ライフサイエンスコーパス: Aquifex

1 d on the stem structures associated with the Aquifex 16S and 23S rRNA precursors are cleaved at sites

2 formed in vitro cleavage of dsRNAs by Ec and Aquifex aeolicus (Aa) enzymes and delineated their produ

3 ss I BPL from the hyperthermophilic bacteria Aquifex aeolicus (AaBPL) in its ligand-free form and in

4 rom the hyperthermophilic, ancient bacterium Aquifex aeolicus (Aacpn10) has a 25-residue C-terminal e

5 deep-branching, hyperthermophilic bacterium Aquifex aeolicus (Aacpn10) shows high homology with meso

6 atured cpn10 from Homo sapiens (hmcpn10) and Aquifex aeolicus (Aacpn10) were monitored by far-UV circ

7 meric co-chaperonin proteins 10 (cpn10) from Aquifex aeolicus (Aacpn10-del25) and human mitochondria

8 al structure of the leucine transporter from Aquifex aeolicus (aaLeuT) has provided significant insig

9 end, we investigated a thermostable LS from Aquifex aeolicus (AaLS) and found that it also forms cag

10 empted to convert the capsid-forming LS from Aquifex aeolicus (AaLS) into pentamers through a small n

11 l, 60-subunit capsid, lumazine synthase from Aquifex aeolicus (AaLS), to act as a container for nucle

12 chaeum equitans, Pyrobaculum aerophilum, and Aquifex aeolicus (all hyperthermophiles).

13 ral gene fliA was exchanged with homologs of Aquifex aeolicus (an extreme thermophile) and Chlamydia

14 The ribosomal protein S8 from Aquifex aeolicus (AS8) is unique in that there is a 41-r

15 al structure of the leucine transporter from Aquifex aeolicus (LeuT).

16 e present the crystal structure of MraY from Aquifex aeolicus (MraYAA) at 3.3 A resolution, which all

17 e present the crystal structure of MraY from Aquifex aeolicus (MraYAA) in complex with its naturally

18 Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphat

19 Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphat

20 The [2Fe-2S] ferredoxin (Fd4) from Aquifex aeolicus adopts a thioredoxin-like polypeptide f

21 We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and

22 CAK dynamics with those of hyperthermophilic Aquifex aeolicus AK (AAAK), our results provide strong e

23 crystal structures of an active fragment of Aquifex aeolicus alanyl-tRNA synthetase complexed, separ

24 crystal structure of a catalytic fragment of Aquifex aeolicus AlaRS and additional data suggest how t

25 of the 453 amino acid catalytic fragment of Aquifex aeolicus AlaRS.

26 teases from two different bacterial species, Aquifex aeolicus and B. subtilis.

27 (there termed PRORPs) and in some bacteria (Aquifex aeolicus and close relatives); both enzyme types

28 ed the MpgII genes from T. maritima and from Aquifex aeolicus and found that both genes could restore

29 similar studies done with SPS orthologs from Aquifex aeolicus and humans, we propose a catalytic mech

30 The amt genes of the hyperthermophiles Aquifex aeolicus and Methanococcus jannaschii complement

31 le for two different PGT domains, PBP1A from Aquifex aeolicus and PBP1A from Escherichia coli.

32 ture of NusB from the thermophilic bacterium Aquifex aeolicus and studied the interaction of NusB and

33 rt the kinetic characterization of LpxK from Aquifex aeolicus and the crystal structures of LpxK in c

34 terized aldolases of Helicobacter pylori and Aquifex aeolicus and to the group that comprises the Cal

35 Escherichia coli and the metallo KDO8PS from Aquifex aeolicus are the best characterized members of e

36 e of the full-length WzmWzt transporter from Aquifex aeolicus bound to adenosine triphosphate (ATP) a

37 d of CTP and ATP; we transformed the related Aquifex aeolicus CC- and A-adding enzymes into UU- and G

38 KDO8PS) from the hyperthermophilic bacterium Aquifex aeolicus differs from its Escherichia coli count

39 was used to probe conformational changes of Aquifex aeolicus dihydroorotase (DHO), which catalyzes t

40 The X-ray structures of Aquifex aeolicus dihydroorotase in two space groups, C22

41 solution that the ATP-dependent assembly of Aquifex aeolicus DnaA into a spiral oligomer creates a c

42 d the structure of the conserved core of the Aquifex aeolicus DnaA protein to 2.7 A resolution.

43 Based on the structural similarity of Aquifex aeolicus DnaA to other AAA+ proteins that are ol

44 deled upon the crystallographic structure of Aquifex aeolicus DnaA, predicts a hydrogen bond between

45 A 3.3 A crystal structure of Aquifex aeolicus DnaB, complexed with nucleotide, reveal

46 The genome of the bacterium Aquifex aeolicus encodes a polypeptide which is related

47 The phoU gene of Aquifex aeolicus encodes a protein called PHOU_AQUAE wit

48 The deeply rooted organism Aquifex aeolicus encodes one type IIA topoisomerase conf

49 ly homologous and structurally characterized Aquifex aeolicus ferredoxin 4 (AaeFd4) using EPR, UV-vis

50 o-EM to determine 3D maps of the full-length Aquifex aeolicus FtsH protease.

51 We determined the cryo-EM structure of Aquifex aeolicus HARP (Aq880) and two crystal structures

52 The hyperthermophilic bacterium Aquifex aeolicus has a MutL protein (Aae MutL) that poss

53 conserved hypothetical protein, Aq1575, from Aquifex aeolicus has been determined by using x-ray crys

54 ecent NMR and X-ray studies of the LpxC from Aquifex aeolicus have provided the first structural info

55 tulosonate 8-phosphate (KDO8P) synthase from Aquifex aeolicus in complex with phosphoenolpyruvate (PE

56 The placement of the extreme thermophile Aquifex aeolicus in the bacterial phylogenetic tree has

57 of the alpha2beta2 GlyRS from the bacterium Aquifex aeolicus is able to perform the first step of th

58 of unknown function family 507 protein from Aquifex aeolicus is reported (AaDUF507, UniProt O67633,

59 have investigated the mechanism of action of Aquifex aeolicus IspH [E-4-hydroxy-3-methyl-but-2-enyl d

60 We report the inhibition of the Aquifex aeolicus IspH enzyme (LytB, (E)-4-hydroxy-3-meth

61 bstrate permeation pathway in the homologous Aquifex aeolicus leucine transporter.

62 us ( Tth ) ligase, Thermus sp. AK16D ligase, Aquifex aeolicus ligase and the K294R mutant of the Tth

63 h amino acid was altered in both E. coli and Aquifex aeolicus LpxC and the catalytic activities of th

64 k(cat)/Km catalyzed by Escherichia coli and Aquifex aeolicus LpxC displayed a bell-shaped curve (EcL

65 h the wild type (WT) and the H265A mutant of Aquifex aeolicus LpxC, each in the absence of substrate

66 ctures of apo- and ADP/Mg(2+)-bound forms of Aquifex aeolicus LpxK to a resolution of 1.9 A and 2.2 A

67 ling nanoparticles, including the 60-subunit Aquifex aeolicus lumazine synthase (LuS) and the 24-subu

68 ared and studied, His42, His124, and Glu126 (Aquifex aeolicus numbering), with particular attention p

69 Relative to EcNusG, Aquifex aeolicus NusG (AaNusG) and several other bacteri

70 doglycan glycosyltransferase (PGT) domain of Aquifex aeolicus PBP1A.

71 4Fe-3S] cluster in hydrogenase (Hase) I from Aquifex aeolicus performs two redox transitions within a

72 When introduced into purified Aquifex aeolicus PilT, substitutions in the AIRNLIRE mot

73 D structure of the central domain from NtrC1 Aquifex aeolicus protein into our 3D model; we propose t

74 and genetic approaches that CCA addition in Aquifex aeolicus requires collaboration between two rela

75 gnetic resonance (NMR) analysis of SmpB from Aquifex aeolicus revealed an antiparallel beta-barrel st

76 [2Fe-2S] cluster containing ferredoxin from Aquifex aeolicus reveals a thioredoxin-like fold that is

77 crystal structure of the nuclease domain of Aquifex aeolicus RNase III, the E41, D114, and E117 side

78 Here, we present two crystal structures of Aquifex aeolicus SD, including a ternary complex with bo

79 previously determined crystal structures of Aquifex aeolicus SelA complexed with tRNA(Sec) revealed

80 tal structures of GAF regulatory domains for Aquifex aeolicus sigma(54) activators NifA-like homolog

81 We identified a minimal construct of the Aquifex aeolicus sigma(54) AID that consists of two pred

82 We report here the structure of an Aquifex aeolicus sigma(54) domain (residues 69-198), whi

83 e same affinity for the Escherichia coli and Aquifex aeolicus SmpB proteins as the intact E. coli tmR

84 Trbp111 is a 111 amino acid Aquifex aeolicus structure-specific tRNA-binding protein

85 protein from the hyperthermophilic bacterium Aquifex aeolicus suggested that this protein functions s

86 mily members, we determined the structure of Aquifex aeolicus ThiL (AaThiL) with nonhydrolyzable AMP-

87 n of the bacterial helicase loader DnaC from Aquifex aeolicus to 2.7 A resolution.

88 eport a 2.6 angstrom co-crystal structure of Aquifex aeolicus Trbp111 bound to tRNA(Ile), which revea

89 ligase from the hyperthermophilic bacterium Aquifex aeolicus was cloned, expressed in Escherichia co

90 homologue from the thermophilic eubacterium Aquifex aeolicus was cloned, overexpressed, and purified

91 Aquifex aeolicus was one of the earliest diverging, and

92 namide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and

93 activity-based screen, two phosphatases from Aquifex aeolicus were identified that dephosphorylate AR

94 fashion, whereas the Class II enzymes (e.g. Aquifex aeolicus) require metal ions for catalysis.

95 or associate into multifunctional complexes (Aquifex aeolicus).

96 tion structure of a leucine transporter from Aquifex aeolicus, a bacterial member of the SLC6 transpo

97 out the active site environment of LpxC from Aquifex aeolicus, a heat-stable orthologue that displays

98 The enzyme from Aquifex aeolicus, a hyperthermophilic organism of ancien

99 -bound forms of the DBD of NtrC4 (4DBD) from Aquifex aeolicus, a member of the NtrC family of sigma(5

100 of the free and CMP-bound forms of WaaA from Aquifex aeolicus, an ancient Gram-negative hyperthermoph

101 Aquifex aeolicus, an extreme hyperthermophile, has neith

102 of intact NtrC4 (a sigma(54) activator from Aquifex aeolicus, an extreme thermophile), as well as it

103 Aquifex aeolicus, an organism that flourishes at 95 degr

104 n from Escherichia coli, a KtrB protein from Aquifex aeolicus, and a Trk1,2 protein from Schizosaccha

105 studies of NtrC4, a sigma-54 activator from Aquifex aeolicus, and compare it with NtrC1 (a paralog)

106 e LpxC deacetylase from the hyperthermophile Aquifex aeolicus, and it has excellent antibiotic activi

107 L27, was cloned from the extreme thermophile Aquifex aeolicus, and the protein was overexpressed and

108 erichia coli, Agrobacterium tumefaciens, and Aquifex aeolicus, as well as the ADAT2-ADAT3 proteins fr

109 charomyces cerevisiae, and from the bacteria Aquifex aeolicus, Borrelia burgdorferi, Clostridium stic

110 A hemoglobin was identified in Aquifex aeolicus, cloned, recombinantly expressed, purif

111 GatCAB from the hyperthermophilic bacterium Aquifex aeolicus, complexed with glutamine, asparagine,

112 The thermophilic chemolithotroph, Aquifex aeolicus, expresses a gene product that exhibits

113 nanomolar concentrations, including that of Aquifex aeolicus, for which structural information is av

114 e E/G homologs from phylogenetically distant Aquifex aeolicus, Haemophilus influenzae Rd, and Synecho

115 mains from heterologous organisms, including Aquifex aeolicus, localized to septal rings when produce

116 Here, we demonstrate that KDO8PS from Aquifex aeolicus, representing only the second member of

117 enzymes from human, Myxococcus xanthus, and Aquifex aeolicus, respectively.

118 mologue from the hyperthermophilic bacterium Aquifex aeolicus, that shares 35.2% identity with human

119 Given the ancient lineage of Aquifex aeolicus, this observation provides the first in

120 and ATP; however, we recently found that in Aquifex aeolicus, which lies near the deepest root of th

121 we report the crystal structure of LpxC from Aquifex aeolicus, which reveals a new alpha+beta fold re

122 , including one from the extreme thermophile Aquifex aeolicus, which suggests that RusA may be of anc

123 avorably with the -tolerant hydrogenase from Aquifex aeolicus, which we use here as a benchmark.

124 re, we present new structures of FtsZ from47 Aquifex aeolicus,47 Bacillus subtilis, Methanococcus jan

125 e NtrC1 protein from the extreme thermophile Aquifex aeolicus.

126 tG6PDH) from the hyperthermophilic bacterium Aquifex aeolicus.

127 Delta 67) from the extreme hyperthermophile Aquifex aeolicus.

128 he hyperthermophiles Thermotoga maritima and Aquifex aeolicus.

129 cation apparatus of the extreme thermophile, Aquifex aeolicus.

130 hromosome of the hyperthermophilic bacterium Aquifex aeolicus.

131 roteins from the hyperthermophilic bacterium Aquifex aeolicus.

132 and purified LpxC from the hyperthermophile Aquifex aeolicus.

133 e traffic in the hyperthermophilic bacterium Aquifex aeolicus.

134 mbranes of the hyperthermophilic eubacterium Aquifex aeolicus.

135 of TatC from the hyperthermophilic bacterium Aquifex aeolicus.

136 enzyme from the hyperthermophilic bacterium, Aquifex aeolicus.

137 e of the leucine transporter (LeuT(Aa)) from Aquifex aeolicus.

138 ure of the C-terminal domain of sigma54 from Aquifex aeolicus.

139 y RNase P in the hyperthermophilic bacterium Aquifex aeolicus: Without an RNA subunit and the smalles

140 Despite low expression levels, His-tagged Aquifex Amt could be purified by heating and nickel chel

141 ic analysis of ribosomal proteins identifies Aquifex as grouping with Thermotoga another bacterial hy

142 Comparison of the Aquifex ATP sulfurylase active site with those from sulf

143 2.3 A resolution X-ray crystal structure of Aquifex ATP sulfurylase-APS kinase bifunctional enzyme i

144 s of Group I, which includes Proteobacteria, Aquifex, Chlamydia, Spirochaetes, Cytophaga-Chlorobium,

145 al HJRs in low-GC Gram-positive bacteria and AQUIFEX: Endonuclease VII of phage T4 is shown to serve

146 The thermal stability of the Aquifex enzyme can be explained by the 43% decreased cav

147 The domain arrangement in the Aquifex enzyme is reminiscent of the fungal ATP sulfuryl

148 Therefore, the Aquifex enzyme may represent an ancestral homolog of a p

149 th previous studies on Thermus ligases, this Aquifex ligase exhibits greater discrimination against a

150 ative polyA polymerases in Synechocystis and Aquifex, PRUNE of Drosophila, and an exopolyphosphatase

151 erall stereochemistry of the metal-dependent Aquifex pyrophilus KDO8P synthase (ApKDO8PS) reaction wa

152 ucture, but differing substantially from the Aquifex pyrophilus RacE structure.

153 of MurI from the hyperthermophilic bacterium Aquifex pyrophilus, we performed molecular dynamics (MD)

154 Homologs of Aquifex RNase P (HARP) were identified in many Archaea a

155 bacterial/archaeal variant termed homolog of Aquifex RNase P (HARP); the latter replaced the RNA-base

156 Homologs of Aquifex RNase P (HARPs) are also expressed in some other

157 overed class of RNase P enzymes, Homologs of Aquifex RNase P (HARPs).

158 s, Bartonella, Nitrosomonas, Thermotoga, and Aquifex showed a strong preference for L-ornithine, wher

159 e played a role in several lineages, such as Aquifex, Thermotoga, and Fusobacterium.

160 deviation from this evolutionary pattern is Aquifex whose DdRp subunits cluster within Group I, wher