ライフサイエンスコーパス: -facing

1 id-19 were seen in staff working in Covid-19-facing areas (22.6% vs. 8.6% elsewhere) (aOR 2.47 [1.99-

2 Controlling for Covid-19-facing status, risks were heterogenous across the hospit

3 Alanine mutation of the Ago2-facing Lys56 in eIF1A impairs RNAi activities in human c

4 the ADK5b epitope spans both the 5-fold axis-facing wall of the 3-fold protrusion and portions of the

5 inations of engineering structures (backward-facing steps forming d-type ribs on the porous surface o

6 ng it close the mouth, whereas the backwards-facing marginal serrations of the bivalved carapace may

7 tions compared to those from more inland bay-facing sites.

8 tein is localized at both basolateral (blood-facing) and apical (saliva-facing) membranes of salivary

9 w report that PTs express RPTPgamma on blood-facing membranes.

10 y of podosomal belts that segregate the bone-facing ruffled membrane from other membrane domains.

11 l agents, and mutations at predicted channel-facing positions modulate this effect.

12 that regard, with both clinical and consumer-facing analysis tools relying on this self-contained des

13 claim to be both domain-facing and consumer-facing have to pass the test of portability across the v

14 Pores or stomata present on CSF-facing leptomeningeal cells ensheathing blood vessels in

15 The antagonist binds to a cytoplasm-facing pocket formed by S1-S4 and the TRP helix, whereas

16 e syringe, the injectisome, with a cytoplasm-facing translocase channel.

17 ast three different conformations: cytoplasm-facing, occluded, and lumen-facing.

18 tors of VMAT2: reserpine binds the cytoplasm-facing conformation, and tetrabenazine binds the lumen-f

19 o shift the equilibrium toward the cytoplasm-facing conformation, emulating the effect of protonation

20 se structurally constrained in the cytoplasm-facing state and either apo, bound to sodium ions only,

21 The lumen- to cytoplasm-facing transition, facilitated by protonation of at leas

22 sensing, may line the channel's cytoplasmic-facing funnel surface, stabilize the channel's overall s

23 e apparent Na(+) affinity at the cytoplasmic-facing sites of the pump protein and that this effect is

24 he reduced Na(+) affinity of the cytoplasmic-facing sites precluded proper pump activation under phys

25 itor binding to the Neu5Ac site in a cytosol-facing conformation.

26 The cytosol-facing membranes of cellular organelles contain proteins

27 molecular identities between their cytosolic-facing surface membranes and their interior?

28 are libraries with a claim to be both domain-facing and consumer-facing have to pass the test of port

29 avior consisting of curved swims in downward-facing larvae but only when triggered by dimming.

30 mathematical treatment as standard (downward-facing) RDEs; it can be operated airtight and coupled to

31 ution of volcanic products between the Earth-facing lunar side and the farside is the result of a com

32 owest rates of warming evident on north-east-facing slopes.

33 water oxidation catalyst on the electrolyte-facing surface of the sealing graphite sheet, which cath

34 eems to be unaffected by operation, exterior-facing and feedstream-introduced iron was found in two f

35 mics of the protonation of the extracellular-facing gating Glu (Ex) and Cl(-) binding to the external

36 pparent Na(+) affinity for the extracellular-facing sites in the CAPOS mutant, which was related to a

37 sformed from intracellular- to extracellular-facing orientation in relation to the ion translocation

38 niques to recordings from the tablets' front-facing camera (area under the receiver operating charact

39 Two are in different inside-facing states.

40 sus (PfMATE) in the long-sought-after inward-facing state, which was obtained after crystallization i

41 conformational transitions between an inward-facing (IF) and an outward-facing (OF) conformation.

42 that acidic pH favors formation of an inward-facing (IF) conformation, whereas elevated pH (>7) and t

43 uctures of MRP1 were determined in an inward-facing (IF) or outward-facing (OF) conformation.

44 of the uracil transporter UraA in an inward-facing conformation allowed us to identify the anion-bin

45 Recently, an inward-facing conformation of LeuT was solved revealing an unex

46 g domains of the transporter adopt an inward-facing conformation with the two nucleotide-binding doma

47 The structure shows UapA in an inward-facing conformation with xanthine bound to residues in t

48 reveal an unprecedented closed and an inward-facing conformation, respectively.

49 KCC1 adopts an inward-facing conformation, with the extracellular gate occlude

50 site when the transporter rests in an inward-facing conformation.

51 a series of crystal structures in an inward-facing conformation.

52 pts a LeuT fold and is captured in an inward-facing conformation.

53 An inward-facing crystal structure and an outward-facing model of

54 ating that the transporter lies in an inward-facing open conformation.

55 ansition from an outward-facing to an inward-facing state of the transporter.

56 Can1 is promoted by transition to an inward-facing state.

57 -terminal Trp8 to Ala both promote an inward-facing state.

58 es, i.e., the outward-facing (OF) and inward-facing (IF) states, as well as on the OF <-> IF state tr

59 alternate between their outward- and inward-facing conformations in the membrane.

60 rystal structures in the outward- and inward-facing conformations of a glutamate transporter homolog

61 ured in outward-facing, occluded, and inward-facing conformations.

62 an ZnT8 (HsZnT8) in both outward- and inward-facing conformations.

63 free and ions-only bound outward- and inward-facing conformations.

64 Whereas outward- and inward-facing GltPh conformations are nonconductive, lateral mo

65 ng occluded with substrate bound, and inward-facing open).

66 uctures of outward-facing Sav1866 and inward-facing Pgp.

67 transition between outward-facing and inward-facing states) scales.

68 tion of E374 in both the outward- and inward-facing states, hence the proton is not released in the e

69 tions, including both the outward and inward-facing states.

70 with height between the outward- and inward-facing states.

71 Comparison of these outward- and inward-facing structures reveal how the transport domain transl

72 A comparison of the outward- and inward-facing structures reveals that the TMD of each HsZnT8 su

73 It is defined by a "half-open and inward-facing" state (HOIF) of the intracellular gate that is s

74 leotide binding domains (NBDs) apart; inward-facing] and closed (NBDs close; outward-facing) conforma

75 outward-open conformation as well as inward-facing conformations.

76 structure, TmrAB adopts an asymmetric inward-facing state, and we show that the C-terminal helices, a

77 dicate that when the protomers become inward-facing, they cause deep, long-ranged, and yet mutually-i

78 pathway when the transporter becomes inward-facing.

79 making iterative transitions between inward-facing (E1) and outward-facing (E2) conformations.

80 by a Fab fragment, and a ligand-bound inward-facing (I(f)) conformation, possibly stabilized by a mut

81 er, LeuT, in a Na(+)/substrate-bound, inward-facing occluded conformation.

82 sporter protein in a substrate-bound, inward-facing state.

83 e to LeuT, promotes an outward-closed/inward-facing conformation of the transporter and increases upt

84 that result in a partially collapsed inward-facing substrate-binding cavity.

85 the activation of two cytoplasmically inward-facing channels: the plasma membrane, Cch1p, and the vac

86 arison with the previously determined inward-facing structure of SCL1A5 provides a basis for a more i

87 lubilized TmrAB in a nucleotide-free, inward-facing conformation by single-particle electron cryomicr

88 AT by inducing a relative increase in inward-facing transporters.

89 he crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the r

90 Furthermore, simulations of inward-facing LeuT with Na(+) ions and substrate bound suggest

91 xpected to increase the proportion of inward-facing transporters, whereas the reactivity of the mutan

92 lux involves changes between the open inward-facing (NBDs apart, extracellular loops (ECLs) close tog

93 crystal structure of MsbA in the open inward-facing conformation.

94 In this study, an open, inward-facing conformation of Pho84 was used to study the relea

95 ational change in Pho84 from an open, inward-facing state to an occluded state.

96 ng the stability of a partially open, inward-facing state.

97 conformational states: predominantly inward-facing apo P-gp, pre-hydrolytic (E552Q/E1197Q) P-gp boun

98 esting that Mhp1 adopts predominantly inward-facing conformations.

99 sidues selected to track the putative inward-facing to outward-facing transition.

100 binding site to the cytoplasmic side (inward-facing, open NBD conformation).

101 resolve the backbone dynamics in the inward-facing (IF) and outward-facing (OF) states by analyzing

102 enhanced solvent accessibility in the inward-facing (relative to the outward-facing) form.

103 Our results reveal that the inward-facing and outward-facing Mhp1 crystal structures repres

104 ucose EIIC superfamily, bcChbC in the inward-facing conformation and bcMalT in the outward-facing con

105 Next, starting from the inward-facing conformation of EcNhaA, we sample what could be i

106 nic binding of K(+) or of H(+) to the inward-facing conformation of SERT in (i) cancelling out the el

107 erse the conformation of MalFG to the inward-facing conformation, a step essential for release of mal

108 MalFG may be naturally stable in the inward-facing conformation, or the ABC domain may catalyze the

109 tion state structurally resembles the inward-facing conformation.

110 the nucleotide-binding domains in the inward-facing conformation.

111 Pho84 transport model is based on the inward-facing occluded crystal structure of the Pho84 homologue

112 until maltose triggers return to the inward-facing state for substrate and Pi release.

113 erconversion from the outward- to the inward-facing state in the conformational ensemble under ATP tu

114 from the outward-facing state to the inward-facing state involves rigid body movements of transmembr

115 gaine (a DAT ligand selective for the inward-facing state) and/or pifithrin-mu (an HSP70 inhibitor) r

116 l transition from the outward- to the inward-facing state, when the bound substrate is translocated f

117 ing by facilitating transition to the inward-facing state.

118 d converts the membrane domain to the inward-facing state.

119 for the release of the proton in the inward-facing state.

120 The inward-facing structure of PfMATE in combination with the outwa

121 cavity during the transition from the inward-facing to outward-facing conformational states, and its

122 omains during the transition from the inward-facing to outward-facing conformations.

123 phore decreases the prevalence of the inward-facing, but not the outward-facing state.

124 we present crystal structures of the inward-facing, intermediate, and outward-facing states of a con

125 environment must thus stabilize this inward-facing conformation for the transporter to function.

126 to investigate the properties of this inward-facing conformation in relation to transport by LeuT wit

127 change to similar extents relative to inward-facing P-gp.

128 d during the passage from outward- to inward-facing state (holo-occluded), and another, substrate-fre

129 nal transition from outward-facing to inward-facing state.

130 ctural isomerization from outward- to inward-facing states.

131 SUR1-ABC core is found in an unusual inward-facing conformation whereby the two nucleotide binding d

132 s destabilization at several of the 26 lipid-facing sites of PagP that we mutated in this study.

133 ical drug-binding pocket adjacent to a lipid-facing surface of the hERG1 channel, which furthers our

134 opyridines is located on the external, lipid-facing surface of the pore module, positioned at the int

135 sin by tryptophan substitution of four lipid-facing residues in transmembrane helix 4 (TM4) that is k

136 recedented details on the existence of lipid-facing allosteric binding sites outside the transmembran

137 show that the introduction of a single lipid-facing arginine residue near the middle of the beta barr

138 e energy for all 20 amino acids at the lipid-facing interface (DeltaDeltaG(0)w,i(phi)) and the protei

139 are most favorably transferred to the lipid-facing interface, whereas charged and polar groups displ

140 o tryptophan substitution, whereas the lipid-facing regions of TM3 and TM4 were variably tolerant.

141 h the partitioning free energy for the lipid-facing residue and inversely with the protein-facing res

142 ing process of an OMP is driven by the lipid-facing residues in its hydrophobic core, and its NC-IN t

143 oteins to the complete redesign of the lipid-facing surface of Escherichia coli OmpA.

144 In contrast, the lipid-facing surface of the last two BamA beta-strands forms w

145 and the design rules that govern this lipid-facing surface are poorly understood.

146 inding site in the cavity, adjacent to lipid-facing fenestrations proposed to be the portals for drug

147 erally applicable to drug targets with lipid-facing binding sites.

148 tions: cytoplasm-facing, occluded, and lumen-facing.

149 formation, and tetrabenazine binds the lumen-facing conformation.

150 r structural analysis of mCST's unique lumen-facing partially-occluded conformation, coupled with the

151 We identified residues in the matrix-facing region of Tim17 involved in binding of the import

152 ) code to systematically replace 29 membrane-facing leucine, isoleucine, valine, and phenylalanine re

153 red linker that becomes helical and membrane-facing in the open form of the AP2 clathrin adaptor comp

154 Basic membrane-facing residues of this helix are critical, as charge-re

155 at active Msp1 contains a conserved membrane-facing surface adjacent to a central pore.

156 val, loss of VDAC2 or replacing its membrane-facing glutamate with glutamine renders human colon canc

157 at well-defined locations on their membrane-facing surface.

158 emical differences between WSOM from a north-facing slope, south-facing slope, and riparian zone, the

159 s inhabiting the colder and more humid north-facing slope carried about 6% more transposable elements

160 On the north-facing one, Mucoromycota, primarily Mucor, were abundant

161 frican" slope (AS)], abutting with the north-facing slope forested south-European continent ["Europea

162 At these ocean-facing sites, deer samples had significantly higher THg

163 One protomer is in the outside-facing state.

164 Outward-facing structural changes in a bound small molecule can

165 in the protein being unable to adopt outward-facing conformations or in a global destabilization of s

166 We found a novel high affinity outward-facing conformational state of the human SERT induced by

167 what could be its long-sought-after outward-facing conformation and verify it using cross-linking ex

168 that R60A preferentially assumes an outward-facing (OF) conformation through compensatory intracellu

169 between an inward-facing (IF) and an outward-facing (OF) conformation.

170 substrate rhodamine 6G stabilizes an outward-facing (OF) conformation.

171 Both structures are in an outward-facing conformation, and hepcidin binds between the two

172 on of the TMD, from an inward- to an outward-facing conformation, is driven exclusively by ATP hydrol

173 ith the substrate, L-glutamine in an outward-facing conformation.

174 en interact with DAT to stabilize an outward-facing DAT conformation and enhance cocaine binding.

175 Electrons are channeled from an outward-facing formate dehydrogenase via menaquinones to a fumar

176 ward-facing crystal structure and an outward-facing model of a bacterial DASS family member, VcINDY f

177 symmetric conformations, yielding an outward-facing occluded state.

178 The structure is locked in an outward-facing open conformation by an inhibitor.

179 il of ATP in this structure is in an outward-facing position pointing away from the active site.

180 which the transporter returns to an outward-facing state after the transported substrate is released

181 a conformational transition from an outward-facing to an inward-facing state of the transporter.

182 -homologous transporter, promotes an outward-facing transporter conformation upon substrate binding,

183 tions between inward-facing (E1) and outward-facing (E2) conformations.

184 s), which switch between inward- and outward-facing (IF, OF) orientations.

185 transitions between inward- (IF) and outward-facing (OF) states ('alternating-access' mechanism).

186 namics in the inward-facing (IF) and outward-facing (OF) states by analyzing purified preparations of

187 P and alternates between inward- and outward-facing conformations during maltose transport.

188 that MurJ can adopt both inward- and outward-facing conformations in vivo Furthermore, we show that d

189 ge the energetics of the inward- and outward-facing conformations of Mhp1.

190 ternating access between inward- and outward-facing conformations of the substrate-loaded transporter

191 that alternates between inward- and outward-facing conformations to capture and force substrates out

192 ed, inward-open, inward-occluded and outward-facing conformations.

193 nes by switching between inward- and outward-facing conformations.

194 h those calculated using inward- and outward-facing crystal structures of MdfA, show that in detergen

195 ts reveal that the inward-facing and outward-facing Mhp1 crystal structures represent sampled interme

196 1197Q) P-gp bound to Mg(+2)-ATP, and outward-facing P-gp bound to Mg(+2)-ADP-VO(4)(-3).

197 the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter

198 erconversion between the inward- and outward-facing states of EmrE varies over 3 orders of magnitude.

199 tween conformations with inward- and outward-facing substrate-binding sites in response to engagement

200 l structures of MalFGK2, inward- and outward-facing, show that the transporter is sealed against ions

201 sure) and global (transition between outward-facing and inward-facing states) scales.

202 ward-facing] and closed (NBDs close; outward-facing) conformations.

203 ECLs) close together) and the closed outward-facing (NBDs close together, ECLs apart) conformations.

204 ed construction of a high-confidence outward-facing Pgp atomic model based on crystal structures of o

205 as been crystallized in three forms (outward-facing open, outward-facing occluded with substrate boun

206 ded SBP docks to the nucleotide-free outward-facing conformation of the transporter.

207 ggers conformational transition from outward-facing to inward-facing state.

208 e medium and normal skin to generate outward-facing gradients.

209 ugh to form a disulfide bond only in outward-facing and early intermediate conformations of the homol

210 The high degree of similarity in outward-facing structures across evolution of ABC transporters a

211 , the LeuT-fold has been captured in outward-facing, occluded, and inward-facing conformations.

212 g and locking the transporter in its outward-facing state.

213 shift in equilibrium to favor a more outward-facing conformation of SERT can explain the reduced turn

214 hat were sensitized to detect a more outward-facing conformation of SERT.

215 o guide site-directed mutagenesis of outward-facing hydrophobic residues within the transmembrane reg

216 model based on crystal structures of outward-facing Sav1866 and inward-facing Pgp.

217 in three forms (outward-facing open, outward-facing occluded with substrate bound, and inward-facing

218 termined in an inward-facing (IF) or outward-facing (OF) conformation.

219 switch SUR1 alone between inward- or outward-facing conformations with low or high dissociation const

220 , the protein adopts a predominantly outward-facing conformation, although more closed than the cryst

221 binding site, but also in a putative outward-facing selective gate.

222 nsporters are only known in V-shaped outward-facing conformations.

223 essibility of the drug-binding site (outward-facing, closed NBD conformation), and ATP hydrolysis lea

224 te that occupation of Na2 stabilizes outward-facing conformations presumably through a direct interac

225 jor conformational states, i.e., the outward-facing (OF) and inward-facing (IF) states, as well as on

226 ding of transition metal ions to the outward-facing apo state of DAT and a reciprocal interaction of

227 with a fully atomistic model of the outward-facing conformation in membranes, the insight into Pgp c

228 close proximity to each other in the outward-facing conformation of GAT-1.

229 state MalE stabilizes MalFGK2 in the outward-facing conformation until maltose triggers return to the

230 acing conformation and bcMalT in the outward-facing conformation, were previously solved.

231 rs suggest a small preference to the outward-facing crystal structure, with a somewhat more open conf

232 Compared to the outward-facing occluded conformation, our structure reveals a ma

233 re of PfMATE in combination with the outward-facing one supports an alternating access mechanism for

234 ng step where SERT may return to the outward-facing state in a KCl- or HCl-bound form.

235 pidly from the inward- than from the outward-facing state of DAT.

236 Transition from the outward-facing state to the inward-facing state involves rigid b

237 raction of transporters adopting the outward-facing state under different experimental conditions.

238 ed to protonate Gluex and enrich the outward-facing state, a residue ~20 A away from Gluex, near the

239 While in the outward-facing state, increased ECL exchange corresponding to EC

240 In the outward-facing state, the ICH-NBD1 interface showed decreased ex

241 glucose locks the transporter in the outward-facing state.

242 ce of the inward-facing, but not the outward-facing state.

243 ved near the domain interface in the outward-facing state.

244 n the inward-facing (relative to the outward-facing) form.

245 transition from the inward-facing to outward-facing conformational states, and its absence in the rev

246 transition from the inward-facing to outward-facing conformations.

247 are transferred from menaquinones to outward-facing CprA, via an as-yet-unidentified membrane complex

248 and a global reconfiguration back to outward-facing state to resume the transport cycle.

249 s a reorientation from an inward- to outward-facing state.

250 nformational change from occluded to outward-facing states is unusually simple, involving only the ro

251 track the putative inward-facing to outward-facing transition.

252 labeling, indicating a shift toward outward-facing conformations that can be interpreted using conve

253 ion of a previously uncharacterized 'outward-facing open' state, and highlight the relevance of globa

254 are positioned at the periphery with outward-facing active sites suggesting that the complex may targ

255 bout 100 contractile structures with outward-facing baseplates, linked by tail fibers and a dynamic h

256 de variety of clinical scenarios and patient-facing governance where only part of the VCF data is fit

257 agues highlight how the expansion of patient-facing electronic health record portals could exacerbate

258 A separate patient-facing registry characterized risk-mitigating behaviors.

259 age consultation duration, and total patient-facing clinical workload in English general practice.

260 double labeling of sites on the periplasmic-facing surface of BtuB is possible using a dsbA null str

261 may be a redeeming factor for Ta as a plasma-facing material.

262 en is the main candidate material for plasma-facing armour components in future fusion reactors.

263 al integrity of leading candidate for plasma-facing materials, tungsten (W), in future nuclear fusion

264 e for high-pressure helium bubbles in plasma-facing tungsten.

265 s work is to assess Ta as a potential plasma-facing material for future fusion reactors in terms of i

266 arge heat fluxes onto the surrounding plasma-facing components (PFCs).

267 kamak-relevant conditions on tungsten plasma-facing materials in a magnetic fusion energy device.

268 polar solvent, placed between two like-poles-facing NdFeB magnets, allowed separation of seven releva

269 In the modeled channel, pore-facing regions of TM1 and TM2 were highly sensitive to t

270 ticularly at Ser12, indicating that the pore-facing serine residues in BM2 mediate proton relay to th

271 clear that ionized side chains-whether pore-facing or buried-in the first alpha-helical turn of the

272 olvement of ionized side chains-whether pore-facing or buried-rather than backbone atoms and propose

273 ressed early between anterior- and posterior-facing wounds.

274 signaling in intact animals and at posterior-facing wounds.

275 amic contributions of the lipid- and protein-facing residues in the transmembrane protein beta-signal

276 ace (DeltaDeltaG(0)w,i(phi)) and the protein-facing interface (DeltaDeltaG(0)w,i(pi)) residues and fo

277 acing residue and inversely with the protein-facing residue.

278 As scientists respond to calls for public-facing communication, we need to evaluate where the scie

279 asolateral (blood-facing) and apical (saliva-facing) membranes of salivary gland acinar cells, sugges

280 n the DTC and Sh1, and Sh1 grew over the Sh1-facing daughter.

281 ter is an internally cooled, 14-French, side-facing catheter, integrated with A-mode ultrasound guida

282 y C1-oxidizing LPMO9s, access to the solvent-facing axial coordination position is restricted by a co

283 e population from the hotter and drier south-facing slope.

284 ements than those from the hot and dry south-facing slope, in parallel to a suite of other genetic an

285 Low-elevation, south-facing, nonconvergent locations with limited upslope wat

286 es had a strong general preference for south-facing slopes, where thermal uplift develops predictably

287 otically, representing the dry and hot south-facing slope savannoid-African continent ["African" slop

288 etween WSOM from a north-facing slope, south-facing slope, and riparian zone, there were clear differ

289 s and showed denser connections at the south-facing slope.

290 phytic OTUs were characteristic of the south-facing slope.

291 e effect of slope exposure (north- vs. south-facing slope) on the decomposition of Picea abies wood b

292 ates non-integrally with intermembrane space-facing membranes and assembles in a range of complexes.

293 wever, parasitic light absorption in the sun-facing front molecular layer, through which sun light mu

294 An integrated, surgeon-facing, point-of-care risk prediction instrument was cre

295 coupled to online GC; and due to its upward-facing design, the electrode surface is less prone to bl

296 ices are deployed as containers and the user-facing interfaces as web-based applications.

297 appendages that bear well-developed ventral-facing spines, paired dorsal spines on the trunk, and po

298 ltaT 0-50 for south-, north-, east- and west-facing sides of buildings were highest on sunny days in

299 d 0.20 m for south-, north-, east-, and west-facing walls.

300 ts on temperature of solar radiation on west-facing slopes.