ライフサイエンスコーパス: 5' splice site

1 effect by promoting utilization of the weak 5' splice site.

2 due to the SMN2 C>T mutation and suboptimal 5' splice site.

3 We propose a mechanism for selection of the 5' splice site.

4 resence of intronic motifs downstream of the 5' splice site.

5 de polymorphisms (SNPs) within the consensus 5' splice site.

6 he initial recruitment of U1snRNP to the PLP 5' splice site.

7 he U6 snRNP at the precursor mRNA (pre-mRNA) 5' splice site.

8 ed in the presence of the competing upstream 5' splice site.

9 splice site located downstream of a weakened 5' splice site.

10 reposition the substrate after cleaving the 5' splice site.

11 ide to induce the activation of the Bcl-x(s) 5' splice site.

12 urther induce the activation of the Bcl-x(s) 5' splice site.

13 reside before its release from the pre-mRNA 5' splice site.

14 wo splicing signals: the branchpoint and the 5' splice site.

15 serves as the nucleophile for attack on the 5' splice site.

16 philic attack of the branch adenosine at the 5' splice site.

17 ot interfere with U1 snRNP binding to the N1 5' splice site.

18 site sequence converted it into a functional 5' splice site.

19 of the spliceosomal U1 snRNP to a suboptimal 5' splice site.

20 tion of U1 small nuclear RNA with the intron 5' splice site.

21 ecruitment of the splicing complex to a weak 5' splice site.

22 nteractions with cis elements that overlap a 5' splice site.

23 ence forms a hairpin that weakly tethers the 5' splice site.

24 inding of U1-70K (snRNP70) to the downstream 5' splice site.

25 exons that fail to reconstitute an efficient 5' splice site.

26 r implicated in displacing U1 snRNP from the 5' splice site.

27 iting hnRNP A1 to a sequence upstream of the 5' splice site.

28 x at the branchpoint and the U1 snRNP at the 5' splice site.

29 xogenous guanosine nucleophile to attack the 5'-splice site.

30 NPs bound to pre-mRNA with one or two strong 5' splice sites.

31 rgely on the strength of the flanking 3' and 5' splice sites.

32 nes, but also can promote use of alternative 5' splice sites.

33 rotein particle called U1 snRNP engages with 5' splice sites.

34 by the altered splicing of introns with weak 5' splice sites.

35 exon splicing with RBM20 binding near 3' and 5' splice sites.

36 ion of cassette exons, and usage of upstream 5' splice sites.

37 extreme conservation of GpU observed at most 5'-splice sites.

38 fine-tunes relative affinities of mismatched 5'-splice sites.

39 onto pre-mRNA guided by specific sequences (5' splice site, 3' splice site, and branchpoint).

40 RBM25 stimulated proapoptotic Bcl-x(S) 5' splice site (5' ss) selection in a dose-dependent man

41 intron compensatory interactions between the 5' splice site (5'ss) and 3' splice site (3'ss) were obs

42 snRNPs) bound to the precursor messenger RNA 5' splice site (5'SS) and branch point sequence, associa

43 f a complex comprising U1 snRNP bound at the 5' splice site (5'SS) and the Msl5*Mud2 heterodimer enga

44 pre-mRNA splicing is the recognition of the 5' splice site (5'ss) by canonical base-pairing to the 5

45 the major splice donor in the HIV-1 genome, 5' splice site (5'ss) D1, to the first splice acceptor,

46 viral mRNAs, is increased by optimizing the 5' splice site (5'ss) downstream of exon 2 (5'ss D2).

47 an splicing factor, acts at the U1 snRNA and 5' splice site (5'ss) duplex in the pre-mRNA splicing pr

48 Transfer of the 5' splice site (5'SS) from U1 to U6 snRNA triggers unwin

49 ad a major effect on the U1 pattern, whereas 5' splice site (5'ss) mutations had a stronger effect on

50 encer N1 (ISS-N1), located downstream of the 5' splice site (5'ss) of exon 7.

51 The SPO22 intron has a non-consensus 5' splice site (5'SS) that dictates its Nam8/Mer1-depend

52 e splice complex that forms between a pseudo 5' splice site (5'ss) within the negative regulator of s

53 ribonucleoprotein (U1 snRNP) binding to the 5' splice site (5'SS), which is assisted by the Ser/Arg

54 the first catalytic step of splicing at the 5' splice site (5'ss).

55 y of the complex of U1 bound to the pre-mRNA 5' splice site (5'SS).

56 bicistronic pre-mRNA intron 1 contains three 5' splice sites (5' ss) and three 3' splice sites (3' ss

57 model human beta-globin gene with duplicated 5' splice sites (5'ss) and found that PTCs inserted betw

58 GC 5' splice sites (5'ss) are present in approximately 1% o

59 lly shorten the minimal distance between the 5' splice-site (5'SS) and branchpoint.

60 elinase 2 and that ceramides shift the Bcl-x 5'-splice site (5'SS) selection in favor of Bcl-x(S), we

61 tosis, and phenylketonuria, can be caused by 5'-splice-site (5'ss) mutations that are not predicted t

62 ch intron contains three obligate signals: a 5' splice site, a branch site, and a 3' splice site.

63 These include the 5' splice site, a guanosine at position 7, and the 5' sp

64 r data suggest a novel mechanism for exon 16 5' splice site activation in which the binding of RBFOX2

65 o a consensus sequence close to the Bcl-x(L) 5' splice site, altering the Bcl-x(L)/Bcl-x(S) ratio and

66 exon, mutually exclusive exons, alternative 5' splice site, alternative 3' splice site and retained

67 the first step of self-splicing, in which a 5'-splice site analogue (S) and guanosine (G) are conver

68 Comparisons of the affinities of 5'-splice site analogues that form only a subset of base

69 turation of yeast TERs such as an Sm site, a 5' splice site and a branch point, within the newly iden

70 d sequences include a u-G wobble pair at the 5' splice site and a guanosine in the omega position at

71 Interestingly, mutating BDF2 5' splice site and branch point consensus sequences part

72 involves U6atac and U12 interaction with the 5' splice site and branch site regions of a U12-dependen

73 The FRET measurements suggest that the 5' splice site and branch site remain physically separat

74 signals from the tiling array delineated the 5' splice site and branchpoint site, confirming predicte

75 Furthermore, we found that weak 5' splice site and large intron size are the determining

76 ing due to the activation of the overlapping 5' splice site and not to the changes in the integrase p

77 ce site, which greatly increases use of this 5' splice site and promotes gene expression.

78 ed through non-Watson-Crick pairing with the 5' splice site and the branch-point adenosine.

79 ion, the promoter region interacted with the 5' splice site and the terminator with the 3' splice sit

80 P to the 5' splice site no. 4, even when the 5' splice site and the U1 snRNA have been mutated to obt

81 s an intron in the E6 coding region with one 5' splice site and two alternative 3' splice sites, whic

82 we confirmed that U1 directly hybridizes to 5' splice sites and 5' splice site motifs throughout int

83 sed RNA polymerase II (Pol II) at promoters, 5' splice sites and active enhancers, and are processed

84 e, chromatin-bound lncRNAs are enriched with 5' splice sites and depleted of 3' splice sites, and exh

85 t U2AF(59)-insensitive introns have stronger 5' splice sites and higher A/U content.

86 ary structural elements near the alternative 5' splice sites and show that splice site choice is sign

87 nisms underlie G run-mediated recognition of 5' splice sites and that context and position play a cri

88 with increased affinity to HIV-1 downstream 5' splice sites and to sequences within the first tat co

89 olecular ruler whose other end specifies the 5'-splice site and that this molecular ruler is conserve

90 the requirement for DISE to be close to the 5'-splice site and the ability of DISE to promote bindin

91 (SL4) of the U1 snRNA, which recognizes the 5' splice site, and a component of the U2 small nuclear

92 s), contain cytosine-rich sequences near the 5' splice site, and have suboptimal splice sites in the

93 icing, when the branch site (BS) attacks the 5' splice site, and the second step, when the 5' exon at

94 erated via the utilization of an alternative 5' splice site, and this results in an insertion of 31 a

95 exon skipping, intron retention, alternative 5' splice sites, and alternative 3' splice sites.

96 ncers (ESEs) promoted use of intron-proximal 5' splice sites, and tethering of hnRNP A1 and SF2/ASF p

97 ting that non-Watson-Crick base pairs at the 5' splice site are acting in concert with other factors

98 We have investigated the reasons why 5' splice sites are not fixed in complex E, using single

99 liceosomes assembled on reporter genes whose 5' splice sites are predicted to bind poorly at the spli

100 plicated the U1 snRNP and recognition of the 5' splice site as key elements in Mer1p-activated splici

101 ons destabilizing a stem-loop protecting the 5' splice site at the E10/intron 10 junction.

102 g of pre-mRNAs by recognizing and binding to 5' splice sites at exon/intron boundaries.

103 spliced at the E6 and E1/E2 ORFs, where two 5' splice sites at nt 232 and nt 898 and two 3' splice s

104 . crassa TER intron contains a non-canonical 5'-splice site AUAAGU that alone prevents the second ste

105 nteraction of U1 we propose occurs before U1-5' splice site base pairing.

106 The U1 snRNA mutations occur in the 5' splice-site binding region, and snRNA-mutant tumours

107 In contrast, a non-canonical 5' splice site blocks the second transesterification rea

108 otifs that effectively silenced the proximal 5' splice site both in vitro and in vivo.

109 and A1 induce extended contacts between the 5' splice site-bound U1 snRNA and neighboring exonic seq

110 cific sequence elements, including conserved 5' splice sites, branch points, pyrimidine-rich regions

111 , the U1 snRNP binds properly to the N1 exon 5' splice site but is made inactive by the presence of P

112 inclusion in the context of the native weak 5' splice site, but not the engineered strong 5' splice

113 sed exons in the affected genes contain weak 5' splice sites, but are otherwise indistinguishable fro

114 ntronic U-rich motifs are separated from the 5' splice site by overlapping inhibitory elements.

115 e choice after the initial identification of 5' splice sites by U1 snRNP.

116 Genetic variants within the 5' splice site can cause splicing differences among huma

117 located 100 nucleotides downstream from the 5' splice site can dimerize in vitro through an intermol

118 demonstrated that activation of the Bcl-x(s) 5' splice site can increase the effectiveness of chemoth

119 tein particle (snRNP), bound to a downstream 5' splice site, can positively influence utilization of

120 ing, presumably by interacting with the weak 5' splice site, CCGU.

121 mechanisms for establishing the fidelity of 5' splice site cleavage and exon ligation share a common

122 6 was disabled, these spliceosomes catalyzed 5' splice site cleavage but at a reduced rate.

123 Disrupting stem IIc also exacerbated the 5' splice site cleavage defects of certain substrate mut

124 Prp16 can associate with spliceosomes before 5' splice site cleavage, consistent with a role for Prp1

125 splicing of a genuine substrate until after 5' splice site cleavage, we found that Prp16 can associa

126 istent with a role for Prp16 in proofreading 5' splice site cleavage.

127 in vitro assay sensitive to proofreading of 5' splice site cleavage.

128 xD/H box ATPase that promotes splicing after 5' splice site cleavage.

129 In particular, we detected 5'splice site cleavage by the spliceosome, showing that

130 ing motifs function by altering the U1 snRNP/5' splice site complex in a manner that impairs commitme

131 the presence of U1 snRNPs base-paired to the 5' splice site, components recognizing the 3' splice sit

132 Here, we test the 3' and 5' splice site contributions towards efficient exon reco

133 RNA such as cryptic 3' splice site, aberrant 5' splice site, cryptic branch point and an exonic splic

134 e of these mutants (NLD2up), the sequence of 5' splice site D2 was changed to a consensus splice dono

135 splice sites that define exon 3, 3'ss A2 and 5' splice site D3.

136 ocking the N1 splice sites, PTB prevents the 5' splice site-dependent assembly of U2AF into the E com

137 The data show that recognition of the DM20 5' splice site depends on G run-mediated recruitment of

138 es where Watson-Crick base pairs form at the 5'splice site do we see degradation of TES products at c

139 ck interactions between the branch helix and 5'-splice site dock the branch adenosine into the active

140 However, it is unknown if the 3' or the 5' splice site dominates the exon recognition process.

141 Five 5' splice sites (donor sites) and six 3' splice sites (a

142 uence of U6 snRNA, which base-pairs with the 5'-splice site during catalytic activation, forms a hair

143 also demonstrated that an upstream competing 5' splice site enhances the rate of proximal splicing.

144 e-binding region of U2 is used in trans as a 5' splice site for both steps of splicing in vivo.

145 tion between the presence of a non-canonical 5' splice site for ESP and the absence of detectable ESP

146 rm owing to competition with a reconstituted 5' splice site formed at the RS-site after the first spl

147 sion) assays show that part of the regulated 5'-splice site forms intramolecular base pairs that are

148 new splicing regulatory element, an upstream 5' splice site functioning as a splicing enhancer.

149 no-specific AGAA-rich ESEs coupled to a weak 5'-splice site functions as a responsive gauge.

150 Exon 16 possesses a weak 5' splice site (GAG/GTTTGT), which when strengthened to

151 ding on cassette exons: binding close to the 5' splice site generally promoted exon inclusion, wherea

152 e S. pombe TER intron contains the canonical 5'-splice site GUAUGU, the N. crassa TER intron contains

153 studied the most common form of alternative 5' splice sites-GYNNGYs (Y = C/T), where both GYs can fu

154 abilize a stem loop structure at the exon 10 5'-splice site; however, the existence of this stem loop

155 e oligonucleotides (ASO) directed toward the 5' splice site I promote the expression of the PKCdeltaV

156 with PKCdelta minigene promoted selection of 5' splice site II.

157 ' splice site, but not the engineered strong 5' splice site, implying that RBFOX2 achieves its effect

158 We identify a G-rich enhancer (M2) of DM20 5' splice site in exon 3B and show that individual G tri

159 lement located immediately downstream of the 5' splice site in intron 7.

160 determined that U1 snRNP interacts with the 5' splice site in the downstream exon even in the absenc

161 M20 ratio and, when placed between competing 5' splice sites in an alpha-globin minigene, direct hnRN

162 we determined that two competing alternative 5' splice sites in exon 12 yield Esrp1 isoforms with dif

163 ed by oligodendrocytes (OLs) by selection of 5' splice sites in exon 3.

164 U1 snRNAs associate with 5' splice sites in the form of ribonucleoprotein particl

165 19, and IE17.5 transcripts utilized the same 5'-splice site in exon 3.

166 MD, and (ii) RPS3 binding activates a poison 5'-splice site in its pre-mRNA that leads to a frame shi

167 The P1 extension forms at the 5'-splice site in the first step of self-splicing, and P

168 hnRNPH fusion protein downstream of the DM20 5' splice site increased DM20 splicing, whereas MS2-hnRN

169 r initial recruitment of U1snRNP to the DM20 5' splice site independent of the strength of the splice

170 s rely on hyperstabilization of the U6 snRNA-5' splice site interaction to impede the 2nd step of spl

171 1C and Cbp80p to help stabilize the U1 snRNP-5' splice site interaction.

172 Interestingly, the 5' splice site is required for MBNL1-mediated activation

173 In summary, AS at GYNNGY 5' splice sites is primarily splicing noise, and seconda

174 Alternative splicing of competing 5' splice sites is regulated by enhancers and silencers

175 nstream and potentially competitive stronger 5'-splice site is actively repressed.

176 The 5'-splice site is cleaved but remains close to the catal

177 nces DM20 splicing, whereas close to the PLP 5' splice site, it inhibits PLP splicing.

178 n exons and introns is more complex than for 5' splice sites, largely owing to sequence constraints u

179 s; the third mutation activated a downstream 5' splice site leading to pseudoexon inclusion of a port

180 poly(A) signal or increasing the strength of 5' splice site led to the predominant production of hERG

181 ng from a trans-splicing reaction in which a 5' splice site-like sequence in the universally conserve

182 lements that could silence a proximal strong 5' splice site located downstream of a weakened 5' splic

183 that retained introns tend to have a weaker 5' splice site, more Gs in their poly-pyrimidine tract a

184 1 directly hybridizes to 5' splice sites and 5' splice site motifs throughout introns and found that

185 A rescues splicing of certain branchpoint or 5' splice site mutants in which U snRNA base-pairing has

186 des most unspliced precursors generated by a 5' splice site mutation in RPS10B, and limits RPS29B uns

187 sense mutations, 9 frameshift mutations, and 5 splice-site mutations.

188 ently, certain exons are more susceptible to 5' splice site mutations.

189 U1 snRNP binding to the 5' splice site no. 4 is required for splicing of the rev

190 y for the recruitment of the U1 snRNP to the 5' splice site no. 4, even when the 5' splice site and t

191 nd binding of the U1 snRNP to the downstream 5' splice site no. 4.

192 We demonstrated that neither the 5' splice site nor exon 11 sequences are required for MB

193 binding assays, shows that the selection of 5'-splice site nucleotides by U1 snRNP is achieved predo

194 short RNA duplex is established between the 5' splice site of a pre-mRNA and the 5' end of the U1 sn

195 PKCdeltaVIII via utilization of a downstream 5' splice site of exon 10 on PKCdelta pre-mRNA.

196 tations were identified over time except for 5' splice site of exon 10.

197 onal only in the context of the natural weak 5' splice site of exon 2; they are 1) a uridine-rich enh

198 U-rich intronic sequence (IAS1) adjacent the 5' splice site of exon IIIb.

199 (CBC), preferentially affecting selection of 5' splice site of first introns.

200 of HSF4 showed a homozygous mutation in the 5' splice site of intron 12 (c.1327+4A-->G), which cause

201 tation is due to a G --> A transition at the 5' splice site of intron 6 of SHM1, causing aberrant spl

202 sequence in the U1 snRNA, which binds to the 5' splice site of introns, effectively reversing the rol

203 teractions within U1 snRNP, and show how the 5' splice site of pre-mRNA is recognised by U1 snRNP.

204 ive splicing is a stem-loop structure at the 5' splice site of tau exon 10.

205 reby increasing the access of U1snRNP to the 5' splice site of tau exon 10.

206 r of miRNAs occurs in close proximity to the 5' splice site of the previously identified 7.2-kb stabl

207 When the unregulated 5' splice site of the upstream exon 3 is present, U2AF b

208 We proved that the 5' splice sites of CD46 cassette exons 7 and 8 encoding

209 (3aAG>AT) containing a point mutation in the 5' splicing site of exon 3a (3aAG > AT); and (iii) Ccalp

210 S1+1G-->A) which is predicted to destroy the 5'-splice site of exon 1 and remove the start methionine

211 utations in tau are silent, are found at the 5'-splice site of exon 10, and lead to increased inclusi

212 se that the UAYYUU motif hybridizes with the 5' splice site on the SL2 RNA to recruit the SL to the p

213 well as the positive effect of a downstream 5' splice site on trans-splicing in nematode extracts co

214 ns and demonstrated that mutation of the E1B 5' splice site or branchpoint abrogates intrasplicing.

215 site, with only interactions closest to the 5' splice site persisting to the 5' exon intermediate an

216 oned redundantly to protect an exon from its 5' splice site polymorphism.

217 s widespread context-dependent robustness to 5' splice site polymorphisms in human transcriptomes.

218 fied signals that could modify the impact of 5' splice site polymorphisms, most notably a strong 3' s

219 ption-coupled splicing of RNAs in which weak 5' splice sites predominate, enabling diversification of

220 nteractions with the 5' exon adjacent to the 5' splice site, prior to the first step of splicing.

221 at 7 out of 16 base pair combinations at the 5' splice site produce appreciable TES product.

222 e-messenger RNA (pre-mRNA) splicing requires 5' splice site recognition by U1 small nuclear RNA (snRN

223 regulate the PLP/DM20 ratio by reducing DM20 5' splice site recognition by U1snRNP.

224 nd timing of snRNA interactions required for 5' splice site recognition prior to the first step of pr

225 f splicing could reveal important signals of 5' splice site recognition.

226 U1 snRNP plays a critical role in 5'-splice site recognition and is a frequent target of a

227 n, and that this interaction interferes with 5'-splice-site recognition by the U1 snRNP.

228 finally contacts U1-C, which is crucial for 5'-splice-site recognition.

229 ssembly and suggests a possible mechanism of 5'-splice-site recognition.

230 iple steps by re-splicing at ratchet points--5' splice sites recreated after splicing.

231 endonuclease and show that catalysis at the 5' splice site requires the conserved cation-pi sandwich

232 either 3' splice site or near the downstream 5' splice site, respectively.

233 SM induces splicing of STAT1 at a novel 5' splice site, resulting in a STAT1 mRNA incapable of p

234 T mutation activates an internal alternative 5' splice site, resulting in formation of the disease-ca

235 477C>T (3849+10 kb C>T), which creates a new 5' splice site, resulting in splicing to a cryptic exon

236 al U1-snRNP proteins in governing the fungal 5' splice site RNA-RNA interaction compared to the numbe

237 conserved catalytic triad is protonated upon 5'-splice-site scission, promoting a reversible structur

238 ly, untranslated region activated HPV16 late 5'-splice site SD3632 and resulted in production of HPV1

239 NAs that bypassed the suppressed HPV16 late, 5'-splice site SD3632; produced elevated levels of RNA-b

240 Together, these results demonstrate that 5' splice site selection in LMNA is determined by an int

241 Alternative 5' splice site selection is one of the major pathways re

242 The underlying mechanism for this 5' splice site selection is unknown.

243 RCE 1, functions to regulate the alternative 5' splice site selection of Bcl-x pre-mRNA, and is requi

244 nts using pre-mRNAs that undergo alternative 5' splice site selection or alternative exon inclusion.

245 To identify the cis-elements involved in 5' splice site selection we cloned a minigene, which inc

246 totic Bcl-x(L), are produced via alternative 5' splice site selection within exon 2 of Bcl-x pre-mRNA

247 at the level of RNA splicing via alternative 5' splice site selection within exon 2 to produce either

248 ry demonstrated that ceramide regulated this 5' splice site selection, inducing the production of Bcl

249 Because snRNP70 is a key early regulator of 5' splice site selection, our results suggest a model in

250 and antiapoptotic Bcl-x(L), via alternative 5' splice site selection.

251 site with a concomitant increase in Bcl-x(s) 5' splice site selection.

252 The lariat is important for the fidelity of 5' splice-site selection and consists of a 2'-5' phospho

253 r, a double-point mutation in the NRS pseudo-5' splice site sequence converted it into a functional 5

254 The unique N. crassa TER 5'-splice site sequence is evolutionarily conserved in T

255 urther show that alternative exons with weak 5' splice site sequences specifically show a strong corr

256 ched motif downstream of exons unaffected by 5' splice site SNPs.

257 n of this additional SR protein contacts the 5' splice site specifically in the mature spliceosome.

258 we have previously demonstrated that a weak 5' splice site (ss) serves as the major cause of skippin

259 suboptimal features, which cause the intron 5' splice site (ss) to interact weakly with U1 snRNA and

260 ription/splicing factor, associates with the 5' splice site (SS) within large complexes present in He

261 The NRS acts as a pseudo 5' splice site (ss), and serine-arginine (SR) proteins,

262 A splicing, when the branch site attacks the 5' splice site (SS), and the second step, when the 5' ex

263 spliceosome complexes revealed mechanisms of 5'-splice site (ss) recognition, branching, and intron r

264 alternative exons than either the 3' or the 5' splice site strength alone.

265 ach, we demonstrate that the combined 3' and 5' splice site strengths of internal exons provide a muc

266 hPrp8, did not cross-link to the NRS pseudo-5' splice site, suggesting that the tri-snRNP complex wa

267 es tend to be single stranded, and effective 5' splice sites tend to be double stranded.

268 and U1/U11 snRNPs and functions as a pseudo-5' splice site that interacts with and sequesters 3' spl

269 1 pre-mRNA, directing utilization of a novel 5' splice site that is used only in the presence of SM.

270 on, as well as strong secondary structure at 5' splice sites that correlates with unspliced events.

271 he spliceosome work together to identify the 5 splice site, the 3 splice site, and the branchsite (BS

272 lose proximity to either the DM20 or the PLP 5' splice site, the ISE recruits U1snRNP to both splice

273 abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third

274 2 increases U1 snRNP recruitment to the weak 5' splice site through direct interaction between its C-

275 ice site, a guanosine at position 7, and the 5' splice site-to-branch point sequence context.

276 II promoter, a neomycin-resistance gene, and 5'-splice site) typically produced mutagenized clones in

277 tating splice site selection than the actual 5' splice site/U1 snRNA base pairing potential.

278 prevents its interaction with the authentic 5' splice site upstream of the NRS.

279 the Ralgps2 mRNA and a shift in alternative 5' splice site usage in the Bptf mRNA.

280 ied out a systematic analysis of alternative 5' splice site usage using in vitro splicing assays.

281 y sequence of the pre-mRNA is a predictor of 5' splice site usage, with the distal position favored o

282 Alternative 5' splice site utilization in the minigene was promoted

283 ownstream of a heterologous exon with a weak 5' splice site was capable of repressing exon inclusion.

284 the patterns of nuclease protection at this 5' splice site, we find that the interaction of U1 is al

285 Weak U1 snRNA interactions with the 5' splice site were significantly rescued by the proximi

286 plex E most transcripts with two alternative 5' splice sites were bound by two U1 snRNPs.

287 , except for those that were adjacent to the 5' splice site where they had the opposite effect.

288 rved sequence element (alpha) located near a 5' splice site, which greatly increases use of this 5' s

289 plicing sequence in intron 2 adjacent to the 5' splice site, which is essential in regulating exon 2

290 n unusual splice-site mutation in the exon 1 5' splice site, which leads to loss of the Kozac sequenc

291 omopolymers (polyribonucleotides) and to the 5'-splice site, which overrides the binding of SC35 to t

292 s prevent binding of U1 and U6 snRNPs to the 5' splice site, while TIAR increases binding.

293 by diminishing the selection of the Bcl-x(L) 5' splice site with a concomitant increase in Bcl-x(s) 5

294 docking, and restructures the pairing of the 5' splice site with the U6 snRNA ACAGAGA region.

295 ms, PLP1 and DM20, due to alternative use of 5' splice sites with the same acceptor site in intron 3.

296 of KDR is activated by blocking the upstream 5' splicing site with an antisense morpholino oligomer.

297 rosslinking group is placed further from the 5' splice site, with only interactions closest to the 5'

298 his sequence shifts splicing to a downstream 5' splice site within a heterologous pre-mRNA substrate

299 nuclear RNA and ten proteins, recognize the 5' splice site within precursor messenger RNAs and initi

300 IE9 utilized a cryptic 5'-splice site within exon 3.