ライフサイエンスコーパス: ATP analog

1 f ATP as well as AMP-PCP (a non-hydrolyzable ATP analog).

2 odulated by the polynucleotide substrate and ATP analog.

3 tivity to competitive inhibition via a bulky ATP analog.

4 analyzed to find an efficient unhydrolyzable ATP analog.

5 number in the presence of a nonhydrolyzable ATP analog.

6 tubule-attached, dimeric kinesin bound to an ATP analog.

7 benzoyladenosine, an irreversible inhibitory ATP analog.

8 trand with duplex DNA in the presence of the ATP analog.

9 lication of ATP but not by a nonhydrolyzable ATP analog.

10 nts containing AMPPNP, a slowly hydrolyzible ATP analog.

11 at the engineered v-Src uniquely accepted an ATP analog.

12 triphosphate, gamma-S-ATP, a nonhydrolyzable ATP analog.

13 ompassing all six subunits, upon binding the ATP analog.

14 receptor with AMPPNP, a hydrolysis-resistant ATP analog.

15 ed by binding of a specific non-hydrolyzable ATP analog.

16 nce of both phosphate and a non-hydrolysable ATP analog.

17 ce of added nucleotide or the presence of an ATP analog.

18 by another kinase that could not utilize the ATP analog.

19 and ssDNA in the presence and absence of an ATP analog.

20 as well as that bound to a non-hydrolyzable ATP analog.

21 ion showed intermediate sensitivity to these ATP analogs.

22 g studies using fluorescent and spin-labeled ATP analogs.

23 eriments and inhibition with nonhydrolyzable ATP analogs.

24 s in the presence of ATP and nonhydrolyzable ATP analogs.

25 ressed in the form of reductions in kcat for ATP analogs.

26 e obtained with either peptide inhibitors or ATP analogs.

27 th neutral-backbone DNA and non-hydrolyzable ATP analogs.

28 combination of peptide and non-hydrolysable ATP analogs.

29 changes in ternary complexes with different ATP analogs.

30 of the Pat1 kinase (pat1-as2) by adding the ATP analog 1-NM-PP1 in G1-arrested cells allows the indu

31 e required to activate the receptor, and the ATP analog 2',3'-O-(4-benzoyl-benzoyl)ATP (BzATP) is bot

32 Finally, we demonstrate that the fluorescent ATP analog 2'/3'-O-(N'-methylanthraniloyl)-ATP (mantATP)

33 The C-2-modified ATP analogs 2-amino-ATP and 2-chloro (Cl)-ATP were excel

34 ers in rigor were labeled with a fluorescent ATP analog, 3'-DEAC-propylenediamine (pda)-ATP (3'-O-{N-

35 GRK5 in complex with the ATP analog 5'-adenylyl beta,gamma-imidodiphosphate or th

36 nosine triphosphate, and the nonhydrolyzable ATP analog 5'-adenylyl-beta,gamma-imidodiphosphate (AMP-

37 presence and absence of the nonhydrolyzable ATP analog 5'-adenylyl-beta,gamma-imidodiphosphate (AMPP

38 Moreover, Top2 bound to the nonhydrolyzable ATP analog 5'-adenylyl-beta,gamma-imidodiphosphate exhib

39 ctivity in the presence of the non-substrate ATP analog 5'-adenylyl-beta,gamma-imidodiphosphate verif

40 Similarly, Cak1p is insensitive to the ATP analog 5'-fluorosulfonylbenzoyladenosine, which inhi

41 iminated by substituting the nonhydrolyzable ATP analog 5-adenylyl-imidodiphosphate or UTP for ATP in

42 hydrolyze ATP, or to bind a nonhydrolysable ATP analog, 5'-adenylyl-beta,gamma-imidodiphosphate (ADP

43 Chemical modification with the ATP analog, 5'-p-fluorosulfonylbenzoyladenosine, showed

44 We show that JFC1 specifically binds to the ATP analog 8-azido-[alpha-(32)P]ATP.

45 enol ATP, as well as with a photoactivatable ATP analog, 8-azido-ATP (N(3)-ATP).

46 mass spectrometry using a novel fluorescent ATP analog, 8-azido-ATP-[gamma]-1-naphthalenesulfonic ac

47 he presence and absence of a nonhydrolyzable ATP analog a catalytically incompetent TTM tunnel domain

48 Upon binding to the ATP analog, a 100-fold reduction in affinity for ssDNA w

49 in ternary complex with the nonhydrolyzable ATP analog adenosine 5'-(beta,gamma-imido)-triphosphate

50 oli PhoQ complexed with the non-hydrolyzable ATP analog adenosine 5'-(beta,gamma-imino)triphosphate a

51 The enzyme co-crystallized with the ATP analog adenosine 5'-[gamma-thio]triphosphate contain

52 ctivity is inhibited by the non-hydrolysable ATP analog (adenosine 5'-O-(thiotriphosphate)), T4 singl

53 ATP analogs (adenosine 5'-(alpha, beta-methylene)triphos

54 The ATP analogs (adenosine 5'-O-(3-thiotriphosphate) or 5'-a

55 h or without adenosine or a non-hydrolyzable ATP analog, adenosine 5'-(gamma-thio)-triphosphate (ATPg

56 structure, in complex with a nonhydrolyzable ATP analog, adenosine 5'-adenylyl-beta,gamma-imidodiphos

57 nding in the presence of the nonhydrolyzable ATP analog, adenosine 5'-O-(3-thio)triphosphate (ATPgamm

58 Using either ATP or the non-hydrolyzable ATP analog, adenosine 5'-O-(3-thiophosphate), events in

59 ants, L273A and L108A, and a nonhydrolyzable ATP analog, adenosine 5'-O-(3-thiotriphosphate) (ATPgamm

60 RepA in the presence of a poorly hydrolyzed ATP analog, adenosine 5'-O-(thiotriphosphate), and to re

61 zation was abrogated in vitro by ATP and the ATP analog adenyl-5'-yl imidodiphosphate.

62 nced upon the addition of a non-hydrolyzable ATP analog (adenylyl-imidophosphate), whereas ADP had no

63 The ATP analog, adenylyl methylenediphosphonate (AMP-PCP), a

64 presence and absence of the non-hydrolyzable ATP analog ADP(BeF3).

65 Pase domain, bound with the non-hydrolyzable ATP analog ADP-beryllium fluoride, we studied the NtrC1-

66 teins in the presence of the nonhydrolyzable ATP analog ADP-beryllium fluoride.

67 ADP.Pi analogs ADP.AlF4 and ADP.Vi, and the ATP analogs ADP.BeFx, AMPPNP and ATPgammaNH2, all induce

68 only in the presence of the non-hydrolyzable ATP analog, ADP(BeF3).

69 nucleoside triphosphates, a nonhydrolyzable ATP analog, ADP, or AMP had no effect.

70 Another ATP analog, ADP-aluminum fluoride, does not promote unwi

71 hile in the presence of the non-hydrolyzable ATP analog, ADP-beryllium fluoride, we observe additiona

72 A nonhydrolyzable ATP analog, ADPCP (beta,gamma-methylene adenosine 5'-tri

73 rate that the presence of a non-hydrolyzable ATP analog allows Mtr4p to discriminate between partial

74 mammalian cartilage and bone, the effects of ATP analogs, ALP substrates, and specific inhibitors on

75 trate (2S,5S)-5-carboxymethylproline (CMPr), ATP analog alpha,beta-methyleneadenosine 5'-triphosphate

76 A single binding site for the ATP analog, alpha,beta-methylene ATP (Ap(CH2)pp), was al

77 ose homo-oligomers that are sensitive to the ATP analog alphabeta-methylene ATP(alphabetameATP) (P2X(

78 ATP and a nonhydrolyzable ATP analog also influence the stability of the DNA-PKcs*

79 p did not catalyze the hydrolysis of ATP and ATP analogs, although fluorescence measurements indicate

80 f human BRAF(KD) in complex with MEK and the ATP analog AMP-PCP, revealing interactions between BRAF

81 e investigated whether interactions with the ATP analog AMP-PNP and ADP can shift the conformational

82 The nonmetabolizable ATP analog AMP-PNP cannot be substituted for ATP in this

83 cellular perfusion with the non-hydrolyzable ATP analog AMP-PNP dramatically reduce the amplitude of

84 30 s, whereas those with the nonhydrolyzable ATP analog AMP-PNP had a mean lifetime of 4.8 +/- 0.7 mi

85 orylated, inactive state bound to either the ATP analog AMP-PNP or to one of the two small-molecule i

86 hondrial ATP, and cocrystallization with the ATP analog AMP-PNP suggests that binding of nucleotides

87 data establish that, in the presence of the ATP analog AMP-PNP, or ADP, a maximum of six DnaC monome

88 f the receptor with the hydrolysis-resistant ATP analog AMP-PNP.

89 ch has altered ligand selectivity toward the ATP analog AMP-PNP.

90 shigelloides in complex with GlcNAc and the ATP analog AMP-PNP.

91 6p complexed with an RNA oligonucleotide and ATP analogs AMP-PNP, ADP-BeF(3)(-), or ADP-AlF(4)(-).

92 te (Vi), acetate, ATP, or a non-hydrolyzable ATP analog (AMP-PNP), with differential effects on matri

93 The inability of the nonhydrolyzable ATP analog, AMP-PNP, to cause a similar effect is explai

94 the presence or absence of a nonhydrolyzable ATP analog, AMP-PnP.

95 of the open state bound to a nonhydrolyzable ATP analog (AMPPCP) and 1,6-anhydroMurNAc provide detail

96 e presence and absence of a non-hydrolyzable ATP-analog, AMPPCP.

97 The ATP analog, AMPPNP, protects probes in the active site a

98 pon exchange of ATP with the nonhydrolyzable ATP analog and ATP hydrolysis.

99 resence of hydrogen peroxide and AMP-PNP, an ATP analog and competitive inhibitor of ATPases.

100 (CX3) X-ray co-crystal structure with bound ATP analog and define separable RAD51C replication stabi

101 e in a quaternary complex with tRNA(Gln), an ATP analog and glutamate reveals that the non-cognate am

102 f activated NTPDase3 with a non-hydrolyzable ATP analog and the cofactor Mg(2+) to a resolution of 2.

103 ed here, of bovine F1-ATPase inhibited by an ATP analog and the phosphate analog, thiophosphate, repr

104 or its nucleotide substrate was tested using ATP analogs and alternative nucleotide donors.

105 ith ligand-gated P2X1 receptors activated by ATP analogs and high levels of ATP.

106 rystal structures of Mss116p in complex with ATP analogs and single-stranded RNA show that the helica

107 domains of AC in complex with two different ATP analogs and various divalent metal ions.

108 as mimicked by several sterically restricted ATP analogs and was blocked by suramin.

109 AMP-PNP (gamma-imino ATP, a nonhydrolyzable ATP analog) and Mg(2+)/ADP.

110 microM in the presence of a nonhydrolyzable ATP analog, and 45 microM in the presence of ADP or no n

111 -adenylylimidodiphosphate, a nonhydrolyzable ATP analog, and was blocked in the presence of H7 or the

112 plants display protein kinase activity, bind ATP analogs, and possess C-terminal domains similar to b

113 ment formed on DNA in the presence of ATP or ATP analogs, and this has been studied at low-resolution

114 n post-rigor transition state that binds the ATP analog AppNHp.

115 Modified ATP analogs are described that do not activate either co

116 p specifically requires ATP; nonhydrolyzable ATP analogs are ineffective.

117 e intrinsic affinities of all of the studied ATP analogs are lower than the intrinsic affinities of t

118 , the detection of which can be modulated by ATP analogs as well as DNA sequence flanking the TATA se

119 ither a SMG1 inhibitor or a non-hydrolyzable ATP analog at overall resolutions ranging from 2.8 to 3.

120 AMP-PNP, a nonhydrolyzable ATP analog, at a concentration of 1 or 3 mM was unable t

121 e interrupted by adding the non-hydrolyzable ATP analog ATP-gamma-S.

122 In contrast, the poorly hydrolyzed ATP analog ATP-gammaS only partially stabilizes the nucl

123 ional changes in the loop that surrounds the ATP analog (ATP-lid) and has implications for interactio

124 We demonstrate that the nonhydrolyzable ATP analog, ATP gamma S, supports the formation of an is

125 we established the gamma-phosphate-modified ATP analog, ATP-biotin, as a cosubstrate for phosphorylb

126 he E. coli clamp-loader complex bound to the ATP analog ATPgammaS (at a resolution of 3.5 A) and ADP

127 The ATP analog ATPgammaS is a competitive inhibitor of the r

128 addition of an excess of the unhydrolyzable ATP analog ATPgammaS, supplementation with exogenous ATP

129 Using the ATP analog ATPgammaS, we showed that ATP hydrolysis is r

130 esolution structure of the core bound to the ATP analog ATPgammaS.

131 rlc) binding to actin in the presence of the ATP analog ATPgammaS.

132 The adenosine triphosphate (ATP) analog ATPgammaS often greatly slows or prevents en

133 his complex reveal that the non-hydrolyzable ATP analog, ATPgammaS, induces a high-affinity binding m

134 nucleotide, or in the presence of ADP or the ATP analog, ATPgammaS, there was no helical ordering.

135 Binding of the ATP analog, ATPgammaS, tightly restructures the Walker A

136 unctional cycle by use of a non-hydrolyzable ATP analog, ATPgammaS, to mimic the ATP-bound GroEL stat

137 ures of Pho85-Pcl10 and its complex with the ATP analog, ATPgammaS.

138 bound state as well as in complexes with the ATP analog beta-gamma-methylene adenosine triphosphate (

139 IC50 of inhibitors using the nonhydrolyzable ATP analog, beta, gamma-methyleneadenosine 5'-triphospha

140 ATP analogs bind MRP1 with reduced apparent affinity, in

141 ATP analog binding to either site diminishes the intrins

142 e have previously shown that nonhydrolyzable ATP analogs block the lytic activity of NK cells and CD8

143 that binds ADP in bovine F(1) ATPase has an ATP analog bound and therefore this structure does not r

144 .ATP, and we observe a single molecule of an ATP analog bound in the aforementioned surface cavity, n

145 e the effect of force on the lifetime of the ATP analog bound to the actomyosin complex.

146 plex, deiNOS quenches the fluorescence of an ATP analog bound to TrpRS II, and increases its affinity

147 monomeric, AMP-PNP-DnaA (a non-hydrolysable ATP-analog bound-DnaA) was oligomeric, primarily dimeric

148 ences compared to AMPPNP (a non-hydrolyzable ATP analog) bound to PhoQcat and radicicol bound to Hsp9

149 ene)triphosphate (AMP-PCP) (non-hydrolyzable ATP analog) bound were also solved at 1.9-A resolution.

150 Non-hydrolyzable ATP analogs (but not ATP or ADP) release P1 from the pro

151 nzyl)-ATP and N(6)(phenethyl)-ATP over other ATP analogs, but still retained a 30 microm K(m) for ATP

152 The ATP analog, Bz-ATP, was a less effective agonist than AT

153 In the presence of ADP or ATP analogs, calcium increased the asymmetry of the HMM

154 n allostery not catalysis, and the classical ATP-analog class of tyrosine kinase inhibitors fail to i

155 clamps in the presence of a non-hydrolyzable ATP analog compared with the wild type enzyme.

156 e of magnesium and ATP (or a nonhydrolyzable ATP analog), contains maximal DNA helicase in the presen

157 with the models based on the nonhydrolyzable ATP analog data.

158 (fluorosulfonyl)benzoyl]adenosine (FSBA), an ATP analog, demonstrate that both inhibitors bind to the

159 CW domains in complex with a nonhydrolyzable ATP analog demonstrates that the two domains are directl

160 Non-hydrolyzable ATP analogs did not substitute for ATP in the RNA-unwind

161 Nonhydrolyzable ATP analogs did not substitute for ATP to promote recove

162 ATP-gamma-S, a poorly hydrolyzable ATP analog, did not support endocytosis but instead prod

163 wever, in the presence of a non-hydrolyzable ATP analog, DNA binding was only slightly compromised.

164 rom Dictyostelium in the presence of various ATP analogs do not show changes at the reactive thiol re

165 midodiphosphate (AMP-PNP), a nonhydrolyzable ATP analog, each kinesin-1 dimer binds two tubulin heter

166 The accessibility of the fluorescent ATP analog, epsilon ADP, to acrylamide quenching was als

167 pecific interactions with the adenine of the ATP analog, establishing the molecular basis of ATP reco

168 Substitution of nonhydrolyzable ATP analogs for ATP slowed or prevented recovery.

169 in, solved to 2.4 A both with and without an ATP analog, form isologous, but asymmetric homodimers.

170 Two ATP analogs, FSBA and ATP gamma S, used in this study, w

171 olyzable (AMP-PNP, AMP-PCP) nor hydrolyzable ATP analogs (GTP, CTP, UTP, and ITP) activated hIK1.

172 The addition of a nonhydrolyzable ATP analog had no effect at early time periods (measured

173 e in complex with a peptide substrate and an ATP analog has been determined at 1.9 A resolution.

174 structure of this mutant in complex with an ATP analog has been determined at 2.4-A resolution.

175 kinase domain and its binary complex with an ATP analog has revealed an identical open kinase conform

176 o]triphosphate (AMP-PNP), a non-hydrolyzable ATP analog, has no effect on MGAD activity.

177 iphosphate (AMP-PNP), a hydrolysis-resistant ATP analog; however, this study mainly used AMP-PNP to f

178 iotic phosphate transfer system involving an ATP analog (imidazole phosphate) and histidyl peptides,

179 -type Cdk7 with a version sensitive to bulky ATP analogs in human cancer cells.

180 Inclusion of ATP analogs in the binding assay with Ca2+ and Mg2+ to s

181 ormation when complexed with nonhydrolysable ATP analogs, in contrast to other transporter structures

182 A series of ATP analogs, in which moieties of various sizes have bee

183 presence of either ADP or a non-hydrolyzable ATP analog induces conversion to a monomeric form.

184 Nonhydrolyzable or poorly hydrolyzable ATP analogs inhibited MgATP-supported binding.

185 e specifically sensitive to a cell-permeable ATP analog inhibitor, allowing us to perform high-resolu

186 transduction networks has relied heavily on ATP analog inhibitors.

187 difications that confer sensitivity to novel ATP analog inhibitors.

188 tibility of another protein kinase, PDK1, to ATP analog inhibitors.

189 Specifically, ATPgammaS (a nonhydrolyzable ATP analog) inhibits secretion of interferon gamma by NK

190 dominantly the P2X7 receptor (P2X7R), via an ATP analog initiate innate proinflammatory inflammation,

191 Binding of a nonhydrolyzable ATP analog inverts the transporter to an outward-facing

192 synchronous meiosis at 25 degrees C when an ATP analog is added to the culture.

193 tegy, a neo-substrate approach involving the ATP analog kinetin triphosphate (KTP), can be used to in

194 Binding of a nonhydrolyzable ATP analog locks pNS3h in a conformation that is more co

195 The fluorescent ATP analog, mantATP (2'(3')-O-(N-methylanthraniloyl)ATP)

196 Base-modified ATP analogs may exert their biological effects through p

197 ce microscope, we found that the fluorescent ATP analog methylanthraniloyl ATP (mantATP), which has b

198 The MDE structure with an ATP analog (MgADP x BeFx) was also determined to 3.6 A r

199 Previously we found that the ATP analog N(6)-(2-phenylethyl)-ATP (P-ATP) potentiates

200 PKG Ialpha (M438G) that efficiently used the ATP analog N(6)-benzyl-ATP.

201 nase displayed catalytic efficiency with the ATP analog, N(6)-(cyclopentyl) ATP, which is similar to

202 Interestingly, we found that the ATP analog N6-(2-phenylethyl)-ATP (P-ATP) increases G551

203 eport the PAICS structure with SAICAR and an ATP analog occupying the SAICAR synthetase active site.

204 The effects of modified ATP analogs on ATP-dependent poly(A) tail synthesis by y

205 finity for ATP, to probe the action of these ATP analogs on conformational switching.

206 Effects of two nonhydrolyzable ATP analogs on helicase denaturation were measured as co

207 the effects of ATP, ADP, and nonhydrolyzable ATP analogs on the lifetime of protein.DNA complexes.

208 In the presence of an ATP analog only one conformation is observed, indicating

209 ocked by the use of either a nonhydrolyzable ATP analog or a single-ring GroEL mutant, substrates com

210 ection from inhibition by a non-hydrolyzable ATP analog or acetylphosphate, in conjunction with the s

211 mplex with either APS and a non-hydrolyzable ATP analog or APS and sulfate revealed the overall struc

212 e enzyme is titrated with a non-hydrolyzable ATP analog or the enzyme is mutated such that it is able

213 hree structures of human ATP13A2 bound to an ATP analog or to spermine in the presence of phosphomime

214 n the presence of AMP-PNP (an unhydrolyzable ATP analog) or the autophosphorylation-site mutant, T267

215 ciated with ATPgammaS, a poorly hydrolyzable ATP analog, or ADP plus AlF(4), which mimics the transit

216 S, a nonphysiological and slowly hydrolyzed ATP analog, or by inactivating one of the two nucleotide

217 er with ATP-gamma-s, the slowly hydrolyzable ATP analog, or with ATP in the presence of alpha, beta-m

218 as observed with MgADP, with nonhydrolyzable ATP analogs, or with MgATP by catalytically inactive eny

219 diphosphate (AMPPNP), a hydrolysis-resistant ATP analog, prior to treatment with FSBMantAdo resulted

220 imidodiphosphate (AMPPNP), a nonhydrolyzable ATP analog, promotes stable complex formation between Re

221 bound to labeled RNA and a non-hydrolyzable ATP analog provide a direct view of how large domain mov

222 Photocrosslinking ATP analogs provide powerful tools to study kinases by c

223 treatment with ATPgammaS, a nonhydrolyzable ATP analog, recapitulated early signaling events associa

224 tified using a combination of more selective ATP analogs, receptor expression studies, and study of d

225 The three complexes of ABCB10/ATP analogs reported here showed varying degrees of open

226 eta,gamma-imidoadenosine 5'-triphosphate, an ATP analog, resolution 3.1 angstrom), ADP-P(i) (ADP with

227 versible in the presence of non-hydrolysable ATP analogs, resulting in a strong hyperpolarizing shift

228 omplex in the presence of a non-hydrolysable ATP analog reveal how nucleotide binding primes the comp

229 stal structure in the presence of Ca(2+) and ATP analog reveals a rotated headpiece, altered connecti

230 ty (5-8-fold) and binding of the 14C-labeled ATP analog rho-fluorosulfonylbenzoyl 5'-adenosine (FSBA)

231 The Prp43*ATP-analog*RNA complex shows the localization of the RNA

232 ed the crystal structure of the human eIF4A1.ATP analog.RocA.polypurine RNA complex.

233 not ATP hydrolysis, because nonhydrolyzable ATP analogs satisfy the nucleotide requirement.

234 horylation in receptor activation, and 4) an ATP analog selectively inhibits the GC-B mutants, indica

235 ntage of 3 different small molecules and the ATP-analog sensitive kinase allele technology to interce

236 Inhibition of an ATP analog-sensitive allele of Cdk1 completely blocked t

237 To explore Ypk1/2 function, we constructed ATP analog-sensitive alleles of both kinases and monitor

238 Using ATP analog-sensitive alleles of PKA and Sch9, we find th

239 nhibition of PKA catalytic subunits that are ATP analog-sensitive causes increased Bcy1 phosphorylati

240 Here we use an ATP analog-sensitive form of ATM to determine that ATP b

241 Chemical inactivation of an ATP analog-sensitive form of the Pat1 kinase (pat1-as2)

242 Moreover, using an ATP analog-sensitive PKCdelta mutant in mouse L(tk(-)) f

243 Here we developed an ATP analog-sensitive PKCepsilon mutant to selectively in

244 3.8 A resolution) for this S1 complexed with ATP analogs, some of which are cross-linked by para-phen

245 Using the engineered kinases and an ATP analog, specific kinase substrates within the PIC we

246 normal function and disease, we developed an ATP analog-specific (AS) PKCdelta that is sensitive to s

247 -(benzyl) ATP to a cell lysate containing an ATP analog-specific kinase allele (as1 allele) results i

248 Finally, a photoactivable ATP analog specifically labeled presenilin 1-C-terminal

249 ret disjunctional protein) motors in ATP and ATP-analog states.

250 el is partly based on the reduced ability of ATP analogs such as adenosine 5'-(beta,gamma-imino)triph

251 ATP analogs such as ADP and AMP exerted marked inhibitor

252 Finally, experiments with non-hydrolyzable ATP analogs suggest that SpoIIIE can operate with non-co

253 netic and binding studies with a fluorescent ATP analog suggested that ATP induces a conformational c

254 ase, and binding analyses with a fluorescent ATP analog suggested unequal contributions by the two nu

255 inhibited by the addition of nonhydrolyzable ATP analogs, suggesting that ATP hydrolysis and not just

256 Finally, sensitivity to various ATP analogs suggests that all editing-like activities re

257 magnitude weaker than in the presence of the ATP analog (tense state).

258 Cdk1 was engineered to accept an ATP analog that allows it to uniquely label its substrat

259 Slippage is not reduced by an ATP analog that blocks promoter escape, but it is inhibi

260 we show using two independent approaches--an ATP analog that can drive CFTR channel gating but is uns

261 nalysis the protective effect of AMP-PCP, an ATP analog that is not utilized for enzyme phosphorylati

262 s to be uniquely sensitive to inhibitors and ATP analogs that are not recognized by wild-type kinases

263 Searching for ATP analogs that strongly bound to Thermus aquaticus (Ta

264 ns (unlike with assays utilizing radioactive ATP analogs), the assay described can be used to disting

265 ly, whereas with AMP-PNP, a non-hydrolyzable ATP analog, the affinity was 11 nM.

266 Upon binding the ATP analog, the DnaB hexamer transforms into a "tense" s

267 In the presence of a nonhydrolyzable ATP analog, the enzyme is known to promote a single turn

268 In the absence of the ATP analog, the hexamer exists in a "closed" conformatio

269 In the presence of ATP or a nonhydrolyzable ATP analog, the initial step is the self-assembly of Clp

270 Despite being in complex with an ATP analog, the kinase domain of GRK6 remains in an open

271 For several ATP analogs, the concentration required to inhibit P2X3

272 crystal structures in complexes with RNA and ATP analogs, Thomsen and Berger now elucidate the molecu

273 troms resolution and in complex with with an ATP analog to 2.3 angstrosms A resolution.

274 but not the wild type (WT) kinase, used the ATP analog to phosphorylate both a model peptide substra

275 Second, the binding of a nonhydrolyzable ATP analog to the yeast enzyme appears to affect citraco

276 igases and also point to strategies that use ATP analogs to improve specificity.

277 tic resonance spectroscopy with spin-labeled ATP analogs to probe the structure of the ATP active sit

278 e kinetics of binding mantATP (a fluorescent ATP analog) to the microtubule K341 complex, the dissoci

279 The finding that slowly hydrolyzable ATP analogs trigger slower nucleotide release than ATP s

280 pool also can be filled with the fluorescent ATP analog trinitrophenol ATP, as well as with a photoac

281 The 6-histidine loop bound the fluorescent ATP analog trinitrophenyl-ATP with high affinity, as det

282 bstrate, as well as spectroscopically active ATP analogs (trinitrophenyl-ATP and ATP gamma S-acetamid

283 circle in the presence of a nonhydrolyzable ATP analog under the same conditions that the wild type

284 rent activation in the presence of ATP or an ATP analog unsuitable for phosphotransfer, as well as ps

285 bstrate on which RecA is polymerized and the ATP analog used.

286 engineered to accept bulky N(6)-substituted ATP analogs, using a chemical genetics approach initiall

287 A non-hydrolyzable ATP analog was a competitive inhibitor.

288 ase kinase/phosphatase in the presence of an ATP analog was attempted.

289 However, the N(6)-(cyclopentyl) ATP analog was not accepted by the wild-type kinase.

290 (yPAP) toward various C-2- and C-8-modified ATP analogs was examined.

291 Importantly, the effect of these ATP analogs was prevented in the presence of intracellul

292 -actin with bound AMPPNP, a non-hydrolyzable ATP analog, was determined to 1.85-A resolution.

293 Upon removal of an ATP analog, we show that the nucleotide-binding pocket i

294 l-regulated kinase 2 (ERK2) that can utilize ATP analogs, we have identified the alternative mRNA spl

295 ix/chemical quench experiments using various ATP analogs were performed.

296 tructures of yeast dynein bound to different ATP analogs, which collectively provide insight into the

297 g by BLM in the presence of non-hydrolysable ATP analogs, which has implications for the underlying m

298 re fully bound to adenosine 5'-triphosphate (ATP) analogs, which is expected to induce opening of the

299 ased on their ability to bind a spin-labeled ATP analog with stoichiometries and equilibrium binding

300 erently in the presence of a nonhydrolyzable ATP analog, with subconductance openings significantly s