ライフサイエンスコーパス: Cy3

1 nctional, N-hydroxysuccinimidyl fluorophore (Cy3).

2 to allow (111)In labeling or the fluorophore Cy3.

3 nced by the region of the protein contacting Cy3.

4 tions of the NTA derivative, (Ni(2+)-NTA)(2)-Cy3.

5 cDNA of the healthy co-twin was labeled with cy3.

6 pping a 15-basepair DNA segment labeled with Cy3.

7 asuring diffusional transfer of biocytin and Cy3.

8 erative visualization of tissue stained with Cy3.

9 proximately 110 positive genes detected with Cy3.

10 were detected by RLS compared to labeling by Cy3.

11 orochrome FITC and the acceptor fluorochrome Cy3.

12 odies conjugated to the orange emitting dye, Cy3.

13 the signal of the apoptosis marker Annexin V-Cy3.

14 ed in the resonance energy transfer to sulfo-Cy3.

15 d bis-styryl (MeOPh)2BODIPY dyes relative to Cy3.

16 an extrinsic fluorophore [fluorescein (F) or Cy3].

17 r, an intramolecular heterodimer between the Cy3.5 fluorophore and the BHQ1 quencher.

18 and quencher, and with no stem structure: 5'Cy3.5-beta-actin-3'BHQ1 and 5'FAM-beta-actin-3'BHQ1.

19 t is the dominant quenching mechanism in the Cy3.5-BHQ1 probe.

20 Cy3-labelled MBP bound to beta-cyclodextrin-Cy3.5.

21 The 30SIC(Cy3):50S(Cy5) FRET signal also provides a sensitive prob

22 anthocyanins in commercialised samples were Cy3,5dG (19.30 +/- 3.47 mg/L), followed by Dp3,5dG (17.8

23 di (Dp3,5dG) and cyaniding 3,5-diglucosides (Cy3,5dG).

24 streptavidin conjugated to indocarbocyanine (Cy3), a fluorescent marker.

25 ort that small molecules, such as cyanine 3 (Cy3), a synthetic fluorescent molecule, and 4-hydroxy-3-

26 nsity that results when the protein contacts Cy3, a property sometimes referred to as protein-induced

27 ransfer (FRET) from the fluorescein donor to Cy3 acceptor by splitting these two dyes into separate f

28 A hybridization event that brought the Cy3 acceptor dye in close proximity to the surface of im

29 A coumarin donor and a Cy3 acceptor were positioned at opposite ends of a 23-bp

30 ed with the yellow emissive indocarbocyanine Cy3 acceptor.

31 en-emitting QDs (gQDs) were FRET-paired with Cy3 acceptor.

32 Incorporation of Cy3- actin into lamellipodial protrusions is concomitant

33 -actin assembly after activation of Rac, but Cy3-actin is not incorporated into stress fibers formed

34 mbly, fluorescently labeled monomeric actin, Cy3-actin, was introduced into serum-starved Swiss 3T3 f

35 idin (SAF) or SAF conjugated to biotinylated Cy3 adenoviral-vector (BCAV).

36 Other cyanine dyes (e.g., Cy3, Alexa 555) were not significantly affected until hi

37 ins expressed in living mammalian cells with Cy3, Alexa Fluor 568 and biotin.

38 Labeling with (Ni(2+)-NTA)(2)-Cy3 allows characterization of hydrodynamic properties b

39 form the pentamethine (Cy5) and trimethine (Cy3) analogs.

40 ed-emitting QDs (rQDs) served as donors with Cy3 and Alexa Fluor 647 (A647) acceptors.

41 We observed increased stability of Cy3 and Alexa488 in the presence of the antioxidants asc

42 With a covalently-linked dye pair of Cy3 and Alexa647 to label subcellular structures in fixe

43 ("targets") were hybridized with colabeled (Cy3 and biotin) human lung cDNA probes at concentrations

44 etween DNA and lipid covalently labeled with Cy3 and BODIPY, respectively, was used to monitor the re

45 transfer efficiencies between the juxtaposed Cy3 and Cy5 5'-end labeled viral DNA ends in the synapti

46 n and emission wavelengths of the two labels Cy3 and Cy5 allow for fluorescence resonance energy tran

47 The indodicarbocyanine fluorophores Cy3 and Cy5 are extensively used as donor-acceptor pairs

48 ructure of the indocarbocyanine fluorophores Cy3 and Cy5 attached to DNA via three-carbon atom tether

49 given the mean signal intensity in both the Cy3 and Cy5 channels.

50 labeled, but are spectrally compatible with Cy3 and Cy5 dye-labeled targets when using confocal lase

51 the labeling and hybridization efficiency of Cy3 and Cy5 dyes.

52 Spontaneous membrane partitioning of Cy3 and Cy5 fluorophores captured in these simulations p

53 Global analysis of the Cy3 and Cy5 FRET time-courses, using an n-step sequentia

54 NA derived from the same source was used for Cy3 and Cy5 labeling and hybridized to the same array, s

55 rays to perform, and how to optimally assign Cy3 and Cy5 labels to the specimens.

56 y crystallography to analyze the location of Cy3 and Cy5 on dsRNA, using complexes of an RNA stem-loo

57 A total of 401 genes with Cy3 and Cy5 spot intensities of >/=500 were selected for

58 , anisotropy measurements indicate that both Cy3 and Cy5 stack on the ends of the RNA.

59 ged with fluorescein and are compatible with Cy3 and Cy5 target labeling dyes when using confocal las

60 conjugated with the cyanine dye fluorophores Cy3 and Cy5 to quantify the melting/rebinding reaction b

61 Probes labeled with Cy3 and Cy5 were individually introduced to opposite sid

62 However, the manner of interaction of both Cy3 and Cy5 with the terminus of the dsRNA is significan

63 The data show that Cy-ncAAs (based on Cy3 and Cy5) are tolerated by the eukaryotic ribosome in

64 of nanofibres labelled with different dyes (Cy3 and Cy5) were mixed, and the distribution of dyes in

65 tructed from three chemical components (DNA, Cy3 and Cy5) with tunable spectroscopic properties due t

66 Organic fluorophores, such as Cy3 and Cy5, have been widely used as chemical labels to

67 at the efficiency of energy transfer between Cy3 and Cy5, that are attached to nucleic acids in this

68 from differences in the detection limits of Cy3 and Cy5-labeled cDNA, a potential concern for array

69 ecules are labeled with fluorophores such as Cy3 and Cy5.

70 r two dyes commonly used in DNA microarrays, Cy3 and Cy5.

71 rately conjugated with one of the FRET pair, Cy3 and Cy5.

72 of the shorter product strand containing the Cy3 and increased fluorescence.

73 istinct (approximately 250 nm) fluorophores, Cy3 and IRD800, were excited simultaneously using two di

74 ble-stranded DNA substrate dual labeled with Cy3 and IRD800.

75 hiocyanate (FITC) or to the carbocyanine dye Cy3 and used to label cytokine-responsive human hematopo

76 bp in length) that were labeled with donor (Cy3) and acceptor fluorophores (Cy5).

77 ith cDNAs from control (pH 7.4; labeled with Cy3) and acidic (labeled with Cy5) conditions.

78 nor for energy transfer with both Cyanine 3 (Cy3) and Alexa Fluor 647 (A647) fluorescent dyes associa

79 ary donor array (Py), secondary donor array (Cy3) and an acceptor (AF) with defined interchromophore

80 eled on its 3'-end with a fluorescent donor (Cy3) and on its 5'-end with a fluorescent acceptor (Cy5)

81 g ovarian cancer showed that the model drug (Cy3) and polymer bound to Cy5 were colocalized at an ear

82 metric analysis of the emission from the QD, Cy3, and A647 permits discrimination between thrombin an

83 labeled with a fluorescence donor molecule, Cy3, and fluorescence acceptor molecule, Cy5, and by var

84 obleaching (FRAP) experiments confirmed that Cy3- and BODIPY-FL-labeled ferritin and PZn(2) exhibited

85 ction was demonstrated in this work by using Cy3- and Cy5-doped nanoparticles in sandwich hybridizati

86 Cy3- and Cy5-labeled (and reverse dye-labeled) cDNA prob

87 Diffusion-distance estimates for Cy3- and Cy5-labeled cDNAs were 3.8 mm and 2.6 mm, respe

88 Pairs of cy3- and cy5-labeled extracts from 5-week old Alport and

89 ined the fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distanc

90 ein L11 and A- or P-site Cy5-labeled tRNA or Cy3- and Cy5-labeled tRNAs.

91 tudies indicate that this occurs because the Cy3 anti-GFAP selectively binds the intermediate filamen

92 vivo studies, retinal flatmounts exposed to Cy3 anti-GFAP showed minimal surface fluorescence, but e

93 Cy3 anti-GFAP was injected into the vitreous cavity of e

94 rcine retinal flatmounts were incubated with Cy3 anti-GFAP.

95 intravenous injection of Lac-GLN containing Cy3-anti-miR-155 led to preferential accumulation of the

96 ein (emission maximum approximately 540 nm), Cy3 (approximately 570 nm), Cy5 (approximately 670 nm),

97 Fluorescein and Cy3 are an important donor-acceptor pair of fluorophores

98 en-emitting QDs (gQDs) served as donors with Cy3 as an acceptor.

99 R6G) and 3,3'-Diethyloxacarbocyanine iodide (CY3) as exemplary gain media.

100 t labeled with fluorescein at the 5' end and Cy3 at the 3' end show that modifications required for l

101 Cy3-ATP labeling was substantially reduced-but did not d

102 as performed with fluorescence microscopy of CY3-ATP.

103 nd 2 were labeled particularly strongly with Cy3-ATP.

104 y a change in the fluorescence anisotropy of Cy3 attached to one of the helical ends.

105 nformational dynamics of the fluorescent dye Cy3 attached to the 5' end of double-stranded DNA using

106 etramethylindocarbocyanine-5,5'-disulfonate (Cy3) attached via either a short or long linker at the C

107 oyed in fluorescence microscopy and imaging (Cy3, ATTO-740, and coumarin 343).

108 rent donor-acceptor dye pairs (TMR-ATTO647N, Cy3-ATTO647N, TMR-Cy5, and Cy3-Cy5), we find that this p

109 factors of 10-fold for Cy5 and 2.5-fold for Cy3, because of the smaller amount of immobilized fluoro

110 d the proximity for FRET, with emission from Cy3 being the analytical signal.

111 Using a Cy3/BHQ pair, we can accurately follow conformational ch

112 (Ni(2+)-NTA)(2)-Cy3 binding is reversible in 10mM ethylenediaminetetraac

113 Although shorter N-terminal fragments (Cy3-C0C1 and Cy3-C0C1f) bound to the thin filaments and

114 h shorter N-terminal fragments (Cy3-C0C1 and Cy3-C0C1f) bound to the thin filaments and displayed mod

115 on the thin filament similar to that of the Cy3-C0C3 fragment, the shorter fragments were unable to

116 Dynamic imaging of thin filament-bound Cy3-C0C3 molecules demonstrated that these fragments dif

117 Nucleic acids labeled only with Cy3 can often be used to monitor interactions with unlab

118 wo fluorophores used in aCGH-usually Cy5 and Cy3-can be observed as a bias within the intensity distr

119 majority of GFP-N-WASP undergoing FRET with Cy3-Cdc42 is localized within a transferrin receptor- an

120 vidually transfected TSHR(GFP) and TSHR(Myc):Cy3 cells cultured together and also by acceptor photobl

121 localized, contaminating fluorescence in the Cy3 channel on several commercial and in-house printed m

122 fluorescence microscopy to directly observe Cy3-Cof1 and Cy3-Cof2 interacting with actin filaments i

123 microscopy to directly observe Cy3-Cof1 and Cy3-Cof2 interacting with actin filaments in real time d

124 nt maximum of 28-fold for Cy5 and 4-fold for Cy3, compared to avidin-coated glass substrates.

125 ular trafficking of AAV-2 using fluorescent (Cy3)-conjugated viral particles and molecular techniques

126 en fluorescent protein (GFP)-tagged EGFR and Cy3-conjugated anti-phosphotyrosine antibodies.

127 inals of hippocampal neurons prelabeled with CY3-conjugated antibodies directed against lumenal epito

128 (FRET) using fluorescein isothiocyanate- and Cy3-conjugated monoclonal antibodies (mAbs) that were di

129 FITC-conjugated CD31 and LYVE-1 followed by Cy3-conjugated secondary antibody to quantify corneal bl

130 from Xenopus embryos 1 d after injection of Cy3-conjugated tubulin into one of the blastomeres of tw

131 g carcinoma cell line A549 was infected with Cy3-conjugated viruses for 10 min followed by a 1-h incu

132 Immunization with Cy3 conjugates induces a rapid Cy3-specific gammadelta T

133 to an E. coli 30S initiation complex (30SIC(Cy3)) containing Cy3-labeled initiation factor 2 complex

134 Hemalaun, TUNEL as well as stainings with Cy3-coupled anti-sphingosine or anti-ceramide antibodies

135 Attachment of Cy3, Cy3B and Cy5 to the 5-position of thymidine by an e

136 We have now applied single-molecule FRET to Cy3, Cy5 double-labeled LacI-DNA loops diffusing freely

137 the visible fluorophores (e.g., fluorescein, Cy3, Cy5), it should find broad applicability in FRET as

138 lative fluorescence of DNAs labeled with the Cy3, Cy5, Alexa Fluor 555, and Alexa Fluor 647 dyes and

139 Fluorescent molecular rotor dyes, including Cy3, Cy5, and Alexa Fluor 555, dissolved in micron-sized

140 served increased initial lifetimes of single Cy3, Cy5, and Alexa488 fluorophores.

141 ogue dramatically enhance photostability for Cy3, Cy5, and their conformationally restrained congener

142 Cyanines (Cy3, Cy5, Cy3B) are the most utilized dyes for single-mo

143 ye labels (ROX, rhodamine 6G, HEX, FAM, TET, Cy3, Cy5, TAMRA) attached to oligonucleotide strands is

144 to anti-TRPC protein antibodies labeled with cy3-cy5 pairs.

145 of the dye pair used, with the cyanine pair Cy3-Cy5 showing the least amount of fluctuations.

146 rs (TMR-ATTO647N, Cy3-ATTO647N, TMR-Cy5, and Cy3-Cy5), we find that this phenomenon depends on the na

147 iment whereby immobilized L7Ae protein binds Cy3-Cy5-labeled RNA from free solution.

148 Here we show that these rigidly inserted Cy3/Cy5 chromophores also exhibit two additional useful

149 primer-template DNA constructs labeled with Cy3/Cy5 donor-acceptor Forster resonance energy transfer

150 Fluorophore Cy3/Cy5 fluorescence intensities were analyzed both thro

151 hese measurements probe the distance between Cy3/Cy5 fluorophores that label the ends of a short (15-

152 Using Cy3/Cy5 FRET efficiency to monitor binding of Mg(2+), we

153 ling the three stems of the DNAzyme with the Cy3/Cy5 FRET pair two stems at a time in order to gain d

154 However, no change was observed in the Cy3/Cy5 signal intensity ratio between continuously mixe

155 the colour channel opposite of their design (Cy3/Cy5).

156 or-acceptor pairs, fluorescein/rhodamine and Cy3/Cy5, as a function of peptide concentration and dono

157 ingle-molecule FRET (smFRET) measurements on Cy3/Cy5-labeled primer-template (p/t) DNA constructs in

158 9 V), the conductance of the shorter chains, Cy3(+)-Cy7(+), increases with length (negative attenuati

159 (Cy7) to pentamethine (Cy5) and trimethine (Cy3) cyanines via homogeneous, acid-base-catalyzed nucle

160 Proteins are covalently labeled with Cy2 and Cy3 DIGE and detected simultaneously with a rotary confo

161 s indicate that fluorescence is modulated by Cy3-DNA interactions in a sequence-dependent manner.

162 Hybridization resulted in SPCE due to Cy3-DNA into the prism.

163 rescence resonance energy transfer between a Cy3 donor and Cy5 acceptor fluorescent pair placed on op

164 (FRET) between a pair of fluorescent probes (Cy3 donor and Cy5 acceptor) placed on opposite sides of

165 y acceptor photobleaching, works for the GFP-Cy3 donor-acceptor pair and allows the full quantitation

166 By labeling three arms of the junction with Cy3 (donor), Cy5 (acceptor 1), and Cy5.5 (acceptor 2), d

167 fficient intramolecular energy transfer from Cy3 donors to stoichiometrically amplify the fluorescenc

168 ong-lived Cy5 dark state in conjunction with Cy3 donors, we demonstrate image extraction from a large

169 med dyedrons) comprised of multiple cyanine (Cy3) donors coupled to a single malachite green (MG) acc

170 and genomic DNAs with fluorescently labeled Cy3-dUTP and potentially be useful for diagnostic applic

171 e colors of the crystals by attaching either Cy3 dye (pink) or Cy5 dye (blue-green) to the components

172 ion fragments originating from probe DNA and Cy3 dye are enriched in microspot centers, correlating w

173 mer-forming pyrene pair as a donor and sulfo-Cy3 dye as an acceptor, which demonstrated remarkable 75

174 that use the environmental sensitivity of a Cy3 dye attached to a DNA substrate.

175 riments all samples are labelled with either Cy3 dye or Cy5 dye.

176 Either AlexaFluor 488 or Cy3 dye was attached to maltose binding protein (MBP) an

177 printed DNA distributions by tracking bound Cy3 dye, DNA base, and phosphate specific ion fragments

178 NA binding protein that the magnitude of the Cy3 enhancement is dependent on both the protein as well

179 perties of dye-conjugated FDNs (Cy5-FDNs and Cy3-FDNs) were characterized using single-molecule fluor

180 and then compared to analogous data from the Cy3/fluorescein FRET pair.

181 Both Cy3 fluorescence and in situ hybridization of siRNA corr

182 The time-courses obtained by monitoring Cy3 fluorescence display a distinct lag phase that incre

183 Cy3-labeled reference DNA which undergoes a Cy3 fluorescence enhancement upon protein binding.

184 Using Cy3 fluorescence in vivo imaging, we showed that unlike

185 Using a novel method to calibrate the Cy3 fluorescence intensity as a function of hRPA positio

186 abeled proteins because of an enhancement of Cy3 fluorescence intensity that results when the protein

187 Although Cy3 fluorescence is enhanced upon contacting most protei

188 Here, using a site-specific Cy3 fluorescence probe in the human beta2-adrenergic rec

189 ated remarkable 75-fold enhancement of sulfo-Cy3 fluorescence upon target capturing.

190 ilaments were labeled with an amino-specific Cy3 fluorescent dye and were visualized on a quartz slid

191 ber) by conjugating Ad capsids directly with Cy3 fluorescent dye, permitting the trafficking of the c

192 ed a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to

193 nd whose substrate strand is modified with a Cy3 fluorophore at the 5' end and a molecular quencher a

194 fluctuations in fluorescence intensity of a Cy3 fluorophore attached to the end of ssDNA.

195 t fluorescence lifetime analysis of a single Cy3 fluorophore attached to the promoter region of the D

196 of a reference DNA labeled at its end with a Cy3 fluorophore.

197 therefore been used between fluorescein and Cy3 fluorophores attached to the ends of helical arms to

198 nt dNTPs that contain up to approximately 45 Cy3 fluorophores per 1 kb DNA.

199 The affinity of Cy3 for a T.A basepair (with the dye attached to T) was

200 The gQD/Cy3 FRET pair served as an internal standard, while the

201 These Cy3 FRET time-courses are identical with those obtained

202 D-7HP resin for the improved purification of Cy3-gal from the crude extract.

203 adsorption/desorption to efficiently purify Cy3-gal using macroporous resins.

204 behavior (40-80 degrees C) of the recovered Cy3-gal were performed over time, and the first-order ki

205 Cyanidin-3-O-galactoside (Cy3-gal) is the most widespread anthocyanin that has bee

206 pproximately 10(8) M(-1)s(-1)) observed with Cy3-Hfq was followed by a slow transition (0.5 s(-1)) to

207 Its high specificity makes (Ni(2+)-NTA)(2)-Cy3 ideal for detecting His(6) proteins in complex mixtu

208 The cyanine dye Cy3 is a popular fluorophore used to probe the binding o

209 d with fluorescein and Cy3 suggests that the Cy3 is close to the helical axis of the DNA.

210 An increase in fluorescence intensity of Cy3 is observed at the single-molecule level, reflecting

211 e absence of uranyl, the fluorescence of the Cy3 is quenched by both AuNP and the molecular quencher.

212 ientation of the protein with respect to the Cy3 label on the DNA.

213 Electrokinetic injection of fluorescent dye (Cy3) labeled oligonucleotide target into a microfluidic

214 Analysis of Cy3-labeled Abeta42 monomer adsorption on a quartz subst

215 Upon infection of enucleated cells with Cy3-labeled Ad, the majority of Ad capsid trafficked to

216 Moreover, single-molecule imaging of a Cy3-labeled agonist, [Lys(7), Cys(8)]dermorphin, illustr

217 entified using antisulfotyrosine primary and Cy3-labeled antimouse secondary antibodies.

218 luorescently labeled by exchange of a single Cy3-labeled calmodulin into the neck region of one head.

219 Cy3-labeled cyclodextrins were immobilized on a glass su

220 We studied the emission due to Cy3-labeled DNA oligomers bound to complementary unlabel

221 ditions to incorporate up to ~50 fluorescent Cy3-labeled dNTPs per kilobase into a ssDNA chain.

222 FL-labeled ferritin at the membrane, whereas Cy3-labeled ferritin was found both at the membrane and

223 showed effective tumor-targeting by a novel Cy3-labeled folic acid (FA)-derivatized DNA dendrimer na

224 ere we show that SP-D enhances the uptake of Cy3-labeled fragments of DNA and DNA-coated beads by U93

225 n cultured endothelial cells (ECs), TA145, a cy3-labeled homologue of RP748, localized to alpha(v)bet

226 When added exogenously, (125)I- or Cy3-labeled IGFBP-5 is capable of cellular entry and nuc

227 S initiation complex (30SIC(Cy3)) containing Cy3-labeled initiation factor 2 complexed with GTP leads

228 were characterized using smFRET between the Cy3-labeled L1 stalk of the large ribosomal subunit and

229 of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escher

230 Hybridization of Cy3-labeled oligonucleotide targets provided the proximi

231 rnalization of anionic liposome-encapsulated Cy3-labeled oligonucleotides (AL-Cy3ONs) by hippocampal

232 Anionic liposome-mediated internalization of Cy3-labeled oligonucleotides by neurons and several othe

233 ergy transfer (FRET) between (Ni(2+)-NTA)(2)-Cy3-labeled proteins and suitable donors/acceptors provi

234 h1 antibody, intracellular trafficking using Cy3-labeled rAAV2, and blocking assays for proteasome an

235 DNA tails was examined by competition with a Cy3-labeled reference DNA which undergoes a Cy3 fluoresc

236 examined by competition titrations with the Cy3-labeled reference DNA.

237 synthesis, using ribosomes containing either Cy3-labeled ribosomal protein L11 and A- or P-site Cy5-l

238 almonella was performed and visualized using Cy3-labeled secondary probes in a sandwich-type assay fo

239 (Ec) SSB is bound to surface-immobilized 3'-Cy3-labeled ssDNA, a fluctuating FRET signal is observed

240 er, and hybridization with the complementary Cy3-labeled target DNA was achieved.

241 served as donors that were LRET-paired with Cy3-labeled target DNA.

242 tions is first demonstrated by hybridizing a Cy3-labeled target.

243 tissue pretreatment and hybridization with a Cy3-labeled telomeric repeat complementing (CCCTAA)3 pep

244 Cy3-labeled tilmanocept exhibited high specificity bindi

245 sensor assembly consists of QDs coupled with Cy3-labelled MBP bound to beta-cyclodextrin-Cy3.5.

246 lls bind a fluorescent derivative of leptin (Cy3-leptin).

247 ffects on spot drying, with high-density DNA-Cy3 located in spot centers and nonhomogeneous DNA distr

248 Complete separation of the Cy3-modified reporter strand is thus detected as an incr

249 the loading strand, the enzyme displaces the Cy3-modified reporter, which will bind to a capture or c

250 luorescence photobleaching recovery, using a Cy3-modified, noncompetitive mAb specific for IL-1RI (M5

251 ion of tissue sections in mice receiving the Cy3-MORF/tat/trastuzumab nanoparticle showed evidence of

252 -terminus-KS-AT-MT1-KR-ACP) one NRPS module (Cy3-MT2-PCP3-TE-C-terminus)], was used as a model system

253 different conformation, from that of either Cy3 or Cy5 attached by 3-atom tethers, with the long axe

254 Cy3 or Cy5 dye-labeled DNA exhibited reduced fluorescenc

255 al properties of DNA oligomers, labeled with Cy3 or Cy5, when bound to quartz surfaces coated with me

256 3'-CCA-containing RNAs with the fluorophores Cy3 or Cy5.

257 r fluorophore Cy5, while donor fluorophores (Cy3 or EPI) were attached to HPMA copolymer side chains

258 the fluorescence intensity of fluorophores (Cy3 or fluorescein) attached to the 5'-end of a series o

259 tly bound to the PFC molecules, specifically Cy3-PFC and CypHer5-PFC conjugates.

260 by a relative intensity analysis of the red (Cy3 photoluminescence (PL)) and green (gQD PL) color cha

261 Hence, use of the Cy3 PIFE effect for quantitative studies may require car

262 e stalled intermediates of motors containing Cy3-pRNA and Cy5-DNA.

263 ding to DNA molecules sparsely labelled with Cy3 results in an increase in fluorescence intensity due

264 led with 3,3'-Diethyloxacarbocyanine iodide (CY3), Rhodamine 6G (R6G), and 3,3'-Diethylthiadicarbocya

265 ing splint ligation to doubly label the RPR (Cy3-RPRC domain and Cy5-RPRS domain), we used native mas

266 elf-complementary DNA duplex 5'-labeled with Cy3 shows that the fluorophore is stacked onto the end o

267 teraction with the target as the fluorescent Cy3 signal is quenched by the gold nanoparticle and only

268 ithms, allowed the spectral contributions of Cy3 signal, glass, and contaminating fluorescence to be

269 ckground array signal, normalize the Cy5 and Cy3 signals, score levels of differential hybridization,

270 g glomerular filtration, endocytic uptake of Cy3-siRNA by PTCs was rapid and extensive, and significa

271 nization with Cy3 conjugates induces a rapid Cy3-specific gammadelta T cell IL-17 response.

272 The simulations also showed that stacking of Cy3 straightens the DNA while stabilizing the first base

273 ed material for electron microscopy and with Cy3-streptavidin to label the neurons with fluorescence

274 exes terminally labeled with fluorescein and Cy3 suggests that the Cy3 is close to the helical axis o

275 sfer (FRET) between GFP-tagged KIR2DL1 and a Cy3-tagged generic anti-phosphotyrosine monoclonal antib

276 This is very similar to the behavior of Cy3 terminally attached to DNA and suggests that the eff

277 n increase in the fluorescence anisotropy of Cy3 terminally attached to one of the helical arms of a

278 nert) to quench the triplet excited state of Cy3, the most ubiquitous green emissive dye utilized in

279 IP-1 links two fluorophores (fluorescein and Cy3) through an Fe(II)-cleavable endoperoxide bridge, wh

280 Furthermore, Cy3-tilmanocept binding was markedly reduced on macropha

281 nfocal microscopy revealed colocalization of Cy3-tilmanocept with the macrophage membrane MR and bind

282 of other proteins, allowing (Ni(2+)-NTA)(2)-Cy3 to be used as a probe in crude cell extracts and as

283 scence resonance energy transfer (FRET) from Cy3 to Cy5 since the two ends of (dT)(66) are in close p

284 rgy transfer (smFRET) between (Cy5)EF-G and (Cy3)tRNALys, we studied the translational elongation dyn

285 ribution was determined by microinjection of Cy3 tubulin into GFP-transfected cells.

286 Cells were injected with Cy3-tubulin to fluorescently label MTs and were enucleat

287 modified with capture probe 2 and Raman dye (Cy3) via hybridization with the corresponding target seq

288 ency increased when the solution of AMCA-DNA-Cy3 was placed between two quartz plates coated with sil

289 were adsorbed onto a cellulose support, and Cy3 was used as a green channel acceptor for Survival Mo

290 Donkey anti-human IgG labeled with Cy3 was used for bevacizumab immunoreactivity detection.

291 Annexin V-Cy3 was used to detect phosphatidylserine translocation

292 idine tail and labeled with an acceptor dye (Cy3) was immobilized on the nanocrystals via a noncovale

293 denovirus, labeled with the fluorescent dye (Cy3), was rapidly and widely distributed on epithelial s

294 For the hydrophilic dye Cy3, we have demonstrated that the fluorescence intensit

295 One reporter is modified with cyanine 3 (Cy3), whereas the other is modified with a spectrally pa

296 the common single molecule fluorescence dye Cy3, which is highly susceptible to this destructive pat

297 y required for FRET-sensitized emission from Cy3, which served as an analytical signal.

298 ised tissues via conjugation of streptavidin-Cy3, which targeted the probe biotin moiety, and immunoh

299 y required for LRET-sensitized emission from Cy3, which was used as the detection signal.

300 nomenon but requires a direct interaction of Cy3 with the protein, and the magnitude of the effect is