ライフサイエンスコーパス: Ci

1 Ci uses multiple Ser/Thr (S/T)-rich motifs that bind HIB

2 Ci variants that cannot be processed supported a normal

3 Ci(155) functions as a transcriptional activator of a nu

4 Ci-Dll-B is located in a convergently transcribed bigene

5 Ci-MRF is the sole myogenic regulatory factor (MRF) of t

6 Ci-Slc26aalpha acts as an anion transporter, mediating t

7 Ci-Slc26aalpha is indispensable for lumen formation and

8 Ci-VSD can be reconstituted into liposomes of various co

9 ecific activity of 40-111 GBq/mumol (1.1-3.0 Ci/mumol).

10 (+/-SD) was 7 +/- 2 GBq/mumol (0.19 +/- 0.05 Ci/mumol), and radiochemical purity was greater than 99%

11 Starting with 29.6-37 GBq (0.8-1 Ci) of (18)F-fluoride, more than 7.4 GBq (>200 mCi) of (

12 activities of at least 10 microCi/microg (1 Ci = 37 GBq) were routinely achieved, and no additional

13 ific activity (2.1 GBq/mmol, 58 mCi/mmol) (1 Ci = 37 GBq) and no detectable dilution of label from en

14 g nearly a gram of antibody with 481 GBq (13 Ci) of (131)I during a single 30-min reaction run.

15 onal distribution is found: between delta(13)Ci = -11 and -53 per thousand.

16 al abundance distribution of (13)C (delta(13)Ci) within the molecule with better than 1 per thousand

17 ific activity of 8.0 x 10(7) MBq/mmol (2,166 Ci/mmol) and a radiochemical purity greater than 98%.

18 /- 3% and a specific activity of 2.2 +/- 0.2 Ci/micromol.

19 2%, and specific activity was 44.4 GBq (1.2 Ci)/mumol.

20 P) and specific activity (SA) of 2.6 +/- 1.2 Ci/mumol (n = 13) at end of synthesis (EOS).

21 l [(3)H]2-AG with a specific activity of 200 Ci/mmol for enzyme assays, metabolic studies, and tissue

22 urity at high specific activity (>111 GBq [3 Ci]/mumol).

23 at the notochord expression of Ciona Tbx2/3 (Ci-Tbx2/3) requires Ci-Bra, and identified a Ci-Tbx2/3 n

24 fic activity of 18,500-48,100 GBq (500-1,300 Ci)/mmol.

25 )H]PSB-13253, with a specific activity of 36 Ci (1.33 TBq)/mmol.

26 c activities of 666 +/- 51.8 GBq (18 +/- 1.4 Ci)/mumol at the end of synthesis and was validated for

27 activity (666 +/- 51.8 GBq/mumol; 18 +/- 1.4 Ci/mumol).

28 on) and a specific activity of 39.0 +/- 12.4 Ci mumol(-1) within 77 +/- 4 min.

29 n beam current, producing up to 348 GBq (9.4 Ci) of (99m)Tc.

30 Sch225336 has high specific activity (>1,400 Ci/mmol) and affinity for hCB2 (65 pm).

31 ]propylbicycloorthobenzoate ([(3)H]EBOB) (46 Ci/mmol), illustrating the use of AMS for characterizing

32 ru-2,6-P(2) having a specific activity of 50 Ci/mmol was obtained in yields averaging 35%.

33 suggest that a center receiving 1.85 TBq (50 Ci) of (99)Mo once every 4 d can provide 1.48-3.33 TBq (

34 injectable [(18)F]7 from up to 285 GBq (7.7 Ci) of [(18)F]fluoride in 50 min (uncorrected radiochemi

35 pound was 853 +/- 29.6 GBq/mumol (23 +/- 0.8 Ci/mumol).

36 activity (76 +/- 30 TBq/mmol, 2,050 +/- 804 Ci/mmol), n = 26.

37 e every 4 d can provide 1.48-3.33 TBq (40-90 Ci) of (99m)Tc daily.

38 nprecedented high specific activities (50-99 Ci/mmol) in radiolabeling, meeting the threshold require

39 This complex controls the level of TRA-1A, a Ci/Gli homolog and master regulator of sex determination

40 in states of the photosynthetic apparatus: a Ci-limited state and a light-limited state.

41 Ci-Tbx2/3) requires Ci-Bra, and identified a Ci-Tbx2/3 notochord CRM that necessitates multiple Ci-Br

42 of aPKC is up-regulated by Hh signaling in a Ci-dependent manner.

43 We also provide evidence that PP2A is a Ci phosphatase.

44 We used a global dataset of A-Ci curves (564 species from 46 field sites, covering a r

45 Estimating this parameter using A-Ci curves (net photosynthesis, A, vs intercellular CO2 c

46 rate vs internal CO2 concentration curves (A/Ci ), and relationships with foliar nitrogen (N) and P c

47 released by CAH3 dehydration of accumulated Ci.

48 nvergent total synthesis of (-)-nahuoic acid Ci(Bii) (3), a novel cis-decalin polyketide, has been ac

49 family gene, and diverse mechanisms activate Ci-Mrf Here, we show that myogenesis in the atrial sipho

50 itro by the muscle-specific T-box activators Ci-Tbx6b and Ci-Tbx6c.

51 in HLA3 is proposed to be involved in active Ci uptake across the plasma membrane.

52 These phosphorylation events alter Ci binding to the pathway inhibitor Suppressor of fused

53 upon the transcription factors Ci-ets1/2 and Ci-mesp to generate cardiac progenitors.

54 d, resulted in increased Ci accumulation and Ci-dependent photosynthetic O2 evolution specifically in

55 decreases in photosynthetic Ci affinity and Ci uptake, the combination of nearly complete knockdown

56 e orthologous transcription factors Gli2 and Ci, respectively.

57 malian kinesin Kif7 can also direct Gli3 and Ci processing in fly, underscoring a fundamental conserv

58 We provide evidence that both HIB and Ci form dimers/oligomers and engage in multivalent inter

59 or voltage-sensing domain proteins (Hv1 and Ci-VSP) into eukaryotic voltage-activated potassium chan

60 lose-1,5-bisphosphate, phosphoglycerate, and Ci pools when grown under comparable conditions.

61 55 contributes to both Ci-155 processing and Ci-155 silencing in the absence of Hh.

62 influence Hh signaling by regulating Smo and Ci, respectively.

63 late Hh signaling by phosphorylating Smo and Ci; however, the phosphatase(s) involved remain obscured

64 nterfering with the formation of Ci-Sufu and Ci-Cos2-kinase complexes that normally inhibit Ci activi

65 uscle-specific T-box activators Ci-Tbx6b and Ci-Tbx6c.

66 H(+) 'shuttle' conductance (GSH) in VGCs and Ci VSP, and we now report that R1H is sufficient to reco

67 to the intracellular components to attenuate Ci cleavage.

68 e have obtained evidence that Wdb attenuates Ci processing probably by dephosphorylating Ci.

69 O(3)(-) is the predominant form of available Ci.

70 Here we show that Sufu can bind Ci/Gli through a C-terminal Sufu-interacting site (SIC)

71 he CORD domain of Ci-155 contributes to both Ci-155 processing and Ci-155 silencing in the absence of

72 onal binding sites for both Ciona Brachyury (Ci-Bra) and FoxA (Ci-FoxA-a).

73 We found that Ciona Brachyury (Ci-Bra) controls most of its targets directly, through n

74 ecific transcription factor Ciona Brachyury (Ci-Bra).

75 the known enhancer that regulates Brachyury (Ci-Bra), a key determinant of notochord specification.

76 34 potential noncoding RNAs was affected by Ci supply, although most of these molecules were not reg

77 gh the same T-box sites that are utilized by Ci-Bra in the notochord, which are also bound in vitro b

78 wth, photosynthetic Ci affinity, and [(14)C]-Ci uptake in very low CO(2) conditions following RNA int

79 anobacterium to changes in inorganic carbon (Ci) availability.

80 under different light and inorganic carbon (Ci) conditions as well as under genetic perturbations.

81 respiration in response to inorganic carbon (Ci) limitation.

82 sition and assimilation of inorganic carbon (Ci) represents the largest flux of inorganic matter in p

83 Active inorganic carbon (Ci) uptake, Rubisco sequestration and interconversion be

84 hydrases (CAs), and active inorganic carbon (Ci) uptake.

85 tii acclimates to limiting inorganic carbon (Ci), either low-CO2 (L-CO2; air level; approximately 0.0

86 cyanobacteria with similar inorganic carbon (Ci; as CO2 and HCO3-) transporter systems.

87 ncluding identification of inorganic carbon (Ci; CO(2) and HCO(3)(-)) transporters; however, specific

88 ctly to the unliganded asymmetric "carrier" (Ci) distribution.

89 transport and a role for LCIB in chloroplast Ci accumulation.

90 cited scientists, using the total citations Ci of each scientist as his/her reputation measure.

91 observed that Li is related with the clicks (Ci) to news stories and the age (Ti) of stories.

92 tomatal conductance (gs), intercellular CO2 (Ci), chlorophyll (Chl) content in WT plants as compared

93 second, through increased leaf internal CO2 (Ci ) and decreased stomatal limitations (Slim ).

94 s-derived reduction in intercellular [CO2 ] (Ci ) remains controversial and genetic analyses are need

95 esis, A, vs intercellular CO2 concentration, Ci ) is laborious, which limits availability of Vcmax da

96 urbation of phosphoinositide concentrations, Ci-VSPTEN will be useful for probing the role and specif

97 orphology, when supplemented by constitutive Ci repressor, showing that Hh can signal normally in the

98 oxysome types showed a response to cytosolic Ci, which is of higher affinity than predicted by curren

99 ), higher relative Slim (>30%) and decreased Ci under the ambient CO2 concentration (aCO2 ), with lea

100 formation energies of the C related defects Ci(SiI), CiOi, CiCs, and CiOi(SiI) with respect to the F

101 Ci processing probably by dephosphorylating Ci.

102 ration and interconversion between different Ci species catalyzed by carbonic anhydrases (CAs) are ke

103 ar concentration of Ci as measured by direct Ci uptake.

104 amily protein Costal 2 (Cos2), which directs Ci processing in Drosophila.

105 eolytic processing of full-length Drosophila Ci or mammalian Gli proteins to nuclear transcriptional

106 a putative vertebrate ortholog of Drosophila Ci, cause nephronophthisis type 7 in humans and mice.

107 ibed bigene cluster with a tandem duplicate, Ci-Dll-A.

108 Thus, cis-regulation of early Ci-Dll-B expression is activated by a required submodule

109 at Cos2 must bind to Fu to support efficient Ci-155 processing.

110 st protocol for the expression of eukaryotic Ci-VSD in Escherichia coli at milligram levels.

111 onse of photosynthesis to light and external Ci and their modulation of internal ribulose-1,5-bisphos

112 ned (Smo) and Zn-finger transcription factor Ci/Gli are crucial components in Hedgehog (Hh) signal tr

113 hat of vertebrates but only one GATA factor, Ci-GATAa, is expressed in the heart progenitor cells and

114 is dependent upon the transcription factors Ci-ets1/2 and Ci-mesp to generate cardiac progenitors.

115 binds to three regions of Gli1, just as for Ci, and that Cos2 functions to silence mammalian Gli1 in

116 hat this transport activity is essential for Ci-Slc26aalpha's in vivo function.

117 ve in the main transcriptional repressor for Ci acquisition genes, the NAD(P)H dehydrogenase transcri

118 nterruptus (Ci)/Gli into its activator form (Ci(A)/Gli(A)), but the underlying mechanism remains poor

119 for both Ciona Brachyury (Ci-Bra) and FoxA (Ci-FoxA-a).

120 The calculated mass fraction (Ci,aer/COA) of the individual SOA compounds showed eithe

121 ion causes PKA to switch its substrates from Ci to Smo within the Hh signalling complex (HSC).

122 The voltage dependence of the VSD from Ci-VSP (Ci-VSD) is dramatically right shifted, so that a

123 Like mammalian Lhx3 genes, Ci-Lhx3 encodes two isoforms with distinct N-terminal pe

124 d and essential for proper regulation of Gli/Ci proteins in the Hh pathway.

125 Here, we report that Dzip1 regulates Gli/Ci turnover by preventing degradation of speckle-type PO

126 l transduction pathways terminating with Gli/Ci transcription factors.

127 ownstream transcription factor NvGli (a Gli3/Ci ortholog) indicate that these genes may have conserve

128 )) resulted in dramatic decreases in growth, Ci uptake, and photosynthetic Ci affinity, especially at

129 tes are specifically bound in vitro by a GST-Ci-Bra fusion protein, and mutations that abolish bindin

130 In the presence of Hh, Ci-155 processing is blocked and Cos2 now promotes activ

131 In the absence of Hh, Ci/Gli processing is initiated by direct Pka phosphoryla

132 This newly identified Ci-Bra shadow enhancer contains binding sites with very

133 se to Hh, which collaborates with changes in Ci-specific activity to elicit a morphogenetic response.

134 Loss of emc leads to an increase in Ci(155) levels, nuclear migration, apical cell constrict

135 e citation rate for each tenfold increase in Ci.

136 ich confirms that HLA3 is indeed involved in Ci uptake, and suggests it is mainly associated with HCO

137 esting a unique voltage sensing mechanism in Ci-VSP.

138 cs and voltage dependence of VSD movement in Ci-VSP can be tuned over 2 orders of magnitude and shift

139 Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics

140 indicating the importance of this residue in Ci-VSP activation.

141 lpha-to-310 helical conversions of the S4 in Ci-VSP associated with voltage activation.

142 HLA3 is not expressed, resulted in increased Ci accumulation and Ci-dependent photosynthetic O2 evolu

143 f Slim by eCO2 was facilitated by increasing Ci , thus yielding a larger photosynthetic enhancement d

144 mily transcription factors and by increasing Ci/Gli-specific activity.

145 e involvement of additional NdhR-independent Ci-regulatory mechanisms.

146 -Cos2-kinase complexes that normally inhibit Ci activity and promote its processing.

147 tes Hh signaling activity through inhibiting Ci ubiquitination and degradation mediated by both Slimb

148 lasm through binding to SIN while inhibiting Ci/Gli activity in the nucleus depending on SIC.

149 s mechanistic insight into how Sufu inhibits Ci/Gli activity in the nucleus.

150 e molecular mechanism by which Sufu inhibits Ci/Gli activity remains poorly understood.

151 nic anhydrase CAH6 of the very high internal Ci caused by the defect in CAH3 provides Rubisco suffici

152 er transcription factor Cubitus interruptus (Ci(155)), the main effector of Hh signaling.

153 he transcription factor Cubitus interruptus (Ci) and G protein coupled receptor (GPCR) family protein

154 ion factors, Drosophila Cubitus interruptus (Ci) and mammalian Gli proteins.

155 activated Fu regulates Cubitus interruptus (Ci) by both promoting its transcriptional activator acti

156 The Gli/Cubitus interruptus (Ci) family of transcription factors acts at the downstre

157 ulating the activity of Cubitus interruptus (Ci) through phosphorylation of the Zn finger DNA-binding

158 g the repressor form of Cubitus interruptus (Ci), a Hh pathway antagonist, also results in expansion

159 matin binding sites for Cubitus interruptus (Ci), the transcription factor that mediates outputs of H

160 tional mediator, called Cubitus interruptus (Ci).

161 tability of full-length Cubitus interruptus (Ci).

162 nt transcription factor cubitus interruptus (Ci).

163 he transcription factor Cubitus interruptus (Ci).

164 he transcription factor Cubitus interruptus (Ci).

165 ay transcription factor cubitus interruptus (Ci)/Gli by Cul3-Hedghog-induced MATH and BTB domain-cont

166 ls of homology with the Cubitus interruptus (Ci)/Gli family of proteins.

167 nt transcription factor Cubitus interruptus (Ci)/Gli into its activator form (Ci(A)/Gli(A)), but the

168 g pathway by inhibiting Cubitus interruptus (Ci)/Glioma-associated oncogene homolog (Gli) transcripti

169 the prototypic VSP from Ciona intestinalis, Ci-VSP, we generated chimeric proteins that are voltage-

170 te of a significantly elevated intracellular Ci concentration.

171 We studied intracellular Ci limitation in the slow-growing CO2/HCO3 (-)-uptake mu

172 Synechocystis sp. PCC 6803 to intracellular Ci limitation and may become a valuable reference for im

173 ance of photorespiration under intracellular Ci limitation.

174 /3 targets, some of which overlap with known Ci-Bra-downstream notochord genes.

175 CO2 levels in leaves (Ci) are determined by respiration, photosynthesis, stoma

176 2 phosphorylation and activating full-length Ci by antagonizing Su(fu) and by other mechanisms.

177 repressors and by activating the full-length Ci or Gli proteins.

178 omplex regulates the cleavage of full-length Ci to a truncated repressor protein, Ci75, in a process

179 a full Hh response, stabilizing full-length Ci via Cos2 phosphorylation and activating full-length C

180 necessary for full activation of full-length Ci-155 and Gli transcription factors in response to Hh p

181 l and thus signaling activity of full-length Ci.

182 edgehog (Hh) signaling activates full-length Ci/Gli family transcription factors and prevents Ci/Gli

183 ockdown of LCIA mRNA (which encodes limiting-Ci-inducible plastid envelope protein reported to transp

184 th mutations in LCIB (which encodes limiting-Ci-inducible plastid-localized protein required for norm

185 ca1ca4 plants suggest that more than a low [Ci ]-dependent pathway may function in red light-induced

186 reacclimates cyanobacterial cells to lowered Ci supply under HC conditions.

187 ced relaxation EPR spectroscopy measurement, Ci-VSD reconstitutes essentially randomly in proteolipos

188 , S572 and S931, which is thought to mediate Ci-155 activation, is not required for normal activation

189 erminal regions of Ci, both of which mediate Ci degradation.

190 Each gel was radiolabeled with 500 micro Ci of (99m)Technetium-diethylene triaminepentaacetic aci

191 simple in vivo assay based on misexpressing Ci-MRF in the notochord of Ciona embryos.

192 urrent models, being saturated by 5 to 15 mm Ci.

193 d that a specific Ephrin signaling molecule, Ci-ephrin-Ad, is required to establish asymmetric MAPK s

194 Mice treated with anti-CD20 PRIT and 600 mu Ci [(2)(1)(3)Bi]DOTA-biotin exhibited marked tumor growt

195 2/3 notochord CRM that necessitates multiple Ci-Bra binding sites for its activity.

196 affold yielded similar results as the native Ci-VSP S4.

197 lastid-localized protein required for normal Ci uptake or accumulation in low-CO(2) conditions) and/o

198 he Ala-Thr dipeptide is necessary for normal Ci-MRF function, and that while eliminating the C/H doma

199 Here, we show that normal Ci-155 activation by Hh requires Cos2 binding to Fu, sup

200 gh microarray screens, we uncovered numerous Ci-Tbx2/3 targets, some of which overlap with known Ci-B

201 on, is not required for normal activation of Ci-155 by Hh or by activated Fu.

202 blocked and Cos2 now promotes activation of Ci-155, which requires Fu kinase activity.

203 n by regulating the levels and activities of Ci/Gli family transcription factors.

204 tion, regulating the amounts and activity of Ci that ultimately interpret the level of Hh to which ce

205 quisite for generating sufficient amounts of Ci-VSD protein for high-resolution structural studies.

206 crease in the intracellular concentration of Ci as measured by direct Ci uptake.

207 We demonstrate that experimental control of Ci-VSPTEN can be obtained either by electrophysiological

208 ocopy is associated with the deregulation of Ci control, an overreduced cellular state, and limited p

209 ly acting through binding the CORD domain of Ci, or PKA, revealing separate inhibitory roles of these

210 ence that Cos2 binding to the CORD domain of Ci-155 contributes to both Ci-155 processing and Ci-155

211 ttern mimicking the endogenous expression of Ci-Dll-B at gastrula stages.

212 tor induction through targeted expression of Ci-Integrin beta2.

213 sphorylation of Ci leads to the formation of Ci repressor form.

214 lations by interfering with the formation of Ci-Sufu and Ci-Cos2-kinase complexes that normally inhib

215 Expression of mutant forms of Ci-Tbx2/3 in the developing notochord revealed a role fo

216 es both the activator and repressor forms of Ci.

217 mids aimed to interfere with the function of Ci-leprecan and categorized the resulting phenotypes, wh

218 d SIN are required for optimal inhibition of Ci/Gli by Sufu.

219 Our data suggest multiple layers of Ci regulation, including inversely regulated modules of

220 homolog of Spop, and increased the level of Ci.

221 the HSC, and in which the relative levels of Ci and Smo within the HSC determine differential activat

222 alpha and CK2beta increases the half-life of Ci.

223 Conversely, misexpression of Ci-ephrin-Ad in the endoderm induces ectopic activation

224 te that Hh stimulates the phosphorylation of Ci by the Ser/Thr kinase Fused (Fu) and that Fu-mediated

225 on, whereas PKA-dependent phosphorylation of Ci leads to the formation of Ci repressor form.

226 (Fu) and that Fu-mediated phosphorylation of Ci promotes its activation.

227 Fu-family kinase-mediated phosphorylation of Ci/Gli serves as a conserved mechanism that activates th

228 When Hh is absent, phosphorylation of Ci/Gli triggers binding to SCF ubiquitin ligase complexe

229 by inhibiting the proteolytic processing of Ci/Gli family transcription factors and by increasing Ci

230 ubiquitin/proteasome-mediated proteolysis of Ci/Gli transcription factors is central to Hh signaling,

231 ding of Sufu to SIC and the middle region of Ci can impede recruitment of the transcriptional coactiv

232 binding primarily through a nearby region of Ci, which might contact an SCF component other than Slim

233 its binding site in the C-terminal region of Ci.

234 fu-binding motif in the C-terminal region of Ci/Gli and provides mechanistic insight into how Sufu in

235 HIB binds the N- and C-terminal regions of Ci, both of which mediate Ci degradation.

236 Studies of Ci variants with altered CK1 and GSK3 sites suggest that

237 B cluster reveals a 378bp region upstream of Ci-Dll-B, termed B1, which is highly conserved with the

238 tal limitation of CO2 uptake, by control of [Ci ], was eliminated.

239 I individually has no demonstrable effect on Ci-MRF, simultaneous loss of both motifs significantly r

240 e evidence for an analogous action of PKA on Ci.

241 ple successively phosphorylated CK1 sites on Ci create an atypical extended binding site for the SCF

242 ntee optimal growth under excessive light or Ci limitation.

243 ses the level of Smo, which then outcompetes Ci for association with PKA and causes a switch in PKA s

244 The voltage-sensitive phosphatase Ci-VSP consists of an intracellular phosphatase domain (

245 The voltage-dependent phosphatase (Ci-VSP), which does not have a conducting pore, shows th

246 na intestinalis voltage-sensing phosphatase (Ci-VSP)-induced PIP(2) depletion, whereas activation of

247 intestinalis voltage-sensitive phosphatase (Ci-VSP) does not exhibit extended and long-lived 310 con

248 intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzyme

249 We find that Fu directly phosphorylates Ci on Ser218 and Ser1230, which primes its further phosp

250 ses in growth, Ci uptake, and photosynthetic Ci affinity, especially at pH 9, at which HCO(3)(-) is t

251 g the effect of pH on growth, photosynthetic Ci affinity, and [(14)C]-Ci uptake in very low CO(2) con

252 igh-pH-dependent decreases in photosynthetic Ci affinity and Ci uptake, the combination of nearly com

253 find that Wdb counteracts kinases to prevent Ci phosphorylation.

254 li family transcription factors and prevents Ci/Gli proteolytic processing to repressor forms.

255 We showed that CK2 prevents Ci ubiquitination and degradation by the proteasome.

256 CK2beta RNAi promotes Ci degradation whereas coexpressing CK2alpha and CK2beta

257 to their well-established roles in promoting Ci processing.

258 Reducing Ci-ephrin-Ad activity via morpholino injection results i

259 r divergence in the mechanisms that regulate Ci/Gli protein activities, including the role of the kin

260 are attenuated in their ability to regulate Ci activity and exhibit phenotypes consistent with atten

261 H) protein Extramacrochaetae (Emc) regulates Ci(155) levels.

262 mine the mechanism by which Su(fu) regulates Ci import by investigating the importance of the Ci nucl

263 osphatase Puc, the transcriptional regulator Ci, and the chromatin component MacroH2A.

264 ing site to an "exposed" location can render Ci resistant to Sufu-mediated inhibition in the nucleus.

265 ression of Ciona Tbx2/3 (Ci-Tbx2/3) requires Ci-Bra, and identified a Ci-Tbx2/3 notochord CRM that ne

266 Residual Ci-155 processing in the absence of Cos2-Fu interaction

267 The processing-resistant Ci variants were also significantly activated in the abs

268 smembrane Smoothened (Smo) proteins reverses Ci/Gli inhibition by Suppressor of Fused (SuFu) and kine

269 ame this obstacle by developing large-scale (Ci-scale) production and purification methods for (134)C

270 We show that Sufu can sequester Ci/Gli in the cytoplasm through binding to SIN while inh

271 indicating a deficiency in a L-CO2-specific Ci uptake and accumulation system.

272 engineered voltage-sensitive enzymes, termed Ci-VSPTEN, is the novel ability to switch enzymatic acti

273 The Ci(SiI)(2+) state dominates in almost the whole Fermi en

274 The Ci-Dll-B gene is an early regulator of ectodermal develo

275 The Ci/Gli family of transcription factors mediates Hedgehog

276 Among the Ci-Tbx2/3 notochord targets are evolutionarily conserved

277 We examined the Ci-dependent transcriptional and metabolic regulation in

278 icrocystis displays genetic variation in the Ci uptake systems BicA and SbtA, where BicA has a low af

279 prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed f

280 x-ray structures, its transplantation in the Ci-VSP VSD scaffold yielded similar results as the nativ

281 nt that generates the repressor forms of the Ci and Gli transcription factors that keep target genes

282 mport by investigating the importance of the Ci nuclear localization signal (NLS) and the effect of a

283 By screening approximately 14 kb of the Ci-leprecan locus for cis-regulatory activity, we have i

284 TRA-1, a member of the Ci/Gli family of transcriptional repressors, plays an es

285 trained model of the hHv1 dimer based on the Ci-VSD structure at resting state.

286 rbol-12-myristate-13-acetate potentiated the Ci-VSP-induced decline in Kv7.5 current amplitude.

287 Thus, Ci-VSPTEN provides a novel approach for studying the com

288 serpentine protein Smoothened (Smo) leads to Ci activation, whereas PKA-dependent phosphorylation of

289 v7.5 based on differential responsiveness to Ci-VSP activation and different rates of current rundown

290 ing of a ubiquitin-specific protease Usp7 to Ci, which positively regulates Hh signaling activity thr

291 om the preacclimation of the transcriptional Ci regulator mutant ndhR (for ndhF3 operon transcription

292 The 155-bp sequence contains two Ci-Bra binding sites with identical core sequences but o

293 We used Ci variants expressed at physiological levels to investi

294 e voltage dependence of the VSD from Ci-VSP (Ci-VSD) is dramatically right shifted, so that at 0 mV i

295 lake, bicA + sbtA strains were dominant when Ci concentrations were depleted during a dense cyanobact

296 rains with only the high-flux bicA gene when Ci concentrations increased later in the season.

297 We also show that when Ci proteolysis is compromised, its specific activity is

298 ndings reveal a conserved mechanism by which Ci/Gli is stabilized by a deubiquitination enzyme and id

299 iofuel production in cyanobacteria, in which Ci is channeled off from central metabolism and may thus

300 terns' of transcription factors partner with Ci to make Hh-dependent gene expression position specifi