ライフサイエンスコーパス: 2-mercaptoethanol

1 ng thiol like DTT (dithiothreitol) and 2-ME (2-mercaptoethanol).

2 strongly inhibited by high concentrations of 2-mercaptoethanol.

3 is significantly quenched by 450-fold excess 2-mercaptoethanol.

4 activate GABA-AT, but only in the absence of 2-mercaptoethanol.

5 dependent inhibitor, even in the presence of 2-mercaptoethanol.

6 th o-phthalaldehyde (OPA) in the presence of 2-mercaptoethanol.

7 ation by H2O2 or reduction by glutathione or 2-mercaptoethanol.

8 mercuric chloride was readily reversible by 2-mercaptoethanol.

9 nd destabilized by D-ribulose-5-phosphate or 2-mercaptoethanol.

10 be completely suppressed by the addition of 2-mercaptoethanol.

11 vity of FDH occurred only in the presence of 2-mercaptoethanol.

12 s is subsequently achieved with an excess of 2-mercaptoethanol.

13 ted to the 31-kDa band by the reducing agent 2-mercaptoethanol.

14 e GR active site with thiol reagents such as 2-mercaptoethanol.

15 (300 microM) and the lipophilic antioxidant 2-mercaptoethanol (10 microM) both protected significant

16 100 (20 mm), PA (2 mm), Mg(2+) (0.5 mm), and 2-mercaptoethanol (10 mm) at pH 7.5 and 30 degrees C.

17 nd L(R, S) recovered fully after transfer to 2-mercaptoethanol (10 mM).

18 Treatment of these sera with 2-mercaptoethanol (2-ME) reduced, but did not eliminate,

19 than structurally similar monothiols such as 2-mercaptoethanol (2-ME), that cleavage by DTT exhibits

20 elation coefficient between IgG anti-LPS and 2-mercaptoethanol (2-ME)-resistant vibriocidal antibodie

21 .0 unit/mL) with heat (95 degrees C, 5 min), 2-mercaptoethanol (2-ME, 0.83 %), and l-cysteine (l-Cys,

22 by epidermal growth factor, we found that 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone or Compou

23 A synthetic vitamin K analogue, 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone or compou

24 shown that a synthetic vitamin K analog, 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone or compou

25 Compound 5 (Cpd 5) or 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone, is an in

26 probed the effects of a Cdc25 inhibitor, 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone, or Compo

27 Treatment of protein samples with 2-mercaptoethanol abolished the S-thiolation signals det

28 n of the NCS-chrom C-6 radical, generated by 2-mercaptoethanol activation, to C-5 of the uracil at th

29 presumably due to their purification (i.e., 2-mercaptoethanol adducts and carbamylation) and related

30 ts such as tris(2-carboxyethyl)phosphine and 2-mercaptoethanol also abrogate NS5A-NS5A interactions,

31 y-7,8-dihydrobenzo[a]pyrene was trapped with 2-mercaptoethanol and characterized as a thioether conju

32 activity was demonstrated in the presence of 2-mercaptoethanol and dithiothreitol.

33 Two thiols, 2-mercaptoethanol and mercapto-2-propanol, were poorer s

34 K activation was blocked by the antioxidants 2-mercaptoethanol and N-acetyl-L-cysteine, suggesting th

35 M Tris (pH 7.5), 6 mM NaCl, 5 mM MgCl2, 5 mM 2-mercaptoethanol, and 10% (v/v) glycerol, 4 degrees C].

36 ris-HCl, pH 7.5, 6 mM NaCl, 5 mM MgCl2, 5 mM 2-mercaptoethanol, and 10% (v/v) glycerol, 4.0 degrees C

37 was observed with cysteine, dithiothreitol, 2-mercaptoethanol, and 3-mercaptopropionate.

38 The CoM analogues 2-mercaptopropionate, 2-mercaptoethanol, and cysteine substituted poorly for C

39 activation of spinach PRK by dithiothreitol, 2-mercaptoethanol, and glutathione was examined.

40 ut not with sodium dodecyl sulfate (SDS) and 2-mercaptoethanol, and migrated as polydisperse, high-mo

41 Reducing agents, such as DTT, 2-mercaptoethanol, and thioredoxin, were able to activat

42 tivity toward thiols like dithiothreitol and 2-mercaptoethanol as well as reagents: mixed disulfides

43 2-Mercaptoethanol assists in the conversion of monomers

44 The free radical scavenger 2-mercaptoethanol completely suppressed ROS generation b

45 Proteins extracted from coats with urea and 2-mercaptoethanol could, after removal of urea by gel fi

46 hy/mass spectrometry of the o-phthalaldehyde/2-mercaptoethanol derivatives and identified as citrulli

47 2-Mercaptoethanol, glutathione, dithiothreitol, and thio

48 n the presence of Co(2+), Mn(2+)or Mg(2+)and 2-mercaptoethanol in cacodylate or HEPES buffer, pH 7.2,

49 Inclusion of 2-mercaptoethanol in the upper electrode buffer greatly

50 show that the use of a shorter diluent like 2-mercaptoethanol is more advantageous than using a long

51 To assess the efficacy of 2-mercaptoethanol/L-cysteine mixed disulfide (CySSME) as

52 r employing sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (ME) resulted in 39.5% and 29.5%, resp

53 T), 2-(diethylamino)ethanethiol (DEAET), and 2-mercaptoethanol (ME) with o-phthaldialdehyde in the pr

54 essfully as co-substrates were thiosulphate, 2-mercaptoethanol, mercaptoacetate and aminoethylthiopse

55 Heating and reduction with 2-mercaptoethanol of GIF resulted in the release of a ap

56 harsh conditions such as treatment with pure 2-mercaptoethanol or treatment with boiling water for 5

57 Pretreatment of corneas with 2-mercaptoethanol prevented the effects of Hg2+ on the p

58 wo other antioxidants, N-acetyl cysteine and 2-mercaptoethanol, reduced growth and viability of MO7e

59 The reducing agent 2-mercaptoethanol significantly attenuates filament asse

60 th compound 8a and subsequent treatment with 2-mercaptoethanol/sodium methoxide afforded the guanine

61 Upon reduction with 2-mercaptoethanol the mass of this product decreased to

62 some cysteine residues were derivatized with 2-mercaptoethanol to form S,S-(2-hydroxyethyl)thiocystei

63 tions of amino acids with o-phthaldialdehyde/2-mercaptoethanol to generate electroactive derivatives

64 2-Mercaptoethanol was a weak competitive inhibitor vs Co

65 A preincubation with 2-mercaptoethanol was also included for optimal permeabi

66 protein from B. subtilis cells when urea and 2-mercaptoethanol were used in breakage buffers, implyin

67 ch as acetate, and by thiols such as GSH and 2-mercaptoethanol, which disrupt its stabilizing double

68 n and FDH was independent of the presence of 2-mercaptoethanol while 10-FDDF dehydrogenase activity o

69 in PBS but became fluorescent after SDS and 2-mercaptoethanol, with a quenching capacity of 10-fold