ライフサイエンスコーパス: Cas9

1 made by programmable nucleases (e.g. CRISPR-Cas9).

2 n (FACT) as an interactor of substrate-bound Cas9.

3 he MT1-MMP gene was knocked out using CRISPR/Cas9.

4 it also produced indels when delivered with Cas9.

5 cluding FOXO1, which we confirm using CRISPR/Cas9.

6 e DNA recognition lobe in full-length CRISPR-Cas9.

7 ll, targeted knockout (KO)-first, and CRISPR/Cas9.

8 -like effector nucleases (TALENs) and CRISPR-Cas9.

9 te during editing with Cascade-Cas3, but not Cas9.

10 either floxed Th mice or viral-based CRISPR/Cas9.

11 enerated a Prcd-KO animal model using CRISPR/Cas9.

12 genetic inactivation of KDM6A/B using CRISPR/Cas9.

13 ng a catalytically inactive Adar with CRISPR/Cas9.

14 Here, we describe an optimized Cas9-AAV6-based genome editing tool platform for site-sp

15 atient skin samples and an engineered CRISPR/Cas9 ABCA12 KO cell line.

16 Recently, second-generation CRISPR/Cas9 activation systems based on nuclease inactive dead

17 r tunable and reversible control over CRISPR-Cas9 activity.

18 induced by Cas9_Trex2, as opposed to 3.5% by Cas9 alone, were repaired through microhomology-mediated

19 We compared the performance of Cas9 and Cas12a enhanced E-DNA sensor and optimized the

20 However, the leading Cas9 and Cas12a enzymes are limited in their ability to

21 concerns by splitting the drive components, Cas9 and gRNAs, into separate alleles to form a trans-co

22 Here, using a combination of CRISPR-Cas9 and transposon technologies, we show that pigs with

23 pha in response to genetic (shRNA and CRISPR/Cas9) and pharmacologic (crizotinib) inhibition of c-MET

24 paced short palindromic repeats-Cas9 (CRISPR-Cas9) and transposon vectors to disrupt Trp53 and overex

25 e3 methyltransferase complex by using CRISPR/Cas9, and obtained three deletion mutants.

26 ally using targeted nucleases such as CRISPR/Cas9, and suppression of gene expression, typically usin

27 ditional deletions in mice as well as CRISPR/Cas9 approaches to target CTNND1 in Xenopus, we identifi

28 or improving the specificity and kinetics of Cas9 as a genome engineering tool and may inspire expand

29 CRISPR-Cas9-associated base editing is a promising tool to corr

30 ogies, single-cell RNA-sequencing and CRISPR-Cas9 barcode editing for elucidating developmental linea

31 Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal

32 dicot Arabidopsis Here, we employed a CRISPR/Cas9-based approach to disrupt a subset of cytokinin his

33 Here, we describe CRISPR/Cas9-based editing of exon 1 of the HVT079 and HVT096 ge

34 Using a CRISPR/Cas9-based engineering approach, we genetically deleted

35 Here we perform a CRISPR/Cas9-based functional screening of 59 membrane proteins

36 Using a CRISPR/Cas9-based gene knockout approach, we identified galecti

37 CRISPR/Cas9-based gene knockout in animal cells, particularly i

38 e utilized a single-step method where CRISPR/Cas9-based gene knockout is combined with insertion of a

39 ression, we perform unbiased RNAi and CRISPR-Cas9-based genetic screens in vivo.

40 To realize the promise of CRISPR-Cas9-based genetics, approaches are needed to quantify a

41 Here we show that a CRISPR/Cas9-based genome editing strategy allows the precise co

42 of the identified rare variants using CRISPR/Cas9-based knock-in human dopaminergic SH-SY5Y cell line

43 RNAs for alternative exon removal), a CRISPR-Cas9-based method to manipulate isoforms independent of

44 us Mov10 promotes HCV replication, as CRISPR-Cas9-based Mov10 depletion decreases HCV replication and

45 We developed an image- and CRISPR/Cas9-based pipeline to systematically characterize candi

46 Here we used CRISPR-Cas9-based screening to search for additional RQC strate

47 their widespread adoption has lagged behind Cas9-based strategies due to low activity and the lack o

48 We developed a CRISPR/Cas9-based system to express catalytically dead PfClpP,

49 determined this arrest is driven in part by Cas9 binding to DNA.

50 er adjacent motif (PAM) requirements, CRISPR/Cas9 cannot access many genetic loci.

51 formation of resistance alleles that prevent Cas9 cleavage.

52 tudy, we used the RNA-guided nuclease CRISPR-Cas9 (clustered regularly-interspaced short palindromic

53 high-throughput screening of shRNA or CRISPR-Cas9 constructs to identify genes that regulate human ma

54 es on both the termini and internal sites of Cas9, creating a platform for endowing Cas9 with diverse

55 ularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) and transposon vectors to disrupt Trp

56 nation of the DNA repair outcome following a Cas9 cutting.

57 Here, we use deactivated Cas9 (dCas9) and deactivated Cas12a (dCas12a) to constru

58 Using the CRISPR/nuclease-deactivated Cas9 (dCas9)-based CARRY (CRISPR-assisted RNA-RNA-bindin

59 uring lysogeny in Listeria, AcrIIA1 triggers Cas9 degradation.

60 A modest increase in Cas9-dependent and -independent DNA off-target editing,

61 as tested using a specially developed CRISPR/Cas9 DNA damage induction system, capable of inducing sm

62 c infection, however, AcrIIA1 fails to block Cas9 due to its multi-step inactivation mechanism.

63 However, CRISPR/Cas9 edited F(0) animals too often demonstrate variable

64 In a population of these CRISPR-Cas9 edited plants (n = 780) that was phenotyped for tas

65 Experiments with CRISPR-Cas9-edited sorghum further indicate that the benefit of

66 CRISPR-Cas9-edited zebrafish were used as an in vivo model to a

67 MIHR mutant jellyfish generated using CRISPR-Cas9 editing had severe defects in gamete development or

68 leles of the NANOS2 gene generated by CRISPR-Cas9 editing have testes that are germline ablated but o

69 terspaced short palindromic repeats (CRISPR)-Cas9 editing of immune checkpoint genes could improve th

70 Using CRISPR/Cas9 editing to endogenously tag receptors with fluoresc

71 n mice, dogs, and human cells through CRISPR/Cas9 editing.

72 Gene editing nuclease represented by Cas9 efficiently generates DNA double strand breaks at t

73 string (the CRISPR guide RNA) can guide the Cas9 endonuclease to specific locations in complex genom

74 A corresponding CRISPR/Cas9 engineered mouse model recapitulated both the clini

75 successful intracellular delivery of CRISPR/Cas9, especially in the form of ribonucleoprotein (RNP),

76 d by electroporation of embryos derived from Cas9-expressing donor females.

77 AL4 enhancer-trap lines into tissue-specific Cas9-expressing lines.

78 -Cas9 transgenes, which allow fine tuning of Cas9 expression to achieve high gene editing activity wi

79 the targeting mechanism specified by CRISPR/Cas9 forces integration into genomic regions that are ot

80 IA1 also uniquely inhibits a highly diverged Cas9 found in Listeria (similar to SauCas9) and Type II-

81 ltiple expression-based phenotypes in CRISPR/Cas9 functional screening that uses single-cell RNA-seq

82 epeats (CRISPR)-CRISPR-associated protein 9 (Cas9) functional analyses in Parhyale, we show that a ge

83 n systems based on nuclease inactive dead (d)Cas9 fused to transcriptional transactivation domains we

84 Using a CRISPR-Cas9 gene activation approach, we showed that the expres

85 mycin, inducible conditional mice, or CRISPR/Cas9 gene editing decreased cell migration due to the lo

86 The present CRISPR/Cas9 gene editing dogma for single guide RNA (sgRNA) del

87 ve and cell-type-specific delivery of CRISPR/Cas9 gene editing elements remains a challenging open pr

88 The clinical application of CRISPR-Cas9 gene editing has been eagerly awaited since the fir

89 Proteomic analysis and CRISPR/Cas9 gene editing identified the inflammatory glycoprote

90 Furthermore, our highly efficient CRISPR/Cas9 gene editing in primordial germ cells represents a

91 viral resistance conferred by precise CRISPR/Cas9 gene editing in the chicken.

92 ar approaches can be used to forecast CRISPR/Cas9 gene editing outcomes in Xenopus tropicalis, Xenopu

93 Employing CRISPR/Cas9 gene editing to disrupt the Pdgfra gene in two diff

94 ic mutations in Arabidopsis At2OGO by CRISPR/Cas9 gene editing.

95 gene (FAF1) in DLD-1 CRC cells using CRISPR/Cas9 gene editing; some cells were transfected with plas

96 andidate tumor suppressors we applied CRISPR/Cas9 gene inactivation screens to a cellular model of ea

97 conclude that clinical application of CRISPR-Cas9 gene-edited T cells is generally safe and feasible.

98 r, these results demonstrate that the CRISPR-Cas9 generated Gaa(c.1826dupA) murine model recapitulate

99 Here, we characterized CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants and found th

100 Using CRISPR-Cas9-generated hdac1- or hdac2-null fibroblasts, we dete

101 ous expression assays and analyses of CRISPR/Cas9-generated knockout plants.

102 uantitative complementation test with CRISPR/Cas9-generated null mutants in nonstandard wild accessio

103 A CRISPR/Cas9-generated rat model, with a 9-bp deletion within th

104 er-resolved localization analysis and CRISPR-Cas9 genetic perturbation, we find that although DAPs an

105 frontotemporal dementia patient using CRISPR/Cas9 genome editing and homology-directed repair (HDR),

106 CRISPR/Cas9 genome editing has revolutionized functional genomi

107 each predicted miRNA-binding site by CRISPR-Cas9 genome editing in C. elegans We developed a multipl

108 y combining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolated EXCESSIVE NUMBE

109 tion of marker-free DNA in rice using CRISPR-Cas9 genome editing, and offer a promising strategy for

110 level, including screens that harness CRISPR/Cas9 genome editing, natural genetic variation, proteomi

111 Using CRISPR/Cas9 genome editing, the enhancer cluster or parts there

112 With CRISPR-Cas9 genome editing, we validated Less Shattering1 (SvLe

113 CTRL-deficient strain (Ctrl-KO) using CRISPR-Cas9 genome engineering.

114 lls, three-dimensional organoids, and CRISPR-Cas9 genome-edited JEG-3 clones, we herein show that YAP

115 Here, using CRISPR Cas9 genome-wide mutagenesis to screen for genetic deter

116 Cas9 nucleases complexed with a guide RNA (Cas9-gRNA) find their targets by scanning and interrogat

117 Cas9/gRNA-mediated gene-drive systems have advanced deve

118 ises a DNA sequence-modifying enzyme such as Cas9/gRNAs that disrupts endogenous versions of an essen

119 toes do not prevent cleavage in vitro by the Cas9/guide RNA complex.

120 A Cas9/guide RNA-based gene drive strain, AgNosCd-1, was d

121 Remarkably, CRISPR/Cas9-guided single-nucleotide editing demonstrated the d

122 s and present methods for a multiplex CRISPR/Cas9 haploid screen in chimeric axolotls (MuCHaChA), whi

123 CRISPR/Cas9 has become a powerful tool for genome editing in ze

124 This study marks the first use of CRISPR/Cas9 HDR for gene integration in channel catfish and may

125 SauCas9) and Type II-C Cas9s, likely due to Cas9 HNH domain conservation.

126 In contrast to traditional CRISPR-Cas9 homology-directed repair, base editing can correct

127 repeat (CRISPR)-CRISPR associated protein 9 (Cas9) homology directed repair (HDR) to create isogenic

128 veals the absence of off-target mutations by Cas9 in the engineered plants.

129 od for measuring the genome-wide activity of Cas9 in vitro.

130 ific enhancers simultaneously using a CRISPR/Cas9 in vivo retina electroporation strategy.

131 CBE, induces a low frequency of genome-wide Cas9-independent off-target C*G-to-T*A mutation in mouse

132 high-activity CBEs while maintaining minimal Cas9-independent off-target editing.

133 e off-targets present were characteristic of Cas9-independent off-targeting and point to TC motifs as

134 Additionally, wild-type Cas9 induced fewer 53BP1 foci in TP53+/+ cells compared

135 Here, we evaluate repair outcomes of a Cas9-induced double-strand break (DSB) introduced on the

136 C terminus of dishevelled2 gene using CRISPR/Cas9-induced homologous recombination and observed its d

137 Functional validation with CRISPR-Cas9-induced mutations in novel genes Tead2, Spred1, and

138 CRISPR/Cas9 is a programmable genome editing tool widely used f

139 Cas9 is a prokaryotic RNA-guided DNA endonuclease that b

140 larly interspaced short palindromic repeats)/Cas9 is evolving rapidly.

141 CRISPR/Cas9 knockout of PTPN14 rescued keratinocyte life span e

142 CRISPR/Cas9 knockout of this enhancer induces downregulation of

143 CRISPR-Cas9 knockout of VINR in Drosophila cells enhances DCV r

144 , we describe an unbiased genome-wide CRISPR-Cas9 knockout screen that identified LPS-induced TNF-alp

145 Pooled library CRISPR/Cas9 knockout screening across hundreds of cell lines ha

146 Using pooled CRISPR-Cas9 knockout screens, we showed that teratomas can enab

147 Using CRISPR-Cas9 knockout technology, we show that these two lipid r

148 results were phenocopied with a KDM3B CRISPR/Cas9 knockout.

149 response in ES cells, we performed a CRISPR-Cas9-knockout screen.

150 We performed a CRISPR-Cas9-knockout selection designed to identify host factor

151 Homozygous deletion of ETV4, using CRISPR/Cas9, led to greatly reduced ER binding at the majority

152 sensor," tuning acr expression according to Cas9 levels.

153 , we designed a focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently publish

154 Our improved protocol incorporating a Cas9-linked selection marker and a staggered transfectio

155 CRISPR/Cas9 machinery delivered as ribonucleoprotein (RNP) to t

156 emonstrated the effective delivery of CRISPR/Cas9 machinery via zygote electroporation as an alternat

157 CRISPR/Cas9-mediated abrogation of CBFA2T3 resulted in spontane

158 Thus, we created Cas9-mediated Arrayed Mutagenesis of Individual Offsprin

159 We generated a cell culture model by CRISPR/Cas9-mediated deletion of CIB1 to study the function of

160 CRISPR/Cas9-mediated deletion of Inpp5a in the cerebellum of wi

161 Crispr-Cas9-mediated deletion of Mm47, as well as small interfe

162 CRISPR/Cas9-mediated deletion of the hypoxia-response element r

163 he Cancer Genome Atlas databases with CRISPR/Cas9-mediated depletion of the zinc finger E-box binding

164 The distance between the Cas9-mediated double-stranded break (DSB) to the mutatio

165 ouse pluripotent stem cells and using CRISPR/Cas9-mediated enrichment, determine replication rates us

166 Using CRISPR/Cas9-mediated gene editing, coupled with endocrine cell

167 any cell types have been subjected to CRISPR/Cas9-mediated gene editing, there is no evidence of succ

168 CRISPR-Cas9-mediated gene interference (CRISPRi) and activation

169 Here we establish CRISPR/Cas9-mediated genome editing in S. rosetta by engineerin

170 However, traditional CRISPR/Cas9-mediated genome editing requires plant tissue cultu

171 ad implications for the proper use of CRISPR-Cas9-mediated genome editing.

172 ls was rescued in cell lines with the CRISPR/Cas9-mediated knockout of 4E-BP1.

173 Temporally controlled CRISPR-Cas9-mediated knockouts uncovered two distinct functions

174 Here, we induced a CRISPR/Cas9-mediated KO of the TCRbeta chain in combination wit

175 CRISPR/Cas9-mediated loss of Ena/VASP proteins reduced lamellip

176 CRISPR/Cas9-mediated mutagenesis of the S. stercoralis tax-4 ge

177 CRISPR/Cas9-mediated mutation of NRC2, NRC3, and NRC4 genes did

178 Here, using CRISPR-Cas9-mediated mutations, we report that human HCT116 col

179 By contrast, CRISPR/Cas9-mediated poplar mutant nf-yb21 exhibited reduced ro

180 Further, in vivo CRISPR/Cas9-mediated REEP5 loss-of-function zebrafish mutants s

181 Here, we present a genome-wide CRISPR-Cas9-mediated screen using a human lung carcinoma cell l

182 ayed endosperm cellularization, while CRISPR-Cas9-mediated single knockout mutants showed precocious

183 lly altering memory CD8 T cells using CRISPR/Cas9-mediated targeted gene disruption under the aegis o

184 t tKO C2C12 myoblasts generated using CRISPR/Cas9 method differentiate despite the expected derepress

185 CRISPR-Cas9 methods have been applied to generate random insert

186 uide RNAs against Ube2v1 in cardiac-specific Cas9 mice alleviated CryAB(R120G)-induced protein aggreg

187 osaicism was higher in embryos injected with Cas9 mRNA (100%) compared to those injected with Cas9 pr

188 cell bovine embryos to compare the effect of Cas9 mRNA and protein on the mutation efficiency, level

189 ple gene editing techniques, including mRNA, Cas9 mRNA/single guide RNA and Cas9 ribonucleoprotein co

190 as a driver of NCC evolution, we used CRISPR-Cas9 mutagenesis(11) to disrupt edn, ednr and dlx genes

191 and colleagues perform a genome-wide CRISPR-Cas9-negative loss-of-function screen and identify WEE1

192 Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions progr

193 e editors (CGBE1), consists of an RNA-guided Cas9 nickase, an Escherichia coli-derived uracil DNA N-g

194 ormation at donor and acceptor DNA by CRISPR-Cas9 nickases (in trans paired nicking) mostly overcomes

195 targeted mutagenesis approaches like CRISPR/Cas9 now permit gene-level investigation of these mechan

196 ese findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome

197 The CRISPR-Cas9 nuclease has been widely repurposed as a molecular

198 transcribed into guide RNAs that direct the Cas9 nuclease to its target on the invader.

199 Here, we screened a panel of Cas9 nucleases and identified a small Cas9 ortholog from

200 CRISPR-Cas9 nucleases are powerful genome engineering tools, bu

201 Cas9 nucleases complexed with a guide RNA (Cas9-gRNA) fi

202 Unfortunately, currently available small Cas9 nucleases either display low activity or require a

203 ll division in newly created human PT CRISPR/Cas9 OCRL knockout cells, multiple PT cell lines treated

204 f two-cell mouse embryos using either CRISPR-Cas9 or base editors.

205 about the factors responsible for dislodging Cas9 or how they influence genome engineering.

206 demonstrated its utility in multiple CRISPR/CAS9 or siRNA HTS studies.

207 nel of Cas9 nucleases and identified a small Cas9 ortholog from Staphylococcus auricularis (SauriCas9

208 The more compact Type II-C Cas9 orthologs can help to overcome the size limitation

209 Characterization of a subset of Cas9 orthologs using purified components reveals additio

210 tochrome genes using highly-efficient CRISPR-Cas9 procedures.

211 mRNA (100%) compared to those injected with Cas9 protein (94.2%), with little to no unintended off-t

212 bles the application of a nuclease competent Cas9 protein for transcriptional modulation of genes, al

213 ommonly associated with excessive amounts of Cas9 protein, we have developed a series of novel UAS-Ca

214 ic deletions in combination with recombinant Cas9 protein.

215 PAM) and guide RNA (gRNA) requirements of 79 Cas9 proteins, thus identifying at least 7 distinct gRNA

216 nes temperate phages encode up to three anti-Cas9 proteins, with acrIIA1 always present.

217 prophages encode two to three distinct anti-Cas9 proteins, with acrIIA1 always present.

218 Cas9 recognizes its target site by unwinding the DNA dou

219 Using nucleofection-based delivery of Cas9-ribonuclear proteins (RNPs), we achieved near popul

220 cluding mRNA, Cas9 mRNA/single guide RNA and Cas9 ribonucleoprotein complexes, and is envisioned to a

221 directed repair (HDR) template interact with Cas9 ribonucleoproteins (RNPs) to shuttle the template t

222 can mediate efficient controlled delivery of Cas9 RNP in vitro and in vivo.

223 nt intracellular delivery and the release of Cas9 RNP into 293T cells and colorectal cancer (CRC) cel

224 Furthermore, stabilizing Cas9 RNPs into nanoparticles with polyglutamic acid furt

225 To improve Cas9's homology directed repair capacity, here we report

226 capable of inhibiting Staphylococcus aureus Cas9 (SauCas9), an alternative to the most commonly used

227 (2020) conduct a focused CRISPR/Cas9 screen against NRF2 target and other redox regulato

228 ls, a second, subsequent whole-genome CRISPR-Cas9 screen identified the LITAF-like protein CDIP1 as a

229 Here we performed a CRISPR-Cas9 screen in human SV589 cells for genes required for

230 the impact of cellular p53 status on CRISPR-Cas9 screen performance, we carried out parallel CRISPR-

231 rge-scale drug sensitivity, RNAi, and CRISPR-Cas9 screening data from 460 cell lines.

232 scRNA-seq, ATAC-seq and genome-scale CRISPR-Cas9 screening, we identify pathways and genes likely to

233 performance, we carried out parallel CRISPR-Cas9 screens in wild-type and TP53 knockout human retina

234 ammalian cell lines, such as RNAi and CRISPR-Cas9 screens, have made major contributions to the eluci

235 ses its two-domain architecture to act as a "Cas9 sensor," tuning acr expression according to Cas9 le

236 ur new algorithm outperforms existing CRISPR/Cas9 sgRNA design tools.

237 e that 5'-and 3'-nucleotide overhangs negate Cas9/sgRNA catalytic activity in vivo.

238 ough-deficient cells, generated using CRISPR-Cas9, showed increased MTCH2 expression and, consistent

239 rIIA20 strongly inhibits Streptococcus iniae Cas9 (SinCas9) and weakly inhibits Streptococcus pyogene

240 ia their ability to join to specific genomic Cas9/single-guide RNA-generated bait DSBs.

241 he targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely res

242 enome editing protein Streptococcus pyogenes Cas9 (SpyCas9), we used both self-targeting CRISPR scree

243 ) and weakly inhibits Streptococcus pyogenes Cas9 (SpyCas9).

244 iques, including a highly regulatable CRISPR/Cas9 strategy to induce DNA double strand breaks specifi

245 53+/+ cells compared to TP53-/- cells and DD-Cas9, suggesting that differences in break sensing are r

246 Here we use genome-wide CRISPR-Cas9 suppressor screens to identify the oxidative organe

247 ncoding CD137L) in NOD mice using the CRISPR/Cas9 system (designated NOD.Tnfsf9 (-/-)).

248 terspaced short palindromic repeats (CRISPR)-Cas9 system and a transposon-disrupted allele.

249 The CRISPR-Cas9 system has increased the speed and precision of gen

250 activity and outperforms a state-of-the-art Cas9 system in identifying essential genes.

251 The CRISPR/Cas9 system is a powerful method of editing genes by ran

252 ome engineering technologies based on CRISPR/Cas9 system is enabling people to systematically underst

253 We used the CRISPR/Cas9 system to delete CD38 (CD38KO) in ex vivo expanded

254 ocus on reducing mosaicism when using CRISPR/Cas9 system to facilitate direct functional analysis in

255 onstrate the feasibility of using the CRISPR-Cas9 system to model loss of candidate tumor suppressor

256 Gla floxed mouse (Mgp.floxed) by the CRISPR/Cas9 system, that subsequently allowed the generation of

257 ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system.

258 larly interspaced short palindromic repeats)/Cas9 system.

259 ummarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applications in c

260 can be activated or suppressed using CRISPR--Cas9 systems.

261 Truncated Cas9 target sequences (tCTSs) added at the ends of the h

262 e CMV-driven Cre in mouse, and rbm24a-CRISPR/Cas9-targeted mutation or morpholino knockdown in zebraf

263 In Enterococcus faecalis, conjugation of a Cas9-targeted plasmid was enhanced by anti-CRISPRs deriv

264 In the present study, we describe nanopore Cas9-targeted sequencing (nCATS), an enrichment strategy

265 ltifragment ligation strategy and the CRISPR-Cas9 technique, respectively to investigate the signific

266 CRISPR/Cas9 technologies have revolutionized our understanding

267 cell lines constructed with siRNA and CRISPR/Cas9 technologies to vary only in NAT1 N-acetylation act

268 , while knockout of GmPRR3b(H6) using CRISPR/Cas9 technology delayed growth and the floral transition

269 ed with PPRD, which were generated by CRISPR-Cas9 technology displayed low level of expression of mut

270 Our approach thus adapts the CRISPR/Cas9 technology for memory CD8 T cells to undertake gene

271 proaches: the genetic edition through CRISPR/Cas9 technology of genes encoding STING or cGAS in NIH/3

272 le of Eprs globally (Eprs(+/-)) using CRISPR-Cas9 technology or in a Postn-Cre-dependent manner (Eprs

273 CRISPR-Cas9 technology represents a promising platform for achi

274 K/MYD88 signaling in PEL, we employed CRISPR/Cas9 technology to generate stable deletion clones in BC

275 fxn3 (encoding sideroflexin-3), using CRISPR/Cas9 technology.

276 ng species diffusing in living cells: CRISPR-Cas9, TetR, and LacI.

277 To further the utility of Cas9 there have been efforts to achieve temporal control

278 We used CRISPR/Cas9 to create nematodes carrying an in-frame deletion o

279 genomic analysis was performed, using CRISPR-Cas9 to delete MafK-int6 binding region in IRF8 expressi

280 Here, we used CRISPR/Cas9 to delete ZNF274 binding sites at the SNORD116 locu

281 We used CRISPR-Cas9 to engineer human cell lines expressing POLE tumor

282 ok a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose s

283 larly interspaced short palindromic repeats)/Cas9 to introduce indels in exon 3 of FMR1, we generated

284 es targeted cleavage of chromosomal DNA with Cas9 to ligate adapters for nanopore sequencing.

285 ll models and functional assays using CRISPR/Cas9 to study TNNT2 variant pathogenicity and pathophysi

286 cells in which PKA has been deleted (CRISPR-Cas9) to identify PKA-independent responses to vasopress

287 ategy to rapidly evolve other desired CRISPR-Cas9 traits besides enhanced fidelity, to expand the uti

288 ein, we have developed a series of novel UAS-Cas9 transgenes, which allow fine tuning of Cas9 express

289 complex FACT (SPT16 and SSRP1) in governing Cas9 turnover at the DNA target site during genome and e

290 ks (DSBs) that we apply in parallel to eight Cas9 variants across 59 targets.

291 y genome editing experiment, and a number of Cas9 variants have been reported that improve specificit

292 ort that AcrIIA1 binds with high affinity to Cas9 via the catalytic HNH domain.

293 ssion, which was further evidenced by CRISPR/Cas9-VPR-mediated activation of NICI expression.

294 nza-resistant LNCaP-95 cells in which CRISPR-Cas9 was used to knockout AR-FL or AR-V7 alone or in com

295 Using CRISPR-Cas9, we disrupted the expression of ZFP628 in the mouse

296 Using the RNA-guided nuclease Cas9, we induced two DNA double-strand breaks, one each

297 Using CRISPR-Cas9, we introduced frameshift mutations in these risk g

298 in male and female mice, generated by CRISPR/cas9, we show here that the KRK motif in the PlxnA4 cyto

299 es of Cas9, creating a platform for endowing Cas9 with diverse functions.

300 Using a CRISPR/Cas9 Zebrafish her6::Venus reporter combined with mathem