resulting His6-tagged WreQ protein was purified.

251 that encode the PAS domain with and without His6 tags and expressed the PAS peptides in E. coli.

252 a conventional single-His6 tag or two single-His6 tags at N- and C-termini.

253 xpressed in Escherichia coli with N-terminal His6 tags to facilitate purification.

254 quipped lipid to bind the proteins via their His6-tags to the lipid membrane were used to bind two di

255 This deletion reduced multimerization of His6-tailed KorB protein in vitro and greatly reduced bi

256 chia coli as recombinant proteins containing His6 tails.

257 as equipped with a C-terminal hexahistidine (His6) tether to facilitate the assembly on betaCD surfac

258 His6-Thg1p purified from Escherichia coli catalyzes the

259 otein with one of eight tags: hexahistidine (His6), thioredoxin (Trx), small ubiquitin-related modifi

260 Conversely, His6 to Ala6 substitution reduced receptor activation bu

261 C-terminal end with an epitope (FLAG) and a His6 tract.

262 e wt TrfA which is largely dimeric, purified His6-TrfA(G254D/S267L) is primarily monomeric.

263 His6-TrfA(P151S) and His6-TrfA(S257F) purify as dimers,

264 His6-TrfA(P151S) and His6-TrfA(S257F) purify as dimers, and when expressed in

265 In particular, the His6-Tyr8 cross-link is formed between N(tau) of His6 an

266 eir unique cross-links between Trp1-Asn4 and His6-Tyr8.

267 s led to the identification of several VSV-G-His6 variants that were able to package high-titer viral

268 However, MBP-NACE-his6 was completely resistant to cleavage by factor Xa,

269 The limit of detection for His6 was, therefore, as low as ~400 amol.

270 se-insensitive protein variant (FixK2(C183S)-His6) was used in which Cys-183 was replaced with serine

271 The apparent Km values of PduS-His6 were 10.1+/-0.7 muM for NADH and 67.5+/-8.2 muM for

272 NACK120-his6 and NACK100-his6 were dimers in solution.

273 ng recombinant Shiga-like toxins tagged with His6 were used as the samples to further demonstrate tha

274 roximately 100-fold more active than Arg6 or His6, whereas Glu6 and (SerSerGly)2 were inactive.